CN105962375A - Hericium erinaceus active lactobacillus preparation and preparation method thereof - Google Patents
Hericium erinaceus active lactobacillus preparation and preparation method thereof Download PDFInfo
- Publication number
- CN105962375A CN105962375A CN201610312047.0A CN201610312047A CN105962375A CN 105962375 A CN105962375 A CN 105962375A CN 201610312047 A CN201610312047 A CN 201610312047A CN 105962375 A CN105962375 A CN 105962375A
- Authority
- CN
- China
- Prior art keywords
- preparation
- hericium erinaceus
- polysaccharide
- powder
- pers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 240000000588 Hericium erinaceus Species 0.000 title claims abstract description 70
- 235000007328 Hericium erinaceus Nutrition 0.000 title claims abstract description 70
- 238000002360 preparation method Methods 0.000 title claims abstract description 60
- 241000186660 Lactobacillus Species 0.000 title claims abstract description 15
- 229940039696 lactobacillus Drugs 0.000 title claims abstract description 15
- 150000004676 glycans Chemical class 0.000 claims abstract description 51
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 51
- 239000005017 polysaccharide Substances 0.000 claims abstract description 51
- 241000894006 Bacteria Species 0.000 claims abstract description 47
- 239000000843 powder Substances 0.000 claims abstract description 35
- 235000013406 prebiotics Nutrition 0.000 claims abstract description 17
- 238000000855 fermentation Methods 0.000 claims abstract description 13
- 230000004151 fermentation Effects 0.000 claims abstract description 13
- 230000000694 effects Effects 0.000 claims abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 62
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 48
- 244000309464 bull Species 0.000 claims description 29
- 239000004310 lactic acid Substances 0.000 claims description 24
- 235000014655 lactic acid Nutrition 0.000 claims description 24
- 229960004756 ethanol Drugs 0.000 claims description 18
- 239000001963 growth medium Substances 0.000 claims description 18
- 238000004108 freeze drying Methods 0.000 claims description 17
- 230000004913 activation Effects 0.000 claims description 16
- 238000000108 ultra-filtration Methods 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 239000000047 product Substances 0.000 claims description 12
- 239000012153 distilled water Substances 0.000 claims description 11
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 10
- 238000007710 freezing Methods 0.000 claims description 9
- 230000008014 freezing Effects 0.000 claims description 9
- 235000013336 milk Nutrition 0.000 claims description 9
- 239000008267 milk Substances 0.000 claims description 9
- 210000004080 milk Anatomy 0.000 claims description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Natural products OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 8
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 8
- 238000004090 dissolution Methods 0.000 claims description 8
- 229920001542 oligosaccharide Polymers 0.000 claims description 8
- 239000002244 precipitate Substances 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 8
- 239000000725 suspension Substances 0.000 claims description 8
- 241000577951 Hydnum Species 0.000 claims description 7
- 239000002671 adjuvant Substances 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 150000002482 oligosaccharides Chemical class 0.000 claims description 7
- 238000011081 inoculation Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- -1 Citric acid Hydrogen Chemical class 0.000 claims description 5
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 5
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 5
- 238000011177 media preparation Methods 0.000 claims description 5
- 241000186016 Bifidobacterium bifidum Species 0.000 claims description 4
- 229920002261 Corn starch Polymers 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 4
- 210000000481 breast Anatomy 0.000 claims description 4
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
- 239000008120 corn starch Substances 0.000 claims description 4
- 239000006071 cream Substances 0.000 claims description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 229910052739 hydrogen Inorganic materials 0.000 claims description 4
- 239000001257 hydrogen Substances 0.000 claims description 4
- 239000002054 inoculum Substances 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 4
- 229910052748 manganese Inorganic materials 0.000 claims description 4
- 239000011572 manganese Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 4
- 229920000053 polysorbate 80 Polymers 0.000 claims description 4
- 239000013049 sediment Substances 0.000 claims description 4
- 150000003384 small molecules Chemical class 0.000 claims description 4
- 239000001632 sodium acetate Substances 0.000 claims description 4
- 235000017281 sodium acetate Nutrition 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 241000283690 Bos taurus Species 0.000 claims description 3
- 241001635690 Erinaceus amurensis Species 0.000 claims description 3
- 244000199866 Lactobacillus casei Species 0.000 claims description 3
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 3
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 3
- 229940017800 lactobacillus casei Drugs 0.000 claims description 3
- 239000011593 sulfur Substances 0.000 claims description 3
- 229910052717 sulfur Inorganic materials 0.000 claims description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 2
- 244000017020 Ipomoea batatas Species 0.000 claims description 2
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 2
- 241000194017 Streptococcus Species 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 230000001186 cumulative effect Effects 0.000 claims description 2
- 229920001592 potato starch Polymers 0.000 claims description 2
- 210000000582 semen Anatomy 0.000 claims description 2
- 235000020183 skimmed milk Nutrition 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 241001504539 Cercopithecus diana Species 0.000 claims 1
- 230000000968 intestinal effect Effects 0.000 abstract description 12
- 230000036541 health Effects 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 239000006041 probiotic Substances 0.000 abstract description 4
- 235000018291 probiotics Nutrition 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 150000001875 compounds Chemical class 0.000 abstract description 3
