CN105831666A

CN105831666A - Probiotic honey composition and preparation method thereof

Info

Publication number: CN105831666A
Application number: CN201610178652.3A
Authority: CN
Inventors: 李建树
Original assignee: Tianjin Zhongtian Jingke Technology Co Ltd
Current assignee: Tianjin Zhongtian Jingke Technology Co Ltd
Priority date: 2016-03-25
Filing date: 2016-03-25
Publication date: 2016-08-10

Abstract

The invention discloses a probiotic honey composition. The probiotic honey composition is prepared with honey as a main raw material and through a step of employing a part of the honey, a malt extract, leek flowers, and a probiotic powder as raw materials, and performing low-temperature enzymolysis, high-voltage pulse electric field sterilization, temperature-varying fermentation, probiotic inoculation in batches, ultra-filtration and concentration to the raw materials to prepare a honey probiotic fermentation concentrate liquid which has a moderate sour-and-sweet taste and is high in contents of probiotics and functional metabolites thereof, wherein the fermentation concentrate liquid can reach 8.12*10<12> - 9.58*10<12> CFU/ml in probiotic content and has very excellent probiotic property, and finally the fermentation concentrate liquid is mixed with the rest of the honey, the malt extract, raw ginger extract and a functional auxiliary to prepare the probiotic honey composition which is high in honey content, has a moderated sour-and-sweet taste, is free of crystallization even being stored for a long time (36-48 months), and has significant health caring effects.

Description

A kind of probiotic bacteria honey composition and preparation method thereof

Technical field

The present invention relates to bee product, particularly to a kind of probiotic bacteria honey composition and preparation method thereof.

Background technology

Mel is a kind of preferably natural health-care products that Apis herborization nectar leads to.Its main component has: (1) glucose, really Sugar: both sugar accounts for more than 65% in Mel.It gives the sweet taste of Mel, hygroscopicity and the value of energy and tangible feature. (2) acids: the acids in Mel includes organic acid, mineral acid and aminoacid.Organic acid is mainly gluconic acid and citric acid. Mineral acid includes phosphoric acid, hydrochloric acid, boric acid and carbonic acid.About 17 kinds of aminoacid in Mel, wherein proline is main Aminoacid, next to that lysine and aspartic acid.(3) enzyme: mainly invertase and amylase.(4) vitamin: Mel In vitamin the abundantest with B race, next to that vitamin C.Common vitamin have riboflavin, pyrrole polyacid (VB6), nicotinic acid, Folic acid, ascorbic acid.Also have biotin, tocopherol etc..Vitamin in Mel, essentially from the pollen in Mel, comes on a small quantity From nectar.(5) mineral: the content of mineral substances in Mel is widely different, has substantial connection with the soil of plant growing.In Mel Content of mineral substances and kind and the content in blood of human body, kind the most close, and the content of mineral substances ratio in dark Mel Light color Mel is high.Mel Jujubae belongs to dark honey, and its mineral contained, trace element are above other Mel after measured.As potassium, Sodium, calcium, other Mel of phosphorus geometric ratio exceed 4～10 times.Ferrum, copper, selenium are high about 10 times.(6) protein: the egg in Mel White matter has 4～7 kind, and wherein 3 kinds from nectar, separately has 4 kinds from Apis.Containing a kind of colloidal substance in Mel, it be by Protein, wax class, pentosan and inorganic matter composition, in dark Mel, content is about 1%.It is to the color and luster of Mel and muddiness Degree has an impact.(7) aromatic substance: the aromatic substance in Mel is mainly the derivant of alcohols and aldehydes and corresponding lipoid substance thereof. These aromatic substance major parts are from nectar, and small part produces in Mel brewing process, and it gives the fragrance that Mel is unique. (8) other material: possibly together with pigment, pollen, sugar alcohols, tannin class, resin and other bioactive substance in Mel.

Processed with honey cream is a kind of unguentum prepared for primary raw material is equipped with other functional food or pharmaceutical raw material with Mel, at present, The kind of processed with honey cream is more, and Chinese patent CN 102334626 B discloses unguentum goods and the preparation of a kind of bee product compound prescription Method, with health role with Mel, broker wall bee pollen, fresh royal jelly, queen bee nit lyophilized powder, drone pupa freeze-dried powder five kinds Raw material prepare, the invention discloses the formula of five-apian treasure cream, composition is by weight, be respectively Mel 60-85%, Broker wall bee pollen 10-15%, fresh royal jelly 3-10%, queen bee nit lyophilized powder 0.5-2%, drone pupa freeze-dried powder 0.5-2%. The invention also discloses the preparation method of this five-apian treasure cream, preparation method is that moisture Control is pre-at the Mel 60 DEG C of 19-22% Heat, and open agitating device, fresh royal jelly, broker wall bee pollen, queen bee nit lyophilized powder, drone pupa freeze-dried powder etc. are joined honeybee In honey, mix homogeneously, after colloid mill homogenizing, bottling.So polytrophic bee product is gathered together, give full play to For supplementing the nutrients, improve the effect of body constitution, the most also this product can be diluted or be directly used in face nourishing and whitening and moisten.

Chinese patent CN 103519041 B vegetables and fruits rice juice honey paste and preparation method thereof, it is characterised in that by following weight The raw material of part is made: Mel 50-55, high fructose syrup 10-12, taro meal 4-5, sweet potato powder 12-14, rice 5-7, Hu Luo Foretell 12-15, Fructus Cucurbitae moschatae 10-13, Herba Apii graveolentis 20-30, Fructus Crataegi 2-3, Herba Lophatheri 3-4, Herba Commelinae Bengalensis 4-5, flower of Radix Notoginseng 1-2, Yellow leaf 3-4, Folium Myricae rubrae 4-5, chestnut shell 20-12, leaf of Cerasus humilis Bunge 4-5, water are appropriate.Mel tonifying the spleen and stomach in the present invention, intestine moistening Road, skin care；Vegetable melon and fruit can supplement vitamin and the trace element of needed by human body；Rice, taro meal, sweet potato powder are mended Fill carbohydrate；Chinese medicine extract adds health-care effect, it is possible to heat-clearing and toxic substances removing, nourishing the liver and kidney；And taste is mellow, mouth Sense uniqueness.

The processing method that Chinese patent CN 103039774 B discloses a kind of compound momordica grosvenori syrup.By Fructus Momordicae, Bulbus Lilii, river Shellfish and Radix Glycyrrhizae add water clean, add water boil and extract 3 times, and united extraction liquid filters, and filtrate is concentrated into fluid extract；By fluid extract Add in Mel and mix, heated and boiled, bottle while hot, seal, obtain compound momordica grosvenori syrup.Product of the present invention have lung moistening, Removing heat from the lung and relieving sorethroat, effect of preventing phlegm from forming and stopping coughing.It is suitable for the person that need to protect phonatory organ such as performer, teacher, broadcaster, shop assistant, chronic Pharyngitis patient.Add Fructus Momordicae intensive processing product variety, beneficially industrialized production.

Chinese patent CN 101869235 B discloses formula and the preparation method of a kind of nutrient honey goods, uses the most exactly There are a few herbs of nutrition health-care functions and be prepared by Mel, the invention discloses the formula of Chinese medicine, including Semen Cassiae, Fu Siberian cocklebur, Fructus Lycii, Flos Chrysanthemi, Semen Ginkgo, Semen Pruni, Fructus Cannabis, Folium Perillae, Radix Glycyrrhizae.Composition is by weight, goods contain 3.0%～ The Chinese medicine extract of 5.0%, containing the Mel of 95%～97%.The invention also discloses the preparation method of these goods, preparation side Method is to extract after Semen Cassiae, Poria, Fructus Lycii, Flos Chrysanthemi, Semen Ginkgo, Semen Pruni, Fructus Cannabis, Folium Perillae, Radix Glycyrrhizae mixing, carries Take after liquid concentrates and be added in Mel, and by the two mix homogeneously, reconcentration, bottling.During taking bee product permissible The effective ingredient of above-mentioned a few herbs is taken in internal, has both decreased the trouble taking these a few herbs, also mitigate different medicine The taste stimulation to human body, enhances again the health-care effect of Mel and Chinese medicine simultaneously.

Chinese patent CN 104757363 A discloses one and relieves the effect of alcohol honey paste, belongs to food technology manufacture field, including following weight Each component of amount number: Fructus Jujubae 4-8 part；Fructus Lycii 4-8 part；Rhizoma Zingiberis Recens 5-10 part；Pericarpium Citri tangerinae 5-10 part；Vitamin C 1-3 part； Vitamin E 1-3 part；Olive oil 2-5 part；Vinegar 10-15 part；Mel 50-70 part.Present invention also offers above-mentioned relieving the effect of alcohol The preparation method of honey paste and using method.The present invention makes drunk people relieving alcoholic intoxication at short notice, alleviates and releases and draws because of drunk Uncomfortable and the painful sensations risen, solves the problem that effect that simple vinegar carries out relieving the effect of alcohol is limited, simple and convenient, existing solution Wine effect, nourishing the liver can protect stomach again, drinks conveniently.

Chinese patent CN 105192506 A discloses the processing method of a kind of Cornu Cervi Pantotrichum honey paste.Mainly for solving to take deer at present The fine and soft problem that can produce the untoward reaction such as scorching or diarrhoea with Mel blended product and invent.It is by such as by following raw material Lower weight portion prepares: Mel 80～90 parts, enzymolysis Pulveratum Cornu Cervi Pantotrichum 8～18 parts, Cortex Phellodendri extract 2 parts；Mel is by percentage by weight It it is the Mel composition of the acacia honey of 85%, the osmanthus honey of 10% and 5%；Concrete preparation method is: by acacia honey, osmanthus honey, Mel is mixed in rustless steel container and is placed in water heating, heats 55 DEG C and melts in advance, adds after it has preferable mobility Enzymolysis Pulveratum Cornu Cervi Pantotrichum and Cortex Phellodendri extract, stir while adding, and 12min is after it fully mixes in stirring, crosses colloid mill and grinds 5min, Cross 180 mesh sieves, enter sterilization tank, 81 DEG C of sterilizing 30min.Advantage is Mel and Cornu Cervi Pantotrichum is played that fall is dry, the effect of convergence by Cortex Phellodendri.

Chinese patent CN 103637218 B discloses a kind of health-care honey and preparation method thereof, and raw material consists of: black honeybee Mel 100 Part, Poria 5-15 part, Radix Astragali 5-15 part, Fructus Jujubae 5-15 part.The present invention is directed to Mel single product health-care effect onset slow Deficiency, according to experience for many years and substantial amounts of experiment screening, Multiple components is added in black honeybee Mel according to specific ratio, Obtain health-care honey of the present invention.Health-care honey of the present invention is nutritious, has defying age, enhancing immunity, building body, taste Profit effect such as skin care, loosening bowel to relieve constipation, is particularly suitable for having a delicate constitution, constipation crowd, can quickly nurse one's health body constitution, make physical recovery be good for Health state.The method preparation of the present invention is simple, it is easy to accomplish, it is simple to industrialized production.

