CN102940145A - Preparation method of fermentation aquatic health care feed - Google Patents
Preparation method of fermentation aquatic health care feed Download PDFInfo
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- CN102940145A CN102940145A CN2012104978917A CN201210497891A CN102940145A CN 102940145 A CN102940145 A CN 102940145A CN 2012104978917 A CN2012104978917 A CN 2012104978917A CN 201210497891 A CN201210497891 A CN 201210497891A CN 102940145 A CN102940145 A CN 102940145A
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Abstract
The invention discloses a preparation method of fermentation aquatic health care feed. The preparation method aims at providing safe, reliable environment-friendly fermentation feed for aquatic animals. The preparation method includes the following steps: 1) preparing first-level strain; (2) preparing second-level strain, introducing saccharomycete liquid and lactic acid bacteria liquid prepared in the step 1) according to the inoculum size with the volume ratio being 0.5-5% into a seed fermentation tank filled with a liquid saccharomycete seed medium and standing for culture; 3) preparing third-level strain and respectively introducing the saccharomycete liquid and the saccharomycete liquid of the lactic acid bacteria liquid prepared in the step 2) into the fermentation tank filled with a saccharomycete minimal medium and a lactic acid bacteria minimal medium for culture according to the inoculum size with the volume ratio being 2-10%; step 4) inoculating, inoculating lactic acid bacteria three-level culture and fermentation three-level culture obtained in the step 3) to basic feed according to the ratio of 0.25-2% by mass, evenly mixing the two cultures and adjusting moisture to enable the final density to be 34-38%. The preparation method belongs to the technical field of feeds.
Description
Technical field
The present invention relates to the preparation of biological health feed, specifically, is a kind of preparation method of fermented type aquatic products health-care feed, belongs to field of feed.
Background technology
Along with the fast development of animal husbandry, a large amount of uses of the medicines such as antibiotic, feed safety (food security) problem has become aquaculture and the common focus of paying close attention to of consumer.World Developed Countries government has assigned in succession to ordering within a certain time and ban that antibiotic uses.European countries completely forbid over past ten years and add antibiotic in feeds, and the Antibiotics that China ratifies to use in feed is also reducing gradually.In the face of situation like this, we must do from the source, and fermented feed is a good approach.
Fermented feed refers under the manual control condition, beneficial microbe is by the metabolic activity of self, vegetalitas, animality and mineral raw material are decomposed or is converted into the bio-safety feed that microbial bacteria body protein, biologically active peptide, amino acid, immunopotentiator, probio, organic acid, vitamin, complex enzyme formulation and UGF (GDF) are integrated.This is the process of an external biological effect, is equivalent to the increase of Alimentary external prolongation and digestion time.Feed smell acid after external predigestion is fragrant, is easy to digestion, absorbs, thereby improved the palatability of feed and digested and absorbed rate, has accelerated the healthy growth of animal, reduces feed cost.In addition, by sweat, also can eliminate ANFs, poisonous and harmful substances in the raw material, can also greatly reduce the use of antibiotic etc class additive, thereby reach the purpose that safety is fed.
Really, be not all fermented feeds all be " safety fermented feed ".At present, the production of China's fermented feed faces following problem: Zymolysis Equipment falls behind, the professional lacks; Management, sanitary condition is poor; Dangerous raw material fermentation.So-called " raw material " refer to the raw material that do not carry out disinfection, and this will cause the toxin in the raw material thoroughly not removed.In fermented feed, add the mold toxin sorbents such as immune star (main component is: manna oligosacchride 〉=20%, beta glucan 〉=20%), improve the report of fermented feed security, so far there are no relevant report.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of preparation method of fermented type aquatic products health-care feed is provided, the aquatic products complete feed is fermented, safe and reliable, environmental protection aquatic livestock fermented feed.
