CN106420847A - Composition for auxiliary protection of gastric mucosa and/or remission of gastrectasia and microecological preparation thereof - Google Patents
Composition for auxiliary protection of gastric mucosa and/or remission of gastrectasia and microecological preparation thereof Download PDFInfo
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- CN106420847A CN106420847A CN201610510429.4A CN201610510429A CN106420847A CN 106420847 A CN106420847 A CN 106420847A CN 201610510429 A CN201610510429 A CN 201610510429A CN 106420847 A CN106420847 A CN 106420847A
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- lactobacillus
- probio
- probiotics
- gasteremphraxis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/745—Bifidobacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Abstract
The present invention belongs to the field of biotechnology and particularly relates to a composition for the auxiliary protection of gastric mucosa and/or the remission of gastrectasia and a microecological preparation thereof. The composition for the auxiliary protection of gastric mucosa and/or the remission of gastrectasia is prepared through the synergistic combination of traditional Chinese medicines homologous in medicine and food, and probiotics. The traditional Chinese medicines homologous in medicine and food are composed of hericium erinaceus and poria cocos. The microecological preparation is prepared by the above composition and preparation auxiliary components. According to the technical scheme of the invention, the composition is prepared through the synergistic combination of traditional Chinese medicines homologous in medicine and food, and probiotics, so that the composition can well protect the gastric mucosal lesion and/or the gastrectasia. Meanwhile, the composition can adjust the balance of intestinal flora and improve the resistance. The traditional Chinese medicines homologous in medicine and food, and the probiotics play effects synergistically and promote each other, thus entirely regulating the body constitution and maintaining the normal health status.
Description
Technical field
The invention belongs to biological technical field and in particular to a kind of auxiliary protection stomach lining and/or alleviate gasteremphraxis combination
Thing and its probiotics.
Background technology
According to one statistics display of the World Health Organization, the incidence of disease in crowd for the stomach trouble is up to 80%, and just with annual
17.43% speed increases.China has nearly 30% people to suffer from various kinds of gastritis, and has 800,000 people to die from cancer of the stomach every year.Chronic stomach
Scorching is to cause stomach lining chronic inflammation by multi-pathogenesis as modal stomach trouble, is digestive system common disease, its incidence of disease is each
Rank first in kind of stomach trouble, account for accepting the 80-90% of gastrocopy patient, the male sex is more than women, with the age increase the incidence of disease by
Cumulative height.
There is its unique microecosystem, because of its special acid environment and strong digestion motion so that only minority in stomach
The bacterium that acid resistance is strong and adhesive force is strong could be colonized, but presents abundant bacterial species.Numerous studies have proven to the stomach of people
Inside there is multiple-microorganism field planting and comprise anaerobic bacteria, facultative anaerobic bacteria and aerobic bacteria etc., bacterial concentration is about 103CFU/mL, its
Middle lactobacillus is important component part.They constitute the specific flora of Tiny ecosystem in stomach, and the change of its type and quantity is direct
The normal physiological function of impact stomach.
China's traditional Chinese medical science is of extensive knowledge and profound scholarship, has been developed for Chinese medicine composition and the traditional Chinese medicine health care product of multiple nourishing the stomach at present, such as applies for
Number disclose a kind of Chinese medicine composition with stomach-nourishing function for 201310639143.2 patent of invention, contain including following weight
The composition of amount:Pawpaw 15-25 part, lotus seeds 10-20 part, Poria cocos 15-25 part, Chinese yam 5-20 part, rhizoma atractylodis 10-20 part, RADIX PAEONIAE ALBA 3-5
Part, cortex magnoliae officinalis 10-15 part.And for example the patent of invention of Application No. 201510972196.5 discloses a kind of Chinese traditional medicine composition of nourishing the stomach
Thing, the ratio of weight and number of each raw material is:Radix Codonopsis 8-15 part, Chinese yam 7-9 part, hawthorn 8-15 part, dried orange peel 6-14 part, bighead atractylodes rhizome 8-15
Part, Radix Glycyrrhizae 3-6 part.
