CN112352874A - Anti-piglet diarrhea probiotic preparation and preparation method thereof - Google Patents
Anti-piglet diarrhea probiotic preparation and preparation method thereof Download PDFInfo
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- CN112352874A CN112352874A CN202011255449.4A CN202011255449A CN112352874A CN 112352874 A CN112352874 A CN 112352874A CN 202011255449 A CN202011255449 A CN 202011255449A CN 112352874 A CN112352874 A CN 112352874A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
Abstract
The invention provides a piglet diarrhea resistant probiotic preparation which comprises the following components in parts by weight: 10-15 parts of freeze-dried powder of bacillus subtilis, 4-6 parts of freeze-dried powder of bacillus licheniformis and 1-3 parts of folium artemisiae argyi fermentation freeze-dried powder; the preservation number of the bacillus subtilis is CGMCC No.9660, and the content of viable bacteria in the freeze-dried powder of the bacillus subtilis is more than or equal to 40 hundred million/g; the preservation number of the bacillus licheniformis is CGMCC No.5686, and the viable bacteria content in the freeze-dried powder of the bacillus licheniformis is more than or equal to 50 hundred million/g. The invention also provides a method for preparing the preparation and application of the preparation in preparing medicaments for preventing piglet diarrhea. The piglet diarrhea resistant probiotic preparation can obviously reduce the incidence rate of piglet diarrhea and has good practical application and market prospect.
Description
Technical Field
The invention belongs to the field of biological preparations for preventing and treating piglet diarrhea, and particularly relates to a piglet diarrhea-resistant probiotic preparation and a preparation method thereof.
Background
Because a stable microecosystem is not established in the intestinal tract of the piglet, the piglet has low self resistance, is sensitive to external stimulation and is easily affected by the invasion of various pathogenic microorganisms and various stress factors, so the piglet is easy to have diarrhea symptoms. The diarrhea of piglets is mainly caused by pathogenic microorganisms, parasites, nutritional factors, special physiological functions of piglets, environmental stress factors and the like.
In production practice, in addition to enhancing the feeding management of piglets and sows, the prevention and treatment of diarrhea generally employs antibiotic intervention, such as enrofloxacin soluble powder mixed with water for drinking by piglets. In the prevention and treatment method without adopting antibiotics, Chinese herbal medicines are typically used for prevention and treatment, and for example, a preparation containing Chinese herbal medicine components such as the Chinese pulsatilla root, the coptis root, the golden cypress and the ash bark is used for feeding, so that the effects of clearing heat and cooling blood, eliminating dampness and stopping dysentery, repairing gastrointestinal mucosa, improving the immunity of piglet groups and the like can be achieved.
In addition, with the development of biotechnology, the microbial preparation is used to regulate the balance of the gastrointestinal flora, and people pay more and more attention to the microbial preparation. However, the microbial preparation aiming at piglet diarrhea is still relatively few at present, and the effect is not outstanding.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a probiotic preparation for resisting piglet diarrhea and a preparation method thereof. The piglet diarrhea resistant probiotic preparation can be suitable for various piglet feeds, and the diarrhea incidence rate can be lower than 2%.
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
the probiotic preparation for resisting piglet diarrhea comprises the following components in parts by weight:
10-15 parts of freeze-dried powder of bacillus subtilis, 4-6 parts of freeze-dried powder of bacillus licheniformis and 1-3 parts of folium artemisiae argyi fermentation freeze-dried powder;
the preservation number of the bacillus subtilis is CGMCC No.9660, and the content of viable bacteria in the freeze-dried powder of the bacillus subtilis is more than or equal to 40 hundred million/g; the preservation number of the bacillus licheniformis is CGMCC No.5686, and the content of viable bacteria in the freeze-dried powder of the bacillus licheniformis is more than or equal to 50 hundred million/g;
the folium artemisiae argyi fermentation freeze-dried powder is prepared by the following preparation method:
mixing the ground folium artemisiae argyi powder and the boiled potatoes according to the weight ratio of 3:1, adding sterile water to enable the weight ratio of solid to liquid to be 2.5:1, inoculating 2% of Aspergillus niger YM33182 to ferment, and fermenting at 28-32 ℃ for 36 hours; draining water from the fermented product, and freeze-drying to obtain fructus crataegi fermented dry powder; the Aspergillus niger YM33182 is deposited as follows: CCTCC No. M206H 3.
