CN101999555B - Animal nutrient special for sheep and method for preparing the same - Google Patents
Animal nutrient special for sheep and method for preparing the same Download PDFInfo
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Abstract
The invention relates to animal nutrient special for sheep and a method for preparing the same. The animal nutrient special for the sheep comprises the following components in percentage by weight: 40 to 60 percent of fulvic acid, 0.1 to 0.3 percent of dandelion general flavone and 39.7 to 59.7 percent of bitter bean pulp fermentation culture. The method for preparing the animal nutrient special for the sheep comprises the following steps of: extracting fulvic acid; preparing the bitter bean pulp fermentation culture; and uniformly mixing 40 to 60 weight percent of fulvic acid, 0.1 to 0.3 weight percent of dandelion general flavone and 39.7 to 59.7 weight percent of bitter bean pulp fermentation culture. The animal nutrient special for the sheep has obvious advantages of improving stomach function, enhancing immunity of an organism, reducing morbidity of gastrointestinal disorders, increasing weight and improving mutton quality, along with safety and convenience, and stable performance and is easy to store.
Description
Technical field
The present invention relates to a kind of animal nutrient and preparation method thereof, particularly relate to a kind of sheep tailored version animal nutrient and preparation method thereof.
Background technology
Sheep is raised the history in existing more than 5000 in China.Although the amount of livestock on hand of China sheep comes out at the top in worldwide, but still need a large amount of import mutton and the sheep that lives every year, it is mainly owing to the sheep husbandry of China, the sheep husbandry in pastoral area particularly, main still by a day sheep raising, the volume increase of mutton mainly realizes by increasing the number of animals raised, and the mutton great majority of supplying on China mutton market also are old, a little less than, residual superseded sheep, the lamb of high-quality high-grade is very few, therefore, extensive management, feed nutrition is unbalanced, feed conversion rate is low, the problems such as quality of mutton is difficult to control have seriously hindered the sound development of China's sheep husbandry.In addition, gastroenteritis, lamb dysentery, blue tongue disease are diseases more common in the sheep feeding process, as untimely, correctly prevent and treat, the normal growth that not only can have a strong impact on sheep is grown, also can affect the meat of sheep, even cause sheep dead, cause huge economic loss for the sheep raiser.
Since the forties in last century, antibiotic came out, antibiotic is widely used in the links of aquaculture, particularly in the sheep cultivation, antibiotic can be used as the composition of feed addictive, the drug ingedient for the treatment of disease etc. and plays the effects such as health care, treatment and growth promotion.But in the antibiotic large-scale application, also there is following major defect in it:
(1) resistance to the action of a drug is serious.A large amount of irrational use antibiotic not only cause antibody-resistant bacterium quantity to increase, but also multiple drug resistance strain occurred.
(2) immunity degradation.A large amount of antibiotic are ingested behind the body, can be distributed to blood circulation each histoorgan such as lymph node, kidney,liver,spleen, thymus gland, lung and bone, antibiotic can cause antigenic quality to reduce, directly affect immunologic process, thereby the inoculation to vaccine produces harmful effect, and the immunocompetence of animal body also can be weakened gradually.
(3) autogenous infection and suprainfection.Antibiotic is difficult to avoid can affect simultaneously in the body profitable strain growth acting on pathogen, especially long-term a large amount of antibiotic that uses can cause flora imbalance in the body, and microecological balance destroys, in vivo the harmful bacteria of the hiding amount reproduction of taking advantage of the occasion, and cause endogenous infection; Antibiotic can be eliminated sensitive bacteria in the body, causes a large amount of rooms on the certain micro-organisms attachment point in vivo, and taking advantage of a weak point for extraneous resistance germ offers an opportunity, thereby causes external source to infect.Suprainfection also is owing to when using a large amount of antibiotic to kill certain bacterium, destroy microecological balance, and another or multiple endogenous or external source germ immediately reinfect body are caused.