In recent years, probio is increasingly paid close attention to by scholar to the effect of chronic gastritis.Chinese and foreign documents are reported, probio
Multiple acidic materials can be secreted, so that the gastric secretion of mucosa cells is reduced, excessive gastric juice erosion stomach lining will not be produced thin
Born of the same parents, allow cell be in non-fatigue state, thus helping stomach lining reparation.Stomach Helicobacter pylori can be reduced in Gastric Mucosal Cells
Adhesive force, and reduce the activity of stomach Helicobacter pylori urease, gradually decrease its quantity, help adjust gastric acid secretion, solution
Except the symptom of gaseous distention, alleviate the stomach lining inflammation that hyperchylia causes.Probio produces enzyme and vitamin, anti-oxidant,
Mucous membrane sIg A is promoted to generate.
Chinese medicine and probio have his own strong points, but yet there are no and be related to integration of drinking and medicinal herbs Chinese medicine and probio synergistic combination
Effect disquisition.
Content of the invention
In view of this, it is an object of the invention to developing a kind of auxiliary protection stomach lining and/or the composition of alleviation gasteremphraxis,
Said composition is formed with probio synergistic combination by integration of drinking and medicinal herbs Chinese medicine, mucosal lesion and/or gasteremphraxis is had preferable protection make
With can also adjust gut flora balance, improve resistance simultaneously.
For achieving the above object, the technical scheme is that:
Auxiliary protection stomach lining and/or the composition alleviating gasteremphraxis, by integration of drinking and medicinal herbs Chinese medicine and probio synergistic combination
Become, described integration of drinking and medicinal herbs Chinese medicine is made up of Hericium erinaceus and Poria cocos, described probio is by lactobacillus reuteri LY0032, plant breast bar
Bacterium ZW0045 and lactobacillus gasseri GS0010 composition, described lactobacillus reuteri LY0032 deposit number is CCTCC NO:M
2015341, described Lactobacillus plantarum ZW0045 deposit number is CCTCC NO:M 2015127, described lactobacillus gasseri GS0010
Deposit number is CCTCC NO:M 2015340.
Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, lactobacillus gasseri GS0010 have good protection stomach
Mucous membrane function.
Lactobacillus reuteri LY0032, is deposited in China typical culture collection center, address on May 29th, 2015
For Wuhan, China Wuchang Luo Jia Shan Wuhan University China typical culture collection center in the school, deposit number is CCTCC NO:M
2015341.Its Classification And Nomenclature:Lactobacillus reuteri BLCC2-0032, Latin title:Lactobacillus reuteri
BLCC2-0032.
Lactobacillus plantarum ZW0045, is deposited in China typical culture collection center on March 18th, 2015, address is
Wuhan, China Wuchang Luo Jia Shan Wuhan University China typical culture collection center in the school, deposit number is CCTCC NO:M
2015127.Its Classification And Nomenclature:Lactobacillus plantarum BLCC2-0045, Latin title:Lactobacillus plantarum
BLCC2-0045.
Lactobacillus gasseri GS0010, is deposited in China typical culture collection center on May 29th, 2015, address is
Wuhan, China Wuchang Luo Jia Shan Wuhan University China typical culture collection center in the school, deposit number is CCTCC NO:M
2015340.Its Classification And Nomenclature:Lactobacillus gasseri 0010, Latin title:Lactobacillus gasseri 0010.
Hericium erinaceus flat property and sweet taste, favourable the five internal organs, aid digestion, the effects such as nourish the body;Clinical practice shows, Hericium erinaceus can be controlled
Treat the diseases such as indigestion, gastric ulcer, antral gastritis, stomachache, gasteremphraxis.Poria cocos is sweet flat, dampness removing thermal detoxification, strengthening the spleen and stomach.Both are same
Clothes can play effect of invigorating the spleen, nourishing the stomach.
Common probio has lactic acid bacteria and Bifidobacterium, and probio is the class active microorganism beneficial to host, is
It is colonized in human body intestinal canal, in reproductive system, definite health efficacy can be produced thus improving host's microecological balance, playing beneficial work
Active beneficial microbe.Described lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, lactobacillus gasseri GS0010 exist
Remain to keep greater activity in the experiment of acidproof, bile tolerance, through zoopery checking and human body examination take experiment have protection stomach lining,
Alleviate the effect of gasteremphraxis.Integration of drinking and medicinal herbs Chinese medicine is acted synergistically with probio, mutually promotes, integrally-regulated body physique, just safeguards
Often health status.
