CN104431621A - Compound premix for aquatic products and preparation method of compound premix - Google Patents
Compound premix for aquatic products and preparation method of compound premix Download PDFInfo
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Abstract
The invention relates to a compound premix for aquatic products. The compound premix is prepared from the following raw materials based on the percentage by mass of air dried substances: 10%-15% of a seaweed organism active extract, 5%-10% of a composite acidifier, 5%-10% of compound probiotics powder and the balance of a carrier. The invention also provides a preparation method. The method comprises the following steps: (1) carrying out superfine grinding on raw materials of the seaweed organism active extract, carrying out ultrasonic-microwave synergistic extraction, filtering, wherein filtrate is taken as an extract liquid, carrying out enzymolysis and fermentation on filter residues, so as to obtain a fermentation liquid, respectively carrying out freeze drying on the extract liquid and the fermentation liquid, and then mixing at the ratio; and (2) carrying out superfine grinding on the carrier, and then evenly mixing the ground carrier with the composite acidifier, the compound probiotics powder and the seaweed organism active extract. According to the premix disclosed by the invention, marine algae resources can be effectively utilized; protease can be secreted; digestive absorption of protein is facilitated; the anti-stress ability and the disease resistance of organisms are enhanced; and the growth speed of aquatic livestock is improved, so that the culture cost is reduced and the culture benefits are significantly improved.
Description
Technical field
The present invention relates to feeding additive aquatic animal, particularly relate to a kind of aquatic products compound premix and preparation method thereof.
Background technology
Aquatic products are one of food be loved by the people, and aquaculture brings good economic benefit to fishmen.But along with the deterioration of breeding environment, the development of the diseases such as bacterium, aquaculture aquaculture in recent years receives serious blow.The problems such as particularly fishes and shrimps immunity of organisms is low, growth is slow, morbidity is severe, survival rate is low, have had influence on the sound development of culture fishery.Aquatic feeds is as one of means of production most important in aquaculture (having accounted for the 50-70% of totle drilling cost), and the immune disease-resistance power, the speed of growth, feed coefficient, cultivation survival rate, culture benefit etc. of its quality parameter on fishes and shrimps body all have important impact.
In recent years, along with population sharply increases, plough and reduce in a large number, feedstuff is in short supply has become the serious problem affecting aquaculture fast development, contradiction also more serious that man and animal strives grain.Researcher is being devoted to find the low feedstuff of a kind of cost always.Marine alga is a kind of feed resource of low cost, and marine alga is as feedstuff and feed addictive, and its potentiality to be exploited is very large.In addition, marine alga contains abundant algal polysaccharides, protein, fat, vitamin, mineral matter and has the physiological activator of special efficacy, it is the raw material storage of food, medicine, feed, sea-plant is nutritious, containing various bioactivators, there is biologically actives such as strengthening immunity of organisms, disease-resistant, antiviral, growth promoting effects.
Although marine alga has many good qualities as raw material of aquatic product feed stuff or feed addictive, come with some shortcomings in the aquatic feeds sector application of China, main manifestations is: (1) lacks system to tangleweed at present and in depth studies; (2) digestion power of cultivated animals to algal polysaccharides is poor, easily causes the waste of resource, and production technology is a great problem that seaweed fodder commercial development faces; (3) in tangleweed, the composition of the nutriment of various algae and content difference are very large, how scientific compatibility reasonable combination, and play its special efficacy, reach safe and efficient use, the object improving breeding performonce fo animals is also a difficult problem.
In sum, in aquatic feeds and additive agent field, both cost is low to find one, the raw material that can improve again aquatic livestock immunity in the urgent need to.Although it is low that marine alga has cost, nutritious feature, but, also fail at present to be used in efficiently and safely in aquatic livestock additive.
Summary of the invention
For above deficiency, the invention provides one and effectively can utilize marine algae resource, make feed cost reduce and can improve a kind of aquatic products compound premix of animal survival rate and cultivation efficiency and preparation method thereof.
The present invention utilizes specific marine alga composition, specifically process, obtain easy to digest, good palatability and there is the seaweed bio activity extract of the function such as antibacterial, and coordinating and probio compound, acidulant compound, obtaining good stability, the obvious compound premix of economic worth.
A kind of aquatic products compound premix, with air dry matter amount percentages, comprise 10-15% seaweed bio activity extract, the compound acidulant of 5-10%, 5-10% compound probiotic powder, all the other are carrier.
