CN103243043B - One strain is for improving excellent Bacillus coagulans and the application thereof of the cultivation of inferior piglet - Google Patents
One strain is for improving excellent Bacillus coagulans and the application thereof of the cultivation of inferior piglet Download PDFInfo
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Abstract
One strain can improve piglet healthy state, the excellent Bacillus coagulans that cultivates for inferior piglet of production performance of raising inferior piglet cultivation.Technical scheme is: described Bacillus coagulans HM-09(Bacillus coagulans HM-09) on October 29th, 2012, in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number: CGMCC No.6729.The invention also discloses its application method.
Description
Technical field
The present invention relates to a bacillus coagulans, specifically a strain can improve piglet healthy state, raising inferior piglet cultivation production performance for improve inferior piglet cultivation excellent Bacillus coagulans and application.
Background technology
Piglet is born from parent, its living environment and obtain the approach of nutrition and will experience a very large change, this concerning newborn piglet be one very large stress, most piglet just can reform of nature environment birth about 10 days, well-grown; And part piglet adaptive faculty is poor, easily produce disease, growth retardation, individual slight of stature, need the culturing time extending inferior piglet, or all will the many input costs of more first-class piglet to the whole breeding process becoming pig to deliver for sale, thus reduce the economic benefit of pig farm cultivation.Utilize probiotics promote weanling pig, growing and fattening pigs and pregnant sow report more, and the report that probiotics cultivates inferior piglet is little.
The present invention is directed to above-mentioned problem, develop a kind of excellent Bacillus coagulans HM-09 (Bacillus coagulans HM-09) for improving the cultivation of inferior piglet, to feed inferior piglet as fodder additives, the healthy state of piglet can be improved, increase hair color brightness, promote that ight soil is shaping, reduce stool odor, improve the production performance of inferior piglet cultivation.
Summary of the invention
The object of the present invention is to provide a strain can improve piglet healthy state, the excellent Bacillus coagulans that cultivates for sow of the production performance of raising inferior piglet cultivation and application.
The present invention is achieved through the following technical solutions.
One strain is for improving the Bacillus coagulans of inferior piglet cultivation, it is characterized in that: described Bacillus coagulans HM-09 (Bacillus coagulans HM-09) is on October 29th, 2012, it is great Tun road, Chaoyang District, city of BeiJing, China in address, the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number: CGMCC No.6729.
An application for Bacillus coagulans, is characterized in that: described Bacillus coagulans HM-09 (Bacillus coagulans HM-09) is improving the application in inferior piglet cultivation.
Bacillus coagulans HM-09 (Bacillus coagulans HM-09) is separated in health pig enteron aisle, and Bacillus coagulans HM-09 can improve inferior piglet hair color luminance brightness, healthy state, promotes stool forming simultaneously, reduces stink.。
A kind of activation of coagulating bacillus strain and cultivation, it is characterized in that: the Bacillus coagulans HM-09 of freezen protective is inoculated in GPY liquid nutrient medium, at temperature 37 DEG C, cultivate 20h, Secondary Culture like this obtains described activated b acillus coagulans HM-09 bacterial classification for 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2o 1.0g, K
2hPO
42.0g, MnSO
450mg, adds 1L distilled water, regulates pH to 6.8,121 DEG C of sterilizing 15min.
A kind of Bacillus coagulans using method in feed, it is characterized in that: stir stand-by by the bacterium powder after strain fermentation, freeze-drying, pulverizing and basal diet, the addition of Bacillus coagulans in feed is 1 × 10
5cfu/g.
Bacillus coagulans provided by the invention has following positively effect:
Experimental result shows, in inferior piglet feed, add 1 × 10
5the Bacillus coagulansHM-09 of cfu/g, effectively can improve the average daily gain of inferior piglet, reduce feedstuff-meat ratio simultaneously.
Test-results shows, in inferior piglet feed, add 1 × 10
5the Bacillus coagulansHM-09 of cfu/g, effectively can improve the luminance brightness of inferior piglet hair color, healthy state and the shaping situation of ight soil.
Embodiment
Below in conjunction with embodiment, the invention will be further described; Following embodiment is illustrative, does not limit the scope of the invention.
Experimental technique in following embodiment, if no special instructions, is ordinary method.
One strain is for improving the Bacillus coagulans of inferior piglet cultivation, described Bacillus coagulans HM-09 (Bacillus coagulans HM-09) is on October 29th, 2012, it is great Tun road, Chaoyang District, city of BeiJing, China in address, the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number: CGMCC No.6729.
An application for Bacillus coagulans, Bacillus coagulans HM-09 (Bacillus coagulansHM-09) is improving the application in inferior piglet cultivation.
Bacillus coagulans HM-09 (Bacillus coagulans HM-09) is separated in health pig enteron aisle, and Bacillus coagulans HM-09 can improve inferior piglet hair color luminance brightness, healthy state, promotes stool forming simultaneously, reduces stink.。
A kind of activation of coagulating bacillus strain and cultivation, the Bacillus coagulansHM-09 of freezen protective is inoculated in GPY liquid nutrient medium, at temperature 37 DEG C, cultivate 20h, Secondary Culture like this obtains described activated b acillus coagulans HM-09 bacterial classification for 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2o 1.0g, K
2hPO
42.0g, MnSO
450mg, adds 1L distilled water, regulates pH to 6.8,121 DEG C of sterilizing 15min.