Further, described composition, described integration of drinking and medicinal herbs Chinese medicine is by weight by Hericium erinaceus 20-35 part, Poria cocos 5-15
Part composition.
Further, described composition, described lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, grignard breast bar
The ratio of bacterium GS0010 tri- bacterium is 1-2:1-2:1-2.
Further it is preferred that described lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, lactobacillus gasseri
The ratio of GS0010 tri- bacterium is 1:1:2.
Further, described composition, described probio also include bifidobacterium lactis, lactobacillus acidophilus, bifidobacterium longum,
One or more of Lactobacillus rhamnosus;Described lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045 and grignard breast bar
The viable count of bacterium GS0010 tri- bacterium accounts for more than the 80% of probio total bacteria count.Bifidobacterium lactis, lactobacillus acidophilus, long bifid bar
A few plant height of bacterium, Lactobacillus rhamnosus effect function probiotic strain enter human body after can in enteron aisle large number of viable, play regulation intestines
Interior colony balance, promotion human consumption absorb, and more preferably promote the absorption of integration of drinking and medicinal herbs Effective Component of Chinese Medicine.Strengthen host couple simultaneously
The nonspecific resistance of microbial pathogens.Its metabolite can promote the propagation of the beneficial bacteriums such as Bifidobacterium, promotes stomach and intestine
Wriggling and the discharge of stool.
Integration of drinking and medicinal herbs Chinese medicine is acted synergistically with probio, mutually promotes, and integrally-regulated body physique safeguards normal health shape
State.Therefore, the present invention is in addition to protection said composition, also protect described composition prepare auxiliary protection stomach lining and/or
Application in the pharmaceutical preparation of alleviation gasteremphraxis and/or health products.
The Tiny ecosystem that the present invention also provides a kind of specific auxiliary protection stomach lining on this basis and/or alleviates gasteremphraxis
Preparation, is prepared from by composition of the present invention and preparation auxiliary element.This probiotics can be that this area is any
Acceptable formulation, such as pulvis, tablet, capsule etc..
Preferably, described probiotics, contains 10 in every gram of probiotics9-1011The probio of CFU.
Preferably, described probiotics, described preparation auxiliary element is oligoisomaltose, xylitol, milk powder, battalion
One or more of foster yeast and fruit powder.
Present invention also offers a kind of preparation method of probiotics, specifically include the step being carried out as follows:
(1) each probio is prepared into lyophilized bacterium powder standby;
(2) Chinese medicine is pulverized respectively, then mix, obtain Traditional Chinese drug mixture;
(3) take the lyophilized bacterium powder mixing of each probio in step (1), be subsequently adding the Traditional Chinese drug mixture described in step (2)
In, then add preparation auxiliary element, it is sufficiently mixed and obtain final product probiotics.
In the method for the present invention, each probio is prepared into lyophilized bacterium powder with reference to any conventional practices in this area.
Preferably, described preparation method, in described step (1), it is also possible to use protection in the preparation process of lyophilized bacterium powder
Agent, described protective agent can be using commonly used any protective agent in this area.
Preferably, described protective agent is composed of the following components by weight:10% skimmed milk power 70-90 part, sucrose 1-
15 parts, glycerine 0.1-5 part.
Preferably, described preparation method, in described step (2), after pulverizing, particle diameter is less than 50um.
The beneficial effects of the present invention is:
(1) a kind of auxiliary protection stomach lining of the present invention and/or the composition alleviating gasteremphraxis, said composition is by integration of drinking and medicinal herbs
Chinese medicine is formed with probio synergistic combination, product preferably three probiotics bacterial strain, has preferable guarantor to mucosal lesion and/or gasteremphraxis
Shield acts on, and can also adjust gut flora balance simultaneously, improves resistance.By integration of drinking and medicinal herbs Chinese medicine and probio coordinated groups cooperation
With, while adjusting lifting immunity, mutually promote, integrally-regulated body physique, safeguard normal healthy state.
