CN109198266A - A kind of compound premix and its preparation method and application increasing strong fish enteron aisle - Google Patents
A kind of compound premix and its preparation method and application increasing strong fish enteron aisle Download PDFInfo
- Publication number
- CN109198266A CN109198266A CN201811129938.8A CN201811129938A CN109198266A CN 109198266 A CN109198266 A CN 109198266A CN 201811129938 A CN201811129938 A CN 201811129938A CN 109198266 A CN109198266 A CN 109198266A
- Authority
- CN
- China
- Prior art keywords
- parts
- compound
- chinese medicine
- powder
- solid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000001875 compounds Chemical class 0.000 title claims abstract description 97
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title abstract description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 69
- 239000000843 powder Substances 0.000 claims abstract description 58
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 45
- 239000003814 drug Substances 0.000 claims abstract description 39
- 241000196324 Embryophyta Species 0.000 claims abstract description 37
- 239000004310 lactic acid Substances 0.000 claims abstract description 34
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 34
- 241000194017 Streptococcus Species 0.000 claims abstract description 30
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 26
- 239000000284 extract Substances 0.000 claims abstract description 24
- 241000894006 Bacteria Species 0.000 claims abstract description 23
- 239000007787 solid Substances 0.000 claims abstract description 22
- 241000605422 Asparagus asparagoides Species 0.000 claims abstract description 17
- 241000220259 Raphanus Species 0.000 claims abstract description 17
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 claims abstract description 17
- 240000004980 Rheum officinale Species 0.000 claims abstract description 17
- 235000008081 Rheum officinale Nutrition 0.000 claims abstract description 17
- 239000003833 bile salt Substances 0.000 claims abstract description 16
- 235000013312 flour Nutrition 0.000 claims abstract description 12
- 230000001580 bacterial effect Effects 0.000 claims abstract description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 13
- 238000009630 liquid culture Methods 0.000 claims description 11
- 230000007935 neutral effect Effects 0.000 claims description 11
- 229910021536 Zeolite Inorganic materials 0.000 claims description 10
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 10
- 239000010457 zeolite Substances 0.000 claims description 10
- 239000012141 concentrate Substances 0.000 claims description 9
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 8
- 238000010790 dilution Methods 0.000 claims description 8
- 239000012895 dilution Substances 0.000 claims description 8
- 239000000725 suspension Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 241000186660 Lactobacillus Species 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 239000007853 buffer solution Substances 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 2
- 241000304886 Bacilli Species 0.000 claims 2
- 239000006071 cream Substances 0.000 claims 2
- 230000000968 intestinal effect Effects 0.000 abstract description 20
- 230000007423 decrease Effects 0.000 abstract description 5
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 230000029087 digestion Effects 0.000 abstract description 4
- 210000002919 epithelial cell Anatomy 0.000 abstract description 3
- 210000004907 gland Anatomy 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000007124 immune defense Effects 0.000 abstract description 2
- 230000001771 impaired effect Effects 0.000 abstract description 2
- 210000002751 lymph Anatomy 0.000 abstract description 2
- 210000004698 lymphocyte Anatomy 0.000 abstract description 2
- 230000008439 repair process Effects 0.000 abstract description 2
- 230000004069 differentiation Effects 0.000 abstract 1
- 241000252498 Ictalurus punctatus Species 0.000 description 26
- 241000252230 Ctenopharyngodon idella Species 0.000 description 21
- 230000000694 effects Effects 0.000 description 12
- 238000012360 testing method Methods 0.000 description 11
- 239000006041 probiotic Substances 0.000 description 10
- 235000018291 probiotics Nutrition 0.000 description 10
- 210000000936 intestine Anatomy 0.000 description 9
- 239000004615 ingredient Substances 0.000 description 8
- 210000003734 kidney Anatomy 0.000 description 7
- 210000004185 liver Anatomy 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 210000000496 pancreas Anatomy 0.000 description 7
- 210000000952 spleen Anatomy 0.000 description 7
- 239000002131 composite material Substances 0.000 description 6
- 239000012676 herbal extract Substances 0.000 description 6
- 230000009467 reduction Effects 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 210000002175 goblet cell Anatomy 0.000 description 5
- 230000036541 health Effects 0.000 description 5
- 230000004888 barrier function Effects 0.000 description 4
- 230000007413 intestinal health Effects 0.000 description 4
- 210000004347 intestinal mucosa Anatomy 0.000 description 4
- 230000002195 synergetic effect Effects 0.000 description 4
- 235000005979 Citrus limon Nutrition 0.000 description 3
- 244000131522 Citrus pyriformis Species 0.000 description 3
- 230000000975 bioactive effect Effects 0.000 description 3
- 238000004821 distillation Methods 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 230000000529 probiotic effect Effects 0.000 description 3
- 230000001603 reducing effect Effects 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 210000000941 bile Anatomy 0.000 description 2
- 230000003139 buffering effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- TVTJUIAKQFIXCE-HUKYDQBMSA-N 2-amino-9-[(2R,3S,4S,5R)-4-fluoro-3-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-7-prop-2-ynyl-1H-purine-6,8-dione Chemical group NC=1NC(C=2N(C(N(C=2N=1)[C@@H]1O[C@@H]([C@H]([C@H]1O)F)CO)=O)CC#C)=O TVTJUIAKQFIXCE-HUKYDQBMSA-N 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- 208000006083 Hypokinesia Diseases 0.000 description 1
- 241001275898 Mylopharyngodon piceus Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000269799 Perca fluviatilis Species 0.000 description 1
