CN107083347A - Biological agent and preparation method and application - Google Patents
Biological agent and preparation method and application Download PDFInfo
- Publication number
- CN107083347A CN107083347A CN201710438922.4A CN201710438922A CN107083347A CN 107083347 A CN107083347 A CN 107083347A CN 201710438922 A CN201710438922 A CN 201710438922A CN 107083347 A CN107083347 A CN 107083347A
- Authority
- CN
- China
- Prior art keywords
- biological agent
- raw material
- weight
- material components
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Abstract
The present invention relates to technical field of aquaculture, and in particular to a kind of biological agent and preparation method and application.Biological agent includes following raw material components：Lactic acid bacteria, bacillus and clostridium butyricum.Each raw material components of biological agent are inoculated in culture medium and carry out fermented and cultured, biological agent is obtained；In terms of being applied to aquaculture afterwards：Biological agent is directly used in water body, and per m3In water body, 5~10g biological agent is added.The biological agent that the present invention is provided, the water quality in aquaculture process can be not only effectively improved, significantly alleviate the problem of water pollution seriously causes water degradation, reduce the harmful substance in water quality, and feed can be used as, the appetite and upgrowth situation for raising aquatic products are effectively improved, is to kill two birds with one stone, has finally effectively facilitated sustainable development and the economic and social benefits of culture fishery.
Description
Technical field
The present invention relates to technical field of aquaculture, and in particular to a kind of biological agent and preparation method and application.
Background technology
China occupies critical role as the first in the world aquaculture big country, aquaculture in agricultural production；Especially
The southern china region of rivers and lakes, many areas all produce aquaculture as local farmers the important channel got rich, and aquaculture was already
One of pillar industry being increasingly becoming in cultivation agricultural.In recent years, with the fast development of intensive aquaculture industry, cultivation is dynamic
The reason such as thing excretion and bait surplus causes ammonia nitrogen in water body, nitrite equal size severe overweight, water quality deterioration, and then causes
Disease takes place frequently, and has had a strong impact on the sustainable health development of culture fishery.
The teaching of " breed fish and first support water " is followed, current people take a variety of for increasingly serious water pollution phenomenon
Measure, it is most commonly seen to be following several：First, physical method：Mechanical dredging is most common method, but mechanical dredging cost
Height, and there is scholar to think silt from the bottom of lake not pollution sources, desilting is only capable of improving water quality in a short time；Culturing pool is periodically changed
Water is also effective method, but cost is very high, and especially water resource is increasingly in short supply, it is difficult to implemented.2nd, chemical method：Often
There are copper sulphate, copper chelate, chlorine or oxychloride etc.；But, water and environment used for aquiculture are by serious in recent years
Pollution, the situation of aquatic products medication is increasingly severe, causes pathogenic bacteria to produce drug resistance, and aquaculture organism medicine residual is exceeded, to consumption
Person brings some diseases.3rd, bioelectric detecting and biological prosthetic：By the unique advantage of microorganism formulation, cultivation water is used it for
In body, the sustainable purpose of high-efficiency environment friendly is reached；However, at present microorganism formulation present on market exist mostly effect compared with
Difference, the higher shortcoming of cost.
Based on this, for more severe breeding water body pollution problem, there is provided a kind of effect is notable and lower-cost life
Thing preparation is particularly important.
The content of the invention
For defect of the prior art, the present invention is intended to provide a kind of biological agent and preparation method and application.This
The biological agent provided is provided, the water quality in aquaculture process can be not only effectively improved, significantly alleviates water pollution serious
Harmful substance in the problem of causing water degradation, reduction water quality, and the food for raising aquatic products can be effectively improved as feed
It is intended to and upgrowth situation, is to kill two birds with one stone, has finally effectively facilitated sustainable development and the economic society effect of culture fishery
Benefit.
Therefore, the present invention provides following technical scheme：
In a first aspect, the present invention provides a kind of biological agent, including following raw material components：Lactic acid bacteria, bacillus and
Clostridium butyricum；Wherein, the mass ratio of lactic acid bacteria, bacillus and clostridium butyricum is followed successively by (5~10)：(1~2)：(0.2~
0.6)。
In the further embodiment of the present invention, in addition to：Cellulase and spirulina；And lactic acid bacteria, cellulase
Mass ratio with spirulina is 1：(0.5~1.5)：(2~3).
