CN103404729B - Composite microecologics capable of improving growth rate of stichopus japonicus and application of composite microecologics - Google Patents
Composite microecologics capable of improving growth rate of stichopus japonicus and application of composite microecologics Download PDFInfo
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Abstract
The invention provides a composite microecologics capable of improving growth rate of stichopus japonicus and application of the composite microecologics. The composite microecologics comprises bacillus subtilis, saccharomyces cerevisiae, cellulase, pectinase, xylanase, neutral protease, solid fermentation lactic acid bacteria and a carrier, wherein the bacterium content of the bacillus subtilis is greater than or equal to 1* 108 per g, the bacterium content of the saccharomyces cerevisiae is greater than or equal to 1* 109 per g, the content of the cellulase is greater than or equal to 1,000 U/g, the content of the pectinase is greater than or equal to 1,200 U/g, the content of the xylanase is greater than or equal to 1,500 U/g, the content of the neutral protease is greater than or equal to 12,000 U/g, the bacterium content of the solid fermentation lactic acid bacteria is greater than or equal to 200 mg/g, and the content of the carrier is greater than or equal to 500 mg/g. The composite microecologics can effectively improve the utilization efficiency of bait by stichopus japonicus and promote the growth of stichopus japonicus through adding zymin; the added fungicide can restrain reproduction of pathogenic bacteria in a water body and reduce the occurrence probability of diseases such as skin melting, head shaking, swollen mouth and viscus discharging; the composite microecologics has the advantages of safety, efficiency, specifity, no residue, no side effect and the like.
Description
Technical field
The invention belongs to livestock feed technical field, relate to feed addictive, be specifically related to a kind of compound micro-ecological preparation and the application thereof that improve stichopus japonicus growth rate.
Background technology
At present on apostichopus japonicus culture, great majority utilize the collocation such as algae powder, scallop powder certain proportion ooze as the feed of stichopus japonicus, and the ratio of ooze is between 20%-50%.Except ooze, in feedstuff, the vegetable raw material such as algae powder is occupied an leading position, and therefore containing more SNSP, absorbs there is certain anti-oxidant action to animal digestion.Stichopus japonicus is in reduced levels from the young to one-tenth ginseng alginase vigor always, shows that stichopus japonicus itself is rich in alignic large-scale algae digestion power to sea-tangle and undaria pinnitafida etc. more weak.The food composition of stichopus japonicus determines algin and cellulose in its feed and occupies significant proportion, but stichopus japonicus itself secretes the limited in one's ability of alginase and cellulase, and feed conversion rate is low, and growth cycle is long.Unfavorable to the growth and breeding of stichopus japonicus.
Summary of the invention
For the situation that apostichopus japonicus Selenka feed vegetable raw material composition is many and stichopus japonicus enteron aisle enzyme activity is low, the invention provides a kind of compound micro-ecological preparation and the application thereof that improve stichopus japonicus growth rate, the deficiency of stichopus japonicus enteron aisle digestive ferment is supplemented by commercial enzyme preparation and bacteria preparation, and strengthen occupy-place and the bacteria resistance function of beneficial bacterium, decrease stichopus japonicus intestines problem occurrence probability, and facilitate the growth of stichopus japonicus.
For achieving the above object, the present invention adopts following technical proposals to be achieved:
Improve a compound micro-ecological preparation for stichopus japonicus growth rate, described compound micro-ecological preparation comprises following component: the bacterial content of bacillus subtilis is not less than 1 × 10
8individual/g, the bacterial content of S. cervisiae is not less than 1 × 10
9individual/g, cellulase content is not less than 1000U/g, and pectase content is not less than 1200U/g, xylanase content is not less than 1500U/g, neutral proteinase content is not less than 12000U/g, and the bacterial content of solid fermentation lactic acid bacteria is not less than 200mg/g, and vector contg is not less than 500mg/g.
