CN106360720A - Instant medicinal and edible food extract freeze-dried powder and preparation method thereof - Google Patents

Instant medicinal and edible food extract freeze-dried powder and preparation method thereof Download PDF

Info

Publication number
CN106360720A
CN106360720A CN201610743227.4A CN201610743227A CN106360720A CN 106360720 A CN106360720 A CN 106360720A CN 201610743227 A CN201610743227 A CN 201610743227A CN 106360720 A CN106360720 A CN 106360720A
Authority
CN
China
Prior art keywords
powder
edible
medicinal herbs
food
integration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610743227.4A
Other languages
Chinese (zh)
Inventor
邵素英
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beijing Kehuitong Intelligent Technology Co ltd
Original Assignee
Beijing Kehuitong Intelligent Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Kehuitong Intelligent Technology Co ltd filed Critical Beijing Kehuitong Intelligent Technology Co ltd
Publication of CN106360720A publication Critical patent/CN106360720A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/169—Plantarum

Landscapes

  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention discloses an instant medicinal and edible food extract freeze-dried powder, which takes medicinal and edible food as raw materials, adopts a low-temperature extraction process in the whole process, and the obtained extract furthest retains the natural color, flavor and taste of the medicinal and edible food; the antifreeze agent with obvious antifreeze effect prepared from natural plant raw materials is used for replacing the traditional and existing cryoprotectant in the vacuum freeze drying process of the extract, so that the freezing loss caused by the freeze-drying process is further greatly reduced, the biological activity and the nutritional value of the extract freeze-dried powder are effectively improved, the extract freeze-dried powder is compounded with functional dietary fiber powder, probiotic factors and lactobacillus plantarum powder, the medicine-food nutrition balance, the functionality, the solubility and the probiotic of the extract freeze-dried powder are obviously enhanced, the quality guarantee period of the product is prolonged, and finally the instant medicine-food homologous food extract freeze-dried powder with high content of nutrient substances and bioactive substances, strong function, good antifreeze effect and solubility and low cost is prepared.

