CN105685529A - Feed additive for preventing tilapia enteritis - Google Patents
Feed additive for preventing tilapia enteritis Download PDFInfo
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- CN105685529A CN105685529A CN201610121052.3A CN201610121052A CN105685529A CN 105685529 A CN105685529 A CN 105685529A CN 201610121052 A CN201610121052 A CN 201610121052A CN 105685529 A CN105685529 A CN 105685529A
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- tilapia
- enteritis
- feed additive
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- bacillus licheniformis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a feed additive for preventing tilapia enteritis. The feed additive comprises citric acid, bile salt, zeolite powder, sodium chloride, glucose, lactic streptococci, lactic acid bacillus and bacillus licheniformis. By improving the thickness of intestinal mucosae, the intestinal nonspecific physical defense function is enhanced, reproduction of intestinal harmful microorganisms is inhibited, release of harmful substances is promoted, the capacity of fish bodies for resisting enteritis caused by invasion of extraneous pathogenic harmful organisms is improved, and therefore the tilapia enteritis and death caused by the tilapia enteritis are prevented.
Description
[technical field]
The present invention relates to a kind of fish feed additive, particularly relate to a kind of feed additive preventing tilapia enteritis, belong to feeding additive aquatic animal field。
[background technology]
Tilapia is a kind of extensive to cultivate in the economic fish of South China of China, and annual production about reaches 1,500,000 tons, to pulling China's aquatic products domestic demand and driving product water outlet to have significant contribution。Tilapia intestinal is the important place absorbing nutrient, and intestinal tissue is made up of mucous layer, tela submucosa, muscle layer and theca externa。Wherein mucous layer includes intestinal villus, crypts composition, has both had the Absorption to nutrient substance, it may have special secretory function, particularly regulate metabolism and maintain body internal and external environment relative stably etc. in play an important role。
Practice have shown that, in breeding process, by the double influence of environmental factors and fish body intestinal tissue physiologic factor, bacterial enteritis disease has become the second largest factor threatening tilapia cultivation after streptococcicosis；Time serious, the ill tilapia state of an illness spreads rapidly, and mortality rate is significantly high, often results in serious culturing economic loss。
[summary of the invention]
For above-mentioned problems of the prior art, it is an object of the invention to provide a kind of feed additive preventing tilapia enteritis, effectively reduce the incidence rate of enteritis in tilapia breeding process。
To achieve these goals, the present invention is by the following technical solutions:
A kind of feed additive preventing tilapia enteritis, by weight percentage, including citric acid 10-15%, bile salts 1-3%, zeolite powder 1-4%, sodium chloride 0.1-0.3%, glucose 0.5-2%, also including streptococcus acidi lactici, lactobacillus, Bacillus licheniformis, wherein, the content of described streptococcus acidi lactici is 1013Individual/more than g, lactobacillus content is 1013Individual/more than g, Bacillus licheniformis content is 108Individual/more than g, all the other compositions are maize cob meal。
Preferably, the feed additive of above-mentioned prevention tilapia enteritis, by weight percentage, including citric acid 12%, bile salts 2%, zeolite powder 2%, sodium chloride 0.2%, glucose 1%, also include streptococcus acidi lactici, lactobacillus, Bacillus licheniformis, wherein, the content of described streptococcus acidi lactici is 1 × 1014Individual/g, lactobacillus content is 1 × 1014Individual/g, Bacillus licheniformis content is 1 × 109Individual/g, all the other compositions are maize cob meal。
Described streptococcus acidi lactici, lactobacillus and Bacillus licheniformis are all preferably isolated from tilapia intestinal contents flora。
The feed additive of above-mentioned prevention tilapia enteritis adding proportion in tilapia feed is preferably 1-3%。
Compared with prior art, there is advantages that
1, feed additive of the present invention includes streptococcus acidi lactici, lactobacillus and Bacillus licheniformis, and three kinds of probioticss are used in combination and can effectively activate gut-active, strengthens intestinal secretion and digestive function, it is possible to the effective breeding suppressing harmful intestinal tract microorganism species。
2, feed additive of the present invention includes citric acid, bile salts, zeolite powder isoreactivity material, not only improve the physical barriers function strengthening intestinal mucosa, is also beneficial to absorption and removes harmful intestinal tract material, safeguarding intestinal health。
3, streptococcus acidi lactici, lactobacillus and Bacillus licheniformis are isolatable from tilapia intestinal contents flora, it is more beneficial for field planting in digestive tract after being ingested, growth and secretes useful extracellular products, it is to avoid showing the probability to tilapia pathogenic effects because growing environment is uncomfortable。
