CN105795300A

CN105795300A - Freeze-dried powder of fruit and vegetable extracts and preparation method of freeze-dried powder

Info

Publication number: CN105795300A
Application number: CN201610204776.4A
Authority: CN
Inventors: 邵素英
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-04-05
Filing date: 2016-04-05
Publication date: 2016-07-27

Abstract

The invention discloses freeze-dried powder of fruit and vegetable extracts. Fruits and vegetables are taken as raw materials, a low-temperature extraction technology is adopted in the whole process, and obtained extracts keep natural color, flavor and taste of the fruits and the vegetables to the greatest extent; an anti-freeze agent which is prepared from natural plant raw materials and has a remarkable cryoprotective effect replaces traditional and existing cryoprotective agents in the vacuum freeze drying process of extracts, the freeze loss caused by a freeze-drying process is further greatly reduced, biological activity and nutritional value of the freeze-dried powder of the extracts are effectively improved, the freeze-dried powder of the extracts is scientifically compounded with functional dietary fiber powder, prebiotic factors and lactobacillus plantarum powder, the medicine and food nutrition balance property as well as functionality, solubility and the probiotic property of the freeze-dried powder of the extracts is remarkably improved, the guarantee period of a product is prolonged, and finally, the freeze-dried powder of fruit and vegetable extracts is prepared, contains more nutritional substances and bioactive substances and is high in function, good in anti-freezing effect and solubility and low in cost.

Description

A kind of fruit and vegerable extract freeze-drying powder and preparation method thereof

Technical field

The present invention relates to the preparation of lyophilized powder, be specifically related to a kind of fruit and vegerable extract freeze-drying powder and preparation method thereof.

Background technology

Lyophilized powder is that medicinal liquid is frozen into solid-state, the aseptic powder that water sublimed is dried by evacuation in an aseptic environment.For dry thermal sensitivity goods and need to keep bioactive material, lyophilizing is a kind of effective method.This method is dry goods will to be needed to make its contained moisture freeze at low temperatures, is then placed under the environment of vacuum dry, and to allow moisture directly be distilled by solid state be steam and gets rid of from goods and makes goods activity dry.The method effectively prevent the change of goods physics and chemistry and biological nature; the damage of biological tissue and cellularity and feature is less; it is made to rapidly enter resting state; effectively protect the stability of many thermal sensitivity medicine biological product active ingredients, as protein, microbiology class degeneration will not occur and lose its biological activity；Secondly, freeze-dried products form after the drying is loose, color does not change substantially, can rapid solution recover physicochemical property and the biological activity of original aqueous solution after adding water；Carry out under vacuum due to dry, for some oxidizable materials, there is good protective effect；Goods water content after lyophilizing is non-normally low, makes the stability of goods improve, and contaminated chance reduces, and this not only facilitates transport and also extends the goods pot-life.

Integration of edible and medicinal herbs " is the wisdom crystallization summed up in food and drug discovery of China working people, embodies food function in health care and treatment.In Ancient Times in China, labourer recognizes that medicine is eaten same merit, in like manner and also had a boundary, and creates this monoid of " medicine-food two-purpose " article.Ancient times are herbal, and especially food book on Chinese herbal medicine works describes substantial amounts of " medicine-food two-purpose " Chinese crude drug, and relative theory, effect, taboo and usage etc. are described in detail, and " medicine-food two-purpose " the kind research for today provides valuable foundation.

No matter from View of International Market or domestic market, health food demand with function is more and more vigorous, the food problem of international and domestic appearance also will raise the integrated demand of health food, the topic that the pursuit to health food is derived constantly is sent with food itself from consumer, have begun to have influence on the overall variation of consuming behavior, including nutraceutical, functional food, Organic food, health food etc..Health industry is no matter from action by government to corporate behavior, just experiencing in the middle of a unprecedented great change, health food will be the Shou Shanzhi district in the middle of this is changed, along with growth in the living standard, people are also more and more higher for the requirement of food, people except require to eat healthy while, it is also desirable to by eating the hope realizing building body.So consumer can conscious go when selecting food to select medicinal and edible food to arrange in pairs or groups with it.And medicinal and edible food price comparison is popular, general people can afford to consume, and the more people of relative health product can select the former, and therefore the prospect of integration of edible and medicinal herbs food is considerable.In a word, medicine is to cure the disease as business, and food is based on health preserving, and you are among us and we are among you, and difficult point each other, and difficulty distinguishes relative superiority or inferiority." integration of edible and medicinal herbs, treating the same disease with different methods, treating different diseases with the same therapeutic principle ".

At present, the more of lyophilized injectable powder is prepared with Chinese crude drug: Chinese patent CN103462911B discloses the technology of preparing of a kind of lyophilized injectable powder.Comprise the steps: that the water for injection of 40%～90% recipe quantity is cooled to 20～60 DEG C, regulate or do not regulate pH, order adds each formulatory agents, stirring, survey pH or adjustment pH, moisturizing, the activated carbon of recipe quantity, stirring decolouring, filter pressing is added under controlled temperature conditions, filtration sterilization obtains sterile liquid medicine, by its subpackage to cillin bottle, and lyophilization, roll lid, obtain lyophilized injectable powder.The method is simple to operate, with short production cycle, is suitable to industrialized production, it is possible to avoid product Yin Gaore to decompose, and avoids between medicine compatible change slowly, gauging of products accurately, good appearance, be conducive to increasing the stability of medicine, it is simple to the long term storage of medicine.Production technology of the present invention is widely used in the preparation of the lyophilized injectable powder of the medicines such as ligustrazine phosphate, sodium ozagrel, levocarnitine, omeprazole.Chinese patent CN103211772B discloses a kind of etoposide lyophilized injectable powder, it is possible to overcome the defect of above-mentioned etoposide, improves etoposide bioavailability, easy to use, absorbs fast, plays a role rapidly.The present invention is by the screening to etoposide injection prescription of freeze-drying powder, find to use specific excipient, and use antioxidant and chelating agen simultaneously, the stability of the injection etoposide freeze-dried powder finished product obtained is high, impurity is few, and side effect is little, and safety is higher, there is convenient storage and use, the advantages such as production cost is low.Chinese patent CN105232478A discloses a kind of aminobutyric acid freeze-dried powder and preparation method thereof, this aminobutyric acid freeze-dried powder is made up of aminobutyric acid, sodium sulfite, mannitol, pH adjusting agent, by the addition of stabilizer, the aminobutyric acid freeze-dried powder end product quality prepared is stable, drug content is high, relevant content of material is low, and restrained effectively the spray phenomenon such as bottle or severe atrophy.

Prepare the less of lyophilized powder with integration of edible and medicinal herbs article for raw material: the preparation method that Chinese patent CN103610141B discloses a kind of freeze-dried ginkgo powder, belong to agricultural product, food processing technology field.It comprises the following steps that: the Semen Ginkgo after broken is put into immersion in clear water, then takes out and drain；Semen Ginkgo after draining carries out microwave ripening；The ginkgo shelling of ripening, remove capsule, then carry out pulverizing, extruding in flakes and removing oil fat；Semen Ginkgo after defat carries out pre-freeze, carries out intensification lyophilizing after pre-freeze；Semen Ginkgo grinds after lyophilizing, namely obtains freeze-dried ginkgo powder.The freeze-dried ginkgo powder harmful components that the present invention obtains are few, lightweight, it is easy to carrying and transport, Semen Ginkgo more concentrates, and nutritional labeling is higher.Chinese patent CN103229920A discloses a kind of Mel lyophilized powder, and it includes Mel, and described Mel is a kind of Powdered lyophilized powder being encapsulated in airtight package container.Described Powdered lyophilized powder can be that in every Powdered lyophilized powder 100 grams described, glucose content is 5 grams to 38 grams, and fructose content is 90 grams to 40 grams, and cane sugar content is less than 3 grams, and enzyme values is more than 9.Adopt the Mel lyophilized powder that the manufacture method of Mel lyophilized powder provided by the invention obtains, utilize the method adding the content that appropriate cold distilled water improves former hydromel, make Mel can form ice shelf structure at low temperatures, thus realizing lyophilization, make to finally give the Mel lyophilized powder of solid state, and be encapsulated in airtight packing container, thus realizing both having preserved the original nutritional labeling of Fresh Honey, long-term preservation can be realized again.Chinese patent CN101199344B discloses " branch is integrated " preparation method of a kind of lyophilized powder producing asparagus ' all-nutrient active matter concentrated juice: squeezed the juice by broken Germinatus Phragmitis tissue, again by the protein enzyme Large molecule active material in juice, process with bioflocculation precipitant, isolate with solid-state " green mud " (A) form again, so as to depart from Germinatus Phragmitis enzyme water soluble substrate, and avoid the impact of degeneration factor (temperature, air, microorganism) in concentration technology.Small molecular in metastable supernatant, is enriched to the concentrated juice (B) of clear and bright micro-Huang through the cryoconcentration of about 10 times.B branch avoids browning reaction because of the addition of flocculating and settling agent and citric acid.A, B bis-branch can individually can also mix after lyophilizing, be namely integrated into end-product lyophilized powder.Chinese patent CN105249129A discloses a kind of blue berry lyophilized powder solid beverage, relate to health beverage processing technique field, beverage consists of: the full fruit jelly dry powder 15-30% of blue berry, flavoring agent (white sugar etc.) white sugar 5%-15%, beverage directly brews with the full fruit jelly dry powder of blue berry for raw material and forms, processing technique mainly includes lyophilizing, packaging and sterilization three process, processing technique is simple, and equipment investment is few, and production cost is low.Relevant process technology is ripe, it is easy to industrialized production.Full fruit pan feeding, low-temperature freeze drying processed, product is of high nutritive value, and health care is good, adapts to market trend.

nullAlthough lyophilized powder has a lot of advantage relative to other powder，But，Vacuum lyophilization process can produce substantial amounts of ice crystal，To animal and plant cells、The organizational structure of product、Quality and basic boom、" the cold quick property " materials such as nutrient substance cell all can cause and stab，And then reduce nutrient substance and bioactive substance titer，Especially the fatality rate of microorganism can be greatly improved in prepared by active microorganism powder，Significantly reduce number of viable，The freeze-dried powder of patent disclosed above whether field of medicaments，Or the lyophilized powder food in integration of edible and medicinal herbs field，All less than improving to solve refrigerating process frost damage prevention problem on the basis of existing vacuum lyophilization，And，Although some pharmaceutical technology field applies cryoprotective agent，But it mostly is poisonous、It is harmful to and chemical substance，It is not applied for food technology field，Simultaneously，Lyophilized powder integration of edible and medicinal herbs raw-food material is single，Poorly soluble、Functional low and not comprehensive.