Description

A kind of instant integration of edible and medicinal herbs food extracts lyophilized powder and preparation method thereof
Technical field
The present invention relates to the preparation of lyophilized powder is and in particular to a kind of instant integration of edible and medicinal herbs food extracts lyophilized powder and its system Preparation Method.
Background technology
Lyophilized powder is in an aseptic environment medicinal liquid to be frozen into solid-state, and evacuation is aseptic by water sublimed drying Powder.For thermal sensitivity product being dried and needing to keep the material of biological activity, lyophilizing is a kind of effective method.This method is need to Product to be dried makes its contained moisture freeze at low temperature, is dried, allows moisture by solid in the environment of being then placed on vacuum State directly rises Huawei's vapor and excludes from product and so that product activity is dried.The method effectively prevent product physics and chemistry And the change of biological nature, the damage to biological tissue and cellularity and feature less so as to rapidly enter resting state, have Effect protects the stability of many thermal sensitivity medicine biological product active ingredients, and such as protein, microbiology class will not occur degeneration With its biological activity of loss；Secondly, form is loose after the drying, color does not change substantially for freeze-dried products, can after adding water Quickly dissolve and recover physicochemical property and the biological activity of original aqueous solution；Because drying is carried out under vacuum, for one Oxidizable material has good protective effect a bit；Product water content after lyophilizing is very low, makes the stability of product Improve, contaminated chance reduces, this not only facilitates transport and also extends the product pot-life.
Integration of edible and medicinal herbs " is the wisdom crystallization that China working people summarizes in food and drug discovery, embodies food and exists Health care and the function for the treatment of aspect.In Ancient Times in China, labourer recognizes that medicine is eaten same work(, had boundary in the same manner and also, and creates " Medicine-food two-purpose " this monoid of article.Ancient times book on Chinese herbal medicine, especially food book on Chinese herbal medicine works, describe in substantial amounts of " medicine-food two-purpose " Medical material, and relative theory, effect, taboo and usage etc. are described in detail, it is that " medicine-food two-purpose " kind research of today provides Valuable foundation.
No matter from View of International Market or domestic market, carry functional health food demand more and more vigorous, international state The food problem of interior appearance also will raise the integrated demand of health food, from consumer constantly send the pursuit to health food with From the point of view of the topic that food is derived in itself, have begun to have influence on the overall variation of consuming behavior, including nutraceutical, function Food, Organic food, health food etc..Health industry no matter from action by government to corporate behavior, just experiencing one unprecedented Great change in the middle of, health food will be this change in the middle of Shou Shanzhi area, with growth in the living standard, people are for food The requirement of product also more and more higher, people except while requiring to eat healthy it is also desirable to by eating the hope to realize building body Hope.So consumer can conscious go to select medicinal and edible food to arrange in pairs or groups therewith when selecting food.And medicine food The food price of homology is more popular, and general people can consume and must rise, before for health product, more people can select relatively Person, the prospect of therefore integration of edible and medicinal herbs food is considerable.In a word, to cure the disease as business, based on health preserving, you are part of me for food for medicine, I In have you, difficult point is each other, and difficulty distinguishes relative superiority or inferiority." integration of edible and medicinal herbs, treating the same disease with different methods, treating different diseases with the same therapeutic principle ".
At present, the more of lyophilized injectable powder is prepared with Chinese crude drug: Chinese patent cn 103462911 b discloses a kind of jelly The technology of preparing of dry powder injection.Comprise the steps: that the water for injection of 40%～90% recipe quantity is cooled to 20～60 DEG C, adjust Or do not adjust ph, sequentially add each formulatory agents, stirring, survey ph or regulation ph, moisturizing, under controlled temperature conditions, add the activity of recipe quantity Charcoal, stirring decolouring, filter pressing, filtration sterilization obtains sterile liquid medicine, by its subpackage to cillin bottle, lyophilization, roll lid, obtain final product lyophilizing Injectable powder.The method is simple to operate, with short production cycle, is suitable to industrialized production, product can be avoided to decompose because of hyperpyrexia, and keep away Exempt from slow compatible change between medicine, gauging of products is accurate, good appearance, be conducive to increasing the stability of medicine, be easy to medicine Long term storage.Production technology of the present invention is widely used in ligustrazine phosphate, sodium ozagrel, levocarnitine, omeprazole etc. The preparation of the lyophilized injectable powder of medicine.Chinese patent cn 103211772 b discloses a kind of etoposide lyophilized injectable powder, energy Enough overcome the defect of above-mentioned etoposide, improve etoposide bioavailability, easy to use, absorb fast, play a role rapid. The present invention, by the screening to etoposide injection prescription of freeze-drying powder, finds to use specific excipient, and simultaneously using antioxygen Agent and chelating agen, the stability of the injection etoposide freeze-dried powder finished product obtaining is high, and impurity is few, Small side effects, Safety is higher, has the advantages of convenient storage and use, low production cost.Chinese patent cn 105232478 a discloses one Plant aminobutyric acid freeze-dried powder and preparation method thereof, this aminobutyric acid freeze-dried powder is adjusted by aminobutyric acid, sodium sulfite, Mannitol, ph Section agent is made, and by the addition of stabilizer, prepared aminobutyric acid freeze-dried powder end product quality is stable, drug content is high, relevant thing Matter content is low, and restrained effectively the phenomenons such as spray bottle or severe atrophy.
Prepare the less of lyophilized powder with integration of edible and medicinal herbs article for raw material: Chinese patent cn 103610141 b discloses one kind The preparation method of freeze-dried ginkgo powder, belongs to agricultural product, food processing technology field.It comprises the following steps that: after crushing Semen Ginkgo put in clear water soak, then take out and drain；Semen Ginkgo after draining carries out microwave ripening；The ginkgo shelling of ripening, go Capsule, then carries out pulverizing, extrudes in blocks and removing oil fat；Semen Ginkgo after defat carries out pre-freeze, carries out intensification lyophilizing after pre-freeze；Lyophilizing Semen Ginkgo grinds afterwards, that is, obtain freeze-dried ginkgo powder.The freeze-dried ginkgo powder harmful components that the present invention obtains are few, lightweight it is easy to Carry and transport, Semen Ginkgo more concentrates, nutritional labeling is higher.Chinese patent cn 103229920 a discloses a kind of Mel lyophilizing Powder, it includes Mel, and described Mel is a kind of powder lyophilized powder being encapsulated in airtight package container.Described powder Shape lyophilized powder can be that in powder lyophilized powder described in every 100 grams, glucose content is 5 grams to 38 grams, and fructose content is 90 grams To 40 grams, cane sugar content is less than 3 grams, and enzyme values are more than 9.The honeybee that the manufacture method of the Mel lyophilized powder being provided using the present invention is obtained Sweet lyophilized powder, improves the method for the content of former hydromel so that Mel can be at low temperature using adding appropriate cold distilled water Forming ice shelf structure, thus realizing lyophilization so that finally giving the Mel lyophilized powder of solid state, and being encapsulated in airtight In packing container, thus realizing both having preserved the original nutritional labeling of Fresh Honey, enable to preserve for a long time again.Chinese patent Cn101199344 b discloses " branch is integrated " preparation of a kind of lyophilized powder producing asparagus ' all-nutrient active matter concentrated juice Method: broken Germinatus Phragmitiss tissue is squeezed the juice, then by the protein enzyme Large molecule active material in juice, with bioflocculation Precipitant is processed, then with solid-state " green mud " (a) form isolation, is allowed to depart from Germinatus Phragmitiss enzyme water soluble substrate, and avoids dense The impact of the change sexual factor (temperature, air, microorganism) in contracting technique.Small molecular in metastable supernatant, through 10 Cryoconcentration about times is enriched to clear and bright micro- yellow concentrated juice (b).B branch is because of flocculating and settling agent and citric acid Addition and avoid browning reaction.A, b bis- branch can individually can also mix after lyophilizing, that is, be integrated into end-product lyophilized powder. Chinese patent cn 105249129 a discloses a kind of blue berry lyophilizing powder solid beverage, is related to health beverage processing technique field, Beverage consists of: blue berry full fruit jelly dry powder 15-30%, flavoring agent (white sugar etc.) white sugar 5%-15%, beverage is with the full fruit jelly of blue berry Dry powder directly brews for raw material and forms, and processing technique mainly includes lyophilizing, packaging and sterilization three process, and processing technique is simple, Equipment investment is few, low production cost.Related process technology ripe it is easy to industrialized production.At full fruit pan feeding, low-temperature freeze drying processing Reason, product is of high nutritive value, and health care is good, adapts to market trend.
Although lyophilized powder has a lot of advantages with respect to other powder, can produce big during vacuum lyophilization The ice crystal of amount, to animal and plant cellss, the organizational structure of product, quality and basic boom, nutrient substance cell etc. " cold quick property " Material all can cause to stab, and then reduces nutrient substance and bioactive substance potency, especially prepares in active microorganism powder Aspect can greatly improve the fatality rate of microorganism, significantly reduces number of viable, patent disclosed above whether field of medicaments Freeze-dried powder, or the lyophilizing powder food in integration of edible and medicinal herbs field, are all changed less than on the basis of existing vacuum lyophilization Enter to solve the problems, such as refrigerating process frost damage prevention, and although some pharmaceutical technology field apply cryoprotective agent, but mostly have , it is impossible to be applied to food technology field, meanwhile, lyophilized powder integration of edible and medicinal herbs raw-food material is single for malicious, harmful and chemical substance, dissolving Property is poor, feature is low and not comprehensive.
Chinese patent cn 103109930 b a kind of add sericin antifreeze peptide fruity probiotic acid milk slice, its raw material by with Under group by weight be grouped into: 10～14 parts of defatted milk powder, 2～5 parts of sucrose, 1～3 part of sericin peptide taken, 1～4 part of fruit material, freeze 0.1～0.6 part of dry active probiotic and 0.1～0.4 part of magnesium stearate, this invention additionally provides the preparation method of product, main Technological process is wanted to include: the preparation of skimmed milk food stage culture medium, the fermentation of probiotic bacteria, the interpolation of sericin antifreeze peptide, fermentation milk Vacuum lyophilization, the technique such as pressed powder, with the addition of sericin antifreeze peptide in probiotic acid milk slice and rich in b vitamin Fruit lyophilized powder, not only makes number of live bacteria of probiotics height in sour milk tablet, the bacterium vital preservation time is longer, and improves its mouthfeel wind Taste.Qi Yongming carries out short summary, and the problem that zydis technology is existed to the development of lyophilizing chip technology in recent years and clinical advantage It is analyzed, freeze-drying tablet latest Progress is introduced simultaneously, paper freeze drying technology is in international and domestic medicine The history in field, present situation and development trend, the defect that freeze drying technology exists and the technical issues that need to address (Qi Yongming. lyophilizing Tablet preparation New research progress .journal of china prescription drug 2012.10 (4) .57-59).But, with Mulberry cocoon prepares sericin antifreeze peptide cost of material height, and cryoprotective effects are single and still not highly desirable.
Prepare using integration of edible and medicinal herbs food that a kind of nutrient substance and bioactive substance content are high, function is strong, cryoprotective effects Expectation and the responsibility good, dissolubility is good, low cost instant integration of edible and medicinal herbs food lyophilized powder is those skilled in the art.
Content of the invention
The purpose of the present invention is the defect overcoming existing integration of edible and medicinal herbs food lyophilized powder, with integration of edible and medicinal herbs food as raw material, Whole process adopts extract at low temperature technique, and the extract obtaining remains the natural colored of integration of edible and medicinal herbs food, local flavor to greatest extent And mouthfeel, so that bioactive substance that extract contains and nutraceutical agents content is maximized simultaneously, raw material extraction ratio and Utilization rate highest, reduces extraction cost；Extract is in vacuum lyophilization process using prepared by natural plant raw material having The antifreeze of notable cryoprotective effects replaces traditional, existing cryoprotective agent, greatly reduces lyophilized technique further and causes Freezing loss, effectively increase biological activity and the nutritive value of extract freeze-drying powder, and with functional dietary fiber powder, benefit The raw factor and Lactobacillus plantarum powder science compound, and significantly enhance medicine food nutritional balance and the function of extract freeze-drying powder Property, dissolubility and probiotic, extend the shelf-life of product, a kind of nutrient substance and bioactive substance content be finally obtained High, function is strong, cryoprotective effects and dissolubility is good, low cost instant integration of edible and medicinal herbs food lyophilized powder.
In order to achieve the above object, the present invention employs the following technical solutions:
A kind of instant integration of edible and medicinal herbs food extracts lyophilized powder, is mainly prepared by the raw material of following parts by weight:
Integration of edible and medicinal herbs food 30-50 part, food fibre powder 10-20 part, antifreeze 5-15 part, probiotics factor 5-15 part, plant Thing lactobacilluss powder 2-10 part；
Preferably, described instant integration of edible and medicinal herbs food extracts lyophilized powder, is mainly prepared by the raw material of following parts by weight:
Integration of edible and medicinal herbs food 35-45 part, food fibre powder 13-17 part, antifreeze 8-12 part, probiotics factor 8-12 part, plant Thing lactobacilluss powder 4-8 part；
It is highly preferred that described instant integration of edible and medicinal herbs food extracts lyophilized powder, mainly by the raw material system of following parts by weight Standby:
40 parts of integration of edible and medicinal herbs food, 15 parts of food fibre powder, 10 parts of antifreeze, 10 parts of probiotics factor, Lactobacillus plantarum powder 6 Part；
Further, described integration of edible and medicinal herbs food be Fructus Crataegi, Fructus Lycii, Fructus Jujubae, Fructus Hippophae, Fructus Momordicae, Flos Lonicerae, Poria, hundred Conjunction, Semen Nelumbinis, any one or a few in Radix Puerariae are mixed with arbitrary proportion.
Further, described food fibre powder is that dietary fiber is processed and obtained through physics, chemical or biological method Soluble fiber cellulose content high, biological activity is strong, have important, positive role cellulose to human body beneficial flora, and general Logical dietary fiber is compared, and its feature, biological activity are more powerful；
Preferably, described food fibre powder is by one of inulin, apple fiber, Semen Tritici aestivi fiber, Herba avenae fatuae fiber or many Plant through obtained from micronizing, extruding puffing and enzyme enzymolysis；
It is highly preferred that the preparation method of described food fibre powder, comprise the following steps: will be fine to inulin, apple fiber, Semen Tritici aestivi Dimension 4-6:2-4:1-3 in mass ratio uniformly mixes, micronizing to 3-6 μm of particle diameter, and adjustment ground product moisture is 19- 21%, in screw speed 160-180r/min, temperature 185-200 DEG C condition extruding puffing, add expanded amount of substance 2-4 times Water, room temperature 200-400w, 35-40khz condition supersound extraction 10-15min, then in electric field intensity 20-40kv/cm, during pulse Between 400-600 μ s, carry out high voltage pulse electric field processing 10-15min under the conditions of pulse frequency 200-400hz；Adjust ph with lactic acid It is worth for 4.5-6.5, adds the enzyme of mixture quality 0.1-0.3%, digest 20-48min in 45-55 DEG C；Enzymolysis solution reduces pressure Concentrate, lyophilization obtains final product food fibre powder；
Described enzyme is cellulase, xylanase, laccase, pectase, tannase 2-4:1-3:1-2 in mass ratio: 0.5-1.5:0.2-1 uniformly mixes.
