CN106420847B - Composition for assisting in protecting gastric mucosa and/or relieving gastrectasia and microecological preparation thereof - Google Patents

Abstract

The invention belongs to the technical field of biology, and particularly relates to a composition for assisting in protecting gastric mucosa and/or relieving gastrectasia and a microecological preparation thereof. The composition for assisting in protecting gastric mucosa and/or relieving gastrectasia is formed by synergistically combining medicinal and edible traditional Chinese medicines and probiotics, wherein the medicinal and edible traditional Chinese medicines comprise hericium erinaceus and poria cocos, and the probiotics comprise lactobacillus reuteri LY0032, lactobacillus plantarum ZW0045 and lactobacillus gasseri GS 0010. The microecological preparation is prepared from the composition and preparation auxiliary components. The composition is prepared by combining medicinal and edible traditional Chinese medicines and probiotics synergistically, has a good protection effect on gastric mucosa injury and/or gastrectasia, and can also adjust the balance of intestinal flora and improve the resistance. The medicine and food homologous traditional Chinese medicine and the probiotics act synergistically to promote each other, integrally regulate the body constitution and maintain the normal health state.

Description

Composition for assisting protection of gastric mucosa and/or relieving gastric distention and its microecological preparation

technical field

The invention belongs to the field of biotechnology, and in particular relates to a composition for assisting the protection of gastric mucosa and/or relieving gastric distension and a microecological preparation thereof.

Background technique

According to a statistics from the World Health Organization, the incidence of gastric disease in the population is as high as 80%, and it is increasing at a rate of 17.43% every year. Nearly 30% of people in my country suffer from various types of gastritis, and 800,000 people die of gastric cancer every year. As the most common gastric disease, chronic gastritis is a chronic inflammation of the gastric mucosa caused by multiple etiologies. It is a common disease of the digestive system. Its incidence ranks first among various gastric diseases, accounting for about 80-90% of patients undergoing gastroscopy. Males are more than females. The incidence increases with age.

There is a unique micro-ecosystem in the stomach. Due to its special acid environment and strong digestive movement, only a few bacteria with strong acid resistance and strong adhesion can colonize, but there are abundant bacterial species. A large number of studies have confirmed that there are a variety of microbial colonization in the human stomach, including anaerobic bacteria, facultative anaerobic bacteria and aerobic bacteria, and the bacterial concentration is about 10 ³ CFU/mL, of which Lactobacillus is an important part. They constitute the specific flora of the microecology in the stomach, and the changes in their types and quantities directly affect the normal physiological function of the stomach.

my country's traditional Chinese medicine is extensive and profound. At present, a variety of traditional Chinese medicine compositions and traditional Chinese medicine health care products for nourishing the stomach have been developed. For example, the invention patent with the application number of 201310639143.2 discloses a traditional Chinese medicine composition with the function of nourishing the stomach, including the following components by weight: papaya 15-25 parts, lotus seeds 10-20 parts, Poria 15-25 parts, Chinese yam 5-20 parts, Atractylodes 10-20 parts, Bai Shaoyao 3-5 parts, Chuan Pu 10-15 parts. Another example is the invention patent whose application number is 201510972196.5, which discloses a traditional Chinese medicine composition for nourishing the stomach. 14 parts, Atractylodes 8-15 parts, Licorice 3-6 parts.

In recent years, the effect of probiotics on chronic gastritis has attracted more and more attention of scholars. Chinese and foreign literatures report that probiotics can secrete a variety of acidic substances, reduce the secretion of gastric juice by mucosal cells, and prevent excessive gastric juice from eroding gastric mucosal cells, leaving cells in a non-fatigue state, thereby helping gastric mucosal repair. It can reduce the adhesion of gastric Helicobacter pylori to gastric mucosal cells, reduce the activity of gastric Helicobacter pylori urease, gradually reduce its number, help adjust gastric acid secretion, relieve the symptoms of flatulence, and relieve gastric mucosal inflammation caused by excessive gastric juice. . Probiotics produce enzymes and vitamins, antioxidants, and promote mucosal sIg A production.

