CN104431621B - A kind of aquatic products compound premix and preparation method thereof - Google Patents
A kind of aquatic products compound premix and preparation method thereof Download PDFInfo
- Publication number
- CN104431621B CN104431621B CN201410821674.8A CN201410821674A CN104431621B CN 104431621 B CN104431621 B CN 104431621B CN 201410821674 A CN201410821674 A CN 201410821674A CN 104431621 B CN104431621 B CN 104431621B
- Authority
- CN
- China
- Prior art keywords
- parts
- compound
- extract
- seaweed bio
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The present invention relates to a kind of aquatic products compound premix, and with air dry matter amount percentage, comprising 10 15% seaweed bio activity extracts, 5 10% compound acidulants, 5 10% compound probiotic powder, remaining is carrier.The present invention also provides a kind of preparation method, including (1) by the raw material ultramicro grinding of seaweed bio activity extract, after ultrasonic microwave collaboration is extracted and filtered, filtrate is as extract solution, filter residue is digested, ferments to obtain zymotic fluid, is mixed in proportion after extract solution and zymotic fluid are freeze-dried respectively；(2) by carrier ultramicro grinding, then it is well mixed with compound acidulant, compound probiotic and seaweed bio activity extract.The premix of the present invention can effectively utilize marine algae resource, can extracellular proteinase, promote digesting and assimilating for protein, strengthen body resisting stress and anti-disease ability, the raising aquatic livestock speed of growth, so as to reduce aquaculture cost, significantly improve culture benefit.
Description
Technical field
The present invention relates to feeding additive aquatic animal, more particularly to a kind of aquatic products compound premix and preparation method thereof.
Background technology
Aquatic products are one of food being loved by people, and aquaculture brings good economic benefit to fishmen.
However, with the development of the diseases such as the deterioration of breeding environment, bacterium, aquaculture aquaculture receives serious blow in recent years.
The problems such as particularly fishes and shrimps immunity of organisms is low, growth is slow, morbidity is severe, survival rate is low, have had influence on culture fishery
Develop in a healthy way.Aquatic feeds as one of most important means of production in aquaculture (50-70% for having accounted for totle drilling cost), its
Quality parameter all has to the immune disease-resistance power of fishes and shrimps body, the speed of growth, feed coefficient, cultivation survival rate, culture benefit etc.
Important influence.
In recent years, as population sharply increases, plough and largely reduce, feedstuff is in short supply quick as aquaculture is influenceed
Development it is serious the problem of, people and animal strive the contradiction of grain also more serious.Researcher be directed to finding always it is a kind of into
This low feedstuff.Marine alga is a kind of inexpensive feed resource, and as feedstuff and feed addictive, it is opened marine alga
It is very big to send out potentiality.In addition, marine alga contains abundant algal polysaccharides, protein, fat, vitamin, mineral matter and with special
The physiological activator of effect, it is the raw material storage of food, medicine, feed, sea-plant is nutritious, contains multiple biological activities
Material, there is the bioactivity such as enhancing immunity of organisms, disease-resistant, antiviral, promotion growth.
Although marine alga has many good qualities as raw material of aquatic product feed stuff or feed addictive, the aquatic feeds in China
Sector application comes with some shortcomings, and is mainly shown as：(1) lack system to tangleweed at present in depth to study；(2) cultivation is dynamic
Thing is poor to the digestion power of algal polysaccharides, easily causes the waste of resource, and production technology is that seaweed fodder commercial development faces
A great problem；(3) composition of the nutriment of various algae and content difference are very big in tangleweed, and how scientific compatibility is reasonable
Combination, plays its special efficacy, reaches safe and efficient use, the purpose for improving breeding performonce fo animals is also a problem.
In summary, in aquatic feeds and additive agent field, finding one kind, both cost is low, and and can improves aquatic livestock and exempted from
The raw material of epidemic disease power be there is an urgent need to.Although marine alga have cost it is low, it is nutritious the characteristics of, however, at present also fail to by
Efficiently and safely it is used in aquatic livestock additive.
The content of the invention
The deficiency for more than, the present invention, which provides one kind, can effectively utilize marine algae resource so that feed cost reduces and and can
Improve a kind of aquatic products compound premix of animal survival rate and breeding efficiency and preparation method thereof.
The present invention utilizes specific marine alga composition, is specifically handled, and it is good and have and resist to obtain easy to digest, palatability
The seaweed bio activity extract of the functions such as bacterium, and coordinate with probiotics compound, acidulant compound, obtain stability it is good,
The obvious compound premix of economic value.
A kind of aquatic products compound premix, with air dry matter amount percentage, extracted comprising 10-15% seaweed bios activity
Thing, 5-10% compound acidulant, 5-10% compound probiotic powder, remaining is carrier.
According to dry measure parts by weight meter, above-mentioned seaweed bio activity extract is preferably made by following raw material：Split kettle algae 5~
10 parts, 10~20 parts of sea-tangle, 5~15 parts of undaria pinnitafida, 35~50 parts of asparagus, 15~25 parts of Enteromorpha, 5~15 parts of seaweed.