According to dry measure weight parts, above-mentioned seaweed bio activity extract is preferably obtained by following raw material: split 5 ~ 10 parts, kettle algae, 10 ~ 20 parts, sea-tangle, undaria pinnitafida 5 ~ 15 parts, asparagus 35 ~ 50 parts, Enteromorpha 15 ~ 25 parts, laver 5 ~ 15 parts.
According to dry measure weight parts, above-mentioned compound acidulant preferably comprises sodium butyrate 50 ~ 70 parts, sodium Diacetate 10 ~ 20 parts, potassium diformate 20 ~ 30 parts.
According to dry measure weight parts, above-mentioned compound probiotic powder preferably includes bacillus subtilis 30 ~ 50 parts, lactobacillus acidophilus 30 ~ 50 parts, clostridium butyricum 20 ~ 30 parts, streptococcus fecalis 5-10 part.
According to dry measure weight parts, above-mentioned carrier comprises silica 10 ~ 20 part, corncob 30 ~ 50 parts, starch 40 ~ 50 parts.
The physical and chemical index of above-mentioned aquatic products compound premix is: proterties is light yellow or yellowish-brown powder; Granularity is that 95-98% is by 200 mesh standard sieves; The uniformity is the 9.0-10.0% coefficient of variation; Moisture 7.0-8.0%.
The operating weight of above-mentioned compound premix in aquatic feeds is than being 1-2% (mass percent).
The present invention also provides a kind of method preparing above-mentioned aquatic products compound premix, comprising:
(1) preparation of seaweed bio activity extract; By the raw material ultramicro grinding of seaweed bio activity extract, ultrasonic-microwave is collaborative to be extracted and after filtering, filtrate is as extract, enzymolysis is carried out to filter residue, fermentation obtains zymotic fluid, proportionally be mixed to get extract of active substances after extract and zymotic fluid are carried out freeze drying respectively, it is extract after freeze drying that the ratio of mixing is preferably by dry measure weight ratio: the zymotic fluid=1:100 after freeze drying;
(2) by carrier ultramicro grinding, then mix with compound acidulant, compound probiotic powder and seaweed bio activity extract, obtain compound premix.
Preferably, the enzymolysis of the step (1) of above-mentioned preparation method carries out enzymolysis for adding complex enzyme, and complex enzyme is the complex enzyme be made up of cellulase, pectase, papain, zytase.
Preferred further, the mass ratio of above-mentioned complex enzyme cellulase, pectase, papain, zytase is 3:2:3:2, and the mass ratio of described complex enzyme and reaction substrate is 3 ~ 4:100.
Preferred further, the reaction condition of above-mentioned complex enzyme zymohydrolysis is preferably complex enzyme: reaction substrate (raw material of seaweed bio activity extract) mass ratio 3 ~ 4:100, and reaction temperature is 45 ~ 55 DEG C, and enzymolysis time is 100 ~ 150 minutes.
Preferred further, the reaction condition more preferably complex enzyme of above-mentioned complex enzyme zymohydrolysis: reaction substrate (raw material of seaweed bio activity extract) mass ratio 4:100, reaction temperature is 50 DEG C, and enzymolysis time is 120 minutes.
Preferably, the ultrasonic-microwave of the step (1) of above-mentioned preparation method is worked in coordination with to be extracted as in the seaweed bio activity extract raw material after ultramicro grinding and is added ethanol-hydrogen chloride, carry out the collaborative extraction of ultrasonic-microwave, the collaborative optimum condition extracted is: hyperacoustic power is 100W, extraction time 40min, Extracting temperature 45 DEG C, solid-liquid ratio 1g: 3mL, microwave power 250 ~ 400W.
Preferably, the fermentation of the step (1) of above-mentioned preparation method after enzymolysis, adds lactic acid bacteria and yeast ferments, ferment and obtain zymotic fluid in 10-12 hour, the addition of lactic acid bacteria and yeast is more preferably: lactic acid bacteria is 100,000,000 every milliliter, dusty yeast: total liquid is 1:100 (mass volume ratio).
Preferably, above-mentioned compound probiotic powder can the preparation of commercially available purchase raw material, but preferably prepares voluntarily, and preparation method is preferably: through liquid fermentation and culture, centrifugal, concentrate after add freeze drying protectant, vacuum freeze drying obtains compound probiotic powder.
Wherein, above-mentioned compound probiotic powder measures the viable count in every gram of bacterium powder according to international GB/T4789.35--2003 method, and mensuration obtains viable count in bacterium powder and is greater than 10
8cFU/g.