Chinese patent CN103109930B mono-kind adds the fruity probiotic acid milk slice of sericin antifreeze peptide, its raw material is made up of following component by weight: defatted milk powder 10～14 parts, sucrose 2～5 parts, sericin peptide taken 1～3 part, 1～4 part of fruit material, the active probiotic of lyophilizing 0.1～0.6 part and magnesium stearate 0.1～0.4 part, the preparation method that this invention additionally provides product, technical process includes: the preparation of skimmed milk food stage culture medium, the fermentation of probiotic bacteria, the interpolation of sericin antifreeze peptide, the vacuum lyophilization of fermentation milk, the techniques such as pressed powder, probiotic acid milk slice with the addition of sericin antifreeze peptide and the fruit jelly dry powder rich in vitamin B group, not only make number of live bacteria of probiotics in sour milk tablet high, the bacterium vital preservation time is longer, and improve its taste flavor.Freeze-drying tablet technical development in recent years and clinical advantage are carried out short summary by Qi Yongming, and Zydis technology Problems existing is analyzed, freeze-drying tablet latest Progress is introduced simultaneously, paper freeze drying technology in the history of international and domestic field of medicaments, present situation and development trend, defect that freeze drying technology exists and the technical issues that need to address (Qi Yongming. freeze-drying tablet preparation research new development .JournalofChinaPrescriptionDrug2012.10 (4) .57-59).But, prepare sericin antifreeze peptide cost of material with mulberry cocoon high, cryoprotective effects is single and still not highly desirable.

Adopt fruit and vegerable to prepare expectation and responsibility that the fruit and vegetable lyophilized powder that a kind of nutrient substance and bioactive substance content are high, function is strong, cryoprotective effects good, dissolubility is good, cost is low is those skilled in the art.

Summary of the invention

It is an object of the invention to overcome the defect of existing integration of edible and medicinal herbs food lyophilized powder, with fruit and vegerable for raw material, omnidistance employing extract at low temperature technique, the extract obtained remains the natural colored of fruit and vegerable, local flavor and mouthfeel to greatest extent, make bioactive substance that extract contains and nutraceutical agents content maximize simultaneously, raw material extraction ratio and utilization rate are the highest, reduce extraction cost；Extract adopts the antifreeze with notable cryoprotective effects prepared by natural plant raw material to replace traditional in vacuum lyophilization process, existing cryoprotective agent, greatly reduce the freezing loss that lyophilizing technique causes further, it is effectively increased biological activity and the nutritive value of extract freeze-drying powder, and with functional dietary fiber powder, probiotics factor and Lactobacillus plantarum powder science are composite, the medicine significantly enhancing extract freeze-drying powder eats property balanced in nutrition and functional, dissolubility and probiotic, extend the shelf-life of product, final prepared a kind of nutrient substance and bioactive substance content are high, function is strong, cryoprotective effects and dissolubility are good, the fruit and vegetable lyophilized powder that cost is low.

In order to achieve the above object, the present invention is by the following technical solutions:

A kind of fruit and vegerable extract freeze-drying powder, is mainly prepared by the raw material of following parts by weight:

Fruit and vegerable 30-50 part, food fibre powder 10-30 part, antifreeze 20-30 part, probiotics factor 5-15 part, Lactobacillus plantarum powder 2-10 part；

Preferably, described fruit and vegerable extract freeze-drying powder, mainly prepared by the raw material of following parts by weight:

Fruit and vegerable 35-45 part, food fibre powder 15-25 part, antifreeze 23-27 part, probiotics factor 8-12 part, Lactobacillus plantarum powder 4-8 part；

It is highly preferred that described fruit and vegerable extract freeze-drying powder, mainly prepared by the raw material of following parts by weight:

Fruit and vegerable 40 parts, food fibre powder 20 parts, antifreeze 25 parts, probiotics factor 10 parts, 6 parts of Lactobacillus plantarum powder；

Further, described fruit and vegerable be in Fructus Crataegi, Fructus Lycii, Fructus Jujubae, Fructus Hippophae, Fructus Momordicae, Flos Lonicerae, Poria, Bulbus Lilii, Semen Nelumbinis, Radix Puerariae any one or a few with arbitrary proportion mix.

Further, described food fibre powder is that the soluble fiber cellulose content that dietary fiber is processed through physics, chemical or biological method and obtained is high, biological activity is strong, human body beneficial flora has cellulose important, positive role, compared with full diet fiber, it is functional, biological activity is more powerful；

Preferably, described food fibre powder is to be obtained through micronizing, extruding puffing and enzyme enzymolysis by one or more in inulin, apple fiber, Semen Tritici aestivi fiber, Herba avenae fatuae fiber；

More preferably, the preparation method of described food fibre powder, comprise the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber is 4-6:2-4:1-3 Homogeneous phase mixing in mass ratio, micronizing is to particle diameter 3-6 μm, adjusting ground product moisture is 19-21%, in screw speed 160-180r/min, temperature 185-200 DEG C of condition extruding puffing, add the water of puff quality 2-4 times, room temperature 200-400W, 35-40KHz condition supersound extraction 10-15min, then at electric field intensity 20-40kV/cm, burst length 400-600 μ s, high voltage pulse electric field processing 10-15min is carried out when pulse frequency 200-400Hz；It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Namely enzymolysis solution concentrating under reduced pressure, lyophilization obtain food fibre powder；

Described enzyme is cellulase, xylanase, laccase, pectase, tannase 2-4:1-3:1-2:0.5-1.5:0.2-1 Homogeneous phase mixing in mass ratio.

Further, described antifreeze is the plant extract and trehalose prepared with the natural plants seed containing abundant freeze proof substrate for raw material, sericin peptide taken is composite, fully dissolve and form, the volatile effective components such as the fragrance that both can prevent integration of edible and medicinal herbs raw-food material are taken out of by steam, the ice crystal that can reduce lyophilized powder nutrient substance and bioactive substance again stabs loss, improve the recovery rate of lyophilized powder, add again dissolubility and the stability of lyophilized powder simultaneously, create beyond thought beneficial effect, it is particularly suitable as the freeze drying protectant of integration of edible and medicinal herbs raw-food material extract freeze-drying powder preparation process；

Preferably, the quality group of described antifreeze becomes: plant extract: trehalose: sericin peptide taken=37-53:2-8:1-4；

Preferably, described plant extract be with winter rye, Bulbus Allii Cepae, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici seed in one or more for raw material, through high-pressure electrostatic, high-pressure pulse electric auxiliary bigcatkin willow acid soak, refrigerating process after prepare then through compound enzyme subsection enzymolysis；

More preferably, the preparation method of described plant extract, comprise the steps: by winter rye, Bulbus Allii Cepae, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici seed 13-17:5-11:3-5:1-3 Homogeneous phase mixing in mass ratio, sabot, first in electric field intensity 5-7kV/cm high-pressure electrostatic process 2-4min；Then soaking at room temperature 2-4h in the salicylic acid solution that concentration is 40-60mg/L, simultaneously at electric field intensity 10-20kV/cm, burst length 150-250 μ s, carries out high voltage pulse electric field processing when pulse frequency 150-250Hz；Rinse, drain, 18-24h is stood in 3-5 DEG C, then successively at 1-3 DEG C of cold preservation 2-4d,-3--5 DEG C of freezing 1-3d ,-15--18 DEG C of freezing 20-30h, it is immediately placed at outdoor in intensity of illumination 0.5-5 ten thousand Lx natural lighting 2-4h, makes compound half pulverize immediately after thawing, ground product particle diameter 0.5-1.5mm, it is subsequently added into the water of ground product quality 4-6 times, is 3.5-5.5 with breast acid for adjusting pH value；It is eventually adding the compound enzyme of mixed liquor quality 1-2%, first in 35-50 DEG C of enzymolysis 10-30min, then in 50-60 DEG C of enzymolysis 20-40min；Enzymolysis solution filters, filtrate reduced in volume to solid content is that namely 15-25% obtains plant extract；

Described compound enzyme is cellulase, protease, amylase, pectase, lipase 5-7:2-4:1-3:1-2:1-2 Homogeneous phase mixing in mass ratio.

Further, described probiotics factor weight consists of: oligomeric isomaltose 15-25 part, oligomeric galactose 15-25 part, oligofructose 10-20 part, oligomeric xylose 10-20 part, stachyose 5-11 part, soybean oligo saccharide 5-11 part, Raffinose 4-10 part, Oligomeric maltose 3-7 part.

Further, described Lactobacillus plantarum powder is prepared with Lactobacillus plantarum CGMCCNO.11763 according to a conventional method for starting strain, and its viable bacteria content is: 7 × 10¹²-9×10¹²cfu/g；

Preferably, the cryoprotective agent that time prepared by described Lactobacillus plantarum powder, freeze drying process adopts is antifreeze prepared by the present invention, is remarkably improved Lactobacillus plantarum powder at freezing dry process Viable detection to more than 94%.

It is highly preferred that the mass ratio that Lactobacillus plantarum CGMCCNO.11763 wet thallus mixes with antifreeze is 1:0.6-1.4.

The preparation method that another object of the present invention is to provide above-mentioned fruit and vegerable extract freeze-drying powder, comprises the steps:

null1) preparation of extracting solution: according to formula，Weigh each component raw material，First fruit and vegerable are put in the ultrasonic washing unit equipped with 0.8-1.2% sodium bicarbonate solution in 200-400W、20-40KHz cleans 3-5min，Drain，Pretreatment，Put in microwave drying in power 3-5kW、Thickness of feed layer 2-4cm、50-70℃、Dry 4-6min，Then 10-15min in the sericin peptide taken solution that mass percent is 8-12% it is immersed in,Take out，Pulverize immediately after-18--22 DEG C of freezing 30-50min，Freezing thickness of feed layer 3-5cm，Ground product particle diameter 0.3-0.5mm,It is subsequently added into the water of ground product quality 4-6 times，It is 3.5-5.5 with breast acid for adjusting pH value，At electric field intensity 25-35kV/cm under room temperature，Burst length 300-500 μ s,High voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz；Then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction；Last vacuum outgas obtains fruit and vegetable juice, add the mixed enzyme of its quality 0.2-0.6%, in 40-50 DEG C of enzymolysis 30-50min, take the Ovum Gallus domesticus album of enzymolysis solution quality 0.5-1%, put in blender and stir 10-12min in rotating speed 800-1000r/min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, being added by egg white foams in enzymolysis solution, normal pressure boils 1-3min, is rapidly decreased to room temperature, 80-100 eye mesh screen filters, and filtrate reduced in volume obtains fruit and vegerable extracting solution to solid content to 30-50%；

Described mixed enzyme is cellulase, protease, pectase, lipase 2-4:1-3:1-2:1-2 Homogeneous phase mixing in mass ratio；

2) batch mixing: the 4-6% of antifreeze and food fibre powder quality is sequentially added in extracting solution, Homogeneous phase mixing, at power 300-500W, carry out ultrasonic dissolution 10-15min when frequency 35-45KHz, obtain mixed liquor；

3) lyophilizing: mixed liquor puts in dry storehouse quick-freezing to-40～-50 DEG C, and at-40～-50 DEG C pre-freeze 2～3h；Then after being evacuated to 15～20Pa in dry storehouse, will progressively heat up in 8-10h and control mixed liquor final temperature at-15～-18 DEG C；Then in dry storehouse, vacuum is constant, regulating temperature in dry storehouse makes it progressively be warming up to room temperature in 5-7h, to lyophilized powder moisture less than 4% time discharging, obtain fruit and vegerable extract freeze-drying powder with Lactobacillus plantarum powder, remaining food fibre powder, probiotics factor Homogeneous phase mixing under aseptic condition.