The present invention provides a kind of preparation method of biological agent, comprises the following steps：By each raw material components of biological agent
It is inoculated in culture medium and carries out fermented and cultured, obtains biological agent；Wherein, the raw material components of culture medium by weight, including：
The parts by weight of wheat bran 50~100, the parts by weight of ammonium nitrate 5~10, the parts by weight of diammonium hydrogen phosphate 2~3 and the parts by weight of brown sugar 1~2.
In the further embodiment of the present invention, the raw material components of culture medium also include：The parts by weight of quassia 5~10, courage
The parts by weight of alkali 1~2 and the parts by weight of boric acid 2~5.
In the further embodiment of the present invention, after fermented and cultured, obtain also including at sterilizing before biological agent
Reason；Sterilization treatment is specifically included：By the product after fermented and cultured pressure be 150MPa~180MPa, temperature be 92 DEG C~95
DEG C, the time be 18min~25min under conditions of carry out the first autoclaving processing；It is afterwards 205MPa~230MPa in pressure,
Temperature be 98 DEG C~103 DEG C, the time be 5min~8min under conditions of carry out the second autoclaving processing.
In the further embodiment of the present invention, each raw material components of biological agent are inoculated in culture medium and sent out
Ferment culture is specifically included：Lactic acid bacteria and clostridium butyricum are inoculated in culture medium, are afterwards 3.8~4.2 in pH value, temperature is 28 DEG C
Ferment 50min~100min under conditions of~32 DEG C；Then it is inoculated with surplus stock in the product after fermentation 50min~100min
Component, afterwards in pH value 5.8~6.5, temperature is fermentation 10h~15h under conditions of 33 DEG C~35 DEG C.
In the further embodiment of the present invention, the preparation method of culture medium includes：Each raw material components are used into ball milling
Mode be well mixed, add water stir afterwards；Wherein, the addition quality of water is each raw material components gross mass of culture medium
1.5~3 times.
In the further embodiment of the present invention, the condition of ball milling is specifically：Rotating speed is 200rpm~350rpm, ball
Consume time as 3h~8h.
The third aspect, application of the biological agent that the present invention is provided in terms of aquaculture.
In the further embodiment of the present invention, the biological agent is directly used in water body, and per m3The water
In body, the 5~10g biological agent is added.
The above-mentioned technical proposal that the present invention is provided has advantages below：
(1) applicant has found by many experiments：The biological agent that the present invention is provided, can not only be effectively improved aquatic products and support
Water quality during growing, significantly alleviates the harmful substance in the problem of water pollution seriously causes water degradation, reduction water quality, and
And can be effectively improved the appetite and upgrowth situation for raising aquatic products as feed, be to kill two birds with one stone, finally effectively facilitate
The sustainable development of culture fishery and economic and social benefits.
(2) biological agent that provides of the present invention, can meet quality requirement of the market to feed product well, and by its
The appetite and health of aquatic products are raised in aquaculture process, can not only be effectively improved, breeding process reclaimed water is reduced
The cultivated animals death rate is produced, and any negative effect will not be produced to aquatic livestock.
(3) operating method that the present invention is provided is simple and convenient, easily operated and cost is relatively low, and aquatic products is most effectively facilitated at last
The sustainable development of aquaculture and economic and social benefits.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Embodiment
The embodiment to technical solution of the present invention is described in detail below.Following examples are only used for clearer
Explanation technical scheme, therefore be only used as example, and can not be limited the scope of the invention with this.
Experimental method in following embodiments, is conventional method unless otherwise specified.
Test material used, is to be commercially available from conventional reagent shop unless otherwise specified in following embodiments.
Quantitative test in following examples, is respectively provided with three repetition experiments, and data are the average value of three repetition experiments
Or mean+SD.
Lactic acid bacteria used in various embodiments of the present invention is purchased from Lai Sen Bioisystech Co., Ltd, and No. CAS is 2455423；Fourth
Sour clostridium is purchased from heavy chemicals sale Co., Ltd of Henan perseverance association；Strain bacillus is Virgibacillus
Carmonensis (culture presevation number：CGMCC 1.6151)；Cellulase is purchased from injury Bi Laiqing bio tech ltd,
Model COMBI.