Traditional Chinese medicine and probiotics have their own advantages, but there is no research on the effect of synergistic combination of traditional Chinese medicine and probiotics.

SUMMARY OF THE INVENTION

In view of this, the object of the present invention is to develop a composition for assisting the protection of gastric mucosa and/or relieving gastric bloating. Stomach bloating has a good protective effect, and can also adjust the balance of intestinal flora and improve resistance.

For achieving the above object, the technical scheme of the present invention is:

The composition for assisting in protecting gastric mucosa and/or relieving stomach bloating is formed by synergistic combination of traditional Chinese medicine and probiotics, the traditional Chinese medicine is composed of Hericium erinaceus and Poria, the probiotic Lactobacillus plantarum LY0032, Lactobacillus plantarum ZW0045 and Lactobacillus gasseri GS0010, the Lactobacillus reuteri LY0032 deposit number is CCTCC NO: M2015341, the Lactobacillus plantarum ZW0045 deposit number is CCTCC NO: M 2015127, the Lactobacillus gasseri GS0010 deposit number is CCTCC NO: M 2015340.

Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, and Lactobacillus gasseri GS0010 have a good function of protecting gastric mucosa.

Lactobacillus reuteri LY0032 has been deposited in the China Center for Type Culture Collection on May 29, 2015. The address is the Chinese Type Culture Collection Center in Wuhan University, Luojia Mountain, Wuchang, Wuhan, China. The deposit number is CCTCC NO: M2015341. Its classification name: Lactobacillus reuteri BLCC2-0032, Latin name: Lactobacillus reuteriBLCC2-0032.

Lactobacillus plantarum ZW0045 has been deposited in the China Center for Type Culture Collection on March 18, 2015. The address is the China Type Culture Collection Center in Wuhan University, Luojia Mountain, Wuchang, Wuhan, China. The deposit number is CCTCC NO: M2015127. Its classification name: Lactobacillus plantarum BLCC2-0045, Latin name: Lactobacillus plantarumBLCC2-0045.

Lactobacillus gasseri GS0010 has been deposited in the China Center for Type Culture Collection on May 29, 2015. The address is the China Type Culture Collection Center in Wuhan University, Luojia Mountain, Wuchang, Wuhan, China. The deposit number is CCTCC NO: M2015340. Its classification name: Lactobacillus gasseri 0010, Latin name: Lactobacillus gasseri 0010.

Hericium erinaceus is flat and sweet in taste, beneficial to the five internal organs, aids digestion, nourishes the body and other effects; clinical applications show that Hericium erinaceus can treat indigestion, gastric ulcer, antral gastritis, stomach pain, stomach bloating and other diseases. Poria is sweet in nature, relieves dampness and heat and detoxifies, strengthens the spleen and stomach. Taking the two together can strengthen the spleen and nourish the stomach.

Common probiotics include lactic acid bacteria and bifidobacteria. Probiotics are a type of active microorganisms that are beneficial to the host. They are colonized in the human intestine and reproductive system and can produce exact health effects to improve the host's micro-ecological balance and play a beneficial role. Active beneficial microorganisms. The Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, and Lactobacillus gasseri GS0010 can still maintain high activity in acid resistance and bile salt resistance experiments, and have been verified by animal experiments and human trials. swelling effect. Medicinal and food homologous traditional Chinese medicine and probiotics work synergistically, promote each other, regulate the body's constitution as a whole, and maintain a normal health state.

Further, in the composition, the medicinal and food homologous Chinese medicine is composed of 20-35 parts of Hericium erinaceus and 5-15 parts of Poria by weight.

Further, in the composition, the ratio of Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, and Lactobacillus gasseri GS0010 is 1-2:1-2:1-2.

Further, preferably, the ratio of Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, and Lactobacillus gasseri GS0010 is 1:1:2.