The present invention is achieved by the following technical solutions, the present invention includes following steps:
(1) first class inoculum preparation
Picking one ring saccharomycete is inoculated in the sub-culture medium of yeast solid bacterial classification from the candida utili bacterium test tube solid slant medium, under 28 ~ 35 ℃ of incubators, cultivates after 24～48 hours, makes the saccharomycete first class inoculum; Picking one ring lactic acid bacteria from the lactic acid bacteria test tube solid slant medium is inoculated into and fills in the lactic acid bacteria liquid seed culture medium triangular flask, under 35 ~ 40 ℃, leaves standstill and cultivates 24～48 hours, makes the lactic acid bacteria first class inoculum;
(2) second class inoculum preparation
The inoculum concentration access by 0.5～5% volume ratio of the saccharomycete bacterium liquid that makes in the step (1) is filled in the seed fermentation tank of the sub-culture medium of liquid yeast bacterial classification, air agitation fermentation 24～72 hours, temperature is 28～37 ℃, makes the saccharomycete second class inoculum; The lactic acid bacterial liquid that step (1) is made fills in the seed fermentation tank of the sub-culture medium of liquid lactobacillus inoculation by the inoculum concentration access of 0.5～5% volume ratio, leaves standstill and cultivates 24～48 hours, and temperature is 35～40 ℃, makes the lactic acid bacteria second class inoculum;
(3) three-class strain preparation
Inoculum concentration by 2～10% volume ratios fills the saccharomycete bacterium liquid access that makes in the step (2) in the fermentation tank of saccharomycete minimal medium, and adjusting the pH value is 6.5～7.5, and air agitation fermentation 24～72 hours makes the saccharomycete three-class strain; Inoculum concentration by 2～10% volume ratios fills the lactic acid bacterial liquid access that makes in the step (2) in the fermentation tank of lactic acid bacteria minimal medium, and adjusting the pH value is 5.5～6, stirs fermentation 24～72 hours, makes the lactic acid bacteria three-class strain;
(4) inoculation
With dregs of beans 60 ~ 75%, corn flour 10 ~ 20%, wheat bran 5 ~ 10%, take off nuclear yeast 3 ~ 10%, immunity star 1 ~ 8%, vegetable oil 1 ~ 3%, salt 0.1 ~ 0.5%, calcium dihydrogen phosphate 0.5 ~ 1%, the many ore deposits of aquatic products 0.05 ~ 0.2%, 50% Choline Chloride 0.1 ~ 0.3% is mixed with basal feed, and the lactic acid bacteria three-class strain of step (3) gained and saccharomycete three-class strain are seeded to basal feed with 0.25 ~ 2% mass fraction respectively, and mix, regulating moisture to final concentration is 34 ~ 38%;
(5) packing
With the feed in (4) pack into settle the polybag of check valve after, enclosed package, under the room temperature, spontaneous fermentation 10 ~ 25 days gets product.
The preparation method of above-mentioned fermented type aquatic products health-care feed, candida utili bacterium slant medium comprises following component described in the step (1): glucose 5g/L, peptone 2g/L, yeast extract 1g/L, sodium chloride 0.5g/L, agar 15g/L, the pH of saccharomycete slant medium are 6.5～7.5, sterilising conditions: 115 ℃ of lower sterilization 20min; Described lactic acid bacteria slant medium comprises following component: skimmed milk power 100.0g/L, dusty yeast 1g/L, K
2HPO
45g/L, agar 15g/L, sterilising conditions: 115 ℃ of lower sterilization 20min.
The preparation method of above-mentioned fermented type aquatic products health-care feed, saccharomycete seed culture medium comprises following component described in the step (2): glucose 10 g/L, urea 1g/L, K
2HPO
40.1g/L, MgSO
47H
2O 0.1g/L, corn steep liquor 0.6g, the pH of saccharomycete seed culture medium is 6.5～7.5; Described lactic acid bacteria seed culture medium comprises following component: glucose 20.0g/L, peptone 10.0g/L, beef extract 10.0g/L, yeast extract 5.0g/L, anhydrous sodium acetate 5.0g/L, K
2HPO
42.0g/L, diammonium hydrogen citrate 2.0g/L, MgSO
47H
2O 0.58g/L, MnSO
4H
2O 0.33g/L, the pH of lactic acid bacteria seed culture medium is 6.0～6.5.