Lactobacillus plantarum of the present invention (Lactobacillusplantarum) XH is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (being called for short CGMCC) on November 30th, 2015, preserving number is CGMCCNO.11763, preservation address is: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.

Lactobacillus plantarum probiotic properties is as follows:

Lactobacillus plantarum CGMCCNO.11763 provided by the present invention is found to survive when pH is 1.50 through experiment, still in existing state after 1% cholate is cultivated 4 hours；Lactobacillus plantarum CGMCCNO.11763 degrading nitrite speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at below 4.8mg/kg；Degrading rate of cholesterol, after fermentation 60h hour, can be reached 64.76% by CGMCCNO.11763.CGMCCNO.11763 Adhering capacity measure from coagulation rate be 95.71%.

Lactobacillus plantarum CGMCCNO.11763 is to cholesterol degradation capability study and mensuration:

Take 1mlCGMCCNO.11763 mother solution and be inoculated in MRS cholesterol fluid medium (the cholesterol level 0.1mg/ml of 10mL, pH6.2) in, the constant temperature of 37 DEG C stands and cultivates 20h respectively, 40h, 60h is standby, with the MRS cholesterol culture medium that accesses 1mL sterilized water for comparison, take bacteria liquid sample and the comparison each 1ml of liquid of above cultivation different time, 9000r/min, centrifugal 10min at 4 DEG C, obtain fermented supernatant fluid, in o-phthalaldehyde method mensuration supernatant, cholesterol level is (particularly as follows: take each supernatant 0.1ml in corresponding test tube, add glacial acetic acid 0.3ml, the o-phthalaldehyde(OPA) 0.15ml of 1mg/ml, it is slowly added into concentrated sulphuric acid 1.0ml, mix homogeneously.Room temperature stands 10min, surveys light absorption value under 550nm).Each process 3 repetition, in kind makes cholesterol standard curve, calculates cholesterol level and degradation rate in supernatant, and result is in Table 1.It can be seen that cholesterol is had good Degradation by CGMCCNO.11763, after fermentation 60h hour, degradation rate can reach 64.76%.

The table 1 degraded situation to cholesterol.

Degradation time (h)	0	20h	40h	60h
					Cholesterol level (mg/ml)	0.2273±0.0058	0.1356±0.0018	0.1011±0.0094	0.801±0.0231
Degrading rate of cholesterol %		40.34%	55.52%	64.76%

The bile tolerance test of Lactobacillus plantarum CGMCCNO.11763 bacterial strain:

Take CGMCCNO.11763 bacterium solution 1mL and inoculate strain in the 10mLMRS fluid medium (PH=6.4) containing different cholate (concentration gradients is 0.0%, 0.2%, 0.4%, 0.6%, 0.8%, 1%), be placed at 37 DEG C to cultivate respectively 0,2,4h, each process 3 repetition.Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilution factor solution, take 0.1ml diluent to be coated with in MRS, be inverted in 37 DEG C of biochemical cultivation cases and cultivate 48 hours (each dilution factor do 3 parallel) record and calculate the several number of bacterium on flat board.Result is in Table 2.This bacterium known increment of bacterium after gallbladder salinity is 1% process 4h still reaches 0.59 ± 0.92 × 10⁷(cfu/ml), there is good bile tolerance ability.

Table 2 bile tolerance ability detection [(± s) × 10⁷cfu/ml]

The acid resistance test of Lactobacillus plantarum CGMCCNO.11763 bacterial strain

Take HLX37 mother solution and inoculate strain in the 10mLMRS fluid medium of different pH value (pH gradient is 1.5,2.0,2.5,3.0,3.5,4.0) by 1ml, be placed at 37 DEG C to cultivate respectively 0,2,4h, each process 3 repetition.Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilute solution, take 0.1ml diluent and be coated with in MRS, in 37 DEG C of biochemical cultivation cases, be inverted the bacterium colony number cultivated on 48 hours (each dilution factor do 3 parallel) record flat board.Result is in Table 3.Illustrate that this bacterium has very strong acid-fast ability.

Table 3 acid-fast ability detection [(± s) × 10⁷cfu/ml]

The Adhering capacity of Lactobacillus plantarum CGMCCNO.11763 measures

Cultivate CGMCCNO.11763 (MRS fluid medium), bacillus coli DH 5 alpha (LB fluid medium) 24h obtains fermentation liquid, be respectively placed in 3000r/min, centrifugal 10min at 4 DEG C, collect bacterium mud, wash bacterium mud 2 times with the sterile phosphate buffer (PBS) of pH=7.0 respectively and (in bacterium colony, namely add PBS, after concussion mix homogeneously, be placed in 3000r/min, centrifugal 10min at 4 DEG C, collect thalline).From coagulation rate (%): with aseptic PBS, bacterium mud CGMCCNO.11763 to be formed in the suspension bacteria liquid that light absorption value is 0.4 ± 0.1 (A0) and the bacteria suspension at wavelength 600nm place, measure light absorption value A24 after standing 24h, be (A0 A24)/A0 from coagulation rate (%) formula.；His coagulation rate (%): the outstanding bacterium solution of CGMCCNO.11763 and bacillus coli DH 5 alpha is adjusted to the mix suspending bacterium solution that the light absorption value at wavelength 600nm place is 0.6 ± 0.1 (A0).Measuring light absorption value A24 after standing 24H, his coagulation rate (%) formula is (A0 A24)/A0.Measurement result is in Table 5, it is known that CGMCCNO.11763 is 95.71% from coagulation rate, has very strong Adhering capacity.

Table 4 Adhering capacity table

The bacterial strain physiological property of Lactobacillus plantarum CGMCCNO.11763

Described Lactobacillus plantarum (Lactobacillusplantarum) XH is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (being called for short CGMCC) on November 30th, 2015, preserving number is CGMCCNO.11763, preservation address is: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.

This bacterial strain feature is as follows: examine under a microscope, and this bacterial strain is rod-short, and Gram’s staining is positive, atrichia, does not produce spore；On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circular, and edge is more neat.

Physicochemical characteristics is: catalase (-), gelatin liquefaction (-), indole experiment (+), mobility (-), fermentation gas (-), nitrate reductase (-), fermentation gas (-), product hydrogen sulfide gas (-), pH4.0MRS culture medium grows (+).It is accredited as Lactobacillus plantarum (Lactobacillusplantarum), called after Lactobacillus plantarum (Lactobacillusplantarum) XH through Physiology and biochemistry.

Bacterial strain can at 57 DEG C well-grown, glucose tolerance is 275g/L.

Lactobacillus plantarum of the present invention, by gathering people Li Jianshu, separates in Yoghourt from Xinjiang Uygur fellow-villager family and obtains, acquisition time on June 2nd, 2015.

Lactobacillus plantarum CGMCCNO.11763 of the present invention is found to survive when pH is 1.50 through experiment, still in existing state after 1% cholate is cultivated 4 hours；Lactobacillus plantarum CGMCCNO.11763 degrading nitrite speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at below 4.8mg/kg；Degrading rate of cholesterol, after fermentation 60h hour, can be reached 64.76% by CGMCCNO.11763.CGMCCNO.11763 Adhering capacity measure from coagulation rate be 95.71%, bacterial strain can at 57 DEG C well-grown, glucose tolerance is 275g/L.

Beneficial effect:

Fruit and vegerable, with fruit and vegerable for raw material, are carried out parasite killing initially with ultrasonic cleaning and go out ovum, killing microorganisms, removal pesticide residues and heavy metal ion etc., substantially increase the foodsafety of lyophilized powder by the present invention；Microwave drying is adopted to make fruit and vegerable in short-term, mainly enzyme denaturing, fixation while low-temperature explosion-puffing drying, remain the natural colored of fruit and vegerable to greatest extent, fruit and vegerable natural colored after microwave color fixing is highly stable in the follow-up course of processing, will not variable color, fade, color and luster is very bright-coloured, can keep the natural colored of fruit and vegerable；Aqueous solution containing sericin peptide taken soaks rehydration, chilled and the fruit and vegerable active substance that causes and cold quick property composition loss can be reduced to greatest extent, improve the extraction ratio of fruit and vegerable effective ingredient, omnidistance employing extract at low temperature technique, by ultrasonic cleaning, microwave deactivating enzyme fixation and expanded, high-pressure pulse electric extracts, ultrasonic wave added microwave extraction, biological enzymolysis and vacuum outgas organically combine, the extract obtained remains the natural colored of fruit and vegerable to greatest extent, local flavor and mouthfeel, make bioactive substance that extract contains and nutraceutical agents content maximize simultaneously, raw material extraction ratio and utilization rate are the highest, reduce extraction cost；Adopt egg white foams prepared by Ovum Gallus domesticus album to enzymolysis solution flocks in short-term, further increase dissolubility and the stability of lyophilized powder；Part food fibre powder is added in extracting solution and antifreeze Homogeneous phase mixing, the solid content of batch mixing can be improved, reduce moisture, alleviate the burden of freezing process, shorten freeze-drying time, improve lyophilizing efficiency, through ultrasonic dissolution, further enhancing the homogeneity of batch mixing, stability and dissolubility, and then improve the homogeneity of lyophilized powder, stability and dissolubility；Extract adopts the antifreeze with notable cryoprotective effects prepared by natural plant raw material to replace traditional in vacuum lyophilization process, existing cryoprotective agent, greatly reduce the freezing loss that lyophilizing technique causes further, it is effectively increased biological activity and the nutritive value of extract freeze-drying powder, and it is composite with functional dietary fiber powder and Lactobacillus plantarum powder science, the medicine significantly enhancing extract freeze-drying powder eats property balanced in nutrition and functional, dissolubility and probiotic, extend the shelf-life of product, final prepared a kind of nutrient substance and bioactive substance content are high, function is strong, cryoprotective effects and dissolubility are good, the fruit and vegetable lyophilized powder that cost is low.Concrete test effect is shown in embodiment 7-13, and concrete know-why is as follows:

1. the Lactobacillus plantarum powder Viable detection of the present invention is high, function is complete, probiotic by force, and Lactobacillus plantarum CGMCCNO.11763 is found to survive when pH is 1.50 through experiment, still in existing state after 1% cholate is cultivated 4 hours；Lactobacillus plantarum CGMCCNO.11763 degrading nitrite speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at below 4.8mg/kg；Degrading rate of cholesterol, after fermentation 60h hour, can be reached 64.76% by CGMCCNO.11763.CGMCCNO.11763 Adhering capacity measure from coagulation rate be 95.71%.

null2. the antifreeze that prepared by the present invention is the plant extract and trehalose prepared with the natural plants seed containing abundant freeze proof substrate for raw material、Sericin peptide taken is composite to be formed，Without any chemical substance and food additive，Natural freeze proof substrate is comprehensive、Abundant，Antifreeze protein and antifreeze peptide content are high，Substantially increase the foodsafety of lyophilized powder，The volatile effective components such as the fragrance that both can prevent integration of edible and medicinal herbs raw-food material are taken out of by steam，The ice crystal that can reduce lyophilized powder nutrient substance and bioactive substance again stabs loss，Improve the recovery rate of lyophilized powder，Add again dissolubility and the stability of lyophilized powder simultaneously，Create beyond thought beneficial effect，It is particularly suitable as the freeze drying protectant of integration of edible and medicinal herbs raw-food material extract freeze-drying powder preparation process，Existing cryoprotective agent can be substituted completely，And produce better effect；Meanwhile, it is applied to the freeze-drying process of Lactobacillus plantarum powder, is remarkably improved Viable detection to more than 94%.Plant extract therein is by composite for the natural plants seed feed science containing abundant freeze proof substrate, high-pressure electrostatic is processed, high-pressure pulse electric auxiliary Induced by Salicylic Acid, low temperature segmentation Stress treatment and natural lighting organically combine, make coercing and inducing down of active seed boundary's environment outside containing freeze proof substrate itself, freeze proof matrix components obtains the most comprehensive, the abundantest synthesis and accumulation, maximizing dissolution after biological enzymolysis, antifreeze peptide content can be made to increase simultaneously, improve dissolubility and the stability of plant extract, and then eliminate the problem that the lyophilized powder caused because of antifreeze is poorly soluble, with food fibre powder, Lactobacillus plantarum powder, probiotics factor science is composite, effect is more preferably.

3. the food fibre powder that prepared by the present invention is by micronizing, extruding puffing, supersound extraction, high-pressure pulse electric extracts and biological enzymolysis combination of sciences, gained food fibre powder retentiveness, dilatancy, thickening property is higher, the modified dietary fiber prepared than single method improves 20-40%, and acid and alkali, alkali, the impact of salt, soluble fiber cellulose content is high, the modified dietary fiber prepared than single method improves 10-30%, it is easier to be utilized by lactic acid bacteria, improve lactic acid bacteria in the growth of human body intestinal canal and fertility, increase kind and the quantity of probiotic bacteria flora, reduce human body intestinal canal pH value, improve human body intestinal canal microecological environment；High adsorption capacity, after modified, cellulosic specific surface area increases, and network is enriched, and absorption affinity strengthens, and the organic molecule ability of chelating, absorption cholesterol and bile acids is higher, suppress the human body absorption to them；Ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is higher, effectively prevent body weight for humans metal poisoning；Regulate and maintain the resident time of intestinal microbial population, strengthen digestion and the absorbability of intestinal, improve immunity of organisms；Effectively facilitate gastrointestinal peristalsis, slow down and eliminate the untoward reaction such as flatulence, abdominal distention；Powerful embedding effect can prevent environment (oxygen, temperature, illumination, the water activity etc.) factor impact on lyophilized powder quality, stabilizes the biological activity of lyophilized powder further, extends the shelf-life of lyophilized powder.

4. the preparation method of lyophilized powder of the present invention adopts non-thermal technology technology; work efficiency is high; energy resource consumption is few; environmentally friendly, pollution-free, decrease the introducing of food additive and chemical assistant; it is effectively increased foodsafety; improve product quality and reduce production cost, it is achieved that low-carbon (LC) productive target, can scale, industrialized development.Microwave drying particularly: 1) is adopted to make fruit and vegerable in short-term, mainly enzyme denaturing, fixation while low-temperature explosion-puffing drying, remain the natural colored of fruit and vegerable to greatest extent, fruit and vegerable natural colored after microwave color fixing is highly stable in the follow-up course of processing, will not variable color, fade, color and luster is very bright-coloured, can keep the natural colored of fruit and vegerable；2) in the aqueous solution containing sericin peptide taken, rehydration is soaked, chilled and the fruit and vegerable active substance that causes and cold quick property composition loss can be reduced to greatest extent, improve the extraction ratio of fruit and vegerable effective ingredient, omnidistance employing extract at low temperature technique, by ultrasonic cleaning, microwave deactivating enzyme fixation and expanded, high-pressure pulse electric extracts, ultrasonic wave added microwave extraction, biological enzymolysis and vacuum outgas organically combine, the extract obtained remains the natural colored of fruit and vegerable to greatest extent, local flavor and mouthfeel, make bioactive substance that extract contains and nutraceutical agents content maximize simultaneously, raw material extraction ratio and utilization rate are the highest, reduce extraction cost；3) adopt egg white foams prepared by Ovum Gallus domesticus album to enzymolysis solution flocks in short-term, further increase dissolubility and the stability of lyophilized powder；4) by extracting solution appropriateness concentrating under reduced pressure, it is subsequently adding part food fibre powder and antifreeze Homogeneous phase mixing, the solid content of batch mixing can be improved, reduce moisture, alleviate the burden of freezing process, shorten freeze-drying time, improve lyophilizing efficiency, through ultrasonic dissolution, further enhancing the homogeneity of batch mixing, stability and dissolubility, and then improve the homogeneity of lyophilized powder, stability and dissolubility, improve the lyophilizing rate of freeze-drying process.

It should be noted that the eating effect of this lyophilized powder is the result that each component is mutually worked in coordination with, interacted, the not superposition of simple raw material function, the science of each raw material components is composite and extracts, the effect produced, considerably beyond the superposition of each single component function and effect, has advanced preferably and practicality.

Detailed description of the invention

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, under the premise without departing substantially from spirit and scope of the present invention, the various changes or the changes that carry out the material component in these embodiments and consumption fall within protection scope of the present invention.

Prepared by embodiment 1 raw material

1. the preparation of food fibre powder

The preparation method of described food fibre powder, comprise the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber 5:3:2 Homogeneous phase mixing in mass ratio, micronizing is to particle diameter 5 μm, adjusting ground product moisture is 20%, in screw speed 170r/min, 192 DEG C of condition extruding puffings of temperature, add the water of puff quality 3 times, room temperature 300W, 40KHz condition supersound extraction 12min, then at electric field intensity 30kV/cm, burst length 500 μ s, carries out high voltage pulse electric field processing 12min when pulse frequency 300Hz；It is 5.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.2%, in 50 DEG C of enzymolysis 35min；Namely enzymolysis solution concentrating under reduced pressure, lyophilization obtain food fibre powder；

Described enzyme is cellulase, xylanase, laccase, pectase, tannase 3:2:1.5:1:0.6 Homogeneous phase mixing in mass ratio.

2. the preparation of plant extract

The preparation method of described plant extract, comprise the steps: by winter rye, Bulbus Allii Cepae, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici seed 15:8:4:2 Homogeneous phase mixing in mass ratio, sabot, first in electric field intensity 6kV/cm high-pressure electrostatic process 3min；Then soaking at room temperature 3h in the salicylic acid solution that concentration is 50mg/L, simultaneously at electric field intensity 15kV/cm, burst length 200 μ s, carries out high voltage pulse electric field processing when pulse frequency 200Hz；Rinse, drain, 21h is stood in 4 DEG C, then successively at 2 DEG C of cold preservation 3d,-4 DEG C of freezing 2d ,-16 DEG C of freezing 25h, it is immediately placed at outdoor in intensity of illumination 30,000 Lx natural lighting 3h, makes compound half pulverize immediately after thawing, ground product particle diameter 1.0mm, it is subsequently added into the water of ground product quality 5 times, is 4.5 with breast acid for adjusting pH value；It is eventually adding the compound enzyme of mixed liquor quality 1.5%, first in 42 DEG C of enzymolysis 20min, then in 55 DEG C of enzymolysis 30min；Enzymolysis solution filters, filtrate reduced in volume to solid content is 20% namely obtain plant extract；

Described compound enzyme is cellulase, protease, amylase, pectase, lipase 6:3:2:1.5:1.5 Homogeneous phase mixing in mass ratio.

Food fibre powder, plant extract that example 2 below-6 uses are embodiment 1 and prepare.

Embodiment 2

A kind of wolfberry fruit extract lyophilized powder, is mainly prepared by the raw material of following parts by weight:

Fructus Lycii 40 parts, food fibre powder 20 parts, antifreeze 25 parts, probiotics factor 10 parts, 6 parts of Lactobacillus plantarum powder；

The quality group of described antifreeze becomes: plant extract: trehalose: sericin peptide taken=45:5:2；

Described probiotics factor weight consists of: oligomeric isomaltose 20 parts, oligomeric galactose 20 parts, oligofructose 15 parts, oligomeric xylose 15 parts, 8 parts of stachyose, soybean oligo saccharide 8 parts, Raffinose 7 parts, Oligomeric maltose 5 parts；

Described Lactobacillus plantarum powder is prepared with Lactobacillus plantarum CGMCCNO.11763 according to a conventional method for starting strain, and its viable bacteria content is: 9 × 10¹²cfu/g；Wherein, the cryoprotective agent that freeze drying process adopts is above-mentioned antifreeze prepared by the present invention, and the mass ratio that wet thallus mixes with antifreeze is 1:1.