- 230000006870 function Effects 0.000 abstract description 3
- 210000002784 stomach Anatomy 0.000 abstract description 3
- 244000052616 bacterial pathogen Species 0.000 abstract description 2
- 241000123222 Hericium Species 0.000 abstract 3
- 210000002249 digestive system Anatomy 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 210000004877 mucosa Anatomy 0.000 abstract 1
- 208000024891 symptom Diseases 0.000 abstract 1
- 239000000203 mixture Substances 0.000 description 8
- 239000002131 composite material Substances 0.000 description 6
- 241000194020 Streptococcus thermophilus Species 0.000 description 5
- 244000068988 Glycine max Species 0.000 description 4
- 235000010469 Glycine max Nutrition 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000282693 Cercopithecidae Species 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 2
- 241000289667 Erinaceus Species 0.000 description 2
- 244000060234 Gmelina philippensis Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 208000010643 digestive system disease Diseases 0.000 description 2
- 235000012489 doughnuts Nutrition 0.000 description 2
- 201000006549 dyspepsia Diseases 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 208000017897 Carcinoma of esophagus Diseases 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 208000014540 Functional gastrointestinal disease Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 208000007882 Gastritis Diseases 0.000 description 1
- 208000018522 Gastrointestinal disease Diseases 0.000 description 1
- 241000186605 Lactobacillus paracasei Species 0.000 description 1
- 206010030155 Oesophageal carcinoma Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 208000003100 Pseudomembranous Enterocolitis Diseases 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 208000007107 Stomach Ulcer Diseases 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 201000007637 bowel dysfunction Diseases 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 208000019902 chronic diarrheal disease Diseases 0.000 description 1
- 208000023652 chronic gastritis Diseases 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 208000000718 duodenal ulcer Diseases 0.000 description 1
- 201000005619 esophageal carcinoma Diseases 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000001408 fungistatic effect Effects 0.000 description 1
- 206010017758 gastric cancer Diseases 0.000 description 1
- 201000005917 gastric ulcer Diseases 0.000 description 1
- 230000007661 gastrointestinal function Effects 0.000 description 1
- 210000005095 gastrointestinal system Anatomy 0.000 description 1
- 208000018685 gastrointestinal system disease Diseases 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 230000000774 hypoallergenic effect Effects 0.000 description 1
- 208000033065 inborn errors of immunity Diseases 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- JCQLYHFGKNRPGE-FCVZTGTOSA-N lactulose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 JCQLYHFGKNRPGE-FCVZTGTOSA-N 0.000 description 1
- 229960000511 lactulose Drugs 0.000 description 1
- PFCRQPBOOFTZGQ-UHFFFAOYSA-N lactulose keto form Natural products OCC(=O)C(O)C(C(O)CO)OC1OC(CO)C(O)C(O)C1O PFCRQPBOOFTZGQ-UHFFFAOYSA-N 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 208000028529 primary immunodeficiency disease Diseases 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a hericium erinaceus active lactobacillus preparation and a preparation method of the hericium erinaceus active lactobacillus preparation. The preparation is prepared from the following components in parts by weight: 25 to 30 parts of hericium polysaccharide, 25 to 30 parts of active lactobacillus powder, 1 to 5 parts of prebiotics and 50 to 60 parts of auxiliary materials. The invention further provides the preparation method of the preparation. The preparation method comprises the following steps of (1) preparing the hericium polysaccharide; (2) preparing the active lactobacillus powder; (3) preparing a finished product. The preparation disclosed by the invention has the advantages that the active preparation simultaneously integrates the healthcare function of the hericium polysaccharide on a human digestive system, beneficial active bacteria generated through combined fermentation of various lactobacillus and the prebiotics benefiting growth and breeding of intestinal flora, so that a compound preparation with the functions of nourishing and invigorating stomach is provided, and the preparation has the effects of protecting mucosa of digestive tract, enhancing probiotics, inhibiting pathogenic bacteria, treating both symptoms and root causes, treating diseases and preserving health; the preparation is conveniently carried and eaten, and is suitable for extensive people.
Description
Technical field
The present invention relates to a kind of health product, be specifically related to a kind of with Hericium Erinaceus Polysaccharide, biodiasmin powder and prebiotics,
And it is aided with a kind of preparation with multiple health care that adjuvant is made and preparation method thereof.
Background technology
Polysaccharide is the living polymer compound that a class has 3-D solid structure, is the important composition of cell receptor,
Identify and transmission information, it is achieved the physiological process of living things catalysis and material conversion plays very important effect.Hericium erinaceus (Bull. Ex Fr.) Pers.
(Hericium erinaceu), it is exactly famous dietotherapeutic fungus since ancient times, delicious food can not only be cooked, be again auxiliary
Help to treat and cure dyspepsia, gastric ulcer, the health food of the digestive system disease such as duodenal ulcer, esophageal carcinoma, gastric cancer,
And there is good tonic effect.The most at home and abroad, Hericium erinaceus (Bull. Ex Fr.) Pers. is the most extensively applied to curing the disease of gi system
With tumor.Research shows, Hericium Erinaceus Polysaccharide is effect position that it is main, is the elite place of Hericium erinaceus (Bull. Ex Fr.) Pers., has: 1) suppress swollen
Tumor grows；2) facilitating digestion road ulcer and the reparation of various chronic gastritis；3) treatment hepatitis；4) Leukocytes after Chemotherapy number is improved
Amount, regulates body's immunity.Result in clinical application is it has been proved that Hericium Erinaceus Polysaccharide is without any toxicity, side effect.