Wherein, the freeze drying protectant composition in above-mentioned compound probiotic powder preparation is preferably: sucrose 16g, sorbierite 16g, skimmed milk power 19g, and distilled water adds to 250mL; Through liquid fermentation and culture, centrifugal, concentrated after, the adding proportion of the freeze drying protectant added be concentrated after thalline: freeze drying protectant is 1g:100mL, and frozen-dried protective rate reaches more than 75%.
The invention provides the compound premix of the composite probio of seaweed bio activity extract used in a kind of aquatic feeds, digestive utilization ratio can be improved, improve palatability after adding in aquatic feeds, aquatic animal to grow rapidly, feed water stability is good, reduce feed coefficient, improve the high and culture benefit of survival rate.
Beneficial effect of the present invention is:
(1) Ultrasonic-microwave synergistic extraction technology is adopted to carry out the extraction of seaweed bio active material, ultrasonic wave and microwave two kinds of electromagnetic wave phases are combined, make full use of the invigoration effect of ultrasonic wave vibration and the high energy effect of microwave, overcome the deficiency utilizing merely ultrasonic wave or microwave abstracting, achieve quick, high efficiency extraction to marine alga active ingredient under low-temperature atmosphere-pressure condition, and do not destroy the wherein active ingredient such as seeweed polyphenol;
(2) lactic acid bacteria adopted in the zymotechnique of seaweed bio activity extract and saccharomycete, can secrete antibiotin class material, produces antagonism to pathogenic entero becteria; Acid producing ability is strong, can produce lactic acid and acetic acid, in premix, also enhance the interpolation of the acidulants such as SCFA simultaneously, be both conducive to effectively suppressing fishes and shrimps enteron aisle common pathogen, promoted again the reparation of intestinal tract injury in enteron aisle; The utilization rate of calcium, phosphorus, iron can be improved, promote again the absorption of iron and vitamin D; Can produce vitamin K and Cobastab, be fishes and shrimps body replenishing vitamins; Can also extracellular proteinase, promote digesting and assimilating of protein, enhance body resisting stress and anti-disease ability, improve the speed of growth, thus reduce aquaculture cost;
(3) the compound probiotic powder used in premix of the present invention, acid resistance is strong, grows in the environment that can not be able to grow other lactic acid bacterias, and colonization ability is strong, and namely a large amount of viable bacteria can enter enteron aisle smoothly and play prebiotic function; Use the medicated premixs such as the antibiotics growth accelerator in the alternative pre existing mix product such as probio and acidulant, noresidue, pollution-free, do not produce drug resistance, the pathogenic bacteria resistance to drugs that solution causes because of additives such as Long-Time Service antibiotic is strong, the problem that animal immunizing power declines;
(4) material combination of the seaweed bio activity extract of premix of the present invention have passed through the compatibility checking of science, and have passed through ultramicro grinding, enzymatic hydrolysis and fermentation PROCESS FOR TREATMENT, degrade part SNSP, containing various abundant amino acid, ALANINE wherein, L-Methionine, histidine, arginine, ornithine etc. have strong food calling effect to prawn, good palatability, digestibility is high; Our experiments show that, fishes and shrimps feeding is added to the feed of compound premix of the present invention, good palatability, Feeding Velocity is fast, digests, decreases the molten mistake of feed in water, fast growth, and it is short to go out the pool time;
(5) the seaweed bio activity extract owing to obtaining in the present invention, algal polysaccharides wherein has the effect improving animal immunizing power, the acrylic acid contained, terpenes, bromination phenols and some sulfur-containing compound have antibacterial action, and sulfated polysaccharide has antivirus action; Wherein split kettle algae and enhance the high unsaturated fatty acids such as DHA, promote that cranial nerve is grown and anti-oxidant scavenging activated oxygen ability; Feeding adds the fishes and shrimps of this compound premix feed, and anti-stress ability is strong, and immunity of organism premunition improves, and survival rate is high, and culture benefit is remarkable;
(6) because the seaweed bio activity extract obtained in the present invention is all containing a certain amount of biological viscosity jelly, after processing, the natural high-quality binder of feed can be become, the stability of fish and shrimp feed can be strengthened, prevent water pollution.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described.
Embodiment 1
A kind of aquatic products compound premix, with air-dry thing parts by weight, each component is: 15 parts of seaweed bio activity extracts, 8 parts of compound acidulants, 10 parts of compound probiotic powder, 66 parts of carriers.
