CN101999556A - Special animal nutrient for beef cattle and preparation method thereof - Google Patents
Special animal nutrient for beef cattle and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a special animal nutrient for beef cattle and a preparation method thereof. The special animal nutrient for beef cattle comprises the following components in percentage by weight: 35-55% of fulvic acid, 0.1-0.3% of total flavone from dandelion and 44.7-64.7% of bitter bean meal fermented culture. The preparation method of the special animal nutrient for beef cattle comprises the following steps: extracting the fulvic acid; preparing the bitter bean meal fermented culture; and uniformly mixing 35-55 wt% of fulvic acid, 0.1-0.3 wt% of total flavone from dandelion and 44.7-64.7 wt% of bitter bean meal fermented culture. The special animal nutrient for beef cattle has the obvious effects of improving intestine and stomach functions, enhancing body immunity, lowering incidence of rumen diseases, increasing weight and improving beef quality. Besides, the special animal nutrient for beef cattle is safe and convenient, has stable performance and is easy to store.
Description
Technical field
The present invention relates to a kind of animal nutrient and preparation method thereof, particularly relate to a kind of beef cattle tailored version animal nutrient and preparation method thereof.
Background technology
Supply falls short of demand always for beef cattle, and it is over half that developed regions, world beef accounts for the meat ratio, and China only accounts for about 10%. the beef occupancy volume per person, world developed country is more than 50 kilograms, 10 kilograms per capita in the world, and 5 kilograms of China's less thaies, particularly some regional less thaies of south are 2 kilograms.And in beef cattle is raised; the cud disease is having a strong impact on the development of beef cattle aquaculture always; beef cattle suffers from cud after being ill; can occur normal digestion of cud and motion function disorderly or breathe be the devil, illness such as the one-sided or rapid expansion of bilateral abdominal circumference; as untimely, correctly treat, not only can have a strong impact on growing of beef cattle, also can influence the meat of beef cattle; even cause the death of beef cattle, thereby cause enormous economic loss for the beef cattle raiser.
Since the forties in last century, antibiotic came out, antibiotic is widely used in each link of aquaculture, particularly in beef cattle was cultured, antibiotic can be used as the composition of feed addictive, the drug ingedient of treatment disease etc. and plays effects such as health care, treatment and growth promotion.But in the antibiotic large-scale application, also there is following major defect in it:
(1) resistance to the action of a drug is serious.A large amount of irrational use antibiotic not only cause antibody-resistant bacterium quantity to increase, but also multiple drug resistance strain occurred.
(2) immunity degradation.A large amount of antibiotic are ingested behind the body, can be distributed to each histoorgan such as lymph node, kidney,liver,spleen, thymus gland, lung and bone with blood circulation, antibiotic can cause antigenic quality to reduce, directly influence immunologic process, thereby the inoculation to vaccine produces harmful effect, and the immunocompetence of animal body also can be weakened gradually.
(3) autogenous infection and suprainfection.Antibiotic is difficult to avoid can influence in the body profitable strain growth simultaneously acting on pathogen, especially long-term a large amount of antibiotic that uses can cause flora imbalance in the body, and microecological balance destroys, the harmful bacterium that hides in vivo takes advantage of the occasion to breed in a large number, and causes endogenous infection; Antibiotic can be eliminated sensitive bacteria in the body, causes a large amount of rooms on the certain micro-organisms attachment point in vivo, and taking advantage of a weak point for extraneous resistance germ offers an opportunity, thereby causes external source to infect.Suprainfection also is owing to when using a large amount of antibiotic to kill certain bacterium, destroy microecological balance, and another or multiple endogenous or external source germ infect once more immediately that body causes.
(4) jeopardize human health.Antibiotic is distributed to almost each organ of whole body after being absorbed in the body, and most antibiotics is excreted by ight soil, the part antibiotic residue is also arranged in vivo.After being excreted in the environment, the antibiotic of some stable in properties still can have a very long time, thereby cause the medicament residue in the environment, these remaining medicines are slowly accumulated in human body and other plant body by livestock products and environment, finally come together in human body with various approach, cause human body to produce a large amount of antibody-resistant bacterium, lose resistance, or body is produced toxic action because of accumulating in a large number to some disease.
