CN105533167A - Alga oligosaccharide fish feed additive - Google Patents
Alga oligosaccharide fish feed additive Download PDFInfo
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- CN105533167A CN105533167A CN201510894666.0A CN201510894666A CN105533167A CN 105533167 A CN105533167 A CN 105533167A CN 201510894666 A CN201510894666 A CN 201510894666A CN 105533167 A CN105533167 A CN 105533167A
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Abstract
The invention discloses an alga oligosaccharide fish feed additive and relates to an alga oligosaccharide. Aiming at solving the problems of the prior art, the invention provides the alga oligosaccharide fish feed additive. The alga oligosaccharide fish feed additive improves a feed utilization rate, promotes growth, improves disease resistance, reduces a cost and improves fish quality. The alga oligosaccharide of the alga oligosaccharide fish feed additive is gracilaria lemaneiformis agar oligosaccharide and is a mixture which comprises tetrasccharide to octasaccharide and is obtained through co-culture of a Flammeovirga Pacifica H2 strain and gracilaria lemaneiformis so that gracilaria lemaneiformis is directly degraded through an enzyme produced through microorganism. The Flammeovirga Pacifica H2 has a preservation number of CCTCC NO: M2012229.
Description
Technical field
The present invention relates to a kind of alga oligosaccharides, especially relate to a kind of alga oligosaccharides from asparagus and preparing application and the method thereof of fish feed additive.
Background technology
Feed addictive refers to the material at Feed Manufacturing processing, a small amount of or micro-trophism of adding in use procedure or non-nutritive, and in feed, consumption seldom but have remarkable result.Feed addictive is the raw material that modern feed industry must use, and to strengthening basal feed nutritive value, improves breeding performonce fo animals, ensures animal health, saves feed cost, improves the aspects such as livestock products quality and have obvious effect.Therefore, the form that it may be necessary feed addictive improves fish to the utilization rate of feed, to reduce the utilization of the protein sources such as fish meal in feed, exploitation and expansion feed resource, thus improve fish survival rate, cheat disease ability, the speed of growth, food conversion degree, reduce feed cost, increase economic efficiency.80 ~ nineties of 20th century, in order to improve cultivation level and efficiency, countries in the world aquaculture all successively uses such as exogenous hormones class or antipsychotics additive in feed and drinking-water, but this type of medicated premix residue is also appeared suddenly by the harmfulness of food chain to human-body safety thereupon.Ratified to lack in the feed addictive used the kind of efficient, low toxicity, noresidue in China.Searching can substitute exogenous hormones class or antipsychotics additive, and can growth-promoting effect significantly, again to carcass noresidue, to the natural feed addictive of human body without harm, significant.
Alga oligosaccharides is the reduced sugar of the different molecular weight deriving from marine alga, because of its have improve immunity, antibacterial, antiviral, anti-ageing, improve the effects such as function of intestinal canal and receive much concern.In aquatic products, alga oligosaccharides effectively can improve gastrointestinal tract environment, promotes the growth of intestinal beneficial flora, and protection intestinal mucosa, can apply in aquaculture as growth accelerator, immunopotentiator and antiviral drugs.In external report alginic acid, contained natural antibiotics has antivirus action.Alga oligosaccharides makes raw material and is selected from natural seaweed, and have good compatibility with terrestrial plant, nontoxic to people, animal, environmentally safe, has the advantage that other additives are all incomparable.
Alga oligosaccharides has relevant report as animal fodder additive.
Chinese patent CN1843153A discloses " a kind of active alga oligosaccharide that utilizes is as the application of animal fodder additive ", described active alga oligosaccharide algin is carried out the compound sugar that a kind of molecular weight that biotechnology process obtains is less than 5000Da, has specificity unsaturated double-bond at this compound sugar non-reducing end.This activated oligosaccharide element is applied in animal feed in the scope of 1: 10000 ~ 1: 10 by oligosaccharide and feed ratio.This activated oligosaccharide element has growth promotion to animal, improves immunity, inhibited to pathogen.