(4) jeopardize human health.Antibiotic is distributed to almost each organ of whole body after being absorbed in the body, and most antibiotics is excreted by ight soil, the part antibiotic residue is also arranged in vivo.After being excreted in the environment, the stable antibiotic of properties still can have a very long time, thereby cause the medicament residue in the environment, these remaining medicines are slowly accumulated in human body and other plant body by livestock products and environment, finally come together in human body with various approach, cause human body to produce a large amount of antibody-resistant bacterium, lose the resistance to some disease, or because accumulating in a large number body is produced toxic action.
Summary of the invention
The purpose of this invention is to provide a kind of sheep tailored version animal nutrient and preparation method thereof, overcome effectively that the resistance to the action of a drug that a large amount of unreasonable use antibiotic of prior art cause is serious, immunity degradation, autogenous infection and suprainfection, the technological deficiency such as human health that jeopardizes.
For achieving the above object, the invention provides a kind of sheep tailored version animal nutrient, comprise the constituent of following percentage by weight: fulvic acid 40%-60%, Total Flavonoids from Taraxacum mongolicum 0.1%-0.3% and bitter bean pulp fermentation culture 39.7%-59.7%.
On the basis of technique scheme, described bitter bean pulp fermentation culture comprises: bitter dregs of beans substrate, S. cervisiae, bacillus subtilis and bacillus licheniformis.
Further, described bitter dregs of beans substrate comprises the constituent of following percentage by weight: bitter dregs of beans 90%-97%, corn flour 2%-8% and beet molasses 0.5%-2%.
Preferably, the number of viable of S. cervisiae is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu, the number of viable summation of bacillus subtilis and described bacillus licheniformis is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu.
The present invention also provides a kind of preparation method of sheep tailored version animal nutrient, comprising:
Extract fulvic acid;
Prepare bitter bean pulp fermentation culture;
Be that the described bitter bean pulp fermentation culture that the described fulvic acid of 40%-60%, Total Flavonoids from Taraxacum mongolicum that percentage by weight is 0.1%-0.3% and percentage by weight are 39.7%-59.7% mixes with percentage by weight.
On the basis of technique scheme, described extraction fulvic acid specifically comprises:
Fulvic acid is extracted raw meal be broken to the 60-80 order, the fulvic acid after pulverizing is extracted raw material by 1: it is in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, in temperature 60 C-80 ℃ lower the extraction 2-3 hour;
The slagging-off of said extracted liquid is rear by 100: the part by weight adding mass concentration of 1-2 is 30% hydrogen peroxide, in temperature 70 C-80 ℃ lower activation 1.5-2 hour, obtains activating solution;
With molar concentration be the hydrochloric acid solution of 1mol/L and mass concentration be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants, with above-mentioned activating solution and described compound precipitants by 1: the volume ratio of 50-80 mixes, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid.
It is brown coal, weathered coal or peat that described fulvic acid extracts raw material.
Further, the bitter bean pulp fermentation culture of described preparation is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled, mix afterwards and descended aerobic fermentation 16-36 hour more first anaerobic fermentation 38-60 hour in 20 ℃-40 ℃ of temperature.
Preferably, described bitter dregs of beans substrate comprises the constituent of following percentage by weight: bitter dregs of beans 90-97%, corn flour 2-8%, beet molasses 0.5-2%.Described composite microbial inoculant liquid comprises: S. cervisiae, bacillus subtilis and bacillus licheniformis, the number of viable of S. cervisiae is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu, the number of viable summation of bacillus subtilis and described bacillus licheniformis is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu.