Summary of the invention
The purpose of this invention is to provide a kind of beef cattle tailored version animal nutrient and preparation method thereof, overcome effectively that the resistance to the action of a drug that a large amount of unreasonable use antibiotic of prior art cause is serious, immunity degradation, autogenous infection and suprainfection, the technological deficiency such as human health that jeopardizes.
For achieving the above object, the invention provides a kind of beef cattle tailored version animal nutrient, comprise the constituent of following percentage by weight: fulvic acid 35%-55%, dandelion general flavone 0.1%-0.3% and bitter bean pulp fermentation culture 44.7%-64.7%.
On the basis of technique scheme, described bitter bean pulp fermentation culture comprises: bitter dregs of beans substrate, S. cervisiae, bacillus subtilis and bacillus licheniformis.
Further, described bitter dregs of beans substrate comprises the constituent of following percentage by weight: bitter dregs of beans 90%-97%, corn flour 2%-8% and beet molasses 0.5%-2%.
Preferably, the number of viable of S. cervisiae is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu, the number of viable summation of bacillus subtilis and described bacillus licheniformis is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu.
The present invention also provides a kind of preparation method of beef cattle tailored version animal nutrient, comprising:
Extract fulvic acid;
Prepare bitter bean pulp fermentation culture;
With percentage by weight is that the described bitter bean pulp fermentation culture that the described fulvic acid of 35%-55%, dandelion general flavone that percentage by weight is 0.1%-0.3% and percentage by weight are 44.7%-64.7% mixes.
On the basis of technique scheme, described extraction fulvic acid specifically comprises:
Fulvic acid is extracted raw material pulverizing to the 60-80 order, and the fulvic acid after pulverizing is extracted raw material by 1: it is in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, extracts 2-3 hour down for 60 ℃-80 ℃ in temperature;
Said extracted liquid is removed the gred the back by 100: the part by weight adding mass concentration of 1-2 is 30% hydrogen peroxide, activates 1.5-2 hour down for 70 ℃-80 ℃ in temperature, obtains activating solution;
With molar concentration be the hydrochloric acid solution of 1mol/L and mass concentration be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants, with above-mentioned activating solution and described compound precipitants by 1: the volume ratio of 50-80 mixes, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid.
It is brown coal, weathered coal or peat that described fulvic acid extracts raw material.
Further, the bitter bean pulp fermentation culture of described preparation is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled, mix afterwards and descended aerobic fermentation 16-36 hour more first anaerobic fermentation 38-60 hour for 20 ℃-40 ℃ in temperature.
Preferably, described bitter dregs of beans substrate comprises the constituent of following percentage by weight: bitter dregs of beans 90-97%, corn flour 2-8%, beet molasses 0.5-2%.Described composite microbial inoculant liquid comprises: S. cervisiae, bacillus subtilis and bacillus licheniformis, the number of viable of S. cervisiae is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu, the number of viable summation of bacillus subtilis and described bacillus licheniformis is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu.
The invention provides a kind of beef cattle tailored version animal nutrient and preparation method thereof, have the following advantages:
1, improves functions of intestines and stomach, enhancing immunity of organisms.After fulvic acid enters alimentary canal in the beef cattle tailored version animal nutrient of the present invention, can stimulate the coat of the stomach mucous membrane to increase gastric secretion, increase appetite; Because fulvic acid has surface-active, can promote nutrients to enter body sooner again, improve absorptivity and conversion ratio; Can also stimulate beneficial microorganism growth in the beef cattle stomach and intestine, suppress the breeding of spoilage organisms, prevent the poisoning of beef cattle hydrochloric acid in gastric juice; Fulvic acid has stronger absorption and complexing power, can effectively adsorb poisonous and harmful substance and bacteriotoxin in the stomach, has detoxifcation, alleviates excreta stink and significantly astringing to arrest diarrhea effect, helps the improvement of stable breeding drylot feeding environment.Simultaneously, fulvic acid and dandelion general flavone can promote the activity of multiple probiotic (S. cervisiae, bacillus subtilis, bacillus licheniformis etc.) propagation and enhancing plurality of enzymes (polyphenol oxidase, peroxidase, ascorbic acid oxidase etc.) in the beef cattle tailored version animal nutrient of the present invention, strengthen immunity of organism competent cell and body immune system rejection ability, body immunity is improved.