(2) auxiliary protection stomach lining of the present invention and/or the probiotics alleviating gasteremphraxis, including integration of drinking and medicinal herbs Chinese medicine and benefit
Raw bacterium, auxiliary element oligoisomaltose, xylitol, milk powder, nutritious yeast and fruit powder in product, prebiotics promotes probio
Propagation, meets body part nutritional need simultaneously, lifts mouthfeel.Through animal experiment checking, the mucosal lesion that alcohol is caused
And antibiotic causes gasteremphraxis to have preferable protective effect, try clothes proof through human body square to alleviation stomachache gasteremphraxis, rush digestion increasing appetite etc.
There is good effect in face;Can also adjust gut flora balance simultaneously, improve resistance;And safety, long shelf-life, have wide
Market prospects.The present invention can be any acceptable formulation in this area, such as pulvis, tablet, capsule etc., convenient, portable,
And safety, long shelf-life, there are wide market prospects.
(3) method that the present invention prepares probiotics, simple to operate it is easy to industrialized production.
Brief description
Fig. 1 mouse mucosal lesion situation audio-visual picture.
Fig. 2 mouse stomach lining slice map.
Fig. 3 mouse gasteremphraxis situation audio-visual picture.
Fig. 4 human body examination clothes checking feedback result.
Specific embodiment
Illustrated embodiment is to preferably illustrate to present disclosure, but is not that present disclosure only limits
In illustrated embodiment.So those of ordinary skill in the art carry out nonessential changing according to foregoing invention content to embodiment
Enter and adjust, also include any combination between each specific embodiment, still fall within protection scope of the present invention.
The application of the composition of Part I auxiliary protection of the present invention stomach lining and/or alleviation gasteremphraxis
Embodiment 1 auxiliary protection stomach lining and/or the probiotics alleviating gasteremphraxis
Auxiliary protection stomach lining and/or the probiotics alleviating gasteremphraxis, including integration of drinking and medicinal herbs Chinese medicine and probio, by weight
Amount part meter is specifically composed of the following components:
25 parts of Hericium erinaceus;
10 parts of Poria cocos;
21 parts of oligoisomaltose;
17 parts of xylitol;
25 parts of milk powder;
It is mixed with 2.5 × 10 in wherein every gram composition10The probio of CFU;Described probio is by lactobacillus reuteri
LY0032, Lactobacillus plantarum ZW0045 and lactobacillus gasseri GS0010 composition, described lactobacillus reuteri LY0032 deposit number
For CCTCC NO:M 2015341, described Lactobacillus plantarum ZW0045 deposit number is CCTCC NO:M 2015127, described lattice
Family name's lactobacillus GS0010 deposit number is CCTCC NO:M 2015340.
Embodiment 2 auxiliary protection stomach lining and/or the probiotics alleviating gasteremphraxis
Auxiliary protection stomach lining and/or the probiotics alleviating gasteremphraxis, including integration of drinking and medicinal herbs Chinese medicine and probio, by weight
Amount part meter is specifically composed of the following components:
25 parts of Hericium erinaceus;
10 parts of Poria cocos;
21 parts of oligoisomaltose;
17 parts of xylitol;
25 parts of milk powder;
It is mixed with 4.5 × 10 in wherein every gram composition9The probio of CFU;Described probio is by lactobacillus reuteri
LY0032, Lactobacillus plantarum ZW0045 and lactobacillus gasseri GS0010 composition, described lactobacillus reuteri LY0032 deposit number
For CCTCC NO:M 2015341, described Lactobacillus plantarum ZW0045 deposit number is CCTCC NO:M 2015127, described lattice
Family name's lactobacillus GS0010 deposit number is CCTCC NO:M 2015340;Described lactobacillus reuteri LY0032, Lactobacillus plantarum
ZW0045, the ratio of lactobacillus gasseri GS0010 tri- bacterium are 1:1:2.