Preparation method, comprises the steps:

1) preparation of extracting solution: according to formula, weigh each component raw material, first Fructus Lycii is put in the ultrasonic washing unit equipped with 1% sodium bicarbonate solution in 300W, 30KHz cleans 4min, drain, put in microwave drying in power 4kW, thickness of feed layer 3cm, 60 DEG C, dry 5min, then 12min in the sericin peptide taken solution that mass percent is 10% it is immersed in, take out, pulverize immediately after-20 DEG C of freezing 40min, freezing thickness of feed layer 4cm, ground product particle diameter 0.4mm, it is subsequently added into the water of ground product quality 5 times, it is 4.5 with breast acid for adjusting pH value, at electric field intensity 30kV/cm under room temperature, burst length 400 μ s, high voltage pulse electric field processing 25min is carried out when pulse frequency 250Hz；Then carry out microwave irradiation and extraction 18min when power 200W in room temperature, simultaneously at power 250W, when frequency 35KHz, carry out ultrasonic assistant extraction；Last vacuum outgas obtains fruit and vegetable juice, add the mixed enzyme of its quality 0.4%, in 45 DEG C of enzymolysis 40min, take the Ovum Gallus domesticus album of enzymolysis solution quality 0.8%, put in blender and stir 11min in rotating speed 900r/min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, being added by egg white foams in enzymolysis solution, normal pressure boils 2min, is rapidly decreased to room temperature, 90 eye mesh screens filter, and filtrate reduced in volume obtains fructus lycii extracted solution to solid content to 40%；

Described mixed enzyme is cellulase, protease, pectase, lipase 3:2:1.5:1.5 Homogeneous phase mixing in mass ratio；

2) batch mixing: be sequentially added in extracting solution by the 5% of antifreeze and food fibre powder quality, Homogeneous phase mixing, at power 400W, carry out ultrasonic dissolution 12min when frequency 40KHz, obtain mixed liquor；

3) lyophilizing: mixed liquor puts in dry storehouse quick-freezing to-45 DEG C, and at-45 DEG C pre-freeze 2.5h；Then after being evacuated to 18Pa in dry storehouse, will progressively heat up in 9h and control mixed liquor final temperature at-17 DEG C；Then in dry storehouse, vacuum is constant, regulating temperature in dry storehouse makes it progressively be warming up to room temperature in 6h, to lyophilized powder moisture less than 4% time discharging, obtain wolfberry fruit extract lyophilized powder with Lactobacillus plantarum powder, remaining food fibre powder, probiotics factor Homogeneous phase mixing under aseptic condition.

Embodiment 3

A kind of Fructus Jujubae extract lyophilized powder, is mainly prepared by the raw material of following parts by weight:

Fructus Jujubae 35 parts, food fibre powder 15 parts, antifreeze 23 parts, probiotics factor 8 parts, 4 parts of Lactobacillus plantarum powder；

The quality group of described antifreeze becomes: plant extract: trehalose: sericin peptide taken=37:2:1；

Described probiotics factor weight consists of: oligomeric isomaltose 15 parts, oligomeric galactose 15 parts, oligofructose 10 parts, oligomeric xylose 10 parts, 5 parts of stachyose, soybean oligo saccharide 5 parts, Raffinose 4 parts, Oligomeric maltose 3 parts；

Described Lactobacillus plantarum powder is prepared with Lactobacillus plantarum CGMCCNO.11763 according to a conventional method for starting strain, and its viable bacteria content is: 8 × 10¹²cfu/g；Wherein, the cryoprotective agent that freeze drying process adopts is above-mentioned antifreeze prepared by the present invention, and the mass ratio that wet thallus mixes with antifreeze is 1:0.6.

Preparation method, comprises the steps:

null1) preparation of extracting solution: according to formula，Weigh each component raw material，First Fructus Jujubae is put in the ultrasonic washing unit equipped with 0.8% sodium bicarbonate solution in 200W、20KHz cleans 3min，Drain，Enucleation、Section，Put in microwave drying in power 3kW、Thickness of feed layer 2cm、50℃、Dry 4min，Then 10min in the sericin peptide taken solution that mass percent is 8% it is immersed in,Take out，Pulverize immediately after-18 DEG C of freezing 30min，Freezing thickness of feed layer 3cm，Ground product particle diameter 0.3mm,It is subsequently added into the water of ground product quality 4 times，It is 3.5 with breast acid for adjusting pH value，At electric field intensity 25kV/cm under room temperature，Burst length 300 μ s,High voltage pulse electric field processing 20min is carried out when pulse frequency 200Hz；Then carry out microwave irradiation and extraction 15min when power 150W in room temperature, simultaneously at power 200W, when frequency 30KHz, carry out ultrasonic assistant extraction；Last vacuum outgas obtains fruit and vegetable juice, add the mixed enzyme of its quality 0.2%, in 40 DEG C of enzymolysis 30min, take the Ovum Gallus domesticus album of enzymolysis solution quality 0.5%, put in blender and stir 10min in rotating speed 800r/min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, being added by egg white foams in enzymolysis solution, normal pressure boils 1min, is rapidly decreased to room temperature, 80 eye mesh screens filter, and filtrate reduced in volume obtains jujube extract to solid content to 30%；

Described mixed enzyme is cellulase, protease, pectase, lipase 2:1:1:1 Homogeneous phase mixing in mass ratio；

2) batch mixing: be sequentially added in extracting solution by the 4% of antifreeze and food fibre powder quality, Homogeneous phase mixing, at power 300W, carry out ultrasonic dissolution 10min when frequency 35KHz, obtain mixed liquor；

3) lyophilizing: mixed liquor puts in dry storehouse quick-freezing to-40 DEG C, and at-40 DEG C pre-freeze 2h；Then after being evacuated to 15Pa in dry storehouse, will progressively heat up in 8h and control mixed liquor final temperature at-15 DEG C；Then in dry storehouse, vacuum is constant, regulating temperature in dry storehouse makes it progressively be warming up to room temperature in 5h, to lyophilized powder moisture less than 4% time discharging, obtain Fructus Jujubae extract lyophilized powder with Lactobacillus plantarum powder, remaining food fibre powder, probiotics factor Homogeneous phase mixing under aseptic condition.

Embodiment 4

A kind of Fructus Crataegi extract lyophilized powder, is mainly prepared by the raw material of following parts by weight:

Fructus Crataegi 45 parts, food fibre powder 25 parts, antifreeze 27 parts, probiotics factor 12 parts, 8 parts of Lactobacillus plantarum powder；

The quality group of described antifreeze becomes: plant extract: trehalose: sericin peptide taken=53:8:4；

Described probiotics factor weight consists of: oligomeric isomaltose 25 parts, oligomeric galactose 25 parts, oligofructose 20 parts, oligomeric xylose 20 parts, 11 parts of stachyose, soybean oligo saccharide 11 parts, Raffinose 10 parts, Oligomeric maltose 7 parts；

Described Lactobacillus plantarum powder is prepared with Lactobacillus plantarum CGMCCNO.11763 according to a conventional method for starting strain, and its viable bacteria content is: 7 × 10¹²cfu/g；Wherein, the cryoprotective agent that freeze drying process adopts is above-mentioned antifreeze prepared by the present invention, and the mass ratio that wet thallus mixes with antifreeze is 1:1.4.

Preparation method, comprises the steps:

null1) preparation of extracting solution: according to formula，Weigh each component raw material，First Fructus Crataegi is put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution in 400W、40KHz cleans 5min，Drain，Pretreatment，Put in microwave drying in power 5kW、Thickness of feed layer 4cm、70℃、Dry 6min，Then 15min in the sericin peptide taken solution that mass percent is 12% it is immersed in,Take out，Pulverize immediately after-22 DEG C of freezing 50min，Freezing thickness of feed layer 5cm，Ground product particle diameter 0.5mm,It is subsequently added into the water of ground product quality 6 times，It is 5.5 with breast acid for adjusting pH value，At electric field intensity 35kV/cm under room temperature，Burst length 500 μ s,High voltage pulse electric field processing 30min is carried out when pulse frequency 300Hz；Then carry out microwave irradiation and extraction 20min when power 300W in room temperature, simultaneously at power 300W, when frequency 40KHz, carry out ultrasonic assistant extraction；Last vacuum outgas obtains fruit and vegetable juice, add the mixed enzyme of its quality 0.6%, in 50 DEG C of enzymolysis 50min, take the Ovum Gallus domesticus album of enzymolysis solution quality 1%, put in blender and stir 12min in rotating speed 1000r/min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, being added by egg white foams in enzymolysis solution, normal pressure boils 3min, is rapidly decreased to room temperature, 100 eye mesh screens filter, and filtrate reduced in volume obtains hawthorn extract to solid content to 50%；

Described mixed enzyme is cellulase, protease, pectase, lipase 4:3:2:2 Homogeneous phase mixing in mass ratio；

2) batch mixing: be sequentially added in extracting solution by the 6% of antifreeze and food fibre powder quality, Homogeneous phase mixing, at power 500W, carry out ultrasonic dissolution 15min when frequency 45KHz, obtain mixed liquor；

3) lyophilizing: mixed liquor puts in dry storehouse quick-freezing to-50 DEG C, and at-50 DEG C pre-freeze 3h；Then after being evacuated to 20Pa in dry storehouse, will progressively heat up in 10h and control mixed liquor final temperature at-18 DEG C；Then in dry storehouse, vacuum is constant, regulating temperature in dry storehouse makes it progressively be warming up to room temperature in 7h, to lyophilized powder moisture less than 4% time discharging, obtain Fructus Crataegi extract lyophilized powder with Lactobacillus plantarum powder, remaining food fibre powder, probiotics factor Homogeneous phase mixing under aseptic condition.

Embodiment 5

A kind of Flos Lonicerae extract lyophilized powder, is mainly prepared by the raw material of following parts by weight:

Flos Lonicerae 30 parts, food fibre powder 10 parts, antifreeze 20 parts, probiotics factor 5 parts, 2 parts of Lactobacillus plantarum powder；

The quality group of described antifreeze becomes: plant extract: trehalose: sericin peptide taken=37:8:1；

Described probiotics factor weight consists of: oligomeric isomaltose 15 parts, oligomeric galactose 25 parts, oligofructose 10 parts, oligomeric xylose 20 parts, 5 parts of stachyose, soybean oligo saccharide 11 parts, Raffinose 4 parts, Oligomeric maltose 7 parts；

Described Lactobacillus plantarum powder is prepared with Lactobacillus plantarum CGMCCNO.11763 according to a conventional method for starting strain, and its viable bacteria content is: 9 × 10¹²cfu/g；Wherein, the cryoprotective agent that freeze drying process adopts is antifreeze prepared by the present invention, and the mass ratio that wet thallus mixes with antifreeze is 1:0.8.

