CN103202400B - Composite microecological feed additive capable of improving producing capability of broiler chicken egg breed chicken - Google Patents
Composite microecological feed additive capable of improving producing capability of broiler chicken egg breed chicken Download PDFInfo
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- CN103202400B CN103202400B CN201310093656.8A CN201310093656A CN103202400B CN 103202400 B CN103202400 B CN 103202400B CN 201310093656 A CN201310093656 A CN 201310093656A CN 103202400 B CN103202400 B CN 103202400B
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Abstract
The invention discloses composite microecological feed additive capable of improving producing capability of broiler chicken egg breed chicken. The technical scheme includes that that composite microecological feed additive is characterized by comprising one strain of Lactobacillus casei HM-10 (L.casei HM-10), one strain of lactobacillus plantarum HM-20 (L.plantarum HM-20) and one strain of bacillus licheniformis (Bacillus licheniformis BL-08). Each gram of the microecological feed additive comprises, by mass, 10% of the L.casei HM-10, 10% of the L.plantarum HM-20, 10% of the Bacillus licheniformis BL-08, and 70% of zeolite powder, wherein the viable count in the L.casei HM-10 is larger than or equal to 2.5x108, the viable count in the L.plantarum HM-20 is larger than or equal to 2.5x108, and the viable count in the Bacillus licheniformis BL-08 is larger than or equal to 2x108.
Description
Technical field
The present invention relates to and belong to compound micro-ecological preparation field, especially a kind of can improve laying rate of laying hen and increase the improvement broiler chicken of broiler weight, the compound micro-ecological preparation of laying breed production performance.
Background technology
For a long time, people adopt always to add in feed antibiosis usually control broiler chicken, laying breed disease and improve production performance.Due to antibiotic a large amount of use, the quality of chicken and egg is declined, brings potential hazard to human health.Therefore, European Union forbade adding antibiotic in feed from 1999.For this reason, can nontoxic, the noresidue probiotics of substitute antibiotics develop rapidly.
Probiotics (Microecological feed additives) is referred to and can also be balanced by adjustment animal intestinal micro-ecology by Direct-fed animal, improve animal body immunity to reach prevent disease, promotion growth of animal, improve efficiency of feed utilization, the normal flora improving host health level and metabolite thereof, the living microbe formulation of selective promotion host normal flora.
The present invention is directed to above-mentioned problem, develop the compound micro-ecological preparation of a kind of Novel dry Lactobacillus paracasei (L.casei HM-10), Lactobacillus plantarum (L.plantarum HM-20) and bacillus licheniformis (Bacillus licheniformis BL-08), can be good at suppressing pathogen to grow after feeding dorking and laying hen, improve intestinal beneficial flora balance, prevent disease, effectively can improve broiler chicken and laying breed production performance.
Summary of the invention
The object of the present invention is to provide a kind of can improve laying rate of laying hen and increase the improvement broiler chicken of broiler weight, the compound micro-ecological preparation of laying breed production performance.
The present invention is achieved through the following technical solutions;
One improves broiler chicken, the compound micro-ecological preparation of laying breed production performance, it is characterized in that: described compound micro-ecological preparation is by a Lactobacillus casei HM-10 (L.casei HM-10), one lactobacillus plantarum HM-20(L.plantarum HM-20) and a bacillus licheniformis (Bacillus licheniformis BL-08) composition, in every gram of probiotics, L.casei HM-10 mass fraction is 10%, L.plantarum HM-20 mass fraction is 10%, Bacillus licheniformis BL-08 mass fraction 10%, all the other 70% are zeolite powder, L.casei HM-10 number of viable>=2.5 × 10 in every gram of probiotics
8, L.plantarum HM-20 number of viable>=2.5 × 10
8, Bacillus licheniformis BL-08 number of viable>=2 × 10
8,
Wherein, described Lactobacillus casei L.casei HM-10 is separated from healthy chicken enteron aisle, can improve animal body immunocompetence, has excellent acidproof, bile tolerance and intestinal colonisation ability, promotes that egg fowl intestinal villi grows, improve vaccine valence, Lactobacillus plantarum L.plantarum HM-20 is separated from spontaneous fermentation feed, can suppress enteropahtogenic microganism, has good acidproof, bile tolerance and intestinal colonisation ability, bacillus licheniformis Bacillus licheniformis BL-08 is separated from healthy chicken enteron aisle, it is a strain high proteinase yield, amylase, the bacterial strain of lipase and cellulase, have and maintain enteron aisle anaerobic environment, balance with lactic acid bacteria symbiosis, improve efficiency of feed utilization, above three strain bacterial strains have been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address is: Datun Road, Chaoyang District, Beijing City, Lactobacillus casei L.casei HM-10 preserving number is CGMCC No6737, preservation date is on October 29th, 2012, Lactobacillus plantarum L.plantarum HM-20 preserving number is: CGMCC No6744, preservation date is on October 29th, 2012, the preserving number of bacillus licheniformis Bacillus licheniformis BL-08 is: CGMCC No6726, preservation date is on October 29th, 2012.