The preparation method of above-mentioned fermented type aquatic products health-care feed, the saccharomycete minimal medium comprises following component described in the step (3): glucose 10g/L, urea 1g/L, K
2HPO
40.1g/L, MgSO
47H
2O 0.2g/L, corn steep liquor 0.1g/L, CaCO
33g/L, pH are 6.5～7.5; Described lactic acid bacteria minimal medium comprises following component: glucose 20.0g/L, peptone 10.0g/L, beef extract 10.0g/L, yeast extract 5.0g/L, anhydrous sodium acetate 5.0g/L, dipotassium hydrogen phosphate 2.0g/L, diammonium hydrogen citrate 2.0g/L, MgSO47H
2O 0.58g/L, MnSO4H
2O 0.33g/L, pH are 6.0～6.5.
The preparation method of above-mentioned fermented type aquatic products health-care feed, the pH value in the step (3) is 5.5～6 with pH in the NaOH solution adjustment tank of mass concentration 20～30%.
Compared with prior art, the present invention has following beneficial effect:
1, the present invention has abandoned the low requirement of traditional zymotic to raw material, the special detection that proposes harmful microorganism and mycotoxin etc., the security that improves feed from the source;
2, combine the use of microbial fermentation and immune star, make the residual a small amount of poisonous and harmful substance of possibility in the raw material, can thoroughly be removed, further consolidated feed safety;
3, take off the use of nuclear yeast, improved immunity and the survival rate of aquatic livestock, increased output and cultivation income.
4, in the finished product, the beneficial microorganism amount reproduction has increased mycoprotein; The lactic acid that lactic acid bacteria produces plays certain regulating action to the enteron aisle of aquatic livestock; Through long-time, fully fermentation, microorganisms a large amount of enzymes, polysaccharide, aldehydes matter, the feed ANFs of both having eliminated, again the large molecule in the feed is converted into little molecule, like this, nutrition in the feed can fully be absorbed, utilize by aquatic livestock, and the nutrients loss in its excreta and the excreta, is conducive to reduce the pressure of water body environment and growing of pathogenic microorganism;
5, the finished product purposes of gained is wide, is mainly used in part and substitutes medium-to-high grade aquatic animal feed.
6, feed safety is reliable, environmental protection.
The specific embodiment
Below in conjunction with the specific embodiment, the present invention is described in further detail, but do not consist of any limitation of the invention.
Embodiment 1
(1) first class inoculum preparation
Picking one ring saccharomycete is inoculated in the sub-culture medium of yeast solid bacterial classification, under 30 ℃ of incubators from the saccharomycete test tube solid slant medium, speed of agitator 150rpm, cultivate after 24 hours, make the saccharomycete first class inoculum, activate and observe its purity and do not find miscellaneous bacteria; In like manner, picking one ring lactic acid bacteria from the lactic acid bacteria test tube solid slant medium is inoculated into and fills in the lactic acid bacteria liquid seed culture medium triangular flask, under 35 ℃, leaves standstill and cultivates 24 hours, makes the lactic acid bacteria first class inoculum, and activation and microscopy bacterial classification purity are not found miscellaneous bacteria;
(2) second class inoculum preparation
With the saccharomycete bacterium liquid that makes in the step (1) by 1%(v/v) the inoculum concentration access fill in the seed fermentation tank of the sub-culture medium of liquid yeast bacterial classification, air agitation fermentation 24 hours, 28 ℃ of temperature, speed of agitator 280rpm, throughput 2L/(Lmin), detect bacterial concentration and purity, OD600 numerical value is 0.65, generate without miscellaneous bacteria, make the saccharomycete second class inoculum; The lactic acid bacterial liquid that step (1) is made is by 1%(v/v) the inoculum concentration access fill in the seed fermentation tank of the sub-culture medium of liquid lactobacillus inoculation, leave standstill and cultivated 24 hours, detect bacterial concentration and purity, make the lactic acid bacteria second class inoculum, OD600 numerical value is 0.65, generates without miscellaneous bacteria;
(3) three-class strain preparation
By 2%(v/v) inoculum concentration the access of the saccharomycete bacterium liquid that makes in the step (2) is filled in the fermentation tank of saccharomycete minimal medium, adjusting the pH value is 6.5, air agitation fermentation 24 hours, temperature is 28 ℃, speed of agitator 500rpm, throughput 2L/(Lmin), detect bacterial concentration and purity, OD600 numerical value is 0.65, generates without miscellaneous bacteria, makes the saccharomycete three-class strain; By 2%(v/v) inoculum concentration the access of the lactic acid bacterial liquid that makes in the step (2) is filled in the fermentation tank of lactic acid bacteria minimal medium, adjusting the pH value with the NaOH solution of mass concentration 25% is 5.5, stir fermentation 24 hours, 37 ℃ of temperature, speed of agitator 70rpm detects bacterial concentration and purity, OD600 numerical value is 0.65, generates without miscellaneous bacteria, makes the lactic acid bacteria three-class strain;
(4) inoculation
With dregs of beans (protein content 43%) 65%, corn flour 15%, wheat bran 5% takes off nuclear yeast 7%, immune star 3%, vegetable oil 2%, salt 0.2%, calcium dihydrogen phosphate 0.6%, the many ore deposits of aquatic products 0.1%, 50% Choline Chloride 0.1% is mixed with basal feed.Wherein, each component in the basal feed detects respectively the indexs such as mould, bacterium, salmonella, aflatoxin by the requirement of forage health standard.Detect qualified after, pulverizer is pulverized and mixed the preparation basal feed.The lactic acid bacteria three-class strain of step (3) gained and saccharomycete three-class strain respectively with 1% mass fraction, are seeded in the basal feed, and mix, and regulating moisture to final concentration is 35%.