- 241000630524 Taractes rubescens Species 0.000 description 1
- 241001251949 Xanthium sibiricum Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000027288 circadian rhythm Effects 0.000 description 1
- 230000001447 compensatory effect Effects 0.000 description 1
- 238000005314 correlation function Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000009786 epithelial differentiation Effects 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004211 gastric acid Anatomy 0.000 description 1
- 244000005709 gut microbiome Species 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000009342 intercropping Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000004798 organs belonging to the digestive system Anatomy 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000002955 secretory cell Anatomy 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 210000004876 tela submucosa Anatomy 0.000 description 1
- 210000003684 theca cell Anatomy 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/168—Steroids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/225—Faecalis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Botany (AREA)
- Mycology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of compound premixes and its preparation method and application for increasing strong fish enteron aisle, by weight, comprising: bacterium number total content >=101220~35 parts of the smelly streptococcus powder of solid-state plant source excrement of cfu, total bacterial content >=101315~30 parts of the solid lactic acid bacillus powder of cfu, 20~35 parts of compound Chinese medicine extract, 15~35 parts of flour, compound Chinese medicine extract is extracted from compound Chinese medicine and is obtained, and in parts by weight, compound Chinese medicine includes 6~12 parts of citric acid, 0.3~1.4 part of bile salt, 28~43 parts of dark plum, 20~28 parts of smilax, 15~26 parts of rheum officinale, 15~26 parts of radish seed.The beneficial effects of the present invention are: 1, peomote the abundant digestion and absorption of nutriment.2, differentiation and the cover decline epithelial cell that can activate body of gland layer epithelial cell, promote to repair impaired intestinal villus.3, the immune defense function of lymph lacteal vessel medium size lymphocyte can be enhanced.
Description
Technical field
The present invention relates to a kind of compound premix more particularly to a kind of compound premixes and its preparation for increasing strong fish enteron aisle
Methods and applications.
Background technique
Fish intestines are a kind of digestive organs of tubulose, by mucous layer, submucosa, muscle layer and theca externa in institutional framework
Composition.Functionally, fish intestines have the function of accommodating and transport, digest food and absorption nutrition, also have immune function, tool
There are physical barriers (mucus, absorption, regeneration, movement), biological barrier (normal flora symbiosis), chemical barrier (gastric acid and glycoprotein
Deng), immunization barrier (immunocompetent cell, antibody forming system etc.), be internal maximum most complicated immune organ.Therefore, enteron aisle is strong
Kang Chengdu and fish body digestive function power, physical strength and growth performance superiority and inferiority etc. are closely related.
Practice have shown that the common intestinal health problem of cultured fishes is mainly shown as three aspects, it is intestines peristalsis first
Hypodynamia leads to ingest liveness decline and excrement emptying time retardation；Followed by enteron aisle crypts body of gland layer secretory cell is living
Property decline, digestive enzymes and acidic materials secretory volume decline, influence the digestion of nutriment；Third is intestinal tissue mucous layer suede
Hair part, which is dispersed in, to fall off, and epithelial cell compensatory hypertrophy cover scarce capacity reduces nutriment and contacts with the digestion of enteron aisle
Surface area.
The performance of fish intestines problem is different, to the influence for ingesting, digest, absorbing, empty the rhythm and pace of moving things and disease induction not phases
Together.For different bowel problems, if effective intervening measure cannot be implemented, it will influence the comprehensive warp of cultured fishes
Ji benefit.
Summary of the invention
Against the above deficiency, the present invention provides compound premix of a kind of strong fish enteron aisle of increasing and preparation method thereof and answers
With.
The present invention reaches above-mentioned purpose by following scheme:
In a first aspect, a kind of compound premix for increasing strong fish enteron aisle, by weight, comprising: bacterium number total content >=
101220~35 parts of the smelly streptococcus powder of solid-state plant source excrement of cfu, total bacterial content >=1013The solid lactic acid bacillus powder 15 of cfu~
30 parts, 20~35 parts of compound Chinese medicine extract, 15~35 parts of flour, wherein the compound Chinese medicine extract is from compound Chinese medicine
Extraction obtains, and in parts by weight, the compound Chinese medicine includes 6~12 parts of citric acid, and 0.3~1.4 part of bile salt, dark plum 28~43
Part, 20~28 parts of smilax, 15~26 parts of rheum officinale, 15~26 parts of radish seed.
Preferably, the compound Chinese medicine includes 8 parts of citric acid, and 1 part of bile salt, 35 parts of dark plum, 23 parts of smilax, rheum officinale 18
Part, 15 parts of radish seed.
Preferably, a kind of fish enteron aisle increases strong compound premix, by weight, including bacterium number total content >=
101225 parts of the smelly streptococcus powder of solid-state plant source excrement of cfu, total bacterial content >=101328 parts of the solid lactic acid bacillus powder of cfu, it is compound
27 parts of Chinese medical extract, 20 parts of flour, the compound Chinese medicine includes 7~11 parts of citric acid, and 0.8~1.2 part of bile salt, dark plum 33
~36 parts, 22~25 parts of smilax, 16~19 parts of rheum officinale, 16~19 parts of radish seed.