Further, described probiotics contains: the bacterial content of bacillus subtilis is 1 × 10
8individual/g; The bacterial content of S. cervisiae is 1 × 10
9individual/g; Cellulase content is 1000U/g; Pectase content is 1200U/g; Xylanase content is 1500U/g; Neutral proteinase content is 12000U/g; The bacterial content of solid fermentation lactic acid bacteria is 200mg/g, and vector contg is 500mg/g.
Further, described carrier is for adding beneficial powder, and 60 order oversizes are 0.
Present invention also offers the application of described compound micro-ecological preparation in the feed of preparation raising stichopus japonicus growth rate, the addition of described compound micro-ecological preparation is according to perfect compound feed gross weight 3 ‰-5 ‰.
Further, directly described compound micro-ecological preparation is added in stichopus japonicus daily ration perfect compound feed.
Further, described compound micro-ecological preparation and feed addictive or feedstuff are mixed and made into premix or concentrate feed form, add seawater and make pasty state, pre fermentation 12-24h obtains pasty state fermentation material, feeds to stichopus japonicus after then being mixed in the ratio of 1:2-3 with fresh ooze by pasty state fermentation material.
Compared with prior art, advantage of the present invention and technique effect are: the present invention devises a kind of novel compound micro-ecological preparation according to stichopus japonicus growth characteristics and feed composition, the complex enzymes such as the commercial neutral proteinase of concrete selection, cellulase, zytase and pectase carry out enzymolysis, the deficiency of the digested enzyme of stichopus japonicus enteron aisle can be supplemented, in order to improve the digestibility of vegetable protein, starch, effectively promote stichopus japonicus growth.
Business-like bacillus subtilis strain low temperature germination rate selected by the present invention is high, S. cervisiae reproduction speed is fast, coordinate after pre fermentation is carried out to feed with four kinds of enzyme preparations such as cellulase, pectase, zytase, neutral proteinases, ANFs in vegetable raw material can be reduced, promote the decomposition of high molecular weight protein and carbohydrate, improve the digestibility of stichopus japonicus, in conjunction with solid fermentation lactic acid bacteria, lactic acid bacteria metabolite contained by it effectively can suppress the breeding of the pathogen such as vibrios, gas pseudomonad, build healthy intestinal environment, growth promoting effects.
When embody rule, in advance pre fermentation is carried out on feed and also compensate for the low impact that enzyme preparation is played a role of breeding water body temperature, bacillus subtilis and saccharomycetic breeding can be stichopus japonicus and provide high-quality single cell protein bletilla biology antibacterial substance in the process simultaneously, decrease the occurrence probability of intestines problem.
To sum up, compound micro-ecological preparation of the present invention effectively can improve the utilization ratio of stichopus japonicus to bait by adding enzyme preparation, promote the growth of stichopus japonicus, add microbial inoculum and can suppress the breeding of the pathogenic bacteria such as Aeromonas hydrophila in water body, molten algae blood vibrios, reductionizations skin, shake the head, the mouth that swells, arrange the occurrence probability of the disease such as dirty; The advantage such as there is security, high efficiency, selectivity, noresidue compared with similar additive and have no side effect; Use described compound micro-ecological preparation to improve and maintain stichopus japonicus growth conditions, effectively improving its growth rate.
Accompanying drawing explanation
Fig. 1 joins specification to the lab diagram of enzyme preparation effect with children the interpolation time of compound micro-ecological preparation of the present invention.
Detailed description of the invention
In conjunction with following specific embodiment, the present invention is further detailed explanation in the present invention.
Embodiment 1
The compound micro-ecological preparation of raising stichopus japonicus growth rate of the present invention comprises following component: the bacterial content of bacillus subtilis is not less than 1 × 10
8individual/g, the bacterial content of S. cervisiae is not less than 1 × 10
9individual/g, cellulase content is not less than 1000U/g, and pectase content is not less than 1200U/g, xylanase content is not less than 1500U/g, neutral proteinase content is not less than 12000U/g, and the bacterial content of solid fermentation lactic acid bacteria is no less than 200mg/g, and vector contg is not less than 500mg/g.