(5) packing
With the feed in (4) pack into settle the polybag of check valve (gas can be discharged in the bag, and bag outer gas can not enter, and can prevent entering of harmful bacteria in the air) after, enclosed package under 30 ℃, is fermented more than 15 days, gets product.
Substitute penaeus vannamei boone feed with this feed, before the 5cm, substitute traditional shrimp material of 10%, substitute 5% traditional shrimp material behind the 5cm, the result shows, compares with control group, and material is than reducing by 0.15, the incidence of disease reduces by 8.6%, reduces medication and changes water rate with 146 yuan/mu, improves output 7.8%.
Embodiment 2
(1) first class inoculum preparation
Picking one ring saccharomycete is inoculated in the sub-culture medium of yeast solid bacterial classification, under 30 ℃ of incubators from the saccharomycete test tube solid slant medium, speed of agitator 260rpm cultivated after 24 hours, made the saccharomycete first class inoculum, activation is also observed its purity, and OD600 numerical value is 0.60, generates without miscellaneous bacteria; In like manner, picking one ring lactic acid bacteria from the lactic acid bacteria test tube solid slant medium is inoculated into and fills in the lactic acid bacteria liquid seed culture medium triangular flask, under 37 ℃, leave standstill and cultivated 24 hours, activation and microscopy bacterial classification purity, OD600 numerical value is 0.65, makes the lactic acid bacteria first class inoculum, generates without miscellaneous bacteria;
(2) second class inoculum preparation
With the saccharomycete first class inoculum that makes in the step (1) by 2%(v/v) the inoculum concentration access fill in the seed fermentation tank of the sub-culture medium of liquid yeast bacterial classification, air agitation fermentation 48 hours, 37 ℃ of temperature, speed of agitator 500rpm, throughput 3L/(Lmin), detect bacterial concentration and purity, OD600 numerical value is 0.75, generate without miscellaneous bacteria, make the saccharomycete second class inoculum; The lactic acid bacteria first class inoculum that step (1) is made is by 2%(v/v) the inoculum concentration access fill in the seed fermentation tank of the sub-culture medium of liquid lactobacillus inoculation, leave standstill and cultivated 48 hours, make the lactic acid bacteria second class inoculum, detect bacterial concentration and purity, OD600 numerical value is 0.71, generates without miscellaneous bacteria;
(3) three-class strain preparation
By 2%(v/v) inoculum concentration the access of the saccharomycete second class inoculum that makes in the step (2) is filled in the fermentation tank of saccharomycete minimal medium, adjusting the pH value is 7.0, air agitation fermentation 48 hours, temperature is 37 ℃, speed of agitator 500rpm, throughput 4L/(Lmin) detects bacterial concentration and purity, make the saccharomycete three-class strain; By 3%(v/v) inoculum concentration the access of the lactic acid bacteria second class inoculum that makes in the step (2) is filled in the fermentation tank of lactic acid bacteria minimal medium, adjusting the pH value with the NaOH solution of mass concentration 20% is 5.8, stir fermentation 48 hours, 38 ℃ of temperature, speed of agitator 100rpm; Detect bacterial concentration and purity, OD600 numerical value is 0.65, generates without miscellaneous bacteria, makes the lactic acid bacteria three-class strain;
(4) inoculation
With dregs of beans (protein content 43%) 70%, corn flour 8%, wheat bran 5%, take off nuclear yeast 10%, immunity star 3%, vegetable oil 1%, salt 0.3, calcium dihydrogen phosphate 0.5%, the many ore deposits of aquatic products 0.1%, 50% Choline Chloride 0.1% is mixed with basal feed, wherein, each component in the basal feed detects respectively the indexs such as mould, bacterium, salmonella, aflatoxin by the requirement of forage health standard.Detect qualified after, pulverizer is pulverized and mixed the preparation basal feed.The lactic acid bacteria three-class strain of step (3) gained is seeded to basal feed with 0.25% mass fraction, and the saccharomycete three-class strain is seeded to basal feed with 2.5% mass fraction, and mixes, and regulating moisture to final concentration is 36%.