Described compound micro-ecological preparation improves the effect of broiler chicken, performance in layers, and concrete grammar is as follows:
Testing program: adopt single factor test to have the test method of repetition, test is divided into control group and test group, control group fed basal diet, test group feed add compound micro-ecological preparation basal diet, compound micro-ecological preparation addition is 1g/kg in feed.
Index determining: every day is added up weight of laying eggs, egg number, feed intake, chicken number respectively, the superseded chicken wherein because pecking anus and sickness influence does not participate in data statistics; Weekly record is carried out to broiler weight, analyzing and processing is carried out to experimental data, the situation of change of comparative test group and each index of control group.
Compound micro-ecological preparation provided by the invention has following good effect:
Compound micro-ecological preparation has obvious effect for raising laying rate of laying hen aspect, 64 laying hens feed of feeding continuously containing 1g/kg probiotics 8 weeks, and laying rate statistics is: control group 79.53%, test group 83.26%; Feedstuff-egg ratio aspect: test group is 2.38, control group is 2.46; Feed intake aspect: control group is 43.92kg/week, test group is 48.40kg/week, and laying rate and feed intake are improved, and feedstuff-egg ratio decreases simultaneously, improves the economic benefit of broiler breeding.
Compound micro-ecological preparation has the effect strengthening broiler weight, and the feed of feeding containing 1g/kg probiotics is after 49 days, and broiler chicken average weight every day improves 128.9 grams, and feedstuff-meat ratio obviously reduces simultaneously.
Compound micro-ecological preparation has the effect strengthening broiler weight, the feed of feeding containing 1g/kg probiotics is after 49 days, improve the ratio of Bifidobacterium in Intestine of Broiler content, reduce colibacillary ratio simultaneously, there is the effect of good regulating intestinal canal flora.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described; Following embodiment is illustrative, does not limit the scope of the invention.
Experimental technique in following embodiment, if no special instructions, is conventional method.
One improves broiler chicken, the compound micro-ecological preparation of laying breed production performance, described compound micro-ecological preparation is by a Lactobacillus casei HM-10 (L.casei HM-10), one lactobacillus plantarum HM-20(L.plantarum HM-20) and a bacillus licheniformis (Bacillus licheniformis BL-08) composition, in every gram of probiotics, L.casei HM-10 mass fraction is 10%, L.plantarum HM-20 mass fraction is 10%, Bacillus licheniformis BL-08 mass fraction 10%, all the other 70% are zeolite powder, L.casei HM-10 number of viable>=2.5 × 10 in every gram of probiotics
8, L.plantarum HM-20 number of viable>=2.5 × 10
8, Bacillus licheniformis BL-08 number of viable>=2 × 10
8,
Wherein, described Lactobacillus casei L.casei HM-10 is separated from healthy chicken enteron aisle, can improve animal body immunocompetence, has excellent acidproof, bile tolerance and intestinal colonisation ability, promotes that egg fowl intestinal villi grows, improve vaccine valence, Lactobacillus plantarum L.plantarum HM-20 is separated from spontaneous fermentation feed, can suppress enteropahtogenic microganism, has good acidproof, bile tolerance and intestinal colonisation ability, bacillus licheniformis Bacillus licheniformis BL-08 is separated from healthy chicken enteron aisle, it is a strain high proteinase yield, amylase, the bacterial strain of lipase and cellulase, have and maintain enteron aisle anaerobic environment, balance with lactic acid bacteria symbiosis, improve efficiency of feed utilization, above three strain bacterial strains have been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, Lactobacillus casei L.casei HM-10 preserving number is CGMCC No6737, preservation date is on October 29th, 2012, Lactobacillus plantarum L.plantarum HM-20 preserving number is: CGMCC No6744, preservation date is on October 29th, 2012, the preserving number of bacillus licheniformis Bacillus licheniformis BL-08 is: CGMCC No6726, preservation date is on October 29th, 2012.