Wherein, seaweed bio activity extract is prepared by marine alga composition and obtains, and marine alga composition is: split kettle algae 10 weight portion, sea-tangle 10 weight portion, undaria pinnitafida 10 weight portion, asparagus 40 weight portion, Enteromorpha 20 weight portion, laver 10 weight portion.
Wherein, compound probiotic powder calculates according to dry measure parts by weight, is made up of bacillus subtilis 40 parts, lactobacillus acidophilus 30 parts, clostridium butyricum 20 parts, streptococcus fecalis 10 parts.
Wherein, compound acidulant calculates according to dry measure parts by weight, is made up of sodium butyrate 50 parts, sodium Diacetate 20 parts, potassium diformate 30 parts.
Wherein, carrier calculates according to dry measure parts by weight, is made up of silica 10 part, corncob 50 parts, starch 40 parts.
The preparation of above-mentioned aquatic products compound premix:
(1) preparation of seaweed bio activity extract:
Cross after 200 mesh sieves through ultramicro grinding after the raw material of above-mentioned marine alga composition is mixed, add ethanol-hydrogen chloride, carry out the collaborative extraction of ultrasonic-microwave, ultrasonic power 100W, supersonic frequency is 25kHz, Extracting temperature 45 DEG C, solid-liquid ratio 1g: 3mL, microwave power 300W, extraction time 40min, then constant volume, filter, filtrate is as extract, and extract obtains extract pulvis through freeze drying;
The distilled water (slag: water=1:10 mass volume ratio) of sterilizing is added in filter residue, stir and form marine alga slurries, the salt acid for adjusting pH value that use 4mol often rises is 5.0, add complex enzyme (cellulase again, pectase, papain, zytase is with the mixing of the ratio of mass ratio 3:2:3:2) carry out enzymolysis, enzyme concentration is the mass percent of 4% substrate, 50 DEG C of constant temperature stir enzymolysis 2 hours, its cellulase (500,000 u/g), pectase (100,000 u/ gram), papain (100,000 u/ gram), zytase (100,000 u/ gram), improve temperature to 95 DEG C, keep 15 minutes, then cool to 40 DEG C, add lactic acid bacteria (100,000,000 every milliliter) and dusty yeast (dusty yeast dry: total liquid=1:100 mass volume ratio), sealed fermenting 10-12 hour after stirring, freeze drying obtains zymotic fluid pulvis,
Be that namely 1:100 mixing obtains seaweed bio activity extract by extract pulvis and zymotic fluid pulvis in dry measure ratio.
(2) preparation of compound probiotic powder:
Probio bacterial classification is cultivated by liquid deep layer fermenting, to zymotic fluid carry out centrifugal, concentrate, add freeze drying protectant, vacuum freeze drying, obtain powdery compound probiotic powder.Measure the viable count in every gram of bacterium powder by international GB/T4789.35--2003 method, in bacterium powder, viable count is greater than 10
8cFU/g.
Wherein, freeze drying protectant composed as follows: sucrose 16g, sorbierite 16g, skimmed milk power 19g, distilled water adds to 250mL; Thalline after centrifugal concentrating and the adding proportion of freeze drying protectant are that ((g/mL), frozen-dried protective rate reaches more than 75% to 1:100.
(3) carrier is crossed 200 mesh sieves through ultramicro grinding, mix with compound acidulant, compound probiotic powder and seaweed bio activity extract, obtain compound premix.
Embodiment 2
The culture experiment of the compound premix of embodiment 1
The compound premix of embodiment 1 is added in Penaeus monodon feed as experimental group according to the ratio of mass percent 1.5%, check experiment is carried out with the Penaeus monodon feed (control group) not adding premix, wherein, the Penaeus monodon feed of control group, with air-dry thing parts by weight, each component is: fish meal 30 parts, dregs of beans 23 parts, shrimp shell meal 3 parts, peanut press pulp 10 parts, cotton dregs 4 parts, the dish dregs of rice 4 parts, wheat flour 20 parts, 1 part, fish oil, 1 part, soya-bean oil, soyabean lecithin oil 1 part, calcium dihydrogen phosphate 1 part, all the other additives 2 parts.Experimental group then adds 1.5 parts of compound premixs in the Penaeus monodon feed of control group, reduces by 1.5 portions of wheat flours.