It is further preferred that a kind of fish enteron aisle increases strong compound premix, by weight, including bacterium number always contains
Amount >=101225 parts of the smelly streptococcus powder of solid-state plant source excrement of cfu, total bacterial content >=101328 parts of the solid lactic acid bacillus powder of cfu,
27 parts of compound Chinese medicine extract, 20 parts of flour, the compound Chinese medicine includes 8 parts of citric acid, and 1 part of bile salt, 35 parts of dark plum, native Fu
23 parts of Siberian cocklebur, 18 parts of rheum officinale, 15 parts of radish seed.
Preferably, the smelly streptococcus powder of the solid-state plant source excrement is prepared by the smelly hammer strain of plant source excrement.
It is further preferred that the smelly streptococcus powder of solid-state plant source excrement is prepared by following step: by plant source
The smelly hammer strain of excrement is inoculated in lactic acid bacteria liquid culture medium, and 35 DEG C of constant-temperatureanaerobic anaerobics are fermented 48 hours, and thalline were collected by centrifugation, neutral
After PBS buffer solution dilution, thalline were collected by centrifugation, the thallus being collected into and glycerol is swayed suspension, then mix with mould proof zeolite powder,
Ensure thallus total content >=1012Cfu obtains the smelly streptococcus powder of solid-state plant source excrement.
It is further preferred that the smelly streptococcus powder of solid-state plant source excrement is prepared by following step: by plant source
The smelly hammer strain of excrement is inoculated in lactic acid bacteria liquid culture medium, and 35 DEG C of constant-temperatureanaerobic anaerobics are fermented 48 hours, and 5000rpm is centrifuged 10 minutes and receives
Collect thallus and 10 minutes collection thallus is centrifuged after neutral PBS buffer solution dilution with 5000rpm, by the thallus being collected into and 3 times of weights
The glycerol of amount volume ratio sways suspension, then mixes with mould proof zeolite powder, thallus total content >=1012Cfu obtains solid-state plant source excrement
Smelly streptococcus powder.
Preferably, the solid lactic acid bacillus powder is prepared by lactobacillus species.
It is further preferred that the solid lactic acid bacillus powder is prepared by following step: lactobacillus species are inoculated with
In lactic acid bacteria liquid culture medium of the pH less than 3.5,32 DEG C constant-temperatureanaerobic anaerobic ferment 48 hours, thalline were collected by centrifugation, neutral PBS dilution
Afterwards, thalline were collected by centrifugation, the thallus being collected into and glycerol is swayed suspension, then mix with mould proof zeolite powder, and thallus total content >=
1013Cfu obtains solid lactic acid bacillus powder.
It is further preferred that the solid lactic acid bacillus powder is prepared by following step: lactobacillus species are inoculated with
In ordinary lactic acid bacteria liquid culture medium of the pH less than 3.5,32 DEG C constant-temperatureanaerobic anaerobic ferment 48 hours, 5000rpm is centrifuged 10 minutes and collects
Thallus is centrifuged 10 minutes collection thallus after neutral PBS dilution with 5000rpm, by the thallus being collected into and 3.5 times of bulking values
The glycerol of ratio sways suspension, then mixes with mould proof zeolite powder, thallus total content >=1013Cfu obtains solid lactic acid bacillus powder.
Preferably, the lactic acid bacteria liquid culture medium includes: 11~15 parts of peptone in parts by mass, beef extract 3~5
Part, 3~5 parts of sodium chloride, 1000 parts of distilled water, adjust pH to 7.0~7.4.
Preferably, it is 7.0~7.4 that the neutral PBS buffer solution, which is pH value,.
Preferably, the compound Chinese medicine extract is prepared by following steps: being formed after compound Chinese medicine is mixed multiple
Object is closed, compound is added to the water, 90 DEG C of constant temperature decoct 25 minutes, and 40 DEG C of constant temperature decoct to the fried liquid of 3ml that be equal to 1g compound
The fried liquid of collection is concentrated, terminates to be concentrated when being equal to 1g compound with 1ml concentrate, the concentrate being collected into is multiple by object
Close Chinese medical extract.
Preferably, the compound Chinese medicine extract is prepared by following steps: being formed after compound Chinese medicine is mixed multiple
Object is closed, is sufficiently mixed after crossing 50 mesh screens, then compound is added in 2000 parts of water, 90 DEG C of constant temperature decoct 25 minutes, 40 DEG C
Constant temperature, which is decocted to the fried liquid of 3ml, is equal to 1g compound, and the fried liquid of collection is gone in rotary distillation instrument and is concentrated, dense with 1ml
Contracting liquid terminates to be concentrated when being equal to 1g compound, the concentrate bioactive compound being collected into.
Second aspect provides a kind of method for preparing above-mentioned increasing and being good for the compound premix of fish enteron aisle, comprising: plant solid-state
After the smelly streptococcus powder of material resource excrement, solid lactic acid bacillus powder, compound Chinese medicine extract, flour are sufficiently mixed, it is dried to moisture content
For 8-11%, compound premix is obtained.