The present invention provides a kind of biological agent, including following raw material components：Lactic acid bacteria, bacillus and clostridium butyricum；
Wherein, the mass ratio of lactic acid bacteria, bacillus and clostridium butyricum is followed successively by (5~10)：(1~2)：(0.2~0.6).
Preferably, in addition to：Cellulase and spirulina；And the mass ratio of lactic acid bacteria, cellulase and spirulina is 1：
(0.5~1.5)：(2~3).
In addition, the biological agent provided for the present invention, the present invention specially devises the preparation method of the biological agent, bag
Include following steps：
Each raw material components of biological agent are inoculated in culture medium and carry out fermented and cultured, biological agent is obtained；Wherein, train
Support base raw material components by weight, including：The parts by weight of wheat bran 50~100, the parts by weight of ammonium nitrate 5~10, phosphoric acid hydrogen two
The parts by weight of ammonium 2~3 and the parts by weight of brown sugar 1~2.Wherein, each raw material components of biological agent are inoculated in culture medium and sent out
Ferment culture is specifically included：Lactic acid bacteria and clostridium butyricum are inoculated in culture medium, are afterwards 3.8~4.2 in pH value, temperature is 28 DEG C
Ferment 50min~100min under conditions of~32 DEG C；Then it is inoculated with surplus stock in the product after fermentation 50min~100min
Component, afterwards in pH value 5.8~6.5, temperature is fermentation 10h~15h under conditions of 33 DEG C~35 DEG C.The preparation method of culture medium
Including：Each raw material components are well mixed by the way of ball milling, add water stirs afterwards；Wherein, the addition quality of water is
1.5~3 times of each raw material components gross mass of culture medium.The condition of ball milling is specifically：Rotating speed is 200rpm~350rpm, ball milling
Time is 3h~8h.
Preferably, the raw material components of culture medium also include：The parts by weight of quassia 5~10, the parts by weight of choline 1~2 and boric acid 2~
5 parts by weight.
Preferably, after fermented and cultured, obtain also including sterilization treatment before biological agent；Sterilization treatment is specifically included：
By the product after fermented and cultured pressure be 150MPa~180MPa, temperature be 92 DEG C~95 DEG C, the time be 18min~25min
Under conditions of carry out the first autoclaving processing；It is afterwards 205MPa~230MPa in pressure, temperature is 98 DEG C~103 DEG C, when
Between be 5min~8min under conditions of carry out the second autoclaving processing.
In addition, the biological agent that the present invention is provided can be effective in terms of aquaculture, concrete application method includes：Will
Biological agent is directly used in water body, and per m3In water body, 5~10g biological agent is added.
Illustrated with reference to embodiment：
Embodiment one
The present invention provides a kind of biological agent, including following raw material components：Lactic acid bacteria, bacillus and clostridium butyricum；
Wherein, the mass ratio of lactic acid bacteria, bacillus and clostridium butyricum is followed successively by 5：1：0.6.
The method provided using the present invention prepares biological agent, comprises the following steps：
Lactic acid bacteria and clostridium butyricum are inoculated in culture medium, are afterwards 3.8 in pH value, temperature is fermentation under conditions of 32 DEG C
50min；Then surplus stock component bacillus is inoculated with the product after fermentation 50min, afterwards in pH value 5.8, temperature is
Ferment 10h under conditions of 35 DEG C.In pressure it is 180MPa by the product after fermented and cultured 10h, temperature is 92 DEG C, and the time is
The first autoclaving processing is carried out under conditions of 18min；It is afterwards 205MPa in pressure, temperature is 103 DEG C, and the time is 5min's
Under the conditions of carry out the second autoclaving processing, finally give biological agent.
Wherein, the raw material components of culture medium by weight, including：The parts by weight of wheat bran 100, the parts by weight of ammonium nitrate 10,
The parts by weight of diammonium hydrogen phosphate 2 and the parts by weight of brown sugar 2；The preparation method of culture medium includes：It is in rotating speed by each raw material components
Ball milling 8h under conditions of 200rpm, adds water stir afterwards；The addition quality of water is the 3 of each raw material components gross mass of culture medium
Times.