2, reduce the sick incidence of disease of cud, put on weight, improve beef quality.Fulvic acid and dandelion general flavone can promote multiple probiotic (S. cervisiae, bacillus subtilis, bacillus licheniformis etc.) propagation in the beef cattle tailored version animal nutrient of the present invention, satisfy the activity of microorganism in the cud, promote cud physiogeny, strengthen the beef cattle adaptive capacity of environment to external world, cud disease to beef cattle has good prevention effect, simultaneously can also increase beef cattle weight and significantly improve beef quality, make fine and tender taste, meat flavour deliciousness.
3, safe ready.Beef cattle tailored version animal nutrient of the present invention only need be admixed in proportion to feed in the feed and get final product, and is simple and convenient; Simultaneously, beef cattle tailored version animal nutrient of the present invention does not contain antibiotic and/or antibiotics material, and the raiser can relievedly use beef cattle, can slowly not accumulate in human body by livestock products and environment, and the mankind are caused healthy hidden danger.
4, stable performance, easily preservation.The shortcoming of probiotic (S. cervisiae, bacillus subtilis, bacillus licheniformis etc.) is a poor heat stability in the beef cattle tailored version animal nutrient of the present invention, is in modified, granulation, heat treatment such as expanded or long-term storage all causes inactivation easily under normal temperature condition; And active ingredients such as fulvic acid, dandelion general flavone have also been added in the beef cattle tailored version animal nutrient of the present invention simultaneously, become the composite parts product, this compound product can remedy each composition deficiency to each other in the process of processing, preservation, use, make the effect maximization of beef cattle tailored version animal nutrient of the present invention.
Below by embodiment, technical scheme of the present invention is described in further detail.
Description of drawings
Fig. 1 is preparation method's flow chart of beef cattle tailored version animal nutrient of the present invention;
Fig. 2 extracts the flow chart of fulvic acid for the present invention.
The specific embodiment
Further specify the technical scheme of beef cattle tailored version animal nutrient of the present invention below by specific embodiment.
Beef cattle tailored version animal nutrient first embodiment of the present invention
Beef cattle tailored version animal nutrient comprises the constituent of following percentage by weight: fulvic acid 35%, dandelion general flavone 0.3% and bitter bean pulp fermentation culture 64.7%.Described bitter bean pulp fermentation culture comprises: percentage by weight is that 90% bitter dregs of beans, percentage by weight are that 8% corn flour, percentage by weight are 2% beet molasses, and the number of viable of S. cervisiae is 1 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu, the number of viable summation of bacillus subtilis and bacillus licheniformis is 10 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu.
Beef cattle tailored version animal nutrient second embodiment of the present invention
Beef cattle tailored version animal nutrient comprises the constituent of following percentage by weight: fulvic acid 45%, dandelion general flavone 0.2% and bitter bean pulp fermentation culture 54.8%.Described bitter bean pulp fermentation culture comprises: percentage by weight is that 95% bitter dregs of beans, percentage by weight are that 4% corn flour, percentage by weight are 1% beet molasses, and the number of viable of S. cervisiae is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu, the number of viable summation of bacillus subtilis and bacillus licheniformis is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu.
Beef cattle tailored version animal nutrient the 3rd embodiment of the present invention
Beef cattle tailored version animal nutrient comprises the constituent of following percentage by weight: fulvic acid 55%, dandelion general flavone 0.1% and bitter bean pulp fermentation culture 44.9%.Described bitter bean pulp fermentation culture comprises: percentage by weight is that 97% bitter dregs of beans, percentage by weight are that 2% corn flour, percentage by weight are 1% beet molasses, and the number of viable of S. cervisiae is 10 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu, the number of viable summation of bacillus subtilis and bacillus licheniformis is 1 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu.
Beef cattle tailored version animal nutrient the 4th embodiment of the present invention
Beef cattle tailored version animal nutrient comprises the constituent of following percentage by weight: fulvic acid 55%, dandelion general flavone 0.3% and bitter bean pulp fermentation culture 44.7%.Described bitter bean pulp fermentation culture comprises: percentage by weight is that 95% bitter dregs of beans, percentage by weight are that 4.5% corn flour, percentage by weight are 0.5% beet molasses, and the number of viable of S. cervisiae is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu, the number of viable summation of bacillus subtilis and bacillus licheniformis is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu.