The compound micro-ecological preparation improving stichopus japonicus growth rate described in the present embodiment comprises: bacillus subtilis 1 × 10
8individual/g, S. cervisiae 1 × 10
9individual/g, cellulase 1000U/g, pectase 1200U/g, zytase 1500U/g, neutral proteinase 12000U/g, solid fermentation lactic acid bacteria 200mg/g, carrier 500mg/g.Carrier described in the present embodiment is for adding beneficial powder, and 60 order oversizes are 0.
Compound micro-ecological preparation of the present invention improves the application in the feed of stichopus japonicus growth rate in preparation: the addition of described compound micro-ecological preparation is 3 ‰-5 ‰ of perfect compound feed gross weight, directly adds in stichopus japonicus daily ration perfect compound feed by described compound micro-ecological preparation; Or be mixed and made into premix, concentrate feed form with other feed addictive or feedstuff, add appropriate seawater, make pasty state, pre fermentation 24h obtains pasty state fermentate, then after pasty state fermentate being mixed in the ratio of 1:2-3 with fresh ooze, feed to stichopus japonicus, to improve stichopus japonicus growth rate.
The impact experiment that embodiment 2, compound micro-ecological preparation of the present invention grow stichopus japonicus
Test aquarium volume is 45L, uninterruptedly inflates during cultivation.Test is divided into 4 groups, and the 1st group is added 5g/kg enzyme preparation, and the 2nd group is added microbial inoculum 10
9cFU/kg, the 3rd group is added enzyme preparation 5g/kg+ microbial inoculum 10
9cFU/kg, the 4th group is control group, does not add enzyme preparation.Each group 6 repetitions, an aquarium is a repetition, and each aquarium puts 8 stichopus japonicus in a suitable place to breed, and specification is 9.8g ± 0.2/, and the test period is 40d.
1st group of enzyme preparation of adding is the composition removing microbial inoculum in compound micro-ecological preparation, is namely made up of the cellulase containing described content, pectase, zytase, neutral proteinase; The 2nd group of microbial inoculum added is the composition removing enzyme preparation in compound micro-ecological preparation, is namely made up of the bacillus subtilis containing described content, S. cervisiae and solid fermentation lactic acid bacteria; 3rd group is added enzyme preparation+microbial inoculum and compound micro-ecological preparation of the present invention.
As can be seen from Table 1, compound microecological group of throwing something and feeding growth rate is significantly higher than control group (P<0.05), enzyme preparation of throwing something and feeding separately or microbial inoculum, the growth rate of stichopus japonicus on average improves 8.33% than control group respectively, 9.57%(P>0.05), illustrate by cellulase, pectase, zytase, neutral proteinase composition enzyme preparation and by bacillus subtilis, S. cervisiae and the growth of solid fermentation lactic acid bacteria composition bacteria preparation to stichopus japonicus have facilitation to a certain degree, enzyme preparation and bacteria preparation composite after, effect is increased to stichopus japonicus and is better than independent participation (P>0.05).
Embodiment 3, compound micro-ecological preparation of the present invention join the impact of growth on children
Select between 2 stichopus japonicus seedling culturing vehicles, to carry out enzyme preparation and add test.Each nursery pond volume is 20 side's water, and seedling pond water temperature is between 11-13 DEG C.Compound micro-ecological preparation addition is 5g/kg feed, arranges blank group.Test period is divided into 20d and 25d, 20d group only select 4 order specifications as subjects, 25d group selects 4 orders and 6 orders, two kinds of specifications to test respectively, for comparing the interpolation time and children joins specification to the impact of enzyme preparation effect.Each test arranges 6 repetitions, on-test and at the end of weigh every pond children ginseng weight.
