CN103255082A - Excellent bacillus coagulans for breeding finishing pigs and application thereof - Google Patents
Excellent bacillus coagulans for breeding finishing pigs and application thereof Download PDFInfo
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- CN103255082A CN103255082A CN2013100934596A CN201310093459A CN103255082A CN 103255082 A CN103255082 A CN 103255082A CN 2013100934596 A CN2013100934596 A CN 2013100934596A CN 201310093459 A CN201310093459 A CN 201310093459A CN 103255082 A CN103255082 A CN 103255082A
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Abstract
The invention discloses an excellent bacillus coagulans which is used in breeding finishing pigs and has effects of maintaining and adjusting microecological balance, enhancing body immunologic function and helping finishing pigs digest and absorb a feed and an application thereof. The technical scheme is characterized in that the bacillus coagulans HM-09 is preserved in the China General Microbiological Culture Collection Center on Oct. 29th, 2012; and the collection number is CGMCC No. 6729. The invention also discloses an application method of the excellent bacillus coagulans.
Description
Technical field
The present invention relates to a bacillus coagulans, specifically is that a strain is kept and microecosystem regulation balance, enhancing body immunologic function and the promotion growing and fattening pigs Bacillus coagulans and the application thereof that are used for the growing and fattening pigs breed of digesting and assimilating to feed.
Background technology
Along with improving constantly of constant development of economy and standard of living, people more and more recognize the basic meaning of " green ".But because the driving of economic interests, antibiotic use has begun to spread unchecked, and particularly uses in a large number in feed, the formation that causes normal microflora imbalance in the animal body and suppress organism immune response, and the serious superinfection that causes, even can cause death; Secondly, microbiotic residual in the livestock and poultry body and in the food such as meat, egg, milk can not make its deactivation fully through heat treated, and the people is edible this based food for a long time, may cause chronic poisoning etc.If in feed, use probiotics, will reduce antibiotic consumption greatly, solve the problem that microbiotic can't resolve, enhance productivity, advance to the direction of green agriculture.If Bacillus coagulans as fodder additives, can make our livestock products develop towards green direction, whole livestock industry and feed already there is profound significance.
The present invention is directed to above-mentioned problem, develop the good Bacillus coagulans HM-09(Clostridium butyricum HM-09 that a kind of growing and fattening pigs are cultured), feed after the growing and fattening pigs as fodder additives, can be in the growing and fattening pigs enteron aisle antagonism animal pathogen, keep and the microecosystem regulation balance, the enhancing body immunologic function promotes growing and fattening pigs digesting and assimilating feed, improve day weight gain, treatment is had loose bowels etc.
Summary of the invention
The object of the present invention is to provide a strain to keep and microecosystem regulation balance, enhancing body immunologic function and the promotion growing and fattening pigs good Bacillus coagulans and the application thereof that are used for the growing and fattening pigs breed of digesting and assimilating to feed.
The present invention is achieved through the following technical solutions.
One strain is used for the good Bacillus coagulans that growing and fattening pigs are cultured, it is characterized in that described Bacillus coagulans HM-09(Bacillus coagulans HM-09) on October 29th, 2012, be No. 13, one in north, Zhong Guan-cun, Haidian District, BeiJing, China city in the address, common micro-organisms DSMZ of China Committee for Culture Collection of Microorganisms (State Patent Office specifies patent microbial preservation center) preservation, preserving number: CGMCC No.6729.
A kind of application of Bacillus coagulans is characterized in that: the application of described Bacillus coagulans aspect the growing and fattening pigs breed.
Bacillus coagulans HM-09(Clostridium butyricum HM-09) separates in the health pig enteron aisle, can be in animal intestinal the antagonism animal pathogen, keep and the microecosystem regulation balance, strengthen the immunologic function of animal, promote growing and fattening pigs digesting and assimilating feed, improve day weight gain, treatment is had loose bowels etc.
A kind of activation of Bacillus coagulans and cultivation, it is characterized in that: the Clostridium butyricum HM-09 of freezing preservation is inoculated in the GPY liquid nutrient medium, cultivate 20h down for 37 ℃ in temperature, so going down to posterity to cultivate obtains described activation Clostridium butyricum HM-09 bacterial classification 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2O1.0g, K
2HPO
42.0g, MnSO
450mg adds 1L distilled water, regulates pH to 6.8,121 ℃ of sterilization 15min.
The using method of a kind of Bacillus coagulans in feed is characterized in that: the bacterium powder after strain fermentation, freeze-drying, the pulverizing and basal diet are stirred stand-by, the addition of Clostridium butyricum HM-09 in feed is 1 * 10
5Cfu/g.
