CN104544086B

CN104544086B - A kind of health food improving intestinal microbial population and preparation method thereof

Info

Publication number: CN104544086B
Application number: CN201510033640.7A
Authority: CN
Inventors: 李霄汉; 陈可翔
Original assignee: Guangzhou Little Hill Health Technology Co Ltd
Current assignee: Henan Xiaoshan Health Technology Service Co., Ltd
Priority date: 2015-01-23
Filing date: 2015-01-23
Publication date: 2016-10-05
Anticipated expiration: 2035-01-23
Also published as: CN104544086A

Abstract

The invention discloses a kind of health food improving intestinal microbial population and preparation method thereof, from increasing probiotic bacteria kind and quantity, increase probiotic bacteria nutrient substance, suppression harmful bacteria growth and increasing many-sided intestinal microbial populations that adjust such as body immunity, improve gastrointestinal function.With probiotic bacteria powder such as Lactobacillus plantarum as primary raw material, science compounds fungi extracts, modified dietary fiber；It is remarkably improved body immunity, anti-inflammatory and antalgic, there is the Saussurea involucrata culture extract etc. of the functions such as antioxidation, radioprotective and resisting fatigue, and then while increasing beneficial bacteria of intestinal tract flora kind, significantly enhance endogenous and that external source probiotic bacteria is in human body intestinal canal colonization ability and resident time, effectively inhibit growth and the breeding of harmful intestinal tract bacteria especially gram negative bacteria, fully have adjusted the composition of beneficial bacteria of intestinal tract flora, prepare a kind of probiotic strong, significantly improve body immunity, the health food of intestinal microbial population can be improved.

Description

A kind of health food improving intestinal microbial population and preparation method thereof

Technical field

The present invention relates to health food, be specifically related to a kind of health food improving intestinal microbial population and preparation method thereof.

Background technology

Improve gastrointestinal function to include relieving constipation, regulating intestinal canal flora, facilitating digestion, have four kinds of functions of auxiliary protection to gastric mucosa injury. The alternate balance of intestinal bacterium is affected by the interaction etc. of physiology, food, medicine and the intestinal of host, and various factors all can break The balance that bad bacterium is alternate, causes intestinal to be preponderated from probiotics and turns to harmful bacteria to preponderate, thus causes many intestinal tract diseases.Cause This, increase the quantity of intestinal beneficial bacterium, reduces harmful bacteria quantity, is to ensure that the key point that gastrointestinal health operates.

Dysbacteriosis (dysbacteriosis) refers in the normal flora of body a part that the ratio between each strain occurs by a relatively large margin Changing and exceed the state of normal range, consequent disease, referred to as dysbacteriosis or flora replace disease (microbial selection and substitution).During dysbacteriosis, cause superinfection or superinfection (superinfection) more, I.e. in the treatment of primary infection, there occurs the infection of another kind of new pathogenic bacterium.Use antibiotic is more common in dysbacteriosis With Chronic consumptions etc..After widely applying broad ectrum antibiotic for a long time, most of sensitive organisms and normal flora are suppressed Or kill, but fastbacteria then obtains survival advantage and amount reproduction causes a disease, as resistant Staphylococcus aureus causes diarrhoea, deteriorated blood Disease, causes thrush, vaginitis, intestinal and anal infection to the candida albicans bacterium that antibiotic is insensitive.

At present, the mode of intestinal microbial population is adjusted mainly with probiotic supplemented kind and quantity, probiotic supplemented desired nutritional material (benefit The raw factor), suppress or kill harmful intestinal tract bacteria (mainly gram negative bacteria), strengthen body immunity to improve macrophage The modes such as the phagocytosis to harmful bacteria are to reach the purpose of intestinal microbial population balance, and Chinese patent CN 10155908A discloses one Planting the preparation method of the probiotics preparation of blood fat reducing and regulating intestinal canal flora, its step is that (1) is by Kefir lactobacillu plantarurn MA2 carries out High Density Cultivation, obtains fermentation liquid, and its cell density is not less than 10⁸cfu/mL；(2) above-mentioned fermentation liquor is centrifugal receives Collection obtains thalline, adds protective agent, is lyophilized into mycopowder；(3) in the mycopowder of lyophilizing, oligosaccharide mixture, mix homogeneously are added Obtain probiotics preparation finished product.These product also have the physiological function of regulating intestinal canal flora, Ke Yizhi while having hypolipemic function Hyperlipidemic conditions and regulating intestinal canal flora, and nontoxic, safety, long shelf-life treated and prevented, capsule, microcapsule, punching can be made into Agent, tablet etc..Chinese patent CN 102511707B health food disclosing a kind of regulating intestinal canal and preparation method thereof, including The component of following weight portion: natural plant extracts 50-60%；Oligofructose 8-12%；Microbial ecological agent probiotic bacteria 2-3%； Mel 20-30%；Wherein, described natural plant extracts include Fructus Fici extract, Prunus mume Sieb. Et Zucc. extract, tamarind extract, Fructus Jujubae extract, its weight ratio is 10: 1: 1: 1.Natural plant extracts is pulverized；Weigh Mel to add in evaporating dish Heat, to boiling, is treated that honey surface rises bubble, is taken off immediately；Load weighted natural plant extracts is mixed with oligofructose, probiotic bacteria Close, to wait honey temperature to be down to when 30-40 DEG C add the when of mixing, make doughy soft material；By soft for the dough lived Material is placed, and makes spice and honey component be sufficiently mixed moist, makes spheroidal pill.This product with pure natural plant extract, Oligofructose, microbial ecological agent are primary raw material, regulating intestinal canal function on the premise of not stimulating intestinal, reach prophylactic treatment just Secret effect.Chinese patent CN 103054938A discloses a kind of oral formulations regulating Biology Barrier of Gastrointestinal Tract imbalance, and it relates to one Oral formulations.The invention solves the problems that and widely apply broad ectrum antibiotic regulation Biology Barrier of Gastrointestinal Tract imbalance for a long time, produce drug resistance The problem of property.The oral formulations of the present invention is to be extracted by inulin, Radix Ginseng extract, Rhizoma Atractylodis Macrocephalae extract, Poria extract, Radix Glycyrrhizae Thing is made.The present invention is a kind of contributes to improving the oral formulations scientific formula of gastrointestinal function, raw material is easy to get, prepare conveniently, effect Substantially, side effect is little, and is suitable for industrialized production.

Patent disclosed above is adopted as traditional probiotic bacteria and probiotics factor and combinations thereof, do not separate or turn out probiotic more Strong probiotic bacteria, its probiotic bacteria biological activity is the most less desirable, and traditional probiotics factor is delayed to the proliferation function of probiotic bacteria, And in suppression harmful intestinal tract bacteria, strengthen body immunity and then improve the macrophage phagocytosis to harmful bacteria, to improve intestinal Road beneficial flora aspect research less.

Summary of the invention

Solved by the invention technical problem is that overcomes the existing defect improving gastrointestinal function health product, from increase probiotic bacteria kind and Many-sided adjustment intestinal microbial populations such as quantity, increase probiotic bacteria nutrient substance, suppression harmful bacteria growth and increase body immunity, change Kind gastrointestinal function.With probiotic bacteria powder such as Lactobacillus plantarum as primary raw material, science compounding can repair cell tissue, have anti-routed Infections effect, antioxidation, effect of scavenging radical and anti-aging effects, can be obviously improved human body overall immunity and reinforcement Power, improve the fungi extracts of gastrointestinal function；Modified dietary fiber containing high-load soluble cellulose；It is remarkably improved Body immunity, anti-inflammatory and antalgic, the Saussurea involucrata culture extract with functions such as antioxidation, radioprotective and resisting fatigue etc. are raw material, And then while increasing beneficial bacteria of intestinal tract flora kind, significantly enhance endogenous and external source probiotic bacteria field planting in human body intestinal canal Ability and resident time, effectively inhibit growth and the breeding of harmful intestinal tract bacteria especially gram negative bacteria, fully have adjusted intestinal The composition of road probiotic bacteria flora, prepare a kind of probiotic strong, significantly improve body immunity, the health care of intestinal microbial population can be improved Food.