Further, described antifreeze is the plant to prepare for raw material containing the natural plants seed enriching freeze proof substrate Extract and trehalose, sericin peptide taken compound, fully dissolving forms, and both can prevent fragrance of integration of edible and medicinal herbs raw-food material etc. from volatilizing Property effective ingredient is taken out of by vapor, can reduce lyophilized powder nutrient substance again and the ice crystal of bioactive substance stabs loss, Improve the recovery rate of lyophilized powder, increased dissolubility and the stability of lyophilized powder simultaneously again, create unexpected beneficial Effect, is particularly suitable as the freeze drying protectant of integration of edible and medicinal herbs raw-food material extract freeze-drying powder preparation process；
Preferably, the quality of described antifreeze consists of: plant extract: trehalose: sericin peptide taken=37-53:2-8:1- 4；
Preferably, described plant extract is with one of winter rye, Bulbus Allii Cepae, Sabina vulgaris, the seed of Caulis et Folium Ammopiptanthi Mongolici or several Planting is raw material, again through compound enzyme segmentation enzyme after assisting bigcatkin willow acid soak, refrigerating to process through high-pressure electrostatic, high-pressure pulse electric Solve and be obtained；
It is highly preferred that the preparation method of described plant extract, comprise the steps: by winter rye, Bulbus Allii Cepae, Sabina vulgaris, The seed of Caulis et Folium Ammopiptanthi Mongolici 13-17:5-11:3-5:1-3 in mass ratio uniformly mixes, sabot, high in electric field intensity 5-7kv/cm first Pressure Electrostatic Treatment 2-4min；Then soaking at room temperature 2-4h in concentration is for the salicylic acid solution of 40-60mg/l, simultaneously in electric-field strength Degree 10-20kv/cm, burst length 150-250 μ s, carry out high voltage pulse electric field processing under the conditions of pulse frequency 150-250hz；Drift Wash, drain, stand 18-24h in 3-5 DEG C, then successively in 1-3 DEG C of cold preservation 2-4d, -3--5 DEG C of freezing 1-3d, -15--18 DEG C cold Freeze 20-30h, be immediately placed at outdoor in intensity of illumination 0.5-5 ten thousand lx natural lighting 2-4h, so that compound half is carried out immediately after thawing Pulverize, ground product particle diameter 0.5-1.5mm, be subsequently added into the water of ground product quality 4-6 times, adjusting ph value with lactic acid is 3.5-5.5； It is eventually adding the compound enzyme of mixed liquor quality 1-2%, digests 10-30min in 35-50 DEG C first, then digest in 50-60 DEG C 20-40min；Enzymolysis solution filters, filtrate reduced in volume obtains final product plant extract to solid content for 15-25%；
Described compound enzyme is cellulase, protease, amylase, pectase, Digestive Enzyme 5-7:2-4:1-3 in mass ratio: 1-2:1-2 uniformly mixes.
Further, described probiotics factor weight consists of: oligomeric isomaltose 15-25 part, oligomeric galactose 15-25 Part, oligofructose 10-20 part, oligomeric xylose 10-20 part, stachyose 5-11 part, soybean oligo saccharide 5-11 part, Raffinose 4-10 Part, Oligomeric maltose 3-7 part.
Further, described Lactobacillus plantarum powder is with Lactobacillus plantarum cgmcc no.11763 for starting strain routinely Method preparation, its viable bacteria content is: 7 × 1012-9×1012cfu/g；
Preferably, the cryoprotective agent that during described Lactobacillus plantarum powder preparation, freeze drying process adopts is prepared for the present invention Antifreeze, be remarkably improved Lactobacillus plantarum powder in freezing dry process Viable detection to more than 94%.
It is highly preferred that the mass ratio that Lactobacillus plantarum cgmcc no.11763 wet thallus are mixed with antifreeze is 1:0.6- 1.4.
The present invention another object is that the preparation method providing above-mentioned instant integration of edible and medicinal herbs food extracts lyophilized powder, including such as Lower step:
1) preparation of extracting solution: according to formula, weigh each component raw material, put into integration of edible and medicinal herbs food equipped with 0.8- first Clean 3-5min in 200-400w, 20-40khz in the ultrasonic washing unit of 1.2% sodium bicarbonate solution, drain, pretreatment, put In power 3-5kw, thickness of feed layer 2-4cm, 50-70 DEG C in microwave drying, 4-6min is dried, being then immersed in mass percent is 10-15min in the sericin peptide solution of 8-12%, takes out, and is pulverized immediately, freezing after -18--22 DEG C of freezing 30-50min Thickness of feed layer 3-5cm, ground product particle diameter 0.3-0.5mm, it is subsequently added into the water of ground product quality 4-6 times, adjust ph value with lactic acid For 3.5-5.5, at room temperature in electric field intensity 25-35kv/cm, burst length 300-500 μ s, pulse frequency 200-300hz bar Carry out high voltage pulse electric field processing 20-30min under part；Then carry out microwave exposure under the conditions of power 150-300w in room temperature to carry Take 15-20min, simultaneously in power 200-300w, under the conditions of frequency 30-40khz, carry out ultrasonic assistant extraction；Last vacuum takes off It is so incensed that fruit and vegetable juice, adds the mixed enzyme of its quality 0.2-0.6%, digest 30-50min in 40-50 DEG C, take enzymolysis solution quality 0.5- 1% Ovum Gallus domesticus album, puts in blender and stirs 10-12min in rotating speed 800-1000r/min, until Ovum Gallus domesticus album all becomes foam and obtains To egg white foams, egg white foams are added in enzymolysis solution, normal pressure boils 1-3min, is rapidly decreased to room temperature, 80-100 eye mesh screen mistake Filter, filtrate reduced in volume to solid content to 30-50% obtains integration of edible and medicinal herbs food extracting solution；
Described mixed enzyme is that cellulase, protease, pectase, Digestive Enzyme 2-4:1-3:1-2:1-2 in mass ratio is uniform Mixing；
2) batch mixing: the 4-6% of antifreeze and food fibre powder quality is sequentially added in extracting solution, uniformly mixes, in work( Rate 300-500w, carries out ultrasonic dissolution 10-15min under the conditions of frequency 35-45khz, obtains mixed liquor；
3) lyophilizing: extremely -40～-50 DEG C of quick-freezing in dry storehouse put by mixed liquor, and pre-freeze 2～3h at -40～-50 DEG C；So To be evacuated in dry storehouse after 15～20pa afterwards, progressively heat up in 8-10h and control mixed liquor final temperature -15～- 18℃；Then, in the case that in dry storehouse, vacuum is constant, adjusting temperature in dry storehouse makes it progressively be warming up in 5-7h Room temperature, to lyophilized powder moisture be less than 4% when discharging, with Lactobacillus plantarum powder, remaining food fibre powder, prebiotic under aseptic condition The factor uniformly mixes to obtain instant integration of edible and medicinal herbs food extracts lyophilized powder.
Lactobacillus plantarum (lactobacillus plantarum) xh of the present invention is preserved in China on November 30th, 2015 Microbiological Culture Collection administration committee common micro-organisms center (abbreviation cgmcc), preserving number is cgmcc no.11763, preservation Address is: city of BeiJing, China Chaoyang District North Star West Road 1 institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.
Lactobacillus plantarum probiotic properties are as follows:
Lactobacillus plantarum cgmcc no.11763 provided by the present invention finds, through experiment, the condition that can be 1.50 in ph Lower survival, still in existing state after 1% cholate is cultivated 4 hours；Lactobacillus plantarum cgmcc no.11763 degraded nitrous acid Salt speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and, when producing Pickles, whole sweat nitrite is dense for this strain Degree is in below 4.8mg/kg；Cgmcc no.11763, after fermentation 60h hour, can reach 64.76% to degrading rate of cholesterol. Cgmcc no.11763 Adhering capacity measure from coagulation rate be 95.71%.
Lactobacillus plantarum cgmcc no.11763 is to cholesterol degradation capability study and mensure:
1ml cgmcc no.11763 mother solution is taken to be inoculated in the mrs cholesterol fluid medium (cholesterol level of 10ml 0.1mg/ml, ph 6.2) in, 37 DEG C of constant temperature standing cultivates the mrs that 20h, 40h, 60h are standby, to access 1ml sterilized water respectively Cholesterol culture medium is comparison, takes at bacteria liquid sample and each 1ml, 9000r/min, 4 DEG C of the comparison liquid of above culture different time Centrifugation 10min, obtains fermented supernatant fluid, in o-phthalaldehyde method mensure supernatant, cholesterol level is (particularly as follows: take each supernatant 0.1ml in corresponding test tube, acetic acid 0.3ml on the rocks, o-phthalaldehyde(OPA) 0.15ml of 1mg/ml, be slowly added into concentrated sulphuric acid 1.0ml, mix homogeneously.Room temperature stands 10min, surveys light absorption value under 550nm).Each 3 repetitions of process, in kind make Make cholesterol standard curve, calculate cholesterol level and degradation rate in supernatant, the results are shown in Table 1.Understand, cgmccno.11763 There is good Degradation to cholesterol, after fermentation 60h hour, degradation rate can reach 64.76%.
The degraded situation to cholesterol for the table 1.
Degradation time (h) 0 20h 40h 60h
Cholesterol level (mg/ml) 0.2273±0.0058 0.1356±0.0018 0.1011±0.0094 0.801±0.0231
Degrading rate of cholesterol % 40.34% 55.52% 64.76%
The bile tolerance test of Lactobacillus plantarum cgmcc no.11763 bacterial strain:
Take cgmccno.11763 bacterium solution 1ml inoculation strain in containing different cholate (concentration gradients be 0.0%, 0.2%, 0.4%th, 0.6%, 0.8%, 1%) 10ml mrs fluid medium (ph=6.4), be placed at 37 DEG C and cultivate 0 respectively, 2,4h, Each processes 3 repetitions.Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilution factor solution, take 0.1ml to dilute Liquid is coated with mrs, be inverted in 37 DEG C of biochemical cultivation cases culture 48 hours (each dilution factor do 3 parallel) record calculate flat Bacterium number number on plate.The results are shown in Table 2.Understand that this bacterium increment of bacterium after gallbladder salinity is 1% process 4h still reaches 0.59±0.92×107(cfu/ml), there is good bile tolerance ability.
Table 2 bile tolerance ability detects [(± s) × 107cfu/ml]
The acid resistance test of Lactobacillus plantarum cgmcc no.11763 bacterial strain
Hlx37 mother solution is taken to inoculate strain in different ph values (ph gradient is 1.5,2.0,2.5,3.0,3.5,4.0) by 1ml 10mlmrs fluid medium, be placed at 37 DEG C and cultivate 0 respectively, 2,4h, each 3 repetitions of process.Respectively take 1ml sample bacterium solution in Mix in 9ml normal saline, prepare dilute solution, take 0.1ml diluent to be coated with mrs, fall in 37 DEG C of biochemical cultivation cases Put the bacterium colony number on culture 48 hours (each dilution factor do 3 parallel) record flat board.The results are shown in Table 3.Illustrate that this bacterium has Very strong acid-fast ability.
Table 3 acid-fast ability detects [(± s) × 107cfu/ml]
The Adhering capacity of Lactobacillus plantarum cgmcc no.11763 measures
Culture cgmcc no.11763 (mrs fluid medium), escherichia coli dh5 α (lb fluid medium) 24h must ferment Liquid, is respectively placed in 3000r/min, is centrifuged 10min at 4 DEG C, collect bacterium mud, use the sterile phosphate buffer of ph=7.0 respectively (pbs) washing bacterium mud 2 times (adds pbs in bacterium colony, after concussion mix homogeneously, is placed in 3000r/min, is centrifuged at 4 DEG C 10min, collects thalline).From coagulation rate (%): with aseptic pbs, bacterium mud cgmcc no.11763 is formed at wavelength 600nm Light absorption value be the suspension bacteria liquid of 0.4 ± 0.1 (a0) and bacteria suspension, measure light absorption value a24, from coagulation rate (%) after standing 24h Formula is (a0 a24)/a0.；His coagulation rate (%): the outstanding bacterium solution of cgmcc no.11763 and escherichia coli dh5 α is adjusted to Light absorption value at wavelength 600nm is the mix suspending bacterium solution of 0.6 ± 0.1 (a0).Light absorption value a 24 is measured after standing 24h, he Coagulation rate (%) formula is (a 0 a 24)/a 0.Measurement result is shown in Table 5 it is known that cgmcc no.11763's from coagulation rate is 95.71%, there is very strong Adhering capacity.
Table 4 Adhering capacity table
The bacterial strain physiological property of Lactobacillus plantarum cgmcc no.11763
It is micro- that described Lactobacillus plantarum (lactobacillus plantarum) xh was preserved in China on November 30th, 2015 Biological inoculum preservation administration committee common micro-organisms center (abbreviation cgmcc), preserving number is cgmcc no.11763, preservation ground Location is: city of BeiJing, China Chaoyang District North Star West Road 1 institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.
This bacterial strain feature is as follows: examines under a microscope, this bacterial strain is rod-short, Gram’s staining is positive, atrichia, Do not produce spore；On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circle, and edge is more neat.
Physicochemical characteristicses are: catalase (-), gelatin liquefaction (-), indole experiment (+), mobility (-), fermentation gas (-), nitrate reductase (-), fermentation gas (-), product hydrogen sulfide gas (-), grow in ph4.0mrs culture medium (+).Through Physiology and biochemistry is accredited as Lactobacillus plantarum (lactobacillus plantarum), is named as Lactobacillus plantarum (lactobacillus plantarum)xh.
Bacterial strain can at 57 DEG C well-grown, glucose tolerance be 275g/l.
Lactobacillus plantarum of the present invention, by gathering people Li Jianshu, separates in Yoghourt from Xinjiang Uygur fellow-villager family and obtains, Acquisition time on June 2nd, 2015.
Lactobacillus plantarum cgmcc no.11763 of the present invention can be survived through experiment discovery under conditions of ph is for 1.50, Still in existing state after 1% cholate culture 4 hours；Lactobacillus plantarum cgmcc no.11763 degrading nitrite speed is fast, Capacity of decomposition reaches 10.9mg/h/kg, and, when producing Pickles, whole sweat nitrite concentration is in 4.8mg/ for this strain Below kg；Cgmccno.11763, after fermentation 60h hour, can reach 64.76% to degrading rate of cholesterol.cgmcc No.11763 Adhering capacity measure from coagulation rate be 95.71%, bacterial strain can at 57 DEG C well-grown, glucose-tolerant energy Power is 275g/l.
Beneficial effect:
The present invention with integration of edible and medicinal herbs food as raw material, initially be cleaned by ultrasonic to integration of edible and medicinal herbs food integration of edible and medicinal herbs food Carry out parasite killing go out ovum, killing microorganisms, remove pesticide residues and heavy metal ion etc., substantially increase the food safety of lyophilized powder Property；Using microwave drying make integration of edible and medicinal herbs food in short-term, low-temperature explosion-puffing drying while be mainly enzyme denaturing, fixation, maximum Remain to limit the natural colored of integration of edible and medicinal herbs food, the integration of edible and medicinal herbs natural food color and luster after microwave color fixing is follow-up Highly stable in the course of processing, will not variable color, colour fading, color and luster is very bright-coloured, can keep the natural colored of integration of edible and medicinal herbs food；? Soak rehydration in aqueous solution containing sericin peptide taken, integration of edible and medicinal herbs food active matter that is chilled and causing can be reduced to greatest extent Matter and the loss of cold quick property composition, improve the extraction ratio of integration of edible and medicinal herbs food effective ingredient, and whole process adopts extract at low temperature technique, To be cleaned by ultrasonic, microwave deactivating enzyme fixation and expanded, high-pressure pulse electric extraction, ultrasonic wave added microwave extraction, biological enzymolysis and true Empty degassing organically combines, and the extract obtaining remains natural colored, local flavor and the mouthfeel of integration of edible and medicinal herbs food to greatest extent, So that bioactive substance that extract contains and nutraceutical agents content is maximized, raw material extraction ratio and utilization rate are the most simultaneously Height, reduces extraction cost；The egg white foams prepared using Ovum Gallus domesticus album, to enzymolysis solution flocks in short-term, further increase lyophilized powder Dissolubility and stability；Part food fibre powder is added in extracting solution and uniformly mixes with antifreeze, the solid of batch mixing can be improved Thing content, reduces moisture, alleviates the burden of freezing process, shorten freeze-drying time, improve lyophilizing efficiency, through ultrasonic Dissolving, further enhancing homogeneity, stability and the dissolubility of batch mixing, and then improves the homogeneity of lyophilized powder, stability And dissolubility；Extract has the freeze proof of notable cryoprotective effects in vacuum lyophilization process using prepared by natural plant raw material Agent replaces traditional, existing cryoprotective agent, greatly reduces the freezing loss that lyophilized technique causes further, effectively improves The biological activity of extract freeze-drying powder and nutritive value, and multiple with functional dietary fiber powder and Lactobacillus plantarum powder science Join, significantly enhance medicine food nutritional balance and feature, the dissolubility and probiotic of extract freeze-drying powder, extend product Shelf-life, be finally obtained that a kind of nutrient substance and bioactive substance content are high, function is strong, cryoprotective effects and dissolubility good, The instant integration of edible and medicinal herbs food lyophilized powder of low cost.Concrete test effect is shown in embodiment 7-13, and particular technique principle is as follows:
1. the Lactobacillus plantarum powder Viable detection of the present invention is high, function is complete, probiotic strong, Lactobacillus plantarum cgmcc No.11763 can be survived through experiment discovery under conditions of ph is for 1.50, still in survival shape after 1% cholate is cultivated 4 hours State；Lactobacillus plantarum cgmcc no.11763 degrading nitrite speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, this strain When producing Pickles, whole sweat nitrite concentration is in below 4.8mg/kg；Cgmcc no.11763 is in fermentation 60h After hour, 64.76% be can reach to degrading rate of cholesterol.Cgmcc no.11763 Adhering capacity measure from coagulation rate be 95.71%.