The invention provides a kind of sheep tailored version animal nutrient and preparation method thereof, have the following advantages:
1, improves functions of intestines and stomach, enhancing immunity of organisms.After fulvic acid enters alimentary canal in the sheep tailored version animal nutrient of the present invention, can stimulate the coat of the stomach mucous membrane to increase gastric secretion, increase appetite; Because fulvic acid has surface-active, can promote nutrients to enter sooner body again, improve absorptivity and conversion ratio; Can also stimulate beneficial microorganism growth in the sheep stomach and intestine, suppress the breeding of spoilage organisms, prevent the poisoning of sheep hydrochloric acid in gastric juice; Fulvic acid has stronger absorption and complexing power, can effectively adsorb poisonous and harmful substance and bacteriotoxin in the stomach, has detoxifcation, alleviates excreta stink and significantly astringing to arrest diarrhea effect, is conducive to the improvement of stable breeding drylot feeding environment.Simultaneously, fulvic acid and Total Flavonoids from Taraxacum mongolicum can promote the activity of multiple probiotic (S. cervisiae, bacillus subtilis, bacillus licheniformis etc.) propagation and enhancing plurality of enzymes (polyphenol oxidase, peroxidase, ascorbic acid oxidase etc.) in the sheep tailored version animal nutrient of the present invention, strengthen body immunocompetent cell and body immune system rejection ability, body immunity is improved.
2, reduce the gastrointestinal disease incidence of disease, put on weight, improve quality of mutton.Fulvic acid and Total Flavonoids from Taraxacum mongolicum can promote multiple probiotic (S. cervisiae, bacillus subtilis, bacillus licheniformis etc.) propagation in the sheep tailored version animal nutrient of the present invention, satisfy the activity of microorganism in the stomach, promote stomach physiogeny, strengthen the to external world adaptive capacity of environment of sheep, the gastroenteritis of sheep, lamb dysentery etc. had good prevention effect, simultaneously can also increase the body weight of sheep and significantly improve quality of mutton, so that fine and tender taste, meat flavour are delicious.
3, safe ready.Sheep tailored version animal nutrient of the present invention only need be admixed in proportion to feed in the feed and get final product, and is simple and convenient; Simultaneously, sheep tailored version animal nutrient of the present invention does not contain antibiotic and/or antibiotics material, and the raiser can relievedly use sheep, can slowly not accumulate in human body by livestock products and environment, and the mankind are caused healthy hidden danger.
4, stable performance, easily preservation.The shortcoming of probiotic (S. cervisiae, bacillus subtilis, bacillus licheniformis etc.) is poor heat stability in the sheep tailored version animal nutrient of the present invention, is in modified, granulation, the heat treatment such as expanded or long-term storage all causes inactivation easily under normal temperature condition; And the active ingredients such as fulvic acid, Total Flavonoids from Taraxacum mongolicum have also been added simultaneously in the sheep tailored version animal nutrient of the present invention, become the composite parts product, this compound product can remedy each composition deficiency to each other in the process of processing, preservation, use, make the effect maximization of sheep tailored version animal nutrient of the present invention.
Below by embodiment, technical scheme of the present invention is described in further detail.
Description of drawings
Fig. 1 is preparation method's flow chart of sheep tailored version animal nutrient of the present invention;
Fig. 2 is the flow chart that the present invention extracts fulvic acid.
The specific embodiment
Further specify the technical scheme of sheep tailored version animal nutrient of the present invention below by specific embodiment.
Sheep tailored version animal nutrient the first embodiment of the present invention
Sheep tailored version animal nutrient comprises the constituent of following percentage by weight: fulvic acid 40%, Total Flavonoids from Taraxacum mongolicum 0.3% and bitter bean pulp fermentation culture 59.7%.Described bitter bean pulp fermentation culture comprises: percentage by weight is that 90% bitter dregs of beans, percentage by weight are that 8% corn flour, percentage by weight are 2% beet molasses, and the number of viable of S. cervisiae is 1 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu, the number of viable summation of bacillus subtilis and bacillus licheniformis is 10 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu.