Lactobacillus is the major microorganisms of human body intestinal canal normal flora, has a lot of beneficial effect to human body, including
Exclusion, suppress external pathogenic bacterium, the nutrition such as vitamin is provided, produce amylase, protease etc. and contribute to the enzyme of digestion, decompose
Poisonous or carcinogen (nitrous ammonium), produces organic acid, reduces intestinal pH and promote the normal creepage of gastrointestinal functions of intestinal, stimulate exempting from of body
Epidemic disease system also improves immunity.Lactobacillus, at the amount reproduction of intestinal, can treat pseudomembranous enteritis (because a large amount of use resists
Give birth to element and cause), treatment chronic diarrhea and constipation, reduce cholesterol, the generation of prophylaxis of cancer.
Prebiotics is original one or more beneficial bacterias (probiotic bacteria) in referring to selectively promote human body intestinal canal
The material of growth and breeding, is increased by the breeding of probiotics, suppresses harmful bacterial growth, thus reaches to adjust intestinal microbial population, promotees
Enter the purpose of body health.Prebiotics not by human consumption and absorption, common are oligomeric xylose, lactulose, sucrose oligosaccharide,
Cotton seed oligosaccharide, dextrinosan, Semen Maydis oligosaccharide, soybean oligo saccharide etc..
Summary of the invention
It is an object of the invention to provide a kind of composite health care type containing Hericium erinaceus (Bull. Ex Fr.) Pers. extract, Lactobacillus and prebiotics
Preparation and preparation method thereof.
Make full use of Hericium Erinaceus Polysaccharide to gastrointestinal system prevention and treatment of diseases effect, extraction Hericium erinaceus (Bull. Ex Fr.) Pers. elite portion
Position, uses multiple probiotic combination fermentation technique altogether, and manual intervention introduces intestinal beneficial flora, has been simultaneously introduced soybean oligo saccharide
With the prebiotics that oligomeric xylose etc. is of value to Body normal flora propagation, the continuous proliferation for intestinal bacteria provides material base
Plinth.
A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation in the present invention, by Hericium Erinaceus Polysaccharide, biodiasmin powder, prebiotics and auxiliary
Material composition, shared weight portion is respectively as follows: Hericium Erinaceus Polysaccharide 25～30 parts, biodiasmin powder 25～30 parts, prebiotics 1～5 parts
With adjuvant 50～60 parts.
Described Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation, described lactic acid bacteria is streptococcus thermophilus, Bulgarian Lactobacillus, dry
Lactobacillus paracasei, bacillus bifidus, lactic acid bacteria combined ferment agent is two kinds or three kinds or multiple combination therein, and its inoculum concentration is breast
The 3 ~ 6% of acid bacteria fermentation cumulative volume.
Described Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation, described prebiotics is the one in soybean oligo saccharide and oligomeric xylose
Or two kinds of combinations.
The preparation method of described Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation, described adjuvant is starch, can be wheaten starch,
Corn starch, sweet potato starch etc., or a kind of or in combination of two or more.
The preparation method of described Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation, is carried out as steps described below: (1) Hericium Erinaceus Polysaccharide
Preparation；(2) preparation of biodiasmin powder；(3) finished product is prepared.
The preparation method of described Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation, prepares Hericium Erinaceus Polysaccharide in accordance with the following steps: A. monkey
The pretreatment of head raw material；B. the dissolution of Hericium Erinaceus Polysaccharide；C. little molecule is removed in ultrafiltration；D. the precipitate with ethanol of Hericium Erinaceus Polysaccharide；E. vacuum
Lyophilization；
A. the pretreatment of raw material: take the Hericium erinaceus (Bull. Ex Fr.) Pers. entity of dry constant weight, pulverize, be placed in 8～16 times of volumes 60～95% ethanol molten
Soaking 18～30 h(in liquid preferred, 20～24 h are soaked in ethanol solution in be placed in 9～12 times of volumes 80～95%, more excellent
Choosing, be placed in 95% ethanol solution of 10 times of volumes and soak 22 h), with remove fatty, part single (few) is sugared and fat-soluble color
Element, air-dries at residue cool place；
B. the dissolution of Hericium Erinaceus Polysaccharide: pretreated residue air-dry after, the selective extraction time is 1～2 h, temperature be 60～
100 DEG C, water (distilled water) material ratio is 20～30 mL/g(v/w), 80～120 mesh filtered through gauze, filtering residue repeats to extract 2～3 times；
C. little molecule is removed in ultrafiltration: merged by the foregoing polysaccharide solution repeatedly extracted, and using ultrafilter membrane molecular weight is 1
The hollow cellulose film of ten thousand carries out ultrafiltration, removes the small-molecule substance of less than 10,000；
D. the precipitate with ethanol of Hericium Erinaceus Polysaccharide: add dehydrated alcohol in the extracting solution after ultrafiltration concentration, until the ethanol terminal of solution is dense
Degree is 60～90%(preferably, adds dehydrated alcohol until solution endpoint concentration is 75～85%, more preferably, adds dehydrated alcohol
Until solution endpoint concentration is 80%), 4 DEG C stand 24 h, centrifugal, take sediment fraction, lyophilization；
E. vacuum lyophilization: 3 h at first polysaccharide sample being positioned over-20 DEG C, freezing 6 more than h at then proceeding to-80 DEG C,
Then proceed to vacuum freeze drier be dried, obtain Hericium Erinaceus Polysaccharide.