A kind of Bacillus coagulans using method in feed, stir stand-by by the bacterium powder after strain fermentation, freeze-drying, pulverizing and basal diet, the addition of Bacillus coagulans in feed is 1 × 10
5cfu/g.
Bacillus coagulans HM-09 is improving the application in inferior piglet, and concrete grammar is as follows:
Testing program: select the tri-crossbreeding 20 that growing way is poor, every piglet body weight is at 13-17kg, piglet is weighed on an empty stomach, people for being divided into two groups, body weight come first 10 for control group, body weight come latter 10 be test group, often organize 10 piglets, control group fed basal diet, test group is fed basal diet+0.1%Bacillus coagulans HM-09 bacterium powder, 30 days test periods.Basal diet composition and trophic level are in table 1.
Table 1 basal diet composition and trophic level
Index determining: measure piglet empty body weight before and after test, feed consumption rate, observes piglet healthy state, the shaping situation of ight soil; Testing data according to record adds up the average daily ingestion amount of piglet, average daily gain and feed-weight ratio.
Experimental animal manages: test piglet adopts semi-open pig house, natural ventilation, piglet free choice feeding, and nipple water dispenser is freely drunk water; Immunity and expelling parasite program are undertaken by pig farm conventional synchronous.Timely receiving tank external loss material is put back in groove, observes at any time, records and search for food and healthy state.
Experiment 1:Bacillus coagulans HM-09 is to the improvement of inferior piglet production performance.
Select the tri-crossbreeding 20 that growing way is poor, every piglet body weight is at 13-17kg, piglet is weighed on an empty stomach, people is for being divided into two groups, body weight come first 10 for control group, body weight come latter 10 be test group, often organize 10 piglets, control group fed basal diet, test group is fed coagulated bacillus living number>=1 × 10
5the basal diet of cfu/g, 30 days test periods.Situation is improved in table 2 to inferior piglet production performance.
The inferior piglet production performance of table 2 improves situation
Note: above data are the mean value of 10 inferior piglets.
From table 2 test-results, inferior piglet is fed Bacillus coagulans HM-09 viable count>=1 × 10
5the basal diet of cfu/g 30 days, the average food consumption of test group and average adding weight are apparently higher than control group, feed-weight ratio also obviously reduces, and the nearly control group of last reclosing during test group off-test, illustrates that Bacilluscoagulans HM-09 is improved the effect of the production performance of inferior piglet.
Experiment 2:Bacillus coagulans HM-09 is to the improvement situation of inferior piglet hair color and ight soil.
To piglet tracing observation every day healthy state, the hair color changing conditions of control group and test group in embodiment 1, the shaping and stink changing conditions of record ight soil.Inferior piglet hair color and ight soil improve situation in table 3.
The healthy state of the inferior piglet of table 3 duration of test improves situation
From table 3 test-results, the hair color brightness of test group inferior piglet during off-test, the shaping situation of ight soil and healthy state are significantly better than control group, and pig place stink test group is also significantly better than control group.
Claims (2)
1. Bacillus coagulans (Bacillus coagulans) HM-09 improves the application of inferior piglet feed ingredient in preparation, and described Bacillus coagulans is separated to obtain from health pig enteron aisle; This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number: CGMCC No.6729, and preservation date is on October 29th, 2012.
2. apply as claimed in claim 1, wherein the method for Bacillus coagulans activation and cultivation is: be inoculated in GPY liquid nutrient medium by Bacillus coagulans (Bacillus coagulans) HM-09 of freezen protective, at temperature 37 DEG C, cultivate 20h, Secondary Culture like this obtains described activation Bacillus coagulans (Bacillus coagulans) HM-09 bacterial classification for 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2o 1.0g, K
2hPO
42.0g, MnSO
450mg, adds 1L distilled water, regulates pH to 6.8,121 DEG C of sterilizing 15min.
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| CN103243043B true CN103243043B (en) | 2015-09-16 |
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| CN103911326B (en) * | 2014-03-28 | 2016-08-10 | 内蒙古和美科盛生物技术有限公司 | The preparation of Bacillus coagulans probiotics preparation |
| CN103911328B (en) * | 2014-03-28 | 2017-01-18 | 内蒙古和美科盛生物技术有限公司 | Preparation method of bacillus coagulans and lactobacillus casei compound bacterial preparation |
| CN104017751B (en) * | 2014-04-17 | 2017-03-29 | 青岛九和宜生生物科技有限公司 | Bacillus coagulans, Lactobacillus plantarum and Lactobacillus casei preparation and its preparation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250817A (en) * | 2011-07-21 | 2011-11-23 | 北京金泰得生物科技股份有限公司 | Bacillus coagulans for feeding and application thereof |
| CN103255082A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding finishing pigs and application thereof |
| CN103255080A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding piglets and application thereof |
| CN103255081A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding sows and application thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250817A (en) * | 2011-07-21 | 2011-11-23 | 北京金泰得生物科技股份有限公司 | Bacillus coagulans for feeding and application thereof |
| CN103255082A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding finishing pigs and application thereof |
| CN103255080A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding piglets and application thereof |
| CN103255081A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding sows and application thereof |
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