Embodiment two
The present invention provides a kind of biological agent, including following raw material components：Lactic acid bacteria, bacillus and clostridium butyricum；
Wherein, the mass ratio of lactic acid bacteria, bacillus and clostridium butyricum is followed successively by 10：1：0.2.
The method provided using the present invention prepares biological agent, comprises the following steps：
Lactic acid bacteria and clostridium butyricum are inoculated in culture medium, are afterwards 4.2 in pH value, temperature is fermentation under conditions of 28 DEG C
100min；Then surplus stock component bacillus is inoculated with the product after fermentation 100min, afterwards in pH value 6.5, temperature
For the 15h that fermented under conditions of 33 DEG C.In pressure it is 150MPa by the product after fermented and cultured 15h, temperature is 95 DEG C, and the time is
The first autoclaving processing is carried out under conditions of 25min；It is afterwards 230MPa in pressure, temperature is 98 DEG C, and the time is 8min's
Under the conditions of carry out the second autoclaving processing, finally give biological agent.
Wherein, the raw material components of culture medium by weight, including：The parts by weight of wheat bran 50, the parts by weight of ammonium nitrate 5, phosphorus
The sour parts by weight of two ammonium of hydrogen 3 and the parts by weight of brown sugar 1；The preparation method of culture medium includes：By each raw material components rotating speed be 350rpm
Under conditions of ball milling 3h, add water stir afterwards；The addition quality of water is 1.5 times of each raw material components gross mass of culture medium.
Embodiment three
The present invention provides a kind of biological agent, including following raw material components：Lactic acid bacteria, bacillus and clostridium butyricum；
Wherein, the mass ratio of lactic acid bacteria, bacillus and clostridium butyricum is followed successively by 8：1：0.5.
The method provided using the present invention prepares biological agent, comprises the following steps：
Lactic acid bacteria and clostridium butyricum are inoculated in culture medium, are afterwards 4.0 in pH value, temperature is fermentation under conditions of 30 DEG C
80min；Then surplus stock component bacillus is inoculated with the product after fermentation 80min, afterwards in pH value 6.2, temperature is
Ferment 13h under conditions of 34 DEG C.In pressure it is 160MPa by the product after fermented and cultured 13h, temperature is 93 DEG C, and the time is
The first autoclaving processing is carried out under conditions of 20min；It is afterwards 215MPa in pressure, temperature is 100 DEG C, and the time is 6min's
Under the conditions of carry out the second autoclaving processing, finally give biological agent.
Wherein, the raw material components of culture medium by weight, including：The parts by weight of wheat bran 80, the parts by weight of ammonium nitrate 8, phosphorus
The sour parts by weight of two ammonium of hydrogen 2 and the parts by weight of brown sugar 1；The preparation method of culture medium includes：By each raw material components rotating speed be 300rpm
Under conditions of ball milling 5h, add water stir afterwards；The addition quality of water is 2 times of each raw material components gross mass of culture medium.
Example IV
The present invention provides a kind of biological agent, including following raw material components：Lactic acid bacteria, bacillus, clostridium butyricum, fiber
Plain enzyme and spirulina；Wherein, the mass ratio of lactic acid bacteria, bacillus, clostridium butyricum, cellulase and spirulina is followed successively by 10：
1：0.2：5：20.
The method provided using the present invention prepares biological agent, comprises the following steps：
Lactic acid bacteria and clostridium butyricum are inoculated in culture medium, are afterwards 4.2 in pH value, temperature is fermentation under conditions of 28 DEG C
100min；Then surplus stock component is inoculated with the product after fermentation 100min, afterwards in pH value 6.5, temperature is 33 DEG C
Under the conditions of ferment 15h.By the product after fermented and cultured 15h pressure be 150MPa, temperature be 95 DEG C, the time be 25min bar
The first autoclaving processing is carried out under part；It is afterwards 230MPa in pressure, temperature is 98 DEG C, and the time is progress under conditions of 8min
The processing of second autoclaving, finally gives biological agent.
Wherein, the raw material components of culture medium by weight, including：The parts by weight of wheat bran 50, the parts by weight of ammonium nitrate 5, phosphorus
The sour parts by weight of two ammonium of hydrogen 3 and the parts by weight of brown sugar 1；The preparation method of culture medium includes：By each raw material components rotating speed be 350rpm
Under conditions of ball milling 3h, add water stir afterwards；The addition quality of water is 1.5 times of each raw material components gross mass of culture medium.