The preparation method of described Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation, prepares biodiasmin powder: A. in accordance with the following steps
Culture medium preparation and sterilizing；B. the activation of strain；C. inoculation fermentation；D. lyophilization, makes powder；
A. culture medium preparation and sterilizing:
(1) proliferated culture medium (MRS culture medium) formula is: peptone 10.0g；Carnis Bovis seu Bubali cream 10.0g；Yeast extract 5.0g；Citric acid
Hydrogen diammonium 2.0g；Glucose 20.0g；Tween 80 1.0mL；Sodium acetate 5.0g；Dipotassium hydrogen phosphate 2.0g；Magnesium sulfate 0.58g；Sulfur
Acid manganese 0.25g；Distilled water 1000mL；PH is adjusted to 6.2 ~ 6.6, at 121 DEG C, sterilizing 20 min；
(2) strain activation and culture base: taking the fresh milk that healthy cow produces, centrifugal (5000 rpm, 15 min), filtered through gauze must take off
Fat breast (or skimmed milk milk powder, prepare according to the ratio distilled water of 1: 7), it is sub-packed in triangular flask or loads in fermentation tank,
At 108 DEG C, sterilizing 15 min；
B. the activation of strain: it is complete that the strain of Storage in refrigerator accesses spawn activity after passing on 3～5 times in strain activation and culture base
Recover；
C. inoculation fermentation: the strain recovered completely by vigor, by fermentating liquid volume 3～the inoculum concentration of 6%, is linked into MRS and cultivates
In base, at 37 DEG C～40 DEG C after constant temperature quiescent culture 8～14 h, centrifugal (4 DEG C, 8000～10000 rpm, 10 min), abandon
Supernatant, adds defatted milk powder according to the amount of 65～95 g/L, fully mixes, make bacteria suspension；
D. lyophilization, makes powder: above-mentioned bacteria suspension is first placed in 4 DEG C of balances 1 h, 3 h at being then put in-20 DEG C, so
After proceed to-80 DEG C at freezing 6 h, then proceed to vacuum freeze drier and be dried, prepare biodiasmin powder.
The preparation method of described Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation, prepares finished product: weigh monkey respectively in accordance with the following steps
Head mushroom polysaccharide, biodiasmin powder, prebiotics and adjuvant, make tablet through dry method.
Product of the present invention is provided simultaneously with the nutrition health-care functions of Hericium Erinaceus Polysaccharide, the prebiotic effect of active bacteria formulation and prebiotics
Selective proliferative effect, collection three's unification, the Trinity, it is provided that a kind of nourishing the stomach stomach invigorating, there is the guarantor of continuous proliferation effect
Strong type compound formulation；In addition, the excellent results of the present invention is also manifested by:
(1) not only expand the utilization ways of Hericium erinaceus (Bull. Ex Fr.) Pers., improved the added value of Hericium erinaceus (Bull. Ex Fr.) Pers., too increase the product of lactobacillus preparation
Type, there are no this product in the market；
(2) product of the present invention carries and instant, and suitable population is extensive, especially to stomaches such as dyspepsia, low, the constipation of appetite
Bowel dysfunction person, and hypoimmunity, rhythm of life are nervous, diet does not has rule, is susceptible to suffer from the professional population of gastrointestinal disease,
Better, there is good Social benefit and economic benefit.
Detailed description of the invention
Describe technical scheme in detail below in conjunction with embodiment and experimental example, but protection domain is not limited to this.