In the technical scheme of the invention described above beef cattle tailored version animal nutrient first embodiment to the four embodiment, described bitter dregs of beans is the residue of Sophora alopecuroide after extracting matrine, available from Inner Mongol Huimin biotech firm.
Beef cattle tailored version animal nutrient of the present invention can be admixed the beef cattle of feeding in the feed according to the ratio of 5-15 kg/ton and get final product.
Technical scheme by the invention described above beef cattle tailored version animal nutrient first embodiment to the four embodiment as can be seen, compared with prior art, beef cattle tailored version animal nutrient of the present invention have improve functions of intestines and stomach, strengthen immunity of organisms, reduce the sick incidence of disease of cud, put on weight, improve beef quality, safe ready, stable performance, the remarkable result easily preserved.
Fig. 1 is preparation method's flow chart of beef cattle tailored version animal nutrient of the present invention, is specially:
Fig. 2 extracts the flow chart of fulvic acid for the present invention, and in technical scheme shown in Figure 1, step 1 is specially:
Step 11, fulvic acid is extracted raw material pulverizing to the 60-80 order, the fulvic acid after pulverizing is extracted raw material by 1: it is in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, in extraction 2-3 hour down of 60 ℃-80 ℃ of temperature;
Step 12, with said extracted liquid slagging-off back by 100: it is 30% hydrogen peroxide that the part by weight of 1-2 adds mass concentration, activates 1.5-2 hour down for 70 ℃-80 ℃ in temperature, obtains activating solution;
Step 13, with molar concentration be the hydrochloric acid solution of 1mol/L and mass concentration be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants, with above-mentioned activating solution and described compound precipitants by 1: the volume ratio of 50-80 mixes, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid.
On the basis of technique scheme, it is brown coal, weathered coal or peat that described fulvic acid extracts raw material.Further, the bitter bean pulp fermentation culture of described preparation is specially: with composite microbial inoculant liquid in 3-5: 6 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled, mix afterwards and descended aerobic fermentation 16-36 hour more first anaerobic fermentation 38-60 hour for 20 ℃-40 ℃ in temperature.Preferably, described bitter dregs of beans substrate comprises the constituent of following percentage by weight: bitter dregs of beans 90-97%, corn flour 2-8%, beet molasses 0.5-2%.Described composite microbial inoculant liquid comprises: S. cervisiae, bacillus subtilis and bacillus licheniformis, the number of viable of S. cervisiae is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu, the number of viable summation of bacillus subtilis and described bacillus licheniformis is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu.
Preparation method first embodiment of beef cattle tailored version animal nutrient of the present invention
Step 101, brown coal powder is broken to 70 orders, it is in 4% the sodium hydroxide solution that the brown coal after pulverizing are added mass concentration by 1: 8 part by weight, in the extraction 2.5 hours down of 70 ℃ of temperature, obtains extract;
Step 102, said extracted liquid is added mass concentration through centrifugation slagging-off back by 100: 1.5 part by weight is 30% hydrogen peroxide, activates 2 hours down for 75 ℃ in temperature, obtains activating solution;
Step 103, be that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 1 volume ratio for the hydrochloric acid solution of 1mol/L and mass concentration with molar concentration, above-mentioned activating solution is mixed with the volume ratio of described compound precipitants by 1: 50, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid of PH=6.5;
Step 104, bitter dregs of beans substrate and water are become reconciled by 2: 1 weight ratio, again composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 1: 5 ml/kg ratio, mix afterwards and descended first anaerobic fermentations 60 hours for 20 ℃ in temperature, aerobic fermentation is 16 hours again, obtain bitter bean pulp fermentation culture, number of viable is 1 * 10 in the bitter bean pulp fermentation culture of every gram
8The number of viable summation is 10 * 10 in the S. cervisiae of cfu, the bitter bean pulp fermentation culture of every gram
8The bacillus subtilis of cfu and bacillus licheniformis, wherein, described bitter dregs of beans substrate comprises: percentage by weight is that 90% bitter dregs of beans, percentage by weight are that 8% corn flour, percentage by weight are 2% beet molasses, and described composite microbial inoculant liquid comprises: number of viable is 1 * 10 in every milliliter of composite microbial inoculant liquid
10The number of viable summation is 10 * 10 in the S. cervisiae of cfu, the every milliliter of composite microbial inoculant liquid
10The bacillus subtilis of cfu and bacillus licheniformis;
Step 105, be that 35% described feed grade fulvic acid, percentage by weight are that 0.3% dandelion general flavone and percentage by weight are that 64.7% described bitter bean pulp fermentation culture mixes with percentage by weight.