Embodiment 3 auxiliary protection stomach lining and/or the probiotics alleviating gasteremphraxis
Auxiliary protection stomach lining and/or the probiotics alleviating gasteremphraxis, including integration of drinking and medicinal herbs Chinese medicine and probio, by weight
Amount part meter is specifically composed of the following components:
25 parts of Hericium erinaceus;
10 parts of Poria cocos;
15 parts of oligoisomaltose;
12 parts of xylitol;
20 parts of milk powder;
5 parts of nutritious yeast;
13 parts of fruit powder etc.;
It is mixed with 6.5 × 10 in wherein every gram composition9The probio of CFU;Described probio is by lactobacillus reuteri
LY0032, Lactobacillus plantarum ZW0045, lactobacillus gasseri GS0010 composition, bifidobacterium lactis, lactobacillus acidophilus composition, described sieve
Yi Shi lactobacillus LY0032 deposit number is CCTCC NO:M 2015341, described Lactobacillus plantarum ZW0045 deposit number is
CCTCC NO:M 2015127, described lactobacillus gasseri GS0010 deposit number is CCTCC NO:M 2015340;Described Roy
The viable count of family name lactobacillus LY0032, Lactobacillus plantarum ZW0045 and lactobacillus gasseri GS0010 tri- bacterium accounts for probio total bacteria count
80%, remaining is supplied by bifidobacterium lactis and lactobacillus acidophilus;Described lactobacillus reuteri LY0032, Lactobacillus plantarum
ZW0045, the ratio of lactobacillus gasseri GS0010 tri- bacterium are 2:1:2, the ratio of described bifidobacterium lactis and lactobacillus acidophilus is 1:
1.
Embodiment 4 auxiliary protection stomach lining and/or the probiotics alleviating gasteremphraxis
Auxiliary protection stomach lining and/or the probiotics alleviating gasteremphraxis, including integration of drinking and medicinal herbs Chinese medicine and probio, by weight
Amount part meter is specifically composed of the following components:
25 parts of Hericium erinaceus;
10 parts of Poria cocos;
15 parts of oligoisomaltose;
12 parts of xylitol;
20 parts of milk powder;
5 parts of nutritious yeast;
13 parts of fruit powder etc.;
It is mixed with 6.5 × 10 in wherein every gram composition9The probio of CFU;Described probio is by lactobacillus reuteri
LY0032, Lactobacillus plantarum ZW0045, lactobacillus gasseri GS0010, bifidobacterium lactis, lactobacillus acidophilus, bifidobacterium longum, mouse
Lee's sugar lactobacillus composition;Described lactobacillus reuteri LY0032 deposit number is CCTCC NO:M 2015341, described plant breast
Bacillus ZW0045 deposit number is CCTCC NO:M 2015127, described lactobacillus gasseri GS0010 deposit number is CCTCC
NO:M 2015340;Described lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045 and lactobacillus gasseri GS0010 tri- bacterium
Viable count accounts for the 85% of probio total bacteria count, and remaining is by bifidobacterium lactis, lactobacillus acidophilus, bifidobacterium longum, rhamnose breast bar
Bacterium is supplied;Described lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, the ratio of lactobacillus gasseri GS0010 tri- bacterium are 1:
1:2, described bifidobacterium lactis, lactobacillus acidophilus, bifidobacterium longum, the ratio of Lactobacillus rhamnosus are 1:1:1:1.
The preparation method of the probiotics of embodiment 5 auxiliary protection stomach lining and/or alleviation gasteremphraxis
The preparation method of the present invention to be described taking the probiotics of embodiment 4 as a example.
Probiotics strain has lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, lactobacillus gasseri GS0010, breast double
Discrimination bacillus, lactobacillus acidophilus, bifidobacterium longum and Lactobacillus rhamnosus.In the present invention, in probio, the culture of lactobacillus is lived
Change condition is:Cultivate 22-26h for 25-40 DEG C, the condition of first order seed culture is:Under the conditions of 25-40 DEG C, quiescent culture 12-24h,
The condition of fermented and cultured is:Under the conditions of 25-40 DEG C, intermittent oscillation cultivates 48-96h, and mixing speed is intermittent stirring 100r/min,
5min/2h;In probio, the culture activation condition of Bifidobacterium is:25-40 DEG C of culture 22-26h, the condition of first order seed culture
For:32-40 DEG C of Anaerobic culturel 20-28h, seed culture fluid condition of culture is:Quiescent culture 16-30h under the conditions of 32-40 DEG C, sends out
Ferment condition of culture is:28-40 DEG C, initial pH value is 6.0-7.0, and fermentation time is 48-96h.