The preparation method of 1 one kinds of Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparations of embodiment, step is as follows:
(1) preparation of Hericium Erinaceus Polysaccharide: the pretreatment of A. hedgehog hydnum raw material；B. the dissolution of Hericium Erinaceus Polysaccharide；C. ultrafiltration is removed little
Molecule；D. the precipitate with ethanol of Hericium Erinaceus Polysaccharide；E. vacuum lyophilization；
A. the pretreatment of hedgehog hydnum raw material: take Hydnum erinaceus sporophore 1 kg of dry constant weight, pulverizes, is placed in 95% ethanol of 15 times of volumes
Solution soaks 24 h, destroys cell wall and remove fatty, part single (few) sugar and fat-soluble pigment, centrifugal (3000 rpm, 20
Min), air-dry at residue cool place；
B. the dissolution of Hericium Erinaceus Polysaccharide: the residue after Feng Ganing, the selective extraction time is 2 h, and temperature is 90 DEG C, and water (distilled water) is expected
Ratio is 25 ml/g(v/w), 120 mesh filtered through gauze, filtering residue repeats to extract 2 times；
C. little molecule is removed in ultrafiltration: the polysaccharide solution extracted 2 times merges, and using ultrafilter membrane molecular weight is the doughnut of 10,000
Element film carries out ultrafiltration, removes the small-molecule substance of less than 10,000；
The precipitate with ethanol of D Hericium Erinaceus Polysaccharide: the extracting solution after ultrafiltration is centrifuged, takes and reset and add dehydrated alcohol, until solution endpoint concentration
Being 80%, 4 DEG C stand 24 h, centrifugal, take sediment fraction, lyophilization；
E. vacuum lyophilization: 3 h at first polysaccharide sample being positioned over-20 DEG C, freezing 6 more than h at then proceeding to-80 DEG C,
Then proceed to vacuum freeze drier be dried, obtain Hericium Erinaceus Polysaccharide；
(2) preparation of biodiasmin powder
A. culture medium preparation and sterilizing:
(1) proliferated culture medium (MRS culture medium) formula is: peptone 10.0g；Carnis Bovis seu Bubali cream 10.0g；Yeast extract 5.0g；Citric acid
Hydrogen diammonium 2.0g；Glucose 20.0g；Tween 80 1.0mL；Sodium acetate 5.0g；Dipotassium hydrogen phosphate 2.0g；Magnesium sulfate 0.58g；Sulphuric acid
Manganese 0.25g；Distilled water 1000mL；PH is adjusted to 6.6, at 121 DEG C, sterilizing 20 min；
(2) strain activation and culture base: extracting degreasing breast milk powder, the distilled water of the ratio according to 1: 7 is prepared, and is sub-packed in 1000 mL tri-
In the bottle of angle, at 108 DEG C, sterilizing 15 min；
B. the activation of strain: it is the most extensive that the strain of Storage in refrigerator accesses spawn activity after passing on 5 times in strain activation and culture base
Multiple；
C. inoculation fermentation: being made into by the strain after activation completely and be made into composite bacteria agent capable according to certain mass ratio, its composite bacteria agent capable is
Lactobacillus casei and the mixture of streptococcus thermophilus, lactobacillus casei: streptococcus thermophilus=1:1(w/w), by fermentating liquid volume 5%
Amount composite bacteria agent capable is accessed in MRS culture medium, at 37 DEG C after constant temperature quiescent culture 12 h, centrifugal (4 DEG C, 10000 rpm, 10
Min), abandon supernatant, add defatted milk powder according to the amount of 80 g/L, fully mix, make bacteria suspension；
D. lyophilization, makes powder: above-mentioned bacteria suspension is first placed in 4 DEG C of balances 1 h, 3 h at being then put in-20 DEG C, so
After proceed to-80 DEG C at freezing 6 h, then proceed to vacuum freeze drier and be dried, prepare biodiasmin powder；
(3) finished product is prepared
Weigh Hericium Erinaceus Polysaccharide, biodiasmin powder, oligomeric xylose and corn starch respectively, shared weight portion be respectively as follows: 30 parts,
30 parts, 2 parts and and 55 parts, make tablet through dry method.
The preparation method of 2 one kinds of Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparations of embodiment, step is as follows:
(1) preparation of Hericium Erinaceus Polysaccharide: the pretreatment of A. hedgehog hydnum raw material；B. the dissolution of Hericium Erinaceus Polysaccharide；C. ultrafiltration is removed little
Molecule；D. the precipitate with ethanol of Hericium Erinaceus Polysaccharide；E. vacuum lyophilization；
A. the pretreatment of hedgehog hydnum raw material: take Hydnum erinaceus sporophore 1 kg of dry constant weight, pulverizes, is placed in 95% ethanol of 10 times of volumes
Solution soaks 22 h, destroys cell wall and remove fatty, part single (few) sugar and fat-soluble pigment, centrifugal (3000 rpm, 20
Min), air-dry at residue cool place；
B. the dissolution of Hericium Erinaceus Polysaccharide: the residue after Feng Ganing, the selective extraction time is 1.5 h, and temperature is 90 DEG C, water (distilled water)
Material ratio is 25 mL/g(v/w), 100 mesh filtered through gauze, filtering residue repeats to extract 3 times；
C. little molecule is removed in ultrafiltration: the polysaccharide solution extracted 3 times merges, and using ultrafilter membrane molecular weight is the doughnut of 10,000
Element film carries out ultrafiltration, removes the small-molecule substance of less than 10,000；
The precipitate with ethanol of D Hericium Erinaceus Polysaccharide: the extracting solution after ultrafiltration is centrifuged, takes and reset and add dehydrated alcohol, until solution endpoint concentration
Being 80%, 4 DEG C stand 24 h, centrifugal, take sediment fraction, lyophilization；
E. vacuum lyophilization: 3 h at first polysaccharide sample being positioned over-20 DEG C, freezing 6 more than h at then proceeding to-80 DEG C,
Then proceed to vacuum freeze drier be dried, obtain Hericium Erinaceus Polysaccharide；
(2) preparation of biodiasmin powder
A. culture medium preparation and sterilizing:
(1) proliferated culture medium (MRS culture medium) formula is: peptone 10.0g；Carnis Bovis seu Bubali cream 10.0g；Yeast extract 5.0g；Citric acid