According to dry measure parts by weight meter, above-mentioned compound acidulant preferably comprise 50~70 parts of sodium butyrate, sodium Diacetate 10~
20 parts, 20~30 parts of potassium diformate.
According to dry measure parts by weight meter, above-mentioned compound probiotic powder preferably includes 30~50 parts of bacillus subtilis, acidophilus
30~50 parts of lactobacillus, 20~30 parts of clostridium butyricum, streptococcus fecalis 5-10 parts.
According to dry measure parts by weight meter, above-mentioned carrier includes 0~20 part of silica 1,30~50 parts of corncob, starch 40
~50 parts.
Above-mentioned aquatic products is with the physical and chemical index of compound premix：Character is light yellow or yellowish-brown powder；Granularity is 95-
98% passes through 200 mesh standard sieves；The uniformity is the 9.0-10.0% coefficient of variation；Moisture 7.0-8.0%.
Use weight ratio of the above-mentioned compound premix in aquatic feeds is 1-2% (mass percent).
The present invention also provides a kind of method for preparing above-mentioned aquatic products compound premix, including：
(1) preparation of seaweed bio activity extract；By the raw material ultramicro grinding of seaweed bio activity extract, ultrasound
After ripple-microwave cooperating is extracted and filtered, filtrate is digested to filter residue as extract solution, ferments to obtain zymotic fluid, by extract solution
Extract of active substances is proportionally mixed to get after being freeze-dried respectively with zymotic fluid, the ratio of mixing is preferably by dry
Weight is measured than for the extract solution after freeze-drying：Zymotic fluid=1 after freeze-drying:100；
(2) by carrier ultramicro grinding, then with compound acidulant, compound probiotic powder and seaweed bio activity extract
It is well mixed, obtain compound premix.
Preferably, the enzymolysis of the step of above-mentioned preparation method (1) is digested to add complex enzyme, and complex enzyme is by fiber
Plain enzyme, pectase, papain, the complex enzyme of zytase composition.
It is further preferred that above-mentioned complex enzyme cellulase, pectase, papain, the mass ratio of zytase
For 3:2:3:2, the mass ratio of the complex enzyme and reaction substrate is 3~4:100.
It is further preferred that the reaction condition of above-mentioned complex enzyme zymohydrolysis is preferably complex enzyme：Reaction substrate (live by seaweed bio
The raw material of property extract) mass ratio 3~4:100, reaction temperature is 45~55 DEG C, and enzymolysis time is 100~150 minutes.
It is further preferred that the reaction condition of above-mentioned complex enzyme zymohydrolysis is more preferably complex enzyme：Reaction substrate (marine alga
The raw material of bioactive extracts) mass ratio 4:100, reaction temperature is 50 DEG C, and enzymolysis time is 120 minutes.
Preferably, the ultrasonic-microwave collaboration of the step of above-mentioned preparation method (1) is extracted as the marine alga after ultramicro grinding
Ethanol-hydrogen chloride is added in bioactive extracts raw material, carries out ultrasonic-microwave collaboration extraction, cooperates with the optimum condition of extraction
For：The power of ultrasonic wave be 100W, extraction time 40min, 45 DEG C of Extracting temperature, solid-liquid ratio 1g: 3mL, microwave power 250~
400W。
Preferably, the fermentation of the step of above-mentioned preparation method (1) is that addition lactic acid bacteria and yeast are fermented after enzymolysis,
Fermentation 10-12 hours obtain zymotic fluid, and the addition of lactic acid bacteria and yeast is more preferably：Lactic acid bacteria is 100,000,000 every milliliter,
Dusty yeast：Total liquid is 1：100 (mass volume ratios).
Preferably, the commercially available purchase raw material of above-mentioned compound probiotic powder is prepared, it is preferred that voluntarily preparing, preparation method
Preferably：Freeze drying protectant is added after liquid fermentation and culture, centrifugation, concentration, vacuum freeze drying obtains compound probiotic powder.
Wherein, above-mentioned compound probiotic powder determines the work in every gram of bacterium powder according to international GB/T4789.35--2003 methods
Bacterium number, measure obtain viable count in bacterium powder and are more than 108CFU/g。
Wherein, the freeze drying protectant during prepared by above-mentioned compound probiotic powder, which forms, is preferably：Sucrose 16g, sorbierite 16g,
Skimmed milk power 19g, distilled water add to 250mL；After liquid fermentation and culture, centrifugation, concentration, the addition of the freeze drying protectant of addition
Ratio is the thalline after concentration：Freeze drying protectant is 1g:100mL, frozen-dried protective rate is up to more than 75%.
The present invention provides a kind of compound premixing of the seaweed bio activity extract compounding probiotics used in aquatic feeds
Material, digestive utilization ratio, improvement palatability, aquatic livestock can be improved after being added in aquatic feeds and is grown in rapid, feed water
Stability is good, reduce feed coefficient, improves survival rate height and culture benefit.