In Weng Tian cultivation base, Wenchang, hainan city, adopt the feed of experimental group and control group to carry out contrast test, experimental group and control group be totally 10 mouthfuls of pools, 3 mu, every mouthful of pool, every mu of 50,000 tails, totally 150 ten thousand tails, the initial galassing of Penaeus monodon is 0.26 gram of every tail, raises 80 days respectively.
Result shows: the prawn survival rate 93.2% of experimental group, contrast with the Penaeus monodon (survival rate 78%) of feeding control group feed, survival rate significantly improves, the feed coefficient (1.02) of experimental group, more lower slightly than control group (1.13), the protein efficiency ratio, PER (2.23) of experimental group, higher than control group (2.06), identical number of days (80 days) the afterwards specification of the prawn of experimental group reaches average weight 15.8 grams of every tails, and control group is 14.2 grams of every tails.Feed cost due to the shrimp per ton of experimental group is lower than the feed cost of control group 100 yuan, and selling price exceeds 0.5 yuan of shrimp per jin because prawn specification is large, and the culture benefit of every mu adopting experimental group to raise earns 300 yuan than control group more.
Embodiment 3
A kind of aquatic products compound premix, with air-dry thing parts by weight, each component is: 15 parts of seaweed bio activity extracts, 10 parts of compound acidulants, 10 parts of compound probiotic powder, 75 parts of carriers.
Wherein, seaweed bio activity extract is prepared by marine alga composition and obtains, and marine alga composition is: split kettle algae 10 weight portion, sea-tangle 20 weight portion, undaria pinnitafida 10 weight portion, asparagus 35 weight portion, Enteromorpha 20 weight portion, laver 5 weight portion.
Wherein, compound probiotic powder calculates according to dry measure parts by weight, is made up of bacillus subtilis 35 parts, lactobacillus acidophilus 40 parts, clostridium butyricum 20 parts, streptococcus fecalis 5 parts.
Wherein, compound acidulant calculates according to dry measure parts by weight, sodium butyrate 55 parts, sodium Diacetate 15 parts, potassium diformate 30 parts.
Wherein, carrier calculates according to dry measure parts by weight, is made up of silica 10 part, corncob 40 parts, starch 50 parts.
The preparation of above-mentioned aquatic products compound premix:
(1) preparation of seaweed bio activity extract:
After the raw material of above-mentioned marine alga composition is mixed after ultramicro grinding, add ethanol-hydrogen chloride, carry out the collaborative extraction of ultrasonic-microwave, ultrasonic power 100W, Extracting temperature 45 DEG C, solid-liquid ratio 1g: 3mL, microwave power 400W, extraction time 40min, then constant volume, filter, filtrate is as extract, and extract obtains extract pulvis through freeze drying;
The distilled water (slag: water=1:10 mass volume ratio) of sterilizing is added in filter residue, stir and form marine alga slurries, adjust ph is 5.0, add complex enzyme (cellulase again, pectase, papain, zytase is with the mixing of the ratio of mass ratio 3:2:3:2) carry out enzymolysis, the mass percent of enzyme concentration 3% substrate, 48 DEG C of constant temperature stir enzymolysis 2.5 hours, its cellulase (500,000 u/g), pectase (100,000 u/ gram), papain (100,000 u/ gram), zytase (100,000 u/ gram), improve temperature to 95 DEG C, keep 15 minutes, then cool to 38 DEG C, add lactic acid bacteria (100,000,000 every milliliter) and dusty yeast (dry: total liquid=1:100 mass volume ratio), sealed fermenting 12 hours after stirring, freeze drying obtains zymotic fluid pulvis,
(2) preparation of compound probiotic powder:
Probio bacterial classification is cultivated by liquid deep layer fermenting, to zymotic fluid carry out centrifugal, concentrate, add freeze drying protectant, vacuum freeze drying, obtain powdery compound probiotic powder.Measure the viable count in every gram of bacterium powder by international GB/T4789.35--2003 method, in bacterium powder, viable count is greater than 10
8cFU/g.
Wherein, freeze drying protectant composed as follows: sucrose 16g, sorbierite 16g, skimmed milk power 19g, distilled water adds to 250mL; Thalline after centrifugal concentrating and the adding proportion of freeze drying protectant are that ((g/mL), frozen-dried protective rate reaches more than 75% to 1:100.
(3) by carrier through ultramicro grinding, mix with compound acidulant, compound probiotic powder and seaweed bio activity extract, obtain compound premix.