Preparation method, comprises the steps:

null1) preparation of extracting solution: according to formula，Weigh each component raw material，First Flos Lonicerae is put in the ultrasonic washing unit equipped with 0.8% sodium bicarbonate solution in 400W、20KHz cleans 5min，Drain，Section，Put in microwave drying in power 3kW、Thickness of feed layer 4cm、50℃、Dry 6min，Then 15min in the sericin peptide taken solution that mass percent is 8% it is immersed in,Take out，Pulverize immediately after-18 DEG C of freezing 50min，Freezing thickness of feed layer 3cm，Ground product particle diameter 0.5mm,It is subsequently added into the water of ground product quality 4 times，It is 5.5 with breast acid for adjusting pH value，At electric field intensity 25kV/cm under room temperature，Burst length 500 μ s,High voltage pulse electric field processing 30min is carried out when pulse frequency 200Hz；Then carry out microwave irradiation and extraction 20min when power 150W in room temperature, simultaneously at power 200W, when frequency 40KHz, carry out ultrasonic assistant extraction；Last vacuum outgas obtains fruit and vegetable juice, add the mixed enzyme of its quality 0.2%, in 50 DEG C of enzymolysis 30min, take the Ovum Gallus domesticus album of enzymolysis solution quality 1%, put in blender and stir 12min in rotating speed 800r/min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, being added by egg white foams in enzymolysis solution, normal pressure boils 1min, is rapidly decreased to room temperature, 100 eye mesh screens filter, and filtrate reduced in volume obtains Flos Lonicerae extractive solution to solid content to 30%；

Described mixed enzyme is cellulase, protease, pectase, lipase 2:3:1:2 Homogeneous phase mixing in mass ratio；

2) batch mixing: be sequentially added in extracting solution by the 4% of antifreeze and food fibre powder quality, Homogeneous phase mixing, at power 300-500W, carry out ultrasonic dissolution 10min when frequency 45KHz, obtain mixed liquor；

3) lyophilizing: mixed liquor puts in dry storehouse quick-freezing to-40 DEG C, and at-50 DEG C pre-freeze 2h；Then after being evacuated to 20Pa in dry storehouse, will progressively heat up in 8h and control mixed liquor final temperature at-18 DEG C；Then in dry storehouse, vacuum is constant, regulating temperature in dry storehouse makes it progressively be warming up to room temperature in 5h, to lyophilized powder moisture less than 4% time discharging, obtain Flos Lonicerae extract lyophilized powder with Lactobacillus plantarum powder, remaining food fibre powder, probiotics factor Homogeneous phase mixing under aseptic condition.

Embodiment 6

A kind of Fructus Crataegi, Fructus Jujubae extract lyophilized powder, mainly prepared by the raw material of following parts by weight:

Fructus Crataegi 30 parts, Fructus Jujubae 20 parts, food fibre powder 30 parts, antifreeze 30 parts, probiotics factor 15 parts, 10 parts of Lactobacillus plantarum powder；

The quality group of described antifreeze becomes: plant extract: trehalose: sericin peptide taken=53:2:4；

Described probiotics factor weight consists of: oligomeric isomaltose 25 parts, oligomeric galactose 15 parts, oligofructose 20 parts, oligomeric xylose 10 parts, 11 parts of stachyose, soybean oligo saccharide 5 parts, Raffinose 10 parts, Oligomeric maltose 3 parts.

Described Lactobacillus plantarum powder is prepared with Lactobacillus plantarum CGMCCNO.11763 according to a conventional method for starting strain, and its viable bacteria content is: 9 × 10¹²cfu/g；Wherein, the cryoprotective agent that freeze drying process adopts is antifreeze prepared by the present invention, and the mass ratio that wet thallus mixes with antifreeze is 1:1.2.

Preparation method, comprises the steps:

null1) preparation of extracting solution: according to formula，Weigh each component raw material，First by Fructus Crataegi、Fructus Jujubae is respectively put in the ultrasonic washing unit equipped with 0.8-1.2% sodium bicarbonate solution in 400W、20KHz cleans 5min，Drain，Enucleation、Section，Homogeneous phase mixing，Put in microwave drying in power 5kW、Thickness of feed layer 2cm、70℃、Dry 4min，Then 10min in the sericin peptide taken solution that mass percent is 12% it is immersed in,Take out，Pulverize immediately after-22 DEG C of freezing 30min，Freezing thickness of feed layer 5cm，Ground product particle diameter 0.3mm,It is subsequently added into the water of ground product quality 6 times，It is 3.5 with breast acid for adjusting pH value，At electric field intensity 35kV/cm under room temperature，Burst length 300 μ s,High voltage pulse electric field processing 20min is carried out when pulse frequency 300Hz；Then carry out microwave irradiation and extraction 15min when power 300W in room temperature, simultaneously at power 300W, when frequency 30KHz, carry out ultrasonic assistant extraction；Last vacuum outgas obtains fruit and vegetable juice, add the mixed enzyme of its quality 0.6%, in 40 DEG C of enzymolysis 50min, take the Ovum Gallus domesticus album of enzymolysis solution quality 0.5%, put in blender and stir 10min in rotating speed 1000r/min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, being added by egg white foams in enzymolysis solution, normal pressure boils 3min, is rapidly decreased to room temperature, 80 eye mesh screens filter, and filtrate reduced in volume obtains Fructus Crataegi, jujube extract to solid content to 50%；

Described mixed enzyme is cellulase, protease, pectase, lipase 4:1:2:1 Homogeneous phase mixing in mass ratio；

2) batch mixing: be sequentially added in extracting solution by the 5% of antifreeze and food fibre powder quality, Homogeneous phase mixing, at power 500W, carry out ultrasonic dissolution 15min when frequency 35KHz, obtain mixed liquor；

3) lyophilizing: mixed liquor puts in dry storehouse quick-freezing to-50 DEG C, and at-40 DEG C pre-freeze 3h；Then after being evacuated to 15Pa in dry storehouse, will progressively heat up in 10h and control mixed liquor final temperature at-15 DEG C；Then in dry storehouse, vacuum is constant, regulating temperature in dry storehouse makes it progressively be warming up to room temperature in 7h, to lyophilized powder moisture less than 4% time discharging, obtain Fructus Crataegi, Fructus Jujubae extract lyophilized powder with Lactobacillus plantarum powder, remaining food fibre powder, probiotics factor Homogeneous phase mixing under aseptic condition.

Embodiment 7 fruit and vegerable extract freeze-drying powder dissolubility test of the present invention

Lyophilized powder is as a kind of powder mainly prepared by integration of edible and medicinal herbs raw-food material, stability criterion with reference to solid beverage, clarity after dissolution time, dissolving is defined as the quality stability index of lyophilized powder, it not only directly reflects the dissolution degree of product, also reflect product inherent quality and stability, be the most important quality control index of lyophilized powder.

Dissolution time: 1:5 adds 55-65 DEG C of warm water in mass ratio, is sufficiently stirred for, the consoluet time；

Clarity characterizing method: clear 90-100 divides；Micro-turbid 80-89 divides；Muddy 70-79 divides；More muddy 60-69 divides；Serious muddy 50-59 divides；Precipitate less than 49 points (deposit seed is more big, quantity is more many, and mark is more low).

The wolfberry fruit extract lyophilized powder embodiment of the present invention 2 prepared and commercially available same type, same specification, wolfberry fruit extract lyophilized powder with the date of manufacture stand, observe clarity after dissolving in glass, and once, result is in Table 1 for every 5min observed and recorded result:

Table 1: lyophilized powder clarity testing result after dissolving

Result above shows: lyophilized powder of the present invention is compared with commercially available lyophilized powder, dissolubility is better, and dissolution time is short, and the stability of solution after dissolving is strong, clarity is high, presentation quality is better, and along with the prolongation of time of repose, commercially available lyophilized powder solution liquid phase is relatively unstable, produce precipitation, leveraging presentation quality and the inherent quality of beverage, also illustrate that lyophilized powder inherent quality of the present invention is better simultaneously, eating effect is safe and stable.

It should be understood that fruit and vegerable extract freeze-drying powder prepared by embodiment of the present invention 3-6 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect diversity.

Embodiment 8 eats the change of T-CHOL after fruit and vegerable extract freeze-drying powder

Selecting the adult 48 of T-CHOL 180mg/dl-250mg/dl, men and women half and half, is randomly divided into three groups；200 milliliters of mineral waters are drunk in first group of dinner every day；Second group of common commercially available wolfberry fruit extract lyophilized powder 40g of dinner every day Instant Drinks, 1:5 adds 55-65 DEG C of warm water in mass ratio, is sufficiently stirred for, is completely dissolved；The wolfberry fruit extract lyophilized powder 80g of the 3rd group of dinner every day Instant Drinks embodiment of the present invention 2,1:5 adds 55-65 DEG C of warm water in mass ratio, is sufficiently stirred for, is completely dissolved；Every day period eats same food, and food includes meat, egg, vegetable and fruit.Respectively at the blood of the previous day that experiment starts and the 20th, 40,60 days acquisition test persons, measuring the total cholesterol level in blood, result is table 2 such as:

Table 2: total cholesterol level testing result in blood

Time	0 day	20 days	40 days	60 days
					First group (mg/dl)	202.3	203.8	206.2	209.3
Second group (mg/dl)	207.6	206.5	203.2	202.1
					3rd group (mg/dl)	209.8	201.9	190.5	168.3

As seen from the above table after the wolfberry fruit extract lyophilized powder of the Instant Drinks embodiment of the present invention 2, the content generation significant change of the T-CHOL in adult's blood.Compared with common commercially available wolfberry fruit extract lyophilized powder, the content of the T-CHOL in adult's blood is significantly reduced by wolfberry fruit extract lyophilized powder of the present invention, and the content of the T-CHOL in mineral water composition year human blood significantly increases, although commercially available wolfberry fruit extract lyophilized powder decreases, but compared with product of the present invention, effect is notable, it follows that the present invention adopts lyophilized powder prepared by the specific bacterial strain with reduction cholesterol characteristic to have the health-care effect well reducing cholesterol.

It should be understood that the fruit and vegerable extract freeze-drying powder prepared by embodiment of the present invention 3-6 has above-mentioned technique effect equally, between each embodiment, diversity is not notable.