Preferably, a method of preparing the compound premix that fish enteron aisle is good in above-mentioned increasing, comprising: by solid-state plant source excrement
Smelly streptococcus powder, solid lactic acid bacillus powder, compound Chinese medicine extract, flour cross 80 meshes after being sufficiently mixed, and 25 DEG C are dried to water
Dividing content is 8-11%, obtains compound premix.
Compared with prior art, the beneficial effects of the present invention are:
1, invention is rich in active bacteria, has good regulating power to fish intestines physiological function, can change
The circadian rhythm that kind or balance intestinal is wriggled peomotes the abundant digestion and absorption of nutriment.
2, the present invention is rich in plurality of Chinese extract active factors, has to harmful microorganism in intestinal microflora good
Inhibiting effect, be colonized in enteron aisle secretion extracellular products can activate body of gland layer epithelial cell differentiation and cover decline epithelium
Cell promotes to repair impaired intestinal villus.
3, the present invention, which has goblet cell activity, adjusts activation, can also enhance lymph lacteal vessel medium size lymphocyte
Immune defense function.
Specific embodiment
Below in conjunction with specific embodiment, invention is further explained.
Embodiment 1:
In parts by mass, bacterium number total content >=10 are weighed1225 parts of the smelly streptococcus powder of solid-state plant source excrement of cfu, total bacterium contains
Amount >=101328 parts of the solid lactic acid bacillus powder of cfu, 27 parts of compound Chinese medicine extract, 20 parts of flour.Then raw material components are abundant
80 mesh pulverizers are crossed after mixing and prepare mixture, and being placed in 25 DEG C and being dried to moisture content is 9%, and the mixture for collecting preparation is
Compound premix of the invention.
The smelly streptococcus powder of solid-state plant source excrement is prepared by following step: the smelly hammer strain of plant source excrement is inoculated in
Lactic acid bacteria liquid culture medium, 35 DEG C of constant-temperatureanaerobic anaerobics are fermented 48 hours, and 5000rpm is centrifuged 10 minutes collection thallus, neutral PBS buffering
After liquid dilution, 10 minutes collection thallus are centrifuged with 5000rpm, the glycerol of the thallus being collected into and 3 times of w/vs is swayed
It is suspended, then is mixed with mould proof zeolite powder, thallus total content >=1012Cfu obtains the smelly streptococcus powder of solid-state plant source excrement.
Solid lactic acid bacillus powder is prepared by following step: it is common less than 3.5 that lactobacillus species are inoculated in pH
Lactic acid bacteria liquid culture medium, 32 DEG C constant-temperatureanaerobic anaerobic ferment 48 hours, 5000rpm is centrifuged 10 minutes collections thallus, and neutral PBS dilutes
Afterwards, 10 minutes collection thallus are centrifuged with 5000rpm, the glycerol of the thallus being collected into and 3.5 times of w/vs are swayed into suspension,
It is mixed again with mould proof zeolite powder, thallus total content >=1013Cfu obtains solid lactic acid bacillus powder.
Compound Chinese medicine extract is prepared by following steps: being formed compound after compound Chinese medicine is mixed, is crossed 50 mesh
Be sufficiently mixed after sieve, then compound be added in 2000 parts of water, 90 DEG C of constant temperature decoct 25 minutes, 40 DEG C of constant temperature decoct to
The fried liquid of 3ml is equal to 1g compound, and the fried liquid of collection is gone in rotary distillation instrument and is concentrated, is equal to 1ml concentrate
Terminate to be concentrated when 1g compound, the concentrate bioactive compound being collected into, wherein compound Chinese medicine is 8 parts of citric acid, bile
1 part of salt, 35 parts of dark plum, 23 parts of smilax, 18 parts of rheum officinale, 15 parts of radish seed.
Embodiment 2
In parts by mass, bacterium number total content >=10 are weighed1228 parts of the smelly streptococcus powder of solid-state plant source excrement of cfu, total bacterium contains
Amount >=101325 parts of the solid lactic acid bacillus powder of cfu, 25 parts of compound Chinese medicine extract, 22 parts of flour.Then raw material components are abundant
80 mesh pulverizers are crossed after mixing and prepare mixture, and being placed in 25 DEG C and being dried to moisture content is 10%, collects the mixture of preparation i.e.
For compound premix of the present invention.
The smelly streptococcus powder of solid-state plant source excrement is prepared by following step: the smelly hammer strain of plant source excrement is inoculated in
Lactic acid bacteria liquid culture medium, 35 DEG C of constant-temperatureanaerobic anaerobics are fermented 48 hours, and 5000rpm is centrifuged 10 minutes collection thallus, neutral PBS buffering
After liquid dilution, 10 minutes collection thallus are centrifuged with 5000rpm, the glycerol of the thallus being collected into and 3 times of w/vs is swayed
It is suspended, then is mixed with mould proof zeolite powder, thallus total content >=1012Cfu obtains the smelly streptococcus powder of solid-state plant source excrement.
Solid lactic acid bacillus powder is prepared by following step: it is common less than 3.5 that lactobacillus species are inoculated in pH
Lactic acid bacteria liquid culture medium, 32 DEG C constant-temperatureanaerobic anaerobic ferment 48 hours, 5000rpm is centrifuged 10 minutes collections thallus, and neutral PBS dilutes
Afterwards, 10 minutes collection thallus are centrifuged with 5000rpm, the glycerol of the thallus being collected into and 3.5 times of w/vs are swayed into suspension,
It is mixed again with mould proof zeolite powder, thallus total content >=1013Cfu obtains solid lactic acid bacillus powder.