(5) packing
With the feed in (4) pack into settle the polybag of check valve (gas can be discharged in the bag, and bag outer gas can not enter, and can prevent entering of harmful bacteria in the air) after, enclosed package under 37 ℃, is fermented more than 12 days, can use.
Replace traditional eel material Japanese eel of feeding with this feed 5%, compare with control group, in the experimental group, the eel speed of growth is very fast, listing in 5 days in advance; M ﹠ M difference 6.3% and 4.1%, wherein, the enteritis incidence of disease reduces by 15.6%.
Embodiment 3
(1) first class inoculum preparation
Picking one ring saccharomycete is inoculated in the sub-culture medium of yeast solid bacterial classification, under 35 ℃ of incubators from the saccharomycete test tube solid slant medium, speed of agitator 200rpm cultivated after 24 hours, made the saccharomycete first class inoculum, activation is also observed its purity, and OD600 numerical value is 0.75, generates without miscellaneous bacteria; In like manner, picking one ring lactic acid bacteria from the lactic acid bacteria test tube solid slant medium is inoculated into and fills in the lactic acid bacteria liquid seed culture medium triangular flask, under 35 ℃, leave standstill and cultivated 24 hours, make the lactic acid bacteria first class inoculum, activation and microscopy bacterial classification purity, OD600 numerical value is 0.80, generates without miscellaneous bacteria;
(2) second class inoculum preparation
With the saccharomycete first class inoculum that makes in the step (1) by 3%(v/v) the inoculum concentration access fill in the seed fermentation tank of the sub-culture medium of liquid yeast bacterial classification, air agitation fermentation 43 hours, 30 ℃ of temperature, speed of agitator 350rpm, throughput 2.5L/(Lmin), detect bacterial concentration and purity, OD600 numerical value is 0.75, generate without miscellaneous bacteria, make the saccharomycete second class inoculum; The lactic acid bacteria first class inoculum that step (1) is made is by 3%(v/v) the inoculum concentration access fill in the seed fermentation tank of the sub-culture medium of liquid lactobacillus inoculation, leave standstill and cultivated 43 hours, make the lactic acid bacteria second class inoculum, detect bacterial concentration and purity, OD600 numerical value is 0.70, generates without miscellaneous bacteria;
(3) three-class strain preparation
By 5%(v/v) inoculum concentration the access of the saccharomycete second class inoculum that makes in the step (2) is filled in the fermentation tank of saccharomycete minimal medium, adjusting the pH value is 6.5, air agitation fermentation 24 hours, temperature is 30 ℃, speed of agitator 400rpm, throughput 4L/(Lmin) detect bacterial concentration and purity, OD600 numerical value is 0.80, generate without miscellaneous bacteria, make the saccharomycete three-class strain; By 4%(v/v) inoculum concentration the access of the lactic acid bacteria second class inoculum that makes in the step (2) is filled in the fermentation tank of lactic acid bacteria minimal medium, adjusting the pH value with the NaOH solution of mass concentration 30% is 5.5, stir fermentation 24 hours, 40 ℃ of temperature, speed of agitator 50rpm detects bacterial concentration and purity, and OD600 numerical value is 0.65, generate without miscellaneous bacteria, make the lactic acid bacteria three-class strain;
(4) inoculation
With dregs of beans (protein content 43%) 65%, corn flour 10%, wheat bran 7%, take off nuclear yeast 8%, immunity star 3%, vegetable oil 3%, salt 0.5, calcium dihydrogen phosphate 0.8%, the many ore deposits of aquatic products 0.2%, 50% Choline Chloride 0.3% is mixed with basal feed, wherein, each component in the basal feed detects respectively the indexs such as mould, bacterium, salmonella, aflatoxin by the requirement of forage health standard.Detect qualified after, pulverizer is pulverized and mixed the preparation basal feed.The lactic acid bacteria three-class strain of step (3) gained and saccharomycete three-class strain are seeded in the basal feed with 1.1% mass fraction respectively, and mix, regulating moisture to final concentration is 38%.