The applicant provides a kind of asparagus agaropectin oligose and preparation method thereof and application in Chinese patent CN102827899A, preparation method: after 1) bacterial strain Pacific Ocean heat color bacillus (FlammeovirgaPacifica) H2 activation medium flat board being activated, be inoculated in seed culture medium, shaken cultivation obtains zymotic fluid; Described activation medium flat board is: containing the gardon asparagus powder 1% after 60 ~ 80 orders sieve, yeast extract 0.2%, NaCl3%, agar-agar powder 1.5%, and distilled water is prepared, and through 121 DEG C, uses after 20min sterilizing; Described seed culture medium is: containing CMC-Na20.5% ~ 1.5%, yeast extract 0.2%, NaCl3%, and distilled water is prepared, and through 121 DEG C, uses after 20min sterilizing; Described Pacific Ocean heat color bacillus (FlammeovirgaPacifica) H2 is preserved in China typical culture collection center on June 13rd, 2012, and deposit number is CCTCCNO:M2012229.
Summary of the invention
For the deficiencies in the prior art, technical problem to be solved by this invention is, provides the utilization rate that can improve feed, growth promoting effects, improves resistance against diseases, reduces costs, and can improve a kind of alga oligosaccharides fish feed additive of fish quality simultaneously.
The alga oligosaccharides of described alga oligosaccharides fish feed additive is asparagus agaropectin oligose, be utilize strain Pacific Ocean heat color bacillus (FlammeovirgaPacifica) H2 and an asparagus Dual culture, the enzyme system produced by Institute of Micro-biology is directly degraded asparagus preparation and the mixture of 4 ~ 8 sugar that obtains.
Described Pacific Ocean heat color bacillus (FlammeovirgaPacifica) H2 is separated voluntarily by inventor and obtains, and be preserved in China typical culture collection center on June 13rd, 2012, deposit number is CCTCCNO:M2012229, and address is Wuhan, China university (postcode 430072) (the Chinese patent CN102827899A see the applicant).The source of sample be nero deep 5378m depths mud sample (E157 ° of 24'31 ", N19 ° of 30'30 ").The screening separation of bacterial strain and cultural method are: halmeic deposit 2g is put into the 2216E fluid nutrient medium of 30mL containing 1% gardon asparagus powder of sieving through 60 ~ 80 orders, enrichment in 4 DEG C.Then pregnant solution is diluted 1000 times, coat on the 2216E flat board of the gardon asparagus powder of sieving through 60 ~ 80 orders containing 1%, in 16 DEG C of cultivations.The bacterial strain obtained is further purified conservation, and identifies.
The present invention has the following advantages and beneficial effect:
This alga oligosaccharides additive has and improves intestinal environment and promote absorption to nutriment, increases the palatability of feed, and then increases fish to the intake of feed, growth promoting effects, improve the conversion ratio of feed, improve output and the fish products matter of fish culture; Secondly, this alga oligosaccharides additive has raising animal non-specific immunity, promotes fish Cellular Immunity and humoral immunity.Especially this feed addictive edible can improve body phagocyte to the identification of foreign pathogens or foreign matter, engulf.This alga oligosaccharides additive can improve animal body humoral indications vigor simultaneously, as superoxide dismutase, peroxidase, acid phosphatase and alkaline phosphatase etc.And this alga oligosaccharides has antioxidation, there is the efficient ability removing hydroxy radical.Have very strong fungistatic effect to pathogenic bacteria such as salmonella, Vibrio vulnificus, vibrio alginolyticus, Vibrio harveyi, will Hayes vibrios, staphylococcus aureus, pseudomonas aeruginosas, in addition, this alga oligosaccharides also has antiviral activity.This alga oligosaccharides can increase the content of fish unrighted acid.
And deriving from the alga oligosaccharides of marine alga, the alga oligosaccharides especially deriving from asparagus there is not yet correlative study and patent.The present invention utilizes the alga oligosaccharides from asparagus can significantly improve efficiency of feed utilization, growth promoting effects; Secondly, method of the present invention is simple, cost is low, be easy to promotion and application; Meanwhile, the present invention's oligosaccharides used belongs to natural extract, the harm that the use avoiding exogenous hormones class additive causes human body, and free from environmental pollution.
Detailed description of the invention
The present invention is further illustrated for following examples.