Compound Chinese medicine extract is prepared by following steps: being formed compound after compound Chinese medicine is mixed, is crossed 50 mesh
Be sufficiently mixed after sieve, then compound be added in 2000 parts of water, 90 DEG C of constant temperature decoct 25 minutes, 40 DEG C of constant temperature decoct to
The fried liquid of 3ml is equal to 1g compound, and the fried liquid of collection is gone in rotary distillation instrument and is concentrated, is equal to 1ml concentrate
Terminate to be concentrated when 1g compound, the concentrate bioactive compound being collected into, wherein compound Chinese medicine is 8 parts of citric acid, bile
1 part of salt, 35 parts of dark plum, 23 parts of smilax, 18 parts of rheum officinale, 15 parts of radish seed.
Test example 1
12 ponds are chosen from the Hubei Channel-catfish fish culture field Wu Han, initially putting in a suitable place to breed for each pond pool Channel-catfish fish and intercropping fish is close
It spends identical.9 ponds are randomly divided into totally 4 groups of I, II, III, IV, every group sets 3 in parallel, and wherein I group is fed makes containing embodiment 1
The commercial feed (abbreviation I#) of standby compound premix, II group feed the commercial feed of the compound premix prepared containing embodiment 2
(abbreviation II#), III group feeds Channel-catfish fish commercial feed (abbreviation III#) of the same brand with nutrition class, as blank control group, IV
Group feeds the commercial feed for the compound premix that composite Chinese herbal extract lacks in embodiment 1, as test control group.Test
Period 6 months.Pond is grouped and its feeds information and is as follows:
In test period, taken at random from each pond within the 30th day, 60 days, 90 days, 120 days, 150 days, 180 days in test respectively
15 tail of sample, then it is long to Ding Channel-catfish fish enteron aisle bearing capacity, enteron aisle goblet cell quantity, intestinal wall thickness, intestinal mucosa thickness and intestinal villus by Ce
Degree carries out group difference analysis to each evaluation index according to statistical method.Wherein enteron aisle bearing capacity calculates as follows,
Other indexs are counted by IPP6.0 image processing software.
When testing the 180th day, each pond feedstuff feeding total amount is counted, seine, which fish with a net, from pond have a net increase of resurveying
It calculates, while dissecting Cai sample Ce to Ding Channel-catfish fish liver body of pancreas ratio, spleen body ratio and kidney body ratio index, These parameters calculation formula as follows:
The above results measurement is as shown in Table 1 and Table 2.
Table 1:
As shown in table 1, compared with to according to group Channel-catfish fish, compound premix prepared by the present invention is added in Channel-catfish fish commercial feed
Material, it is long continuously to feed Dui Channel-catfish fish enteron aisle bearing capacity, intestinal villus goblet cell quantity, intestinal wall thickness, intestinal mucosa thickness and intestinal villus
Degree is obviously improved (p < 0.05).Thus illustrate, using compound premix provided by the invention, have to Zeng Qiang Channel-catfish fish intestinal health
There is significant effect.
On the other hand, in the embodiment of the present invention, when composite Chinese herbal extract lacks, the intestines of this invention Shi Shi Li Channel-catfish fish
Though road bearing capacity, intestinal villus goblet cell quantity, intestinal wall thickness, intestinal mucosa thickness and intestinal villus length have a degree of mention
It rises, but the complete Shi Yan Channel-catfish fish group of the present embodiment prescription is still not achieved in the expression of its effect.It can be seen that in the embodiment of the present invention,
Composite Chinese herbal extract forms compound prescription to probiotics a variety of in the present embodiment, by recommending after time use to the intestines of Channel-catfish fish
There is apparent cooperate with to be good for intestines synergistic effect in road.
Table 2:
As shown in table 2, compared with to according to group Channel-catfish fish, compound premix prepared by the present invention is added in Channel-catfish fish commercial feed
Material, continuously feed can Xian write Jiang Di Channel-catfish fish bait coefficient (p < 0.05), Xian writes Ti Gao Channel-catfish fish liver body of pancreas ratio, spleen body ratio and kidney body ratio
Index (p < 0.05).Thus illustrate, using compound premix provided by the invention, You is conducive to Jiang Di Channel-catfish fish stage feed coefficient,
Liver body of pancreas ratio, spleen body ratio and kidney body ratio index are improved simultaneously.
On the other hand, in the embodiment of the present invention, when composite Chinese herbal extract lacks, the bait of this invention Shi Shi Li Channel-catfish fish
Expect that the complete inventive embodiments Zu Channel-catfish fish of coefficient ratio is high, and its liver body of pancreas ratio, spleen body ratio, kidney body ratio are also than completely inventing implementation
Li Zu Channel-catfish fish is low.
It can be seen that composite Chinese herbal extract forms probiotics a variety of in the present embodiment compound in inventive embodiments
Prescription can be promoted in the present embodiment prescription in terms of a variety of probiotics Jiang Di Channel-catfish fish bait coefficients after recommending time use
Effect, while Ti Gao Channel-catfish fish liver body of pancreas ratio, spleen body ratio, kidney body ratio index.