As a preferred technical scheme of the invention, the piglet diarrhea resistant probiotic preparation comprises the following components in parts by weight: 12 parts of freeze-dried powder of bacillus subtilis, 5 parts of freeze-dried powder of bacillus licheniformis and 2 parts of freeze-dried fermented folium artemisiae argyi powder.
As a preferred embodiment of the present invention, the amount of inoculum is 2% by volume when inoculating Aspergillus niger YM 33182.
As a preferred technical scheme, when the folium artemisiae argyi fermentation dry powder is prepared and fermented, the fermentation is carried out for 36 hours at the temperature of 28-32 ℃; and (4) draining water from the fermented product, and then carrying out freeze drying to obtain the product.
The invention also provides a method for preparing the probiotic preparation for resisting piglet diarrhea, which comprises the following steps: and mixing the freeze-dried powder of the bacillus subtilis, the freeze-dried powder of the bacillus licheniformis and the folium artemisiae argyi fermentation freeze-dried powder to obtain the composition.
The invention also provides application of the anti-piglet diarrhea probiotic preparation in preparation of a medicine for treating piglet diarrhea. When the piglet diarrhea resistant probiotic preparation is specifically applied, the piglet diarrhea resistant probiotic preparation is added into piglet feed according to the weight percentage, and the addition amount is 0.5-0.8%.
The invention has the beneficial effects that:
the piglet diarrhea resistant probiotic preparation can obviously reduce the incidence rate of piglet diarrhea and has good practical application and market prospect.
Detailed Description
The present invention is described in detail below by way of examples, and it should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention.
The bacillus subtilis (with the preservation number of CGMCC No.9660), the bacillus licheniformis (with the preservation number of CGMCC No.5686) and the Aspergillus niger YM33182 (with the preservation number of CCTCC No: M206H3) are all existing strains and are purchased in the market.
In the following examples, the folium artemisiae argyi fermentation freeze-dried powder is obtained by the following preparation method:
mixing the ground folium artemisiae argyi powder and the boiled potatoes according to the weight ratio of 3:1, adding sterile water to enable the weight ratio of solid to liquid to be 2.5:1, inoculating 2% of Aspergillus niger YM33182 to ferment, and fermenting at 28-32 ℃ for 36 hours; draining the fermented product, and freeze-drying to obtain grape seed fermented dry powder; the Aspergillus niger YM33182 is deposited as follows: CCTCC No. M206H 3.
Example 1
1. Experimental animals, namely, weaning Jiangxiang pigs and piglets from a Jiangxiang local farm.
2. The feed additive comprises the following components: lyophilized powder of Bacillus subtilis (with preservation number of CGMCC No.9660) with viable bacteria content of 40 hundred million/g, lyophilized powder of Bacillus licheniformis (with preservation number of CGMCC No.5686) with viable bacteria content of 50 hundred million/g, and folium Artemisiae Argyi fermented lyophilized powder.
3. Diarrhea incidence factor pretest
Basal feed formulation (w/w): 48% of corn, 10% of broken rice, 10% of bran, 10% of bean cake, 8% of fish meal, 2.5% of green material, 0.5% of calcium carbonate, 0.5% of calcium phosphate and 0.5% of salt.
The addition amount of the freeze-dried powder is 0.5 percent (w/w), the freeze-dried powder (the viable bacteria content is more than or equal to 40 hundred million/g) of bacillus subtilis (the preservation number is CGMCC No.9660), the freeze-dried powder (the viable bacteria content is more than or equal to 50 hundred million/g) of bacillus licheniformis (the preservation number is CGMCC No.5686) and the folium artemisiae argyi fermentation freeze-dried powder are respectively added into the basic feed and are respectively marked as an experimental group 1, an experimental group 2 and an experimental group 3.