2. the antifreeze of present invention preparation is the plant to prepare for raw material containing the natural plants seed enriching freeze proof substrate Thing extract compounds and forms with trehalose, sericin peptide taken, and without any chemical substance and food additive, natural freeze proof substrate is complete Face, abundant, antifreeze proteins and antifreeze peptide content high, substantially increase the foodsafety of lyophilized powder, both can prevent medicine food with The volatile effective components such as the fragrance of source raw-food material are taken out of by vapor, can reduce lyophilized powder nutrient substance and biological activity again The ice crystal of material stabs loss, improves the recovery rate of lyophilized powder, increased dissolubility and the stability of lyophilized powder simultaneously again, Create unexpected beneficial effect, be particularly suitable as the jelly of integration of edible and medicinal herbs raw-food material extract freeze-drying powder preparation process Dry protective agent, can substitute existing cryoprotective agent completely, and produce more preferable effect；Meanwhile, it is applied to plant breast The freeze-drying process of bacillus powder, is remarkably improved Viable detection to more than 94%.Plant extract therein will be containing abundant anti- The natural plants seed feed science freezing substrate compounds, and high-pressure electrostatic is processed, high-pressure pulse electric assists Induced by Salicylic Acid, low Warm segmentation Stress treatment and natural lighting organically combine so that itself containing the side of body in outer boundary environment for the active seed of freeze proof substrate Force to make peace under induction, freeze proof matrix components have obtained the most comprehensive, synthesis the abundantest and accumulation, and after biological enzymolysis, maximizing is molten Go out, freeze proof peptide content can be made simultaneously to increase, improve dissolubility and the stability of plant extract, and then eliminate because of antifreeze The problem of the lyophilized powder dissolubility difference causing, is compounded with food fibre powder, Lactobacillus plantarum powder, probiotics factor science, effect is more Good.
3. micronizing, extruding puffing, supersound extraction, high-pressure pulse electric are extracted by the food fibre powder of present invention preparation With biological enzymolysis combination of sciences, gained food fibre powder retentiveness, dilatancy, thickening property are higher, than changing of single method preparation Property dietary fiber improve 20-40%, and do not affected by acid, alkali, salt, soluble fiber cellulose content is high, than single method preparation Modified dietary fiber improve 10-30% it is easier to be utilized by lactic acid bacteria, improve lactic acid bacteria in the growth of human body intestinal canal and breeding Ability, increases the type and quantity of probiotic bacteria flora, reduces human body intestinal canal ph value, improves human body intestinal canal microecological environment；Absorption Ability is strong, and after modified, the specific surface area of cellulose increases, and network is enriched, and absorption affinity strengthens, chelating, absorption cholesterol , suppression human body absorption to they higher with the organic molecule ability of bile acids；Ion-exchange capacity strengthens, to metal unit Element, particularly heavy metal element adsorption effect is higher, effectively prevent human body heavy metal poisoning；Adjust and maintain intestinal microbial population Resident time, strengthens digestion and the absorbability of intestinal, improves immunity of organisms；Effectively facilitate gastrointestinal peristalsiss, slow down and eliminate The untoward reaction such as flatulence, abdominal distention；Powerful embedding effect can prevent environment (oxygen, temperature, illumination, water activity etc.) factor pair The impact of lyophilized powder quality, stabilizes the biological activity of lyophilized powder further, extends the shelf-life of lyophilized powder.
4. the preparation method of lyophilized powder of the present invention adopts non-thermal technology technology, high working efficiency, and energy resource consumption is few, to environment Close friend, pollution-free, decrease food additive and the introducing of chemical assistant, effectively increase foodsafety, improve product Quality and reduce production cost it is achieved that low-carbon (LC) productive target, can scale, industrialized development.Particularly: 1) adopt microwave Be dried make integration of edible and medicinal herbs food in short-term, low-temperature explosion-puffing drying while be mainly enzyme denaturing, fixation, remain to greatest extent The natural colored of integration of edible and medicinal herbs food, the integration of edible and medicinal herbs natural food color and luster after microwave color fixing is non-in the follow-up course of processing Often stable, will not variable color, colour fading, color and luster is very bright-coloured, can keep the natural colored of integration of edible and medicinal herbs food；2) containing sericin peptide taken Aqueous solution in soak rehydration, chilled and the integration of edible and medicinal herbs food active substance that causes and cold quick property can be reduced to greatest extent The loss of composition, improves the extraction ratio of integration of edible and medicinal herbs food effective ingredient, and whole process adopts extract at low temperature technique, will be ultrasonic clear Wash, microwave deactivating enzyme fixation and expanded, high-pressure pulse electric extraction, ultrasonic wave added microwave extraction, biological enzymolysis and vacuum outgass have Machine combines, and the extract obtaining remains natural colored, local flavor and the mouthfeel of integration of edible and medicinal herbs food to greatest extent, simultaneously so that Bioactive substance and the maximization of nutraceutical agents content, raw material extraction ratio and utilization rate highest that extract contains, reduce Extraction cost；3) egg white foams adopting Ovum Gallus domesticus album preparation, to enzymolysis solution flocks in short-term, further increase the dissolving of lyophilized powder Property and stability；4) by extracting solution appropriateness concentrating under reduced pressure, it is subsequently adding part food fibre powder and antifreeze uniformly mixes, can carry The solid content of high batch mixing, reduces moisture, alleviates the burden of freezing process, shorten freeze-drying time, improve jelly Dry efficiency, through ultrasonic dissolution, further enhancing homogeneity, stability and the dissolubility of batch mixing, and then improves lyophilized powder Homogeneity, stability and dissolubility, improve the lyophilizing rate of freeze-drying process.
It should be noted that the eating effect of this lyophilized powder is the result that each group split-phase is mutually worked in coordination with, interacted, not letter The superposition of single raw material function, the science of each raw material components compounds and extracts, and the effect of generation is considerably beyond each single component work( Can have preferably advanced and practicality with the superposition of effect.
Specific embodiment
Describe the present invention below by specific embodiment.Unless stated otherwise, technological means used in the present invention It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, and the unrestricted present invention Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this On the premise of invention spirit and scope, the material component in these embodiments and consumption are carried out various changes or are changed Belong to protection scope of the present invention.
Prepared by embodiment 1 raw material
1. the preparation of food fibre powder
The preparation method of described food fibre powder, comprises the following steps: by inulin, apple fiber, Semen Tritici aestivi fiber by quality More uniform mixing than 5:3:2, micronizing to 5 μm of particle diameter, adjustment ground product moisture is 20%, in screw speed 170r/ Min, 192 DEG C of condition extruding puffings of temperature, the water of 3 times of the expanded amount of substance of addition, room temperature 300w, 40khz condition supersound extraction 12min, then in electric field intensity 30kv/cm, burst length 500 μ s, carry out high-pressure pulse electric under the conditions of pulse frequency 300hz Process 12min；Adjusting ph value with lactic acid is 5.5, adds the enzyme of mixture quality 0.2%, digests 35min in 50 DEG C；Enzyme Solution liquid concentrating under reduced pressure, lyophilization obtain final product food fibre powder；
Described enzyme is cellulase, xylanase, laccase, pectase, tannase 3:2:1.5:1:0.6 in mass ratio Uniformly mix.
2. the preparation of plant extract
The preparation method of described plant extract, comprise the steps: by winter rye, Bulbus Allii Cepae, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici kind Son 15:8:4:2 in mass ratio uniformly mixes, sabot, processes 3min in electric field intensity 6kv/cm high-pressure electrostatic first；Then dense Spend for soaking at room temperature 3h in the salicylic acid solution of 50mg/l, simultaneously in electric field intensity 15kv/cm, burst length 200 μ s, pulse frequency Carry out high voltage pulse electric field processing under the conditions of rate 200hz；Rinse, drain, stand 21h in 4 DEG C, then successively in 2 DEG C of cold preservations 3d, -4 DEG C of freezing 2d, -16 DEG C of freezing 25h, it is immediately placed at outdoor in intensity of illumination 30,000 lx natural lighting 3h, so that compound half is solved Pulverized immediately after jelly, ground product particle diameter 1.0mm, be subsequently added into the water of 5 times of ground product quality, adjusting ph value with lactic acid is 4.5；It is eventually adding the compound enzyme of mixed liquor quality 1.5%, digests 20min in 42 DEG C first, then digest in 55 DEG C 30min；Enzymolysis solution filters, filtrate reduced in volume to solid content obtains final product plant extract for 20%；
Described compound enzyme is cellulase, protease, amylase, pectase, Digestive Enzyme 6:3:2:1.5:1.5 in mass ratio Uniformly mix.
The food fibre powder that example 2 below -6 is used, plant extract are embodiment 1 and prepare.
Embodiment 2
A kind of wolfberry fruit extract lyophilized powder, is mainly prepared by the raw material of following parts by weight:
40 parts of Fructus Lycii, 15 parts of food fibre powder, 10 parts of antifreeze, 10 parts of probiotics factor, 6 parts of Lactobacillus plantarum powder；
The quality of described antifreeze consists of: plant extract: trehalose: sericin peptide taken=45:5:2；
Described probiotics factor weight consists of: 20 parts of oligomeric isomaltose, 20 parts of oligomeric galactose, 15 parts of oligofructose, 15 parts of oligomeric xylose, 8 parts of stachyose, 8 parts of soybean oligo saccharide, 7 parts of Raffinose, 5 parts of Oligomeric maltose；
Described Lactobacillus plantarum powder is to be prepared according to a conventional method with Lactobacillus plantarum cgmcc no.11763 for starting strain , its viable bacteria content is: 9 × 1012cfu/g；Wherein, freeze drying process adopt cryoprotective agent for the present invention prepare upper State antifreeze, the mass ratio that wet thallus are mixed with antifreeze is 1:1.
Preparation method, comprises the steps:
1) preparation of extracting solution: according to formula, weigh each component raw material, put into Fructus Lycii equipped with 1% sodium bicarbonate first Clean 4min in 300w, 30khz in the ultrasonic washing unit of solution, drain, put in microwave drying in power 4kw, thickness of feed layer 3cm, 60 DEG C, 5min is dried, be then immersed in 12min in the sericin peptide solution that mass percent is 10%, take out, in -20 DEG C Pulverized immediately after freezing 40min, freeze thickness of feed layer 4cm, ground product particle diameter 0.4mm, be subsequently added into 5 times of ground product quality Water, with lactic acid adjust ph value be 4.5, at room temperature in electric field intensity 30kv/cm, burst length 400 μ s, pulse frequency Carry out high voltage pulse electric field processing 25min under the conditions of 250hz；Then carry out microwave exposure under the conditions of power 200w in room temperature to carry Take 18min, simultaneously in power 250w, under the conditions of frequency 35khz, carry out ultrasonic assistant extraction；Last vacuum outgass obtain fruit and vegerable Juice, adds the mixed enzyme of its quality 0.4%, digests 40min in 45 DEG C, takes the Ovum Gallus domesticus album of enzymolysis solution quality 0.8%, put into blender In stir 11min in rotating speed 900r/min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, egg white foams are added enzymolysis In liquid, normal pressure boils 2min, is rapidly decreased to room temperature, 90 mesh sieve net filtrations, filtrate reduced in volume to solid content to 40% Fructus lycii extracted solution；
Described mixed enzyme uniformly mixes for cellulase, protease, pectase, Digestive Enzyme 3:2:1.5:1.5 in mass ratio；
2) batch mixing: the 5% of antifreeze and food fibre powder quality is sequentially added in extracting solution, uniformly mixes, in power 400w, carries out ultrasonic dissolution 12min under the conditions of frequency 40khz, obtains mixed liquor；
3) lyophilizing: extremely -45 DEG C of quick-freezing in dry storehouse put by mixed liquor, and pre-freeze 2.5h at -45 DEG C；Then by dry storehouse After being evacuated to 18pa, progressively heat up and control mixed liquor final temperature in 9h at -17 DEG C；Then vacuum in dry storehouse In the case of constant, adjusting temperature in dry storehouse makes it progressively be warming up to room temperature in 6h, goes out when being less than 4% to lyophilized powder moisture Material, uniformly mixes to obtain wolfberry fruit extract lyophilizing with Lactobacillus plantarum powder, remaining food fibre powder, probiotics factor under aseptic condition Powder.
Embodiment 3
A kind of Fructus Jujubae extract lyophilized powder, is mainly prepared by the raw material of following parts by weight:
35 parts of Fructus Jujubae, 13 parts of food fibre powder, 8 parts of antifreeze, 8 parts of probiotics factor, 4 parts of Lactobacillus plantarum powder；
The quality of described antifreeze consists of: plant extract: trehalose: sericin peptide taken=37:2:1；
Described probiotics factor weight consists of: 15 parts of oligomeric isomaltose, 15 parts of oligomeric galactose, 10 parts of oligofructose, 10 parts of oligomeric xylose, 5 parts of stachyose, 5 parts of soybean oligo saccharide, 4 parts of Raffinose, 3 parts of Oligomeric maltose；
Described Lactobacillus plantarum powder is to be prepared according to a conventional method with Lactobacillus plantarum cgmcc no.11763 for starting strain , its viable bacteria content is: 8 × 1012cfu/g；Wherein, freeze drying process adopt cryoprotective agent for the present invention prepare upper State antifreeze, the mass ratio that wet thallus are mixed with antifreeze is 1:0.6.
Preparation method, comprises the steps:
1) preparation of extracting solution: according to formula, weigh each component raw material, put into Fructus Jujubae equipped with 0.8% bicarbonate first Clean 3min in 200w, 20khz in the ultrasonic washing unit of sodium solution, drain, enucleation, section, put in microwave drying in power 3kw, thickness of feed layer 2cm, 50 DEG C, 4min is dried, be then immersed in 10min in the sericin peptide solution that mass percent is 8%, take Go out, pulverized immediately after -18 DEG C of freezing 30min, freeze thickness of feed layer 3cm, ground product particle diameter 0.3mm, be subsequently added into powder The water that 4 times of the quality that minces, adjusting ph value with lactic acid is 3.5, at room temperature in electric field intensity 25kv/cm, burst length 300 μ s, Carry out high voltage pulse electric field processing 20min under the conditions of pulse frequency 200hz；Then carry out micro- under the conditions of power 150w in room temperature Amplitude, according to extracting 15min, simultaneously in power 200w, carries out ultrasonic assistant extraction under the conditions of frequency 30khz；Last vacuum outgass Obtain fruit and vegetable juice, add the mixed enzyme of its quality 0.2%, digest 30min in 40 DEG C, take the Ovum Gallus domesticus album of enzymolysis solution quality 0.5%, put into Stir 10min in rotating speed 800r/min in blender, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, by egg white foams plus Enter in enzymolysis solution, normal pressure boils 1min, be rapidly decreased to room temperature, 80 mesh sieve net filtrations, filtrate reduced in volume to solid content is extremely 30% obtains jujube extract；
Described mixed enzyme uniformly mixes for cellulase, protease, pectase, Digestive Enzyme 2:1:1:1 in mass ratio；
2) batch mixing: the 4% of antifreeze and food fibre powder quality is sequentially added in extracting solution, uniformly mixes, in power 300w, carries out ultrasonic dissolution 10min under the conditions of frequency 35khz, obtains mixed liquor；
3) lyophilizing: extremely -40 DEG C of quick-freezing in dry storehouse put by mixed liquor, and pre-freeze 2h at -40 DEG C；Then take out in dry storehouse Vacuum, to 15pa, progressively heats up and controls mixed liquor final temperature at -15 DEG C in 8h；Then in dry storehouse, vacuum is not In the case of change, adjusting temperature in dry storehouse makes it progressively be warming up to room temperature in 5h, goes out when being less than 4% to lyophilized powder moisture Material, uniformly mixes to obtain Fructus Jujubae extract lyophilizing with Lactobacillus plantarum powder, remaining food fibre powder, probiotics factor under aseptic condition Powder.
Embodiment 4
A kind of Fructus Crataegi extract lyophilized powder, is mainly prepared by the raw material of following parts by weight:
45 parts of Fructus Crataegi, 17 parts of food fibre powder, 12 parts of antifreeze, 12 parts of probiotics factor, 8 parts of Lactobacillus plantarum powder；
The quality of described antifreeze consists of: plant extract: trehalose: sericin peptide taken=53:8:4；