Sheep tailored version animal nutrient the second embodiment of the present invention
Sheep tailored version animal nutrient comprises the constituent of following percentage by weight: fulvic acid 50%, Total Flavonoids from Taraxacum mongolicum 0.15% and bitter bean pulp fermentation culture 49.85%.Described bitter bean pulp fermentation culture comprises: percentage by weight is that 95% bitter dregs of beans, percentage by weight are that 4% corn flour, percentage by weight are 1% beet molasses, and the number of viable of S. cervisiae is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu, the number of viable summation of bacillus subtilis and bacillus licheniformis is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu.
Sheep tailored version animal nutrient the 3rd embodiment of the present invention
Sheep tailored version animal nutrient comprises the constituent of following percentage by weight: fulvic acid 60%, Total Flavonoids from Taraxacum mongolicum 0.1% and bitter bean pulp fermentation culture 39.9%.Described bitter bean pulp fermentation culture comprises: percentage by weight is that 97% bitter dregs of beans, percentage by weight are that 2% corn flour, percentage by weight are 1% beet molasses, and the number of viable of S. cervisiae is 10 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu, the number of viable summation of bacillus subtilis and bacillus licheniformis is 1 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu.
Sheep tailored version animal nutrient the 4th embodiment of the present invention
Sheep tailored version animal nutrient comprises the constituent of following percentage by weight: fulvic acid 60%, Total Flavonoids from Taraxacum mongolicum 0.3% and bitter bean pulp fermentation culture 39.7%.Described bitter bean pulp fermentation culture comprises: percentage by weight is that 95% bitter dregs of beans, percentage by weight are that 4.5% corn flour, percentage by weight are 0.5% beet molasses, and the number of viable of S. cervisiae is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu, the number of viable summation of bacillus subtilis and bacillus licheniformis is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8Cfu.
In the technical scheme of the invention described above sheep tailored version animal nutrient the first embodiment to the four embodiment, described bitter dregs of beans is the residue of Sophora alopecuroide after extracting matrine, available from Inner Mongol Huimin biotech firm.
Sheep tailored version animal nutrient of the present invention can be admixed the sheep of feeding in the feed according to the ratio of 3-5 kg/ton and get final product.
Technical scheme by the invention described above sheep tailored version animal nutrient the first embodiment to the four embodiment can be found out, compared with prior art, sheep tailored version animal nutrient of the present invention have improve functions of intestines and stomach, strengthen immunity of organisms, reduce the gastrointestinal disease incidence of disease, put on weight, improve quality of mutton, safe ready, stable performance, the remarkable result easily preserved.
Fig. 1 is preparation method's flow chart of sheep tailored version animal nutrient of the present invention, is specially:
Fig. 2 is the flow chart that the present invention extracts fulvic acid, and in technical scheme shown in Figure 1, step 1 is specially:
Step 11, fulvic acid is extracted raw meal be broken to the 60-80 order, the fulvic acid after pulverizing is extracted raw material by 1: it was in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, in temperature 60 C-80 ℃ lower extraction 2-3 hour;
The part by weight adding mass concentration of step 12, the slagging-off of said extracted liquid is rear by 100: 1-2 is 30% hydrogen peroxide, in temperature 70 C-80 ℃ lower activation 1.5-2 hour, obtains activating solution;
Step 13, with molar concentration be the hydrochloric acid solution of 1mol/L and mass concentration be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants, with above-mentioned activating solution and described compound precipitants by 1: the volume ratio of 50-80 mixes, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid.
On the basis of technique scheme, it is brown coal, weathered coal or peat that described fulvic acid extracts raw material.Further, the bitter bean pulp fermentation culture of described preparation is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled, mix afterwards and descended aerobic fermentation 16-36 hour more first anaerobic fermentation 38-60 hour in 20 ℃-40 ℃ of temperature.Preferably, described bitter dregs of beans substrate comprises the constituent of following percentage by weight: bitter dregs of beans 90-97%, corn flour 2-8%, beet molasses 0.5-2%.Described composite microbial inoculant liquid comprises: S. cervisiae, bacillus subtilis and bacillus licheniformis, the number of viable of S. cervisiae is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu, the number of viable summation of bacillus subtilis and described bacillus licheniformis is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu.