Honey paste disclosed above, all with Mel as primary raw material, adds functional raw material, to solve corresponding functional issues, But, quality is preferable, and the processed with honey cream that honey content is higher, moisture is relatively low is along with the difference of ambient temperature, it may appear that different Degree local-crystalized, although do not affect the inherent quality of processed with honey cream, but crystallization that also will not be overall, homogeneous as pure Mel, There will be inhomogenous crystallization and affect presentation quality, the quality mind misguided the consumer, reduces desire to buy and the appetite of client； Meanwhile, the purest Mel or processed with honey cream long-term drink there will be the situation such as flatulence, gastric acid, individual other there will be that " Mel is not Indication ", situation is more serious；And because of in Mel containing special organic acid, mineral etc., mouthfeel is sour and astringent, saccharic acid ratio Imbalance, the inapplicable vast consumer group.Chinese patent CN 104757357 A discloses the processing method preventing honey crystallization, Belong to food processing technology field.The method, in honey in bottle traditional processing technology step, is simultaneously introduced during concentrating Mel Melting the link of the nuclei of crystallization, its operational approach is: when concentrating Mel and starting, first Mel temperature is increased to 77 DEG C, is incubated 5 Minute, it is then switched off intake valve and concentrates, when Mel temperature drops to 50-60 DEG C, control to continue in this temperature range dense Contracting, to the water content conformance with standard of honey, then pumps into honey the outer storage honey jar having heat-insulation layer, carries out ultrasonic Treatment 30 seconds, i.e. Destroy the nuclei of crystallization of Mel, again crystallize after preventing Mel cooling.The present invention installs insulation storage honey jar the most on a production line, Mel tentatively destroys the nuclei of crystallization, then ultrasonic Treatment in storage honey jar through cold-heat treatment in concentration step, thoroughly destroys the nuclei of crystallization, Obtained Mel not only flavour is good, and maintains original transparent liquid state, than cost used by general heating solution crystallization Low.The Mel that above-mentioned processing method obtains there is also when the temperature decreases there is crystalline polamer, has reversibility, and technique Complexity, cost is high.Chinese patent CN 101785539 B mono-kind is difficult to crystallized honey and preparation method thereof.By Mel is added The technological process of work and technological parameter are adjusted and optimize, through melting honey, coarse filtration, dispensing, middle filter, vacuum dehydration, broken crystalline substance Core, fine straining, cooling, fill, vanning operation obtain being difficult to crystallized honey.The present invention is compared with prior art, it is achieved that do not add Add any material and delay honey crystallization, extend the shelf life of Mel.Above-mentioned preparation method complex process, takes multistage filtering, Mel active constituent content reduces, and still can produce through crystallization in 12 months.

Therefore, a kind of honey content of preparation is high, sweet mouthfeel preservation suitable, long-term does not crystallizes, the significant probiotic bacteria of health-care effect Honey composition is necessary.

Summary of the invention

Solved by the invention technical problem is that the defect overcoming existing processed with honey cream, with Mel as primary raw material, science is compounding can be carried For comprehensive, natural plant enzyme and the Fructus Hordei Germinatus extract of multiple nutrients material；Can effectively prevent the crystallization of processed with honey cream to improve outside processed with honey cream Appearance quality and stability, the Rhizoma Zingiberis Recens extract of appetite strengthening；Can organic regulation human body overall immunity, enhancing macrophage phagocytic Ability, suppression harmful bacteria adhere at human body intestinal canal and attack to strengthen probiotic probiotic powder；Mel can be promoted to digest and assimilate, Preventing the fragrant-flowered garlic flower etc. of flatulence, as the main raw material of preparing of processed with honey cream, science is compounding, and a small amount of one or more are functional auxiliary Material, prepares that a kind of honey content is high, sweet mouthfeel preservation suitable, long-term does not crystallizes, health-care effect significant probiotic bacteria Mel Compositions.

In order to achieve the above object, the present invention is by the following technical solutions:

A kind of probiotic bacteria honey composition, is mainly prepared by the raw material of following parts by weight:

Mel 155-185 part, Fructus Hordei Germinatus extract 4-10 part, Rhizoma Zingiberis Recens extract 2-6 part, probiotic bacteria powder 1-3 part, fragrant-flowered garlic flower 1-3 Part；

Preferably, described probiotic bacteria honey composition, mainly prepared by the raw material of following parts by weight:

Mel 165-175 part, Fructus Hordei Germinatus extract 6-8 part, Rhizoma Zingiberis Recens extract 3-5 part, probiotic bacteria powder 1.5-2.5 part, fragrant-flowered garlic Flower 1.5-2.5 part；

It is highly preferred that described probiotic bacteria honey composition, mainly prepared by the raw material of following parts by weight:

Mel 170 parts, Fructus Hordei Germinatus extract 7 parts, Rhizoma Zingiberis Recens extract 4 parts, 2 parts of probiotic bacteria powder, fragrant-flowered garlic spends 2 parts；

Further, described probiotic bacteria honey composition also includes one or more adjuvants of following parts by weight:

Fruit powder 0.6-1.2 part, algae 0.5-1 part, aloe vera gel 0.5-1 part, bee pollen 0.2-0.8 part, low Xylan 0.2-0.8 part, oligochitosan 0.1-0.7 part, marine fishbone collagen oligopeptide powder 0.1-0.7 part, Chinese herbal medicine extract 0.1-0.6 part, Cordyceps militaris (L.) Link. 0.1-0.5 part；

Preferably, described probiotic bacteria honey composition also includes one or more adjuvants of following parts by weight:

Fruit powder 0.8-1.0 part, algae 0.7-0.9 part, aloe vera gel 0.7-0.9 part, bee pollen 0.4-0.6 part, Oligomeric xylose 0.4-0.6 part, oligochitosan 0.3-0.5 part, marine fishbone collagen oligopeptide powder 0.3-0.5 part, Chinese herbal medicine extracting Thing 0.2-0.4 part, Cordyceps militaris (L.) Link. 0.2-0.4 part；

It is highly preferred that described probiotic bacteria honey composition also includes one or more adjuvants of following parts by weight:

Fruit powder 0.9 part, 0.8 part of algae, aloe vera gel 0.8 part, bee pollen 0.5 part, oligomeric xylose 0.5 part, Oligochitosan 0.4 part, marine fishbone collagen oligopeptide powder 0.4 part, Chinese herbal medicine extract 0.3 part, Cordyceps militaris (L.) Link. 0.3 part；

Further, described Mel at least contains the Flos Robiniae Pseudoacaciae honey of 80-100%；

Further, described Fructus Hordei Germinatus extract does not contain only abundant plant rennet, amylase, hemicellulase, esterase, The various plants enzymes such as oxidoreductase and containing the nutrient substance such as vegetable polysaccharides and monosaccharide, plant amylum, vegetable protein, not only Can be that probiotic bacteria honey composition provides comprehensive, natural phytoenzyme, can be also that probiotic bacteria provides nutrient substance comprehensive, abundant；

Preferably, described Fructus Hordei Germinatus extract uses ultrasonic cleaning, microwave-assisted supersound extraction and high-pressure pulse electric to extract, reduces pressure The extract at low temperature technology such as concentration, are effectively increased raw material availability, phytoenzyme activity and productivity；Fructus Hordei Germinatus extract has been effectively ensured Foodsafety；

It is highly preferred that the preparation method of described Fructus Hordei Germinatus extract is: Fructus Hordei Germinatus and Fructus Tritici aestivi 8-10:1-3 in mass ratio are uniformly mixed Close, be crushed to granularity 0.5-1mm, obtain pulverizing Fructus Hordei Germinatus；Then by Fructus Chaenomelis, Fructus Ananadis comosi, Fructus Fici in ultrasonic washing unit Under the conditions of power 200W, frequency 30KHz, ultrasonic cleaning 5-10min, drains, and is crushed to granularity 0.5-1mm, and presses under room temperature Mass ratio 7-9:1-3:1-2 uniformly mixes, and the pulverizing Fructus Hordei Germinatus adding mixture quality 3-5 times obtains raw mixture, adds raw material The water of mixture quality 1-3 times, is 3-4 with Fructus Citri Limoniae acid for adjusting pH value, under the conditions of power 150-300W, frequency 2000Hz Carry out microwave extraction, wherein, each microwave exposure total time 60-80s, carry out compartment irradiation: irradiation 10s, be spaced 10s, Control temperature 20-35 DEG C, such irradiation 10 times, simultaneously at power 200-300W, carry out ultrasonic under the conditions of frequency 30-40KHz Ripple assisted extraction；Insulation 1-3h, then, carries out microwave extraction under the conditions of power 200-400W, frequency 2000Hz, wherein, Every time microwave exposure total time 90-105s, carry out compartment irradiation: irradiation 15s, be spaced 10s, control temperature 40-60 DEG C, as This irradiation 10 times, simultaneously at power 300-500W, carries out ultrasonic assistant extraction under the conditions of frequency 40-50KHz, finally natural It is cooled to room temperature, in electric field intensity 25-35kV/cm, burst length 400-600 μ s, carries out under the conditions of pulse frequency 200-300Hz High-pressure pulse electric extracts 15-20min；Extracting solution filters to obtain the first filtrate, adds the water rinsing of filtering residue 4 times, filters to obtain the second filter Liquid, uniformly mixes the first filtrate and the second filtrate 1:3-5 in mass ratio, and being evaporated to solid content is more than 20%, Obtain Fructus Hordei Germinatus extract；

Preferably, in described ultrasonic washing unit, cleanout fluid is the sodium bicarbonate solution of 0.3-0.5%.

Further, described Rhizoma Zingiberis Recens extract is to compound, Dioscorea zingiberensis and Rhizoma Zingiberis Recens science through high pressure after ultrasonic cleaning, freezing crushing Impulse electric field extracts and microwave extraction, after prepared through biological enzymolysis, in the present invention, its unique effect is can be the most anti- Only there is local, dispersibility recrystallization in the process of storage in high-load Mel probiotic bacteria honey composition, improves probiotic bacteria honey composition The storage stability of thing, extends the shelf life of probiotic bacteria honey composition, improves the commodity value of probiotic bacteria honey composition, and And also can work in coordination with the fragrance promoting natural honey, strengthen consumer's appetite；Certainly, it is also equipped with its material effective component simultaneously Functional characteristic；

Preferably, the preparation method of described Rhizoma Zingiberis Recens extract, comprise the steps: to be respectively put into Rhizoma Zingiberis Recens and Dioscorea zingiberensis equipped with The ultrasonic washing unit of 0.1-0.3% sodium bicarbonate solution cleans 5-10min in 200-400W, 30-40KHz, drains, by matter Measure more uniform mixing than 6-9:1-3, then in-20--24 DEG C of freezing 8-10min；It is crushed to particle diameter 0.2-0.4mm immediately；Put appearance In device and add the water of 4-6 times of weight, obtain mixed material, be 4.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 15-25kV/cm, carries out high voltage pulse electric field processing under the conditions of umber of pulse 30-50；Then heat to 30-40 DEG C, insulation, Carry out microwave irradiation and extraction, wherein, each microwave exposure total time 70s under the conditions of power 300-500W, carry out compartment irradiation: Irradiation 10s, is spaced 10s, such irradiation 10 times；It is continuously heating to 40-50 DEG C, insulation, first, add in extracting solution Metal ion so that metal ion content is: sodium ion 28-30mg/L, zinc ion 20-22mg/L, potassium ion 15-18mg/L, Calcium ion 10-12mg/L and magnesium ion 6-8mg/L, is subsequently adding the complex enzyme zymohydrolysis of mixed material gross weight 0.5-1.5% 40-60min, Bag filter, obtain filtrate；Filtering residue rinses 3 times with 2 times of weight 60-70 DEG C water, and rinsing liquid merges with filtrate, Uniformly mixing, being evaporated to solid content is more than 20%, obtains Rhizoma Zingiberis Recens extract；

Described compound enzyme quality group becomes: cellulase: glucanase: xylanase: seminase: laccase =5-7:3-5:3-5:2-4:1-3.