Hydrogen diammonium 2.0g；Glucose 20.0g；Tween 80 1.0mL；Sodium acetate 5.0g；Dipotassium hydrogen phosphate 2.0g；Magnesium sulfate 0.58g；Sulfur
Acid manganese 0.25g；Distilled water 1000mL；PH is adjusted to 6.6, at 121 DEG C, sterilizing 20 min；
(2) strain activation and culture base: taking the fresh milk that healthy cow produces, centrifugal (5000 rpm, 15 min), filtered through gauze must take off
Fat breast, is sub-packed in triangular flask or loads in fermentation tank, at 108 DEG C, and sterilizing 15 min；
B. the activation of strain: it is the most extensive that the strain of Storage in refrigerator accesses spawn activity after passing on 5 times in strain activation and culture base
Multiple；
C. inoculation fermentation: the strain after activation completely is made into and is made into composite bacteria agent capable, by fermentating liquid volume according to certain mass ratio
The amount of 5% is linked in MRS culture medium, and its composite bacteria agent capable is Bulgarian Lactobacillus, streptococcus thermophilus and bacillus bifidus
Mixture, Bulgarian Lactobacillus: streptococcus thermophilus: bacillus bifidus=6: 1: 1(w/w), constant temperature quiescent culture 12h at 37 DEG C
After, centrifugal (4 DEG C, 10000 rpm, 10 min), abandon supernatant, add defatted milk powder according to the amount of 80 g/L, fully mix, system
Become bacteria suspension；
D. lyophilization, makes powder: above-mentioned bacteria suspension is first placed in 4 DEG C of balances 1 h, 3 h at being then put in-20 DEG C, so
After proceed to-80 DEG C at freezing 6 h, then proceed to vacuum freeze drier and be dried, prepare biodiasmin powder；
(3) finished product is prepared
Weighing Hericium Erinaceus Polysaccharide, biodiasmin powder, soybean oligo saccharide and corn starch respectively, shared weight portion is respectively as follows: 28
Part, 28 parts, 2 parts and and 55 parts, make tablet through dry method.
The bacteriostatic test of test example 1 gained of the present invention a kind of Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation:
Choosing the intestinal bacteria such as escherichia coli, Salmonella, staphylococcus aureus and Hemolytic streptococcus is experimental strain, will
Above-mentioned strain is inoculated in plain agar culture medium, cultivates 24 h for 37 DEG C, and picking colonies typical is inoculated in fresh broth bouillon
In, cultivate 18 h for 37 DEG C, standby；Under aseptic technique, extract the 50 each bacterium solution of μ L and coat in plain agar culture medium,
And the title of the good added strain of labelling, the most equidistant punching, bore dia is 6 mm；Respectively by embodiment 1 and embodiment 2 gained
Tablet pulverizes, and adds water and makes the solution that concentration is 1 g/mL, draws two kinds of solution of 100 μ L respectively and adds to hole, is positioned over
24 h, the antibacterial circle diameter (mm) around observation register hole is cultivated in 37 DEG C of calorstats.
Fungistatic effect judges: recording according to document, antibacterial circle diameter >=20 mm is the quickest；15 ~ 19 mm are Gao Min；10~
14 mm are quick in being；10 below mm are hypoallergenic.
Result is as shown in table 1:
The 1 two kinds of solution of the table bacteriostasis to intestinal bacteria:
As shown in Table 1, products obtained therefrom of the present invention reaches 10.5 ~ 16 mm to the antibacterial circle diameter of above-mentioned four kinds of intestinal bacteria, wherein
More preferable to the bacteriostasis of Salmonella and staphylococcus aureus；And understand by contrast, the antibacterial circle diameter of embodiment 2 is the highest
In embodiment 1, illustrate that the bacteriostasis of embodiment 2 is the most prominent.
It should be pointed out that, the detailed description of the invention more representational example that is the present invention, it is clear that the skill of the present invention
Art scheme is not limited to above-described embodiment, it is also possible to have many variations.Those of ordinary skill in the art, the most public with present invention institute
What what that open or according to file written description was undoubted obtained, all it is considered as this patent scope of the claimed.
The present invention completes by Shandong Province's nature fund assistance (No. ZR2015CL008).
Claims (8)
1. a Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation, it is characterised in that by Hericium Erinaceus Polysaccharide, biodiasmin powder, prebiotics and
Adjuvant forms, and shared weight portion is respectively as follows: Hericium Erinaceus Polysaccharide 25～30 parts, biodiasmin powder 25～30 parts, prebiotics 1～5
Part and adjuvant 50～60 parts.
2. according to the Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation described in claim 1, it is characterised in that described lactobacillus powder is thermophilic
Streptococcus, Bulgarian Lactobacillus, lactobacillus casei, bacillus bifidus, lactic acid bacteria combined ferment agent is two kinds or three therein
Planting or multiple combination, its inoculum concentration is the 3 ~ 6% of lactic acid bacteria fermentation cumulative volume.
3. according to the Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation described in claim 1, it is characterised in that described prebiotics is Semen sojae atricolor
One in oligosaccharide and oligomeric xylose or two kinds of combinations.
4. according to the preparation method of the Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation described in claim 1, it is characterised in that described is auxiliary
Material is starch, can be wheaten starch, corn starch, sweet potato starch etc., or a kind of or in combination of two or more.
5. according to the preparation method of the Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation described in claim 1, it is characterised in that according to following
Step is carried out: the preparation of (1) Hericium Erinaceus Polysaccharide；(2) preparation of biodiasmin powder；(3) finished product is prepared.