Further, in the composition, the probiotics also include one or more of Bifidobacterium lactis, Lactobacillus acidophilus, Bifidobacterium longum, and Lactobacillus rhamnosus; the Lactobacillus reuteri LY0032 , Lactobacillus plantarum ZW0045 and Lactobacillus gasseri GS0010 the number of viable bacteria accounted for more than 80% of the total number of probiotic bacteria. Bifidobacterium lactis, Lactobacillus acidophilus, Bifidobacterium longum, and Lactobacillus rhamnosus several high-efficiency and functional probiotic strains can survive in large quantities in the intestinal tract after entering the human body, which can regulate the balance of intestinal flora and promote human digestion and absorption. It can better promote the absorption of the active ingredients of traditional Chinese medicine with the same source of medicine and food. Simultaneously enhance the host's non-specific resistance to microbial pathogens. Its metabolites can promote the proliferation of beneficial bacteria such as bifidobacteria, and promote gastrointestinal motility and excretion of stool.

Medicinal and food homologous traditional Chinese medicine and probiotics work synergistically, promote each other, regulate the body's constitution as a whole, and maintain a normal health state. Therefore, the present invention not only protects the composition, but also protects the application of the composition in the preparation of pharmaceutical preparations and/or health care products for assisting in protecting gastric mucosa and/or relieving gastric bloating.

On this basis, the present invention also provides a specific microecological preparation for assisting the protection of gastric mucosa and/or relieving gastric distention, which is prepared from the composition and preparation auxiliary components of the present invention. The probiotics can be in any acceptable dosage form in the art, such as powder, tablet, capsule and the like.

Preferably, the probiotics contain 10 ⁹ -10 ¹¹ CFU of probiotics per gram of the probiotics.

Preferably, in the probiotic preparation, the auxiliary components of the preparation are one or more of isomaltose oligosaccharide, xylitol, milk powder, nutritional yeast and fruit powder.

The present invention also provides a preparation method of a microbial preparation, which specifically includes the following steps:

(1) each probiotic is prepared into freeze-dried bacteria powder for subsequent use;

(2) pulverize the traditional Chinese medicine respectively, and then mix to obtain the traditional Chinese medicine mixture;

(3) Mixing the freeze-dried bacteria powder of each probiotic in step (1), then adding it to the traditional Chinese medicine mixture described in step (2), then adding preparation auxiliary components, and fully mixing to obtain a probiotic preparation.

In the method of the present invention, the preparation of each probiotic into freeze-dried bacteria powder refers to any conventional operation method in the art.

Preferably, in the preparation method, in the step (1), a protective agent can also be used in the preparation process of the freeze-dried bacterial powder, and the protective agent can be any protective agent commonly used in the art.

Preferably, the protective agent is composed of the following components in parts by weight: 70-90 parts of 10% skimmed milk powder, 1-15 parts of sucrose, and 0.1-5 parts of glycerol.

Preferably, in the preparation method, in the step (2), the particle size after crushing is not more than 50um.

The beneficial effects of the present invention are:

(1) A kind of composition of the present invention to assist in protecting gastric mucosa and/or relieving stomach distension, the composition is formed by synergistic combination of traditional Chinese medicine and probiotics, the product is preferably three strains of probiotics, which are beneficial to gastric mucosa. It has a good protective effect against injury and/or bloating, and can also regulate the balance of intestinal flora and improve resistance. Through the synergistic combination of traditional Chinese medicine and probiotics of the same source of medicine and food, while regulating and improving immunity, they can promote each other, adjust the body's constitution as a whole, and maintain a normal state of health.