Described probiotics factor weight consists of: 25 parts of oligomeric isomaltose, 25 parts of oligomeric galactose, 20 parts of oligofructose, 20 parts of oligomeric xylose, 11 parts of stachyose, 11 parts of soybean oligo saccharide, 10 parts of Raffinose, 7 parts of Oligomeric maltose；
Described Lactobacillus plantarum powder is to be prepared according to a conventional method with Lactobacillus plantarum cgmcc no.11763 for starting strain , its viable bacteria content is: 7 × 1012cfu/g；Wherein, freeze drying process adopt cryoprotective agent for the present invention prepare upper State antifreeze, the mass ratio that wet thallus are mixed with antifreeze is 1:1.4.
Preparation method, comprises the steps:
1) preparation of extracting solution: according to formula, weigh each component raw material, put into Fructus Crataegi equipped with 1.2% bicarbonate first In the ultrasonic washing unit of sodium solution in 400w, 40khz clean 5min, drain, pretreatment, put in microwave drying in power 5kw, Thickness of feed layer 4cm, 70 DEG C, 6min is dried, be then immersed in 15min in the sericin peptide solution that mass percent is 12%, take out, Pulverized immediately after -22 DEG C of freezing 50min, freeze thickness of feed layer 5cm, ground product particle diameter 0.5mm, be subsequently added into ground product The water that 6 times of quality, adjusting ph value with lactic acid is 5.5, at room temperature in electric field intensity 35kv/cm, burst length 500 μ s, pulse Carry out high voltage pulse electric field processing 30min under the conditions of frequency 300hz；Then carry out microwave spoke under the conditions of power 300w in room temperature According to extracting 20min, simultaneously in power 300w, under the conditions of frequency 40khz, carry out ultrasonic assistant extraction；Last vacuum outgass obtain fruit Vegetable juice, adds the mixed enzyme of its quality 0.6%, digests 50min in 50 DEG C, takes the Ovum Gallus domesticus album of enzymolysis solution quality 1%, put into blender In stir 12min in rotating speed 1000r/min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, egg white foams are added enzyme In solution liquid, normal pressure boils 3min, is rapidly decreased to room temperature, 100 mesh sieve net filtrations, filtrate reduced in volume to solid content to 50% Obtain hawthorn extract；
Described mixed enzyme uniformly mixes for cellulase, protease, pectase, Digestive Enzyme 4:3:2:2 in mass ratio；
2) batch mixing: the 6% of antifreeze and food fibre powder quality is sequentially added in extracting solution, uniformly mixes, in power 500w, carries out ultrasonic dissolution 15min under the conditions of frequency 45khz, obtains mixed liquor；
3) lyophilizing: extremely -50 DEG C of quick-freezing in dry storehouse put by mixed liquor, and pre-freeze 3h at -50 DEG C；Then take out in dry storehouse Vacuum, to 20pa, progressively heats up and controls mixed liquor final temperature at -18 DEG C in 10h；Then vacuum in dry storehouse In the case of constant, adjusting temperature in dry storehouse makes it progressively be warming up to room temperature in 7h, goes out when being less than 4% to lyophilized powder moisture Material, uniformly mixes to obtain Fructus Crataegi extract lyophilizing with Lactobacillus plantarum powder, remaining food fibre powder, probiotics factor under aseptic condition Powder.
Embodiment 5
A kind of Flos Lonicerae extract lyophilized powder, is mainly prepared by the raw material of following parts by weight:
30 parts of Flos Lonicerae, 10 parts of food fibre powder, 5 parts of antifreeze, 5 parts of probiotics factor, 2 parts of Lactobacillus plantarum powder；
The quality of described antifreeze consists of: plant extract: trehalose: sericin peptide taken=37:8:1；
Described probiotics factor weight consists of: 15 parts of oligomeric isomaltose, 25 parts of oligomeric galactose, 10 parts of oligofructose, 20 parts of oligomeric xylose, 5 parts of stachyose, 11 parts of soybean oligo saccharide, 4 parts of Raffinose, 7 parts of Oligomeric maltose；
Described Lactobacillus plantarum powder is to be prepared according to a conventional method with Lactobacillus plantarum cgmcc no.11763 for starting strain , its viable bacteria content is: 9 × 1012cfu/g；Wherein, the cryoprotective agent that freeze drying process adopts is prepared by the present invention resisting Freeze agent, the mass ratio that wet thallus are mixed with antifreeze is 1:0.8.
Preparation method, comprises the steps:
1) preparation of extracting solution: according to formula, weigh each component raw material, put into Flos Lonicerae equipped with 0.8% carbonic acid first In the ultrasonic washing unit of hydrogen sodium solution in 400w, 20khz clean 5min, drain, section, put in microwave drying in power 3kw, Thickness of feed layer 4cm, 50 DEG C, 6min is dried, be then immersed in 15min in the sericin peptide solution that mass percent is 8%, take out, Pulverized immediately after -18 DEG C of freezing 50min, freeze thickness of feed layer 3cm, ground product particle diameter 0.5mm, be subsequently added into ground product The water that 4 times of quality, adjusting ph value with lactic acid is 5.5, at room temperature in electric field intensity 25kv/cm, burst length 500 μ s, pulse Carry out high voltage pulse electric field processing 30min under the conditions of frequency 200hz；Then carry out microwave spoke under the conditions of power 150w in room temperature According to extracting 20min, simultaneously in power 200w, under the conditions of frequency 40khz, carry out ultrasonic assistant extraction；Last vacuum outgass obtain fruit Vegetable juice, adds the mixed enzyme of its quality 0.2%, digests 30min in 50 DEG C, takes the Ovum Gallus domesticus album of enzymolysis solution quality 1%, put into blender In stir 12min in rotating speed 800r/min, until Ovum Gallus domesticus album all becomes foam and obtains egg white foams, egg white foams are added enzymolysis In liquid, normal pressure boils 1min, is rapidly decreased to room temperature, 100 mesh sieve net filtrations, filtrate reduced in volume to solid content to 30% Flos Lonicerae extractive solution；
Described mixed enzyme uniformly mixes for cellulase, protease, pectase, Digestive Enzyme 2:3:1:2 in mass ratio；
2) batch mixing: the 4% of antifreeze and food fibre powder quality is sequentially added in extracting solution, uniformly mixes, in power 300-500w, carries out ultrasonic dissolution 10min under the conditions of frequency 45khz, obtains mixed liquor；
3) lyophilizing: extremely -40 DEG C of quick-freezing in dry storehouse put by mixed liquor, and pre-freeze 2h at -50 DEG C；Then take out in dry storehouse Vacuum, to 20pa, progressively heats up and controls mixed liquor final temperature at -18 DEG C in 8h；Then in dry storehouse, vacuum is not In the case of change, adjusting temperature in dry storehouse makes it progressively be warming up to room temperature in 5h, goes out when being less than 4% to lyophilized powder moisture Material, uniformly mixes to obtain Flos Lonicerae extract jelly with Lactobacillus plantarum powder, remaining food fibre powder, probiotics factor under aseptic condition Dry powder.
Embodiment 6
A kind of Fructus Crataegi, Fructus Jujubae extract lyophilized powder, are mainly prepared by the raw material of following parts by weight:
30 parts of Fructus Crataegi, 20 parts of Fructus Jujubae, 20 parts of food fibre powder, 15 parts of antifreeze, 15 parts of probiotics factor, Lactobacillus plantarum powder 10 parts；
The quality of described antifreeze consists of: plant extract: trehalose: sericin peptide taken=53:2:4；
Described probiotics factor weight consists of: 25 parts of oligomeric isomaltose, 15 parts of oligomeric galactose, 20 parts of oligofructose, 10 parts of oligomeric xylose, 11 parts of stachyose, 5 parts of soybean oligo saccharide, 10 parts of Raffinose, 3 parts of Oligomeric maltose.
Described Lactobacillus plantarum powder is to be prepared according to a conventional method with Lactobacillus plantarum cgmcc no.11763 for starting strain , its viable bacteria content is: 9 × 1012cfu/g；Wherein, the cryoprotective agent that freeze drying process adopts is prepared by the present invention resisting Freeze agent, the mass ratio that wet thallus are mixed with antifreeze is 1:1.2.
Preparation method, comprises the steps:
1) preparation of extracting solution: according to formula, weigh each component raw material, first by Fructus Crataegi, Fructus Jujubae be respectively put into equipped with Clean 5min in 400w, 20khz in the ultrasonic washing unit of 0.8-1.2% sodium bicarbonate solution, drain, enucleation, section, uniformly Mixing, put in microwave drying in power 5kw, thickness of feed layer 2cm, 70 DEG C, 4min is dried, being then immersed in mass percent is 10min in 12% sericin peptide solution, takes out, and is pulverized immediately, freeze thickness of feed layer 5cm after -22 DEG C of freezing 30min, Ground product particle diameter 0.3mm, is subsequently added into the water of 6 times of ground product quality, and adjusting ph value with lactic acid is 3.5, at room temperature in electric field Intensity 35kv/cm, burst length 300 μ s, carry out high voltage pulse electric field processing 20min under the conditions of pulse frequency 300hz；Then in Room temperature carries out microwave irradiation and extraction 15min under the conditions of power 300w, simultaneously in power 300w, carries out under the conditions of frequency 30khz Ultrasonic assistant extracts；Last vacuum outgass obtain fruit and vegetable juice, add the mixed enzyme of its quality 0.6%, digest 50min in 40 DEG C, Take the Ovum Gallus domesticus album of enzymolysis solution quality 0.5%, put in blender and stir 10min in rotating speed 1000r/min, until Ovum Gallus domesticus album all becomes Foam obtains egg white foams, egg white foams is added in enzymolysis solution, normal pressure boils 3min, is rapidly decreased to room temperature, 80 eye mesh screen mistakes Filter, filtrate reduced in volume to solid content to 50% obtains Fructus Crataegi, jujube extract；
Described mixed enzyme uniformly mixes for cellulase, protease, pectase, Digestive Enzyme 4:1:2:1 in mass ratio；
2) batch mixing: the 5% of antifreeze and food fibre powder quality is sequentially added in extracting solution, uniformly mixes, in power 500w, carries out ultrasonic dissolution 15min under the conditions of frequency 35khz, obtains mixed liquor；
3) lyophilizing: extremely -50 DEG C of quick-freezing in dry storehouse put by mixed liquor, and pre-freeze 3h at -40 DEG C；Then take out in dry storehouse Vacuum, to 15pa, progressively heats up and controls mixed liquor final temperature at -15 DEG C in 10h；Then vacuum in dry storehouse In the case of constant, adjusting temperature in dry storehouse makes it progressively be warming up to room temperature in 7h, goes out when being less than 4% to lyophilized powder moisture Material, uniformly mixes to obtain Fructus Crataegi, Fructus Jujubae extraction with Lactobacillus plantarum powder, remaining food fibre powder, probiotics factor under aseptic condition Thing lyophilized powder.
Embodiment 7 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder dissolubility test
Lyophilized powder as a kind of powder mainly prepared by integration of edible and medicinal herbs food, with reference to the stability criterion of solid beverage, Clarity after dissolution time, dissolving is defined as the quality stability index of lyophilized powder, it not only directly reflects the molten of product Solution degree, also reflects product inherent quality and stability, is the most important quality control index of lyophilized powder.
Dissolution time: 1:5 in mass ratio adds 55-65 DEG C of warm water, is sufficiently stirred for, the time being completely dissolved；
Clarity characterizing method: clear 90-100 divides；Micro- turbid 80-89 divides；Muddy 70-79 divides；More muddy 60-69 Point；Seriously muddy 50-59 divides；Less than 49 points of precipitation (precipitation granule is bigger, quantity is more, and fraction is lower).
The wolfberry fruit extract lyophilized powder that prepare the embodiment of the present invention 2 and commercially available same type, same specification, with the date of manufacture After wolfberry fruit extract lyophilized powder dissolves in the glass, standing, observe clarity, every 5min observed and recorded result once, result It is shown in Table 1:
Table 1: lyophilized powder clarity testing result after dissolving
Result above shows: lyophilized powder of the present invention is compared with commercially available lyophilized powder, and preferably, dissolution time is short for dissolubility, dissolving Stability of solution afterwards is strong, and clarity is high, and presentation quality is preferable, with the prolongation of time of repose, commercially available lyophilized powder solution liquid phase Relatively unstable, produce precipitation, leverage presentation quality and the inherent quality of beverage, lyophilized powder of the present invention is also described simultaneously Preferably, eating effect is safe and stable for inherent quality.
It should be understood that the instant integration of edible and medicinal herbs food extracts lyophilized powder of embodiment of the present invention 3-6 preparation equally has There is above-mentioned experiment effect, between each embodiment and little with above-mentioned experiment effect diversity.
The change of T-CHOL after embodiment 8 edible instant integration of edible and medicinal herbs food extracts lyophilized powder
Select the adult 48 of T-CHOL 180mg/dl-250mg/dl, men and women half and half, is randomly divided into three groups；First group 200 milliliters of mineral waters are drunk in daily dinner；Second group of daily dinner Instant Drinks commonly commercially available wolfberry fruit extract lyophilized powder 40g, by matter Amount adds 55-65 DEG C of warm water than 1:5, is sufficiently stirred for, is completely dissolved；The Chinese holly of the 3rd group of daily dinner Instant Drinks embodiment of the present invention 2 Qi extract freeze-drying powder 80g, 1:5 in mass ratio add 55-65 DEG C of warm water, are sufficiently stirred for, are completely dissolved；Period eats same daily The food of sample, food includes meat, egg, vegetable and fruit.The previous day and the 20th, 40, the 60 days collection examination starting in experiment respectively The blood of the person of testing, measures the total cholesterol level in blood, result such as table 2:
Table 2: total cholesterol level testing result in blood
Time 0 day 20 days 40 days 60 days
First group (mg/dl) 202.3 203.8 206.2 209.3
Second group (mg/dl) 207.6 206.5 203.2 202.1
3rd group (mg/dl) 209.8 201.9 190.5 168.3
As seen from the above table after the wolfberry fruit extract lyophilized powder of the Instant Drinks embodiment of the present invention 2, the total gallbladder in adult human blood is solid There is significant change in the content of alcohol.Compared with commonly commercially available wolfberry fruit extract lyophilized powder, wolfberry fruit extract lyophilized powder pair of the present invention The content of the T-CHOL in adult human blood significantly reduces, and the content of the T-CHOL in the adult human blood of mineral water group Significantly increase, although commercially available wolfberry fruit extract lyophilized powder decreases, compared with product of the present invention, effect is not notable, by This understands, the present invention has good reduction cholesterol using the lyophilized powder with the specific bacterial strain preparation reducing cholesterol characteristic Health-care effect.
It should be understood that the instant integration of edible and medicinal herbs food extracts lyophilized powder prepared by embodiment of the present invention 3-6 is same There is above-mentioned technique effect, between each embodiment, diversity is not notable.
Embodiment 9 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder shelf-life implants living preparation of lactobacillus content is steady Qualitative test
The wolfberry fruit extract lyophilized powder taking the embodiment of the present invention 2 preparation is stored under room temperature 22-25 DEG C, ventilation condition respectively 3 months, 6 months, 12 months, 24 months, 36 months and measure Lactobacillus plantarum viable bacteria content, result such as table 3:
Table 3: shelf-life implants living preparation of lactobacillus content detection result
Result above shows: the storage stability of lyophilized powder of the present invention is fabulous, environment resistant (cold and hot temperature, humidity, oxygen, Illumination, moisture etc.) influence factor's ability is big, and shelf-life implants living preparation of lactobacillus content loss rate maximum (36 months) is 10%, higher than existing like product viable bacteria content, loss rate is low, long shelf-life, reflect the other biological of lyophilized powder simultaneously Active component has same storage stability.
It should be understood that the instant integration of edible and medicinal herbs food extracts lyophilized powder of embodiment of the present invention 3-6 preparation equally has There is above-mentioned experiment effect, between each embodiment and little with above-mentioned experiment effect diversity.
The sensory evaluation test of embodiment 10 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder
Invite the wolfberry fruit extract lyophilized powder phase syngenesis similar with commercially available two kinds that 24 personnel prepare to the embodiment of the present invention 2 The lyophilized powder producing the date is judged, and sense organ is given a mark, wherein specialty and each 12 of layman, professional is young, the middle age, Old each 4, men and women half and half, and layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half；Marking includes outward appearance (20 points), quality (25 points), local flavor (30 points), four aspects of mouthfeel (25 points), marking personnel independently carry out, are independent of each other, with It is accurate that result is judged in guarantee.Counted to judging result, equal score value takes approximation, retained integer, be specifically shown in Table 4:
Table 4: sensory evaluation statistical result
Note: notable (p ＜ 0.05) with a line internal standard difference lowercase letter indication difference, the different capitalization of mark represents difference Extremely notable (p ＜ 0.01), indicate same letter and represent that difference is not notable (p ＞ 0.05).