Further, described probiotic bacteria powder probiotics viable bacteria content is: 6 × 10¹²-8×10¹²cfu/g；

Described probiotic bacteria powder includes any one or more in following probiotic bacteria powder: such as: Lactobacillus plantarum (Lactobacillus plantarum), bifidobacterium bifidum (Bifidobacterium bifidum), bifidobacterium infantis (B.infantis), long bifidus bacillus (B.longum), short bifidus bacillus (B.breve), bifidobacterium adolescentis (B. Adolescentis), Bifidobacterium lactis (Bifidobacterium lactis), Lactobacillus bulgaricus (Lactobacillus. Bulgaricus), bacillus acidophilus (L.acidophilus), lactobacillus casei (Lactobacillus casei), rhamnose Lactobacillus (Lactobacillus rhamnosus), streptococcus thermophilus (Streptococcus thermophilus)；

Preferably, described probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 30-40 part, Bifidobacterium bifidum 25-35 part, Bifidobacterium lactis 20-30 part, streptococcus thermophilus 10-20 part, Lactobacillus bulgaricus 12-15 Part, bacillus acidophilus's 12-15 part, lactobacillus casei 8-10 part, lactobacillus rhamnosus 5-10 part；

It is highly preferred that described Lactobacillus plantarum powder is by side routinely with Lactobacillus plantarum CGMCC NO.11763 for the bacterium that sets out Prepared by method, cryoprotective agent therein with the animal or plant containing antifreeze protein as raw material, through high-pressure pulse electric extract, Ultrasonic assistant microwave extraction and compound enzyme enzymolysis and prepared, Lactobacillus plantarum powder can be effectively improved and live at freezing dry process Bacterium survival rate；

Preferably, described protectant preparation method, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen protein Being respectively washed, drain, 8-10:3-5:2-4 in mass ratio uniformly mixes, and the pH value adding mixed material quality 0.1-1 times is The lactic acid moistening 3-8h of 3.8-4.5, pulverizes after-18--22 DEG C of freezing 1-2h immediately, freezing thickness of feed layer 3-5cm, powder Mince particle diameter 0.5-3mm, is subsequently added into the water of ground product quality 10-20 times, is 3.5-5.5 with breast acid for adjusting pH value, in room temperature Under at electric field intensity 25-35kV/cm, burst length 300-500 μ s, carry out high-voltage pulse electric under the conditions of pulse frequency 200-300Hz Field processes 20-30min；Then under the conditions of power 150-300W, carry out microwave irradiation and extraction 15-20min in room temperature, exist simultaneously Power 200-300W, carries out ultrasonic assistant extraction under the conditions of frequency 30-40KHz；Add the compound enzyme of extracting solution quality 1-2%, In 45-55 DEG C of enzymolysis 30-50min；Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that 0.1-0.3mm i.e. obtains protection Agent；

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 3-5:2-4:1-3:1-3:1-2 in mass ratio Uniformly mixing；

Extract it is highly preferred that described microwave irradiation and extraction is batch (-type), i.e. microwave exposure 10s, be spaced 20s.

Further, any one during described Fruit powder is noni fruit powder, mango powder, pear powder, Fructus Hippophae powder, pueraria root powder.

Further, any one during described algae is Haematocoocus Pluvialls, salt alga, spirulina plalensis.

Further, any one during described bee pollen is Semen Sesami pollen, Pollen Brassicae campestris.

Further, described Chinese herbal medicine extract be with Bulbus Lilii, Flos Lonicerae, Herba Menthae, Fructus Momordicae, Colla Corii Asini, Flos Chrysanthemi, Radix Puerariae, In Fructus Jujubae, Fructus Cannabis, Semen Nelumbinis, Semen Ziziphi Spinosae, Rhizoma Polygonati, Fructus Lycii, Rhizoma Polygonati Odorati, the Radix Angelicae Dahuricae, Radix Ginseng, Folium Nelumbinis, Semen Cassiae, Japanese raisintree fruit One or more prepare for raw material；

Preferably, the preparation method of described Chinese herbal medicine extract, comprise the steps: to count by weight, weigh above-mentioned one Or several Chinese herbal medicine, put in the ultrasonic washing unit filling 0.1-0.3% sodium bicarbonate solution and clean in 200W, 30KHz 10-15min, drains, and above-mentioned Chinese medicine powder is broken to particle diameter is below 2mm, puts and uniformly mixes in container and add 3-6 times of weight Water, obtain mixed material, control temperature 70-90 DEG C and keep 2-4h, be then cooled to 40-50 DEG C, with breast acid for adjusting pH value be 3.5-4.5, carries out microwave extraction 10-15min under the conditions of power 150-300W, simultaneously in power 200-300W, frequency Ultrasonic assistant extraction is carried out under the conditions of 30-40KHz；The mixed enzyme being subsequently adding mixed material gross weight 0.5-1.5% carries out enzyme Solve, be 5.5-6.8 with breast acid for adjusting pH value, enzymolysis 2-4h, finally adds 0.5-3 times of w ethanol of mixed material and propanol Mixture, the mass ratio of ethanol and propanol mixing is 1:1-3, controls temperature and keeps 3-4h to 60-78 DEG C, filters, obtain first Filtrate；Add the water of 1-3 times of weight of filtering residue, control temperature 85-95 DEG C and keep 1-3h, be then cooled to 25-35 DEG C, filter, Obtain the second filtrate；First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, uniformly mixes, obtain Chinese herbal medicine extract；

Described mixed enzyme is glucanase, pentosanase, xylanase, acid protease, tannase 3-5 in mass ratio: 3-5:2-4:1-3:1-2 uniformly mixes.

Another object of the present invention is to provide the preparation method of above-mentioned probiotic bacteria honey composition, comprises the steps:

1) according to formula, first take fragrant-flowered garlic flower, clean, drain, be crushed to particle diameter 0.3-0.5mm, be added thereto to 20-50 part Mel and 2-5 part Fructus Hordei Germinatus extract, uniformly mix, and adds the deionized water of mixed material 2-4 times, with breast acid for adjusting pH value is 5-7, is warming up to 55-65 DEG C, be incubated 30-50min, be then cooled to 32-35 DEG C in electric field intensity 20-30kV/cm, during pulse Between 200-400 μ s, pulse frequency 200-400Hz carries out high-pressure pulse electric sterilization 5-10min, and adjustment pH value is 6.0-7.0, Add the 40-50% ferment at constant temperature 0.5-1h of probiotic bacteria powder quality, then with the ramp of 0.8-1.0 DEG C/min to 40-45 DEG C, Add the 50-60% ferment at constant temperature 2-4h of probiotic bacteria powder quality, finally with the ramp of 0.6-0.8 DEG C/min to 50-55 DEG C, Continuing fermentation 0.5-1h, fermentation liquid filters through 40 mesh, 80 mesh duplex strainers successively, and filtrate is concentrated into solid through loop ultrafiltration Thing content be more than 60% Mel probiotics fermention concentrated solution；

2) residue Mel is put in rustless steel container, is placed in equipped with in the pre-dissolving device of 50-60 DEG C of water, is pre-dissolved 30-50min, Cross 100-120 mesh sieve, proceed to blend tank, be incubated 50-60 DEG C, be sequentially added into residue Fructus Hordei Germinatus extract, Rhizoma Zingiberis Recens while stirring and carry Take thing, fully dissolve 10-15min；It is subsequently added into adjuvant and fully dissolves batch mixing at the beginning of 4-6min obtains；Then cross colloid mill to grind 4-6min, adjusts fineness of materials, crosses 160-200 mesh sieve, enters 80-83 DEG C of sterilizing 28-32min of sterilization tank, proceeds to basin cooling To 48-52 DEG C, last fill, seal, pack and i.e. obtain probiotic bacteria honey composition.

The probiotic bacteria honey composition crystallization time of occurrence prepared through said method is 36-48 month.

It is micro-that Lactobacillus plantarum of the present invention (Lactobacillus plantarum) XH is preserved in China on November 30th, 2015 CGMCC (is called for short) in biological inoculum preservation administration committee's common micro-organisms center, and preserving number is CGMCC NO.11763, preservation ground Location is: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.

Lactobacillus plantarum probiotic properties is as follows:

Lactobacillus plantarum CGMCC NO.11763 provided by the present invention is found under conditions of pH is 1.50 survive through experiment, Still in existing state after 1% cholate is cultivated 4 hours；Lactobacillus plantarum CGMCC NO.11763 degrading nitrite speed is fast, Capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at 4.8mg/kg Below；CGMCC NO.11763, after fermentation 60h hour, can reach 64.76% to degrading rate of cholesterol.CGMCC NO.11763 Adhering capacity measure from coagulation rate be 95.71%.

Lactobacillus plantarum CGMCC NO.11763 is to cholesterol degradation capability study and mensuration:

Take 1ml CGMCC NO.11763 mother solution and be inoculated in the MRS cholesterol fluid medium (cholesterol level of 10mL 0.1mg/ml, pH 6.2) in, it is standby, to access 1mL sterilized water that the constant temperature standing of 37 DEG C cultivates 20h, 40h, 60h respectively MRS cholesterol culture medium is comparison, takes bacteria liquid sample and the comparison each 1ml of liquid, 9000r/min, 4 DEG C of above cultivation different time Under be centrifuged 10min, obtain fermented supernatant fluid, o-phthalaldehyde method measures in supernatant cholesterol level (particularly as follows: take on each Clear liquid 0.1ml, in corresponding test tube, adds glacial acetic acid 0.3ml, and o-phthalaldehyde(OPA) 0.15ml of 1mg/ml is slowly added into dense sulfur Acid 1.0ml, mix homogeneously.Room temperature stands 10min, surveys light absorption value under 550nm).Each process 3 repetition, with equally Method makes cholesterol standard curve, calculates cholesterol level and degradation rate in supernatant, the results are shown in Table 1.Understand, CGMCCNO.11763 has good Degradation to cholesterol, and after fermentation 60h hour, degradation rate can reach 64.76%.

The table 1 degraded situation to cholesterol.