Embodiment 4
Using the compound premix of embodiment 3 according to 1% ratio add in Micropterus salmoides feed as experimental group, check experiment is carried out with the common Micropterus salmoides feed (control group) of not adding premix, wherein, the common Micropterus salmoides feed of control group, with air-dry thing parts by weight, each component is: fish meal 40 parts, dregs of beans 18 parts, tanimoto powder 5 parts, brewer's yeast 3 parts, peanut press pulp 5 parts, wheat flour 20 parts, 1.5 parts, fish oil, 1.5 parts, soya-bean oil, soyabean lecithin oil 3 parts, calcium dihydrogen phosphate 1 part, all the other additives 2 parts.Wherein, experimental group is, add 1 part of compound premix in the common Micropterus salmoides feed of above-mentioned control group after, reduce by 1 portion of wheat flour and obtain.
In Zhong Shan Tan Zhou cultivation base, Guangdong, adopt the feed of experimental group and control group to carry out contrast test, experimental group and control group be totally 8 mouthfuls of pools, and 6 mu, every mouthful of pool, every mu of 10,000 tails, totally 48 ten thousand tails, the initial galassing of Micropterus salmoides is 12 grams of every tails, respectively raising 80 days.
Result shows: the survival rate 98% of experimental group, contrast with the Micropterus salmoides (survival rate 90%) of feeding control group feed and significantly improve, the feed coefficient (1.10) of experimental group is more lower slightly than control group (1.15), identical number of days (80 days) the afterwards specification of experimental group Micropterus salmoides reaches average weight 81.5 grams of every tails, and control group is 75.0 grams of every tails.Feed cost due to the fish per ton of experimental group is lower than the feed cost of control group 70 yuan, and selling price exceeds 0.1 yuan of fish per jin because the large body colour vigor of specification is good, and the culture benefit of every mu adopting embodiment 1 to raise earns 100 yuan than control group more.It is as shown in table 1 that the growth performance of the Micropterus salmoides that experimental group and control group are fed and feed conversion and morphological indexes etc. raise result.
Table 1: the raising results contrast of experimental group and control group
Embodiment 5
A kind of aquatic products compound premix, with air-dry thing parts by weight, each component is: 12 parts of seaweed bio activity extracts, 9 parts of compound acidulants, 10 parts of compound probiotic powder, 69 parts of carriers.
Wherein, seaweed bio activity extract is prepared by marine alga composition and obtains, and marine alga composition is: split kettle algae 10 weight portion, sea-tangle 15 weight portion, undaria pinnitafida 10 weight portion, asparagus 45 weight portion, Enteromorpha 15 weight portion, laver 5 weight portion.
Wherein, compound probiotic powder calculates according to dry measure parts by weight, is made up of bacillus subtilis 45 parts, lactobacillus acidophilus 30 parts, clostridium butyricum 20 parts, streptococcus fecalis 5 parts.
Wherein, compound acidulant calculates according to dry measure parts by weight, is made up of sodium butyrate 55 parts, sodium Diacetate 20 parts, potassium diformate 25 parts.
Wherein, carrier calculates according to dry measure parts by weight, is made up of silica 10 part, corncob 45 parts, starch 45 parts.
The preparation method of this compound premix and embodiment 3 are together.
Embodiment 6
Using the compound premix of embodiment 5 according to 1.5% ratio add in Scioenops ocellatus feed as experimental group, check experiment is carried out with the common Scioenops ocellatus feed (control group) not adding premix, wherein, the general American snapper feed of control group, with air-dry thing parts by weight, each component is: fish meal 30 parts, dregs of beans 18 parts, tanimoto powder 5 parts, krill meal 5 parts, brewer's yeast 3 parts, peanut press pulp 10 parts, wheat flour 20 parts, 1.5 parts, fish oil, 1.5 parts, soya-bean oil, soyabean lecithin oil 3 parts, calcium dihydrogen phosphate 1 part, all the other additives 2 parts.Experimental group is after the general American snapper feed of control group adds 1.5 parts of compound premixs, reduces by 1.5 portions of wheat flours and obtains.
In cultivation base, Zhanjiang East Sea Island, adopt the feed of experimental group and control group to carry out contrast test, experimental group and control group be totally 5 mouthfuls of pools, and 10 mu, every mouthful of pool, every mu of 10,000 tails, totally 50 ten thousand tails, the initial galassing of Scioenops ocellatus is 9.4 grams of every tails, respectively raising 70 days.