Result above shows, any one will be bright from outward appearance, quality, local flavor and mouthfeel for the lyophilized powder of present invention preparation Show and be better than commercially available lyophilized powder, particularly outward appearance, local flavor and mouthfeel is fabulous, remains integration of edible and medicinal herbs raw-food material to greatest extent Natural colored, taste and flavor, also are adapted for different age group, the consumer of different hierarchy of consumption eats simultaneously.
It should be understood that the instant integration of edible and medicinal herbs food extracts lyophilized powder of embodiment of the present invention 3-6 preparation equally has There is above-mentioned experiment effect, between each embodiment and little with above-mentioned experiment effect diversity.
The probiotic test of embodiment 11 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder
Wolfberry fruit extract lyophilized powder prepared by the embodiment of the present invention 2, with sterilized water be prepared into viable count be 2 × 1010The Lactobacillus plantarum solution of cfu/ml, saves backup in 4 DEG C；
1st, the 10ml Lactobacillus plantarum solution keeping of going bail for is injected in test tube 1, using ten times of stepwise dilutions to 10-8, take 1ml diluent, on flat board, the mrs agar culture medium being cooled to 45 DEG C is poured on flat board (sterilizing), shake rapidly after sterilizing Even.The test tube 2 that will be equipped with 10ml Lactobacillus plantarum solution again is placed in heating 15-25min in 80-90 DEG C of water-bath, after taking heating Lactobacillus plantarum solution carry out ten times of stepwise dilutions to 10-8, take 1ml diluent on flat board, be cooled to 45 DEG C by after sterilizing Mrs agar culture medium pour into upper and shake up rapidly in flat board (sterilizing).Finally by the flat board before heating and after heating all 35 Cultivate 24h under the conditions of DEG C, calculate the quantity before and after heating.
Result shows, Viable detection has reached more than 88%.
2nd, the resistance test of simulated gastric fluid and intestinal juice: take the hydrochloric acid 16.4ml of 100g/l to add distilled water diluting, make ph value point Not Wei 1.5,2.5 and 3.5, take 100ml dilute hydrochloric acid solution, be separately added into 1g pepsin so as to abundant dissolve, obtain simulation stomach Liquid, microporous filter membrane degerming (0.22 μm) is standby.Take potassium dihydrogen phosphate 6.8g, the 500ml that adds water makes dissolving, use 0.1mol/l hydroxide Sodium solution adjusts ph value to 6.8；Separately take trypsin 10g, the 100ml that adds water makes dissolving, after two liquid mixing, be diluted with water to 1000ml, obtains simulated intestinal fluid, and microporous filter membrane degerming (0.22 μm) is standby.The Lactobacillus plantarum solution that 1ml keeps is taken to be added to In the simulated gastric fluid of 9ml (i.e. ten times of stepwise dilutions), and fully mix on the oscillator rapidly, be subsequently placed in 30-45 DEG C of standing Culture 2-4h.Take out culture fluid respectively and count remaining viable count immediately when 1h, 2h, 3h, 4h, carry out with former viable count Relatively, result shows, Viable detection is 98%.Then it is taken at each 1ml of culture fluid digesting different time in simulated gastric fluid, point Be not inoculated in 9ml ph value in 6.8 simulated intestinal fluid, being placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h takes Sample, measures its viable count, is compared with former viable count, and result shows that Viable detection is 99%.
3rd, simulate the resistance test of cholate: make the solution of 1g/l with pancreatin, and add 0.8% Fel Sus domestica in the solution Salt, the naoh adjustment ph with 10% is 8.0, then with 0.45 μm of micro-filtrate membrane filtration and degerming.The plant breast that 0.5ml is kept Bacillus solution inoculum, in 4.5ml simulation cholate, obtains culture fluid after culture 24h, counts the viable count of remaining.Culture fluid is existed In sterile saline, ten times of stepwise dilutions are to 10-8, and poured into mrs, it is subsequently placed in 35 DEG C of quiescent culture 24h.Result shows Viable detection is 99%.
Above result of the test shows, the Lactobacillus plantarum in lyophilized powder of the present invention probiotic (thermostability, resistance to ph, resistance to gallbladder Salt) relatively strong, it is especially suitable for human intestines and stomach's environment, survival rate is big in simulated gastrointestinal environments, can be effectively improved gastrointestinal function.
It should be understood that the instant integration of edible and medicinal herbs food extracts lyophilized powder of embodiment of the present invention 3-6 preparation equally has There is above-mentioned experiment effect, between each embodiment and little with above-mentioned experiment effect diversity.
Embodiment 12 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder mouse intestinal performance test
Wolfberry fruit extract lyophilized powder prepared by the embodiment of the present invention 2, with sterilized water be prepared into viable count be 2 × 1010The Lactobacillus plantarum solution of cfu/ml, saves backup in 4 DEG C；
Choose common Kunming white mice 60, male and female half and half, 18-20g, conventional word is supported.Therefrom random choose 40, daily Morning 9:00 gavage lincomycin hydrochloride 0.2ml (20mg)/only, other as a control group, go out daily by gavage equivalent of same time Bacterium normal saline, continuous one week, the mouse model of preparation alteration of intestinal flora.Model group mouse diet declines, and death With obvious diarrhoea phenomenon, arrange soft excrement, profile normal aqueous divide more, bedding and padding humidity.By 40 alteration of intestinal flora mices, with Machine is divided into 2 groups, and one group 20 is only used as treatment group, the Lactobacillus plantarum solution 0.5ml (2 × 10 that daily gavage is kept10cfu/ Ml)/only, another 20 are only used as natural recovering group, daily gavage equivalent of same time sterile saline, continuous two weeks.Whole examination Test the phase 21 days, observe growth and the defecation situation of white mice daily, in the 8th, 21 days to lyophilized powder treatment group and natural recovering group Mice weighed, calculate each group weight average rate of increase, result such as table 5；Survey each group stool in mice coliform count within every 5 days Amount, calculates average, result such as table 6.Take stool in mice about 0.1g, add 3 beades in aseptic operating platform (with 0.1g excrement Sample adds 0.5ml diluent), dilute and inoculate maconkey agar culture medium, calculate every gram wet just in coliform count.
Table 5: mice Gain weight
Packet Average starting weight (g/ is only) Averagely end weight (g/ is only) Average rate of increase (%)
Natural recovering group 20.69±1.33 27.34±1.59 32.14a
Treatment group 20.41±1.45 34.28±1.62 67.96b
Table 6: the situation of coliform count in stool in mice
Lyophilized powder treatment group Mouse Weight average rate of increase (67.96%) is significantly higher than natural recovering group (32.14%)；Raise Feed solution rear intestinal escherichia coli quantity to be remarkably decreased, reduction by 97.07%, substantially less than natural recovering group (24.78%), show Lactobacillus plantarum in lyophilized powder of the present invention is colonized rapidly in white mice intestinal, forms dominant microflora, and effectively suppresses large intestine The growth and breeding of the pathogen such as bacillus, and resident time is long, continues, effectively improves intestinal performance.
It should be understood that the instant integration of edible and medicinal herbs food extracts lyophilized powder of embodiment of the present invention 3-6 preparation equally has There is above-mentioned experiment effect, between each embodiment and little with above-mentioned experiment effect diversity.
The impact to immunity of organisms for the embodiment 13 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder
1 experiment purpose
(mouse forced swimming) is tested by exercise tolerance, verifies the present invention instant integration of edible and medicinal herbs food extracts lyophilized powder Enhance immunity, antifatigue effect.
2 experiment materials and reagent
2.1 for reagent thing:
Commercially available instant integration of edible and medicinal herbs food extracts lyophilized powder (g1)；Commercially available instant integration of edible and medicinal herbs food extracts lyophilized powder (g2)；Instant integration of edible and medicinal herbs food extracts lyophilized powder (g3-g7) of embodiment of the present invention 2-6 preparation.
2.2 reagent:
Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing；Concentrated sulphuric acid (ar), Nanjing chemical reagent has Limit company；Normal saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..
3. laboratory animal
Icr mice, ♂, cleaning grade, body weight 18-22g, provided by Ningxia Medical University's comparative medicine center, little during experiment The free diet of Mus.
4. key instrument
Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804r type, eppendrof is public Department；Water-bath: dk-s26 type, the grand experimental facilitiess company limited of upper Nereid；Electronic scale: bs224s type, sartorius company；Second Table, thermometer
5. experiment packet
5.1 dosage packets and given the test agent give the time and at random mice are divided into 8 groups, every group 10, the 1st group to the 7th group Give the medicine of g1～g7 respectively, the 8th group is blank control group, give isopyknic distilled water, every group of often daily gavage 1 time, fill Stomach volume is 0.2ml/10g, continuously gives given the test agent 30 days.
The 1st group of 5.2 sample preparations are to the 7th group: weigh 2.25g drug sample, be assigned to 150ml with distilled water；Blank Group: distilled water 150ml.
6. experimental technique
After 6.1 swimming with a load attached to the body experiment last dose 30min, put mice in swimming trunk, the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of rat-tail root load 5% body weight, record mice swimming starts to the dead time, when swimming as mice Between.
6.2 mice serum carbamide measure last dose 30min after, temperature be 30 DEG C water in not swimming with a load attached to the body 90min, Pluck eyeball blood sampling 0.5ml (being not added with anticoagulant) after rest 60min, put 4 DEG C of refrigerator 3h, 2000r/min centrifugation after hemopexiss 15min, takes serum to send clinical laboratory of Affiliated Hospital of Ningxia Medical University to detect.
After the mensure last dose 30min of 6.3 hepatic glycogen, not swimming with a load attached to the body 90min in the water for 25 ± 1 DEG C for the temperature, Cervical dislocation puts to death mice, is cleaned with normal saline, and with after filter paper suck dry moisture, accurately weighing liver 100mg, hepatic glycogen is examined Test agent box detects mice hepatic glycogen content.
After the mensure last dose 30min of 6.4 blood lactase acid take a blood sample, then do not bear a heavy burden be 30 DEG C in temperature water went swimming Stop after 10min.Lactic acid instrument assay method: respectively before swimming, each blood sampling 20 μ l add after rest 20min after swimming, after swimming Enter in 40 μ l rupture of membranes liquid, fully vibration smudge cellses are measured with lactic acid instrument immediately.(blood lactase acid area under curve=5 × (before swimming The blood lactase acid value of 20min after blood lactase acid value+2 × swimming of 0min after+3 × swimming of blood lactase acid value)
7. observation index walking weight load, blood lactase acid, carbamide, glycogen initial value
8. statistical method experimental data is usedRepresent, compare between being organized using t inspection
9. experimental result
The impact to Mouse Weight for the 9.1 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder
Each group mice after giving g1～g9 medicine, front, in, shown in post-weight see table respectively, each group mice just Initial body weight and weightening body weight compare equal no difference of science of statistics (p ＞ 0.05) with matched group, show that g1～g9 medicine is all no obvious Toxicity.Experimental result refers to table 7.
The original body mass of table 7 swimming with a load attached to the body experiment mice, mid-term body weight and end body weight
The impact to the mice burden swimming time for the 9.2 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder
After orally administration mice g1～g7 medicine, g1～g2 medicine is compared with blank control group, can be obviously prolonged mice Walking weight load, has significant difference (p ＜ 0.05), the present invention instant integration of edible and medicinal herbs food extracts lyophilized powder g3～g7 Medicine is compared with blank control group, can significantly extend the mice burden swimming time, has pole significant difference (p ＜ 0.01), And it is substantially better than g1～g2 medicine.The results detailed in Table 8.
The impact to the mice burden swimming time for the instant integration of edible and medicinal herbs food extracts lyophilized powder of table 8
" * " p < 0.05vs blank；
" * * " p < 0.01vs blank；
The impact to blood lactase acid before and after mouse movement for the 9.3 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder
After the instant integration of edible and medicinal herbs food extracts lyophilized powder of the orally administration mice present invention, the instant integration of edible and medicinal herbs of the present invention Food extracts lyophilized powder g3～g7 medicine is compared with matched group to blood lactase acid area under curve after mouse movement has statistics poor Different (p ＜ 0.05), though g1～g2 medicine group mice blood lactase acid area under curve is compared with matched group decreasing, has no statistics Learn difference (p ＞ 0.05).The results are shown in Table 9.
The impact to blood lactase acid level before and after mouse movement for the table 9 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder
" * " p < 0.05vs blank；
The impact to mice hepatic glycogen for the 9.4 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder
After orally administration mice g1～g7 medicine, g1～g2 medicine is compared with blank control group, and mice hepatic glycogen content is equal There is obvious rising, there is significant difference (p ＜ 0.05), the present invention instant integration of edible and medicinal herbs food extracts lyophilized powder g3～g7 Medicine compares with blank control group, and mice hepatic glycogen content all has obvious rising, has pole significant difference (p ＜ , and be substantially better than g1～g2 medicine 0.01).The results detailed in Table 10.
The impact to mice hepatic glycogen content for the table 10 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder
" * " p < 0.05vs blank；
" * * " p < 0.01vs blank；
The impact to mice serum carbamide for the 9.5 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder
After orally administration mice g1～g7 medicine, g1～g2 medicine group is compared with blank control group, serum after mouse movement Urea content all has obvious reduction, has significant difference (p ＜ 0.05), and the present invention instant integration of edible and medicinal herbs food extracts freeze Dry powder g3～g7 medicine compares with blank control group, and after mouse movement, serum urea content all has obvious reduction, has pole Significant difference (p ＜ 0.01), and it is substantially better than g1～g2 medicine.The results detailed in Table 11.
The impact to mice serum urea content for the table 11 present invention instant integration of edible and medicinal herbs food extracts lyophilized powder
" * " p < 0.05vs blank；
" * * " p < 0.01vs blank；
10. experiment conclusion
This experiment is mainly tested by mice burden swimming, and the deposit of detection mice hepatic glycogen is fast to observe the present invention simultaneously Molten integration of edible and medicinal herbs food extracts lyophilized powder enhance immunity, the effect of resisting fatigue.Preliminary Results show as follows:
1st, the instant integration of edible and medicinal herbs food extracts lyophilized powder of g3～g7 of the present invention all can extend mice burden swimming time (p ＜ 0.01), and effect is substantially better than the instant integration of edible and medicinal herbs food extracts lyophilized powder of other g1～g2.
2nd, biochemistry detection aspect shows, each dosage group of the instant integration of edible and medicinal herbs food extracts lyophilized powder of g3～g7 of the present invention is equal Lactic acid content produced by glucose anerobic glycolysises in mice serum after moving can be reduced, comparing with matched group has significant difference (p ＜ 0.05), although and the instant integration of edible and medicinal herbs food extracts lyophilized powder of other g1～g2 also can reduce mice blood after motion Lactic acid content produced by middle clearly glucose anerobic glycolysises, but compare with matched group, no difference of science of statistics (p ＞ 0.05)；
3rd, each dosage group of the instant integration of edible and medicinal herbs food extracts lyophilized powder of g3～g7 of the present invention all can significantly improve Mouse Liver The deposit (p ＜ 0.01) of dirty middle glycogen, and effect is substantially better than the instant integration of edible and medicinal herbs food extracts lyophilizing of other g1～g2 Powder；
4th, metabolic arthritis model finds, the instant integration of edible and medicinal herbs food extracts lyophilized powder of g3～g7 of the present invention can significantly reduce little The content (p ＜ 0.01) of urea in serum after Mus swimming, and effect is substantially better than other g1～g2 instant integration of edible and medicinal herbs food and carries Take thing lyophilized powder；
11. conclusions
Above-mentioned experiment proves that the present invention instant integration of edible and medicinal herbs food extracts lyophilized powder can significantly improve immunity of organisms, carries The muscle power of high mice and endurance, reduce the content of urea in serum and lactic acid after mouse movement, and can significantly improve mouse liver The deposit of middle glycogen, contributes to alleviating the fatigue that sports load causes；The time that mice burden swimming to power exhausts can be extended.