Embodiment five
The present invention provides a kind of biological agent, including following raw material components：Lactic acid bacteria, bacillus, clostridium butyricum, fiber
Plain enzyme and spirulina；Wherein, the mass ratio of lactic acid bacteria, bacillus, clostridium butyricum, cellulase and spirulina is followed successively by 8：1：
0.5：10：15.
The method provided using the present invention prepares biological agent, comprises the following steps：
Lactic acid bacteria and clostridium butyricum are inoculated in culture medium, are afterwards 4.0 in pH value, temperature is fermentation under conditions of 30 DEG C
80min；Then surplus stock component is inoculated with the product after fermentation 80min, afterwards in pH value 6.2, temperature is 34 DEG C of bar
Ferment 13h under part.By the product after fermented and cultured 13h pressure be 160MPa, temperature be 93 DEG C, the time be 20min condition
The first autoclaving processing of lower progress；It is afterwards 215MPa in pressure, temperature is 100 DEG C, and the time is progress under conditions of 6min
The processing of second autoclaving, finally gives biological agent.
Wherein, the raw material components of culture medium by weight, including：The parts by weight of wheat bran 80, the parts by weight of ammonium nitrate 8, phosphorus
The sour parts by weight of two ammonium of hydrogen 2 and the parts by weight of brown sugar 1；The preparation method of culture medium includes：By each raw material components rotating speed be 300rpm
Under conditions of ball milling 5h, add water stir afterwards；The addition quality of water is 2 times of each raw material components gross mass of culture medium.
Embodiment six
The present invention provides a kind of biological agent, including following raw material components：Lactic acid bacteria, bacillus, clostridium butyricum, fiber
Plain enzyme and spirulina；Wherein, the mass ratio of lactic acid bacteria, bacillus, clostridium butyricum, cellulase and spirulina is followed successively by 8：1：
0.5：10：15.
The method provided using the present invention prepares biological agent, comprises the following steps：
Lactic acid bacteria and clostridium butyricum are inoculated in culture medium, are afterwards 4.0 in pH value, temperature is fermentation under conditions of 30 DEG C
80min；Then surplus stock component is inoculated with the product after fermentation 80min, afterwards in pH value 6.2, temperature is 34 DEG C of bar
Ferment 13h under part.By the product after fermented and cultured 13h pressure be 160MPa, temperature be 93 DEG C, the time be 20min condition
The first autoclaving processing of lower progress；It is afterwards 215MPa in pressure, temperature is 100 DEG C, and the time is progress under conditions of 6min
The processing of second autoclaving, finally gives biological agent.
Wherein, the raw material components of culture medium by weight, including：The parts by weight of wheat bran 80, the parts by weight of ammonium nitrate 8, phosphorus
The sour parts by weight of two ammonium of hydrogen 2, the parts by weight of brown sugar 1, the parts by weight of quassia 10, the parts by weight of choline 1 and the parts by weight of boric acid 5；The system of culture medium
Preparation Method includes：By each raw material components rotating speed be 300rpm under conditions of ball milling 5h, add water stir afterwards；The addition of water
Quality is 2 times of each raw material components gross mass of culture medium.
In addition, the advantage in order to further highlight the preparation method that the present invention is provided, carries out following contrast experiment；It is right below
The unitary variant experiment of relevant parameter is carried out on this basis as benchmark using embodiment six than experiment.
Inoculation method used is different from embodiment six in the comparative example, the equal be the same as Example six of remaining parameter.
Specifically, this comparative example provides a kind of biological agent, including following raw material components：Lactic acid bacteria, bacillus, butyric acid
Clostridium, cellulase and spirulina；Wherein, the mass ratio of lactic acid bacteria, bacillus, clostridium butyricum, cellulase and spirulina
It is followed successively by 8：1：0.5：10：15.
Biological agent is prepared, is comprised the following steps：
Each raw material components of biological agent are inoculated in culture medium, are afterwards 4.0 in pH value, temperature is 30 DEG C of condition
Lower fermentation 13h.By the product after fermented and cultured 13h pressure be 160MPa, temperature be 93 DEG C, the time be 20min under conditions of
Carry out the first autoclaving processing；It is afterwards 215MPa in pressure, temperature is 100 DEG C, the time is to carry out the under conditions of 6min
The processing of two autoclavings, finally gives biological agent.Wherein, the raw material components of culture medium and preparation method be the same as Example six.