(2) The present invention assists the protection of gastric mucosa and/or the microecological preparation for relieving stomach distension, including traditional Chinese medicine and probiotics with the same origin of medicine and food, and auxiliary components isomaltose oligosaccharide, xylitol, milk powder, nutritional yeast and fruit powder in the product , Prebiotics promote the proliferation of probiotics, while meeting some of the nutritional needs of the body and improving the taste. It has been verified by animal experiments that it has a good protective effect on gastric mucosal damage caused by alcohol and stomach bloating caused by antibiotics. It has been proved by human trials that it has a good effect on relieving stomach pain and bloating, promoting digestion and increasing appetite, etc. It can also adjust The intestinal flora is balanced and the resistance is improved; it is safe, has a long shelf life, and has broad market prospects. The present invention can be any acceptable dosage form in the art, such as powder, tablet, capsule, etc., is convenient, easy to carry, safe, has a long shelf life, and has broad market prospects.

(3) The method for preparing the microecological preparation of the present invention is simple to operate and easy to industrialize production.

Description of drawings

Figure 1. Visual diagram of gastric mucosal injury in mice.

Figure 2. Sections of mouse gastric mucosa.

Figure 3. Visual diagram of gastric distention in mice.

Figure 4. Human body trial service verification feedback results.

Detailed ways

The cited embodiments are used to better illustrate the content of the present invention, but the content of the present invention is not limited to the cited embodiments. Therefore, non-essential improvements and adjustments to the embodiments by those skilled in the art based on the above-mentioned contents of the invention, including any combination of specific embodiments, still belong to the protection scope of the present invention.

Part 1 Application of the composition for assisting in protecting gastric mucosa and/or relieving gastric bloating of the present invention

Example 1 Auxiliary protection of gastric mucosa and/or probiotics for relieving gastric distention

The microecological preparation for assisting the protection of gastric mucosa and/or relieving gastric distention, including traditional Chinese medicine and probiotics of the same origin as medicine and food, is specifically composed of the following components in parts by weight:

25 servings of Hericium erinaceus;

10 pieces of Poria;

21 isomaltooligosaccharides;

17 parts of xylitol;

25 servings of milk powder;

Wherein each gram of the composition is mixed with 2.5×10 ¹⁰ CFU of probiotics; the probiotics are composed of Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045 and Lactobacillus gasseri GS0010, and the Lactobacillus reuteri LY0032 is preserved The number is CCTCC NO: M 2015341, the Lactobacillus plantarum ZW0045 deposit number is CCTCC NO: M 2015127, and the Lactobacillus gasseri GS0010 deposit number is CCTCC NO: M 2015340.

Example 2 Auxiliary protection of gastric mucosa and/or probiotics for relieving gastric distention

The microecological preparation for assisting the protection of gastric mucosa and/or relieving gastric distention, including traditional Chinese medicine and probiotics of the same origin as medicine and food, is specifically composed of the following components in parts by weight:

25 servings of Hericium erinaceus;

10 pieces of Poria;

21 isomaltooligosaccharides;

17 parts of xylitol;

25 servings of milk powder;

Wherein each gram of the composition is mixed with 4.5×10 ⁹ CFU of probiotics; the probiotics are composed of Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045 and Lactobacillus gasseri GS0010, and the Lactobacillus reuteri LY0032 is preserved The number is CCTCC NO: M 2015341, the Lactobacillus plantarum ZW0045 deposit number is CCTCC NO: M 2015127, the Lactobacillus gasseri GS0010 deposit number is CCTCC NO: M 2015340; the Lactobacillus reuteri LY0032, plant milk The ratio of Bacillus ZW0045 and Lactobacillus gasseri GS0010 was 1:1:2.