In order to achieve the above object, the present invention is by the following technical solutions:

A kind of health food improving intestinal microbial population, is prepared by the raw material of following parts by weight:

D-mannital 5-50 part, maltodextrin 5-30 part, microcrystalline Cellulose 5-30 part, probiotic bacteria powder 5-30 part, change Property dietary fiber 5-20 part, oligomeric xylose 3-15 part, hydroxyl isomaltulose 3-15 part, concentrated lactoalbumin 3-15 part, food With fungus extract 2-12 part, Saussurea involucrata culture extract 3-10 part, pectin decomposer 2-10 part, cyclohexaamylose 1-10 part, Citric acid 0.1-3 part, magnesium stearate 0.1-3 part, milk flavour 0.1-3 part, beta-carotene 0.01-3 part；

Preferably, the described health food improving intestinal microbial population, the raw material of following parts by weight prepare:

D-mannital 15-35 part, maltodextrin 10-20 part, microcrystalline Cellulose 10-20 part, probiotic bacteria powder 10-20 part, Modified dietary fiber 8-12 part, oligomeric xylose 7-11 part, hydroxyl isomaltulose 7-11 part, concentrated lactoalbumin 7-11 part, Fungi extracts 5-10 part, Saussurea involucrata culture extract 5-8 part, pectin decomposer 5-7 part, cyclohexaamylose 1.5-5 Part, citric acid 1.5-2.5 part, magnesium stearate 1.5-2.5 part, milk flavour 1.5-2.5 part, beta-carotene 1-2 part；

It is highly preferred that the described health food improving intestinal microbial population, prepare the raw material of following parts by weight:

D-mannital 25 parts, maltodextrin 15 parts, microcrystalline Cellulose 15 parts, 15 parts of probiotic bacteria powder, modified meals are fine Tie up 10 parts, oligomeric xylose 9 parts, hydroxyl isomaltulose 9 parts, concentrated lactoalbumin 9 parts, fungi extracts 8 parts, Herba Saussureae Involueratae Culture extract 7 parts, pectin decomposer 6 parts, cyclohexaamylose 3 parts, citric acid 2 parts, magnesium stearate 2 parts, milk 2 parts of essence, beta-carotene 1.5 parts；

Further, one or more during described probiotic bacteria powder includes but not limited to following probiotic bacteria powder: such as: plant Lactobacillus (Lactobacillus plantarum), bifidobacterium bifidum (Bifidobacterium bifidum), Ying Ershuan Qi bacillus (B.infantis), long bifidus bacillus (B.longum), short bifidus bacillus (B.breve), youth double qi bar Bacterium (B.adolescentis), Lactobacillus bulgaricus (Lactobacillus.Bulgaricus), bacillus acidophilus (L.acidophilus), lactobacillus casei (Lactobacillus Casey), streptococcus thermophilus (Streptococcus thermophilus)；Also one or more in the fungus powder of health food are included, such as: saccharomyces cerevisiae (Saccharomyces cerevisiae), Candida utilis (Cadida atilis), Kluyveromyces lactis (Kluyveromyces lactis), saccharomyces carlsbergensis (Saccharomyces carlsbergensis), peacilomyce hepiahi (Paecilomyces hepiali Chen et Dai, sp.nov), Hirsutella hepiali Chen et Shen (Hirsutella hepiali Chen Et Shen), Ganoderma (Ganoderma lucidum), Ganoderma (Ganoderma sinensis), Ganoderma tsugae (Ganoderma Tsugae), monascus (Monacus anka), Mauve aspergillar (Monacus purpureus)；

Described probiotic bacteria powder probiotics viable bacteria content is: 7x10¹²-9x10¹²cfu/g；

Preferably, described probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 40-50 part, Bifidobacterium longum 30-45 part, bifidobacterium bifidum 25-35 part, bifidobacterium adolescentis 15-20 part, Lactobacillus bulgaricus 15-20 Part, bacillus acidophilus's 10-15 part, lactobacillus casei 8-10 part, streptococcus thermophilus 8-10 part, saccharomyces cerevisiae 8-10 part, produce Protein candidiasis 4-6 part；

Preferably, described Lactobacillus plantarum powder is by Lactobacillus plantarum (Lactobacillus plantarum) tlj-2014 Preparation, this bacterial strain is preserved in the (letter of China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 2nd, 2014 Claiming CGMCC, address is: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3. Institute of Microorganism, Academia Sinica. and postcode: 100101), Deposit number is CGMCC NO.9405, and Classification And Nomenclature is: Lactobacillus plantarum Lactobacillus plantarum.

Further, described modified dietary fiber is that dietary fiber is processed through physics, chemical or biological method and obtained, Soluble fiber cellulose content is high, biological activity strong, have important, the cellulose of positive role to beneficial bacteria of intestinal tract group, and commonly Dietary fiber is compared, and its biological action is more powerful: 1) soluble fiber cellulose content is high, it is easier to utilized by probiotic bacteria, can Improve probiotic bacteria in the growth of intestinal and fertility, increase kind and the quantity of probiotic bacteria flora, reduce large intestine pH value, improve Intestine microenvironment；2) powerful absorbability, after modified, the specific surface area of cellulose increases, and network is enriched, Absorption affinity strengthens, and the organic molecule ability chelating, adsorbing cholesterol and bile acids is higher, the suppression human body absorption to them；3) Ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is higher；4) retentiveness, dilatancy, increasing Consistence is higher and acid and alkali, alkali, the impact of salt, 5) regulate and maintain the resident time of intestinal microbial population, strengthen intestinal digestion and Absorbability, improves body immunity；6) promote gastrointestinal peristalsis, slow down and eliminate the untoward reaction such as flatulence, abdominal distention；7) bag The effect of burying is strong, prevents external environment (oxygen, temperature, illumination, the humidity etc.) factor impact on product quality, stabilizes product The biological activity of product, extends the shelf-life of product；

Preferably, described modified fibre is through life by one or more in inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber Thing enzyme enzymolysis and obtain；

It is highly preferred that the preparation method of described modified dietary fiber comprises the following steps: by inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber 8-10:4-6:3-5:2-4 in mass ratio uniformly mixes, and adds the water of its quality 3-7 times, room temperature 200-300W, 35-40KHz condition supersound extraction 10-15min, then in electric field intensity 20-40kV/cm, burst length 400-500 μ s, pulse frequency High-pressure pulse electric extraction is carried out under the conditions of rate 200-300Hz；It is 4.5-6.5 with breast acid for adjusting pH value, adds mixture quality The enzyme of 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Enzymolysis solution concentrating under reduced pressure, lyophilization, low-temperature grinding are to grain Footpath is that 0.1-0.3mm i.e. obtains modified dietary fiber；

Described enzyme is xylanase, cellulase, laccase, pectase, tannase 5-9:2-4:2-4:2-4:1-3 in mass ratio Uniformly mixing.

Further, described fungi extracts is with repair cell tissue, has antiulcer action, antioxidation, removing Free Radical and anti-aging effects, can be obviously improved human body entirety epidemic disease and exempt from power and physical strength reinforcing, improve the edible fungi of gastrointestinal function Prepare through ultrasonic cleaning, high voltage pulse electric field processing and biological enzymolysis；

Preferably, the preparation method of described fungi extracts comprises the steps: to put Hericium erinaceus (Bull. Ex Fr.) Pers., Semen Ginkgo and Auricularia respectively Enter in the ultrasonic washing unit equipped with 0.3-0.5% sodium bicarbonate solution and clean 5-10min in 200W, 40KHz, drain, cutting Becoming phase equal-specification, length and width is 5-10mm, and the sheet of thickness 3-5mm or fourth, the most in mass ratio 8-10:5-7:4-6 are equal Even mixing, then in-21-25 DEG C of freezing 10-30min, pulverizes immediately, and ground product particle diameter is 0.2-1mm；Add powder The water of quality 3-5 times that minces obtains mixture, and adjusting mixture pH is 4.5-6, in electric field intensity 35-45kV/cm under room temperature, and arteries and veins Rush time 500-700 μ s, carry out high voltage pulse electric field processing under the conditions of pulse frequency 200-400Hz, be warming up to 45～55 DEG C, add Enter the mixed enzyme enzymolysis 4 of mixture weight 0.5-1.2%～6 hours, obtain edible fungi enzymolysis solution, be evaporated to solid content and contain Amount is more than 30%, and lyophilization, low-temperature grinding to particle diameter are that 0.1-0.3mm i.e. obtains fungi extracts；

In described edible fungi enzymolysis solution, edible fungi polysaccharide content is up to 6.82%；

Described mixed enzyme parts by weight consist of: papain 20-30 part, bromelain 20-30 part, glucanase 10-15 Part, xylanase 10-15 part, pentosanase 10-15 part, ficin 5-10 part, midrange thermal stable amylase 5-10 part, pectin Enzyme 5-10 part, tannase 3-5 part.

Further, described Saussurea involucrata culture is purchased from Dalian Puruikang Biotechnology Co, for aubergine lumps granule, The source of kind system " Herba Saussureae Involueratae "；

Further, described Saussurea involucrata culture extract be chilled with Saussurea involucrata culture for raw material, pulverize, microwave-assisted ultrasonic The extract at low temperature technology such as extraction, low temperature enzymolysis, high-pressure pulse electric extraction and prepare, can retain to greatest extent acacetin, Flavonoid substances and other antioxidation, volatile, the thermal sensitivity effective ingredient such as dinatin, rutin, Quercetin；

Preferably, the preparation method of described Saussurea involucrata culture extract comprises the steps: to take Saussurea involucrata culture, immerses and fills quality Percent concentration is in the container of aseptic oligofructose aqueous solution of 0.9-1.1%, takes out, then in-18-23 DEG C of freezings 5-10min；It is crushed to particle diameter 0.3-1mm immediately；Put in container and add the water of 3-5 times of weight or the ethanol of 35-75%, obtaining mixed Compound material, is 3.5-5.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 25-35kV/cm, and burst length 300-500 μ s, High voltage pulse electric field processing is carried out under the conditions of pulse frequency 200-300Hz；Then heating to 30-40 DEG C, insulation, at power Carry out microwave extraction under the conditions of 150-300W, frequency 2000Hz, wherein, each microwave exposure total time 60-80s, be spaced Formula irradiation: irradiation 10s, is spaced 10s, and such irradiation 10 times, simultaneously in power 200-300W, frequency 30-40KHz condition Under carry out ultrasonic assistant extraction；It is continuously heating to 40-50 DEG C, insulation, it is subsequently adding answering of mixed material gross weight 2.5-4% Join enzyme enzymolysis 30-50min, filter, obtain filtrate；Filtering residue rinses 3 times with 1-3 times of weight 76-80 DEG C water, rinsing liquid and filtrate Merge, uniformly mix, at room temperature in electric field intensity 35-45kV/cm, burst length 400-600 μ s, pulse frequency 200-300Hz Under the conditions of carry out high voltage pulse electric field processing and i.e. obtain Saussurea involucrata culture extract；

Described compound enzyme is by pectase, cellulase, tannase, amylase and protease 3-7:3-5:2-4:1-3:1-2 in mass ratio Uniformly mixing.