4, feed additive of the present invention is in production practices, by improving intestinal mucosa thickness to strengthen the non-specific physics defense function of intestinal, suppress the breeding of harmful intestinal tract microorganism and promote the discharge of harmful substance, improve fish body and resist the ability of exogenous pathogen pest invasion initiation enteritis, thus preventing the generation of tilapia enteritis and the death of initiation。
[detailed description of the invention]
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this。When not specified (NS), reagent, equipment and the method that the present invention adopts is the conventional commercial reagent of the art, equipment and conventional use of method。
Embodiment 1
A kind of feed additive preventing tilapia enteritis, by weight percentage, including citric acid 10%, bile salts 3%, zeolite powder 4%, sodium chloride 0.1%, glucose 0.5%, also including streptococcus acidi lactici, lactobacillus, Bacillus licheniformis, wherein, the content of described streptococcus acidi lactici is 1 × 1013Individual/g, lactobacillus content is 1 × 1013Individual/g, Bacillus licheniformis content is 1 × 108Individual/g, all the other compositions are maize cob meal。In the present embodiment, described streptococcus acidi lactici, lactobacillus and Bacillus licheniformis are all isolatable from tilapia intestinal contents flora。
Utilize conventional method, proportionally mix after above-mentioned raw materials is pulverized, the feed additive of tilapia enteritis must be prevented。
Embodiment 2
A kind of feed additive preventing tilapia enteritis, by weight percentage, including citric acid 12%, bile salts 2%, zeolite powder 2%, sodium chloride 0.2%, glucose 1%, also including streptococcus acidi lactici, lactobacillus, Bacillus licheniformis, wherein, the content of described streptococcus acidi lactici is 1 × 1014Individual/g, lactobacillus content is 1 × 1014Individual/g, Bacillus licheniformis content is 1 × 109Individual/g, all the other compositions are maize cob meal。In the present embodiment, described streptococcus acidi lactici, lactobacillus and Bacillus licheniformis are all isolatable from tilapia intestinal contents flora。
Utilize conventional method, proportionally mix after above-mentioned raw materials is pulverized, the feed additive of tilapia enteritis must be prevented。
Embodiment 3
A kind of feed additive preventing tilapia enteritis, by weight percentage, including citric acid 15%, bile salts 1%, zeolite powder 1%, sodium chloride 0.3%, glucose 2%, also including streptococcus acidi lactici, lactobacillus, Bacillus licheniformis, wherein, the content of described streptococcus acidi lactici is 1 × 1015Individual/g, lactobacillus content is 1 × 1015Individual/g, Bacillus licheniformis content is 1 × 1010Individual/g, all the other compositions are maize cob meal。In the present embodiment, described streptococcus acidi lactici, lactobacillus and Bacillus licheniformis are all isolatable from tilapia intestinal contents flora。
Utilize conventional method, proportionally mix after above-mentioned raw materials is pulverized, the feed additive of tilapia enteritis must be prevented。
Compliance test result culture experiment:
Tilapia feed commercially is divided into 4 parts, it is calculated as test group 1, test group 2, test group 3 and matched group respectively, wherein, test group 1, test group 2, test group 3 add the feed additive that embodiment 1, embodiment 2 and embodiment 3 prepare respectively, its adding proportion respectively 1%, 2%, 3%, matched group feedstuff is without any allogenic material。
Choosing the healthy tilapia that initial weight is about 150g, be randomly divided into 4 groups, often group arranges 3 repetitions, and each repetition has tilapia 100 tail, puts in a suitable place to breed in still-water pond net cage。
During test, four groups of tilapias are thrown something and fed the feedstuff of test group 1, test group 2, test group 3 and matched group respectively, throw something and feed every day three times, Feeding time is 8:00,14:00,18:00 respectively, and the test period is 30 days, and period temperature range is 28-35 DEG C, water pH value 7.5-7.9, dissolved oxygen >=8.0mg/L, ammonia nitrogen≤0.1mg/L, nitrite≤0.05mg/L。
Intestinal mucosa histomorphometric analysis: respectively when on-test and end, 5 tail tilapias are fished at random from each group, solution takes its intestinal tissue, it is fixed in 10% in emerging formalin solution, the microsection manufacture of each intestinal tissue is completed through steps such as serial dehydration, dyeing, transparent, fixing, mountings, adding up the average mucosa thickness of each group of tilapia, result is as shown in table 1:
The intestinal mucosa thickness of tilapia respectively organized by table 1
As shown in Table 1, the pure feedstuff of matched group of throwing something and feeding 30 days, tilapia intestinal mucosa thickness keeps physiological to develop。And the test group feedstuff of the feed additive adding embodiment 1, embodiment 2 and embodiment 3 preparation of throwing something and feeding is after 30 days, there is extremely significant difference in tilapia intestinal mucosa thickness, and wherein tilapia anterior intestine, middle intestinal and hindgut mucosal thickness are significantly thicker than matched group (P < 0.01)。This illustrates, adds the feed additive of embodiment 1, embodiment 2 and embodiment 3 preparation, it is possible to the significant mucosal thickness strengthening tilapia intestinal in tilapia feed。