Experiment 1:
Compound micro-ecological preparation is to the improvement result of laying rate of laying hen aspect.
Select the blue brown laying breed in the sea of 202 ages in days to test, control group fed does not add the basal feed of probiotics, and test group is fed the basal feed containing 1g/kg compound micro-ecological preparation, and often organize 4 repetitions, the basic chicken quantity of each repetition is 16.Raise in the hen house of semi open model, She Nei can make full use of sunlight, gravity-flow ventilation, and day feeds 3 times, and free drink running water, test group is consistent with control group environmental condition, 8 weeks test periods.Basal diet formula and trophic level are in table 1.
Table 1 basal diet formula and trophic level
Every day entry test group, control group egg size, feed intake, egg number, carry out statistical analysis to duration of test average egg weight, feed intake, feedstuff-egg ratio, laying rate, result of the test sees the following form 2.
Table 2 compound micro-ecological preparation is on the impact of laying breed production performance
From experimental result, feed compound micro-ecological preparation continuously after 8 weeks, laying hen feed intake, laying rate significantly improves; Feedstuff-egg ratio also decreases, and this compound micro-ecological preparation has the effect of good improvement performance in layers.
Experiment 2:
Compound micro-ecological preparation is to the effect of meat chicken production performance.
Select the AA Broiler chicks 600 of 1 age in days only to test, be divided into 2 groups at random, often organize 300, the average weight difference of each group is remarkable, control group fed basal feed, and test group is fed the basal feed containing 1g/kg compound micro-ecological preparation, test period 49 days.Basal diet and trophic level are in table 3.
Weigh respectively in 14 ages in days, 35 ages in days and 49 ages in days, add up feed consumption rate and feedstuff-meat ratio, carry out analyzing and processing to the data obtained, result of the test is in table 4.
Table 3 basal diet and trophic level
Table 4 compound micro-ecological preparation is on the impact of meat chicken production performance
Tested from table 4: from on-test (the 1st day) to off-test (49 days), test group every broiler chicken average weight comparatively control group increases by 128.9 grams, feedstuff-meat ratio obviously reduces simultaneously, illustrate that compound micro-ecological preparation has strengthen and improve Broiler chicks digestion power, improve the effect of production performance.
Experiment 3:
Compound micro-ecological preparation is on Bifidobacterium in broiler chicken ight soil and colibacillary impact.
Test method is with embodiment 2, and control group and test group in 14 ages in days and the sampling of 35 ages in days, often organize random selecting 10 chickens simultaneously, adopts extruding anus method to collect ight soil in sterilized petri dishes, carries out count of bacteria with mode of averaging.Bifidobacterium adopts improved BS medium, 37 DEG C of Anaerobic culturel 48 hours; Escherichia coli adopt SS agar medium, cultivate 24 hours for 37 DEG C.Compound micro-ecological preparation on Bifidobacterium in broiler chicken ight soil and colibacillary impact in table 5.
Table 5 compound micro-ecological preparation is on Bifidobacterium in broiler chicken ight soil and colibacillary impact
From table 5 result of the test: compound micro-ecological preparation improves the ratio of Bifidobacterium in Intestine of Broiler content, reduce colibacillary ratio simultaneously, there is the effect of good regulating intestinal canal flora.