Preparation method first embodiment of sheep tailored version animal nutrient of the present invention
Step 101, brown coal powder is broken to 70 orders, it is that extraction is 2.5 hours under temperature 70 C, obtains extract in 4% the sodium hydroxide solution that the brown coal after pulverizing are added mass concentration by 1: 8 part by weight;
Step 102, be 30% hydrogen peroxide with said extracted liquid through adding mass concentration by 100: 1.5 part by weight after the centrifugation slagging-off, activate 2 hours in 75 ℃ of temperature are lower, obtain activating solution;
Step 103, be that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 1 volume ratio for the hydrochloric acid solution of 1mol/L and mass concentration with molar concentration, above-mentioned activating solution is mixed with the volume ratio of described compound precipitants by 1: 50, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid of PH=6.5;
Step 104, bitter dregs of beans substrate and water are become reconciled by 2: 1 weight ratio, composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 1: 5 ml/kg ratio, mix afterwards and descended first anaerobic fermentations 60 hours in 20 ℃ of temperature, aerobic fermentation is 16 hours again, obtain bitter bean pulp fermentation culture, number of viable is 1 * 10 in the bitter bean pulp fermentation culture of every gram
8The number of viable summation is 10 * 10 in the S. cervisiae of cfu, the bitter bean pulp fermentation culture of every gram
8The bacillus subtilis of cfu and bacillus licheniformis, wherein, described bitter dregs of beans substrate comprises: percentage by weight is that 90% bitter dregs of beans, percentage by weight are that 8% corn flour, percentage by weight are 2% beet molasses, and described composite microbial inoculant liquid comprises: number of viable is 1 * 10 in every milliliter of composite microbial inoculant liquid
10The number of viable summation is 10 * 10 in the S. cervisiae of cfu, the every milliliter of composite microbial inoculant liquid
10The bacillus subtilis of cfu and bacillus licheniformis;
Step 105, be that 40% described feed grade fulvic acid, percentage by weight are that 0.3% Total Flavonoids from Taraxacum mongolicum and percentage by weight are that 59.7% described bitter bean pulp fermentation culture mixes with percentage by weight.
Preparation method second embodiment of sheep tailored version animal nutrient of the present invention
Step 201, weathered coal is crushed to 60 orders, it is that extraction is 2 hours under temperature 60 C, obtains extract in 3% the sodium hydroxide solution that the weathered coal after pulverizing is added mass concentration by 1: 10 part by weight;
Step 202, be 30% hydrogen peroxide with said extracted liquid through adding mass concentration by 100: 1 part by weight after the centrifugation slagging-off, under temperature 70 C, activate 1.5 hours, obtain activating solution;
Step 203, be that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 2 volume ratio for the hydrochloric acid solution of 1mol/L and mass concentration with molar concentration, above-mentioned activating solution is mixed with the volume ratio of described compound precipitants by 1: 70, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid of PH=6.8;
Step 204, bitter dregs of beans substrate and water are become reconciled by 2: 1 weight ratio, composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 1: 8 ml/kg ratio, mix afterwards and descended first anaerobic fermentations 48 hours in 25 ℃ of temperature, aerobic fermentation is 24 hours again, obtain bitter bean pulp fermentation culture, number of viable is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8The number of viable summation is 5 * 10 in the S. cervisiae of cfu, the bitter bean pulp fermentation culture of every gram
8The bacillus subtilis of cfu and bacillus licheniformis, wherein, described bitter dregs of beans substrate comprises: percentage by weight is that 95% bitter dregs of beans, percentage by weight are that 4% corn flour, percentage by weight are 1% beet molasses, and described composite microbial inoculant liquid comprises: number of viable is 5 * 10 in every milliliter of composite microbial inoculant liquid
10The number of viable summation is 5 * 10 in the S. cervisiae of cfu, the every milliliter of composite microbial inoculant liquid
10The bacillus subtilis of cfu and bacillus licheniformis;
Step 205, be that 50% described feed grade fulvic acid, percentage by weight are that 0.15% Total Flavonoids from Taraxacum mongolicum and percentage by weight are that 49.85% described bitter bean pulp fermentation culture mixes with percentage by weight.