The beneficial effects of the present invention are：
(1) seaweed bio active material extraction is carried out using Ultrasonic-microwave synergistic extraction technology, by ultrasonic wave and microwave
Two kinds of electromagnetic wave phases combine, and make full use of the invigoration effect of ultrasonic activation and the high energy effect of microwave, overcome simple profit
With the deficiency of ultrasonic wave or microwave abstracting, realize to quick, the high efficiency extraction of marine alga active ingredient under the conditions of low-temperature atmosphere-pressure, and
And do not destroy the wherein active ingredient such as seeweed polyphenol；
(2) lactic acid bacteria employed in the zymotechnique of seaweed bio activity extract and saccharomycete, antibiont can be secreted
Plain class material, antagonism is produced to pathogenic entero becteria；Acid producing ability is strong, and lactic acid and acetic acid can be produced in enteron aisle, also simultaneously
The addition of the acidulants such as short chain fatty acids is enhanced in premix, not only improves and effectively suppresses fishes and shrimps enteron aisle common pathogen,
Promote the reparation of intestinal tract injury again；The utilization rate of calcium, phosphorus, iron can be improved, promotes the absorption of iron and vitamin D again；It can produce
Vitamin K and vitamin B, it is fishes and shrimps body replenishing vitamins；Can also extracellular proteinase, promote digesting and assimilating for protein,
Body resisting stress and anti-disease ability are enhanced, improves the speed of growth, so as to reduce aquaculture cost；
(3) the compound probiotic powder used in premix of the present invention, acid resistance is strong, be able to can not be grown in other lactic acid bacterias
Environment in grow, colonization ability is strong, i.e., a large amount of viable bacterias can smoothly enter enteron aisle and play prebiotic function；Use probiotics and acid
Agent etc. substitutes the medicated premixs such as the antibioticses growth accelerator in existing premix product, noresidue, it is pollution-free, do not produce
Drug resistance, solves the problem of pathogenic bacteria resistance to drugs is strong caused by the additives such as long-term use of antibiotic, and animal immunizing power declines；
(4) material combination of the seaweed bio activity extract of premix of the invention have passed through the compatibility checking of science,
And ultramicro grinding, enzymatic hydrolysis and fermentation PROCESS FOR TREATMENT are have passed through, degrades part SNSP, contains various abundant amino
Acid, ALANINE therein, L-Methionine, histidine, arginine, ornithine etc. has strong attractant effect to prawn, fits
Mouth property is good, and digestibility is high；It our experiments show that, the feed of compound premix of the present invention is added to fishes and shrimps feeding, palatability is good, ingests
Speed is fast, digests, and reduces molten mistake of the feed in water, the speed of growth is fast, and it is short to go out the pool time；
(5) due to the seaweed bio activity extract obtained in the present invention, there is algal polysaccharides therein raising animal to exempt from
The effect of epidemic disease power, the acrylic acid contained, terpenes, bromination phenols and some sulfur-containing compounds have antibacterial action, sulfated polysaccharide
With antivirus action；Wherein split kettle algae and enhance the high unsaturated fatty acids such as DHA, promote cranial nerve development and anti-oxidant removing
Oxygen radical ability；Feeding adds the fishes and shrimps of this compound premix feed, and anti-stress ability is strong, and immunity of organism premunition improves,
Survival rate is high, and culture benefit is notable；
(6) because the seaweed bio activity extract obtained in the present invention contains a certain amount of biological viscosity jelly,
After processing, the natural high-quality binder of feed can be turned into, the stability of fish and shrimp feed can be strengthened, prevent water quality
Pollution.
Embodiment
The present invention is further described below in conjunction with specific embodiment.
Embodiment 1
A kind of aquatic products compound premix, in terms of air dry matter parts by weight, each group is divided into：15 parts of seaweed bio activity extractions
Thing, 8 parts of compound acidulants, 10 parts of compound probiotic powder, 66 parts of carriers.
Wherein, seaweed bio activity extract is prepared by marine alga composition, and marine alga composition is：Split the weight of kettle algae 10
Part, the parts by weight of sea-tangle 10, the parts by weight of undaria pinnitafida 10, the parts by weight of asparagus 40, the parts by weight of Enteromorpha 20, the parts by weight of seaweed 10.
Wherein, compound probiotic powder calculates according to dry measure parts by weight, by 40 parts of bacillus subtilis, lactobacillus acidophilus 30
10 parts of part, 20 parts of clostridium butyricum, streptococcus fecalis compositions.
Wherein, compound acidulant calculates according to dry measure parts by weight, by 50 parts of sodium butyrate, 20 parts of sodium Diacetate, dioctyl phthalate
30 parts of compositions of potassium.
Wherein, carrier calculates according to dry measure parts by weight, is made up of 0 part of silica 1,50 parts of corncob, 40 parts of starch.