Example 3 Auxiliary protection of gastric mucosa and/or probiotics for relieving gastric distention

The microecological preparation for assisting the protection of gastric mucosa and/or relieving gastric distention, including traditional Chinese medicine and probiotics of the same origin as medicine and food, is specifically composed of the following components in parts by weight:

25 servings of Hericium erinaceus;

10 pieces of Poria;

15 parts of isomalt oligosaccharide;

Xylitol 12 parts;

20 servings of milk powder;

5 servings of nutritional yeast;

13 servings of fruit powder;

Wherein each gram of the composition is mixed with 6.5×10 ⁹ CFU of probiotics; the probiotics are composed of Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, Lactobacillus gasseri GS0010, Bifidobacterium lactis, Lactobacillus acidophilus The Lactobacillus reuteri LY0032 deposit number is CCTCC NO: M 2015341, the Lactobacillus plantarum ZW0045 deposit number is CCTCC NO: M 2015127, and the Lactobacillus gasseri GS0010 deposit number is CCTCC NO: M 2015340; The viable counts of the Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045 and Lactobacillus gasseri GS0010 account for 80% of the total number of probiotic bacteria, and the rest are supplemented by Bifidobacterium lactis and Lactobacillus acidophilus; The ratio of Lactobacillus yiseri LY0032, Lactobacillus plantarum ZW0045, and Lactobacillus gasseri GS0010 is 2:1:2, and the ratio of Bifidobacterium lactis to Lactobacillus acidophilus is 1:1.

Example 4 Auxiliary protection of gastric mucosa and/or probiotics for relieving gastric distention

The microecological preparation for assisting the protection of gastric mucosa and/or relieving gastric distention, including traditional Chinese medicine and probiotics of the same origin as medicine and food, is specifically composed of the following components in parts by weight:

25 servings of Hericium erinaceus;

10 pieces of Poria;

15 parts of isomalt oligosaccharide;

Xylitol 12 parts;

20 servings of milk powder;

5 servings of nutritional yeast;

13 servings of fruit powder;

Wherein each gram of the composition is mixed with 6.5×10 ⁹ CFU of probiotics; the probiotics are composed of Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, Lactobacillus gasseri GS0010, Bifidobacterium lactis, Lactobacillus acidophilus, Bifidobacterium longum and Lactobacillus rhamnosus; the Lactobacillus reuteri LY0032 preservation number is CCTCC NO: M 2015341, the Lactobacillus plantarum ZW0045 preservation number is CCTCC NO: M 2015127, the Lactobacillus gasseri The preservation number of GS0010 is CCTCCNO: M 2015340; the number of viable bacteria of the Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045 and Lactobacillus gasseri GS0010 accounts for 85% of the total number of probiotic bacteria, and the rest are composed of Bifidobacterium lactis. , Lactobacillus acidophilus, Bifidobacterium longum, Lactobacillus rhamnosus; the ratio of the three bacteria Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, Lactobacillus gasseri GS0010 is 1:1:2, the The ratio of Bifidobacterium lactis, Lactobacillus acidophilus, Bifidobacterium longum, and Lactobacillus rhamnosus is 1:1:1:1.

Embodiment 5 The preparation method of the microecological preparation of auxiliary protection gastric mucosa and/or relieving stomach distension

The preparation method of the present invention is illustrated by taking the probiotic preparation of Example 4 as an example.

Probiotic strains include Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, Lactobacillus gasseri GS0010, Bifidobacterium lactis, Lactobacillus acidophilus, Bifidobacterium longum and Lactobacillus rhamnosus. In the present invention, the conditions for culturing and activating Lactobacillus in probiotics are: 25-40°C for 22-26h, and the conditions for first-class seed culture are: 25-40°C, static culture for 12-24h, fermentation culture The conditions are: under the condition of 25-40 ℃, intermittent shaking culture for 48-96 hours, the stirring speed is intermittent stirring 100r/min, 5min/2h; the culture and activation conditions of bifidobacteria in probiotics are: 25-40 ℃ culture for 22-26 hours , the first-class seed culture conditions are: anaerobic culture at 32-40°C for 20-28h, the seed culture liquid culture conditions are: static culture at 32-40°C for 16-30h, fermentation culture conditions are: 28-40°C, The initial pH value is 6.0-7.0, and the fermentation time is 48-96h.

The cultured and activated medium of Lactobacillus in probiotics is MRS solid medium, and the primary seed medium and fermentation medium are MRS liquid medium without agar; the cultured and activated medium of Bifidobacterium in probiotics is TPY Solid medium, primary seed medium and fermentation medium are all agar-free TPY liquid medium.