One, experiment of single factor
1 experiment grouping
The Tilapia mossambica 1600 of about 50g is trailed machine and is divided into 16 groups, put 8.75m × 8.13m in a suitable place to breed, raise in the water body of 0.5 ~ 0.8m.4 groups of tests are set simultaneously, are respectively control group, low dosage (0.5g/kg) group, middle dosage (1g/kg) group, high dose (2g/kg) group, often organize test arrange 3 parallel.
2 experimental techniques
Test site select the people's police by Fujian Academy of Agricultural Sciences's chief creating build together-country (Fuqing) modern agriculture Demonstration Garden in intelligent fish cultivating system.Adopt Fujian Tianma Technology Group Co., Ltd.'s fish premixed feed by standard oneself preparation bait, then add in bait by the alga oligosaccharides of formula ratio in experiment grouping, the method that the additive that alga oligosaccharides is mixed with 1 ‰ adds is as follows:
Method one: every kg alga oligosaccharides adds 5 ~ 10g adhesive, and (main component is converted starch, Beijing fishing is through biotechnology Co., Ltd) stir, then the sterile tap water of 3 ~ 5 times of drug weight is added, stir into thick liquid, the amount being 1: 1000 by oligosaccharides weight and feed relative ratio is evenly sprayed on feed, is dried by feed or saves backup after drying.With the feed that this method obtains, alga oligosaccharides effectively can adhere to feed surface fast, and is conducive to the drying of feed.
Method two: the alga oligosaccharides taking forage volume 1 ‰ adds in basal feed, after grinding, stirring, mixing, saves backup.By the feed that this method obtains, be more used in aquatic product animal-breeding, its oligosaccharides is embedded in feed content can slow down the rate of dissolution of alga oligosaccharides in water body, thus can improve the utilization rate of alga oligosaccharides to the full extent.
3 experimental results
The speed of growth and bait conversion ratio experimental result refer to table 1.
Table 1
Two, application experiment 1
In intelligent fish cultivating system in country's (Fuqing) modern agriculture Demonstration Garden, carry out Australia dragon design spot (also known as the auspicious cod of ink, originate in the Australian Mo Rui river in the Southern Hemisphere, be the carnivorous fresh-water fishes of one that under true Percidae, wheat river blackfish belongs to) application experiment, experimental period totally 60 days.Choose body weight and be about 10g (body length is 9 ~ 10cm) fry 400 tail, be divided into experimental group and control group.By described alga oligosaccharides additive and premixed feed in 1: 1000 ratio prepare burden.In 1 month, growing of fish is significantly improved, after 2 months the development of body type of fish and wild fish very close, and very resistance to fortune, to cook taste through plain boiled water, delicious meat is tender, and commodity value greatly improves.Experimental period internal reference group fry average weight gain about 12g, survival rate 94.3%; Experimental group fry average weight gain about 18g, survival rate 97.5%; The speed of growth and survival rate are all significantly increased.
Three, application experiment 2
In Shaowu City eel culture field, experimental period totally 60 days, about choosing about 100g eel 400 tail, is divided into experimental group and control group.By described alga oligosaccharides additive and premixed feed in 1: 1000 ratio prepare burden.In 1 month, growing of fish is significantly improved, and feeding rate improves, and after 2 months, development of body type and the wild fish of fish is very close, and vigor is more glossy more by force, and to cook taste through plain boiled water, delicious meat is tender, and commodity value greatly improves.Experimental period internal reference group fry average weight gain about 60g, survival rate 95%; Experimental group fry average weight gain about 100g, survival rate 97.5%; The speed of growth and survival rate are all significantly increased.
The present invention relates to a kind of fish feed additive.The enzyme system that the alga oligosaccharides that this method relates to utilizes a strain Pacific Ocean heat color bacillus FlammeovirgaPacificaH2 to produce asparagus of directly degrading obtains, and is a kind of mixture of 4 ~ 8 sugar.This alga oligosaccharides is applied in fish meal by the present invention, is used by the scope of alga oligosaccharides and fish meal weight ratio 1:1000-2:1000.This alga oligosaccharides has and improves probiotics bacterial group structure effect in fish enteron aisle ultra microstructure, regulating intestinal canal, promotes the absorption to nutriment, significantly improves the function of fish culture output.This alga oligosaccharides has antioxidation simultaneously, has the efficient ability removing hydroxy radical.In addition this additive has raising animal non-specific immunity, and enhancement fish Cellular Immunity and humoral immunity, have antiviral and pathogen is active.Fish feed additive of the present invention; Be mixed into feed and improve efficiency of feed utilization, promote feed digestion and absorption, improve the growth rate of fish, improve fish immunity and disease resistance ability.