The preparation method of Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation the most according to claim 5, it is characterised in that according to following step
Suddenly Hericium Erinaceus Polysaccharide is prepared: the pretreatment of A. hedgehog hydnum raw material；B. the dissolution of Hericium Erinaceus Polysaccharide；C. little molecule is removed in ultrafiltration；D. monkey
The precipitate with ethanol of head mushroom polysaccharide；E. vacuum lyophilization；
A. the pretreatment of raw material: take the Hericium erinaceus (Bull. Ex Fr.) Pers. entity of dry constant weight, pulverize, be placed in 8～16 times of volumes 60～95% ethanol molten
Soaking 18～30 h(in liquid preferred, 20～24 h are soaked in ethanol solution in be placed in 9～12 times of volumes 80～95%, more excellent
Choosing, be placed in 95% ethanol solution of 10 times of volumes and soak 22 h), with remove fatty, part single (few) is sugared and fat-soluble color
Element, air-dries at residue cool place；
B. the dissolution of Hericium Erinaceus Polysaccharide: pretreated residue air-dry after, the selective extraction time is 1～2 h, temperature be 60～
100 DEG C, water (distilled water) material ratio is 20～30 mL/g(v/w), 80～120 mesh filtered through gauze, filtering residue repeats to extract 2～3 times；
C. little molecule is removed in ultrafiltration: merged by the foregoing polysaccharide solution repeatedly extracted, and using ultrafilter membrane molecular weight is 1
The hollow cellulose film of ten thousand carries out ultrafiltration, removes the small-molecule substance of less than 10,000；
D. the precipitate with ethanol of Hericium Erinaceus Polysaccharide: add dehydrated alcohol in the extracting solution after ultrafiltration concentration, until the ethanol terminal of solution is dense
Degree is 60～90%(preferably, adds dehydrated alcohol until solution endpoint concentration is 75～85%, more preferably, adds dehydrated alcohol
Until solution endpoint concentration is 80%), 4 DEG C stand 24 h, centrifugal, take sediment fraction, lyophilization；
E. vacuum lyophilization: 3 h at first polysaccharide sample being positioned over-20 DEG C, freezing 6 more than h at then proceeding to-80 DEG C,
Then proceed to vacuum freeze drier be dried, obtain Hericium Erinaceus Polysaccharide.
7. according to the preparation method of the Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation described in claim 5, it is characterised in that according to as follows
Step prepares biodiasmin powder: A. culture medium is prepared and sterilizing；B. the activation of strain；C. inoculation fermentation；D. freezing dry
Dry, make powder；
A. culture medium preparation and sterilizing:
(1) proliferated culture medium (MRS culture medium) formula is: peptone 10.0g；Carnis Bovis seu Bubali cream 10.0g；Yeast extract 5.0g；Citric acid
Hydrogen diammonium 2.0g；Glucose 20.0g；Tween 80 1.0mL；Sodium acetate 5.0g；Dipotassium hydrogen phosphate 2.0g；Magnesium sulfate 0.58g；Sulfur
Acid manganese 0.25g；Distilled water 1000mL；PH is adjusted to 6.2 ~ 6.6, at 121 DEG C, sterilizing 20 min；
(2) strain activation and culture base: taking the fresh milk that healthy cow produces, centrifugal (5000 rpm, 15 min), filtered through gauze must take off
Fat breast (or skimmed milk milk powder, prepare according to the ratio distilled water of 1: 7), it is sub-packed in triangular flask or loads in fermentation tank,
At 108 DEG C, sterilizing 15 min；
B. the activation of strain: it is complete that the strain of Storage in refrigerator accesses spawn activity after passing on 3～5 times in strain activation and culture base
Recover；
C. inoculation fermentation: the strain recovered completely by vigor, by fermentating liquid volume 3～the inoculum concentration of 6%, is linked into MRS and cultivates
In base, at 37 DEG C～40 DEG C after constant temperature quiescent culture 8～14 h, centrifugal (4 DEG C, 8000～10000 rpm, 10 min), abandon
Supernatant, adds defatted milk powder according to the amount of 65～95 g/L, fully mixes, make bacteria suspension；
D. lyophilization, makes powder: above-mentioned bacteria suspension is first placed in 4 DEG C of balances 1 h, 3 h at being then put in-20 DEG C, so
After proceed to-80 DEG C at freezing 6 h, then proceed to vacuum freeze drier and be dried, prepare biodiasmin powder.