In probio, the culture medium of the culture activation of lactobacillus is MRS solid medium, primary-seed medium and fermentation
Culture medium is the MRS fluid nutrient medium without agar;In probio, the culture activation medium of Bifidobacterium is trained for TPY solid
Foster base, primary-seed medium and fermentation medium are the TPY fluid nutrient medium without agar.
The specific method of the present embodiment is as follows:
1st, each probio is prepared into lyophilized bacterium powder
(1) configure culture medium
MRS solid culture based formulas are:Glucose 2.0%, ammonium citrate 0.2%, sodium acetate 0.5%, dipotassium hydrogen phosphate
0.5%th, manganese sulfate 0.02%, magnesium sulfate 0.05%, peptone 1.0%, beef extract 1.0%, yeast extract 0.5%, twen-80
0.1%, 1.5% agar powder (import), adjust pH6.0.Fluid nutrient medium is the solid culture based formulas without agar.
TPY solid culture based formulas are:Casein peptone 1%, soy peptone 0.5%, dusty yeast 0.25%, phosphoric acid hydrogen two
Potassium 0.2%, MgCl26H2O 0.05%, calcium chloride 0.015%, ZnSO4 7H2O 0.025%, iron chloride 0.01%, L- half
Cystine hydrochloride 0.005%, glucose 1.5%, Tween-80 1mL (1000mL adds 1mL), 1.5% agar powder (import), adjust
Section pH to 6.3-6.6, sterilize under the conditions of 115 DEG C 20min.Fluid nutrient medium is the solid culture based formulas without agar.
(2) culture of probio
By lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, lactobacillus gasseri GS0010, lactobacillus acidophilus, mouse
Lee's sugar lactobacillus is respectively connected in MRS solid slope culture medium, 37 DEG C of culture 24h;Then it is transferred in MRS fluid nutrient medium,
37 DEG C of culture 24h, prepared primary seed solution;Then transferred with 5% inoculum concentration again and carry out High Density Cultivation into fermentation medium,
Fermentation condition is 37 DEG C of interval concussion 72h.
Bifidobacterium lactis, bifidobacterium longum are respectively connected in TPY solid slope culture medium, 37 DEG C of culture 24h;Then turn
It is connected in TPY fluid nutrient medium, 37 DEG C of quiescent culture 24h, prepared primary seed solution;Then again with 5% inoculum concentration transfer into
Fermentation medium carries out High Density Cultivation, and fermentation condition is 37 DEG C of standing 72h.
(3) prepare lyophilized bacterium powder
Treat that in zymotic fluid, cell density reaches 109Cfu/mL, collects zymotic fluid.Acquisition thalline be collected by centrifugation, add by
The protective agent that 10% skimmed milk power 91%, sucrose 8%, glycerine 1% are constituted, is lyophilized into bacterium powder.
2nd, Chinese medicine is pulverized respectively, sieve, obtain the traditional Chinese medicine powder that particle diameter is less than 50um, weigh weight in proportion, then mix
Close, obtain Traditional Chinese drug mixture;
3rd, lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, lactobacillus gasseri GS0010 are taken, according to viable count 1:
1:2 ratio adds to Traditional Chinese drug mixture after compounding, and is subsequently adding each auxiliary material, mixes, lactobacillus reuteri LY0032,
Lactobacillus plantarum ZW0045, tri- plants of bacterium viable counts of lactobacillus gasseri GS0010 account for more than the 80% of total viable count, then take breast double
Discrimination bacillus, lactobacillus acidophilus, bifidobacterium longum, Lactobacillus rhamnosus, according to viable count 1:1:1:1 ratio is mixed after compounding
Close bacterium powder, supplied with bifidobacterium lactis, lactobacillus acidophilus, bifidobacterium longum, Lactobacillus rhamnosus mixing bacterium powder, mix i.e.