The concrete method of this embodiment is as follows:

1. Prepare each probiotic into freeze-dried bacteria powder

(1) Configure the culture medium

The formula of MRS solid medium is: glucose 2.0%, ammonium citrate 0.2%, sodium acetate 0.5%, dipotassium hydrogen phosphate 0.5%, manganese sulfate 0.02%, magnesium sulfate 0.05%, peptone 1.0%, beef extract 1.0%, yeast extract 0.5%, soil temperature -800.1%, 1.5% agar powder (imported), adjust pH to 6.0. Liquid medium is a solid medium formulation without agar.

The formula of TPY solid medium is: casein peptone 1%, soybean peptone 0.5%, yeast powder 0.25%, dipotassium hydrogen phosphate 0.2%, MgCl ₂ 6H2O 0.05%, calcium chloride 0.015%, ZnSO4 7H ₂ O 0.025%, Ferric chloride 0.01%, L-cysteine hydrochloride 0.005%, glucose 1.5%, Tween-80 1mL (1000mL plus 1mL), 1.5% agar powder (import), adjust pH to 6.3-6.6, 115°C Sterilized under conditions for 20 min. Liquid medium is a solid medium formulation without agar.

(2) Cultivation of probiotics

Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, Lactobacillus gasseri GS0010, Lactobacillus acidophilus, and Lactobacillus rhamnosus were respectively inserted into MRS solid slant medium, and cultured at 37°C for 24 hours; then transferred to MRS liquid In the medium, culture at 37°C for 24h to obtain first-class seed liquid; then transfer to fermentation medium with 5% inoculum for high-density culture, and the fermentation condition is intermittent shaking at 37°C for 72h.

Bifidobacterium lactis and Bifidobacterium longum were respectively inserted into TPY solid slant medium, and cultured at 37°C for 24 hours; then transferred to TPY liquid medium, and cultured at 37°C for 24 hours to obtain first-class seed liquid; then Then, 5% of the inoculum was transferred to the fermentation medium for high-density culture, and the fermentation conditions were 37°C for 72 hours.

(3) Preparation of freeze-dried bacteria powder

When the cell density in the fermentation broth reached 10 ⁹ cfu/mL, the fermentation broth was collected. The bacteria were collected by centrifugation, and a protective agent composed of 91% of 10% skimmed milk powder, 8% of sucrose, and 1% of glycerol was added, and freeze-dried into bacteria powder.

2. Pulverize the traditional Chinese medicine respectively and sieve it to obtain the traditional Chinese medicine powder with a particle size of less than 50um, take the weight according to the proportion, and then mix to obtain the traditional Chinese medicine mixture;

3. Take Lactobacillus reuteri LY0032, Lactobacillus plantarum ZW0045, and Lactobacillus gasseri GS0010, mix them according to the ratio of the number of viable bacteria 1:1:2, and add them to the traditional Chinese medicine mixture, then add all the excipients, mix evenly, Lactobacillus yiseri LY0032, Lactobacillus plantarum ZW0045, and Lactobacillus gasseri GS0010 accounted for more than 80% of the total viable bacteria, and then took Bifidobacterium lactis, Lactobacillus acidophilus, Bifidobacterium longum, and mouse Lactobacillus listosus, according to the ratio of viable bacteria 1:1:1:1 to obtain mixed bacteria powder, and mixed bacteria powder with Bifidobacterium lactis, Lactobacillus acidophilus, Bifidobacterium longum, Lactobacillus rhamnosus Make up and mix evenly to obtain a probiotic product, in which the total viable count of probiotics reaches 2.5×10 ¹⁰ cfu/g.