80 piglets of the Jiangxiang pigs of the experimental animals are taken, the weaning day of the batch of piglets is 12-14 days after birth and breast milk feeding, the batch of piglets is divided into 4 groups, and 20 piglets in each group are respectively fed with basic feed (used as a control group), feed of the experimental group 1, feed of the experimental group 2 and feed of the experimental group 3. The feeding time was 50 days, the feeding was performed twice daily, water was freely drunk, and the incidence of diarrhea was examined, and the results are shown in Table 1. As can be seen from Table 1, the addition of the lyophilized powder of Bacillus subtilis (CGMCC No.9660), Bacillus licheniformis (CGMCC No.5686) or folium Artemisiae Argyi fermented lyophilized powder alone can not effectively reduce the incidence of diarrhea.
TABLE 1
4. Preliminary optimization experiment
The method comprises the steps of combining bacillus subtilis (with the preservation number of CGMCC No.9660) freeze-dried powder, bacillus licheniformis (with the preservation number of CGMCC No.5686) freeze-dried powder and folium artemisiae argyi fermentation freeze-dried powder in different modes to obtain a feed additive, adding the feed additive into a basic feed according to the addition of 0.5% (w/w), inspecting the influence of a mixture on the diarrhea incidence rate of piglets, feeding 20 experimental animals of each experimental group according to a feeding method of diarrhea incidence factor pre-test. The group settings and experimental results are shown in Table 2, Table 2
5. Optimization experiment
According to the pre-optimization experiment, the mixing of the bacillus subtilis (with the preservation number of CGMCC No.9660), the bacillus licheniformis (with the preservation number of CGMCC No.5686) and the folium artemisiae argyi fermentation freeze-dried powder has obvious influence on the incidence rate of piglet diarrhea. After a large number of groping experiments, the feed additive is prepared by adopting the following formula and is added into a basic feed (the addition amount is 0.5 percent (w/w)), and when the number of the experimental animals weaned from the fragrant pig in Yangtze river is 100, the number of diarrhea occurrence is only 1, namely the diarrhea occurrence rate is 1%.
The feed additive amount formula is as follows:
according to the weight portion, 12 portions of freeze-dried powder of bacillus subtilis, 5 portions of freeze-dried powder of bacillus licheniformis and 2 portions of folium artemisiae argyi fermentation freeze-dried powder.
6. Experiment on feed suitability
On the basis of the optimization experiment, the applicability of the feed additive screened by the optimization experiment to different feeds is tested, and the feed formula adopted by the experiment is as follows:
the formula of the feed 1 is as follows: 60 w/w% of rice bran, 30 w/w% of corn flour and 10 w/w% of current season vegetables; the formula of the feed 2 is as follows: 74.86% of corn, 5.5% of wheat bran, 17.5% of soybean meal, 0.8% of stone powder, 0.5% of calcium hydrophosphate, 0.3% of salt, 0.15% of sodium bicarbonate, 0.04% of lysine hydrochloride, 0.08% of choline, and,0.03% of sweetening agent and 0.24% of compound vitamin (consisting of vitamin E, vitamin B2, vitamin K, folic acid, pantothenic acid and nicotinic acid according to the weight ratio of 4:2:1:2:3: 3); the formula of the feed 3 is as follows: 65% of corn, 1.8% of wheat middling, 12.60% of fermented soybean meal, 14.17% of soybean meal, 2.8% of fish meal, 0.28% of oil extraction, 0.91% of stone powder, 1% of calcium hydrophosphate, 0.32% of salt, 0.06% of choline chloride, 0.05% of flavor element, composite premix (containing 100ml/kg of manganese, 1332mg/kg of zinc, 2664mg/kg of iron, 167mg/kg of copper, 5mg/kg of iodine, 5mg/kg of selenium, 57000IU/kg of vitamin A, vitamin D36600IU/kg, vitamin E366 IU/kg, vitamin B2 96mg/kg)1％。
240 weaned Jiangxiang piglets are selected as experimental animals and divided into 6 groups, 40 piglets in each group are fed according to an optimization experiment method, and the diarrhea incidence rate is inspected. Wherein, each feed is provided with a control group (not added with feed additives) and an experimental group (added with feed additives according to 0.5% (w/w)). The experimental results are shown in table 3, and the results show that the additive obtained by the invention can be applied to various feeds, and remarkably reduce the diarrhea incidence rate of piglets.