Preparation method the 3rd embodiment of sheep tailored version animal nutrient of the present invention
Step 301, peat dust is broken to 80 orders, it is in 2% the sodium hydroxide solution that the peat after pulverizing is added mass concentration by 1: 9 part by weight, in 80 ℃ of lower extractions of temperature 3 hours, obtains extract;
Step 302, be 30% hydrogen peroxide with said extracted liquid through adding mass concentration by 100: 2 part by weight after the centrifugation slagging-off, activate 1.8 hours in 80 ℃ of temperature are lower, obtain activating solution;
Step 303, be that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 3 volume ratio for the hydrochloric acid solution of 1mol/L and mass concentration with molar concentration, above-mentioned activating solution is mixed with the volume ratio of described compound precipitants by 1: 80, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid of PH=7;
Step 304, bitter dregs of beans substrate and water are become reconciled by 2: 1 weight ratio, composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 1: 10 ml/kg ratio, mix afterwards and descended first anaerobic fermentations 38 hours in 40 ℃ of temperature, aerobic fermentation is 36 hours again, obtain bitter bean pulp fermentation culture, number of viable is 10 * 10 in the bitter bean pulp fermentation culture of every gram
8The number of viable summation is 1 * 10 in the S. cervisiae of cfu, the bitter bean pulp fermentation culture of every gram
8The bacillus subtilis of cfu and bacillus licheniformis, wherein, described bitter bean pulp fermentation culture comprises: percentage by weight is that 97% bitter dregs of beans, percentage by weight are that 2% corn flour, percentage by weight are 1% beet molasses, and described composite microbial inoculant liquid comprises: number of viable is 10 * 10 in every milliliter of composite microbial inoculant liquid
10The number of viable summation is 1 * 10 in the S. cervisiae of cfu, the every milliliter of composite microbial inoculant liquid
10The bacillus subtilis of cfu and bacillus licheniformis;
Step 305, be that 60% described feed grade fulvic acid, percentage by weight are that 0.1% Total Flavonoids from Taraxacum mongolicum and percentage by weight are that 39.9% described bitter bean pulp fermentation culture mixes with percentage by weight.
Preparation method the 4th embodiment of sheep tailored version animal nutrient of the present invention
Step 401, weathered coal is crushed to 60 orders, it is that extraction is 2 hours under temperature 60 C, obtains extract in 3% the sodium hydroxide solution that the weathered coal after pulverizing is added mass concentration by 1: 10 part by weight;
Step 402, be 30% hydrogen peroxide with said extracted liquid through adding mass concentration by 100: 1 part by weight after the centrifugation slagging-off, under temperature 70 C, activate 1.5 hours, obtain activating solution;
Step 403, be that to be 0.2 ‰ polyacrylamide solution be mixed and made into compound precipitants by 10: 2 volume ratio for the hydrochloric acid solution of 1mol/L and mass concentration with molar concentration, above-mentioned activating solution is mixed with the volume ratio of described compound precipitants by 1: 70, precipitation is also separated and is removed palm fibre, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid of PH=6.8;
Step 404, bitter dregs of beans substrate and water are become reconciled by 2: 1 weight ratio, composite microbial inoculant liquid is inoculated in the bitter dregs of beans substrate that water becomes reconciled in 1: 8 ml/kg ratio, mix afterwards and descended first anaerobic fermentations 48 hours in 25 ℃ of temperature, aerobic fermentation is 24 hours again, obtain bitter bean pulp fermentation culture, number of viable is 5 * 10 in the bitter bean pulp fermentation culture of every gram
8The number of viable summation is 5 * 10 in the S. cervisiae of cfu, the bitter bean pulp fermentation culture of every gram
8The bacillus subtilis of cfu and bacillus licheniformis, wherein, described bitter dregs of beans substrate comprises: percentage by weight is that 95% bitter dregs of beans, percentage by weight are that 4.5% corn flour, percentage by weight are 0.5% beet molasses, and described composite microbial inoculant liquid comprises: number of viable is 5 * 10 in every milliliter of composite microbial inoculant liquid
10The number of viable summation is 5 * 10 in the S. cervisiae of cfu, the every milliliter of composite microbial inoculant liquid
10The bacillus subtilis of cfu and bacillus licheniformis;
Step 405, be that 60% described feed grade fulvic acid, percentage by weight are that 0.3% Total Flavonoids from Taraxacum mongolicum and percentage by weight are that 39.7% described bitter bean pulp fermentation culture mixes with percentage by weight.
