CN103205377B - Quality bacillus coagulans for breeding broilers and application of excellent bacillus coagulans - Google Patents
Quality bacillus coagulans for breeding broilers and application of excellent bacillus coagulans Download PDFInfo
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- CN103205377B CN103205377B CN201310093952.8A CN201310093952A CN103205377B CN 103205377 B CN103205377 B CN 103205377B CN 201310093952 A CN201310093952 A CN 201310093952A CN 103205377 B CN103205377 B CN 103205377B
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Abstract
Disclosed is quality bacillus coagulans for breeding broilers. The quality bacillus coagulans is capable of regulating the immunity of the broilers, reducing the mortality rate of young livestock and poultry and improving the economical efficiency of broiler breeding. The technical scheme includes that the bacillus coagulans HM-08 is preserved in the China General Microbiological Culture Collection Center on 29th October, 2012, and the preservation number of the bacillus coagulans is CGMCC No.6728. The invention further discloses an application method for the quality bacillus coagulans.
Description
Technical field
The present invention relates to a bacillus coagulans, good Bacillus coagulans and application thereof for broiler breeding that specifically a strain can regulate body immunity, reduce cub poult mortality ratio and improve the economic benefit of broiler breeding.
Background technology
In recent years, along with the variation of social and economic condition and the raising day by day of people's living standard, people more and more pay close attention to the negative impact that uses microbiotic and pharmaceutical chemicals to bring as additive in feed, European Union forbade adding microbiotic feed from 1999, require to produce without hormone, the cry of the green livestock product of drug residue free is more and more higher, and probiotics is as the relatively more outstanding class of effect, obtain in practice very large achievement, be expected in the near future complete substitute antibiotics and be applied to production practice, finally realize real " green, healthy ", if Bacillus coagulans as fodder additives, can make our livestock products towards green future development, whole livestock industry and feed industry are had to profound significance.
The present invention is directed to above-mentioned problem, develop the good Bacillus coagulans HM-08(Bacillus coagulans HM-08 of a strain broiler breeding), as fodder additives, it can promote animal growth and development, improves efficiency of feed utilization, improves animal digestive tract micro-ecological environment, promote intestinal health, regulate body immunity, reduce cub poult mortality ratio, improve the economic benefit of broiler breeding.
Summary of the invention
Good Bacillus coagulans and the application thereof for the broiler breeding that the object of the present invention is to provide a strain can regulate body immunity, reduce cub poult mortality ratio and improve the economic benefit of broiler breeding.
The present invention is achieved through the following technical solutions.
One strain, for the good Bacillus coagulans Bacillus coagulans of broiler breeding, is characterized in that described Bacillus coagulans HM-08(Bacillus coagulans HM-08) be to screen and obtain from healthy chicken enteron aisle; This bacteria strain, on October 29th, 2012, is great Tun road, Chaoyang District, city of BeiJing, China in address, the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number: CGMCC No.6728.
An application for Bacillus coagulans, is characterized in that: for improving broiler breeding aspect.
Bacillus coagulans HM-08(Bacillus coagulans HM-08) separate in healthy chicken enteron aisle, Bacillus coagulans HM-08 can be in animal intestinal antagonism animal pathogen, promote animal growth and development, improve efficiency of feed utilization, improve animal digestive tract micro-ecological environment, promote intestinal health, regulate body immunity, reduce cub poult mortality ratio.
A kind of coagulating bacillus strain activation and cultivation, it is characterized in that: the Bacillus coagulans of freezing preservation is inoculated in GPY liquid nutrient medium, at 37 DEG C of temperature, cultivate 20h, so go down to posterity and cultivate the activated b acillus coagulans HM-08 bacterial classification described in obtaining for 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2o1.0g, K
2hPO
42.0g, MnSO
450mg, adds 1L distilled water, regulates pH to 6.8,121 DEG C of sterilizing 15min.
A preparation for Bacillus coagulans bacterium powder, is characterized in that: by the bacterial classification inoculation fermentation of above-mentioned activation, dry, pulverize and make bacterium powder, make its viable count reach 1 × 10
9cfu/g.
The using method of Bacillus coagulans in feed, is characterized in that: bacterium powder and basal diet are stirred stand-by, the addition of Bacillus coagulans HM-08 in feed is 1 × 10
5cfu/g.