Degradation time (h)	0	20h	40h	60h
					Cholesterol level (mg/ml)	0.2273±0.0058	0.1356±0.0018	0.1011±0.0094	0.801±0.0231
Degrading rate of cholesterol %		40.34%	55.52%	64.76%

The bile tolerance test of Lactobacillus plantarum CGMCC NO.11763 bacterial strain:

Take CGMCCNO.11763 bacterium solution 1mL inoculation strain in containing different cholate (concentration gradients is 0.0%, 0.2%, 0.4%, 0.6%, 0.8%, 1%) 10mL MRS fluid medium (PH=6.4), be placed at 37 DEG C and cultivate 0 respectively, 2,4h, often Individual process 3 repetition.Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilution factor solution, take 0.1ml dilute Release liquid to be coated with in MRS, be inverted in 37 DEG C of biochemical cultivation cases and cultivate 48 hours (each dilution factor do 3 parallel) records Calculate the several number of bacterium on flat board.The results are shown in Table 2.The increment of bacterium is still after gallbladder salinity is 1% process 4h to understand this bacterium Reach 0.59 ± 0.92 × 10⁷(cfu/ml), there is good bile tolerance ability.

Table 2 bile tolerance ability detection [(± s) × 10⁷cfu/ml]

The acid resistance test of Lactobacillus plantarum CGMCC NO.11763 bacterial strain

Take HLX37 mother solution by 1ml inoculation strain in different pH value (pH gradient is 1.5,2.0,2.5,3.0,3.5,4.0) 10mLMRS fluid medium, be placed at 37 DEG C and cultivate 0 respectively, 2,4h, each process 3 repetition.Respectively take 1ml sample Product bacterium solution mixes in 9ml normal saline, prepares dilute solution, takes 0.1ml diluent and is coated with in MRS, in 37 DEG C Biochemical cultivation case is inverted the bacterium colony number cultivated on 48 hours (each dilution factor do 3 parallel) record flat board.Result is shown in Table 3.Illustrate that this bacterium has the strongest acid-fast ability.

Table 3 acid-fast ability detection [(± s) × 10⁷cfu/ml]

The Adhering capacity of Lactobacillus plantarum CGMCC NO.11763 measures

Cultivate CGMCC NO.11763 (MRS fluid medium), bacillus coli DH 5 alpha (LB fluid medium) 24h must send out Ferment liquid, is respectively placed in 3000r/min, centrifugal 10min at 4 DEG C, collects bacterium mud, delay with the sterile phosphate of pH=7.0 respectively Rush liquid (PBS) washing bacterium mud 2 times (in bacterium colony, i.e. add PBS, after concussion mix homogeneously, be placed in 3000r/min, 4 DEG C Under be centrifuged 10min, collect thalline).From coagulation rate (%): with aseptic PBS, bacterium mud CGMCC NO.11763 is formed in Light absorption value at wavelength 600nm is suspension bacteria liquid and the bacteria suspension of 0.4 ± 0.1 (A 0), measures extinction after standing 24h Value A 24, is (A 0 A 24)/A 0 from coagulation rate (%) formula.；His coagulation rate (%): by CGMCC NO.11763 It is adjusted to, with the outstanding bacterium solution of bacillus coli DH 5 alpha, the mixing that the light absorption value at wavelength 600nm is 0.6 ± 0.1 (A 0) hang Floating bacterium solution.Measuring light absorption value A 24 after standing 24H, his coagulation rate (%) formula is (A 0 A 24)/A 0.Survey Surely the results are shown in Table 5, it is known that CGMCC NO.11763 is 95.71% from coagulation rate, has the strongest Adhering capacity.

Table 4 Adhering capacity table

The bacterial strain physiological property of Lactobacillus plantarum CGMCC NO.11763

Described Lactobacillus plantarum (Lactobacillus plantarum) XH is preserved in Chinese micro-life on November 30th, 2015 CGMCC (is called for short) in thing culture presevation administration committee's common micro-organisms center, and preserving number is CGMCC NO.11763, preservation address For: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.

This bacterial strain feature is as follows: examine under a microscope, and this bacterial strain is rod-short, and Gram’s staining is positive, atrichia, no Produce spore；On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circular, and edge is more neat.

Physicochemical characteristics is: catalase (-), gelatin liquefaction (-), indole experiment (+), mobility (-), fermentation gas (-), nitrate reductase (-), fermentation gas (-), product hydrogen sulfide gas (-), pH4.0MRS culture medium grows (+). It is accredited as Lactobacillus plantarum (Lactobacillus plantarum), named Lactobacillus plantarum through Physiology and biochemistry (Lactobacillus plantarum)XH。

Bacterial strain can at 57 DEG C well-grown, glucose tolerance is 275g/L.

Lactobacillus plantarum of the present invention is by gathering people Li Jianshu, and isolated in Yoghourt from Xinjiang Uygur fellow-villager family, during collection Between on June 2nd, 2015.

Lactobacillus plantarum CGMCC NO.11763 of the present invention is found under conditions of pH is 1.50 survive, 1% through experiment Cholate is cultivated after 4 hours still in existing state；Lactobacillus plantarum CGMCC NO.11763 degrading nitrite speed is fast, decomposes Ability reaches 10.9mg/h/kg, this strain produce Pickles time, whole sweat nitrite concentration 4.8mg/kg with Under；CGMCCNO.11763, after fermentation 60h hour, can reach 64.76% to degrading rate of cholesterol.CGMCC NO.11763 sticks Attached ability measure from coagulation rate be 95.71%, bacterial strain can at 57 DEG C well-grown, glucose tolerance is 275g/L.

Beneficial effect:

The present invention is with Mel as primary raw material, first with part Mel, Fructus Hordei Germinatus extract and fragrant-flowered garlic flower, probiotic bacteria powder as raw material, Through techniques such as low temperature enzymolysis, high-pressure pulse electric sterilization, the inoculation of temperature-variable fermentation, by several times probiotic bacteria, ultrafiltration concentrations, prepare acid Comfortable preferably, probiotic bacteria content and the high Mel probiotics fermention concentrated solution of functional metabolic thing content thereof, its probiotic bacteria content up to 8.12×10¹²-9.58×10¹²CFU/ml, probiotic extremely strong, then with residue Mel, Fructus Hordei Germinatus extract and Rhizoma Zingiberis Recens extract and merit A kind of honey content height prepared by energy property adjuvant, sweet mouthfeel preservation suitable, long-term (36-48 month) does not crystallizes, keep healthy effect Fruit significantly probiotic bacteria honey composition.Concrete technique effect is shown in embodiment seven to nine；Concrete know-why is as follows:

1. the probiotics science of the present invention compounds various lactobacillus agent, and kind is many, and function is complete, probiotic by force, particularly plant Lactobacillus CGMCC NO.11763 is found under conditions of pH is 1.50 survive through experiment, cultivates 4 hours at 1% cholate After still in existing state；Lactobacillus plantarum CGMCC NO.11763 degrading nitrite speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, this strain is when producing Pickles, and whole sweat nitrite concentration is at below 4.8mg/kg；CGMCC NO.11763, after fermentation 60h hour, can reach 64.76% to degrading rate of cholesterol.CGMCC NO.11763 Adhering capacity is surveyed Fixed from coagulation rate be 95.71%.

It should be noted that containing substantial amounts of functional probiotic bacteria metabolite and dead thalline in probiotic bacteria honey composition of the present invention, though For dead thalline, but dead thalline, cell debris are as metabolite and viable bacteria, can suppress pathogen to the adhesion of intestinal wall and invasion and attack, Organic regulation human body overall immunity, enhancing macrophages phagocytic capacity, suppression tumor growth, raising natural killer cell (NK Cell) activity, improve mucomembranous surface pathogenic bacteria and lysozyme balance etc., its effect is in direct ratio with dead thalline quantity, inactivation Mel probiotics fermention concentrated solution is likewise supplied with the stronger probiotic of its probiotic bacteria.

2. the cryoprotective agent that the present invention uses when preparing Lactobacillus plantarum powder is by the winter rye higher containing antifreeze protein, sand Ilicis Purpureae and sharkskin collagen protein science compound, through high-pressure pulse electric extraction, ultrasonic assistant microwave extraction and biological enzymolysis Preparing, omnidistance extract at low temperature, antifreeze protein extraction ratio is high, and loss is few, the protective agent antifreeze peptide content obtained is high, kind is complete, Functional strong, cryoprotective effects is good, improves the viable bacteria content in Lactobacillus plantarum powder.

3. the Fructus Hordei Germinatus extract that prepared by the present invention does not contain only abundant plant rennet, amylase, hemicellulase, esterase, The various plants enzymes such as oxidoreductase and containing vegetable polysaccharides and monosaccharide, plant amylum, vegetable protein, soluble fiber etc. seek Support material, can be not only that probiotic bacteria honey composition provides comprehensive, natural phytoenzyme, can be also that probiotic bacteria provides comprehensive, rich Rich nutrient substance；It is compounded with Rhizoma Zingiberis Recens extract science simultaneously, the storage stability strengthening Mel can be worked in coordination with, and then can prevent Only Mel recrystallization, through extract at low temperature such as ultrasonic cleaning, microwave-assisted supersound extraction and high-pressure pulse electric extraction, concentrating under reduced pressure After, its effect is more significantly, and is effectively increased raw material availability, phytoenzyme activity and productivity；Fructus Hordei Germinatus is effectively ensured carry Take the foodsafety of thing.

4. the Rhizoma Zingiberis Recens extract that prepared by the present invention is to compound, Dioscorea zingiberensis and Rhizoma Zingiberis Recens science through high pressure after ultrasonic cleaning, freezing crushing Impulse electric field extracts and microwave extraction, after prepared through biological enzymolysis, in the present invention, its unique effect is effectively to carry High high-load Mel probiotic bacteria honey composition, in the stability of the process of storage, improves the presentation quality of product, prevents probiotic bacteria honeybee There is local, dispersibility recrystallization in honey compositions under the environmental conditions such as temperature, moisture, microorganism pollution, extends probiotic bacteria honeybee The shelf life of honey compositions, improves the commodity value of probiotic bacteria honey composition, and also can work in coordination with the perfume (or spice) promoting natural honey Gas, strengthens consumer's appetite, compounds with Fructus Hordei Germinatus extract science, and synergism is higher；Certainly, it is also equipped with Dioscorea zingiberensis and life simultaneously The functional characteristic of Rhizoma Zingiberis Recens effective ingredient.

5. fragrant-flowered garlic flower science is compounded in probiotic bacteria honey composition by the present invention, and breaching traditional fragrant-flowered garlic flower can not be with food with Mel Theory, compounds a small amount of fragrant-flowered garlic flower with Mel, Fructus Hordei Germinatus extract science, and through probiotics fermention, not only will not produce stomachache, abdomen The symptom such as rush down, but also Mel digestion can be promoted, absorb, prevent flatulence, be particularly suitable for gastric acid, stomach because of edible Mel Swollen, diarrhoea consumer, has expanded the consumer group of probiotic bacteria honey composition.