Claims (1)
1. an alga oligosaccharides fish feed additive, it is characterized in that the alga oligosaccharides of described alga oligosaccharides fish feed additive is asparagus agaropectin oligose, be utilize strain Pacific Ocean heat color bacillus (FlammeovirgaPacifica) H2 and an asparagus Dual culture, the enzyme system produced by Institute of Micro-biology is directly degraded asparagus preparation and the mixture of 4 ~ 8 sugar that obtains;
Described Pacific Ocean heat color bacillus (FlammeovirgaPacifica) H2 is preserved in China typical culture collection center on June 13rd, 2012, and deposit number is CCTCCNO:M2012229.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106036220A (en) * | 2016-06-16 | 2016-10-26 | 国家海洋局第三海洋研究所 | Freshwater fish feed and preparation method thereof |
| CN107373096A (en) * | 2017-08-11 | 2017-11-24 | 福建大昌生物科技实业有限公司 | Application of the agaropectin oligose in fish feed is prepared as additive |
| CN115039835A (en) * | 2022-06-24 | 2022-09-13 | 山东农业大学 | Mixed silage of sweet sorghum and sesbania as well as preparation method and application of mixed silage |
| CN116656579A (en) * | 2023-07-27 | 2023-08-29 | 海南热带海洋学院 | Novel bacterial strain for producing enzymes from ocean and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102827899A (en) * | 2012-09-18 | 2012-12-19 | 国家海洋局第三海洋研究所 | Gracilaria lemaneiformis agar oligosaccharide and preparation method and application thereof |
| CN103053806A (en) * | 2012-12-27 | 2013-04-24 | 深圳华大基因科技有限公司 | A synbiotic feed additive, and a preparation method and applications thereof |
| CN104814985A (en) * | 2015-05-13 | 2015-08-05 | 中国科学院海洋研究所 | Application of seaweed polysaccharides |
| CN104886228A (en) * | 2015-06-25 | 2015-09-09 | 集美大学 | Tilapia fillet antistaling agent and utilization method thereof |
-
2015
- 2015-12-08 CN CN201510894666.0A patent/CN105533167A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102827899A (en) * | 2012-09-18 | 2012-12-19 | 国家海洋局第三海洋研究所 | Gracilaria lemaneiformis agar oligosaccharide and preparation method and application thereof |
| CN103053806A (en) * | 2012-12-27 | 2013-04-24 | 深圳华大基因科技有限公司 | A synbiotic feed additive, and a preparation method and applications thereof |
| CN104814985A (en) * | 2015-05-13 | 2015-08-05 | 中国科学院海洋研究所 | Application of seaweed polysaccharides |
| CN104886228A (en) * | 2015-06-25 | 2015-09-09 | 集美大学 | Tilapia fillet antistaling agent and utilization method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106036220A (en) * | 2016-06-16 | 2016-10-26 | 国家海洋局第三海洋研究所 | Freshwater fish feed and preparation method thereof |
| CN107373096A (en) * | 2017-08-11 | 2017-11-24 | 福建大昌生物科技实业有限公司 | Application of the agaropectin oligose in fish feed is prepared as additive |
| CN115039835A (en) * | 2022-06-24 | 2022-09-13 | 山东农业大学 | Mixed silage of sweet sorghum and sesbania as well as preparation method and application of mixed silage |
| CN115039835B (en) * | 2022-06-24 | 2023-09-08 | 山东农业大学 | Mixed silage of sweet sorghum and sesbania as well as preparation method and application thereof |
| CN116656579A (en) * | 2023-07-27 | 2023-08-29 | 海南热带海洋学院 | Novel bacterial strain for producing enzymes from ocean and application thereof |
| CN116656579B (en) * | 2023-07-27 | 2023-10-27 | 海南热带海洋学院 | Novel bacterial strain for producing enzymes from ocean and application thereof |
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