Preparation method second embodiment of beef cattle tailored version animal nutrient of the present invention
Step 201, weathered coal is crushed to 60 orders, it is in 3% the sodium hydroxide solution that the weathered coal after pulverizing is added mass concentration by 1: 10 part by weight, in the extraction 2 hours down of 60 ℃ of temperature, obtains extract;
Step 202, said extracted liquid is added mass concentration through centrifugation slagging-off back by 100: 1 part by weight is 30% hydrogen peroxide, activates 1.5 hours down for 70 ℃ in temperature, obtains activating solution;
Step 203, be that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 2 volume ratio for the hydrochloric acid solution of 1mol/L and mass concentration with molar concentration, above-mentioned activating solution is mixed with the volume ratio of described compound precipitants by 1: 70, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid of PH=6.8;
Step 204, bitter dregs of beans substrate and water are become reconciled by 2: 1 weight ratio, composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 1: 8 ml/kg ratio, mix afterwards and descended first anaerobic fermentations 48 hours for 25 ℃ in temperature, aerobic fermentation is 24 hours again, obtain bitter bean pulp fermentation culture, number of viable is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8The number of viable summation is 5 * 10 in the S. cervisiae of cfu, the bitter bean pulp fermentation culture of every gram
8The bacillus subtilis of cfu and bacillus licheniformis, wherein, described bitter dregs of beans substrate comprises: percentage by weight is that 95% bitter dregs of beans, percentage by weight are that 4% corn flour, percentage by weight are 1% beet molasses, and described composite microbial inoculant liquid comprises: number of viable is 5 * 10 in every milliliter of composite microbial inoculant liquid
10The number of viable summation is 5 * 10 in the S. cervisiae of cfu, the every milliliter of composite microbial inoculant liquid
10The bacillus subtilis of cfu and bacillus licheniformis;
Step 205, be that 45% described feed grade fulvic acid, percentage by weight are that 0.2% dandelion general flavone and percentage by weight are that 54.8% described bitter bean pulp fermentation culture mixes with percentage by weight.
Preparation method the 3rd embodiment of beef cattle tailored version animal nutrient of the present invention
Step 301, peat dust is broken to 80 orders, it is in 2% the sodium hydroxide solution that the peat after pulverizing is added mass concentration by 1: 9 part by weight, in the extraction 3 hours down of 80 ℃ of temperature, obtains extract;
Step 302, said extracted liquid is added mass concentration through centrifugation slagging-off back by 100: 2 part by weight is 30% hydrogen peroxide, activates 1.8 hours down for 80 ℃ in temperature, obtains activating solution;
Step 303, be that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 3 volume ratio for the hydrochloric acid solution of 1mol/L and mass concentration with molar concentration, above-mentioned activating solution is mixed with the volume ratio of described compound precipitants by 1: 80, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid of PH=7;
Step 304, bitter dregs of beans substrate and water are become reconciled by 2: 1 weight ratio, composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 1: 10 ml/kg ratio, mix afterwards and descended first anaerobic fermentations 38 hours for 40 ℃ in temperature, aerobic fermentation is 36 hours again, obtain bitter bean pulp fermentation culture, number of viable is 10 * 10 in the bitter bean pulp fermentation culture of every gram
8The number of viable summation is 1 * 10 in the S. cervisiae of cfu, the bitter bean pulp fermentation culture of every gram
8The bacillus subtilis of cfu and bacillus licheniformis, wherein, described bitter bean pulp fermentation culture comprises: percentage by weight is that 97% bitter dregs of beans, percentage by weight are that 2% corn flour, percentage by weight are 1% beet molasses, and described composite microbial inoculant liquid comprises: number of viable is 10 * 10 in every milliliter of composite microbial inoculant liquid
10The number of viable summation is 1 * 10 in the S. cervisiae of cfu, the every milliliter of composite microbial inoculant liquid
10The bacillus subtilis of cfu and bacillus licheniformis;
Step 305, be that 55% described feed grade fulvic acid, percentage by weight are that 0.1% dandelion general flavone and percentage by weight are that 44.9% described bitter bean pulp fermentation culture mixes with percentage by weight.