Embodiment 9 fruit and vegerable extract freeze-drying powder shelf-life implants living preparation of lactobacillus stable content test of the present invention

Take the wolfberry fruit extract lyophilized powder of the embodiment of the present invention 2 preparation in room temperature 22-25 DEG C, store 3 months, 6 months, 12 months, 24 months, 36 months respectively and measure Lactobacillus plantarum viable bacteria content under ventilation condition, result is table 3 such as:

Table 3: shelf-life implants living preparation of lactobacillus content detection result

Result above shows: the storage stability of lyophilized powder of the present invention is fabulous, environment resistant (cold and hot temperature, humidity, oxygen, illumination, moisture etc.) influence factor's ability is big, shelf-life implants living preparation of lactobacillus content loss rate maximum (36 months) is 10%, higher than existing like product viable bacteria content, loss rate is low, long shelf-life, reflects that other bioactive ingredients of lyophilized powder has same storage stability simultaneously.

The sensory evaluation test of embodiment 10 fruit and vegerable extract freeze-drying powder of the present invention

The wolfberry fruit extract lyophilized powder that the embodiment of the present invention 2 is prepared by 24 personnel is invited to judge with the lyophilized powder of commercially available two kinds of similar identical dates of manufacture, sense organ is given a mark, wherein specialty and each 12 of layman, professional is young, middle aged, each 4 of old age, men and women half and half, layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half；Marking includes outward appearance (20 points), quality (25 points), local flavor (30 points), four aspects of mouthfeel (25 points), and marking personnel independently carry out, and are independent of each other, and judges result with guarantee accurate.Having added up judging result, equal score value takes approximation, retains integer, is specifically shown in table 4:

Table 4: sensory evaluation statistical result

Note: show significant difference (P < 0.05) with the different lowercase alphabet of mark in a line, the different capitalization of mark represents difference extremely notable (P < 0.01), indicates same letter and represents difference not notable (P > 0.05).

Result above shows, any one will be substantially better than commercially available lyophilized powder to lyophilized powder prepared by the present invention from outward appearance, quality, local flavor and mouthfeel, particularly outward appearance, local flavor and mouthfeel are fabulous, remain the natural colored of integration of edible and medicinal herbs raw-food material, taste and flavor to greatest extent, also be adapted for different age group, the consumer of different hierarchy of consumption eats simultaneously.

The embodiment 11 fruit and vegerable of the present invention probiotic test of extract freeze-drying powder

The wolfberry fruit extract lyophilized powder embodiment of the present invention 2 prepared, preparing into viable count with sterilized water is 2 × 10¹⁰The Lactobacillus plantarum solution of CFU/mL, saves backup in 4 DEG C；

1, the 10mL Lactobacillus plantarum solution kept of going bail for is injected in test tube 1, adopts ten times of stepwise dilutions to 10^-8, take 1mL diluent on flat board, the MRS agar culture medium being cooled to 45 DEG C poured on flat board (sterilizing), shake up rapidly after sterilizing.Will be equipped with the test tube 2 of 10mL Lactobacillus plantarum solution again to be placed in 80-90 DEG C of water-bath and heat 15-25min, take the Lactobacillus plantarum solution after heating and carry out ten times of stepwise dilutions to 10^-8, take 1mL diluent on flat board, the MRS agar culture medium being cooled to 45 DEG C poured into upper at flat board (sterilizing) and shake up rapidly after sterilizing.Finally the flat board before heating and after heating is all cultivated under 35 DEG C of conditions 24h, calculates the quantity before and after heating.

It is shown that Viable detection has reached more than 88%.

2, the resistance test of simulated gastric fluid and intestinal juice: the hydrochloric acid 16.4mL taking 100g/L adds distilled water diluting, make pH value respectively 1.5,2.5 and 3.5, take 100mL dilute hydrochloric acid solution, it is separately added into 1g pepsin, it is made fully to dissolve, obtaining simulated gastric fluid, microporous filter membrane degerming (0.22 μm) is standby.Taking potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, regulates pH value to 6.8 with 0.1moL/L sodium hydroxide solution；Separately taking trypsin 10g, the 100mL that adds water makes dissolving, after two liquid mixing, is diluted with water to 1000ml, obtains simulated intestinal fluid, and microporous filter membrane degerming (0.22 μm) is standby.Take the 1mL Lactobacillus plantarum solution kept to join in the simulated gastric fluid of 9mL (i.e. ten times of stepwise dilutions), and fully mix on the oscillator rapidly, be subsequently placed in 30-45 DEG C of quiescent culture 2-4h.Take out culture fluid respectively when 1h, 2h, 3h, 4h and count remaining viable count immediately, comparing with former viable count, it is shown that Viable detection is 98%.Then each 1mL of the culture fluid digesting different time it is taken in simulated gastric fluid, it is inoculated in the simulated intestinal fluid that 9mLpH value is 6.8 respectively, it is placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h sampling, measure its viable count, comparing with former viable count, result shows that Viable detection is 99%.

3, simulating the resistance test of cholate: make the solution of 1g/L with pancreatin, and add the Fel Sus domestica salt of 0.8% in the solution, the NaOH adjustment pH with 10% is 8.0, then with 0.45 μm of micro-filtrate membrane filtration degerming.The Lactobacillus plantarum solution inoculum kept by 0.5mL is simulated in cholate to 4.5mL, obtains culture fluid, the viable count of counting remaining after cultivating 24h.By culture fluid in sterile saline ten times of stepwise dilutions to 10^-8, and pour into MRS, it is subsequently placed in 35 DEG C of quiescent culture 24h.Result shows that Viable detection is 99%.

Above result of the test shows, the Lactobacillus plantarum probiotic (thermostability, resistance to pH, bile tolerance) in lyophilized powder of the present invention is relatively strong, is especially suitable for human intestines and stomach's environment, and in simulated gastrointestinal environments, survival rate is big, can be effectively improved gastrointestinal function.

Embodiment 12 fruit and vegerable of the present invention extract freeze-drying powder mouse intestinal performance test

Choosing common Kunming white mice 60, male and female half and half, 18-20g, conventional word is supported.Therefrom random choose 40,9:00 gavage lincomycin hydrochloride 0.2mL every morning (20mg)/only, other as a control group, every day same time gavage equivalent sterile saline, continuous one week, prepare the mouse model of alteration of intestinal flora.Model group mouse diet declines, and dead and phenomenon of significantly suffering from diarrhoea does not occur, arranges soft excrement, and profile normal aqueous divides more, and bedding and padding are moist.By 40 alteration of intestinal flora mices, being randomly divided into 2 groups, one group 20 is only used as treatment group, the Lactobacillus plantarum solution 0.5ml (2 × 10 that every day, gavage was kept¹⁰Cfu/ml)/only, another 20 are only used as natural recovering group, every day same time gavage equivalent sterile saline, continuous two weeks.21 days whole experimental periods, observing growth and the defecation situation of white mice every day, weigh in the 8th, 21 days mices to lyophilized powder treatment group and natural recovering group, calculate each group of weight average rate of increase, result is table 5 such as；Within every 5 days, surveying each group of stool in mice escherichia coli quantity, calculate average, result is table 6 such as.Take stool in mice and be about 0.1g, in aseptic operating platform, add 3 beades (adding 0.5mL diluent with 0.1g excrement sample), dilute and inoculate maconkey agar culture medium, calculate every gram of coliform count wet in just.

Table 5: mice Gain weight

Packet	Average starting weight (g/ is only)	Average end weight (g/ is only)	Average rate of increase (%)
				Natural recovering group	20.69±1.33	27.34±1.59	32.14^a
Treatment group	20.41±1.45	34.28±1.62	67.96^b

Table 6: the situation of coliform count in stool in mice

Lyophilized powder treatment group Mouse Weight average rate of increase (67.96%) is significantly higher than natural recovering group (32.14%)；Feed solution rear intestinal escherichia coli quantity to be remarkably decreased, reduce by 97.07%, it is substantially less than natural recovering group (24.78%), show the rapid field planting in white mice intestinal of the Lactobacillus plantarum in lyophilized powder of the present invention, form dominant microflora, and effectively suppress the growth and breeding of the pathogen such as escherichia coli, and resident time is long, continues, effectively improves intestinal performance.

The impact on immunity of organisms of the embodiment 13 fruit and vegerable extract freeze-drying powder of the present invention

1 experiment purpose

Test (mouse forced swimming) by exercise tolerance, verify the raising immunity of fruit and vegerable extract freeze-drying powder of the present invention, antifatigue effect.

2 experiment materials and reagent

2.1 for reagent thing:

Commercially available fruit and vegerable extract freeze-drying powder (G1)；Commercially available fruit and vegerable extract freeze-drying powder (G2)；Fruit and vegerable extract freeze-drying powder (G3-G7) prepared by embodiment of the present invention 2-6.

2.2 reagent:

Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing；Concentrated sulphuric acid (AR), Nanjing Chemistry Reagent Co., Ltd.；Normal saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..

3. laboratory animal

ICR mice, ♂, cleaning grade, body weight 18-22g, Ningxia Medical University's comparative medicine center provide, the free diet of mice during experiment.

4. key instrument

Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof company；Water-bath: DK-S26 type, the grand experimental facilities company limited of upper Nereid；Electronic scale: BS224S type, Sartorius company；Stopwatch, thermometer

5. experiment packet

5.1 dosage packets and given the test agent give the time and at random mice are divided into 8 groups, often group 10,1st group to the 7th group medicine giving G1～G7 respectively, 8th group is blank group, give isopyknic distilled water, the often every average daily gavage of group 1 time, gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.

5.2 sample preparations the 1st group to the 7th group: weigh 2.25g drug sample, be assigned to 150ml with distilled water；Blank group: distilled water 150ml.

6. experimental technique

After 6.1 swimming with a load attached to the body experiment last administration 30min, putting mice in swimming trunk, the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of rat-tail root load 5% body weight, and record mice swimming started to the dead time, as mice swimming time.

After 6.2 mice serum carbamide measure last administration 30min, not swimming with a load attached to the body 90min in the water that temperature is 30 DEG C, eyeball blood sampling 0.5mL (being not added with anticoagulant) is plucked after rest 60min, put 4 DEG C of refrigerator 3h, after hemopexis, the centrifugal 15min of 2000r/min, takes serum and send clinical laboratory of Affiliated Hospital of Ningxia Medical University to detect.

After the mensuration last administration 30min of 6.3 hepatic glycogen, not swimming with a load attached to the body 90min in the water that temperature is 25 ± 1 DEG C, cervical dislocation puts to death mice, clean with normal saline, and with, after filter paper suck dry moisture, accurately weighing liver 100mg, hepatic glycogen detection kit detection Mouse Liver glycogen content.