The preparation of above-mentioned aquatic products compound premix：
(1) preparation of seaweed bio activity extract：
After the raw material of above-mentioned marine alga composition is mixed after ultramicro grinding crosses 200 mesh sieves, ethanol-hydrogen chloride is added, is carried out
Ultrasonic-microwave collaboration extraction, ultrasonic power 100W, supersonic frequency 25kHz, 45 DEG C of Extracting temperature, solid-liquid ratio 1g: 3mL are micro-
Wave power 300W, extraction time 40min, then constant volume, is filtered, as extract solution, extract solution is freeze-dried to be carried filtrate
Take liquid pulvis；
Distilled water (the slag to sterilize is added into filter residue：Water=1:10 mass volume ratios), stirring forms marine alga slurries, makes
With every liter of 4mol salt acid for adjusting pH value 5.0, complex enzyme (cellulase, pectase, papain, xylan are added
Enzyme is with mass ratio 3:2:3:2 ratio mixes) digested, enzyme concentration is the mass percent of 4% substrate, 50 DEG C of constant temperature stirrings
Enzymolysis 2 hours, its cellulase (500,000 u/g), pectase (100,000 u/ grams), papain (100,000 u/ grams), xylan
Enzyme (100,000 u/ grams), temperature is improved to 95 DEG C, is kept for 15 minutes；Then 40 DEG C are cooled to, adds lactic acid bacteria (100,000,000 every milliliter)
With dusty yeast (yeast flakes dry matter：Total liquid=1：100 mass volume ratios), sealed fermenting 10-12 hours after stirring；It is cold
Jelly is dried to obtain zymotic fluid pulvis；
In dry measure ratio it is 1 by extract solution pulvis and zymotic fluid pulvis:100 mixing obtain seaweed bio activity extraction
Thing.
(2) preparation of compound probiotic powder：
By probiotics strain by liquid deep layer fermenting culture, zymotic fluid centrifuged, concentrated, add frozen-dried protective
Agent, vacuum freeze drying, obtain powdery compound probiotic powder.Every gram of bacterium powder is determined by international GB/T4789.35--2003 methods
In viable count, viable count is more than 10 in bacterium powder8CFU/g。
Wherein, the composition of freeze drying protectant is as follows：Sucrose 16g, sorbierite 16g, skimmed milk power 19g, distilled water add to
250mL；The adding proportion of thalline and freeze drying protectant after centrifugal concentrating is 1:100 ((g/mL), frozen-dried protective rate reach 75%
More than.
(3) carrier is crossed into 200 mesh sieves through ultramicro grinding, lived with compound acidulant, compound probiotic powder and seaweed bio
Property extract is well mixed, and obtains compound premix.
Embodiment 2
The culture experiment of the compound premix of embodiment 1
The compound premix of embodiment 1 is added in Penaeus monodon feed according to the ratio of mass percent 1.5% and made
For experimental group, the Penaeus monodon feed (control group) with not adding premix carries out check experiment, wherein, the spot section pair of control group
Shrimp feed, in terms of air dry matter parts by weight, each group is divided into：30 parts of fish meal, 23 parts of dregs of beans, 3 parts of shrimp shell meal, 10 parts of peanut press pulp, cotton dregs 4
Part, 4 parts of rapeseed meal, 20 parts of wheat flour, 1 part of fish oil, 1 part of soya-bean oil, 1 part of soyabean lecithin oil, 1 part of calcium dihydrogen phosphate, remaining addition
2 parts of agent.And experimental group then adds 1.5 parts of compound premixs into the Penaeus monodon feed of control group, reduce by 1.5 portions of wheat flours
Powder.
In Wenchang, hainan city Weng Tian cultivation bases, contrast test, experimental group are carried out using the feed of experimental group and control group
With control group totally 10 mouthfuls of pools, every mouthful of 3 mu of pool, every mu of 50,000 tails, totally 150 ten thousand tail, the initial galassing of Penaeus monodon are 0.26 gram every
Tail, raise 80 days respectively.
As a result show：The prawn survival rate 93.2% of experimental group, the Penaeus monodon (survival rate with feeding control group feed
78%) contrast, survival rate significantly improves, the feed coefficient (1.02) of experimental group, the egg of experimental group more lower slightly than control group (1.13)
White matter efficiency (2.23), higher than control group (2.06), the specification of the prawn of experimental group reaches average body to identical number of days (80 days) afterwards
15.8 grams are weighed per tail, and control group is 14.2 grams of every tails.Due to experimental group shrimp per ton feed cost than control group feed
Cost is low 100 yuan, and due to prawn specification is big and selling price is higher by 0.5 yuan of shrimp per jin, using every mu of experimental group raising
Culture benefit earns 300 yuan more than control group.
Embodiment 3
A kind of aquatic products compound premix, in terms of air dry matter parts by weight, each group is divided into：15 parts of seaweed bio activity extractions
Thing, 10 parts of compound acidulants, 10 parts of compound probiotic powder, 75 parts of carriers.
Wherein, seaweed bio activity extract is prepared by marine alga composition, and marine alga composition is：Split the weight of kettle algae 10
Part, the parts by weight of sea-tangle 20, the parts by weight of undaria pinnitafida 10, the parts by weight of asparagus 35, the parts by weight of Enteromorpha 20, the parts by weight of seaweed 5.