Described pectin decomposer has agreeable to the taste tart flavour natural, soft and significant antagonistic property, especially makees escherichia coli suppression With the strongest, the most also there is stronger non-oxidizability, can act on when concentration reaches 0.01%, to superoxide radical, hydroxyl freely Base and DPPH free radical all show the strongest elimination effect, and when concentration reaches 1.0%, eradicating efficacy reaches 100%；

The science of described pectin decomposer compounds, and can be effectively improved the palatability of health food of the present invention, and most importantly it is notable Antagonistic property and non-oxidizability avoid oxidation and the microorganism encroach of effective ingredient, stabilized and increased the biological activity of product, Extend the shelf-life of product；

Further, described pectin decomposer is with peel or Fructus Crataegi, Radix Betae, Fructus Mali pumilaes such as Fructus Citri Limoniae, orange, Fructus Citri grandis, mandarin orange, Fructus Vitis viniferaes Pectin is obtained through ultrasonic cleaning, high-voltage pulse extraction, hot water extraction, precipitate with ethanol in waste residue, then prepared through pectinase enzymatic hydrolysis； Preferably, the preparation method of described pectin decomposer, comprise the steps: by fresh Fructus Crataegi marc, Pericarpium Citri grandis, pomace, Megasse is respectively put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and cleans 5-10min, drip in 200W, 40KHz Dry, it is cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, in mass ratio 8-12:3-5:2-4:2-4 Uniformly mixing, then in-21-25 DEG C of freezing 10-30min；Pulverizing immediately, ground product particle diameter is 0.1-1mm；Add The deionized water of ground product quality 3-5 times, mix homogeneously, is 2-3 with Fructus Citri Limoniae acid for adjusting pH value, in electric field intensity under room temperature 35-45kV/cm, burst length 500-700 μ s, carry out high voltage pulse electric field processing, then under the conditions of pulse frequency 200-400Hz Normal pressure boils 30-50min, Bag filter, and filtering residue boils 40-60min with the filtrate normal pressure of its quality 1-3 times, Bag filter, Merging filtrate, kieselguhr filters, makes filtrate clarify, and being evaporated to solid content at 75 DEG C is 6-10%, cools down rapidly To room temperature, add 95% ethanol that pH value is 2-3 of 1.5 times of volumes of concentrated solution, stand 20-30min, make pectin be precipitated out, 4000r/min is centrifuged 5-10min, reclaims precipitation, with the dehydrated alcohol eluting of 2 times of volumes, centrifugal, reclaims precipitation, so grasps Making 2 times, obtain is deposited in 40-60 DEG C of vacuum drying 2-3h, it is thus achieved that pectin, adds 40-50 DEG C of water of pectin quality 4-5 times, Stirring, uniformly mixes, insulation, is 4.5-5.5 with breast acid for adjusting pH value, adds the pectinase enzymatic hydrolysis 2-4h of 0.3-0.8U/mL, Enzymolysis solution filters, and filtrate is enzyme denaturing 10-15min in boiling water, is cooled to room temperature, and ultrafiltration concentration, lyophilization i.e. obtain pectin Thing.

The dosage form of health food of the present invention is oral formulations, can be tablet, powder, capsule or granule, preferably tablet.

Described tablets, transporting, store and easy to carry, character is stable, and dosage is accurate, low cost.

The present invention can improve the health caring food tablet of intestinal microbial population, capsule or granule and can be prepared via a method which, including Following steps:

1) according to formula proportion, D-mannital, maltodextrin, microcrystalline Cellulose, oligomeric xylose, different Fructus Hordei Germinatus ketone are taken respectively Sugar alcohol, concentrated lactoalbumin, fungi extracts, Saussurea involucrata culture extract, milk flavour are pulverized, and cross 60-120 mesh sieve, Then mix 3-15min, obtain mixed powder；

2) by pectin decomposer, citric acid, the beta-carotene aqueous solution making 13-18% soluble in water, as binding agent；

3) in described mixed powder, add described binding agent and make soft material, pelletize with 10-20 mesh sieve, obtain wet granular, put microwave and do In power 3000W, frequency 2450MHz, the dry 3-8min of temperature 55-65 DEG C in dry machine, with the quick granulate of 10-20 mesh sieve；

4) by modified dietary fiber, cyclohexaamylose and probiotic powder mixing 5-10min, probiotic bacteria powder must be embedded；

5) whole good granule mixes 5-10min with embedding probiotic bacteria powder, magnesium stearate, after mix homogeneously, makes tablet, glue Wafer or granule.

Preferably, the preparation method of health caring food tablet of the present invention, comprise the steps:

5) whole good granule mixes 5-10min with embedding probiotic bacteria powder, magnesium stearate, and after mix homogeneously, tabletting, packaging are i.e. Obtain health caring food tablet.

The present invention can improve the preparation method of the health food powder of intestinal microbial population, comprises the steps:

1) press formula proportion, by modified dietary fiber, cyclohexaamylose and probiotic powder mixing 5-10min, obtain embedding probiotic bacteria Powder；

2) according to formula proportion, by D-mannital, maltodextrin, microcrystalline Cellulose, oligomeric xylose, hydroxyl isomaltulose, Concentrated lactoalbumin, fungi extracts, Saussurea involucrata culture extract, pectin decomposer, citric acid are pulverized, and cross 60-120 mesh Sieve, then mixes 3-15min i.e. obtain health food with embedding probiotic bacteria powder, beta-carotene, milk flavour, magnesium stearate Powder.

Lactobacillus plantarum of the present invention (Lactobacillus plantarum) tlj-2014 is preserved in China on July 2nd, 2014 (being called for short CGMCC, address is: city of BeiJing, China Chaoyang District North Star west at Microbiological Culture Collection administration committee common micro-organisms center Road 1 institute 3. Institute of Microorganism, Academia Sinica. postcode: 100101), deposit number is CGMCC NO.9405, classification life Entitled: Lactobacillus plantarum Lactobacillus plantarum.

Described lactobacillus plantarum strain feature is as follows: examine under a microscope, and this bacterial strain is rod-short, and Gram’s staining is positive, Atrichia, does not produce spore；On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circular, limit Edge is more neat.

Physicochemical characteristics is: catalase (-), gelatin liquefaction (-), indole experiment (+), mobility (-), fermentation Aerogenesis (-), nitrate reductase (-), fermentation gas (-), product hydrogen sulfide gas (-), pH4.0MRS culture medium Middle growth (+).

Described Lactobacillus plantarum uses following flow process to carry out selection-breeding:

The original strain that sets out → test tube activation → dithyl sulfate (DES) mutation → nitrosoguanidine (NTG) mutation → etc. Gas ions mutation → flat board primary dcreening operation → shaking flask sieves → mitotic stability test again.

Described starting strain is in MRS dextrose culture-medium, and the throughput rate of its lactic acid is 1.5g/L/d, when medium pH is 3.5 Almost stopping growing, the decomposition rate to sodium nitrite is 0.34mg/h/kg Chinese cabbage.Starting strain is that Li Zheng is collected in Yanchi County, ningxia The greenfeed of Fattening Sheep field, county, acquisition time JIUYUE in 2013 15 days.

In order to improve its production of lactic acid speed, acid-fast ability and the decomposition rate of nitrite, use DES and NTG technology pair successively This strain carries out mutation, and after mutation, bacterial strain uses MRS calcium carbonate flat board to carry out primary dcreening operation, then uses 500mL shake flask fermentation, biological Biosensor analysis instrument carries out multiple sieve to Producing Strain, the lactobacillus plantarum strain that selection-breeding is excellent, then does passage assays, evaluates it and loses Pass stability.

Lactobacillus plantarum tlj-2014 hereditary stability result shows: through continuous passage ten times, property indices all compares surely Fixed, heritability is preferable, and character is not replied, the purpose bacterial strain therefore Lactobacillus plantarum tlj-2014 obtained as selection-breeding.

Empirical tests finds: the production of lactic acid speed of this mutagenic strain can reach 35g/L/d, and this bacterial strain is breast after fermentation in 71 hours Acid concentration reaches 95g/L；Can survive under conditions of pH is 1.80.Degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg (speed of natural fermentation process nitrite accumulation is about 1.1mg/h/kg), it is possible to resistance to 1% cholate.

Therefore using this strain to produce Pickles, whole sweat nitrite concentration is at below 5mg/kg, far below country's mark The content (20mg/kg) of regulation in quasi-GB2714-2003.