In addition, the biological agent that various embodiments of the present invention and comparative example are obtained, it is evaluated by function assessment pilot system
Effect.
First, each embodiment biological agent safety indexes detection
Specifically, in the biological agent obtained to each embodiment according to forage health standard GB13078-2001, inventor
Content of microorganisms carried out analysis detection.And detected for content of microorganisms, detection respectively places that (normal temperature avoid light place is
Can) 1 month and 1 year when content, detection data it is as shown in table 1.
Harmful substance contents in each embodiment of table 1 and comparative example biological agent
2nd, performance detection of each embodiment biological agent to aquatic products health status
The biological agent that various embodiments of the present invention and comparative example are obtained, according to every m3Water body 8g addition is used to cultivate
In water body, performance test is carried out after two months.Choose in 8 5m × 5m experiment sample prescription, each experiment sample prescription and raise 100 crucian carps
Fish fingerling.First to the 6th experiment sample prescription tries out the embodiment of the present invention one into embodiment six respectively as experimental group
Biological agent；7th and the 8th as a control group, biological agent and commercially available fish meal in comparative example is tried out respectively, daily
Twice (07:30 and 19:30).According to forage health standard aquatic feeds trophic level Q/QJ.SH-06-2009, each implement is determined
The health status of carp in example and comparative example, in addition, determining the fish digestible energy of biological agent in each embodiment and comparative example, can disappearing
Change protein content, crude fibre and available phosphorus content, concrete outcome is as shown in table 2.In addition, determine respectively it is each experiment sample prescription the 0th day,
1st month and water quality during second month, the specific pH value for determining water body, dissolved oxygen (DO), ammoniacal nitrogen (NH3- N), nitrous acid
Salt (NO2- N) and chemical oxygen consumption (COC) (COD), specific data are as shown in Table 3 and Table 4.
Performance test of the biological agent of each embodiment of table 2 and control group in feeding fish
Improvement situation list one of the biological agent of each embodiment of table 3 and control group to water quality
Improvement situation list two of the biological agent of each embodiment of table 4 and control group to water quality
Certainly, except the situation that embodiment one is enumerated to embodiment six, the species and proportioning of other raw material components, prepared
Actual conditions in journey etc. is also possible.
The biological agent that the present invention is provided, can not only be effectively improved the water quality in aquaculture process, significantly alleviate water
Harmful substance in the problem of body pollution seriously causes water degradation, reduction water quality, and feeding can be effectively improved as feed
Support aquatic products appetite and upgrowth situation, be to kill two birds with one stone, finally effectively facilitated culture fishery sustainable development and
Economic and social benefits.
In the description of the invention, it is to be understood that term " first ", " second " are only used for describing purpose, and can not
It is interpreted as indicating or implies relative importance or the implicit quantity for indicating indicated technical characteristic.Thus, define " the
One ", one or more this feature can be expressed or be implicitly included to the feature of " second ".In the description of the invention,
" multiple " are meant that two or more, unless otherwise specifically defined.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means to combine specific features, structure, material or the spy that the embodiment or example are described
Point is contained at least one embodiment of the present invention or example.In this manual, to the schematic representation of above-mentioned term not
Identical embodiment or example must be directed to.Moreover, specific features, structure, material or the feature of description can be with office
Combined in an appropriate manner in one or more embodiments or example.In addition, in the case of not conflicting, the skill of this area
Art personnel can be tied the not be the same as Example or the feature of example and non-be the same as Example or example described in this specification
Close and combine.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changed, replacing and modification.
Claims (10)
1. a kind of biological agent, it is characterised in that including following raw material components：Lactic acid bacteria, bacillus and clostridium butyricum；
Wherein, the mass ratio of the lactic acid bacteria, the bacillus and the clostridium butyricum is followed successively by (5~10)：(1~2)：
(0.2~0.6).
2. biological agent according to claim 1, it is characterised in that also include：
Cellulase and spirulina；And the mass ratio of the lactic acid bacteria, the cellulase and the spirulina is 1：(0.5~
1.5)：(2~3).