Science the most of the present invention has compounded the Fruit powder such as noni fruit powder, mango powder, pear powder, Fructus Hippophae powder, pueraria root powder；The raw red ball of rain The algae such as algae, salt alga, spirulina plalensis；Aloe vera gel；The bee pollen such as Semen Sesami pollen, Pollen Brassicae campestris；Oligomeric xylose； Oligochitosan；Marine fishbone collagen oligopeptide powder；Bulbus Lilii, Flos Lonicerae, Herba Menthae, Fructus Momordicae, Colla Corii Asini, Fructus Jujubae, Fructus Cannabis, lotus The Chinese herbal medicine extracts such as son, Semen Ziziphi Spinosae, Rhizoma Polygonati, Fructus Lycii, Rhizoma Polygonati Odorati, the Radix Angelicae Dahuricae, Radix Ginseng, Folium Nelumbinis, Semen Cassiae, Japanese raisintree fruit；Pupa Cordyceps；Using one or more in above-mentioned raw materials as adjuvant, more enhance functional characteristic and the guarantor of probiotic bacteria honey composition Strong effect, can prepare several functions Sexual health promotion series probiotic bacteria honey composition.

It should be noted that probiotic bacteria honey composition of the present invention has the technical effect that each component technical characteristic is mutually collaborative, phase interaction Result, the superposition of not simple technical characteristic (component function), the combination of each component technical characteristic and collaborative produce Raw effect, considerably beyond each monotechnics feature functionality and the superposition of effect, has the most advanced and practicality.

Detailed description of the invention

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is this Method well known to skilled person.It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, The spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, real without departing substantially from the present invention On the premise of matter and scope, the various changes or the change that carry out the material component in these embodiments and consumption fall within this Bright protection domain.

Embodiment one:

Prepared by raw material

1, the preparation of Fructus Hordei Germinatus extract

Its preparation method is:

Fructus Hordei Germinatus and Fructus Tritici aestivi 9:2 in mass ratio are uniformly mixed, is crushed to granularity 0.8mm, obtains pulverizing Fructus Hordei Germinatus；Then by Fructus Chaenomelis, Fructus Ananadis comosi, Fructus Fici in the ultrasonic washing unit equipped with 0.4% sodium bicarbonate solution in power 200W, frequency 30KHz condition Lower ultrasonic cleaning 8min, drains, and is crushed to granularity 0.8mm, and 8:2:1.5 uniformly mixes in mass ratio under room temperature, adds mixed The pulverizing Fructus Hordei Germinatus of compound quality 4 times obtains raw mixture, adds the water of raw mixture quality 2 times, uses Fructus Citri Limoniae acid for adjusting pH value It is 3.5, under the conditions of power 225W, frequency 2000Hz, carries out microwave extraction, wherein, each microwave exposure total time 70s, Carry out compartment irradiation: irradiation 10s, interval 10s, control temperature 28 DEG C, such irradiation 10 times, simultaneously at power 250W, frequently Ultrasonic assistant extraction is carried out under the conditions of rate 35KHz；Insulation 2h, then, is carried out under the conditions of power 300W, frequency 2000Hz Microwave extraction, wherein, each microwave exposure total time 95s, carries out compartment irradiation: irradiation 15s, interval 10s, control is warm Spend 50 DEG C, such irradiation 10 times, simultaneously at power 400W, carry out ultrasonic assistant extraction under the conditions of frequency 45KHz, finally It is naturally cooling to room temperature, in electric field intensity 30kV/cm, burst length 500 μ s, under the conditions of pulse frequency 250Hz, carries out high-tension pulse Rush electric field and extract 18min；Extracting solution filters to obtain the first filtrate, adds the water rinsing of filtering residue 4 times, filters to obtain the second filtrate, by the One filtrate and the second filtrate 1:4 in mass ratio uniformly mix, and being evaporated to solid content is 30%, obtains Fructus Hordei Germinatus extract.

2, the preparation of Rhizoma Zingiberis Recens extract

Its preparation method, comprises the steps:

Rhizoma Zingiberis Recens and Dioscorea zingiberensis are respectively put in the ultrasonic washing unit equipped with 0.2% sodium bicarbonate solution and clean in 300W, 35KHz 8min, drains, and 7.5:2 in mass ratio uniformly mixes, then in-22 DEG C of freezing 9min；It is crushed to particle diameter 0.3mm immediately；Put In container and add the water of 5 times of weight, obtain mixed material, be 4.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 20kV/cm, carries out high voltage pulse electric field processing under the conditions of umber of pulse 40；Then heating to 35 DEG C, insulation, at power 400W Under the conditions of carry out microwave irradiation and extraction, wherein, each microwave exposure total time 70s, carry out compartment irradiation: irradiation 10s, Every 10s, such irradiation 10 times；It is continuously heating to 45 DEG C, insulation, first, in extracting solution, add metal ion so that gold Belonging to ion concentration is: sodium ion 29mg/L, zinc ion 21mg/L, potassium ion 16.5mg/L, calcium ion 11mg/L and magnesium ion 7mg/L, is subsequently adding the complex enzyme zymohydrolysis 50min of mixed material gross weight 1.0%, Bag filter, obtains filtrate；Filtering residue is with 2 times 65 DEG C of water of weight rinse 3 times, and rinsing liquid merges with filtrate, uniformly mixes, and being evaporated to solid content is 30%, to obtain final product Rhizoma Zingiberis Recens extract；

Above-mentioned compound enzyme quality group becomes: cellulase: glucanase: xylanase: seminase: laccase=6:4:4:3:2.

3, the preparation of Lactobacillus plantarum powder

Lactobacillus plantarum powder with Lactobacillus plantarum CGMCC NO.11763 for the bacterium that sets out by preparing according to a conventional method, therein Cryoprotective agent, with the animal or plant containing antifreeze protein as raw material, carries through high-pressure pulse electric extraction, ultrasonic assistant microwave Take with compound enzyme enzymolysis and prepared, Lactobacillus plantarum powder can be effectively improved at freezing dry process Viable detection；

Above-mentioned protectant preparation method, comprises the steps:

Winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen protein are respectively washed, are drained, and 9:4:3 in mass ratio uniformly mixes, and adds The pH value that mixed material quality is 0.5 times is the lactic acid moistening 6h of 4.2, pulverizes immediately after-20 DEG C of freezing 1.5h, freezing Thickness of feed layer 4cm, ground product particle diameter 2mm, it is subsequently added into the water of ground product quality 15 times, is 4.5 with breast acid for adjusting pH value, At room temperature in electric field intensity 30kV/cm, burst length 400 μ s, carry out at high-pressure pulse electric under the conditions of pulse frequency 250Hz Reason 25min；Then under the conditions of power 225W, microwave irradiation and extraction 18min is carried out in room temperature, simultaneously in power 250W, frequency Ultrasonic assistant extraction is carried out under the conditions of 35KHz；Add the compound enzyme of extracting solution quality 1.5%, in 50 DEG C of enzymolysis 40min；Enzyme Solve liquid filtration, filtrate concentrates, low-temperature grinding to particle diameter is that 0.2mm i.e. obtains protective agent；

Above-mentioned compound enzyme is that cellulase, protease, amylase, pectase, tannase 4:3:2:2:1.5 in mass ratio is uniform Mixing；

Above-mentioned microwave irradiation and extraction is that batch (-type) extracts, i.e. microwave exposure 10s is spaced 20s.

Used by following example two to six, Fructus Hordei Germinatus extract, Rhizoma Zingiberis Recens extract, Lactobacillus plantarum powder are prepared by embodiment one.

Embodiment two:

A kind of Haematocoocus Pluvialls probiotic bacteria honey composition, is mainly prepared by the raw material of following parts by weight:

Mel 170 parts, Fructus Hordei Germinatus extract 7 parts, Rhizoma Zingiberis Recens extract 4 parts, 2 parts of probiotic bacteria powder, fragrant-flowered garlic spends 2 parts, and rain is raw red 0.8 part of ball algae；

Wherein Mel contains the Flos Robiniae Pseudoacaciae honey of 90%；

Above-mentioned probiotic bacteria powder probiotics viable bacteria content is: 7 × 10¹²cfu/g；

Probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 35 parts, bifidobacterium bifidum 30 Part, Bifidobacterium lactis 25 parts, streptococcus thermophilus 15 parts, Lactobacillus bulgaricus 13 parts, bacillus acidophilus 13 parts, cheese Lactobacillus 9 parts, lactobacillus rhamnosus 8 parts；

The preparation method of Haematocoocus Pluvialls probiotic bacteria honey composition, comprises the steps:

1) according to formula, first take fragrant-flowered garlic flower, clean, drain, be crushed to particle diameter 0.4mm, be added thereto to 35 parts of Mel and 2.5 Part Fructus Hordei Germinatus extract, uniformly mixes, and adds the deionized water of mixed material 3 times, is 6 with breast acid for adjusting pH value, is warming up to 60 DEG C, Insulation 40min, be then cooled to 33.5 DEG C in electric field intensity 25kV/cm, burst length 300 μ s, pulse frequency 300Hz is carried out High-pressure pulse electric sterilization 8min, adjusting pH value is 6.5, adds 45% ferment at constant temperature 0.8h of probiotic bacteria powder quality, then With the ramp of 0.9 DEG C/min to 43 DEG C, add 55% ferment at constant temperature 3h of probiotic bacteria powder quality, finally with 0.7 DEG C/min Ramp to 52.5 DEG C, continue fermentation 0.8h, fermentation liquid successively through 40 mesh, 80 mesh duplex strainers filter, filtrate warp Loop ultrafiltration be concentrated into solid content be 70% Mel probiotics fermention concentrated solution；

2) residue Mel is put in rustless steel container, be placed in equipped with in the pre-dissolving device of 55 DEG C of water, be pre-dissolved 40min, cross 110 Mesh sieve, proceeds to blend tank, is incubated 55 DEG C, is sequentially added into residue Fructus Hordei Germinatus extract, Rhizoma Zingiberis Recens extract while stirring, fully dissolves 13min；It is subsequently added into Haematocoocus Pluvialls and fully dissolves batch mixing at the beginning of 5min obtains；Then cross colloid mill and grind 5min, adjust material thin Degree, crosses 180 mesh sieves, enters 82 DEG C of sterilizing 30min of sterilization tank, proceed to basin and be cooled to 50 DEG C, last fill, seals, packs Obtain Haematocoocus Pluvialls probiotic bacteria honey composition.

The probiotic bacteria honey composition crystallization time of occurrence prepared through said method is 48 months.