Beneficial effect:

The present invention can improve the health food of intestinal microbial population from increasing probiotic bacteria kind and quantity, increase probiotic bacteria nutrient substance, pressing down It is shaped with harmful bacteria growing and increases many-sided intestinal microbial populations that adjust such as body immunity, improving gastrointestinal function.With benefits such as Lactobacillus plantarum Raw bacteria powder is primary raw material, science compounding can repair cell tissue, there is antiulcer action, antioxidation, removing freedom Base effect and anti-aging effects, the edible fungi can be obviously improved human body overall immunity and physical strength reinforcing, improving gastrointestinal function extracts Thing；Modified dietary fiber containing high-load soluble cellulose；Be remarkably improved body immunity, anti-inflammatory and antalgic, have anti- The Saussurea involucrata culture extracts of the functions such as oxidation, radioprotective and resisting fatigue etc. are raw material, and then are increasing beneficial bacteria of intestinal tract flora kind While class, significantly enhance endogenous and that external source probiotic bacteria is in human body intestinal canal colonization ability and resident time, effectively inhibit The growth of harmful intestinal tract bacteria especially gram negative bacteria and breeding, fully have adjusted the composition of beneficial bacteria of intestinal tract flora, prepare A kind of probiotic strong, significantly improve body immunity, the health food of intestinal microbial population can be improved.Concrete know-why is:

1. can to improve the health food probiotic bacteria kind of intestinal microbial population many for the present invention, Lactobacillus plantarum CGMCC NO.9405 therein Production of lactic acid speed can reach 35g/L/d, and this bacterial strain lactic acid concn after fermentation in 71 hours reaches 95g/L；Can be at pH Survive under conditions of 1.80.Degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg (natural fermentation process nitrous acid The speed of salt accumulation is about 1.1mg/h/kg), it is possible to resistance to 1% cholate, its prebiotic performance is more superior.

2. supersound extraction, high-pressure pulse electric are extracted and biological enzymolysis combination of sciences, institute by the modified dietary fiber that prepared by the present invention Modified dietary fiber soluble fiber cellulose content is high, it is easier to utilized by probiotic bacteria, improve probiotic bacteria in the growth of intestinal and numerous Grow ability, increase kind and the quantity of probiotic bacteria flora, reduce large intestine pH value, improve intestine microenvironment；High adsorption capacity, After modified, the specific surface area of cellulose increases, and network is enriched, and absorption affinity strengthens, and chelates, adsorbs cholesterol and bile The organic molecule ability of acids is higher, the suppression human body absorption to them；Ion-exchange capacity strengthens, to metallic element, especially It is that heavy metal element adsorption effect is higher, effectively prevent body weight for humans metal poisoning；Retentiveness, dilatancy, thickening property higher and Acid and alkali, alkali, the impact of salt.Regulation and the resident time of maintenance intestinal microbial population, strengthen digestion and the absorbability of intestinal, carry High body immunity；Effectively facilitate gastrointestinal peristalsis, slow down and eliminate the untoward reaction such as flatulence, abdominal distention；Powerful embedding effect can Prevent external environment (oxygen, temperature, illumination, the humidity etc.) factor impact on product quality, stabilize the biological of product and live Property, extend the shelf-life of product.

3. the fungi extracts that prepared by the present invention is with repair cell tissue, has antiulcer action, antioxidation, removing Free Radical and anti-aging effects, can be obviously improved human body entirety epidemic disease and exempt from power and physical strength reinforcing, improve the edible fungi of gastrointestinal function Obtaining through ultrasonic cleaning, high voltage pulse electric field processing and biological enzymolysis, effective component extraction rate is high, eats in edible fungi enzymolysis solution Being up to 6.82% with granulose content, biological activity is higher, and foodsafety is high, remains without pesticide and worm's ovum.

4. the Saussurea involucrata culture extract that prepared by the present invention is to have analgesia, antiinflammatory, rheumatism；Suppression platelet aggregation, fall Blood fat, improve blood circulation；Immune system is had regulation effect；Also have antioxidation, radioprotective and resisting fatigue etc. in many ways simultaneously The Herba Saussureae Involueratae of the pharmacodynamic action in face be the Saussurea involucrata culture in kind of source be that raw material is chilled, pulverizing, microwave-assisted supersound extraction, The extract at low temperature technology such as low temperature enzymolysis, high-pressure pulse electric extraction and prepare, acacetin, coarse wool globefish can be retained to greatest extent Flavonoid substances and other antioxidation, volatile, the thermal sensitivity effective ingredient such as grass element, rutin, Quercetin, effective ingredient obtains Rate is high, and technique is simple, the time is short, improves raw material availability, reduces production cost, improves production efficiency.Test card Bright, Saussurea involucrata culture extract flavone yield prepared by the present invention is up to 97%, and solid yield is up to 65%.And the training of Herba Saussureae Involueratae cell Foster thing no longer contains the colchicine having side effect to human body, and Ministry of Public Health ratifies Saussurea involucrata culture on May 20th, 2010 For new resource food, to the modernization of Chinese medicine of promotion China, protect wild plant resource, Development of Social Economy significant.

5. the pectin decomposer that prepared by the present invention has agreeable to the taste tart flavour natural, soft and significant antagonistic property, especially to large intestine Bacillus inhibitory action is the strongest, the most also has stronger non-oxidizability, can act on when concentration reaches 0.01%, to super oxygen freely Base, hydroxy radical and DPPH free radical all show the strongest elimination effect, and when concentration reaches 1.0%, eradicating efficacy reaches 100%；The science of pectin decomposer compounds, and can be effectively improved the palatability of health food of the present invention, and most importantly it is significant Antagonistic property and non-oxidizability avoid oxidation and the microorganism encroach of effective ingredient, have stabilized and increased the biological activity of product, Extend the shelf-life of product.

The compounding hydroxyl isomaltulose of science the most of the present invention have low in calories, sugariness natural pure, height endurability, non-cariogenic tooth, More rational negative heat of solution, high stability and non-hygroscopic, improving product quality, extend the shelf life, promote gastro-intestinal digestion With absorption aspect there is excellent characteristic, simultaneously or a kind of excellent bacillus bifidus multiplicaiton factor, although hydroxyl isomaltulose is not Can be utilized by the enzyme system of human body and most microorganism, but utilization can be decomposed by the bacillus bifidus in human body intestinal canal, Promote the growth and breeding of bacillus bifidus, maintain the microecological balance of intestinal, improve the strong of human body overall immunity, beneficially human body Health.

7. the effect of the health food that the present invention can improve intestinal microbial population is the mutual synergistic result of each component, the most former Material function superposition, the science of each raw material components compound, the effect of generation considerably beyond each single component function and the superposition of effect, There is the most advanced and practicality.

8. the preparation method technique of the health food that the present invention can improve intestinal microbial population is simple, easy to operate, low for equipment requirements, can Industrialization and large-scale production, finally mix modified dietary fiber, has played its powerful embedding effect so that the property of product Shape is more stable, the shelf-life is longer.

Detailed description of the invention

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is this Method well known to skilled person.It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, The spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, real without departing substantially from the present invention On the premise of matter and scope, the various changes or the change that carry out the material component in these embodiments and consumption fall within this Bright protection domain.

Prepared by embodiment 1 raw material

1. the preparation of Lactobacillus plantarum powder:

(1) first order seed is cultivated: accessed by Lactobacillus plantarum CGMCC NO.9405 strain in 500 milliliters of shaking flasks, seed culture Base loading amount 100 milliliters, cultivation temperature 37 DEG C, incubation time 24 hours；

(2) secondary seed is cultivated: accesses in 500 milliliters of secondary seed shaking flasks by first order seed according to the inoculum concentration of 10%, cultivates Condition is identical with first order seed；

(3) three grades of seed culture: accessing in 5000 milliliters of three grades of seed flask by secondary seed with 10% inoculum concentration, seed is trained Support base loading amount 1000 milliliters, cultivation temperature 37 DEG C, incubation time 24 hours；

(4) first class seed pot is cultivated: with 5% inoculum concentration, three grades of seeds are accessed the total measurement (volume) first class seed pot as 150L, fermentation Culture medium loading amount 100L, cultivation temperature 37 DEG C, tank pressure 0.05MPa, incubation time 18 hours；

(5) fermentor cultivation: with 5% inoculum concentration, first class seed pot strain is accessed total measurement (volume) is 3 tons of secondary seed tanks, fermentation Culture medium loading amount 2 tons, condition of culture cultivation temperature 37 DEG C, tank pressure 0.05MPa, incubation time 22 hours.Ferment complete fermentation Liquid staticly settles, centrifugal be precipitated thing, then adds the carrier of precipitate quality 0.8 times, mix homogeneously, 50 DEG C of fluid beds Being drying to obtain Lactobacillus plantarum powder, vehicle weight consists of: Yoghourt powder 25 parts, 12 parts of dextrin.

Described seed culture medium quality group becomes: casein 1%, Carnis Bovis seu Bubali cream 1%, yeast extract 0.5%, glucose 0.5%, sodium acetate 0.5%, citric acid diamidogen 0.2%, Tween 800.1%, K₂HPO₄0.2%, MgSO₄.7H₂O 0.02%, MnSO₄.H₂O 0.005%, CaCO₃ 2%, surplus is water, pH6.8.

Described fermentation medium quality group becomes: soybean protein 1%, beef extract 1%, yeast extract 0.5%, glucose 0.5%, Sodium acetate 0.5%, citric acid diamidogen 0.2%, K₂HPO₄0.2%, MgSO₄.7H₂O 0.02%, MnSO₄.H₂O 0.005%, CaCO₃2%, Surplus is water, pH6.8.

In described Lactobacillus plantarum powder, viable bacteria content is 9x10¹²cfu/g。

2. the preparation of modified dietary fiber

The preparation method of described modified dietary fiber comprises the following steps: by inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber 9:5:4:3 in mass ratio uniformly mixes, and adds the water of its quality 5 times, room temperature 300W, 40KHz condition supersound extraction 12min, Then in electric field intensity 30kV/cm, burst length 500 μ s, carry out high-pressure pulse electric extraction under the conditions of pulse frequency 300Hz； It is 5.0 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.2%, in 50 DEG C of enzymolysis 35min；Enzymolysis solution decompression is dense Contracting, lyophilization, low-temperature grinding to particle diameter are that 0.2mm i.e. obtains modified dietary fiber；

Described enzyme is that xylanase, cellulase, laccase, pectase, tannase 7:3:3:3:2 in mass ratio uniformly mixes Close.