3. the preparation method of biological agent described in claim 1 or 2, it is characterised in that comprise the following steps：
Each raw material components of the biological agent are inoculated in culture medium and carry out fermented and cultured, the biological agent is obtained；
Wherein, the raw material components of the culture medium by weight, including：The parts by weight of wheat bran 50~100, ammonium nitrate 5~10
Parts by weight, the parts by weight of diammonium hydrogen phosphate 2~3 and the parts by weight of brown sugar 1~2.
4. the preparation method of biological agent according to claim 3, it is characterised in that：
The raw material components of the culture medium also include：The parts by weight of quassia 5~10, the parts by weight of choline 1~2 and the weight of boric acid 2~5
Part.
5. the preparation method of biological agent according to claim 3, it is characterised in that：
It is described to obtain also including sterilization treatment before the biological agent after the fermented and cultured；
The sterilization treatment is specifically included：In pressure it is 150MPa~180MPa by the product after the fermented and cultured, temperature is
92 DEG C~95 DEG C, the time be 18min~25min under conditions of carry out the first autoclaving processing；It is afterwards 205MPa in pressure
~230MPa, temperature be 98 DEG C~103 DEG C, the time be 5min~8min under conditions of carry out the second autoclaving processing.
6. the preparation method of biological agent according to claim 3, it is characterised in that：
It is described by each raw material components of biological agent be inoculated in culture medium carry out fermented and cultured specifically include：
Lactic acid bacteria and clostridium butyricum are inoculated in culture medium, are afterwards 3.8~4.2 in pH value, temperature is 28 DEG C~32 DEG C of bar
Ferment 50min~100min under part；Then surplus stock component is inoculated with the product after the fermentation 50min~100min,
Afterwards in pH value 5.8~6.5, temperature is fermentation 10h~15h under conditions of 33 DEG C~35 DEG C.
7. the preparation method of biological agent according to claim 3, it is characterised in that：
The preparation method of the culture medium includes：Each raw material components are well mixed by the way of ball milling, add water stirring afterwards
Uniformly；Wherein, the addition quality of the water is 1.5~3 times of each raw material components gross mass of the culture medium.
8. the preparation method of biological agent according to claim 7, it is characterised in that：
The condition of the ball milling is specifically：Rotating speed is 200rpm~350rpm, and Ball-milling Time is 3h~8h.
9. application of any one of claim 1 or 2 biological agent in terms of aquaculture.
10. application according to claim 9, it is characterised in that：
The biological agent is directly used in water body, and per m3In the water body, the 5~10g biological agent is added.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710438922.4A CN107083347A (en) | 2017-06-12 | 2017-06-12 | Biological agent and preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710438922.4A CN107083347A (en) | 2017-06-12 | 2017-06-12 | Biological agent and preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107083347A true CN107083347A (en) | 2017-08-22 |
Family
ID=59606139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710438922.4A Pending CN107083347A (en) | 2017-06-12 | 2017-06-12 | Biological agent and preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107083347A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111732208A (en) * | 2020-04-24 | 2020-10-02 | 嘉兴市禾晟生物制品有限公司 | Composite water purifying agent and preparation method thereof |
| CN113604380A (en) * | 2021-07-15 | 2021-11-05 | 山东益昊生物科技有限公司 | Method for producing composite microbial inoculum by fermenting clostridium butyricum centrifugal waste liquid, corresponding composite microbial inoculum and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690545A (en) * | 2009-09-07 | 2010-04-07 | 肇东市日成酶制剂有限公司 | Method for producing complex micro-ecological preparation with microbial agents and enzyme |
| CN104176834A (en) * | 2014-08-21 | 2014-12-03 | 湖南农业大学 | Freshwater compound microorganism substrate modifier |
| CN104431621A (en) * | 2014-12-23 | 2015-03-25 | 广东海大集团股份有限公司 | Compound premix for aquatic products and preparation method of compound premix |