Embodiment three:

A kind of Flos Chrysanthemi Radix Puerariae probiotic bacteria honey composition, is mainly prepared by the raw material of following parts by weight:

Mel 165 parts, Fructus Hordei Germinatus extract 6 parts, Rhizoma Zingiberis Recens extract 3 parts, 1.5 parts of probiotic bacteria powder, fragrant-flowered garlic spends 1.5 parts, in Herb extracts 0.3 part, mango powder 0.9 part；

Wherein Mel contains the Flos Robiniae Pseudoacaciae honey of 80%；

Above-mentioned probiotic bacteria powder probiotics viable bacteria content is: 6 × 10¹²cfu/g；

Probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 30 parts, bifidobacterium bifidum 25 Part, Bifidobacterium lactis 20 parts, streptococcus thermophilus 10 parts, Lactobacillus bulgaricus 12 parts, bacillus acidophilus 12 parts, cheese Lactobacillus 8 parts, lactobacillus rhamnosus 5 parts；

Said herbal medicine extract is prepared with Flos Chrysanthemi, Radix Puerariae, red date as raw materials；

Its preparation method comprises the steps:

Count by weight, weigh above-mentioned Flos Chrysanthemi, Radix Puerariae, Fructus Jujubae, put the ultrasonic washing unit filling 0.2% sodium bicarbonate solution In clean 13min in 200W, 30KHz, drain, above-mentioned Flos Chrysanthemi, Radix Puerariae, red date powder are broken to particle diameter is 1mm, puts in container Uniformly mix and add the water of 5 times of weight, obtain mixed material, control temperature 80 DEG C and keep 3h, be then cooled to 45 DEG C, use Breast acid for adjusting pH value is 4.0, carries out microwave extraction 13min under the conditions of power 225W, simultaneously in power 250W, frequency 35KHz Under the conditions of carry out ultrasonic assistant extraction；The mixed enzyme being subsequently adding mixed material gross weight 1.0% carries out enzymolysis, regulates with lactic acid PH value is 6.2, enzymolysis 3h, finally adds 2 times of w ethanol of mixed material and the mixture of propanol, ethanol and propanol mixing Mass ratio is 1:2, controls temperature and keeps 3.5h to 69 DEG C, filters, obtain the first filtrate；Add the water of 2 times of weight of filtering residue, control Temperature processed 90 DEG C keeps 2h, is then cooled to 30 DEG C, filters, obtains the second filtrate；By the first filtrate and the second filtrate according to matter Measure and merge than 3:2, uniformly mix, obtain Chinese herbal medicine extract；

Above-mentioned mixed enzyme is glucanase, pentosanase, xylanase, acid protease, tannase 4:4:3:2:1.5 in mass ratio Uniformly mixing.

The preparation method of Flos Chrysanthemi Radix Puerariae probiotic bacteria honey composition, comprises the steps:

1) according to formula, first take fragrant-flowered garlic flower, clean, drain, be crushed to particle diameter 0.3mm, be added thereto to 20 parts of Mel and 2 Part Fructus Hordei Germinatus extract, uniformly mixes, and adds the deionized water of mixed material 2 times, is 5 with breast acid for adjusting pH value, is warming up to 55 DEG C, Insulation 30min, be then cooled to 32 DEG C in electric field intensity 20kV/cm, burst length 200 μ s, pulse frequency 200Hz carries out height Pressure impulse electric field sterilization 5min, adjust pH value be 6.0, add probiotic bacteria powder quality 40% ferment at constant temperature 0.5h, then with The ramp of 0.8 DEG C/min, to 40 DEG C, adds 50% ferment at constant temperature 2h of probiotic bacteria powder quality, finally with 0.6 DEG C/min Ramp to 50 DEG C, continue fermentation 0.5h, fermentation liquid successively through 40 mesh, 80 mesh duplex strainers filter, filtrate is through following Ring be concentrated by ultrafiltration to solid content be 65% Mel probiotics fermention concentrated solution；

2) residue Mel is put in rustless steel container, be placed in equipped with in the pre-dissolving device of 50 DEG C of water, be pre-dissolved 30min, cross 100 Mesh sieve, proceeds to blend tank, is incubated 50 DEG C, is sequentially added into residue Fructus Hordei Germinatus extract, Rhizoma Zingiberis Recens extract while stirring, fully dissolves 10min；It is subsequently added into Chinese herbal medicine extract, mango powder, fully dissolves 4min and obtain just batch mixing；Then cross colloid mill and grind 4min, Adjust fineness of materials, cross 160 mesh sieves, enter 80 DEG C of sterilizing 28min of sterilization tank, proceed to basin and be cooled to 48 DEG C, last fill, Sealing, pack and i.e. obtain Flos Chrysanthemi Radix Puerariae probiotic bacteria honey composition.

The probiotic bacteria honey composition crystallization time of occurrence prepared through said method is 45 months.

Embodiment four:

A kind of oligomeric xylose probiotic bacteria honey composition, is mainly prepared by the raw material of following parts by weight:

Mel 175 parts, Fructus Hordei Germinatus extract 8 parts, Rhizoma Zingiberis Recens extract 5 parts, 2.5 parts of probiotic bacteria powder, fragrant-flowered garlic spends 2.5 parts, low Xylan 0.5 part；

Wherein Mel contains the Flos Robiniae Pseudoacaciae honey of 99%；

Above-mentioned probiotic bacteria powder probiotics viable bacteria content is: 8 × 10¹²cfu/g；

Probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 40 parts, bifidobacterium bifidum 35 Part, Bifidobacterium lactis 30 parts, streptococcus thermophilus 20 parts, Lactobacillus bulgaricus 15 parts, bacillus acidophilus 15 parts, cheese Lactobacillus 10 parts, lactobacillus rhamnosus 10 parts；

The preparation method of oligomeric xylose probiotic bacteria honey composition, comprises the steps:

1) according to formula, first take fragrant-flowered garlic flower, clean, drain, be crushed to particle diameter 0.5mm, be added thereto to 50 parts of Mel and 5 Part Fructus Hordei Germinatus extract, uniformly mixes, and adds the deionized water of mixed material 4 times, is 7 with breast acid for adjusting pH value, is warming up to 65 DEG C, Insulation 50min, be then cooled to 35 DEG C in electric field intensity 30kV/cm, burst length 400 μ s, pulse frequency 400Hz carries out height Pressure impulse electric field sterilization 10min, adjust pH value be 7.0, add probiotic bacteria powder quality 50% ferment at constant temperature 1h, then with The ramp of 1.0 DEG C/min, to 45 DEG C, adds 60% ferment at constant temperature 4h of probiotic bacteria powder quality, finally with 0.8 DEG C/min Ramp to 55 DEG C, continue fermentation 1h, fermentation liquid successively through 40 mesh, 80 mesh duplex strainers filter, filtrate through circulation Be concentrated by ultrafiltration to solid content be 75% Mel probiotics fermention concentrated solution；

2) residue Mel is put in rustless steel container, be placed in equipped with in the pre-dissolving device of 60 DEG C of water, be pre-dissolved 50min, cross 120 Mesh sieve, proceeds to blend tank, is incubated 60 DEG C, is sequentially added into residue Fructus Hordei Germinatus extract, Rhizoma Zingiberis Recens extract while stirring, fully dissolves 15min；It is subsequently added into oligomeric xylose and fully dissolves batch mixing at the beginning of 6min obtains；Then cross colloid mill and grind 6min, adjust fineness of materials, Cross 200 mesh sieves, enter 83 DEG C of sterilizing 32min of sterilization tank, proceed to basin and be cooled to 52 DEG C, last fill, seal, pack and get final product Oligomeric xylose probiotic bacteria honey composition.

The probiotic bacteria honey composition crystallization time of occurrence prepared through said method is 40 months.

Embodiment five:

A kind of Cordyceps militaris (L.) Link. probiotic bacteria honey composition, is mainly prepared by the raw material of following parts by weight:

Mel 155 parts, Fructus Hordei Germinatus extract 4 parts, Rhizoma Zingiberis Recens extract 2 parts, 1 part of probiotic bacteria powder, fragrant-flowered garlic spends 1 part, Cordyceps militaris (L.) Link. 0.3 part；

Wherein Mel contains the Flos Robiniae Pseudoacaciae honey of 85%；

Above-mentioned probiotic bacteria powder probiotics viable bacteria content is: 6.5 × 10¹²cfu/g；

Probiotic bacteria powder is Lactobacillus plantarum powder；

The preparation method of Cordyceps militaris (L.) Link. probiotic bacteria honey composition, comprises the steps:

1) according to formula, first take fragrant-flowered garlic flower, clean, drain, be crushed to particle diameter 0.35mm, be added thereto to 30 parts of Mel and 3 Part Fructus Hordei Germinatus extract, uniformly mixes, and adds the deionized water of mixed material 2.5 times, is 5.5 with breast acid for adjusting pH value, heats up To 58 DEG C, it is incubated 35min, is then cooled to 33 DEG C in electric field intensity 23kV/cm, burst length 250 μ s, pulse frequency 250Hz Carrying out high-pressure pulse electric sterilization 7min, adjusting pH value is 6.3, adds 43% ferment at constant temperature 0.7h of probiotic bacteria powder quality, Then with the ramp of 0.85 DEG C/min to 42 DEG C, add 53% ferment at constant temperature 2.5h of probiotic bacteria powder quality, finally with The ramp of 0.65 DEG C/min, to 52 DEG C, continues fermentation 0.6h, and fermentation liquid filters through 40 mesh, 80 mesh duplex strainers successively, Filtrate through loop ultrafiltration be concentrated into solid content be 80% Mel probiotics fermention concentrated solution；

2) residue Mel is put in rustless steel container, be placed in equipped with in the pre-dissolving device of 53 DEG C of water, be pre-dissolved 35min, cross 105 Mesh sieve, proceeds to blend tank, is incubated 53 DEG C, is sequentially added into residue Fructus Hordei Germinatus extract, Rhizoma Zingiberis Recens extract while stirring, fully dissolves 12min；It is subsequently added into Cordyceps militaris (L.) Link. and fully dissolves batch mixing at the beginning of 4.5min obtains；Then cross colloid mill and grind 4.5min, adjust material thin Degree, crosses 170 mesh sieves, enters 81 DEG C of sterilizing 29min of sterilization tank, proceed to basin and be cooled to 49 DEG C, last fill, seals, packs Obtain Cordyceps militaris (L.) Link. probiotic bacteria honey composition.

The probiotic bacteria honey composition crystallization time of occurrence prepared through said method is 36 months.

Embodiment six:

A kind of oligochitosan probiotic bacteria honey composition, is mainly prepared by the raw material of following parts by weight:

Mel 185 parts, Fructus Hordei Germinatus extract 10 parts, Rhizoma Zingiberis Recens extract 6 parts, 3 parts of probiotic bacteria powder, fragrant-flowered garlic spends 3 parts, oligochitosan 0.4 part；

Wherein Mel contains the Flos Robiniae Pseudoacaciae honey of 95%；

Above-mentioned probiotic bacteria powder probiotics viable bacteria content is: 7.5 × 10¹²cfu/g；

Probiotic bacteria powder is lactobacillus casei powder；

The preparation method of oligochitosan probiotic bacteria honey composition, comprises the steps:

1) according to formula, first take fragrant-flowered garlic flower, clean, drain, be crushed to particle diameter 0.45mm, be added thereto to 40 parts of Mel and 4 Part Fructus Hordei Germinatus extract, uniformly mixes, and adds the deionized water of mixed material 3.5 times, is 6.5 with breast acid for adjusting pH value, heats up To 63 DEG C, it is incubated 45min, is then cooled to 34 DEG C in electric field intensity 28kV/cm, burst length 350 μ s, pulse frequency 350Hz Carrying out high-pressure pulse electric sterilization 9min, adjusting pH value is 6.8, adds 48% ferment at constant temperature 0.9h of probiotic bacteria powder quality, Then with the ramp of 0.95 DEG C/min to 44 DEG C, add 58% ferment at constant temperature 3.5h of probiotic bacteria powder quality, finally with The ramp of 0.75 DEG C/min, to 54 DEG C, continues fermentation 0.9h, and fermentation liquid filters through 40 mesh, 80 mesh duplex strainers successively, Filtrate through loop ultrafiltration be concentrated into solid content be 85% Mel probiotics fermention concentrated solution；

2) residue Mel is put in rustless steel container, be placed in equipped with in the pre-dissolving device of 58 DEG C of water, be pre-dissolved 45min, cross 115 Mesh sieve, proceeds to blend tank, is incubated 58 DEG C, is sequentially added into residue Fructus Hordei Germinatus extract, Rhizoma Zingiberis Recens extract while stirring, fully dissolves 14min；It is subsequently added into oligochitosan and fully dissolves batch mixing at the beginning of 5.5min obtains；Then cross colloid mill and grind 5.5min, adjust material thin Degree, crosses 190 mesh sieves, enters 82 DEG C of sterilizing 31min of sterilization tank, proceed to basin and be cooled to 51 DEG C, last fill, seals, packs Obtain oligochitosan probiotic bacteria honey composition.