3. the preparation of fungi extracts

The preparation method of described fungi extracts comprise the steps: to be respectively put into Hericium erinaceus (Bull. Ex Fr.) Pers., Semen Ginkgo and Auricularia equipped with The ultrasonic washing unit of 0.4% sodium bicarbonate solution cleans 8min in 200W, 40KHz, drains, be cut into phase equal-specification, grow, A width of 5-10mm, the sheet of thickness 3-5mm or fourth, the most in mass ratio 9:6:5 uniformly mix, then in-23 DEG C of freezings 20min, pulverizes immediately, and ground product particle diameter is 0.5mm；The water adding ground product quality 4 times obtains mixture, adjusts mixing Thing pH is 5.2, in electric field intensity 40kV/cm under room temperature, and burst length 600 μ s, carry out high pressure under the conditions of pulse frequency 300Hz Impulse electric field processes, and is warming up to 50 DEG C, the mixed enzyme enzymolysis of addition mixture weight 0.8% 5 hours, obtains edible fungi enzymolysis solution, Being evaporated to solid content is more than 30%, and lyophilization, low-temperature grinding to particle diameter are that 0.2mm i.e. obtains fungi extracts；

Described mixed enzyme parts by weight consist of: papain 25 parts, bromelain 25 parts, glucanase 12 parts, and wood is poly- Carbohydrase 12 parts, pentosanase 12 parts, ficin 8 parts, midrange thermal stable amylase 8 parts, pectase 7 parts, tannase 4 parts.

4. the preparation of Saussurea involucrata culture extract

The preparation method of described Saussurea involucrata culture extract comprises the steps: to take Saussurea involucrata culture, immerses and fills mass percent Concentration is in the container of aseptic oligofructose aqueous solution of 1.0%, takes out, then in-20 DEG C of freezing 8min；It is crushed to grain immediately Footpath 0.5mm；Put in container and add 4 times of weight 40% ethanol, obtain mixed material, with breast acid for adjusting pH value be 4.5, At room temperature in electric field intensity 30kV/cm, burst length 400 μ s, carry out at high-pressure pulse electric under the conditions of pulse frequency 250Hz Reason；Then heat to 35 DEG C, insulation, under the conditions of power 200W, frequency 2000Hz, carry out microwave extraction, wherein, every time Microwave exposure total time 70s, carry out compartment irradiation: irradiation 10s, be spaced 10s, such irradiation 10 times, simultaneously at power 250W, Ultrasonic assistant extraction is carried out under the conditions of frequency 35KHz；It is continuously heating to 45 DEG C, insulation, it is subsequently adding mixed material gross weight The compound enzyme enzymolysis 40min of 3.3%, filters, obtains filtrate；Filtering residue 78 DEG C of water of 2 times of weight rinse 3 times, rinsing liquid and filtrate Merge, uniformly mix, at room temperature in electric field intensity 40kV/cm, burst length 500 μ s, enters under the conditions of pulse frequency 250Hz Horizontal high voltage impulse electric field processes and i.e. obtains Saussurea involucrata culture extract；

Described compound enzyme is uniformly mixed by pectase, cellulase, tannase, amylase and protease 5:4:3:2:1.5 in mass ratio Close.

5. the preparation of pectin decomposer

The preparation method of described pectin decomposer, comprises the steps: fresh Fructus Crataegi marc, Pericarpium Citri grandis, pomace, Radix Betae Slag is respectively put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and cleans 8min in 200W, 40KHz, drains, cutting Becoming phase equal-specification, length and width is 5-10mm, and the sheet of thickness 3-5mm or fourth, in mass ratio 10:4:3:3 uniformly mix, then In-23 DEG C of freezing 20min；Pulverizing immediately, ground product particle diameter is 0.5mm；Add the deionized water of ground product quality 4 times, Mix homogeneously, is 2.5 with Fructus Citri Limoniae acid for adjusting pH value, in electric field intensity 40kV/cm under room temperature, and burst length 600 μ s, pulse frequency Carrying out high voltage pulse electric field processing under the conditions of rate 300Hz, then normal pressure boils 40min, Bag filter, and filtering residue is by its quality 2 Filtrate normal pressure again boils 50min, Bag filter, merging filtrate, and kieselguhr filters, makes filtrate clarify, reduce pressure dense at 75 DEG C Being reduced to solid content is 8%, is rapidly cooled to room temperature, adds 95% ethanol that pH value is 2.5 of 1.5 times of volumes of concentrated solution, Standing 25min, make pectin be precipitated out, 4000r/min is centrifuged 8min, reclaims precipitation, with the dehydrated alcohol eluting of 2 times of volumes, Centrifugal, reclaim precipitation, so operation 2 times, obtain is deposited in 50 DEG C of vacuum drying 2.5h, it is thus achieved that pectin, adds pectic substance Measure 45 DEG C of water of 4.5 times, stirring, uniformly mix, insulation, be 5 with breast acid for adjusting pH value, add the pectase of 0.5U/mL Enzymolysis 3h, enzymolysis solution filters, and filtrate is enzyme denaturing 12min in boiling water, is cooled to room temperature, and ultrafiltration concentration, lyophilization i.e. obtain pectin Analyte.

Lactobacillus plantarum powder that following example are used, modified dietary fiber, fungi extracts, Saussurea involucrata culture extract Thing, pectin decomposer are embodiment 1 to be prepared, and other raw material is commercial commodity.

Embodiment 2

A kind of health caring food tablet improving intestinal microbial population, is prepared by the raw material of following parts by weight:

Described probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 45 parts, bifidobacterium longum 38 parts, bifidobacterium bifidum 30 parts, bifidobacterium adolescentis 18 parts, Lactobacillus bulgaricus 18 parts, bacillus acidophilus 12 parts, Lactobacillus casei 9 parts, streptococcus thermophilus 9 parts, saccharomyces cerevisiae 9 parts, Candida utilis 5 parts；

Described probiotic bacteria powder probiotics viable bacteria content is: 9x10¹²cfu/g；

Preparation method, comprises the steps:

1) according to formula proportion, D-mannital, maltodextrin, microcrystalline Cellulose, oligomeric xylose, different Fructus Hordei Germinatus ketone are taken respectively Sugar alcohol, concentrated lactoalbumin, fungi extracts, Saussurea involucrata culture extract, milk flavour are pulverized, and cross 80 mesh sieves, then Mixing 10min, obtains mixed powder；

2) by soluble in water to pectin decomposer, citric acid, beta-carotene make 15% aqueous solution, as binding agent；

3) in described mixed powder, add described binding agent and make soft material, pelletize with 16 mesh sieves, obtain wet granular, put microwave dryer In be dried 5min in power 3000W, frequency 2450MHz, temperature 60 C, with the 16 quick granulate of mesh sieve；

4) by modified dietary fiber, cyclohexaamylose and probiotic powder mixing 8min, probiotic bacteria powder must be embedded；

5) whole good granule mixes 8min with embedding probiotic bacteria powder, magnesium stearate, after mix homogeneously, tabletting, packs and i.e. obtains guarantor Health food tablet.

Embodiment 3

D-mannital 15 parts, maltodextrin 10 parts, microcrystalline Cellulose 10 parts, 10 parts of probiotic bacteria powder, modified meals are fine Tie up 8 parts, oligomeric xylose 7 parts, hydroxyl isomaltulose 7 parts, concentrated lactoalbumin 7 parts, fungi extracts 5 parts, Herba Saussureae Involueratae Culture extract 5 parts, pectin decomposer 5 parts, cyclohexaamylose 1.5 parts, citric acid 1.5 parts, magnesium stearate 1.5 parts, Milk flavour 1.5 parts, beta-carotene 1 part；

Described probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 40 parts, bifidobacterium longum 30 parts, bifidobacterium bifidum 25 parts, bifidobacterium adolescentis 15 parts, Lactobacillus bulgaricus 15 parts, bacillus acidophilus 10 parts, Lactobacillus casei 8 parts, streptococcus thermophilus 8 parts, saccharomyces cerevisiae 8 parts, Candida utilis 4 parts；

Described probiotic bacteria powder probiotics viable bacteria content is: 7x10¹²cfu/g；

Preparation method, comprises the steps:

1) according to formula proportion, D-mannital, maltodextrin, microcrystalline Cellulose, oligomeric xylose, different Fructus Hordei Germinatus ketone are taken respectively Sugar alcohol, concentrated lactoalbumin, fungi extracts, Saussurea involucrata culture extract, milk flavour are pulverized, and cross 60 mesh sieves, then Mixing 3min, obtains mixed powder；

2) by soluble in water to pectin decomposer, citric acid, beta-carotene make 13% aqueous solution, as binding agent；

3) in described mixed powder, add described binding agent and make soft material, pelletize with 10 mesh sieves, obtain wet granular, put microwave dryer In in power 3000W, frequency 2450MHz, 55 DEG C of dry 3min of temperature, with the 10 quick granulate of mesh sieve；

4) by modified dietary fiber, cyclohexaamylose and probiotic powder mixing 5min, probiotic bacteria powder must be embedded；

5) whole good granule mixes 5min with embedding probiotic bacteria powder, magnesium stearate, after mix homogeneously, tabletting, packs and i.e. obtains guarantor Health food tablet.