In summary measurement result not only refers to intestinal health key it is found that using compound premix provided by the invention
Significant effect is indicated, also has significant effect to reducing feed coefficient and improving dirty body index, is presented as increasing in effect performance
Qiang Channel-catfish fish intestinal health, while Bu Ying Xiang Channel-catfish fish growth performance key index.
Test example 2
In Fanyu sea-gull island proving ground, indoor Shui nationality cage breeding is grown, and water quality meets DB51/T 2489-2018 requirement, is set altogether
11 test groups, every group is divided into three parallel, each parallel 100 tails of grass carp.Test period 180 days, test grass carp was averagely advised
80 ± 3 grams of lattice.The commercial feed that wherein A group is fed contains the compound premix (abbreviation premix) of the preparation of embodiment 1, B to L group
There is the independent missing of premix component in the feed fed respectively, M group feeds same brand and raises with nutrition class Channel-catfish fish monger's product
Material, is set as blank control group.As a result as shown in table 3, table 4 and table 5.
Table 3:
As shown in table 3, when the component contained by premix individually lacks, ingredient deletion experiment group grass carp bait coefficient ratio
Premix group grass carp feed coefficient significantly rises (p < 0.05), this explanation, when the single ingredient in premix group lacks, premix
Effect is obviously not as good as full constituent premix in terms of to feed of grass carp feed coefficient.The smelly streptococcus of plant source excrement contained by the premix
When all lacking with Bacillus acidi lactici, it that is to say that premix only exists citric acid, bile salt, dark plum, smilax, rheum officinale, radish
When subconstiuent, compared to the control group, the addition use of premix has certain reducing effect to grass carp feed coefficient；Contained by premix
When the smelly streptococcus of plant source excrement and Bacillus acidi lactici individually lack, it that is to say that premix only exists the one of which of probiotics, lemon
When lemon acid, bile salt, dark plum, smilax, rheum officinale, radish subconstiuent, compared to the control group, the addition use of premix is to grass carp bait
Expect that coefficient is reduced without positive effect.It is all with probiotics compared to the control group when the non-probiotic ingredient contained by the premix individually lacks
Complete group, the addition use of premix has certain reducing effect to grass carp feed coefficient.It can be seen that the smelly streptococcus of plant source excrement and
Lactic acid bar and citric acid, bile salt, dark plum, smilax, rheum officinale, radish seed cooperate with increasing with having in terms of grass carp feed coefficient
Effect acts on, and any effective component is lacked in premix, and will cause premix reduces functional effect to grass carp feed coefficient
The reduction of aspect.
Table 4:
Meanwhile as known from Table 4, it when the dark plum, smilax and bile salt that contain in premix individually lack, can make respectively
At Grass Carp Liver body of pancreas than, spleen body than the reduction with kidney body ratio.Therefore the missing of the individual key components of premix, will cause
Premix the reduction in terms of related function immune to grass carp.
Table 5:
As shown in table 5, when the component contained by premix individually lacks, ingredient deletion experiment group grass carp bait coefficient ratio
Five premix group grass carp intestinal bearing capacity, goblet cell quantity, intestinal wall thickness, intestinal mucosa thickness, intestinal villus length indexs are all
It significantly reduces (p < 0.05), this explanation, when the single ingredient in premix group lacks, premix is to grass carp intestinal tissue health
The effect of index of correlation is obviously not as good as full constituent premix.The smelly streptococcus of plant source excrement and Bacillus acidi lactici contained by the premix are whole
When missing, when that is to say that premix only exists citric acid, bile salt, dark plum, smilax, rheum officinale, radish subconstiuent, compared to pair
According to group, the addition use of premix has certain reduction to influence grass carp intestinal tissue health index of correlation；It plants contained by the premix
When the smelly streptococcus of material resource excrement and Bacillus acidi lactici individually lack, it that is to say that premix only exists the one of which of probiotics, lemon
When acid, bile salt, dark plum, smilax, rheum officinale, radish subconstiuent, compared to the control group, ingredient lacks the addition use pair of premix
Grass carp intestinal tissue health index of correlation is without reinforcing effect.When the non-probiotic ingredient contained by the premix individually lacks, compared to pair
According to group and various group of probiotics, the addition use of ingredient missing premix has grass carp intestinal tissue health index of correlation certain weak
Changing influences.It can be seen that the smelly streptococcus of plant source excrement and lactic acid bar and citric acid, bile salt, dark plum, smilax, rheum officinale, radish
Son lacks any effective component, can make with having synergistic function in terms of grass carp feed coefficient in premix
At premix to the reduction in terms of grass carp intestinal tissue health index of correlation function.
In the present invention, composite Chinese herbal extract makes as one of important prescription of the invention with a variety of probiotic combinations
With after pressing recommendation time use, to Channel-catfish fish with significantly strong intestines synergistic function, to reduction feed coefficient, raising liver pancreas
Body ratio, spleen body ratio and kidney body are than also having synergistic function.
The present invention, which passes through on the breed varieties such as grass carp, black carp, perch, golden silvery pomfret to add, to be used, and the test data of acquisition is equal
Prove that the present invention there is significant enteron aisle to increase strong effect fish.