Result shows, the survival rate 100% of experimental group, contrast with the Scioenops ocellatus (survival rate 92%) of feeding control group feed and significantly improve, feed coefficient (0.98) is more lower slightly than control group (1.06), identical number of days (70 days) the afterwards specification of the Scioenops ocellatus of experimental group reaches average weight 72.01 grams of every tails, and control group is 66.45 grams of every tails.Feed cost due to the fish per ton of experimental group is lower than the feed cost of control group 60 yuan, and selling price exceeds 0.1 yuan of fish per jin because the large body colour vigor of specification is good, and the culture benefit of every mu adopting experimental group to raise earns 90 yuan than control group more.It is as shown in table 2 that the growth performance of the Micropterus salmoides that experimental group and control group are fed and feed conversion and morphological indexes etc. raise result.
Table 2: the raising results contrast of experimental group and control group
The above; be only preferably specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and design thereof and replace or change, all should be encompassed in protection scope of the present invention.
Claims (10)
1. an aquatic products compound premix, is characterized in that, with air dry matter amount percentages, comprise 10-15% seaweed bio activity extract, the compound acidulant of 5-10%, 5-10% compound probiotic powder, all the other are carrier.
2. a kind of aquatic products compound premix as claimed in claim 1, it is characterized in that, with dry measure weight parts, described seaweed bio activity extract is obtained by following raw material: split 5 ~ 10 parts, kettle algae, 10 ~ 20 parts, sea-tangle, undaria pinnitafida 5 ~ 15 parts, asparagus 35 ~ 50 parts, Enteromorpha 15 ~ 25 parts, laver 5 ~ 15 parts.
3. a kind of aquatic products compound premix as claimed in claim 1, is characterized in that, with dry measure weight parts, described compound acidulant comprises sodium butyrate 50 ~ 70 parts, sodium Diacetate 10 ~ 20 parts, potassium diformate 20 ~ 30 parts.
4. a kind of aquatic products compound premix as claimed in claim 1, it is characterized in that, with dry measure weight parts, described compound probiotic powder comprises bacillus subtilis 30 ~ 50 parts, lactobacillus acidophilus 30 ~ 50 parts, clostridium butyricum 20 ~ 30 parts, streptococcus fecalis 5-10 part.
5. a kind of aquatic products compound premix as claimed in claim 1, is characterized in that, with dry measure weight parts, described carrier comprises silica 10 ~ 20 part, corncob 30 ~ 50 parts, starch 40 ~ 50 parts.
6. prepare a method for the aquatic products compound premix as described in claim arbitrary in claim 1-5, it is characterized in that, comprising:
(1) by the raw material ultramicro grinding of seaweed bio activity extract, ultrasonic-microwave is collaborative to be extracted and after filtering, filtrate is as extract, enzymolysis is carried out to filter residue, fermentation obtains zymotic fluid, after extract and zymotic fluid are carried out freeze drying respectively, be proportionally mixed to get seaweed bio activity extract;
(2) by carrier ultramicro grinding, then mix with compound acidulant, compound probiotic powder and seaweed bio activity extract, obtain compound premix.
7. the preparation method of a kind of aquatic products compound premix as claimed in claim 6, is characterized in that, described compound probiotic powder be through liquid fermentation and culture, centrifugal, concentrated after add freeze drying protectant, vacuum freeze drying obtains compound probiotic powder.
8. the preparation method of a kind of aquatic products compound premix as claimed in claim 6, it is characterized in that, the enzymolysis of described step (1) carries out enzymolysis for adding complex enzyme, and complex enzyme is the complex enzyme be made up of cellulase, pectase, papain, zytase.
9. the preparation method of a kind of aquatic products compound premix as claimed in claim 6, it is characterized in that, the ultrasonic-microwave of described step (1) is worked in coordination with to be extracted as in the seaweed bio activity extract raw material after ultramicro grinding and is added ethanol-hydrogen chloride, carry out the collaborative extraction of ultrasonic-microwave, described hyperacoustic power is 100W, extraction time 40min, Extracting temperature 45 DEG C, solid-liquid ratio 1g: 3mL, microwave power 250 ~ 400W.
10. the preparation method of a kind of aquatic products compound premix as claimed in claim 6, is characterized in that, the fermentation of described step (1) after enzymolysis, adds lactic acid bacteria and yeast ferments, and fermentation 10-12 hour obtains zymotic fluid.