Bacillus coagulans provided by the invention has following positively effect:
Experimental result shows, in feed, coagulated bacillus living number reaches 1 × 10
5when cfu/g, can make the apparent digestibility of crude protein, calcium and the phosphorus of chicken improve respectively 7.37%, 4.9% and 4.34%, and can make the water content of ight soil decline 11.09%.
Test-results shows, in feed, Bacillus coagulans HM-08 viable count reaches 1 × 10
5when cfu/g, feeding dorking can significantly reduce material anharmonic ratio, death rate, saves feed intake, improves the economic benefit of broiler production.。
Embodiment
Below in conjunction with embodiment, the invention will be further described; Following embodiment is illustrative, does not limit the scope of the invention.
Experimental technique in following embodiment, if no special instructions, is ordinary method.
One strain is for the good Bacillus coagulans Bacillus coagulans of broiler breeding, described Bacillus coagulans HM-08(Bacillus coagulans HM-08) be to screen and obtain from healthy chicken enteron aisle; This bacteria strain, on October 29th, 2012, is great Tun road, Chaoyang District, city of BeiJing, China in address, the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number: CGMCC No.6728.
An application for Bacillus coagulans, for improving broiler breeding aspect.
Bacillus coagulans HM-08(Bacillus coagulans HM-08) separate in healthy chicken enteron aisle, Bacillus coagulans HM-08 can be in animal intestinal antagonism animal pathogen, promote animal growth and development, improve efficiency of feed utilization, improve animal digestive tract micro-ecological environment, promote intestinal health, regulate body immunity, reduce cub poult mortality ratio.
A kind of coagulating bacillus strain activation and cultivating: the Bacillus coagulans of freezing preservation is inoculated in GPY liquid nutrient medium, cultivates 20h at 37 DEG C of temperature, so go down to posterity and cultivate the activated b acillus coagulans HM-08 bacterial classification described in obtaining for 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2o1.0g, K
2hPO
42.0g, MnSO
450mg, adds 1L distilled water, regulates pH to 6.8,121 DEG C of sterilizing 15min.
The preparation of Bacillus coagulans bacterium powder: by the bacterial classification inoculation fermentation of above-mentioned activation, dry, pulverize and make a bacterium powder, make its viable count reach 1 × 10
9cfu/g.
The using method of Bacillus coagulans in feed, stirs bacterium powder and basal diet stand-by, and the addition of Bacillus coagulans HM-08 in feed is 1 × 10
5cfu/g.
The application of described Bacillus coagulans aspect broiler breeding, specific experiment method is as follows:
Testing program: adopt Dan Yin to have the test method of repetition, test is divided into control group and test group, control group fed basal diet, test group is fed, and to add compound micro-ecological preparation addition in the basal diet, feed of compound micro-ecological preparation be 1g/kg.Basal diet forms in table 1.
Table 1 basal diet formula and trophic level
Index determining: measure according to a conventional method the content of crude protein (GB6435-86), calcium (GB6435-86) and phosphorus (GB6437-86) in daily ration and ight soil, calculated the apparent digestibility of crude protein, calcium and the phosphorus of daily ration by food consumption and excrement sample restatement; Calculate duration of test broiler chicken average daily gain amount, feedstuff-meat ratio and death rate.
Experimental animal management: test chicken adopts staged to raise in cages, natural ventilation, free choice feeding and drinking-water; Immunity and expelling parasite program are synchronously carried out by chicken house routine.In time receiving tank external loss material is put back in groove, observes at any time, records that chicken searches for food and healthy state.
The impact of experiment 1:Bacillus coagulans HM-08 on nutrient digestion utilization ratio in daily ration.
Select 40 of the close new AA broiler chicken of 45 age in days body weight, be divided at random 10 groups, 4 every group.Wherein 5 groups is control group, the basal diet of feeding, other 5 groups is test group, basal diet+0.1% Bacillus coagulans bacterium the powder of feeding, 45 days test periods, control group fed basal diet, test group is fed and is added the basal diet of Bacillus coagulans HM-08, and in every gram of feed, coagulated bacillus living number reaches 1 × 10
5cfu/g.Measure according to a conventional method the content of crude protein (GB6435-86), calcium (GB6435-86) and phosphorus (GB6437-86) in daily ration and ight soil, calculated the apparent digestibility of crude protein, calcium and the phosphorus of daily ration by food consumption and excrement sample restatement.Bacillus coagulans on the impact of nutrient digestion utilization ratio in daily ration in table 2.