In the technical scheme of preparation method first embodiment to the of the invention described above sheep tailored version animal nutrient four embodiment, described bitter dregs of beans is the residue of Sophora alopecuroide after extracting matrine, available from Inner Mongol Huimin biotech firm.
Can admix the sheep of feeding in the feed according to the ratio of 3-5 kg/ton by the sheep tailored version animal nutrient of the preparation method of the invention described above sheep tailored version animal nutrient preparation gets final product.
Technical scheme by preparation method the first embodiment to the four embodiment of the invention described above sheep tailored version animal nutrient can be found out, compared with prior art, by the sheep tailored version animal nutrient of the preparation method of sheep tailored version animal nutrient of the present invention preparation have improve functions of intestines and stomach, strengthen immunity of organisms, reduce the gastrointestinal disease incidence of disease, put on weight, improve quality of mutton, safe ready, stable performance, the remarkable result easily preserved.
The remarkable efficacy of sheep tailored version animal nutrient of the present invention is described below by concrete test.
12 of the first-filial generation mutton sheeps of northeast fine-wool sheep * German Mutton merino are selected in test, be divided into control group and test group, 6 of every processed group, with reference to mutton sheep feeding standard (China, 2004) preparation basal diet, take the sheep of the basal diet of feeding as control group, sheep tailored version animal nutrient of the present invention is added into according to the ratio of 5 kg/ton consists of the test daily ration in the basal diet, take the sheep of feeding experiment daily ration as test group.Experimental period is 60 days.
Result of the test shows:
1, the material anharmonic ratio significantly reduces.Within whole experimental period, the material anharmonic ratio of test group contrast group has reduced by 11%.
2, Daily gain significantly improves.Within whole experimental period, the average daily gain rate of test group contrast group has improved 14.6%.
In addition, lot of experimental data proves, behind the edible feed that adds sheep tailored version animal nutrient of the present invention, the incidence of disease of gastrointestinal disease (such as gastroenteritis, lamb dysentery etc.) significantly reduces sheep for a long time, and the mutton fine and tender taste after butchering, meat flavour are delicious.
Sheep tailored version animal nutrient of the present invention have improve functions of intestines and stomach, strengthen immunity of organisms, reduce the gastrointestinal disease incidence of disease, put on weight, improve quality of mutton, safe ready, stable performance, the remarkable result easily preserved.
It should be noted last that, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although with reference to preferred embodiment the present invention is had been described in detail, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not break away from the spirit and scope of technical solution of the present invention.