Obtain probiotics product, the wherein total viable count of probio reaches 2.5 × 1010cfu/g.
The composition integration of drinking and medicinal herbs Chinese medicine of the Part II present invention and probio synergy research
Embodiment 6 ethanol causes mouse mucosal lesion animal pattern experimental verification
(1) experimentation
Take male mice 40, random point 4 groups, 4 groups are respectively designated as CK (control group), MX (ethanol cause mouse stomach lining
Damage model animal), J123 (equal proportion LY0032, ZW0045 and GS0010 mixing), (probiotics, in J123+ for J+Y
Medicine), every group of mouse according to giving relative medicine gavage shown in table 1,1 time/d, gavage 10 days.
Table 1 zoopery packet and operational circumstances
Model successfully judgement property index:
Morphologic observation index includes the general condition such as stomach size, wall of stomach thickness, pleat quantity, mucous membrane color and luster and bleeding.
Normal group stomach lining is in light red, smooth surface, the many mucus of attachment, and holostrome coat of the stomach is thicker, pleat rule, high resilience.Mould
Type group mucous membrane color is pale, and coat of the stomach elasticity is poor, and pleat is flat or moves towards disorderly, and mucous layer is thinning, adheres to a small amount of mucus, part stomach
Mucous membrane occurs blutpunkte.
Histopathology detects:The visible mucous membrane of model group is damaged, tissue loss.Control group stomach lining is complete, no substantially takes off
Fall.
Before last dose, water 24h is can't help in fasting, and after administration, 1h carries out gavage absolute ethyl alcohol 0.2mL/ and only processes, and control group is used
Physiological saline substitutes absolute ethyl alcohol.After 3h, weigh, cervical dislocation is put to death, and wins full stomach, cuts off, physiological saline along at greater curvature
Rinse, observe mouse mucosal lesion situation and carry out formaldehyde fix in case biopsy tissues observe.
(2) experimental result
Mouse stomach lining observes result as shown in figure 1, compared with control group (CK), model group (MX) mucosal lesion is tighter
Weight, composite bacterium powder group (J123) is more notable to the protective effect of stomach lining with product group (J+Y).Stomach lining section result is also filled
Divide and demonstrate this conclusion, such as Fig. 2.Illustrate that the probiotics product of the present invention has preferably to the mucosal lesion that alcohol causes
Protective effect, that is, the composition integration of drinking and medicinal herbs Chinese medicine of the present invention and probio have synergy.
Embodiment 7 antibiotic causes mouse gasteremphraxis animal pattern experimental verification
(1) experimentation
Take male mice 40, random point 4 groups, 4 groups are respectively designated as CK (control group), MX (antibiotic cause mouse gasteremphraxis
Animal pattern), JF (equal proportion LY0032, ZW0045 and GS0010 mixing), JY (probiotics product, JF+ Chinese medicine).Every group
Mouse gives relative medicine gavage, 1 time/d, gavage 10 days.Concrete packet and operation are as shown in table 2.
Table 2 zoopery packet and operational circumstances
In addition to control group physiological saline, 1-4 days every group all gavaged with Lincomycin Hydrochloride, the 5th day starts, and model group continues
Continuous Lincomycin Hydrochloride gavage, experimental group is accordingly intervened, and after last dose 3h, weighs, and cervical dislocation is put to death, and opens abdominal cavity
Win full stomach, open abdominal cavity and win full stomach, observe mouse gasteremphraxis situation.
Model successfully judgement property index:
Observe and include stomach size, oedema situation.The full stomach of normal group is less, in light color, high resilience.The full stomach of model group is relatively
Greatly, color is deeper, oedema, and coat of the stomach elasticity is poor, relatively thin.
(2) experimental result
After off-test, mouse gasteremphraxis situation is as shown in figure 3, gasteremphraxis level data is as shown in table 3.Compared with model group,
The mouse gasteremphraxis that probiotic group and product group all cause to antibiotic has mitigation.