Blood sampling after the mensuration last administration 30min of 6.4 blood lactase acid, does not then bear a heavy burden and stops after the water went swimming 10min that temperature is 30 DEG C.Lactic acid instrument assay method: respectively before swimming, each blood sampling 20 μ L add in 40 μ L rupture of membranes liquid after rest 20min after swimming, after swimming, fully vibration smudge cells lactic acid instrument measures immediately.(blood lactase acid area under curve=5 × (after front blood lactase acid value+3 × swimming of swimming the blood lactase acid value of 20min after blood lactase acid value+2 × swimming of 0min)

7. observation index walking weight load, blood lactase acid, carbamide, glycogen initial value

8. statistical method experimental data is usedRepresent, adopt t inspection to compare between organizing

9. experimental result

The impact on Mouse Weight of the 9.1 fruit and vegerable extract freeze-drying powders of the present invention

Each group mice is after giving G1～G9 medicine, before, in, post-weight is shown in shown in following table respectively, and each original body mass organizing mice and weightening finish body weight compare equal no difference of science of statistics (P > 0.05) with matched group, it was shown that G1～G9 medicine is all without obvious toxicity.Experimental result refers to table 7.

The original body mass of table 7 swimming with a load attached to the body experiment mice, mid-term body weight and terminate body weight

The impact on the mice burden swimming time of the 9.2 fruit and vegerable extract freeze-drying powders of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, the mice burden swimming time can be obviously prolonged, there is significant difference (P < 0.05), fruit and vegerable extract freeze-drying powder G3～G7 medicine of the present invention compares with blank group, can significantly extend the mice burden swimming time, there is pole significant difference (P < 0.01), and be substantially better than G1～G2 medicine.The results detailed in Table 8.

The impact on the mice burden swimming time of the table 8 fruit and vegerable extract freeze-drying powder

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

9.3 fruit and vegerable extract freeze-drying powders of the present invention are on the impact of blood lactase acid before and after mouse movement

After per os gives the fruit and vegerable extract freeze-drying powder of the mice present invention, blood lactase acid area under curve after mouse movement is compared by fruit and vegerable extract freeze-drying powder G3～G7 medicine of the present invention with matched group significant difference (P < 0.05), decrease though G1～G2 medicine group Mouse Blood lactic acid area under curve compares with matched group, but and no difference of science of statistics (P > 0.05).Result is in Table 9.

Table 9 fruit and vegerable extract freeze-drying powder of the present invention is on the impact of blood lactase acid level before and after mouse movement

" * " p < 0.05vs blank；

The impact on Mouse Liver glycogen of the 9.4 fruit and vegerable extract freeze-drying powders of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, Mouse Liver glycogen content all has obvious rising, there is significant difference (P < 0.05), fruit and vegerable extract freeze-drying powder G3～G7 medicine of the present invention compares with blank group, Mouse Liver glycogen content all has obvious rising, has pole significant difference (P < 0.01), and is substantially better than G1～G2 medicine.The results detailed in Table 10.

The impact on Mouse Liver glycogen content of the table 10 fruit and vegerable extract freeze-drying powder of the present invention

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

The impact on mice serum carbamide of the 9.5 fruit and vegerable extract freeze-drying powders of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine group compares with blank group, after mouse movement, serum urea content all has obvious reduction, there is significant difference (P < 0.05), fruit and vegerable extract freeze-drying powder G3～G7 medicine of the present invention compares with blank group, after mouse movement, serum urea content all has obvious reduction, has pole significant difference (P < 0.01), and is substantially better than G1～G2 medicine.The results detailed in Table 11.

The impact on mice serum urea content of the table 11 fruit and vegerable extract freeze-drying powder of the present invention

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

10. experiment conclusion

This experiment is tested mainly through mice burden swimming, and the deposit simultaneously detecting Mouse Liver glycogen observes the effect of fruit and vegerable extract freeze-drying powder of the present invention raising immunity, resisting fatigue.Preliminary Results shows below:

1, G3～G7 fruit and vegerable extract freeze-drying powder of the present invention all can extend the mice burden swimming time (P < 0.01), and effect is substantially better than the fruit and vegerable extract freeze-drying powder of other G1～G2.

2, biochemistry detection aspect shows, the each dosage group of G3～G7 fruit and vegerable extract freeze-drying powder of the present invention all can reduce move after lactic acid content produced by glucose anerobic glycolysis in mice serum, compare with matched group and have significant difference (P < 0.05), and although the fruit and vegerable extract freeze-drying powder of other G1～G2 also can reduce after motion lactic acid content produced by glucose anerobic glycolysis in mice serum, but compare with matched group, no difference of science of statistics (P > 0.05)；

3, each dosage group of G3～G7 fruit and vegerable extract freeze-drying powder of the present invention all can significantly improve the deposit (P < 0.01) of glycogen in mouse liver, and effect is substantially better than the fruit and vegerable extract freeze-drying powder of other G1～G2；

4, metabolic arthritis model finds, G3～G7 fruit and vegerable extract freeze-drying powder of the present invention can significantly reduce the content (P < 0.01) of urea in serum after mice is swum, and effect is substantially better than other G1～G2 fruit and vegerable extract freeze-drying powder；

11. conclusion

Above-mentioned experiment proves that fruit and vegerable extract freeze-drying powder of the present invention can significantly improve immunity of organisms, improve muscle power and the endurance of mice, the content of urea in serum and lactic acid after reduction mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, contribute to alleviating the fatigue that sports load causes；The time that mice burden swimming to power exhausts can be extended.

Claims

1. a fruit and vegerable extract freeze-drying powder, is mainly prepared by the raw material of following parts by weight: fruit and vegerable 30-50 part, food fibre powder 10-30 part, antifreeze 20-30 part, probiotics factor 5-15 part, Lactobacillus plantarum powder 2-10 part；

Described antifreeze is that the plant extract prepared for raw material with the natural plants seed containing freeze proof substrate forms with trehalose, sericin peptide taken dissolving composite, abundant；

The quality group of described antifreeze becomes: plant extract: trehalose: sericin peptide taken=37-53:2-8:1-4.

2. fruit and vegerable extract freeze-drying powder as claimed in claim 1, it is characterised in that mainly prepared by the raw material of following parts by weight: fruit and vegerable 35-45 part, food fibre powder 15-25 part, antifreeze 23-27 part, probiotics factor 8-12 part, Lactobacillus plantarum powder 4-8 part.

3. fruit and vegerable extract freeze-drying powder as claimed in claim 1, it is characterised in that mainly prepared by the raw material of following parts by weight: fruit and vegerable 40 parts, food fibre powder 20 parts, antifreeze 25 parts, probiotics factor 10 parts, 6 parts of Lactobacillus plantarum powder.

null4. the fruit and vegerable extract freeze-drying powder as described in as arbitrary in claim 1-3，It is characterized in that，The preparation method of described food fibre powder，Comprise the following steps: by inulin、Apple fiber、Semen Tritici aestivi fiber is 4-6:2-4:1-3 Homogeneous phase mixing in mass ratio，Micronizing is to particle diameter 3-6 μm，Adjusting ground product moisture is 19-21%，In screw speed 160-180r/min、Temperature 185-200 DEG C of condition extruding puffing，Add the water of puff quality 2-4 times，Room temperature 200-400W、35-40KHz condition supersound extraction 10-15min,Then at electric field intensity 20-40kV/cm，Burst length 400-600 μ s,High voltage pulse electric field processing 10-15min is carried out when pulse frequency 200-400Hz；It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Namely enzymolysis solution concentrating under reduced pressure, lyophilization obtain food fibre powder；

5. the fruit and vegerable extract freeze-drying powder as described in as arbitrary in claim 1-3, it is characterized in that, the preparation method of described plant extract, comprise the steps: by winter rye, Bulbus Allii Cepae, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici seed 13-17:5-11:3-5:1-3 Homogeneous phase mixing in mass ratio, sabot, first processes 2-4min in electric field intensity 5-7kV/cm high-pressure electrostatic；Then soaking at room temperature 2-4h in the salicylic acid solution that concentration is 40-60mg/L, simultaneously at electric field intensity 10-20kV/cm, burst length 150-250 μ s, carries out high voltage pulse electric field processing when pulse frequency 150-250Hz；Rinse, drain, 18-24h is stood in 3-5 DEG C, then successively at 1-3 DEG C of cold preservation 2-4d,-3--5 DEG C of freezing 1-3d ,-15--18 DEG C of freezing 20-30h, it is immediately placed at outdoor in intensity of illumination 0.5-5 ten thousand Lx natural lighting 2-4h, makes compound half pulverize immediately after thawing, ground product particle diameter 0.5-1.5mm, it is subsequently added into the water of ground product quality 4-6 times, is 3.5-5.5 with breast acid for adjusting pH value；It is eventually adding the compound enzyme of mixed liquor quality 1-2%, first in 35-50 DEG C of enzymolysis 10-30min, then in 50-60 DEG C of enzymolysis 20-40min；Enzymolysis solution filters, filtrate reduced in volume to solid content is that namely 15-25% obtains plant extract；

6. the fruit and vegerable extract freeze-drying powder as described in as arbitrary in claim 1-3, it is characterised in that described Lactobacillus plantarum powder is prepared with Lactobacillus plantarum CGMCCNO.11763 according to a conventional method for starting strain, and its viable bacteria content is: 7 × 10¹²-9×10¹²cfu/g。

7. fruit and vegerable extract freeze-drying powder as claimed in claim 6, it is characterised in that the mass ratio that time prepared by described Lactobacillus plantarum powder, wet thallus mixes with antifreeze is 1:0.6-1.4.

8. the preparation method of fruit and vegerable extract freeze-drying powder as described in as arbitrary in claim 1-7, it is characterised in that comprise the steps:

9. the preparation method of fruit and vegerable extract freeze-drying powder as claimed in claim 8, it is characterized in that, described probiotics factor weight consists of: oligomeric isomaltose 15-25 part, oligomeric galactose 15-25 part, oligofructose 10-20 part, oligomeric xylose 10-20 part, stachyose 5-11 part, soybean oligo saccharide 5-11 part, Raffinose 4-10 part, Oligomeric maltose 3-7 part.

10. the preparation method of fruit and vegerable extract freeze-drying powder as claimed in claim 8, it is characterized in that, described fruit and vegerable be in Fructus Crataegi, Fructus Lycii, Fructus Jujubae, Fructus Hippophae, Fructus Momordicae, Flos Lonicerae, Poria, Bulbus Lilii, Semen Nelumbinis, Radix Puerariae any one or a few with arbitrary proportion mix.