Claims (10)

1. a kind of instant integration of edible and medicinal herbs food extracts lyophilized powder, is mainly prepared by the raw material of following parts by weight: integration of edible and medicinal herbs Food 30-50 part, food fibre powder 10-20 part, antifreeze 5-15 part, probiotics factor 5-15 part, Lactobacillus plantarum powder 2-10 part；
Described antifreeze is the plant extract and trehalose, silk prepared for raw material with the natural plants seed containing freeze proof substrate Glue peptide compounds, fully dissolving forms；
The quality of described antifreeze consists of: plant extract: trehalose: sericin peptide taken=37-53:2-8:1-4.
2. as claimed in claim 1 instant integration of edible and medicinal herbs food extracts lyophilized powder it is characterised in that main by following weight The raw material preparation of number: integration of edible and medicinal herbs food 35-45 part, food fibre powder 13-17 part, antifreeze 8-12 part, probiotics factor 8- 12 parts, Lactobacillus plantarum powder 4-8 part.
3. as claimed in claim 1 instant integration of edible and medicinal herbs food extracts lyophilized powder it is characterised in that main by following weight The raw material preparation of number: 40 parts of integration of edible and medicinal herbs food, 15 parts of food fibre powder, 10 parts of antifreeze, 10 parts of probiotics factor, plant breast 6 parts of bacillus powder.
4. described instant integration of edible and medicinal herbs food extracts lyophilized powder as arbitrary in claim 1-3 is it is characterised in that described meals The preparation method of fiber powder, comprises the following steps: will be uniform for inulin, apple fiber, Semen Tritici aestivi fiber 4-6:2-4:1-3 in mass ratio Mixing, micronizing to 3-6 μm of particle diameter, adjustment ground product moisture is 19-21%, in screw speed 160-180r/min, Temperature 185-200 DEG C condition extruding puffing, adds the water of expanded amount of substance 2-4 times, and room temperature 200-400w, 35-40khz condition surpasses Sound extracts 10-15min, then in electric field intensity 20-40kv/cm, burst length 400-600 μ s, and pulse frequency 200-400hz bar Carry out high voltage pulse electric field processing 10-15min under part；Adjusting ph value with lactic acid is 4.5-6.5, adds mixture quality 0.1- 0.3% enzyme, digests 20-48min in 45-55 DEG C；Enzymolysis solution concentrating under reduced pressure, lyophilization obtain final product food fibre powder；
Described enzyme is cellulase, xylanase, laccase, pectase, tannase 2-4:1-3:1-2:0.5- in mass ratio 1.5:0.2-1 uniformly mixes.
5. described instant integration of edible and medicinal herbs food extracts lyophilized powder as arbitrary in claim 1-3 is it is characterised in that described plant The preparation method of extract, comprise the steps: by winter rye, Bulbus Allii Cepae, Sabina vulgaris, Caulis et Folium Ammopiptanthi Mongolici seed 13-17 in mass ratio: 5-11:3-5:1-3 uniformly mixes, sabot, processes 2-4min in electric field intensity 5-7kv/cm high-pressure electrostatic first；Then in concentration For soaking at room temperature 2-4h in the salicylic acid solution of 40-60mg/l, simultaneously in electric field intensity 10-20kv/cm, burst length 150- 250 μ s, carry out high voltage pulse electric field processing under the conditions of pulse frequency 150-250hz；Rinse, drain, stand 18- in 3-5 DEG C 24h, then successively in 1-3 DEG C of cold preservation 2-4d, -3--5 DEG C of freezing 1-3d, -15--18 DEG C of freezing 20-30h, is immediately placed at outdoor In intensity of illumination 0.5-5 ten thousand lx natural lighting 2-4h, compound half is made to be pulverized immediately after thawing, ground product particle diameter 0.5- 1.5mm, is subsequently added into the water of ground product quality 4-6 times, and adjusting ph value with lactic acid is 3.5-5.5；It is eventually adding mixed liquor matter The compound enzyme of amount 1-2%, digests 10-30min in 35-50 DEG C first, then digests 20-40min in 50-60 DEG C；Enzymolysis solution mistake Filter, filtrate reduced in volume obtain final product plant extract to solid content for 15-25%；
Described compound enzyme is cellulase, protease, amylase, pectase, Digestive Enzyme 5-7:2-4:1-3:1-2 in mass ratio: 1-2 uniformly mixes.
6. described instant integration of edible and medicinal herbs food extracts lyophilized powder as arbitrary in claim 1-3 is it is characterised in that described plant Lactobacilluss powder is prepared with Lactobacillus plantarum cgmcc no.11763 according to a conventional method for starting strain, and its viable bacteria content is: 7 ×1012-9×1012cfu/g.
7. as claimed in claim 6 instant integration of edible and medicinal herbs food extracts lyophilized powder it is characterised in that described Lactobacillus plantarum The mass ratio that during powder preparation, wet thallus are mixed with antifreeze is 1:0.6-1.4.
8. described instant integration of edible and medicinal herbs food extracts lyophilized powder as arbitrary in claim 1-7 preparation method it is characterised in that Comprise the steps:
1) preparation of extracting solution: according to formula, weigh each component raw material, put into integration of edible and medicinal herbs food equipped with 0.8- first Clean 3-5min in 200-400w, 20-40khz in the ultrasonic washing unit of 1.2% sodium bicarbonate solution, drain, pretreatment, put In power 3-5kw, thickness of feed layer 2-4cm, 50-70 DEG C in microwave drying, 4-6min is dried, being then immersed in mass percent is 10-15min in the sericin peptide solution of 8-12%, takes out, and is pulverized immediately, freezing after -18--22 DEG C of freezing 30-50min Thickness of feed layer 3-5cm, ground product particle diameter 0.3-0.5mm, it is subsequently added into the water of ground product quality 4-6 times, adjust ph value with lactic acid For 3.5-5.5, at room temperature in electric field intensity 25-35kv/cm, burst length 300-500 μ s, pulse frequency 200-300hz bar Carry out high voltage pulse electric field processing 20-30min under part；Then carry out microwave exposure under the conditions of power 150-300w in room temperature to carry Take 15-20min, simultaneously in power 200-300w, under the conditions of frequency 30-40khz, carry out ultrasonic assistant extraction；Last vacuum takes off It is so incensed that fruit and vegetable juice, adds the mixed enzyme of its quality 0.2-0.6%, digest 30-50min in 40-50 DEG C, take enzymolysis solution quality 0.5- 1% Ovum Gallus domesticus album, puts in blender and stirs 10-12min in rotating speed 800-1000r/min, until Ovum Gallus domesticus album all becomes foam and obtains To egg white foams, egg white foams are added in enzymolysis solution, normal pressure boils 1-3min, is rapidly decreased to room temperature, 80-100 eye mesh screen mistake Filter, filtrate reduced in volume to solid content to 30-50% obtains integration of edible and medicinal herbs food extracting solution；
Described mixed enzyme uniformly mixes for cellulase, protease, pectase, Digestive Enzyme 2-4:1-3:1-2:1-2 in mass ratio；
2) batch mixing: the 4-6% of antifreeze and food fibre powder quality is sequentially added in extracting solution, uniformly mixes, in power 300-500w, carries out ultrasonic dissolution 10-15min under the conditions of frequency 35-45khz, obtains mixed liquor；
3) lyophilizing: extremely -40～-50 DEG C of quick-freezing in dry storehouse put by mixed liquor, and pre-freeze 2～3h at -40～-50 DEG C；Then will After being evacuated to 15～20pa in dry storehouse, progressively heat up and control mixed liquor final temperature in 8-10h at -15～-18 DEG C； Then, in the case that in dry storehouse, vacuum is constant, adjusting temperature in dry storehouse makes it progressively be warming up to room temperature in 5-7h, To lyophilized powder moisture be less than 4% when discharging, with Lactobacillus plantarum powder, remaining food fibre powder, probiotics factor under aseptic condition Uniformly mix to obtain integration of edible and medicinal herbs food extracts lyophilized powder.
9. as claimed in claim 8 the preparation method of instant integration of edible and medicinal herbs food extracts lyophilized powder it is characterised in that described Probiotics factor weight consists of: oligomeric isomaltose 15-25 part, oligomeric galactose 15-25 part, and oligofructose 10-20 part is oligomeric Xylose 10-20 part, stachyose 5-11 part, soybean oligo saccharide 5-11 part, Raffinose 4-10 part, Oligomeric maltose 3-7 part.
10. as claimed in claim 8 the preparation method of instant integration of edible and medicinal herbs food extracts lyophilized powder it is characterised in that institute State integration of edible and medicinal herbs food be Fructus Crataegi, Fructus Lycii, Fructus Jujubae, Fructus Hippophae, Fructus Momordicae, Flos Lonicerae, Poria, Bulbus Lilii, Semen Nelumbinis, any in Radix Puerariae One or more are mixed with arbitrary proportion.
CN201610743227.4A 2016-04-05 2016-08-27 Instant medicinal and edible food extract freeze-dried powder and preparation method thereof Pending CN106360720A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201610206918 2016-04-05
CN2016102069180 2016-04-05