Wherein, compound probiotic powder calculates according to dry measure parts by weight, by 35 parts of bacillus subtilis, lactobacillus acidophilus 40
5 parts of part, 20 parts of clostridium butyricum, streptococcus fecalis compositions.
Wherein, compound acidulant calculates according to dry measure parts by weight, 55 parts of sodium butyrate, 15 parts of sodium Diacetate, potassium diformate
30 parts.
Wherein, carrier calculates according to dry measure parts by weight, is made up of 0 part of silica 1,40 parts of corncob, 50 parts of starch.
The preparation of above-mentioned aquatic products compound premix：
(1) preparation of seaweed bio activity extract：
After the raw material of above-mentioned marine alga composition is mixed after ultramicro grinding, ethanol-hydrogen chloride, progress ultrasonic wave-micro- are added
Ripple collaboration extraction, ultrasonic power 100W, 45 DEG C of Extracting temperature, solid-liquid ratio 1g: 3mL, microwave power 400W, extraction time 40min,
Then constant volume, filtering, for filtrate as extract solution, extract solution is freeze-dried to obtain extract solution pulvis；
Distilled water (the slag to sterilize is added into filter residue：Water=1:10 mass volume ratios), stirring forms marine alga slurries, adjusts
PH value is saved 5.0, adding complex enzyme, (cellulase, pectase, papain, zytase are with mass ratio 3:2:3:2
Ratio mixes) digested, the mass percent of the substrate of enzyme concentration 3%, 48 DEG C of constant temperature stirrings digest 2.5 hours, wherein fiber
Plain enzyme (500,000 u/g), pectase (100,000 u/ grams), papain (100,000 u/ grams), zytase (100,000 u/ grams), improve temperature
Spend to 95 DEG C, kept for 15 minutes；Then 38 DEG C are cooled to, adds lactic acid bacteria (100,000,000 every milliliter) and dusty yeast (dry：Always
Liquid=1：100 mass volume ratios), sealed fermenting 12 hours after stirring；Freeze-drying obtains zymotic fluid pulvis；
(2) preparation of compound probiotic powder：
By probiotics strain by liquid deep layer fermenting culture, zymotic fluid centrifuged, concentrated, add frozen-dried protective
Agent, vacuum freeze drying, obtain powdery compound probiotic powder.Every gram of bacterium powder is determined by international GB/T4789.35--2003 methods
In viable count, viable count is more than 10 in bacterium powder8CFU/g。
Wherein, the composition of freeze drying protectant is as follows：Sucrose 16g, sorbierite 16g, skimmed milk power 19g, distilled water add to
250mL；The adding proportion of thalline and freeze drying protectant after centrifugal concentrating is 1:100 ((g/mL), frozen-dried protective rate reach 75%
More than.
(3) carrier is mixed through ultramicro grinding with compound acidulant, compound probiotic powder and seaweed bio activity extract
Close uniformly, obtain compound premix.
Embodiment 4
The compound premix of embodiment 3 is not added in Micropterus salmoides feed as experimental group according to 1% ratio, and not
The common Micropterus salmoides feed (control group) for adding premix carries out check experiment, wherein, the common Micropterus salmoides of control group is raised
Material, in terms of air dry matter parts by weight, each component is：40 parts of fish meal, 18 parts of dregs of beans, 5 parts of tanimoto powder, 3 parts of brewer's yeast, peanut press pulp 5
Part, 20 parts of wheat flour, 1.5 parts of fish oil, 1.5 parts of soya-bean oil, 3 parts of soyabean lecithin oil, 1 part of calcium dihydrogen phosphate, remaining additive 2
Part.Wherein, experimental group is after adding 1 part of compound premix in the common Micropterus salmoides feed of above-mentioned control group, to reduce 1 part small
Wheat flour and obtain.
In Guangdong Zhong Shan Tan Zhou cultivation bases, contrast test is carried out using the feed of experimental group and control group, experimental group and
Control group totally 8 mouthfuls of pools, every mouthful of 6 mu of pool, every mu of 10,000 tails, totally 48 ten thousand tail, the initial galassing of Micropterus salmoides be 12 grams per tails, respectively
Raising 80 days.
As a result show：The survival rate 98% of experimental group is right with the Micropterus salmoides (survival rate 90%) of feeding control group feed
Than significantly improving, the feed coefficient (1.10) of experimental group is more lower slightly than control group (1.15), and experimental group is big afterwards for identical number of days (80 days)
The specification of mouth sea bass reaches 81.5 grams of average weight per tail, and control group is 75.0 grams of every tails.Due to the fish per ton of experimental group
Feed cost is lower than the feed cost of control group 70 yuan, and due to the big body colour vigor of specification is good and selling price be higher by 0.1 yuan it is every
Jin fish, every mu of the culture benefit raised using embodiment 1 earn 100 yuan more than control group.Experimental group and control group are fed big
The raising result such as the growth performance of mouth sea bass and feed conversion and morphological indexes is as shown in table 1.