The probiotic bacteria honey composition crystallization time of occurrence prepared through said method is 38 months.

Experimental example seven eats the change of T-CHOL after Haematocoocus Pluvialls probiotic bacteria honey composition

Selecting the adult 48 of T-CHOL 180mg/dl-250mg/dl, men and women half and half, is randomly divided into three groups；First group of every day 150 milliliters of mineral waters are drunk in dinner；Second group of common commercially available Haematocoocus Pluvialls processed with honey cream 150g of dinner every day Instant Drinks, the 3rd group every The Haematocoocus Pluvialls probiotic bacteria honey composition 150g of it dinner Instant Drinks embodiment of the present invention 2, eats same food every day period, Food includes meat, egg, vegetable and fruit.Gather experimenter's in the previous day and the 20th, 40,60 days that experiment starts respectively Blood, measures the total cholesterol level in blood, result such as table 1:

Table 1: total cholesterol level testing result in blood

Time	0 day	20 days	40 days	60 days
					First group (mg/dl)	202.3	203.8	206.2	209.3
Second group (mg/dl)	207.6	206.5	203.2	202.1
					3rd group (mg/dl)	210.5	201.9	189.7	178.6

As seen from the above table after the Haematocoocus Pluvialls probiotic bacteria honey composition of the Instant Drinks embodiment of the present invention 2, total in adult's blood The content generation significant change of cholesterol.Compared with common commercially available Haematocoocus Pluvialls processed with honey cream, Haematocoocus Pluvialls probiotic bacteria of the present invention The content of the T-CHOL in adult's blood is significantly reduced by honey composition, and the total gallbladder in mineral water composition year human blood The content of sterin significantly increases, although commercially available Haematocoocus Pluvialls processed with honey cream decreases, but compared with product of the present invention, and effect It is not notable, it follows that the present invention uses to have reduces Haematocoocus Pluvialls probiotic bacteria honeybee prepared by the specific bacterial strain of cholesterol characteristic Honey compositions has the health-care effect well reducing cholesterol.

It should be understood that the probiotic bacteria honey composition prepared by embodiment of the present invention 3-6 has above-mentioned technique effect equally, Between each embodiment, diversity is the most notable.

The sensory evaluation test of embodiment eight Haematocoocus Pluvialls of the present invention probiotic bacteria honey composition

Invite Haematocoocus Pluvialls probiotic bacteria honey composition and commercially available two kinds of similar phases that the embodiment of the present invention 2 prepared by 24 personnel Judging with the Haematocoocus Pluvialls processed with honey cream of date of manufacture, sense organ is given a mark, wherein specialty and each 12 of layman, specialty Personnel are young, middle aged, each 4 of old age, and men and women half and half, layman teenager, youth, middle age, each 3 of old age, male Female half and half；Marking includes outward appearance (20 points), quality (25 points), local flavor (30 points), four aspects of mouthfeel (25 points), beats Divide personnel independently to carry out, be independent of each other, judge result with guarantee accurate.Being added up judging result, equal score value takes approximation Value, retains integer, is specifically shown in Table 2:

Table 2: sensory evaluation statistical result

Note: show significant difference (P ＜ 0.05) with a line internal standard difference lowercase alphabet, the different capitalization of mark represents that difference is extremely notable (P ＜ 0.01), Indicate same letter and represent that difference is not notable (P ＞ 0.05).

Result above shows, Haematocoocus Pluvialls probiotic bacteria honey composition prepared by the present invention is appointed from outward appearance, quality, local flavor and mouthfeel On the one hand what will be substantially better than commercially available Haematocoocus Pluvialls processed with honey cream, and particularly outward appearance, local flavor and mouthfeel is fabulous, also is adapted for simultaneously Different age group, the consumer of different hierarchy of consumption eat.

It should be understood that probiotic bacteria honey composition prepared by embodiment of the present invention 3-6 has above-mentioned experiment effect equally, respectively Between embodiment and little with above-mentioned experiment effect diversity.

The impact on immunity of organisms of the embodiment nine probiotic bacteria of the present invention honey composition

1 experiment purpose

By exercise tolerance test (mouse forced swimming), verify probiotic bacteria honey composition of the present invention raising immunity, resist tired Work is used.

2 experiment materials and reagent

2.1 for reagent thing:

Commercially available probiotic bacteria honey composition (G1)；Commercially available probiotic bacteria honey composition (G2)；Prepared by embodiment of the present invention 2-6 Probiotic bacteria honey composition (G3-G7).

2.2 reagent:

Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing；Concentrated sulphuric acid (AR), the limited public affairs of Nanjing chemical reagent Department；Normal saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..

3. laboratory animal

ICR mice, ♂, cleaning grade, body weight 18-22g, Ningxia Medical University's comparative medicine center provide, little during experiment The free diet of Mus.

4. key instrument

Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof is public Department；Water-bath: DK-S26 type, upper Nereid grand experimental facilities company limited；Electronic scale: BS224S type, Sartorius Company；Stopwatch, thermometer

5. experiment packet

5.1 dosage packets and given the test agent give the time and at random mice are divided into 8 groups, and often group 10, the 1st group to the 7th component Not Gei the medicine of G1～G7, the 8th group is blank group, gives isopyknic distilled water, the often every average daily gavage of group 1 time, Gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.

5.2 sample preparations the 1st group are to the 7th group: weigh 2.25g drug sample, be assigned to 150ml with distilled water；Blank Group: distilled water 150ml.

6. experimental technique

6.1 swimmings with a load attached to the body experiment lasts are administered after 30min, put mice in swimming trunk, and the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of rat-tail root load 5% body weight, record mice swimming starts to the dead time, when swimming as mice Between.

After 6.2 mice serum carbamide measure last administration 30min, not swimming with a load attached to the body 90min in the water that temperature is 30 DEG C, Pluck after rest 60min eyeball blood sampling 0.5mL (being not added with anticoagulant), put 4 DEG C of refrigerator 3h, after hemopexis 2000r/min from Heart 15min, takes serum and send clinical laboratory of Affiliated Hospital of Ningxia Medical University to detect.

After the mensuration last of 6.3 hepatic glycogen is administered 30min, not swimming with a load attached to the body 90min in the water that temperature is 25 ± 1 DEG C, cervical vertebra Mice is put to death in dislocation, cleans with normal saline, and with after filter paper suck dry moisture, accurately weighs liver 100mg, and hepatic glycogen detects Test kit detection Mouse Liver glycogen content.

The mensuration last of 6.4 blood lactase acid is taken a blood sample after being administered 30min, does not the most bear a heavy burden at the water went swimming 10min that temperature is 30 DEG C Rear stopping.Lactic acid instrument assay method: respectively before swimming, each blood sampling 20 μ L add after rest 20min after swimming, after swimming Entering in 40 μ L rupture of membranes liquid, the most fully vibration smudge cells lactic acid instrument measures.(blood lactase acid area under curve=5 × (swimming The blood lactase acid value of 20min after blood lactase acid value+2 × swimming of 0min after front blood lactase acid value+3 × swimming)

7. observation index walking weight load, blood lactase acid, carbamide, glycogen initial value

8. statistical method experimental data is usedRepresent, use t inspection to compare between organizing

9. experimental result

The impact on Mouse Weight of the 9.1 probiotic bacteria honey compositions of the present invention

Each group mice after giving G1～G9 medicine, front, in, shown in post-weight see table respectively, at the beginning of each group mice Initial body weight and weightening finish body weight compare equal no difference of science of statistics (P ＞ 0.05) with matched group, show that G1～G9 medicine is all without significantly Toxicity.Experimental result refers to table 3.

The original body mass of table 3 swimming with a load attached to the body experiment mice, body weight in mid-term and end body weight

The impact on the mice burden swimming time of the 9.2 probiotic bacteria honey compositions of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, can be obviously prolonged mice and bear Weight swimming time, has significant difference (P ＜ 0.05), and probiotic bacteria honey composition G3 of the present invention～G7 medicine are right with blank Compare according to group, can significantly extend the mice burden swimming time, there is pole significant difference (P ＜ 0.01), and be substantially better than G1～ G2 medicine.The results detailed in Table 4.

The impact on the mice burden swimming time of the table 4 probiotic bacteria honey composition

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

9.3 probiotic bacteria honey compositions of the present invention are on the impact of blood lactase acid before and after mouse movement

After per os gives the probiotic bacteria honey composition of the mice present invention, probiotic bacteria honey composition G3 of the present invention～G7 medicine pair After mouse movement, blood lactase acid area under curve compares with matched group significant difference (P ＜ 0.05), G1～G2 medicine group mice Decrease though blood lactase acid area under curve compares with matched group, but and no difference of science of statistics (P ＞ 0.05).The results are shown in Table 5.

Table 5 probiotic bacteria of the present invention honey composition is on the impact of blood lactase acid level before and after mouse movement

" * " p < 0.05vs blank；

The impact on Mouse Liver glycogen of the 9.4 probiotic bacteria honey compositions of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, and Mouse Liver glycogen content all has bright Aobvious rising, have significant difference (P ＜ 0.05), probiotic bacteria honey composition G3 of the present invention～G7 medicine with blank Matched group compares, and Mouse Liver glycogen content all has significantly rising, has pole significant difference (P ＜ 0.01), and the most excellent In G1～G2 medicine.The results detailed in Table 6.

The impact on Mouse Liver glycogen content of the table 6 probiotic bacteria of the present invention honey composition

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

The impact on mice serum carbamide of the 9.5 probiotic bacteria honey compositions of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine group compares with blank group, serum urea after mouse movement Content all has significantly reduction, has significant difference (P ＜ 0.05), probiotic bacteria honey composition G3 of the present invention～G7 medicine Comparing with blank group, after mouse movement, serum urea content all has significantly reduction, has pole significant difference (P ＜ , and be substantially better than G1～G2 medicine 0.01).The results detailed in Table 7.

The impact on mice serum urea content of the table 7 probiotic bacteria of the present invention honey composition

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

10. experiment conclusion

This experiment is mainly tested by mice burden swimming, and the deposit of detection Mouse Liver glycogen observes probiotic bacteria Mel of the present invention simultaneously Compositions improves immunity, the effect of resisting fatigue.Preliminary Results shows as follows:

1, G3～G7 probiotic bacteria honey composition of the present invention all can extend mice burden swimming time (P ＜ 0.01), and effect It is substantially better than the probiotic bacteria honey composition of other G1～G2.