| CN105815609A (en) * | 2016-04-05 | 2016-08-03 | 山东省海洋生物研究院 | Complete formula feed for young apostichopus japonicas and preparation method of complete formula feed |
-
2017
- 2017-06-12 CN CN201710438922.4A patent/CN107083347A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690545A (en) * | 2009-09-07 | 2010-04-07 | 肇东市日成酶制剂有限公司 | Method for producing complex micro-ecological preparation with microbial agents and enzyme |
| CN104176834A (en) * | 2014-08-21 | 2014-12-03 | 湖南农业大学 | Freshwater compound microorganism substrate modifier |
| CN104431621A (en) * | 2014-12-23 | 2015-03-25 | 广东海大集团股份有限公司 | Compound premix for aquatic products and preparation method of compound premix |
| CN105815609A (en) * | 2016-04-05 | 2016-08-03 | 山东省海洋生物研究院 | Complete formula feed for young apostichopus japonicas and preparation method of complete formula feed |
Non-Patent Citations (7)
| Title |
|---|
| 桂国弘 等: ""丁酸梭菌调节肠道健康的作用机制"", 《饲料研究》 * |
| 潘春梅: "《微生态制剂生产及应用》", 30 September 2014, 中国农业大学出版社 * |
| 熊继东: ""浅析微生态制剂在水产养殖中的应用"", 《现代化农业》 * |
| 王如才 等: "《海水养殖技术手册》", 30 November 2001, 上海科学技术出版社 * |
| 袁昌齐 等: "《世界植物药》", 31 January 2013, 东南大学出版社 * |
| 赵刚 等: "《小水体养殖》", 30 April 2014, 海洋出版社 * |
| 顾德平 等: "《水产动物用药技术问答》", 30 September 2010, 金盾出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111732208A (en) * | 2020-04-24 | 2020-10-02 | 嘉兴市禾晟生物制品有限公司 | Composite water purifying agent and preparation method thereof |
| CN113604380A (en) * | 2021-07-15 | 2021-11-05 | 山东益昊生物科技有限公司 | Method for producing composite microbial inoculum by fermenting clostridium butyricum centrifugal waste liquid, corresponding composite microbial inoculum and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104082345B (en) | A kind of aquaculture special EM bacterium powder and its preparation method and application | |
| CN107324504B (en) | A kind of compound algae microbial inoculum and preparation method thereof for culture-pool water quality improvement | |
| CN105462878B (en) | A kind of efficient decomposing agent of feces of livestock and poultry and its fermentation process | |
| CN101099543B (en) | Aquatic biological feed and preparation method thereof | |
| CN106380004B (en) | Aquaculture waters restoration of the ecosystem agent and preparation method thereof | |
| CN102007876A (en) | Biological enzyme fish guano and preparation method thereof | |
| CN102429127A (en) | Aquatic bio-feed | |
| CN110342991A (en) | From the organic fertilizer of fermentation cordyceps sinensis | |
| CN106754527A (en) | A kind of Chinese herbal medicine compound micro-ecological preparation and its production and use | |
| CN107653200A (en) | A kind of microbial bacterial agent for promoting dead pig corpse aerobic compost and application | |
| CN107828698A (en) | A kind of composite bacteria preparation and its preparation method and application | |
| CN105543131A (en) | Compound bacteria/cottonseed meal fermented feed and preparation method thereof | |
| CN104585510B (en) | A kind of mixed fungus fermentation thing feed addictive and preparation method thereof | |
| CN108865935A (en) | A kind of probiotics of water quality improvement and preparation method thereof | |
| CN107083347A (en) | Biological agent and preparation method and application | |
| CN108017465A (en) | Purification soil and salt-soda soil microbial organic fertilizer and preparation method thereof of pH value can be reduced | |
| CN105272556A (en) | Aquaculture biofertilizer and preparation method thereof | |
| CN104726378A (en) | Method for improving protective enzyme activities of salt-stressed turfgrass by adopting enhanced salt-tolerant microbial agent | |
| CN102140041B (en) | Microbial fertilizer for shrimp pond and preparation method thereof | |
| CN103194393A (en) | Fermentation bed fungicide and preparation method thereof | |
| CN102940145A (en) | Preparation method of fermentation aquatic health care feed | |
| CN101082034A (en) | Preparation of phycomycetes bacterium and method for restoring aquaculture environment | |
| CN102302092A (en) | Fermented feed for young animals and preparation method thereof | |
| CN104072298B (en) | Biological fish manure and preparation method thereof | |
| CN103145460A (en) | Bio-organic fertilizer for preventing disease for aquaculture and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170822 |