The above, preferable specific embodiment only of the invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Design is subject to equivalent substitution or change, should be covered by the scope of protection of the present invention.
Claims (10)
1. a kind of compound premix for increasing strong fish enteron aisle, which is characterized in that by weight, comprising: bacterium number total content >=
101220~35 parts of the smelly streptococcus powder of solid-state plant source excrement of cfu, total bacterial content >=1013The solid lactic acid bacillus powder 15 of cfu~
30 parts, 20~35 parts of compound Chinese medicine extract, 15~35 parts of flour, wherein the compound Chinese medicine extract is from compound Chinese medicine
Extraction obtains, and in parts by weight, the compound Chinese medicine includes 6~12 parts of citric acid, and 0.3~1.4 part of bile salt, dark plum 28~43
Part, 20~28 parts of smilax, 15~26 parts of rheum officinale, 15~26 parts of radish seed.
2. a kind of compound premix for increasing strong fish enteron aisle according to claim 1, which is characterized in that the compound Chinese medicine
Including 8 parts of citric acid, 1 part of bile salt, 35 parts of dark plum, 23 parts of smilax, 18 parts of rheum officinale, 15 parts of radish seed.
3. a kind of compound premix for increasing strong fish enteron aisle according to claim 1, which is characterized in that by weight,
Including bacterium number total content >=101225 parts of the smelly streptococcus powder of solid-state plant source excrement of cfu, total bacterial content >=1013The solid-state cream of cfu
28 parts of acidfast bacilli powder, 27 parts of compound Chinese medicine extract, 20 parts of flour, wherein the compound Chinese medicine includes 7~11 parts of citric acid,
0.8~1.2 part of bile salt, 33~36 parts of dark plum, 22~25 parts of smilax, 16~19 parts of rheum officinale, 16~19 parts of radish seed.
4. a kind of compound premix for increasing strong fish enteron aisle according to claim 1, which is characterized in that by weight,
Including bacterium number total content >=101225 parts of the smelly streptococcus powder of solid-state plant source excrement of cfu, total bacterial content >=1013The solid-state cream of cfu
28 parts of acidfast bacilli powder, 27 parts of compound Chinese medicine extract, 20 parts of flour, the compound Chinese medicine includes 8 parts of citric acid, and 1 part of bile salt,
35 parts of dark plum, 23 parts of smilax, 18 parts of rheum officinale, 15 parts of radish seed.
5. the compound premix of fish enteron aisle is good for any a kind of increasing in 4 according to claim 1, which is characterized in that institute
The smelly streptococcus powder of solid-state plant source excrement is stated to be prepared by the smelly hammer strain of plant source excrement.
6. a kind of compound premix for increasing strong fish enteron aisle according to claim 5, which is characterized in that the solid-state plant
Excrement smelly streptococcus powder in source is prepared by following step: the smelly hammer strain of plant source excrement is inoculated in lactic acid bacteria liquid culture
Base, 35 DEG C of constant-temperatureanaerobic anaerobics are fermented 48 hours, and thalline were collected by centrifugation, and after neutral PBS buffer solution dilution, thalline were collected by centrifugation, will receive
The thallus and glycerol collected sways suspension, then mixes with mould proof zeolite powder, it is ensured that thallus total content >=1012Cfu obtains solid-state plant
The smelly streptococcus powder of material resource excrement.
7. the compound premix of fish enteron aisle is good for any a kind of increasing in 4 according to claim 1, which is characterized in that institute
Solid lactic acid bacillus powder is stated to be prepared by lactobacillus species.
8. a kind of compound premix for increasing strong fish enteron aisle according to claim 7, which is characterized in that the solid lactic acid
Bacillus powder is prepared by following step: lactobacillus species are inoculated in lactic acid bacteria liquid culture medium of the pH less than 3.5,32 DEG C
Constant-temperatureanaerobic anaerobic ferment 48 hours, thalline were collected by centrifugation, and after neutral PBS dilution, thalline were collected by centrifugation, by the thallus being collected into and glycerol
Suspension is swayed, then is mixed with mould proof zeolite powder, thallus total content >=1013Cfu obtains solid lactic acid bacillus powder.
9. the compound premix of fish enteron aisle is good for any a kind of increasing in 4 according to claim 1, which is characterized in that institute
It states compound Chinese medicine extract to be prepared by following steps: forming compound after compound Chinese medicine is mixed, compound is added
In water, 90 DEG C of constant temperature are decocted 25 minutes, and 40 DEG C of constant temperature, which are decocted to the fried liquid of 3ml, is equal to 1g compound, by the fried liquid of collection
Concentration, terminates to be concentrated when being equal to 1g compound with 1ml concentrate, and the concentrate being collected into is compound Chinese medicine extract.