(5) packing
With the feed in (4) pack into settle the polybag of check valve (gas can be discharged in the bag, and bag outer gas can not enter, and can prevent entering of harmful bacteria in the air) after, enclosed package under 28 ℃, is fermented more than 16 days, can use.
Substitute traditional grass shrimp feedstuff feeding grass shrimp with this feed 5%, survival rate improves 7.3%, and per mu yield increases by 9.5%, and the grass shrimp enteritis reduces 18.2%, reduces weekly and changes water once, and the shrimp body is well-balanced, and is energetic, sells the lover.
Claims (8)
1. the preparation method of a fermented type aquatic products health-care feed is characterized in that, comprises the steps: successively
(1) first class inoculum preparation
Picking one ring saccharomycete is inoculated in the sub-culture medium of yeast solid bacterial classification from the candida utili bacterium test tube solid slant medium, under 28 ~ 35 ℃ of incubators, cultivates after 24～48 hours, makes the saccharomycete first class inoculum; Picking one ring lactic acid bacteria from the lactic acid bacteria test tube solid slant medium is inoculated into and fills in the lactic acid bacteria liquid seed culture medium triangular flask, under 35 ~ 40 ℃, leaves standstill and cultivates 24～48 hours, makes the lactic acid bacteria first class inoculum;
(2) second class inoculum preparation
The inoculum concentration access by 0.5～5% volume ratio of the saccharomycete bacterium liquid that makes in the step (1) is filled in the seed fermentation tank of the sub-culture medium of liquid yeast bacterial classification, air agitation fermentation 24～72 hours, temperature is 28～37 ℃, makes the saccharomycete second class inoculum; The lactic acid bacterial liquid that step (1) is made fills in the seed fermentation tank of the sub-culture medium of liquid lactobacillus inoculation by the inoculum concentration access of 0.5～5% volume ratio, leaves standstill and cultivates 24～48 hours, and temperature is 35～40 ℃, makes the lactic acid bacteria second class inoculum;
(3) three-class strain preparation
Inoculum concentration by 2～10% volume ratios fills the saccharomycete bacterium liquid access that makes in the step (2) in the fermentation tank of saccharomycete minimal medium, and adjusting the pH value is 6.5～7.5, and air agitation fermentation 24～72 hours makes the saccharomycete three-class strain; Inoculum concentration by 2～10% volume ratios fills the lactic acid bacterial liquid access that makes in the step (2) in the fermentation tank of lactic acid bacteria minimal medium, and adjusting the pH value is 5.5～6, stirs fermentation 24～72 hours, makes the lactic acid bacteria three-class strain;
(4) inoculation
With dregs of beans 60 ~ 75%, corn flour 10 ~ 20%, wheat bran 5 ~ 10%, take off nuclear yeast 3 ~ 10%, immunity star 1 ~ 8%, vegetable oil 1 ~ 3%, salt 0.1 ~ 0.5%, calcium dihydrogen phosphate 0.5 ~ 1%, the many ore deposits of aquatic products 0.05 ~ 0.2%, 50% Choline Chloride 0.1 ~ 0.3% is mixed with basal feed, and the lactic acid bacteria three-class strain of step (3) gained and saccharomycete three-class strain are seeded to basal feed with 0.25 ~ 2% mass fraction respectively, and mix, regulating moisture to final concentration is 34 ~ 38%;
(5) packing
With the feed in (4) pack into settle the polybag of check valve after, enclosed package, under the room temperature, spontaneous fermentation 10 ~ 25 days gets product.