The second part is the research on the synergistic effect of Chinese medicine and probiotics of the composition of the present invention

Example 6 Experimental verification of mouse gastric mucosal injury model induced by ethanol

(1) Experimental process

40 male mice were randomly divided into 4 groups. The 4 groups were named as CK (control group), MX (ethanol-induced mouse gastric mucosal injury model animal), J123 (mixture of LY0032, ZW0045 and GS0010 in equal proportions), J+ Y (micro-ecological preparation, J123+ traditional Chinese medicine), each group of mice was given the corresponding drug by gavage as shown in Table 1, once/d, for 10 days.

Table 1 Animal experiment grouping and operation

Model success judgment indicators:

Morphological observation indicators included the size of stomach, thickness of stomach wall, number of folds, mucosal color and hemorrhage. The gastric mucosa in the normal group was light red, with a smooth surface and a lot of mucus attached. The full-thickness gastric wall was thick with regular folds and elasticity. In the model group, the mucosa was pale, the gastric wall had poor elasticity, the folds were flat or disordered, the mucosa was thinned, and a small amount of mucus was attached, and some gastric mucosa had bleeding spots.

Histopathological examination: mucosal damage and tissue shedding were seen in the model group. The gastric mucosa of the control group was intact, and there was no obvious shedding.

Before the last administration, the mice were fasted for 24 hours, and 1 hour after administration, 0.2 mL of anhydrous ethanol was administered to the rats. The control group was given normal saline instead of anhydrous ethanol. After 3 hours, the mice were weighed and sacrificed by cervical dislocation. The whole stomach was excised, cut along the greater curvature of the stomach, rinsed with normal saline, and the gastric mucosal injury of the mice was observed and fixed with formaldehyde for observation of tissue sections.

(2) Experimental results

The results of gastric mucosal observation in mice are shown in Figure 1. Compared with the control group (CK), the gastric mucosal damage in the model group (MX) was more serious. protective effect is more pronounced. The results of gastric mucosal sections also fully verified this conclusion, as shown in Figure 2. It is indicated that the microecological preparation product of the present invention has a good protective effect on gastric mucosal damage caused by alcohol, that is, the composition of the present invention has a synergistic effect between traditional Chinese medicine and probiotics.

Example 7 Experimental verification of the mouse stomach distention model caused by antibiotics

(1) Experimental process

40 male mice were randomly divided into 4 groups. The 4 groups were named as CK (control group), MX (antibiotic-induced gastric distention model animal), JF (mixture of LY0032, ZW0045 and GS0010 in equal proportions), JY (micro- Ecological product, JF+ Chinese medicine). Mice in each group were given the corresponding drugs by gavage, once/d, for 10 days. The specific groups and operations are shown in Table 2.

Table 2 Animal experiment grouping and operation

Except the control group was given normal saline, each group was given lincomycin hydrochloride by gavage for 1-4 days. From the 5th day, the model group continued to be given lincomycin hydrochloride by gavage. The experimental group was intervened accordingly, and the last administration was 3 hours. After that, the mice were weighed and sacrificed by cervical dislocation. The abdominal cavity was opened to extract the whole stomach, the abdominal cavity was opened to extract the whole stomach, and the gastric distention of the mice was observed.

Model success judgment indicators:

Observations included stomach size and edema. In the normal group, the whole stomach was small, light-colored and elastic. The whole stomach in the model group was larger, darker in color, edematous, and the stomach wall was less elastic and thinner.

(2) Experimental results

After the test, the gastric distention of the mice is shown in Figure 3, and the data of the degree of gastric distention is shown in Table 3. Compared with the model group, both the probiotic group and the product group had a relieving effect on the stomach bloating of mice caused by antibiotics.

Table 3 Stomach bloating degree data

The third part is the research on the application effect of the microecological preparation of the present invention

The application of embodiment 8 microecological preparations (human body trial clothing)

In order to verify the efficacy of the microecological preparation of the present invention in assisting the protection of gastric mucosa and relieving gastric bloating, 10 people with frequent stomach discomfort were screened to participate in the product trial, and 9 feedback forms were received. After a 10-day trial, the feedback showed that the formula has a good effect on relieving stomach pain and bloating, promoting digestion and increasing appetite, and the results are shown in Figure 4.