2, biochemistry detection aspect shows, G3～G7 probiotic bacteria honey composition of the present invention each dosage group all can reduce mice after motion Lactic acid content produced by glucose in serum anerobic glycolysis, compares with matched group and has significant difference (P ＜ 0.05), and its Although the probiotic bacteria honey composition of its G1～G2 also can reduce after motion breast produced by glucose anerobic glycolysis in mice serum Acid content, but compare with matched group, no difference of science of statistics (P ＞ 0.05)；

3, G3～G7 probiotic bacteria honey composition of the present invention each dosage group all can significantly improve the deposit (P of glycogen in mouse liver ＜ 0.01), and effect is substantially better than the probiotic bacteria honey composition of other G1～G2；

4, metabolic arthritis model finds, G3～G7 probiotic bacteria honey composition of the present invention can significantly reduce to be urinated in serum after mice is swum The content (P ＜ 0.01) of element, and effect is substantially better than other G1～G2 probiotic bacteria honey composition；

11. conclusions

Above-mentioned experiment proves that probiotic bacteria honey composition of the present invention can significantly improve immunity of organisms, improves muscle power and the endurance of mice, Reduce urea in serum and the content of lactic acid after mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, contribute to delaying Solve the fatigue that sports load causes；The time that mice burden swimming to power exhausts can be extended.

Claims

1. a probiotic bacteria honey composition, is mainly prepared by the raw material of following parts by weight: Mel 155-185 part, Fructus Hordei Germinatus extract 4-10 part, Rhizoma Zingiberis Recens extract 2-6 part, probiotic bacteria powder 1-3 part, fragrant-flowered garlic flower 1-3 part；

Described probiotic bacteria honey composition also includes one or more adjuvants of following parts by weight: Fruit powder 0.6-1.2 part, algae Class 0.5-1 part, aloe vera gel 0.5-1 part, bee pollen 0.2-0.8 part, oligomeric xylose 0.2-0.8 part, oligochitosan 0.1-0.7 part, marine fishbone collagen oligopeptide powder 0.1-0.7 part, Chinese herbal medicine extract 0.1-0.6 part, Cordyceps militaris (L.) Link. 0.1-0.5 Part；

Described probiotic bacteria powder is uniformly mixed by the powder of following parts by weight: Lactobacillus plantarum 30-40 part, the double qi of two qis Bacillus 25-35 part, Bifidobacterium lactis 20-30 part, streptococcus thermophilus 10-20 part, Lactobacillus bulgaricus 12-15 part, addicted to Lactobacillus lactis 12-15 part, lactobacillus casei 8-10 part, lactobacillus rhamnosus 5-10 part.

2. probiotic bacteria honey composition as claimed in claim 1, it is characterised in that Lactobacillus plantarum powder is with Lactobacillus plantarum CGMCC NO.11763 for the bacterium that sets out by preparing according to a conventional method.

3. probiotic bacteria honey composition as claimed in claim 2, it is characterised in that time prepared by described Lactobacillus plantarum powder, freezing is protected Protect the preparation method of agent, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen protein are respectively washed, are drained, press Mass ratio 8-10:3-5:2-4 uniformly mixes, and adds the lactic acid moistening that pH value is 3.8-4.5 of mixed material quality 0.1-1 times 3-8h, pulverizes after-18--22 DEG C of freezing 1-2h immediately, freezing thickness of feed layer 3-5cm, ground product particle diameter 0.5-3mm, It is subsequently added into the water of ground product quality 10-20 times, is 3.5-5.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 25-35kV/cm, burst length 300-500 μ s, carry out high voltage pulse electric field processing under the conditions of pulse frequency 200-300Hz 20-30min；Then under the conditions of power 150-300W, microwave irradiation and extraction 15-20min is carried out in room temperature, simultaneously at power 200-300W, carries out ultrasonic assistant extraction under the conditions of frequency 30-40KHz；Add the compound enzyme of extracting solution quality 1-2%, in 45-55 DEG C of enzymolysis 30-50min；Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that 0.1-0.3mm i.e. obtains protective agent；

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 3-5:2-4:1-3:1-3:1-2 in mass ratio Uniformly mixing.

4. probiotic bacteria honey composition as claimed in claim 3, it is characterised in that described microwave irradiation and extraction is that batch (-type) extracts, I.e. microwave exposure 10s, is spaced 20s.

5. probiotic bacteria honey composition as claimed in claim 1, it is characterised in that the preparation method of described Rhizoma Zingiberis Recens extract, including Following steps: Rhizoma Zingiberis Recens and Dioscorea zingiberensis are respectively put in the ultrasonic washing unit equipped with 0.1-0.3% sodium bicarbonate solution in 200-400W, 30-40KHz clean 5-10min, drain, and 6-9:1-3 in mass ratio uniformly mixes, then cold in-20--24 DEG C Freeze 8-10min；It is crushed to particle diameter 0.2-0.4mm immediately；Put in container and add the water of 4-6 times of weight, obtaining mixed material, using Breast acid for adjusting pH value is 4.5, carries out high-voltage pulse at room temperature under the conditions of electric field intensity 15-25kV/cm, umber of pulse 30-50 Electric field treatment；Then heat to 30-40 DEG C, insulation, under the conditions of power 300-500W, carry out microwave irradiation and extraction, wherein, Every time microwave exposure total time 70s, carry out compartment irradiation: irradiation 10s, be spaced 10s, such irradiation 10 times；Continue to heat up To 40-50 DEG C, insulation, first, in extracting solution, add metal ion so that metal ion content is: sodium ion 28-30mg/L, Zinc ion 20-22mg/L, potassium ion 15-18mg/L, calcium ion 10-12mg/L and magnesium ion 6-8mg/L, be subsequently adding mixed The complex enzyme zymohydrolysis 40-60min of compound material gross weight 0.5-1.5%, Bag filter, obtain filtrate；Filtering residue is by 2 times of weight 60-70 DEG C Water rinses 3 times, and rinsing liquid merges with filtrate, uniformly mixes, and being evaporated to solid content is more than 20%, obtains Rhizoma Zingiberis Recens Extract；

6. probiotic bacteria honey composition as claimed in claim 1, it is characterised in that the preparation method of described Fructus Hordei Germinatus extract is: will Fructus Hordei Germinatus and Fructus Tritici aestivi 8-10:1-3 in mass ratio uniformly mixes, and is crushed to granularity 0.5-1mm, obtains pulverizing Fructus Hordei Germinatus；Then by wood Melon, Fructus Ananadis comosi, Fructus Fici in the ultrasonic washing unit filling 0.3-0.5% sodium bicarbonate solution in power 200W, frequency Ultrasonic cleaning 5-10min under the conditions of 30KHz, drains, and is crushed to granularity 0.5-1mm, and 7-9:1-3:1-2 in mass ratio under room temperature Uniformly mixing, the pulverizing Fructus Hordei Germinatus adding mixture quality 3-5 times obtains raw mixture, adds raw mixture quality 1-3 times Water, is 3-4 with Fructus Citri Limoniae acid for adjusting pH value, carries out microwave extraction under the conditions of power 150-300W, frequency 2000Hz, wherein, Every time microwave exposure total time 60-80s, carry out compartment irradiation: irradiation 10s, be spaced 10s, control temperature 20-35 DEG C, as This irradiation 10 times, simultaneously at power 200-300W, carries out ultrasonic assistant extraction under the conditions of frequency 30-40KHz；Insulation 1-3h, Then, under the conditions of power 200-400W, frequency 2000Hz, carry out microwave extraction, wherein, each microwave exposure total time 90-105s, Carry out compartment irradiation: irradiation 15s, be spaced 10s, control temperature 40-60 DEG C, such irradiation 10 times, simultaneously at power 300-500W, Carry out ultrasonic assistant extraction under the conditions of frequency 40-50KHz, be finally naturally cooling to room temperature, in electric field intensity 25-35kV/cm, Burst length 400-600 μ s, carries out high-pressure pulse electric and extracts 15-20min under the conditions of pulse frequency 200-300Hz；Extracting solution mistake Filter to obtain the first filtrate, add the water rinsing of filtering residue 4 times, filter to obtain the second filtrate, by the first filtrate and the second filtrate 1:3-5 in mass ratio Uniformly mixing, being evaporated to solid content is more than 20%, obtains Fructus Hordei Germinatus extract.

7. probiotic bacteria honey composition as claimed in claim 1, it is characterised in that described Chinese herbal medicine extract is with Bulbus Lilii, gold silver Flower, Herba Menthae, Fructus Momordicae, Colla Corii Asini, Flos Chrysanthemi, Radix Puerariae, Fructus Jujubae, Fructus Cannabis, Semen Nelumbinis, Semen Ziziphi Spinosae, Rhizoma Polygonati, Fructus Lycii, Rhizoma Polygonati Odorati, One or more in the Radix Angelicae Dahuricae, Radix Ginseng, Folium Nelumbinis, Semen Cassiae, Japanese raisintree fruit are prepared for raw material；Preparation method includes walking as follows Rapid: to count by weight, weigh one or more Chinese herbal medicine above-mentioned, put that to fill the ultrasound wave of 0.1-0.3% sodium bicarbonate solution clear Cleaning 10-15min in 200W, 30KHz in washing machine, drain, above-mentioned Chinese medicine powder is broken to particle diameter is below 2mm, puts in container Uniformly mix and add the water of 3-6 times of weight, obtain mixed material, control temperature 70-90 DEG C and keep 2-4h, be then cooled to 40-50 DEG C, it is 3.5-4.5 with breast acid for adjusting pH value, under the conditions of power 150-300W, carries out microwave extraction 10-15min, with Time at power 200-300W, carry out ultrasonic assistant extraction under the conditions of frequency 30-40KHz；It is subsequently adding mixed material gross weight The mixed enzyme of 0.5-1.5% carries out enzymolysis, is 5.5-6.8 with breast acid for adjusting pH value, and enzymolysis 2-4h finally adds mixed material 0.5-3 times of w ethanol and the mixture of propanol, the mass ratio of ethanol and propanol mixing is 1:1-3, controls temperature to 60-78 DEG C Keep 3-4h, filter, obtain the first filtrate；Add the water of 1-3 times of weight of filtering residue, control temperature 85-95 DEG C and keep 1-3h, so After be cooled to 25-35 DEG C, filter, obtain the second filtrate；First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, all Even mixing, obtains Chinese herbal medicine extract；

8. the preparation method of probiotic bacteria honey composition as described in claim 1-7 is arbitrary, it is characterised in that comprise the steps:

9. the preparation method of probiotic bacteria honey composition as claimed in claim 8, it is characterised in that the algae in adjuvant is the raw red ball of rain Any one in algae, salt alga, spirulina plalensis.

10. the preparation method of probiotic bacteria honey composition as claimed in claim 8, it is characterised in that the Fruit powder in adjuvant is Noni fruit Any one in powder, mango powder, pear powder, Fructus Hippophae powder, pueraria root powder.