Table 3 gasteremphraxis level data
The probiotics application effect research of the Part III present invention
The application (human body examination clothes) of embodiment 8 probiotics
For verifying probiotics effect in auxiliary protection stomach lining and alleviation gasteremphraxis of the present invention, screening stomach is frequent
10 uncomfortable people participate in product examination clothes, receive 9 parts of feedback forms.Through 10d try clothes, feedback display prescription to alleviate stomachache gasteremphraxis,
Promoting the aspects such as digestion increasing appetite has good effect, and result is as shown in Figure 4.
The probiotics of the present invention tries clothes proof through human body to be had very to alleviating the aspects such as stomachache gasteremphraxis, rush digestion increasing appetite
Good effect;In addition, due to the addition of probio, can also adjust gut flora balance, improve resistance, have wide
Market prospects.
Finally illustrate, above example only in order to technical scheme to be described and unrestricted, although with reference to relatively
Good embodiment has been described in detail to the present invention, it will be understood by those within the art that, can be to the skill of the present invention
Art scheme is modified or equivalent, the objective without deviating from technical solution of the present invention and scope, and it all should be covered at this
In the middle of the right of invention.
Claims (10)
1. the composition of auxiliary protection stomach lining and/or alleviation gasteremphraxis is it is characterised in that assisted with probio by integration of drinking and medicinal herbs Chinese medicine
With combining, described integration of drinking and medicinal herbs Chinese medicine is made up of Hericium erinaceus and Poria cocos, described probio by lactobacillus reuteri LY0032,
Lactobacillus plantarum ZW0045 and lactobacillus gasseri GS0010 composition, described lactobacillus reuteri LY0032 deposit number is CCTCC
NO:M 2015341, described Lactobacillus plantarum ZW0045 deposit number is CCTCC NO:M 2015127, described lactobacillus gasseri
GS0010 deposit number is CCTCC NO:M 2015340.
2. composition according to claim 1 is it is characterised in that described integration of drinking and medicinal herbs Chinese medicine is by weight by Hericium erinaceus
20-35 part, Poria cocos 5-15 part composition.
3. composition according to claim 1 is it is characterised in that described lactobacillus reuteri LY0032, Lactobacillus plantarum
ZW0045, the ratio of lactobacillus gasseri GS0010 tri- bacterium are 1-2:1-2:1-2.
4. composition according to claim 1 is it is characterised in that described probio also includes bifidobacterium lactis, acidophilus breast
One or more of bacillus, bifidobacterium longum, Lactobacillus rhamnosus;Described lactobacillus reuteri LY0032, Lactobacillus plantarum
The viable count of ZW0045 and lactobacillus gasseri GS0010 tri- bacterium accounts for more than the 80% of probio total bacteria count.
5. the composition described in any one of claim 1-4 is in the medicine system preparing auxiliary protection stomach lining and/or alleviate gasteremphraxis
Application in agent and/or health products.
6. the probiotics of a kind of auxiliary protection stomach lining and/or alleviation gasteremphraxis is it is characterised in that appointed by claim 1-4
Composition described in one and preparation auxiliary element are prepared from.
7. probiotics according to claim 6 is it is characterised in that contain 10 in every gram of probiotics9-1011CFU
Probio.
8. probiotics according to claim 6 is it is characterised in that described preparation auxiliary element is oligomeric different malt
One or more of sugar, xylitol, milk powder, nutritious yeast and fruit powder.
9. the preparation method of the probiotics described in claim 6 is it is characterised in that include the step being carried out as follows:
(1) each probio is prepared into lyophilized bacterium powder standby;
(2) Chinese medicine is pulverized respectively, then mix, obtain Traditional Chinese drug mixture;
(3) take the lyophilized bacterium powder mixing of each probio in step (1), be subsequently adding in the Traditional Chinese drug mixture described in step (2), then
Add preparation auxiliary element, be sufficiently mixed and obtain final product probiotics.
10. preparation method according to claim 9 is it is characterised in that in described step (1), the protective agent of lyophilized bacterium powder
Composed of the following components by weight:10% skimmed milk power 70-90 part, sucrose 1-15 part, glycerine 0.1-5 part.
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