8. according to the preparation method of the Hericium erinaceus (Bull. Ex Fr.) Pers. active lactic acid bacteria preparation described in claim 5, it is characterised in that according to as follows
Step prepares finished product: weighs Hericium Erinaceus Polysaccharide, biodiasmin powder, prebiotics and adjuvant respectively, makes tablet through dry method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610312047.0A CN105962375A (en) | 2016-05-12 | 2016-05-12 | Hericium erinaceus active lactobacillus preparation and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610312047.0A CN105962375A (en) | 2016-05-12 | 2016-05-12 | Hericium erinaceus active lactobacillus preparation and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105962375A true CN105962375A (en) | 2016-09-28 |
Family
ID=56992380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610312047.0A Pending CN105962375A (en) | 2016-05-12 | 2016-05-12 | Hericium erinaceus active lactobacillus preparation and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105962375A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109207549A (en) * | 2018-11-05 | 2019-01-15 | 福建拓天生物科技有限公司 | A method of using the high efficiency extraction hericium erinaceum polysaccharide of zymotechnique |
| CN109770146A (en) * | 2019-01-18 | 2019-05-21 | 广东微量元素生物科技有限公司 | A kind of auxiliary repairs the probiotics crystallite medicinal granules of powder and preparation method thereof of gastric mucosa |
| CN110537655A (en) * | 2019-09-20 | 2019-12-06 | 贵州山环菌草科技有限公司 | preparation method of hericium erinaceus fermented beverage |
| CN116998724A (en) * | 2023-06-28 | 2023-11-07 | 安琪生物科技有限公司 | Preparation and application of lactobacillus living bacteria and gastrodia elata polysaccharide composition |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1603402A (en) * | 2003-11-03 | 2005-04-06 | 东北农业大学 | Method for preparing bifidobacterium lyophilized powder and its products and use |
| CN102021162A (en) * | 2009-09-19 | 2011-04-20 | 菲伯纳生物医药有限责任公司 | High-activity bifidobacterium powder and preparation method thereof |
| CN104770639A (en) * | 2015-04-09 | 2015-07-15 | 福建农大食品安全科技有限公司 | Complex lactobacillus bifidus based hericium erinaceus polysaccharide chewable tablet and preparation method thereof |
-
2016
- 2016-05-12 CN CN201610312047.0A patent/CN105962375A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1603402A (en) * | 2003-11-03 | 2005-04-06 | 东北农业大学 | Method for preparing bifidobacterium lyophilized powder and its products and use |
| CN102021162A (en) * | 2009-09-19 | 2011-04-20 | 菲伯纳生物医药有限责任公司 | High-activity bifidobacterium powder and preparation method thereof |
| CN104770639A (en) * | 2015-04-09 | 2015-07-15 | 福建农大食品安全科技有限公司 | Complex lactobacillus bifidus based hericium erinaceus polysaccharide chewable tablet and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 詹益兴等: "《绿色精细化工-天然产品的制造法》", 31 January 2008, 科学技术文献出版社 * |
| 阎世英编著: "《酵素决定健康》", 31 January 2016, 中国医药科技出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109207549A (en) * | 2018-11-05 | 2019-01-15 | 福建拓天生物科技有限公司 | A method of using the high efficiency extraction hericium erinaceum polysaccharide of zymotechnique |
| CN109770146A (en) * | 2019-01-18 | 2019-05-21 | 广东微量元素生物科技有限公司 | A kind of auxiliary repairs the probiotics crystallite medicinal granules of powder and preparation method thereof of gastric mucosa |
| CN110537655A (en) * | 2019-09-20 | 2019-12-06 | 贵州山环菌草科技有限公司 | preparation method of hericium erinaceus fermented beverage |
| CN116998724A (en) * | 2023-06-28 | 2023-11-07 | 安琪生物科技有限公司 | Preparation and application of lactobacillus living bacteria and gastrodia elata polysaccharide composition |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104544086B (en) | A kind of health food improving intestinal microbial population and preparation method thereof | |
| CN104509864B (en) | A kind of have nutritional health food improving gastrointestinal function and preparation method thereof | |
| CN104543679A (en) | Probiotic-containing health food and preparation method thereof | |
| CN104770816A (en) | Watermelon juice probiotic fermented beverage and preparation method thereof | |
| CN105146614A (en) | Functional Chinese dwarf cherry seed ferment, ferment beverage and production method of ferment beverage | |
| CN104970101A (en) | Probiotics troche and preparation method thereof | |
| CN105123931A (en) | Probiotic foodstuff and preparation method thereof | |
| CN105028646A (en) | Freeze-dried tablets and preparation method thereof | |
| CN105123925A (en) | Freeze-dried foodstuff and preparation method thereof | |
| CN104543680A (en) | Health food for resisting fatigue and improving gastrointestinal functions and preparation method of health food | |
| CN105285285A (en) | Blueberry pressed candy and preparation method thereof | |
| CN105076440A (en) | Freeze-dried fruit and vegetable yoghurt tablet and preparation method thereof | |
| CN105028638A (en) | Fruit and vegetable lactic acid bacteria tablet and preparation method thereof | |
| CN105076648A (en) | Blueberry tablet candy with beautifying and antibacterial efficacy and preparation method thereof | |
| CN105962375A (en) | Hericium erinaceus active lactobacillus preparation and preparation method thereof | |
| CN103053738A (en) | Tea-processing herba cistanche and preparation method thereof | |
| CN105028637A (en) | Fruit puree tablet containing probiotics and preparation method for fruit puree tablet | |
| CN105123949A (en) | Yoghourt tablet containing live bacteria and preparation method thereof | |
| CN105104529A (en) | Lactobacillus food and preparation method thereof | |
| CN104982526A (en) | Yoghourt slice and preparation method thereof | |
| CN105962378A (en) | Preparation method of active lactobacillus preparation containing hericium polysaccharides | |
| CN105802876B (en) | A kind of composite probiotics ferment clover tender shoots powder preparation and its preparation method and application | |
| CN105123932A (en) | Fruit and vegetable probiotic tablet and preparation method thereof | |
| CN105076413A (en) | Fruit and vegetable tablet containing lactic acid bacteria and preparation method of fruit and vegetable tablet | |
| CN104886569A (en) | An active probiotic fermented drink and a preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160928 |