Preparation method the 4th embodiment of beef cattle tailored version animal nutrient of the present invention
Step 401, weathered coal is crushed to 60 orders, it is in 3% the sodium hydroxide solution that the weathered coal after pulverizing is added mass concentration by 1: 10 part by weight, in the extraction 2 hours down of 60 ℃ of temperature, obtains extract;
Step 402, said extracted liquid is added mass concentration through centrifugation slagging-off back by 100: 1 part by weight is 30% hydrogen peroxide, activates 1.5 hours down for 70 ℃ in temperature, obtains activating solution;
Step 403, be that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 2 volume ratio for the hydrochloric acid solution of 1mol/L and mass concentration with molar concentration, above-mentioned activating solution is mixed with the volume ratio of described compound precipitants by 1: 70, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid of PH=6.8;
Step 404, bitter dregs of beans substrate and water are become reconciled by 2: 1 weight ratio, composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 1: 8 ml/kg ratio, mix afterwards and descended first anaerobic fermentations 48 hours for 25 ℃ in temperature, aerobic fermentation is 24 hours again, obtain bitter bean pulp fermentation culture, number of viable is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8The number of viable summation is 5 * 10 in the S. cervisiae of cfu, the bitter bean pulp fermentation culture of every gram
8The bacillus subtilis of cfu and bacillus licheniformis, wherein, described bitter dregs of beans substrate comprises: percentage by weight is that 95% bitter dregs of beans, percentage by weight are that 4.5% corn flour, percentage by weight are 0.5% beet molasses, and described composite microbial inoculant liquid comprises: number of viable is 5 * 10 in every milliliter of composite microbial inoculant liquid
10The number of viable summation is 5 * 10 in the S. cervisiae of cfu, the every milliliter of composite microbial inoculant liquid
10The bacillus subtilis of cfu and bacillus licheniformis;
Step 405, be that 55% described feed grade fulvic acid, percentage by weight are that 0.3% dandelion general flavone and percentage by weight are that 44.7% described bitter bean pulp fermentation culture mixes with percentage by weight.
In the technical scheme of preparation method first embodiment to the of the invention described above beef cattle tailored version animal nutrient four embodiment, described bitter dregs of beans is the residue of Sophora alopecuroide after extracting matrine, available from Inner Mongol Huimin biotech firm.
Can admix the beef cattle of feeding in the feed according to the ratio of 5-15 kg/ton by the beef cattle tailored version animal nutrient of the preparation method of the invention described above beef cattle tailored version animal nutrient preparation gets final product.
By the technical scheme of preparation method first embodiment to the four embodiment of the invention described above beef cattle tailored version animal nutrient as can be seen, compared with prior art, by the beef cattle tailored version animal nutrient of the preparation method of beef cattle tailored version animal nutrient of the present invention preparation have improve functions of intestines and stomach, strengthen immunity of organisms, reduce the sick incidence of disease of cud, put on weight, improve beef quality, safe ready, stable performance, the remarkable result easily preserved.
The remarkable efficacy of beef cattle tailored version animal nutrient of the present invention is described below by concrete test.
180 ages in days, healthy 36 anosis hybrid beef cattles are selected in test for use, set up control group and test group separately, 18 hybrid beef cattles of every processed group, with reference to beef cattle feeding standard (China, 2004) preparation basal diet, examination ox with the basal diet of feeding is a control group, beef cattle tailored version animal nutrient of the present invention is added into according to the ratio of 10 kg/ton constitute the test daily ration in the basal diet, and be test group with the examination ox of feeding experiment daily ration.Experimental period is 60 days.
Result of the test shows:
1, a day nutrient digestibility significantly improves.In whole test in the phase, test group has improved 12.55% than average day crude protein digestibility of control group, test group has improved 31.11% than average day dry digestibility of control group, and test group has improved 14.86% than average day crude fiber digestibility of control group.