Embodiment 4

A kind of health-care edible capsule agent improving intestinal microbial population, is prepared by the raw material of following parts by weight:

D-mannital 35 parts, maltodextrin 20 parts, microcrystalline Cellulose 20 parts, 20 parts of probiotic bacteria powder, modified meals are fine Tie up 12 parts, oligomeric xylose 11 parts, hydroxyl isomaltulose 11 parts, concentrated lactoalbumin 11 parts, fungi extracts 10 parts, Saussurea involucrata culture extract 8 parts, pectin decomposer 7 parts, cyclohexaamylose 5 parts, citric acid 2.5 parts, magnesium stearate 2.5 Part, milk flavour 2.5 parts, beta-carotene 2 parts；

Described probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 50 parts, bifidobacterium longum 45 parts, bifidobacterium bifidum 35 parts, bifidobacterium adolescentis 20 parts, Lactobacillus bulgaricus 20 parts, bacillus acidophilus 15 parts, Lactobacillus casei 10 parts, streptococcus thermophilus 10 parts, saccharomyces cerevisiae 10 parts, Candida utilis 6 parts；

Described probiotic bacteria powder probiotics viable bacteria content is: 8x10¹²cfu/g；

Preparation method, comprises the steps:

5) whole good granule mixes 8min with embedding probiotic bacteria powder, magnesium stearate, after mix homogeneously, fills grain, selects capsule, polishing, Make health-care edible capsule agent.

Embodiment 5

A kind of health food granule improving intestinal microbial population, is prepared by the raw material of following parts by weight:

D-mannital 5 parts, maltodextrin 5 parts, microcrystalline Cellulose 5 parts, 5 parts of probiotic bacteria powder, modified dietary fiber 5 Part, oligomeric xylose 3 parts, hydroxyl isomaltulose 3 parts, concentrated lactoalbumin 3 parts, fungi extracts 2 parts, Herba Saussureae Involueratae is cultivated Thing extract 3 parts, pectin decomposer 2 parts, cyclohexaamylose 1 part, citric acid 0.1 part, magnesium stearate 0.1 part, milk 0.1 part of essence, beta-carotene 0.01 part；

Preparation method, comprises the steps:

5) whole good granule mixes 8min with embedding probiotic bacteria powder, magnesium stearate, after mix homogeneously, packs, seals and i.e. obtain guarantor Health food granule.

Embodiment 6

A kind of health food powder improving intestinal microbial population, is prepared by the raw material of following parts by weight:

D-mannital 50 parts, maltodextrin 30 parts, microcrystalline Cellulose 30 parts, 30 parts of probiotic bacteria powder, modified meals are fine Tie up 20 parts, oligomeric xylose 15 parts, hydroxyl isomaltulose 15 parts, concentrated lactoalbumin 15 parts, fungi extracts 12 parts, Saussurea involucrata culture extract 10 parts, pectin decomposer 10 parts, cyclohexaamylose 10 parts, citric acid 3 parts, magnesium stearate 3 Part, milk flavour 3 parts, beta-carotene 3 parts；

Preparation method, comprises the steps:

1) press formula proportion, by modified dietary fiber, cyclohexaamylose and probiotic powder mixing 8min, obtain embedding probiotic powder Agent；

2) according to formula proportion, by D-mannital, maltodextrin, microcrystalline Cellulose, oligomeric xylose, hydroxyl isomaltulose, Concentrated lactoalbumin, fungi extracts, Saussurea involucrata culture extract, pectin decomposer, citric acid are pulverized, and cross 100 mesh sieves, Then mix 8min i.e. obtain health food powder with embedding probiotic bacteria powder, beta-carotene, milk flavour, magnesium stearate.

Embodiment 7 present invention can improve the sensory evaluation test of the health food of intestinal microbial population

The health food inviting 24 personnel to prepare embodiment of the present invention 2-6 is carried out with commercially available two kinds of health foods containing probiotic bacteria Judging, sense organ marking, wherein specialty and each 12 of layman, professional is young, middle aged, each 4 of old age, men and women Half and half, layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half；Marking includes outward appearance (20 points), matter Ground (25 points), local flavor (30 points), four aspects of mouthfeel (25 points), marking personnel independently carry out, are independent of each other, to protect It is accurate that card judges result.Being added up judging result, equal score value takes approximation, retains integer, is specifically shown in Table 1:

Table 1 sensory evaluation statistical result

Note: show significant difference (P ＜ 0.05) with a line internal standard difference lowercase alphabet, the different capitalization of mark represents that difference is extremely notable (P ＜ 0.01), Indicate same letter and represent that difference is not notable (P ＞ 0.05).

Result above shows, health food prepared by the present invention from outward appearance, quality, local flavor and mouthfeel any one will be the most excellent In the commercially available health food containing probiotic bacteria, particularly local flavor is fabulous, also is adapted for different age group, different hierarchy of consumption simultaneously Consumer eat.

Embodiment 8 present invention can improve the probiotic test of the health food of intestinal microbial population

The health caring food tablet embodiment of the present invention 2 prepared, being prepared as viable count with sterilized water is 2 × 10¹⁰CFU/mL's is prebiotic Bacterium solution, saves backup in 4 DEG C；

1, the 10mL probiotic solution kept of going bail for is injected in test tube 1, uses ten times of stepwise dilutions to 10^-8, take 1mL diluent On flat board, the MRS agar culture medium being cooled to 45 DEG C is poured on flat board (sterilizing), shake up rapidly after sterilizing.Again will dress The test tube 2 having 10mL probiotic solution is placed in 80-90 DEG C of water-bath heating 15-25min, takes the probiotic solution after heating and carries out Ten times of stepwise dilutions are to 10^-8, take 1mL diluent on flat board, the MRS agar culture medium being cooled to 45 DEG C poured into flat after sterilizing Plate (sterilizing) is upper and shakes up rapidly.Finally the flat board before heating and after heating is all cultivated under the conditions of 35 DEG C 24h, calculates heating Quantity front and back.

Result shows, Viable detection has reached 88%.

2, simulated gastric fluid and the resistance test of intestinal juice: the hydrochloric acid 16.4mL taking 100g/L adds distilled water diluting, makes pH value be respectively 1.5,2.5 and 3.5, take 100mL dilute hydrochloric acid solution, be separately added into 1g pepsin so that it is fully dissolve, obtain simulated gastric fluid, Microporous filter membrane degerming (0.22 μm) is standby.Taking potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, uses 0.1moL/L hydroxide Sodium solution regulation pH value is to 6.8；Separately taking trypsin 10g, the 100mL that adds water makes dissolving, after two liquid mixing, is diluted with water to 1000ml, obtains simulated intestinal fluid, and microporous filter membrane degerming (0.22 μm) is standby.Take the probiotic solution that 1mL keeps and join 9mL Simulated gastric fluid in (i.e. ten times stepwise dilutions), and the most fully mix rapidly, be subsequently placed in 30-45 DEG C of quiescent culture 2-4h.1h, 2h, 3h, 4h when, take out culture fluid respectively and count remaining viable count immediately, comparing with former viable count Relatively, result shows, Viable detection is 97%.Then each 1mL of culture fluid digesting different time it is taken in simulated gastric fluid, respectively Be inoculated in the simulated intestinal fluid that 9mL pH value is 6.8, be placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h takes Sample, measures its viable count, compares with former viable count, and result shows that Viable detection is 99%.

3, the resistance test of cholate is simulated: make the solution of 1g/L, and the Fel Sus domestica salt of addition 0.8% in the solution with pancreatin, It is 8.0 that NaOH with 10% adjusts pH, then with 0.45 μm micro-filtrate membrane filtration degerming.The probiotic solution that 0.5mL is kept It is inoculated in 4.5mL simulation cholate, after cultivating 24h, obtains culture fluid, the viable count of counting remaining.By culture fluid at sterile physiological In saline, ten times of stepwise dilutions are to 10^-8, and pour into MRS, it is subsequently placed in 35 DEG C of quiescent culture 24h.Result shows Viable detection It is 99%.

Above result of the test shows, the probiotic bacteria probiotic (thermostability, resistance to pH, bile tolerance) in health food of the present invention is relatively By force, being especially suitable for human intestines and stomach's environment, in simulated gastrointestinal environments, survival rate is big, can be effectively improved gastrointestinal function.

It should be understood that health food prepared by embodiment of the present invention 3-6 has above-mentioned experiment effect equally, each embodiment it Between and little with above-mentioned experiment effect diversity.

Embodiment 9 health food mouse intestinal performance test

Choosing common Kunming white mice 60, male and female half and half, 18-20g, conventional word is supported.Therefrom random choose 40, every day is early 9:00 gavage lincomycin hydrochloride 0.2mL in morning (20mg)/only, other as a control group, the gavage equivalent sterilizing of every day same time Normal saline, continuous one week, the mouse model of preparation alteration of intestinal flora.Model group mouse diet declines, do not occur dead and Significantly suffering from diarrhoea phenomenon, arrange soft excrement, profile normal aqueous divides more, and bedding and padding are moist.By 40 alteration of intestinal flora mices, with Machine is divided into 2 groups, and one group 20 is only used as treatment group, the probiotic solution 0.5ml (2 × 10 that every day, gavage was kept¹⁰cfu/ml)/ Only, another 20 are only used as natural recovering group, every day same time gavage equivalent sterile saline, continuous two weeks.Whole experimental period 21 days, observe growth and the defecation situation of white mice every day, in the 8th, 21 days to probiotic tablet treatment group and natural recovering group Mice is weighed, and calculates each group of weight average rate of increase, result such as table 1；Within every 5 days, survey each group of stool in mice escherichia coli quantity, Calculate average, result such as table 2.Take stool in mice about 0.1g, in aseptic operating platform, add 3 beades (with 0.1g excrement sample Add 0.5mL diluent), dilute and inoculate maconkey agar culture medium, calculate every gram wet just in coliform count.