Bacillus coagulans provided by the invention has following positively effect:
Experimental result shows, adds 1 * 10 in fattening pannage
5The Bacillus coagulans of cfu/g, at the different growth phases of test, the day weight gain effect of growing and fattening pigs is obvious, has improved the economic benefit that growing and fattening pigs are produced.
Test-results shows, adds 1 * 10 in fattening pannage
5The Bacillus coagulans of cfu/g can reduce the incidence that growing and fattening pigs are suffered from diarrhoea, and can reduce the generation of intestines source property disease simultaneously, improves feed digestibility and utilization ratio.
Embodiment
The invention will be further described below in conjunction with embodiment; Following embodiment is illustrative, does not limit protection scope of the present invention.
One strain is used for the good Bacillus coagulans that growing and fattening pigs are cultured, it is characterized in that described Bacillus coagulans HM-09(Clostridium butyricum HM-09) on October 29th, 2012, be No. 13, one in north, Zhong Guan-cun, Haidian District, BeiJing, China city in the address, common micro-organisms DSMZ of China Committee for Culture Collection of Microorganisms (State Patent Office specifies patent microbial preservation center) preservation, preserving number: CGMCC No.6729.
A kind of application of Bacillus coagulans is characterized in that: the application of described Bacillus coagulans aspect the growing and fattening pigs breed.
Bacillus coagulans HM-09(Clostridium butyricum HM-09) separates in the health pig enteron aisle, can be in animal intestinal the antagonism animal pathogen, keep and the microecosystem regulation balance, strengthen the immunologic function of animal, promote growing and fattening pigs digesting and assimilating feed, improve day weight gain, treatment is had loose bowels etc.
A kind of activation of Bacillus coagulans and cultivation, it is characterized in that: the Clostridium butyricum HM-09 of freezing preservation is inoculated in the GPY liquid nutrient medium, cultivate 20h down for 37 ℃ in temperature, so going down to posterity to cultivate obtains described activation Clostridium butyricum HM-09 bacterial classification 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2O1.0g, K
2HPO
42.0g, MnSO
450mg adds 1L distilled water, regulates pH to 6.8,121 ℃ of sterilization 15min.
The using method of a kind of Bacillus coagulans in feed is characterized in that: the bacterium powder after strain fermentation, freeze-drying, the pulverizing and basal diet are stirred stand-by, the addition of Clostridium butyricum HM-09 in feed is 1 * 10
5Cfu/g.
Experimental technique among the following embodiment if no special instructions, is ordinary method.
The application of described Bacillus coagulans aspect the weanling pig production performance, concrete grammar is as follows:
Testing program: the selection kind is growing and fattening pigs in vain long and Large White hybridization, and this test branch fattens early stage (20-50Kg) and fatten the later stage carry out in (50-90Kg) two stages synchronously.Fatten early stage and choose and be in a good state of health, body weight is neat, and growing environment is similar fattens 64 of piglets, is divided into two groups at random, and wherein test group is 33, and the coagulated bacillus living number is 1 * 10 in the feed
5Cfu/g, 31 of control groups, the basal diet of feeding, test period is 60 days; Fattening the later stage, to choose body weight neat, is in a good state of health, and growing environment is similar, body weight is fattened 58 of piglets about 50KG, is divided into two groups at random, 30 of test group, and the coagulated bacillus living number is 1 * 10 in the feed
5Cfu/g, 28 of control groups, the basal diet of feeding, test period is 50 days.Basal diet is formed and trophic level sees Table 1.
Table 1 basal diet is formed and trophic level is seen
Index determining: average daily ingestion amount: accurate weighing every day and record test group and control group feeding coal, the average daily ingestion amount of every piglet is calculated in weighing and record clout amount during off-test; Average daily gain: on-test and off-test 8 titles in morning piglet are heavy on an empty stomach, the average daily gain of calculating every piglet; Feedstuff-meat ratio: calculate test group and control group feedstuff-meat ratio according to average daily ingestion amount and average daily gain; Diarrhea rate: observe piglet ight soil situation every day after on-test, record test group and control group diarrhoea piglet head, and a last number of times that diarrhoea takes place with every group of piglet calculates diarrhea rate divided by total raising number of times.
The experimental animal management: the test piglet adopts high bed to raise, semi-open pig house, and natural ventilation, the piglet free choice feeding, the nipple water dispenser is freely drunk water; Immunity and expelling parasite program are carried out synchronously by the pig farm routine.In time receiving tank external loss material is put back in the groove, observes at any time, records and search for food, healthy state and pig house environment.
Experiment 1: Bacillus coagulans HM-09(Clostridium butyricum HM-09) fattening the influence of early stage to the growing and fattening pigs production performance.