2, the daily gain rate significantly improves.In phase, test group has improved 29.66% than the average daily gain rate of control group in whole test.
In addition, a large amount of experimental datas prove, beef cattle is edible for a long time add the feed of beef cattle tailored version animal nutrient of the present invention after, the incidence of disease of cud disease significantly reduces, and the beef fine and tender taste after butchering, meat flavour deliciousness.
Beef cattle tailored version animal nutrient of the present invention have improve functions of intestines and stomach, strengthen immunity of organisms, reduce the sick incidence of disease of cud, put on weight, improve beef quality, safe ready, stable performance, the remarkable result easily preserved.
It should be noted last that, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not break away from the spirit and scope of technical solution of the present invention.
Claims (10)
1. a beef cattle tailored version animal nutrient is characterized in that, comprises the constituent of following percentage by weight: fulvic acid 35%-55%, dandelion general flavone 0.1%-0.3% and bitter bean pulp fermentation culture 44.7%-64.7%.
2. beef cattle tailored version animal nutrient according to claim 1 is characterized in that, described bitter bean pulp fermentation culture comprises: bitter dregs of beans substrate, S. cervisiae, bacillus subtilis and bacillus licheniformis.
3. beef cattle tailored version animal nutrient according to claim 2 is characterized in that, described bitter dregs of beans substrate comprises the constituent of following percentage by weight: bitter dregs of beans 90%-97%, corn flour 2%-8% and beet molasses 0.5%-2%.
4. beef cattle tailored version animal nutrient according to claim 2 is characterized in that, the number of viable of S. cervisiae is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
810 * 10
8Cfu, the number of viable summation of bacillus subtilis and described bacillus licheniformis is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu.
5. a preparation method who prepares the described beef cattle tailored version of arbitrary claim animal nutrient among the claim 1-4 is characterized in that, comprising:
Extract fulvic acid;
Prepare bitter bean pulp fermentation culture;
With percentage by weight is that the described bitter bean pulp fermentation culture that the described fulvic acid of 35%-55%, dandelion general flavone that percentage by weight is 0.1%-0.3% and percentage by weight are 44.7%-64.7% mixes.
6. the preparation method of beef cattle tailored version animal nutrient according to claim 5 is characterized in that, described extraction fulvic acid specifically comprises:
Fulvic acid is extracted raw material pulverizing to the 60-80 order, and the fulvic acid after pulverizing is extracted raw material by 1: it is in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, extracts 2-3 hour down for 60 ℃-80 ℃ in temperature;
Said extracted liquid is removed the gred the back by 100: the part by weight adding mass concentration of 1-2 is 30% hydrogen peroxide, activates 1.5-2 hour down for 70 ℃-80 ℃ in temperature, obtains activating solution;
With molar concentration be the hydrochloric acid solution of 1mol/L and mass concentration be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants, with above-mentioned activating solution and described compound precipitants by 1: the volume ratio of 50-80 mixes, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid.
7. the preparation method of beef cattle tailored version animal nutrient according to claim 6 is characterized in that, it is brown coal, weathered coal or peat that described fulvic acid extracts raw material.
8. the preparation method of beef cattle tailored version animal nutrient according to claim 5, it is characterized in that, the bitter bean pulp fermentation culture of described preparation is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled, mix afterwards and descended aerobic fermentation 16-36 hour more first anaerobic fermentation 38-60 hour for 20 ℃-40 ℃ in temperature.
9. the preparation method of beef cattle tailored version animal nutrient according to claim 8 is characterized in that, described bitter dregs of beans substrate comprises the constituent of following percentage by weight: bitter dregs of beans 90-97%, corn flour 2-8%, beet molasses 0.5-2%.
10. the preparation method of beef cattle tailored version animal nutrient according to claim 8, it is characterized in that, described composite microbial inoculant liquid comprises: S. cervisiae, bacillus subtilis and bacillus licheniformis, the number of viable of S. cervisiae is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu, the number of viable summation of bacillus subtilis and described bacillus licheniformis is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu.
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| CN108391748A (en) * | 2018-02-07 | 2018-08-14 | 于长青 | A kind of liquid feed additive and preparation method thereof improving poultry intestinal health |
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