Artificial viable bacteria infection experiment: test carry out 30 days after, by escherichia coli viable bacteria amplification be diluted to 1.5 × 105cfu/ml, by mix raise oral in the way of according to every tail tilapia single to bacterium solution amount 0.2mL。It is administered orally in 96 hours containing escherichia coli viable bacteria feed tilapia, records each group of tilapia morbidity, dead situation, as shown in table 2:
Tilapia Experimental infection result respectively organized by table 2
As shown in table 2, throw something and feed continuously the feedstuff 30 days of the feed additive that with the addition of embodiment 1, embodiment 2 and embodiment 3 preparation, the enteritis that the escherichia coli living bacterial liquid that per os is given by tilapia causes has significant preventive effect, the mortality rate of three test group tilapias only respectively 5.6%, 5.1% and 6.2%, and after matched group peroral infection escherichia coli, the tilapia of 75% is lethal because of infection-induced hemorrhagic enteritis。This illustrates, adds the feed additive described in inventive formulation in feedstuff, it is possible to the enteritis or lethal that effective prevention escherichia coli peroral infection causes。
The above; it is only the preferably specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; any those familiar with the art is in the technical scope that the invention discloses; it is equal to replacement according to technical scheme and design thereof or is changed, all should be encompassed in protection scope of the present invention。
Claims (5)
1. the feed additive preventing tilapia enteritis, it is characterized in that, by weight percentage, including citric acid 10-15%, bile salts 1-3%, zeolite powder 1-4%, sodium chloride 0.1-0.3%, glucose 0.5-2%, also include streptococcus acidi lactici, lactobacillus, Bacillus licheniformis, wherein, the content of described streptococcus acidi lactici is 1013Individual/more than g, lactobacillus content is 1013Individual/more than g, Bacillus licheniformis content is 108Individual/more than g, all the other compositions are maize cob meal。
2. the feed additive of prevention tilapia enteritis according to claim 1, it is characterized in that, by weight percentage, including citric acid 12%, bile salts 2%, zeolite powder 2%, sodium chloride 0.2%, glucose 1%, also include streptococcus acidi lactici, lactobacillus, Bacillus licheniformis, wherein, the content of described streptococcus acidi lactici is 1 × 1014Individual/g, lactobacillus content is 1 × 1014Individual/g, Bacillus licheniformis content is 1 × 109Individual/g, all the other compositions are maize cob meal。
3. the feed additive of prevention tilapia enteritis according to claim 1 and 2, it is characterised in that described streptococcus acidi lactici, lactobacillus and Bacillus licheniformis are all isolatable from tilapia intestinal contents flora。
4. the feed additive of prevention tilapia enteritis according to claim 1 and 2 application in tilapia feed。
5. application according to claim 4, it is characterised in that described feed additive adding proportion in tilapia feed is 1-3%。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610121052.3A CN105685529A (en) | 2016-03-03 | 2016-03-03 | Feed additive for preventing tilapia enteritis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610121052.3A CN105685529A (en) | 2016-03-03 | 2016-03-03 | Feed additive for preventing tilapia enteritis |
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| CN105685529A true CN105685529A (en) | 2016-06-22 |
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| CN201610121052.3A Pending CN105685529A (en) | 2016-03-03 | 2016-03-03 | Feed additive for preventing tilapia enteritis |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109198266A (en) * | 2018-09-27 | 2019-01-15 | 清远海贝生物技术有限公司 | A kind of compound premix and its preparation method and application increasing strong fish enteron aisle |
| CN109619324A (en) * | 2018-12-05 | 2019-04-16 | 广西壮族自治区水产科学研究院 | A kind of aquatic feeds predigestion method and products thereof and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472862A (en) * | 2014-12-29 | 2015-04-01 | 江苏千药堂国医研究院有限公司 | Biological feed additive and preparation method thereof |
-
2016
- 2016-03-03 CN CN201610121052.3A patent/CN105685529A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472862A (en) * | 2014-12-29 | 2015-04-01 | 江苏千药堂国医研究院有限公司 | Biological feed additive and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 曾虹等: "《鱼虾饲料配方技术问答》", 31 August 2000, 中国农业科技出版社 * |
| 肖世玖: "三种添加剂对团头鲂和异育银鲫生长与消化机能的影响研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 赖凯昭: "饲料中添加复合益生菌对罗非鱼生长性能、肠道蛋白酶活性和饲料消化率的影响", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109198266A (en) * | 2018-09-27 | 2019-01-15 | 清远海贝生物技术有限公司 | A kind of compound premix and its preparation method and application increasing strong fish enteron aisle |
| CN109619324A (en) * | 2018-12-05 | 2019-04-16 | 广西壮族自治区水产科学研究院 | A kind of aquatic feeds predigestion method and products thereof and application |
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Application publication date: 20160622 |