The impact of table 2 Bacillus coagulans on nutrient digestion utilization ratio in daily ration
From table 2 test-results, in feed, Bacillus coagulans HM-08 viable count reaches 1 × 10
5cfu/g, after feeding dorking, can make the apparent digestibility of crude protein, calcium and phosphorus in chicken feed improve respectively 7.37%, 4.90% and 4.34%, and the water content of ight soil has declined 11.09% simultaneously.
Experiment 2:Bacillus coagulans HM-08 improves situation to broiler chicken day weight gain, feedstuff-meat ratio, death rate.
Select 600 of the AA broiler chicken that 1 age in days body weight is close, be divided at random 2 groups.Control group fed basal diet, test group basal diet+0.1% Bacillus coagulans bacterium the powder of feeding, 35 days test periods, control group fed basal diet, test group is fed and is added the basal diet of Bacillus coagulans, and in every gram of feed, coagulated bacillus living number reaches 1 × 10
5cfu/g.Duration of test weighs broiler weight weekly, and weigh by duplicate record food consumption, chicken and the dead number of washing in a pan every day, calculates broiler chicken average daily gain amount, duration of test feedstuff-meat ratio and death rate.Broiler chicken day weight gain, feedstuff-meat ratio and death rate improvement situation are in table 3.
Table 3 meat chicken production performance changing conditions
From table 3 test-results, in feed, Bacillus coagulans HM-08 viable count reaches 1 × 10
5cfu/g, after feeding dorking, broiler chicken average daily gain amount obviously increases compared with control group, and feedstuff-meat ratio and death rate significantly reduce.
Claims (5)
- One strain for the Bacillus coagulans of broiler breeding ( bacillus coagulans) HM-08, its preserving number is CGMCC No.6728.
- A Bacillus coagulans as claimed in claim 1 ( bacillus coagulans) application of HM-08 in the fodder additives for the preparation of broiler breeding.
- A Bacillus coagulans as claimed in claim 1 ( bacillus coagulans) activation of HM-08 and the method for cultivation, it is characterized in that: by freezing preservation bacillus coagulanshM-08 is inoculated in GPY liquid nutrient medium, at 37 DEG C of temperature, cultivates 20h, so goes down to posterity and cultivates the activation described in obtaining for 2-3 time bacillus coagulanshM-08 bacterial classification; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO 47H 2o1.0g, K 2hPO 42.0g, MnSO 450mg, adds 1L distilled water, regulates pH to 6.8,121 DEG C of sterilizing 15min.
- A Bacillus coagulans as claimed in claim 1 ( bacillus coagulans) preparation method of bacterium powder of HM-08, it is characterized in that: by the bacterial classification inoculation fermentation of activation, dry, pulverize and make bacterium powder, make its viable count reach 1 × 10 9cfu/g.
- A Bacillus coagulans as claimed in claim 1 ( bacillus coagulans) using method of HM-08 in feed, it is characterized in that: the bacterium powder after strain fermentation, freeze-drying, pulverizing and basal diet are stirred stand-by, in feed, coagulated bacillus living number reaches 1 × 10 5cfu/g.
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Cited By (1)
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| EP3281531A4 (en) * | 2015-04-06 | 2019-03-06 | Asahi Calpis Wellness Co., Ltd. | Method for improving feed conversion ratio of poultry and method for rearing poultry |
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| CN106520614A (en) * | 2016-11-18 | 2017-03-22 | 北京农学院 | Method for improving production performance of Beijing fatty chicken by using neutral protease-producing bacillus coagulans |
| CN112322524A (en) * | 2020-10-29 | 2021-02-05 | 青岛普罗百世生物科技有限公司 | Lactobacillus plantarum and application thereof in broiler feed |
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| EP3281531A4 (en) * | 2015-04-06 | 2019-03-06 | Asahi Calpis Wellness Co., Ltd. | Method for improving feed conversion ratio of poultry and method for rearing poultry |
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