Claims (1)
1. one kind is improved broiler chicken, the compound micro-ecological preparation of laying breed production performance, it is characterized in that: described compound micro-ecological preparation is by a strain Lactobacillus casei HM-10 (L.casei HM-10), one lactobacillus plantarum HM-20(L.plantarum HM-20) and a bacillus licheniformis BL-08(Bacillus licheniformis BL-08) composition, in every gram of probiotics, L.casei HM-10 mass fraction is 10%, L.plantarum HM-20 mass fraction is 10%, Bacillus licheniformis BL-08 mass fraction 10%, all the other 70% are zeolite powder, L.casei HM-10 number of viable>=2.5 × 10 in every gram of probiotics
8, L.plantarum HM-20 number of viable>=2.5 × 10
8, Bacillus licheniformis BL-08 number of viable>=2 × 10
8,
Wherein, described Lactobacillus casei L.casei HM-10 is separated from healthy chicken enteron aisle, can improve animal body immunocompetence, has excellent acidproof, bile tolerance and intestinal colonisation ability, promotes that egg fowl intestinal villi grows, improve vaccine valence, Lactobacillus plantarum L.plantarum HM-20 is separated from spontaneous fermentation feed, can suppress enteropahtogenic microganism, has good acidproof, bile tolerance and intestinal colonisation ability, bacillus licheniformis Bacillus licheniformis BL-08 is separated from healthy chicken enteron aisle, it is a strain high proteinase yield, amylase, the bacterial strain of lipase and cellulase, enteron aisle anaerobic environment can be maintained, balance with lactic acid bacteria symbiosis, improve efficiency of feed utilization, above three strain bacterial strains have been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, Lactobacillus casei L.casei HM-10 preserving number is CGMCC No6737, preservation date is on October 29th, 2012, Lactobacillus plantarum L.plantarum HM-20 preserving number is: CGMCC No6744, preservation date is on October 29th, 2012, the preserving number of bacillus licheniformis Bacillus licheniformis BL-08 is: CGMCC No6726, preservation date is on October 29th, 2012.
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| CN103421713B (en) * | 2013-07-29 | 2016-02-10 | 内蒙古和美科盛生物技术有限公司 | For plant lactobacillus and the subtilis of the probiotic solid fermentation of feed additive field |
| CN103421711B (en) * | 2013-07-29 | 2016-02-10 | 内蒙古和美科盛生物技术有限公司 | For the lactobacterium casei of fodder additives probiotic solid fermentation, animal bifidobacteria, plant lactobacillus and subtilis |
| CN103636973B (en) * | 2013-11-27 | 2015-07-01 | 章俊 | Laying hen nutritional feed and preparation method thereof |
| CN103911324B (en) * | 2014-03-28 | 2016-05-25 | 内蒙古和美科盛生物技术有限公司 | Contain probiotics preparation of Lactobacillus plantarum and bacillus licheniformis and preparation method thereof |
| CN103911325B (en) * | 2014-03-28 | 2016-04-27 | 内蒙古和美科盛生物技术有限公司 | Bacillus coagulans, lactobacterium casei, plant lactobacillus probiotic agent and preparation |
| CN103952346B (en) * | 2014-04-17 | 2016-08-31 | 青岛九和宜生生物科技有限公司 | Lactobacillus plantarum, lactobacillus casei and the preparation of Bacillus licheniformis composite bacteria agent capable |
| CN104651279A (en) * | 2015-02-12 | 2015-05-27 | 青岛九和宜生生物科技有限公司 | Probiotic lactobacillus plantarum for improving chicken nutrient digestibility and application of probiotic lactobacillus plantarum |
| CN105062916B (en) * | 2015-07-22 | 2017-12-12 | 江南大学 | Application of one bacillus coagulans on lifting laying hen egg yield |
| CN107006677A (en) * | 2017-04-27 | 2017-08-04 | 齐齐哈尔大学 | A kind of feed and its application rich in probiotics and active peptide |
| CN108850653B (en) * | 2018-08-21 | 2021-07-09 | 山东省农业科学院家禽研究所（山东省无特定病原鸡研究中心） | Feed additive for improving reproductive performance of breeding ducks |
| CN111607542B (en) * | 2020-06-11 | 2022-07-26 | 武汉市农业科学院 | Microecological preparation and preparation method and application thereof |
| CN113789281B (en) * | 2021-09-18 | 2023-04-14 | 江苏农林职业技术学院 | Probiotics for improving egg laying performance and egg quality and application thereof |
| CN113892563B (en) * | 2021-09-18 | 2023-08-25 | 江苏农林职业技术学院 | Composite probiotic preparation for relieving cold stress |
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