Table 1：The raising results contrast of experimental group and control group
Embodiment 5
A kind of aquatic products compound premix, in terms of air dry matter parts by weight, each group is divided into：12 parts of seaweed bio activity extractions
Thing, 9 parts of compound acidulants, 10 parts of compound probiotic powder, 69 parts of carriers.
Wherein, seaweed bio activity extract is prepared by marine alga composition, and marine alga composition is：Split the weight of kettle algae 10
Part, the parts by weight of sea-tangle 15, the parts by weight of undaria pinnitafida 10, the parts by weight of asparagus 45, the parts by weight of Enteromorpha 15, the parts by weight of seaweed 5.
Wherein, compound probiotic powder calculates according to dry measure parts by weight, by 45 parts of bacillus subtilis, lactobacillus acidophilus 30
5 parts of part, 20 parts of clostridium butyricum, streptococcus fecalis compositions.
Wherein, compound acidulant calculates according to dry measure parts by weight, by 55 parts of sodium butyrate, 20 parts of sodium Diacetate, dioctyl phthalate
25 parts of compositions of potassium.
Wherein, carrier calculates according to dry measure parts by weight, is made up of 0 part of silica 1,45 parts of corncob, 45 parts of starch.
The preparation method of the compound premix and embodiment 3 are same.
Embodiment 6
The compound premix of embodiment 5 is added in Scioenops ocellatus feed as experimental group according to 1.5% ratio, with
The common Scioenops ocellatus feed (control group) for not adding premix carries out check experiment, wherein, the general American snapper of control group
Feed, in terms of air dry matter parts by weight, each component is：30 parts of fish meal, 18 parts of dregs of beans, 5 parts of tanimoto powder, 5 parts of krill meal, brewer's yeast
3 parts, 10 parts of peanut press pulp, 20 parts of wheat flour, 1.5 parts of fish oil, 1.5 parts of soya-bean oil, 3 parts of soyabean lecithin oil, 1 part of calcium dihydrogen phosphate,
2 parts of remaining additive.Experimental group is after the general American snapper feed of control group adds 1.5 parts of compound premixs, reduces 1.5
Portion wheat flour and obtain.
In Zhanjiang East Sea Island cultivation base, contrast test, experimental group are carried out using the feed of experimental group and control group
With control group totally 5 mouthfuls of pools, every mouthful of 10 mu of pool, every mu of 10,000 tails, totally 50 ten thousand tail, the initial galassing of Scioenops ocellatus be 9.4 grams per tails,
Raise 70 days respectively.
As a result show, the survival rate 100% of experimental group, it is right with the Scioenops ocellatus (survival rate 92%) of feeding control group feed
Than significantly improving, feed coefficient (0.98) is more lower slightly than control group (1.06), the Scioenops ocellatus of identical number of days (70 days) experimental group afterwards
Specification reach 72.01 grams of average weight per tail, and control group is 66.45 grams per tails.Due to the feed of the fish per ton of experimental group
Cost is lower than the feed cost of control group 60 yuan, and due to the big body colour vigor of specification is good and selling price is higher by 0.1 yuan of fish per jin,
Every mu of the culture benefit raised using experimental group earns 90 yuan more than control group.The Micropterus salmoides that experimental group and control group are fed
The raising such as growth performance and feed conversion and morphological indexes result is as shown in table 2.
Table 2：The raising results contrast of experimental group and control group
It is described above, it is only the preferable specific embodiment of the present invention, but protection scope of the present invention is not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Design is subject to equivalent substitution or change, should all cover within the scope of the present invention.
Claims (6)
1. a kind of aquatic products compound premix, it is characterised in that with air dry matter amount percentage, given birth to comprising 10-15% marine algas
Thing activity extract, 5-10% compound acidulant, 5-10% compound probiotic powder, remaining is carrier；
In terms of dry measure parts by weight, the seaweed bio activity extract is made by following raw material：Split 5~10 parts of kettle algae, sea-tangle
10~20 parts, 5~15 parts of undaria pinnitafida, 35~50 parts of asparagus, 15~25 parts of Enteromorpha, 5~15 parts of seaweed；
In terms of dry measure parts by weight, the compound acidulant is by 50~70 parts of sodium butyrate, 10~20 parts of sodium Diacetate, potassium diformate
20~30 parts of compositions；
In terms of dry measure parts by weight, the compound probiotic powder is by 30~50 parts of bacillus subtilis, lactobacillus acidophilus 30~50
Part, 20~30 parts of clostridium butyricum, streptococcus fecalis 5-10 parts composition；
In terms of dry measure parts by weight, the carrier is by 40~50 parts of 0~20 part of silica 1,30~50 parts of corncob, starch groups
Into；Wherein, the seaweed bio activity extract is prepared by the following method：
Ethanol-hydrogen chloride is added in seaweed bio activity extract raw material after ultramicro grinding, ultrasonic-microwave collaboration is carried out and carries
Take and filter, obtain filtrate and filter residue, as extract solution, the condition for cooperateing with extraction is filtrate：The power of ultrasonic wave is 100W, carried
Take time 40min, 45 DEG C of Extracting temperature, solid-liquid ratio 1g: 3mL, 250~400W of microwave power；
Carry out addition complex enzyme to filter residue to digest, complex enzyme is by cellulase, pectase, papain, xylan
The complex enzyme of enzyme composition, the reaction condition of enzymolysis is complex enzyme：Reaction substrate mass ratio 3~4:100, reaction temperature is 45~55
DEG C, enzymolysis time is 100~150 minutes, adds lactic acid bacteria after enzymolysis and yeast is fermented, and fermentation 10-12 hours obtain
Zymotic fluid；
Extract of active substances, the ratio of mixing are proportionally mixed to get after extract solution and zymotic fluid are freeze-dried respectively
Example be by dry measure weight than for freeze-drying after extract solution：Zymotic fluid=1 after freeze-drying:100.