2. the preparation method of fermented type aquatic products health-care feed according to claim 1, it is characterized in that, candida utili bacterium slant medium comprises following component described in the step (1): glucose 5g/L, peptone 2g/L, yeast extract 1g/L, sodium chloride 0.5g/L, agar 15g/L, the pH of saccharomycete slant medium are 6.5～7.5.
3. the preparation method of fermented type aquatic products health-care feed according to claim 1 is characterized in that, the lactic acid bacteria slant medium comprises following component described in the step (1): skimmed milk power 100.0g/L, dusty yeast 1g/L, K
2HPO
45g/L, agar 15g/L.
4. the preparation method of fermented type aquatic products health-care feed according to claim 1 is characterized in that, saccharomycete seed culture medium comprises following component described in the step (2): glucose 10 g/L, urea 1g/L, K
2HPO
40.1g/L, MgSO
47H
2O 0.1g/L, corn steep liquor 0.6g, the pH of saccharomycete seed culture medium is 6.5～7.5.
5. the preparation method of fermented type aquatic products health-care feed according to claim 1, it is characterized in that, lactic acid bacteria seed culture medium comprises following component described in the step (2): glucose 20.0g/L, peptone 10.0g/L, beef extract 10.0g/L, yeast extract 5.0g/L, anhydrous sodium acetate 5.0g/L, K
2HPO
42.0g/L, diammonium hydrogen citrate 2.0g/L, MgSO
47H
2O 0.58g/L, MnSO
4H
2O 0.33g/L, the pH of lactic acid bacteria seed culture medium is 6.0～6.5.
6. the preparation method of fermented type aquatic products health-care feed according to claim 1 is characterized in that, the saccharomycete minimal medium comprises following component described in the step (3): glucose 10g/L, urea 1g/L, K
2HPO
40.1g/L, MgSO
47H
2O 0.2g/L, corn steep liquor 0.1g/L, CaCO
33g/L, pH are 6.5～7.5.
7. the preparation method of fermented type aquatic products health-care feed according to claim 1, it is characterized in that, the lactic acid bacteria minimal medium comprises following component described in the step (3): glucose 20.0g/L, peptone 10.0g/L, beef extract 10.0g/L, yeast extract 5.0g/L, anhydrous sodium acetate 5.0g/L, dipotassium hydrogen phosphate 2.0g/L, diammonium hydrogen citrate 2.0g/L, MgSO
47H
2O 0.58g/L, MnSO4H
2O 0.33g/L, pH are 6.0～6.5.
8. the preparation method of fermented type aquatic products health-care feed according to claim 1 is characterized in that, the adjusting pH value in the step (3) adopts the NaOH solution of mass concentration 20～30% to regulate.
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| CN106889306A (en) * | 2017-02-27 | 2017-06-27 | 天津市农业生物技术研究中心 | A kind of feed fermentation agent and the application on feed fermentation |
| CN108157672A (en) * | 2017-12-12 | 2018-06-15 | 安徽希普生物科技有限公司 | A kind of production method of nonreactive fermentation fresh water fish material for having an antibacterial action and application |
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| CN103222540A (en) * | 2013-04-28 | 2013-07-31 | 河南科技大学 | Composite micro-ecological fish feed additive |
| CN103222540B (en) * | 2013-04-28 | 2014-05-07 | 河南科技大学 | Composite micro-ecological fish feed additive |
| CN104904994A (en) * | 2015-07-14 | 2015-09-16 | 谷实农牧集团股份有限公司 | Three major types of bacteria mixed liquid fermentation, solid yeast feed additive and preparation method thereof |
| CN106889306A (en) * | 2017-02-27 | 2017-06-27 | 天津市农业生物技术研究中心 | A kind of feed fermentation agent and the application on feed fermentation |
| CN108157672A (en) * | 2017-12-12 | 2018-06-15 | 安徽希普生物科技有限公司 | A kind of production method of nonreactive fermentation fresh water fish material for having an antibacterial action and application |
| CN109662208A (en) * | 2019-02-26 | 2019-04-23 | 天津九州大地饲料有限公司 | A kind of preparation process of piglet fermentative feedstuff of microbe |
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