Select kind for long white growing and fattening pigs (20-50Kg) of hybridizing with Large White, be in a good state of health, body weight is neat, 64 of the similar growing and fattening pigs of growing environment.Be divided into two groups at random, wherein test group is 33, and the coagulated bacillus living number is 1 * 10 in the feed
5Cfu/g; 31 of control groups, the basal diet of feeding, test period is 60 days.
Control group fed basal diet, test group are fed and are added the basal diet of Bacillus coagulans, coagulated bacillus living number 〉=1 * 10 in the feed
5Cfu/g.Fatten in earlier stage average single head growing and fattening pigs day weight gain situation and see Table 2, feed consumption rate and feedstuff-meat ratio see Table 3.
Table 2 is fattened average single head growing and fattening pigs production performance improvement in early stage situation
Annotate: same letter representation difference is (P〉0.05) not significantly, different letter representation significant differences (P＜0.05)
Table 3 is fattened early stage and is fattened later stage feed consumption rate and feedstuff-meat ratio
By table 2 test-results as can be known, add Bacillus coagulans in the daily ration, fatten early stage growing-finishing pigs speed and significantly improve (P＜0.05) than control group, the average daily gain amount is 50 grams.
By table 3 test-results as can be known, add Bacillus coagulans in the daily ration, fatten average every the pig feed consumption rate of pre-stage test group and control group duration of test and do not have noticeable change, the test group feedstuff-meat ratio obviously reduces than control group.
Experiment 2: Bacillus coagulans HM-09(Clostridium butyricum HM-09) fattening the influence of later stage to the growing and fattening pigs production performance.
Select kind to be the growing and fattening pigs of long white and Large White hybridization, fatten the later stage and test that to choose body weight neat, be in a good state of health, growing environment is similar, body weight is fattened 58 of piglets about 50KG, be divided into two groups at random, 30 of test group, the coagulated bacillus living number is 1 * 10 in the feed
5Cfu/g, 28 of control groups, the basal diet of feeding, test period is 50 days.
Control group fed basal diet, test group are fed and are added the basal diet of Bacillus coagulans, coagulated bacillus living number 〉=1 * 10 in the feed
5Cfu/g.Fatten later stage average single head growing and fattening pigs production performance improvement situation and see Table 4, fatten early stage and fatten the later stage feed consumption rate and feedstuff-meat ratio sees Table 5.
Table 4 is fattened average single head growing and fattening pigs of later stage production performance and is improved situation
Annotate: same letter representation difference is (P〉0.05) not significantly, different letter representation significant differences (P＜0.05)
Table 5 is fattened early stage and is fattened later stage feed consumption rate and feedstuff-meat ratio
By table 4 test-results as can be known, add Bacillus coagulans in the daily ration, fatten later stage growing-finishing pigs speed and significantly improve (P＜0.05) than control group, the average daily gain amount is 90 grams.
By table 5 test-results as can be known, add Bacillus coagulans in the daily ration, fatten later stage test group every pig feed consumption rate, feedstuff-meat ratio and obviously reduce than control group.
Experiment 3: Bacillus coagulans HM-09(Clostridium butyricum HM-09) to the improvement situation of growing and fattening pigs diarrhoea and disease.
Select kind to be growing and fattening pigs in vain long and Large White hybridization, it is neat to choose body weight, be in a good state of health, growing environment is similar, body weight is fattened 64 of piglets about 20KG, is divided into two groups at random, 33 of test group, the coagulated bacillus living number is 1 * 105cfu/g in the feed, 31 of control groups, the basal diet of feeding, test period is 50 days.
Control group fed basal diet, test group are fed and are added the basal diet of Bacillus coagulans, coagulated bacillus living number 〉=1 * 10 in the feed
5Cfu/g.Bacillus coagulans sees Table 6 to the improvement situation of growing and fattening pigs diarrhoea and disease.
Table 6 Bacillus coagulans is to the improvement situation of growing and fattening pigs diarrhoea and disease.
By table 6 test-results as can be known, feed and contain coagulated bacillus living number 〉=1 * 10
5The feed of cfu/g is after 10 days, and diarrhoea and paratyphoid are sick all cures.
Experiment 4: add Bacillus coagulans HM-09(Clostridium butyricum HM-09 in the feed) to the influence of growing and fattening pigs culturing economic benefit.
Testing data with embodiment 1 and embodiment 2 is foundation, analyzes with the economic benefit of fattening later stage test group and control group fattening pre-stage test group and control group, and statistics sees Table 7.