A kind of 2. method for preparing aquatic products compound premix as claimed in claim 1, it is characterised in that including：
(1) by the raw material ultramicro grinding of seaweed bio activity extract, after ultrasonic-microwave collaboration is extracted and filtered, filtrate is made
For extract solution, filter residue is digested, ferments to obtain zymotic fluid, after extract solution and zymotic fluid are freeze-dried respectively according to
Ratio is mixed to get seaweed bio activity extract；
(2) by carrier ultramicro grinding, then mixed with compound acidulant, compound probiotic powder and seaweed bio activity extract
Uniformly, compound premix is obtained.
3. method as claimed in claim 2, it is characterised in that the compound probiotic powder be through liquid fermentation and culture, centrifugation,
Freeze drying protectant is added after concentration, vacuum freeze drying obtains compound probiotic powder.
4. method as claimed in claim 2, it is characterised in that the enzymolysis of the step (1) is digested to add complex enzyme,
Complex enzyme is the complex enzyme being made up of cellulase, pectase, papain, zytase.
5. method as claimed in claim 2, it is characterised in that the ultrasonic-microwave collaboration of the step (1) is extracted as super
Ethanol-hydrogen chloride is added in seaweed bio activity extract raw material after Crushing of Ultrafine, carries out ultrasonic-microwave collaboration extraction, it is described
The power of ultrasonic wave is 100W, extraction time 40min, 45 DEG C of Extracting temperature, solid-liquid ratio 1g: 3mL, 250~400W of microwave power.
6. method as claimed in claim 2, it is characterised in that the fermentation of the step (1) is that lactic acid bacteria is added after enzymolysis
Fermented with yeast, fermentation 10-12 hours obtain zymotic fluid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410821674.8A CN104431621B (en) | 2014-12-23 | 2014-12-23 | A kind of aquatic products compound premix and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410821674.8A CN104431621B (en) | 2014-12-23 | 2014-12-23 | A kind of aquatic products compound premix and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104431621A CN104431621A (en) | 2015-03-25 |
| CN104431621B true CN104431621B (en) | 2017-11-21 |
Family
ID=52878969
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410821674.8A Active CN104431621B (en) | 2014-12-23 | 2014-12-23 | A kind of aquatic products compound premix and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104431621B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107149053A (en) * | 2017-03-22 | 2017-09-12 | 广东粤佳饲料有限公司 | A kind of compound additive premix for promoting Penaeus monodon growth and preparation method thereof and application method |
| CN107083347A (en) * | 2017-06-12 | 2017-08-22 | 佛山市顺德区科斯力达生物科技有限公司 | Biological agent and preparation method and application |
| CN108576372A (en) * | 2017-12-29 | 2018-09-28 | 青岛百瑞吉生物工程有限公司 | A method of preparing high protein feed by raw material of cyanobacteria |
| CN109198266A (en) * | 2018-09-27 | 2019-01-15 | 清远海贝生物技术有限公司 | A kind of compound premix and its preparation method and application increasing strong fish enteron aisle |
| EP4045668A1 (en) * | 2019-10-15 | 2022-08-24 | PharmaBiome AG | Use of glycerol for increasing butyrate production by bacteria in a consortium |
| TWI745896B (en) * | 2020-03-18 | 2021-11-11 | 國立台灣海洋大學 | Sea fungus extract for enhancing fish immunity and disease resistance, its preparation method and application |
| CN112655839A (en) * | 2020-12-14 | 2021-04-16 | 珠海市德海生物科技有限公司 | Exogenous enzyme composite preparation and preparation method and application thereof |
| CN113796472A (en) * | 2021-05-18 | 2021-12-17 | 佳纬生物技术有限公司 | Traditional Chinese medicine algae compound feed for improving immunity of fingerling fingerlings and preparation method thereof |
| CN113749023B (en) * | 2021-10-10 | 2022-10-21 | 广东格新生物科技有限公司 | Seaweed hydrolysate produced by composite process and application thereof in shrimp culture |
| CN114470084A (en) * | 2022-01-06 | 2022-05-13 | 佛山市顺德区旺海饲料实业有限公司 | Environment-friendly and efficient composition for preventing and treating white feces of tilapia mossambica as well as preparation method and application thereof |
| CN114766593A (en) * | 2022-04-07 | 2022-07-22 | 中山大学 | Fermented seaweed essence and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1337169A (en) * | 2001-04-24 | 2002-02-27 | 上海众望饲料有限公司 | Production process and product of aquatic green feed without antibiotic |
| CN101263870A (en) * | 2008-03-28 | 2008-09-17 | 宜兴市天石饲料有限公司 | Highly effective biological acidulant and preparation thereof |