Table 2 mice Gain weight

Packet	Average starting weight (g/ is only)	Average end weight (g/ is only)	Average rate of increase (%)
				Natural recovering group	20.69±1.33	27.34±1.59	32.14^a
Treatment group	20.41±1.45	32.58±1.62	59.63^b

The situation of coliform count in table 3 stool in mice

Health food treatment group Mouse Weight average rate of increase (59.63%) is significantly higher than natural recovering group (32.14%)；Feed Solution rear intestinal escherichia coli quantity is remarkably decreased, reduction by 82.62%, and substantially less than natural recovering group (24.78%) shows this Probiotic bacteria in invention health caring food tablet is rapid field planting in white mice intestinal, forms dominant microflora, and effectively suppression large intestine bar The growth and breeding of the pathogen such as bacterium, and resident time is long, continues, effectively improves intestinal performance.

The impact on immunity of organisms of embodiment 10 health food of the present invention

1 experiment purpose

Test (mouse forced swimming) by exercise tolerance, verify the raising immunity of health food of the present invention, antifatigue effect.

2 experiment materials and reagent

2.1 for reagent thing:

The commercially available health food (G1) containing probiotic bacteria；The commercially available health food (G2) containing probiotic bacteria；Embodiment of the present invention 2-6 system Standby health food (G3-G7).

2.2 reagent:

Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing；Concentrated sulphuric acid (AR), the limited public affairs of Nanjing chemical reagent Department；Normal saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..

3. laboratory animal

ICR mice, ♂, cleaning grade, body weight 18-22g, Yangzhou University's comparative medicine center provide, the quality certification number: SCXK (Soviet Union) 2,007 0001, the free diet of mice during experiment.

4. key instrument

Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof is public Department；Water-bath: DK-S26 type, upper Nereid grand experimental facilities company limited；Electronic scale: BS224S type, Sartorius Company；Stopwatch, thermometer

5. experiment packet

5.1 dosage packets and given the test agent give the time and at random mice are divided into 8 groups, and often group 10, the 1st group to the 7th component Not Gei the medicine of G1～G7, the 8th group is blank group, gives isopyknic distilled water, the often every average daily gavage of group 1 time, Gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.

5.2 sample preparations the 1st group are to the 7th group: weigh 2.25g drug sample, be assigned to 150ml with distilled water；Blank Group: distilled water 150ml.

6. experimental technique

6.1 swimmings with a load attached to the body experiment lasts are administered after 30min, put mice in swimming trunk, and the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of rat-tail root load 5% body weight, record mice swimming starts to the dead time, when swimming as mice Between.

After 6.2 mice serum carbamide measure last administration 30min, not swimming with a load attached to the body 90min in the water that temperature is 30 DEG C, Pluck after rest 60min eyeball blood sampling 0.5mL (being not added with anticoagulant), put 4 DEG C of refrigerator 3h, after hemopexis 2000r/min from Heart 15min, takes serum and send clinical laboratory of Lianyungang First People's Hospital to detect.

After the mensuration last of 6.3 hepatic glycogen is administered 30min, not swimming with a load attached to the body 90min in the water that temperature is 25 ± 1 DEG C, cervical vertebra Mice is put to death in dislocation, cleans with normal saline, and with after filter paper suck dry moisture, accurately weighs liver 100mg, and hepatic glycogen detects Test kit detection Mouse Liver glycogen content.

The mensuration last of 6.4 blood lactase acid is taken a blood sample after being administered 30min, does not the most bear a heavy burden at the water went swimming 10min that temperature is 30 DEG C Rear stopping.Lactic acid instrument assay method: respectively before swimming, each blood sampling 20 μ L add after rest 20min after swimming, after swimming Entering in 40 μ L rupture of membranes liquid, the most fully vibration smudge cells lactic acid instrument measures.(blood lactase acid area under curve=5 × (swimming The blood lactase acid value of 20min after blood lactase acid value+2 × swimming of 0min after front blood lactase acid value+3 × swimming)

7. observation index walking weight load, blood lactase acid, carbamide, glycogen initial value

8. statistical method experimental data x ± s represents, uses t inspection to compare between organizing

9. experimental result

The impact on Mouse Weight of 9.1 health foods of the present invention

Each group mice after giving G1～G7 medicine, front, in, shown in post-weight see table respectively, at the beginning of each group mice Initial body weight and weightening finish body weight compare equal no difference of science of statistics (P ＞ 0.05) with matched group, show that G1～G7 medicine is all without significantly Toxicity.Experimental result refers to table 4.

The original body mass of table 4 swimming with a load attached to the body experiment mice, body weight in mid-term and end body weight

The impact on the mice burden swimming time of 9.2 health foods of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, can be obviously prolonged mice and bear Weight swimming time, has significant difference (P ＜ 0.05), and health food G3 of the present invention～G7 medicine compare with blank group, Can significantly extend the mice burden swimming time, there is pole significant difference (P ＜ 0.01), and be substantially better than G1～G2 medicine. The results detailed in Table 5.

The impact on the mice burden swimming time of table 5 health food of the present invention

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

9.3 health foods of the present invention are on the impact of blood lactase acid before and after mouse movement

After per os gives mice health food of the present invention, health food G3 of the present invention～G7 medicine are bent to blood lactase acid after mouse movement Under line, area compares with matched group significant difference (P ＜ 0.05), G1～G2 medicine group Mouse Blood lactic acid area under curve with Decrease though matched group compares, but and no difference of science of statistics (P ＞ 0.05).The results are shown in Table 6.

Table 6 health food of the present invention is on the impact of blood lactase acid level before and after mouse movement

" * " p < 0.05vs blank；

The impact on Mouse Liver glycogen of 9.4 health foods of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, and Mouse Liver glycogen content all has bright Aobvious rising, have significant difference (P ＜ 0.05), health food G3 of the present invention～G7 medicine with blank group ratio Relatively, Mouse Liver glycogen content all has significantly rising, has pole significant difference (P ＜ 0.01), and is substantially better than G1～G2 Medicine.The results detailed in Table 7.

The impact on Mouse Liver glycogen content of table 7 health food of the present invention

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

The impact on mice serum carbamide of 9.5 health foods of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine group compares with blank group, serum urea after mouse movement Content all has significantly reduction, have significant difference (P ＜ 0.05), health food G3 of the present invention～G7 medicine with blank Matched group compares, and after mouse movement, serum urea content all has obvious reduction all to have significantly reduction, has pole significant difference (P ＜ 0.01), and it is substantially better than G1～G2 medicine.The results detailed in Table 8.

The impact on mice serum urea content of table 8 health food of the present invention

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

10. experiment conclusion

This experiment is mainly tested by mice burden swimming, and the deposit of detection Mouse Liver glycogen observes health food of the present invention simultaneously Improve immunity, resisting fatigue effect.Preliminary Results shows as follows:

1, G3～G7 health food of the present invention all can extend the mice burden swimming time (P ＜ 0.01), and effect is substantially better than The analysis of functional food sold of other G1～G2.

2, biochemistry detection aspect shows, G3～G7 health food of the present invention each dosage group all can reduce move after Portugal in mice serum Lactic acid content produced by grape sugar anerobic glycolysis, compares with matched group and has significant difference (P ＜ 0.05), and other G1～G2 Although analysis of functional food sold also can reduce after motion lactic acid content produced by glucose anerobic glycolysis in mice serum, but with right Compare according to group, no difference of science of statistics (P ＞ 0.05)；

3, G3～G7 health food of the present invention each dosage group all can significantly improve the deposit (P of glycogen in mouse liver ＜ 0.01), and effect is substantially better than the analysis of functional food sold of other G1～G2；

4, metabolic arthritis model finds, G3～G7 health food of the present invention can significantly reduce the content of urea in serum after mice is swum (P ＜ 0.01), and effect is substantially better than other G1～G2 analysis of functional food sold；

11. conclusions

Above-mentioned experiment proves that health food of the present invention can significantly improve immunity of organisms, improves muscle power and the endurance of mice, reduces mice Urea in serum and the content of lactic acid after motion, and the deposit of glycogen in mouse liver can be significantly improved, contribute to alleviating motion negative The fatigue that lotus causes；The time that mice burden swimming to power exhausts can be extended.