Claims (3)
1. a sheep tailored version animal nutrient is characterized in that, comprises the constituent of following percentage by weight: fulvic acid 40%-60%, Total Flavonoids from Taraxacum mongolicum 0.1%-0.3% and bitter bean pulp fermentation culture 39.7%-59.7%; Described bitter bean pulp fermentation culture comprises: bitter dregs of beans substrate, S. cervisiae, bacillus subtilis and bacillus licheniformis; Preparing described bitter bean pulp fermentation culture is specially: with composite microbial inoculant liquid in 1-5: 10 ml/kg ratio is inoculated in the bitter dregs of beans substrate that water becomes reconciled, mix afterwards and descended aerobic fermentation 16-36 hour more first anaerobic fermentation 38-60 hour in 20 ℃-40 ℃ of temperature; Described bitter dregs of beans substrate comprises the constituent of following percentage by weight: bitter dregs of beans 90%-97%, corn flour 2%-8% and beet molasses 0.5%-2%; Described composite microbial inoculant liquid comprises: S. cervisiae, bacillus subtilis and bacillus licheniformis, the number of viable of S. cervisiae is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu, the number of viable summation of bacillus subtilis and described bacillus licheniformis is 1 * 10 described in the described bitter bean pulp fermentation culture of every gram
8-10 * 10
8Cfu.
2. sheep tailored version animal nutrient according to claim 1 is characterized in that,
It is brown coal, weathered coal or peat that fulvic acid extracts raw material;
Extracting fulvic acid specifically comprises:
Fulvic acid is extracted raw meal be broken to the 60-80 order, the fulvic acid after pulverizing is extracted raw material by 1: it is in the sodium hydroxide solution of 2%-4% that the part by weight of 8-10 adds mass concentration, in temperature 60 C-80 ℃ lower the extraction 2-3 hour;
The slagging-off of said extracted liquid is rear by 100: the part by weight adding mass concentration of 1-2 is 30% hydrogen peroxide, in temperature 70 C-80 ℃ lower activation 1.5-2 hour, obtains activating solution;
With molar concentration be the hydrochloric acid solution of 1mol/L and mass concentration be 0.2 ‰ polyacrylamide solution by 10: the volume ratio of 1-3 is mixed and made into compound precipitants, with activating solution and described compound precipitants by 1: the volume ratio of 50-80 mixes, precipitation is also separated and is removed hymatomalenic acid, pyrotomalenic acid, with the clarified solution concentrate drying, obtain the feed grade fulvic acid.
3. a method for preparing claim 1 or 2 described sheep tailored version animal nutrients is characterized in that, comprising:
Extract fulvic acid;
Prepare bitter bean pulp fermentation culture;
Be that the described bitter bean pulp fermentation culture that the described fulvic acid of 40%-60%, Total Flavonoids from Taraxacum mongolicum that percentage by weight is 0.1%-0.3% and percentage by weight are 39.7%-59.7% mixes with percentage by weight.
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| CN102669485B (en) * | 2012-06-11 | 2013-11-06 | 内蒙古永业农丰生物科技有限责任公司 | Animal biotin special for broilers and preparing method thereof |
| CN102669486B (en) * | 2012-06-11 | 2014-02-26 | 内蒙古永业农丰生物科技有限责任公司 | Special animal biogen for shrimps and preparation method thereof |
| CN102669484B (en) * | 2012-06-11 | 2013-11-06 | 内蒙古永业农丰生物科技有限责任公司 | Special animal biogen for laying hens and preparation method of special animal biogen |
| CN102669487B (en) * | 2012-06-11 | 2013-11-06 | 内蒙古永业农丰生物科技有限责任公司 | Animal biogen special for pigs and preparation method thereof |
| CN102669436B (en) * | 2012-06-11 | 2013-12-11 | 内蒙古永业农丰生物科技有限责任公司 | Special animal life element for fish and preparation method for same |
| CN108391748A (en) * | 2018-02-07 | 2018-08-14 | 于长青 | A kind of liquid feed additive and preparation method thereof improving poultry intestinal health |
| CN109329602A (en) * | 2018-12-04 | 2019-02-15 | 李仁帅 | A kind of weaned lamb cultivation full-valence pellet feed and preparation method thereof |
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