10. a kind of method for preparing the compound premix of any strong fish enteron aisle of the increasing in claim 1 to 9, feature
It is, comprising: be sufficiently mixed the smelly streptococcus powder of solid-state plant source excrement, solid lactic acid bacillus powder, compound Chinese medicine extract, flour
Afterwards, being dried to moisture content is 8-11%, obtains compound premix.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811129938.8A CN109198266A (en) | 2018-09-27 | 2018-09-27 | A kind of compound premix and its preparation method and application increasing strong fish enteron aisle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811129938.8A CN109198266A (en) | 2018-09-27 | 2018-09-27 | A kind of compound premix and its preparation method and application increasing strong fish enteron aisle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109198266A true CN109198266A (en) | 2019-01-15 |
Family
ID=64981650
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811129938.8A Pending CN109198266A (en) | 2018-09-27 | 2018-09-27 | A kind of compound premix and its preparation method and application increasing strong fish enteron aisle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109198266A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640871A (en) * | 2012-04-27 | 2012-08-22 | 杨洪星 | Feed additive for aquatic livestock |
| CN102696980A (en) * | 2012-05-23 | 2012-10-03 | 广州市海维饲料有限公司 | Microecologics for preventing fatty liver of grass carp and preparation method thereof |
| CN104431621A (en) * | 2014-12-23 | 2015-03-25 | 广东海大集团股份有限公司 | Compound premix for aquatic products and preparation method of compound premix |
| CN105685529A (en) * | 2016-03-03 | 2016-06-22 | 广东海因特生物技术集团有限公司 | Feed additive for preventing tilapia enteritis |
| CN106954761A (en) * | 2017-03-30 | 2017-07-18 | 广州市海维饲料有限公司 | A kind of ecological feed for repairing grass carp intestinal villus and preparation method thereof |
-
2018
- 2018-09-27 CN CN201811129938.8A patent/CN109198266A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640871A (en) * | 2012-04-27 | 2012-08-22 | 杨洪星 | Feed additive for aquatic livestock |
| CN102696980A (en) * | 2012-05-23 | 2012-10-03 | 广州市海维饲料有限公司 | Microecologics for preventing fatty liver of grass carp and preparation method thereof |
| CN104431621A (en) * | 2014-12-23 | 2015-03-25 | 广东海大集团股份有限公司 | Compound premix for aquatic products and preparation method of compound premix |
| CN105685529A (en) * | 2016-03-03 | 2016-06-22 | 广东海因特生物技术集团有限公司 | Feed additive for preventing tilapia enteritis |
| CN106954761A (en) * | 2017-03-30 | 2017-07-18 | 广州市海维饲料有限公司 | A kind of ecological feed for repairing grass carp intestinal villus and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110835616B (en) | Active substance of lactobacillus paracasei GKS6, composition containing same and application of active substance in promoting longevity | |
| CN102041238B (en) | Lactobacillus plantarum, method for fermenting and preparing bacteriocin of Lactobacillus plantarum, and application of Lactobacillus plantarum and bacteriocin | |
| CN104762224B (en) | For improving the bacterial strain and method of ruminant health and/or performance | |
| US10130080B2 (en) | Obesity animal model and methods for making and using thereof | |
| CN104774776B (en) | The Bu Ladi saccharomycete of one plant of high-temp resisting high-humidity resisting and its application | |
| CN107164263A (en) | A kind of adjustable function of intestinal canal, the lactobacillus fermenti HY01 for preventing colitis and application thereof | |
| CN110892990B (en) | Probiotic and prebiotic edible composition and application thereof | |
| CN105985918A (en) | Anti-obesity lactic acid bacteria strain and application thereof in food composition and medical composition | |
| CN109259146A (en) | One plant has effects that inhibit the Lactobacillus rhamnosus of fatty live lesions and its application | |
| CN105420150A (en) | Lactobacillus acidophilus and application thereof | |
| CN110833565B (en) | Active substance of lactobacillus plantarum GKM3, composition containing same and application of active substance in promoting longevity | |
| CN106795482A (en) | Allow the lactobacillus composition of the juvenile growth of promotion humans and animals in the case of malnutrition | |
| CN102293349A (en) | Composite probiotic meat chicken feed additive and application thereof | |
| CN110835615B (en) | Active substance of bifidobacterium lactis GKK2, composition containing same and application of active substance and composition in promoting longevity | |
| CN111387383A (en) | High-polyphenol-content probiotic fermented apple juice with weight-losing and lipid-lowering effects and preparation method thereof | |
| CN108373983A (en) | Pediococcus pentosaceus CCFM1012, its fermented food and its application in preparing antagonism C. jejuni infec-tion drug | |
| KR102177535B1 (en) | Culture medium composition for lactobacillus using dry shitake mushroom extract | |
| CN109198266A (en) | A kind of compound premix and its preparation method and application increasing strong fish enteron aisle | |
| CN105039221B (en) | One plant of cold water fish probiotics Arthrobacter strain and application thereof | |
| CN102702373A (en) | Whole peptidoglycan of lactobacillus cell wall and application in improving IEC (intestinal epithelial cell) antimicrobial peptide expression level thereof | |
| CN112825976A (en) | Method for improving egg quality of laying hens by using peony seed meal and application | |
| CN107418905A (en) | One plant of cold water fish probiotic lactic acid lactococcus strain and application thereof | |
| CN105624070A (en) | Lactobacillus rhamnosus W27 capable of improving eggshell strength and laying capacity of laying hens and application of lactobacillus rhamnosus W27 | |
| CN117736941B (en) | Bacillus licheniformis King58 beneficial to canine bones and application thereof | |
| Moustafa et al. | Nutritional efficiency and economic traits of silkworm Bombyx mori, L. reared on mulberry leaves fortified with synbiotics |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190115 |