Profit=the total augment weight of test group (kg) * pork price (unit/kilogram)-total feed consumption (kg) * feed price (unit/kilogram)-total feed consumption (kg) * Bacillus coagulans bacterium powder price (unit/kilogram)
Profit=the total augment weight of control group (kg) * pork price (unit/kilogram)-total feed consumption (kg) * feed price (unit/kilogram)
Test profit=2745 yuan of 1 test group; Control group profit=2147 yuan differ from 598 yuan
Test profit=2402 yuan of 2 test group; Control group profit=1265 yuan differ from 1137 yuan
The table 7 different tests stage is utilized analytical table
By table 7 statistics as can be known, feeding contains the feed of Bacillus coagulans, fattens 597 yuan of profit raisings in early stage, fattens the later stage profit and improves 1137 yuan, can obviously improve the economic benefit that growing and fattening pigs are cultured.
Claims (4)
1. a strain is used for the good Bacillus coagulans that growing and fattening pigs are cultured, and it is characterized in that: be to separate to obtain from the health pig enteron aisle described Bacillus coagulans HM-09(Bacillus coagulans HM-09); This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number: CGMCC No.6729, preservation date are on October 29th, 2012.
2. the application of a Bacillus coagulans is characterized in that: the application of described Bacillus coagulans aspect the growing and fattening pigs breed.
3. the activation of a Bacillus coagulans and cultivation, it is characterized in that: the Bacillus coagulans HM-09 of freezing preservation is inoculated in the GPY liquid nutrient medium, cultivate 20h down for 37 ℃ in temperature, so going down to posterity to cultivate obtains described activated b acillus coagulans HM-09 bacterial classification 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2O1.0g, K
2HPO
42.0g, MnSO
450mg adds 1L distilled water, regulates pH to 6.8,121 ℃ of sterilization 15min.
4. the using method of a Bacillus coagulans in feed is characterized in that: the bacterium powder after strain fermentation, freeze-drying, the pulverizing and basal diet are stirred stand-by, the coagulated bacillus living number reaches 1 * 10 in the feed
5Cfu/g.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103243043A (en) * | 2013-03-22 | 2013-08-14 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for improving inferior piglet breeding and application of bacillus coagulans |
| CN106138113A (en) * | 2016-08-08 | 2016-11-23 | 大连三仪动物药品有限公司 | The biological compound preparation of preventing and treating livestock and poultry diarrhea |
| CN108201014A (en) * | 2017-12-14 | 2018-06-26 | 广东乐将生物科技有限公司 | A kind of complex microorganism additive and its application |
| WO2019205422A1 (en) * | 2018-04-24 | 2019-10-31 | 广东怡和科洁科技有限公司 | Bacillus coagulans xp and application thereof in animal feed production |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1058134A (en) * | 1990-07-18 | 1992-01-29 | 宛吉斌 | Biological insecticides |
| CN1089442A (en) * | 1993-01-03 | 1994-07-20 | 四川农业大学 | The microorganism fodder additive Manufacturing approach and use |
| CN102505003A (en) * | 2011-12-19 | 2012-06-20 | 湖南省微生物研究所 | High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof |
| CN103255081A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding sows and application thereof |
-
2013
- 2013-03-22 CN CN201310093459.6A patent/CN103255082B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1058134A (en) * | 1990-07-18 | 1992-01-29 | 宛吉斌 | Biological insecticides |
| CN1089442A (en) * | 1993-01-03 | 1994-07-20 | 四川农业大学 | The microorganism fodder additive Manufacturing approach and use |
| CN102505003A (en) * | 2011-12-19 | 2012-06-20 | 湖南省微生物研究所 | High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof |
| CN103255081A (en) * | 2013-03-22 | 2013-08-21 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for breeding sows and application thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103243043A (en) * | 2013-03-22 | 2013-08-14 | 内蒙古和美科盛生物技术有限公司 | Excellent bacillus coagulans for improving inferior piglet breeding and application of bacillus coagulans |
| CN103243043B (en) * | 2013-03-22 | 2015-09-16 | 内蒙古和美科盛生物技术有限公司 | One strain is for improving excellent Bacillus coagulans and the application thereof of the cultivation of inferior piglet |
| CN106138113A (en) * | 2016-08-08 | 2016-11-23 | 大连三仪动物药品有限公司 | The biological compound preparation of preventing and treating livestock and poultry diarrhea |
| CN108201014A (en) * | 2017-12-14 | 2018-06-26 | 广东乐将生物科技有限公司 | A kind of complex microorganism additive and its application |
| CN108201014B (en) * | 2017-12-14 | 2021-08-06 | 郭升程 | Composite microbial additive and application thereof |
| WO2019205422A1 (en) * | 2018-04-24 | 2019-10-31 | 广东怡和科洁科技有限公司 | Bacillus coagulans xp and application thereof in animal feed production |
| CN110396480A (en) * | 2018-04-24 | 2019-11-01 | 广东怡和科洁科技有限公司 | A kind of bacillus coagulans XP and its application in Feed Manufacturing |
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