Publications (1)

Publication Number Publication Date
CN106360720A true CN106360720A (en) 2017-02-01

Family

ID=57903578

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610743227.4A Pending CN106360720A (en) 2016-04-05 2016-08-27 Instant medicinal and edible food extract freeze-dried powder and preparation method thereof

Country Status (1)

Country Link
CN (1) CN106360720A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107485025A (en) * 2017-08-31 2017-12-19 广州富诺营养科技有限公司 A kind of Zinc-rich saccharomyces cerevisiae chewable tablets and preparation method thereof
CN108783404A (en) * 2018-06-21 2018-11-13 福建省天源兴达食品有限公司 A kind of preparation method of low-temperature instant agar

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107485025A (en) * 2017-08-31 2017-12-19 广州富诺营养科技有限公司 A kind of Zinc-rich saccharomyces cerevisiae chewable tablets and preparation method thereof
CN108783404A (en) * 2018-06-21 2018-11-13 福建省天源兴达食品有限公司 A kind of preparation method of low-temperature instant agar
CN108783404B (en) * 2018-06-21 2021-09-10 福建天源兴达生物科技有限公司 Preparation method of low-temperature instant agar

Similar Documents

Publication Publication Date Title
CN104509864B (en) A kind of have nutritional health food improving gastrointestinal function and preparation method thereof
CN104872674A (en) Medlar and red date enzyme and preparation method thereof
CN104543679A (en) Probiotic-containing health food and preparation method thereof
CN105795300A (en) Freeze-dried powder of fruit and vegetable extracts and preparation method of freeze-dried powder
CN105455083A (en) Compounded bee pollen and preparation method thereof
CN105707805A (en) Honey cream and preparation method thereof
CN104543680A (en) Health food for resisting fatigue and improving gastrointestinal functions and preparation method of health food
CN105707772A (en) Blueberry composite powder capable of improving performance of intestinal tracts and preparation method of blueberry composite powder
CN105725144A (en) Bee pollen with high wall-breaking rate and preparation method thereof
CN105725123A (en) Blueberry composite powder high in anthocyanin content and preparing method thereof
CN105831666A (en) Probiotic honey composition and preparation method thereof
CN105685913A (en) Honey product and preparation method thereof
CN105707906A (en) Probiotic medicinal and edible food extract freeze-dried powder and preparation method thereof
CN105747236A (en) Extract frozen-dried powder of fruit and vegetable medicine-food-homologous food and preparation method of frozen-dried powder
CN105707803A (en) High-biological-activity bee pollen and preparation method thereof
CN105707802A (en) Digestible bee pollen and preparation method thereof
CN106360720A (en) Instant medicinal and edible food extract freeze-dried powder and preparation method thereof
CN105831645A (en) Probiotic blueberry composite powder and preparation method thereof
CN105768102A (en) Enzyme-containing blueberry composite powder and preparation method thereof
CN105639645A (en) Blueberry composite powder capable of reducing cholesterol and method for preparing blueberry composite powder
CN105707807A (en) Honey composition easy to digest and preparation method thereof
CN105661399A (en) Blueberry composite powder and preparation method thereof
CN105768001A (en) Bee pollen containing probiotics and preparation method thereof
CN105795478A (en) Medicine and food homologous food extract freeze-dried powder with high nutrition value and preparation method of freeze-dried powder
CN105707808A (en) High-stability honey paste and preparation method thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20170201