| CN101803682A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Compound microbial agent and production method and application thereof |
| CN101878858A (en) * | 2010-06-23 | 2010-11-10 | 南京农业大学 | Microecological composite growth-promoting feed additive for fish |
| CN103355491A (en) * | 2013-07-29 | 2013-10-23 | 中山大学 | Alga immunopotentiation compound feed for litopenaeus vannamei |
-
2014
- 2014-12-23 CN CN201410821674.8A patent/CN104431621B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1337169A (en) * | 2001-04-24 | 2002-02-27 | 上海众望饲料有限公司 | Production process and product of aquatic green feed without antibiotic |
| CN101263870A (en) * | 2008-03-28 | 2008-09-17 | 宜兴市天石饲料有限公司 | Highly effective biological acidulant and preparation thereof |
| CN101803682A (en) * | 2009-12-30 | 2010-08-18 | 沈阳科丰牧业科技有限公司 | Compound microbial agent and production method and application thereof |
| CN101878858A (en) * | 2010-06-23 | 2010-11-10 | 南京农业大学 | Microecological composite growth-promoting feed additive for fish |
| CN103355491A (en) * | 2013-07-29 | 2013-10-23 | 中山大学 | Alga immunopotentiation compound feed for litopenaeus vannamei |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104431621A (en) | 2015-03-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104431621B (en) | A kind of aquatic products compound premix and preparation method thereof | |
| CN103355491B (en) | Alga immunopotentiation compound feed for litopenaeus vannamei | |
| CN103859237B (en) | A kind of compound aquatic product micro-capsule open-mouthed bait and preparation method thereof and application | |
| CN105325719A (en) | Growth promoting fodder special for growing and fattening pigs | |
| CN111034878A (en) | Compound feed additive for improving growth performance of aquatic animals and preparation method thereof | |
| CN103704530B (en) | A kind of loach seedling special compound feed | |
| CN102648739A (en) | Litopenaeus vannamei compound feed special for winter shed cultivation and preparation method for feed | |
| CN103141701B (en) | Micro-ecological feed capable of improving intestinal function of pelteobagrus fulvidraco fries in feeding habit conversion period | |
| CN104664160B (en) | Freshwater fish composite particles preparation with physiological function and preparation method thereof | |
| CN105124230A (en) | Taihu sheep feed and production method thereof | |
| CN101569377A (en) | Shrimp feed and preparation method thereof | |
| CN101878858A (en) | Microecological composite growth-promoting feed additive for fish | |
| CN105010832A (en) | Secondary curing producing method for piglet feed | |
| CN112617055A (en) | Red swamp crayfish fish meal-free compound feed and preparation method thereof | |
| CN108432979A (en) | A kind of Australia freshwater lobster ecological feed | |
| CN106071420A (en) | The preparation method of a kind of trace element chelated peptide and Litopenaeus vannamei breeding feed | |
| CN106974134A (en) | Improve the feed addictive and its feed and preparation method of hybridized snakehead fish weed survival rate | |
| KR100857771B1 (en) | Compositions for addition to feed for fish comprising Bacillus polyfermenticus, Bacillus licheniformis and Saccharomyces serevisiae | |
| CN106720956A (en) | A kind of carp feed | |
| CN103875977A (en) | Mixed feed for aquaculture | |
| CN103493987B (en) | A kind ofly prevent and treat water-soluble complex enzyme preparation of livestock and poultry diarrhea and growth promoting effects and preparation method thereof | |
| CN105533167A (en) | Alga oligosaccharide fish feed additive | |
| CN106852416A (en) | A kind of prawn aquaculture special compound feed preparation method | |
| CN102091089A (en) | Application of active burdock oligosaccharide to aquaculture | |
| CN107897518A (en) | A kind of fish meal containing a variety of enzyme preparations |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20171019 Address after: The town of Taiping District in Guangdong Province, 511853 fresh conscience farm Qingyuan City Applicant after: Qingyuan Haibei Biological Technology Co., Ltd. Address before: 511442, No. 18, No. six, North Street, South Village, Panyu District, Guangzhou, Guangdong Applicant before: Guangdong Haid Group Co., Ltd. |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200812 Address after: 511400 Huambo four road, Panyu District, Guangzhou, Guangdong 42 No. 2 701 Patentee after: GUANGDONG HAID GROUP Co.,Ltd. Address before: The town of Taiping District in Guangdong Province, 511853 fresh conscience farm Qingyuan City Patentee before: QINGYUAN HAIBEI BIOLOGICAL TECHNOLOGY Co.,Ltd. |
|
| TR01 | Transfer of patent right |