The micro-ecological preparation of the present invention has been proved to have good effects on relieving stomach pain and bloating, promoting digestion and increasing appetite, etc. in human body trials; resistance, with broad market prospects.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be Modifications or equivalent substitutions without departing from the spirit and scope of the technical solutions of the present invention should be included in the scope of the claims of the present invention.

Claims (9)

1. The composition for assisting in protecting gastric mucosa and/or relieving gastrectasia is formed by synergistically combining medicinal and edible traditional Chinese medicines and probiotics, wherein the medicinal and edible traditional Chinese medicines comprise hericium erinaceus and poria cocos, and the probiotics comprise lactobacillus reuteri (Lactobacillus reuteri) (Poria)Lactobacillus reuteri) Lactobacillus plantarum (II)Lactobacillus Plantarum) And Lactobacillus gasseri: (Lactobacillus gasseri) The medicine and food homologous traditional Chinese medicine is characterized by comprising 20-35 parts of hericium erinaceus and 5-15 parts of poria cocos according to parts by weight, wherein the lactobacillus reuteri is lactobacillus reuteri LY0032, the lactobacillus plantarum is lactobacillus plantarum ZW0045, the lactobacillus gasseri is lactobacillus gasseri GS0010, and the ratio of the three bacteria of the lactobacillus reuteri LY0032, the lactobacillus plantarum ZW0045 and the lactobacillus gasseri GS0010 is 1-2: 1-2: 1-2, the viable count of three bacteria including lactobacillus reuteri LY0032, lactobacillus plantarum ZW0045 and lactobacillus gasseri GS0010 accounts for more than 80% of the total number of probiotics, and the preservation number of the lactobacillus reuteri LY0032 is CCTCC NO: m2015341, wherein the preservation number of the lactobacillus plantarum ZW0045 is CCTCC NO: m2015127, wherein the preservation number of the Lactobacillus gasseri GS0010 is CCTCC NO: m2015340.

2. According to claim1, wherein the probiotic bacteria further comprise bifidobacterium lactis (b), (b)Bifidobacterium animalis) Lactobacillus acidophilus (a)Lactobacillus acidophilus) Bifidobacterium longum (b)Bifidobacterium longum) Lactobacillus rhamnosus (A), (B), (C)Lactobacillus rhamnosus) One or more of (a).

3. Use of a composition according to any one of claims 1-2 for the manufacture of a pharmaceutical formulation for aiding the protection of the gastric mucosa and/or relieving bloating.

4. Use of the composition of any one of claims 1-2 in the preparation of a health product for assisting in protecting gastric mucosa.

5. A microecological formulation for aiding in the protection of the gastric mucosa and/or alleviating bloating, prepared from the composition of any one of claims 1-2 and formulation adjuncts.

6. The probiotic formulation according to claim 5, characterized in that it comprises 10 per gram of probiotic formulation⁹-10¹¹Probiotic bacteria of CFU.

7. The microecological preparation according to claim 5, wherein the auxiliary ingredients of the preparation are one or more of isomaltooligosaccharide, xylitol, milk powder, nutritional yeast and fruit powder.

8. The method of claim 5, comprising the steps of:

(1) preparing each probiotic into freeze-dried bacteria powder for later use;

(2) respectively pulverizing the Chinese medicinal materials, and mixing to obtain Chinese medicinal mixture;

(3) and (3) mixing the freeze-dried powder of each probiotic in the step (1), adding the mixture into the traditional Chinese medicine mixture in the step (2), adding preparation auxiliary components, and fully mixing to obtain the microecological preparation.

9. The preparation method according to claim 8, wherein in the step (1), the protective agent for the freeze-dried mushroom powder comprises the following components in parts by weight: 70-90 parts of 10% skimmed milk powder, 1-15 parts of sucrose and 0.1-5 parts of glycerol.

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