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| CN107083347A (en) * | 2017-06-12 | 2017-08-22 | 佛山市顺德区科斯力达生物科技有限公司 | Biological agent and preparation method and application |
| CN107149053A (en) * | 2017-03-22 | 2017-09-12 | 广东粤佳饲料有限公司 | A kind of compound additive premix for promoting Penaeus monodon growth and preparation method thereof and application method |
| CN108576372A (en) * | 2017-12-29 | 2018-09-28 | 青岛百瑞吉生物工程有限公司 | A method of preparing high protein feed by raw material of cyanobacteria |
| CN109198266A (en) * | 2018-09-27 | 2019-01-15 | 清远海贝生物技术有限公司 | A kind of compound premix and its preparation method and application increasing strong fish enteron aisle |
| CN112655839A (en) * | 2020-12-14 | 2021-04-16 | 珠海市德海生物科技有限公司 | Exogenous enzyme composite preparation and preparation method and application thereof |
| WO2021074239A1 (en) * | 2019-10-15 | 2021-04-22 | Pharmabiome Ag | Use of glycerol for increasing butyrate production by bacteria in a consortium |
| TWI745896B (en) * | 2020-03-18 | 2021-11-11 | 國立台灣海洋大學 | Sea fungus extract for enhancing fish immunity and disease resistance, its preparation method and application |
| CN113749023A (en) * | 2021-10-10 | 2021-12-07 | 广东格新生物科技有限公司 | Seaweed hydrolysate produced by composite process and application thereof in shrimp culture |
| CN113796472A (en) * | 2021-05-18 | 2021-12-17 | 佳纬生物技术有限公司 | Traditional Chinese medicine algae compound feed for improving immunity of fingerling fingerlings and preparation method thereof |
| CN114470084A (en) * | 2022-01-06 | 2022-05-13 | 佛山市顺德区旺海饲料实业有限公司 | Environment-friendly and efficient composition for preventing and treating white feces of tilapia mossambica as well as preparation method and application thereof |
| CN114766593A (en) * | 2022-04-07 | 2022-07-22 | 中山大学 | Fermented seaweed essence and preparation method and application thereof |
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| CN107149053A (en) * | 2017-03-22 | 2017-09-12 | 广东粤佳饲料有限公司 | A kind of compound additive premix for promoting Penaeus monodon growth and preparation method thereof and application method |
| CN107083347A (en) * | 2017-06-12 | 2017-08-22 | 佛山市顺德区科斯力达生物科技有限公司 | Biological agent and preparation method and application |
| CN108576372A (en) * | 2017-12-29 | 2018-09-28 | 青岛百瑞吉生物工程有限公司 | A method of preparing high protein feed by raw material of cyanobacteria |
| CN109198266A (en) * | 2018-09-27 | 2019-01-15 | 清远海贝生物技术有限公司 | A kind of compound premix and its preparation method and application increasing strong fish enteron aisle |
| WO2021074239A1 (en) * | 2019-10-15 | 2021-04-22 | Pharmabiome Ag | Use of glycerol for increasing butyrate production by bacteria in a consortium |
| TWI745896B (en) * | 2020-03-18 | 2021-11-11 | 國立台灣海洋大學 | Sea fungus extract for enhancing fish immunity and disease resistance, its preparation method and application |
| CN112655839A (en) * | 2020-12-14 | 2021-04-16 | 珠海市德海生物科技有限公司 | Exogenous enzyme composite preparation and preparation method and application thereof |
| CN113796472A (en) * | 2021-05-18 | 2021-12-17 | 佳纬生物技术有限公司 | Traditional Chinese medicine algae compound feed for improving immunity of fingerling fingerlings and preparation method thereof |
| CN113749023A (en) * | 2021-10-10 | 2021-12-07 | 广东格新生物科技有限公司 | Seaweed hydrolysate produced by composite process and application thereof in shrimp culture |
| CN113749023B (en) * | 2021-10-10 | 2022-10-21 | 广东格新生物科技有限公司 | Seaweed hydrolysate produced by composite process and application thereof in shrimp culture |
| CN114470084A (en) * | 2022-01-06 | 2022-05-13 | 佛山市顺德区旺海饲料实业有限公司 | Environment-friendly and efficient composition for preventing and treating white feces of tilapia mossambica as well as preparation method and application thereof |
| CN114766593A (en) * | 2022-04-07 | 2022-07-22 | 中山大学 | Fermented seaweed essence and preparation method and application thereof |
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