Claims

1. the preparation method of the health food that can improve intestinal microbial population, it is characterised in that described health food is by following parts by weight Raw material prepare: D-mannital 5-50 part, maltodextrin 5-30 part, microcrystalline Cellulose 5-30 part, probiotic bacteria powder 5-30 Part, modified dietary fiber 5-20 part, oligomeric xylose 3-15 part, hydroxyl isomaltulose 3-15 part, concentrated lactoalbumin 3-15 Part, fungi extracts 2-12 part, Saussurea involucrata culture extract 3-10 part, pectin decomposer 2-10 part, cyclohexaamylose 1-10 part, citric acid 0.1-3 part, magnesium stearate 0.1-3 part, milk flavour 0.1-3 part, beta-carotene 0.01-3 part；

Described probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 40-50 part, long bifid bar Bacterium 30-45 part, bifidobacterium bifidum 25-35 part, bifidobacterium adolescentis 15-20 part, Lactobacillus bulgaricus 15-20 part, Bacillus acidophilus's 10-15 part, lactobacillus casei 8-10 part, streptococcus thermophilus 8-10 part, saccharomyces cerevisiae 8-10 part, produce protein false Silk yeast 4-6 part；Lactobacillus plantarum powder is to be prepared by Lactobacillus plantarum tlj-2014, and this bacterial strain is in July, 2014 Within 2nd, being preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC NO.9405, point Class is named: Lactobacillus plantarum Lactobacillus plantarum；

The preparation method of described modified dietary fiber comprises the following steps: by inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber 8-10:4-6:3-5:2-4 in mass ratio uniformly mixes, and adds the water of its quality 3-7 times, room temperature 200-300W, 35-40KHz Condition supersound extraction 10-15min, then in electric field intensity 20-40kV/cm, burst length 400-500 μ s, pulse frequency High-pressure pulse electric extraction is carried out under the conditions of 200-300Hz；It is 4.5-6.5 with breast acid for adjusting pH value, adds mixture quality The enzyme of 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Enzymolysis solution concentrating under reduced pressure, lyophilization, low-temperature grinding are to grain Footpath is that 0.1-0.3mm i.e. obtains modified dietary fiber；Described enzyme is xylanase, cellulase, laccase, pectase, list Peaceful enzyme 5-9:2-4:2-4:2-4:1-3 in mass ratio uniformly mixes；

The preparation method of described fungi extracts comprise the steps: to be respectively put into Hericium erinaceus (Bull. Ex Fr.) Pers., Semen Ginkgo and Auricularia equipped with The ultrasonic washing unit of 0.3-0.5% sodium bicarbonate solution cleans 5-10min in 200W, 40KHz, drains, be cut into equal Specification, length and width is 5-10mm, and the sheet of thickness 3-5mm or fourth, in mass ratio 8-10:5-7:4-6 uniformly mix, then in -21-25 DEG C of freezing 10-30min, pulverize immediately, and ground product particle diameter is 0.2-1mm；Add ground product quality 3-5 times Water obtain mixture, adjusting mixture pH is 4.5-6, in electric field intensity 35-45kV/cm under room temperature, burst length 500-700 μ s, Carry out high voltage pulse electric field processing under the conditions of pulse frequency 200-400Hz, be warming up to 45～55 DEG C, add mixture weight The mixed enzyme enzymolysis 4 of 0.5-1.2%～6 hours, obtain edible fungi enzymolysis solution, and being evaporated to solid content is more than 30%, Lyophilization, low-temperature grinding to particle diameter are that 0.1-0.3mm i.e. obtains fungi extracts；Described mixed enzyme parts by weight consist of: Papain 20-30 part, bromelain 20-30 part, glucanase 10-15 part, xylanase 10-15 part, penta gathers Carbohydrase 10-15 part, ficin 5-10 part, midrange thermal stable amylase 5-10 part, pectase 5-10 part, tannase 3-5 part；

The preparation method of described Saussurea involucrata culture extract comprises the steps: to take Saussurea involucrata culture, immerses and fills mass percent Concentration is in the container of aseptic oligofructose aqueous solution of 0.9-1.1%, takes out, then in-18-23 DEG C of freezing 5-10min； It is crushed to particle diameter 0.3-1mm immediately；Put in container and add the water of 3-5 times of weight or the ethanol of 35-75%, obtaining mixed material, It is 3.5-5.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 25-35kV/cm, burst length 300-500 μ s, pulse High voltage pulse electric field processing is carried out under the conditions of frequency 200-300Hz；Then heat to 30-40 DEG C, insulation, power 150-300W, Carry out microwave extraction, wherein, each microwave exposure total time 60-80s under the conditions of frequency 2000Hz, carry out compartment irradiation: spoke According to 10s, it is spaced 10s, such irradiation 10 times, simultaneously at power 200-300W, carries out ultrasonic under the conditions of frequency 30-40KHz Ripple assisted extraction；It is continuously heating to 40-50 DEG C, insulation, it is subsequently adding the compound enzyme enzymolysis of mixed material gross weight 2.5-4% 30-50min, filters, obtains filtrate；Filtering residue rinses 3 times with 1-3 times of weight 76-80 DEG C water, and rinsing liquid merges with filtrate, all Even mixing, at room temperature in electric field intensity 35-45kV/cm, burst length 400-600 μ s, under the conditions of pulse frequency 200-300Hz Carry out high voltage pulse electric field processing and i.e. obtain Saussurea involucrata culture extract；Described compound enzyme by pectase, cellulase, tannase, Amylase and protease 3-7:3-5:2-4:1-3:1-2 in mass ratio uniformly mixes；

The preparation method of described pectin decomposer comprises the steps: fresh Fructus Crataegi marc, Pericarpium Citri grandis, pomace, megasse It is respectively put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and cleans 5-10min in 200W, 40KHz, drain, cut Being divided into phase equal-specification, length and width is 5-10mm, and the sheet of thickness 3-5mm or fourth, in mass ratio 8-12:3-5:2-4:2-4 are uniform Mixing, then in-21-25 DEG C of freezing 10-30min；Pulverizing immediately, ground product particle diameter is 0.1-1mm；Add and pulverize The deionized water of material amount 3-5 times, mix homogeneously, is 2-3 with Fructus Citri Limoniae acid for adjusting pH value, in electric field intensity under room temperature 35-45kV/cm, burst length 500-700 μ s, carry out high voltage pulse electric field processing, then under the conditions of pulse frequency 200-400Hz Normal pressure boils 30-50min, Bag filter, and filtering residue boils 40-60min with the filtrate normal pressure of its quality 1-3 times, Bag filter, Merging filtrate, kieselguhr filters, makes filtrate clarify, and being evaporated to solid content at 75 DEG C is 6-10%, cools down rapidly To room temperature, add 95% ethanol that pH value is 2-3 of 1.5 times of volumes of concentrated solution, stand 20-30min, make pectin be precipitated out, 4000r/min is centrifuged 5-10min, reclaims precipitation, with the dehydrated alcohol eluting of 2 times of volumes, centrifugal, reclaims precipitation, so grasps Making 2 times, obtain is deposited in 40-60 DEG C of vacuum drying 2-3h, it is thus achieved that pectin, adds 40-50 DEG C of water of pectin quality 4-5 times, Stirring, uniformly mixes, insulation, is 4.5-5.5 with breast acid for adjusting pH value, adds the pectinase enzymatic hydrolysis 2-4h of 0.3-0.8U/mL, Enzymolysis solution filters, and filtrate is enzyme denaturing 10-15min in boiling water, is cooled to room temperature, and ultrafiltration concentration, lyophilization i.e. obtain pectin Thing；

The preparation method of described health food comprises the steps:

4) by modified dietary fiber, cyclohexaamylose and probiotic bacteria powder mixing 5-10min, probiotic bacteria powder must be embedded；

2. the preparation method of the health food that can improve intestinal microbial population, it is characterised in that described health food is by following parts by weight Raw material prepare: D-mannital 5-50 part, maltodextrin 5-30 part, microcrystalline Cellulose 5-30 part, probiotic bacteria powder 5-30 Part, modified dietary fiber 5-20 part, oligomeric xylose 3-15 part, hydroxyl isomaltulose 3-15 part, concentrated lactoalbumin 3-15 Part, fungi extracts 2-12 part, Saussurea involucrata culture extract 3-10 part, pectin decomposer 2-10 part, cyclohexaamylose 1-10 part, citric acid 0.1-3 part, magnesium stearate 0.1-3 part, milk flavour 0.1-3 part, beta-carotene 0.01-3 part；

The preparation method of described health food comprises the steps:

1) press formula proportion, by modified dietary fiber, cyclohexaamylose and probiotic bacteria powder mixing 5-10min, must embed prebiotic Bacteria powder；

3. can improve the preparation method of the health food of intestinal microbial population as described in claim 1-2 is arbitrary, it is characterised in that health food Prepared by the raw material of following parts by weight: D-mannital 15-35 part, maltodextrin 10-20 part, microcrystalline Cellulose 10-20 Part, probiotic bacteria powder 10-20 part, modified dietary fiber 8-12 part, oligomeric xylose 7-11 part, hydroxyl isomaltulose 7-11 part, Concentrated lactoalbumin 7-11 part, fungi extracts 5-10 part, Saussurea involucrata culture extract 5-8 part, pectin decomposer 5-7 Part, cyclohexaamylose 1.5-5 part, citric acid 1.5-2.5 part, magnesium stearate 1.5-2.5 part, milk flavour 1.5-2.5 part, Beta-carotene 1-2 part.

4. can improve the preparation method of the health food of intestinal microbial population as described in claim 1-2 is arbitrary, it is characterised in that health food Prepared by the raw material of following parts by weight: D-mannital 25 parts, maltodextrin 15 parts, microcrystalline Cellulose 15 parts, probiotic bacteria 15 parts of powder, modified dietary fiber 10 parts, oligomeric xylose 9 parts, hydroxyl isomaltulose 9 parts, concentrated lactoalbumin 9 parts, Fungi extracts 8 parts, Saussurea involucrata culture extract 7 parts, pectin decomposer 6 parts, cyclohexaamylose 3 parts, citric acid 2 Part, magnesium stearate 2 parts, milk flavour 2 parts, beta-carotene 1.5 parts.