Experimental result as shown in Figure 1.No matter be 4 order seedlings or 6 order seedlings, continue the compound micro-ecological preparation of interpolation 5g/kg in bait after, the average growth rate of the 20-25d of the young all significantly improves (P ﹤ 0.05).20d4 order, 25d4 order, 25d6 order rate of body weight gain are respectively and improve 14.05%, 23.27% and 26.79% than control group, comparatively speaking, along with compound micro-ecological preparation adds the prolongation of time in feed, children's ginseng rate of body weight gain improves more obvious, but under identical feeding time, the test children ginseng rate of body weight gain there was no significant difference of 4 orders and 6 orders, two kinds of specifications.Before starting, just experience temperature of cultivation reduces process, and in pond, seedling has fractionated skin, swollen mouth, corn is not endured, enteron aisle is not good, epidermis is not thick, drag the phenomenon such as just.In 20-25d off-test time of falling pond, most of seedling state takes a turn for the better, and seedling look deepens green, and corn point very, formed stools, does not drag just, and the end is not smelly, swollen mouth, changes the skin seedling that is not in good state and obviously reduces.
Above embodiment only in order to technical scheme of the present invention to be described, but not is limited; Although with reference to previous embodiment to invention has been detailed description, for the person of ordinary skill of the art, still can modify to the technical scheme described in previous embodiment, or equivalent replacement is carried out to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (5)
1. improve a compound micro-ecological preparation for stichopus japonicus growth rate, it is characterized in that: described compound micro-ecological preparation is composed of the following components: the bacterial content of bacillus subtilis is not less than 1 × 10
8individual/g, the bacterial content of S. cervisiae is not less than 1 × 10
9individual/g, cellulase content is not less than 1000 U/g, and pectase content is not less than 1200 U/g, xylanase content is not less than 1500 U/g, neutral proteinase content is not less than 12000 U/g, and the bacterial content of solid fermentation lactic acid bacteria is not less than 200 mg/g, and vector contg is not less than 500 mg/g; Described carrier is for adding beneficial powder, and 60 order oversizes are 0.
2. a kind of compound micro-ecological preparation improving stichopus japonicus growth rate according to claim 1, is characterized in that: described compound micro-ecological preparation is composed of the following components: the bacterial content of bacillus subtilis is 1 × 10
8individual/g; The bacterial content of S. cervisiae is 1 × 10
9individual/g; Cellulase content is 1000 U/g; Pectase content is 1200 U/g; Xylanase content is 1500 U/g; Neutral proteinase content is 12000 U/g; The bacterial content of solid fermentation lactic acid bacteria is 200 mg/g, and vector contg is 500 mg/g.
3. compound micro-ecological preparation according to claim 1 and 2 improves the application in the feed of stichopus japonicus growth rate in preparation, it is characterized in that: the addition of described compound micro-ecological preparation is according to perfect compound feed gross weight 3 ‰-5 ‰.
4. compound micro-ecological preparation according to claim 3 improves the application in the feed of stichopus japonicus growth rate in preparation, it is characterized in that: directly added in stichopus japonicus daily ration perfect compound feed by described compound micro-ecological preparation.
5. compound micro-ecological preparation according to claim 3 improves the application in the feed of stichopus japonicus growth rate in preparation, it is characterized in that: described compound micro-ecological preparation and feed addictive or feedstuff are mixed and made into premix or concentrate feed form, add seawater and make pasty state, pre fermentation 12-24h obtains pasty state fermentation material, feeds to stichopus japonicus after then being mixed in the ratio of 1:2-3 with fresh ooze by pasty state fermentation material.
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| CN103845367A (en) * | 2014-01-03 | 2014-06-11 | 青岛东海药业有限公司 | Micro-ecological preparation applied to aquaculture of abalones and sea cucumbers |
| CN104000024A (en) * | 2014-06-06 | 2014-08-27 | 威海金牌生物科技股份有限公司 | Micro-ecological preparation for promoting growth of juvenile sea cucumbers and application method |
| CN104686838A (en) * | 2015-02-11 | 2015-06-10 | 青岛根源生物技术集团有限公司 | Composite microecological feed additive capable of increasing freshwater fish growth rate and application of composite microecological feed additive |
| CN108949615B (en) * | 2018-06-25 | 2020-05-05 | 青岛农业大学 | Microecological preparation for fermenting stichopus japonicus feed |
| CN112340856A (en) * | 2020-11-03 | 2021-02-09 | 苏州汉风科技发展有限公司 | Biological environment-friendly wastewater treatment method |
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