TABLE 3
Example 2
The formulation of the probiotic formulation (i.e. the feed additive described in example 1) for the adjustment of the anti-piglet diarrhea was as follows:
10 parts of freeze-dried powder of bacillus subtilis, 6 parts of freeze-dried powder of bacillus licheniformis and 1 part of freeze-dried fermented folium artemisiae argyi powder.
100 weaned Jiangxiang piglets are selected as experimental animals and are bred according to the method in the optimization experiment in the example 1, and the result shows that the diarrhea incidence rate is 2%.
Example 3
The formulation of the probiotic formulation (i.e. the feed additive described in example 1) for the adjustment of the anti-piglet diarrhea was as follows:
15 parts of freeze-dried powder of bacillus subtilis, 4 parts of freeze-dried powder of bacillus licheniformis and 3 parts of folium artemisiae argyi fermentation freeze-dried powder.
100 weaned Jiangxiang piglets are selected as experimental animals and are bred according to the method in the optimization experiment in the example 1, and the result shows that the diarrhea incidence rate is 2%.
Claims (7)
1. The piglet diarrhea resistant probiotic preparation is characterized by comprising the following components in parts by weight:
10-15 parts of freeze-dried powder of bacillus subtilis, 4-6 parts of freeze-dried powder of bacillus licheniformis and 1-3 parts of folium artemisiae argyi fermentation freeze-dried powder;
the preservation number of the bacillus subtilis is CGMCC No.9660, and the content of viable bacteria in the freeze-dried powder of the bacillus subtilis is more than or equal to 40 hundred million/g; the preservation number of the bacillus licheniformis is CGMCC No.5686, and the content of viable bacteria in the freeze-dried powder of the bacillus licheniformis is more than or equal to 50 hundred million/g;
the folium artemisiae argyi fermentation freeze-dried powder is prepared by the following preparation method:
mixing the ground folium artemisiae argyi powder and the boiled potatoes according to the weight ratio of 3:1, adding sterile water to enable the weight ratio of solid to liquid to be 2.5:1, inoculating 2% of Aspergillus niger YM33182 to ferment, and fermenting at 28-32 ℃ for 36 hours; draining water from the fermented product, and freeze-drying to obtain fructus crataegi fermented dry powder; the Aspergillus niger YM33182 is deposited as follows: CCTCC No. M206H 3.
2. The probiotic preparation for resisting piglet diarrhea according to claim 1, which is characterized by comprising the following components in parts by weight:
12 parts of freeze-dried powder of bacillus subtilis, 5 parts of freeze-dried powder of bacillus licheniformis and 2 parts of freeze-dried fermented folium artemisiae argyi powder.
3. The probiotic preparation against diarrhea in piglets according to claim 1, characterized in that the amount of inoculation is 2% by volume when inoculated with Aspergillus niger YM 33182.
4. The feeding method according to claim 1, wherein the fermentation is carried out at 28 to 32 ℃ for 36 hours when the fermented dry powder of mugwort leaves is prepared; and (4) draining water from the fermented product, and then carrying out freeze drying to obtain the product.
5. The method for preparing the probiotic preparation for resisting piglet diarrhea according to claim 1, wherein the lyophilized powder of bacillus subtilis, the lyophilized powder of bacillus licheniformis and the folium artemisiae argyi fermented lyophilized powder are mixed to obtain the probiotic preparation.
6. The use of the probiotic preparation for treating piglet diarrhea according to any one of claims 1 to 4 in the preparation of a medicament for treating piglet diarrhea.
7. The application of the probiotic preparation for resisting piglet diarrhea according to claim 6, wherein the probiotic preparation for resisting piglet diarrhea is added into piglet feed in an amount of 0.5-0.8% in percentage by weight.
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Cited By (3)
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| CN112715763A (en) * | 2020-11-11 | 2021-04-30 | 贵州省畜牧兽医研究所 | Guizhou local pig breeding method with high intramuscular fat content |
| CN114191531A (en) * | 2021-10-16 | 2022-03-18 | 四川辉氏生物技术有限公司 | Artemisia argyi fermentation product for cattle and sheep diarrhea and preparation method thereof |
| CN115216426A (en) * | 2022-06-27 | 2022-10-21 | 河南科技学院 | Bacillus amyloliquefaciens LL-2 and application thereof |
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