CN108201014B - Composite microbial additive and application thereof - Google Patents

Composite microbial additive and application thereof Download PDF

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CN108201014B
CN108201014B CN201711337691.4A CN201711337691A CN108201014B CN 108201014 B CN108201014 B CN 108201014B CN 201711337691 A CN201711337691 A CN 201711337691A CN 108201014 B CN108201014 B CN 108201014B
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mass
percentage
additive
pork
pig feed
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CN108201014A (en
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马兴寿
郭升程
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Li Zhenwei
Zhang Dongxing
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/30Feeding-stuffs specially adapted for particular animals for swines
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/02Breeding vertebrates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/26Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A23K10/28Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin from waste dairy products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Birds (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Fodder In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides a compound microbial additive and application thereof, wherein the compound microbial additive comprises 90-70% of marine bacillus N6-2 and 10-30% of bacillus coagulans in percentage by mass, and the compound microbial additive is applied to pig feed and can play a role in improving the content of intramuscular fat in pork, so that snowflake pork is obtained, and the obtained pork has higher market value.

Description

Composite microbial additive and application thereof
Technical Field
The invention relates to the technical field of feed additives, in particular to a compound microbial additive and an application thereof.
Background
The snowflake meat is a noun for showing the meat quality, because fat is deposited among muscle fibers, the snowflake meat forms obvious marble patterns with red muscle fibers, is exactly like the snowflake and is famous, the meat quality of the snowflake meat is not fat, not greasy and not firewood when being eaten, is healthy and delicious, and therefore the snowflake meat is popular with consumers and is regarded as a treasure of the meat quality. Many people know that beef has snowflake beef, but few people know that pork also has snowflake pork. The reason is that under normal conditions, obvious snowflakes are formed only when the content of intramuscular fat in pork is more than 8%, obvious fog-like snowflakes are formed only when the content of intramuscular fat in the pork reaches more than 10%, and generally, the content of intramuscular fat in the pork is between 2 and 6%, and at the moment, marbling is not obvious, and obvious snowflake-like snowflakes are not formed. So the snowflake pork is difficult to obtain and is rarely known. It is common for the skilled person to add additives to the feed to regulate the growth and development of pigs. However, no feed additive capable of improving the pork quality to obtain snowflake pork exists in the prior art.
Disclosure of Invention
The invention aims to overcome the defects of the existing feed additive and provide the compound microbial additive, and the compound microbial additive is applied to the pig feed additive to improve the quality of pork and play a role in increasing the intramuscular fat content of the pork, so that the snowflake pork is obtained, and the obtained pork has higher market value.
Another purpose of the invention is to provide the application of the composite microbial additive.
It is still another object of the present invention to provide a swine feed comprising the complex microbial additive.
It is yet another object of the present invention to provide a method for improving the quality of pork.
In order to achieve the purpose of the invention, the invention adopts the technical scheme that:
a composite microbial additive comprises 90-70% of marine bacillus N6-2 and 10-30% of bacillus coagulans in percentage by mass.
Preferably, the bacillus coagulans strain consists of 80 mass percent of bacillus marinus N6-2 and 20 mass percent of bacillus coagulans.
Wherein the marine Bacillus N6-2 is the existing known strain, is classified and named as Bacillus sp.N6-2, is sourced from China center for type culture Collection, has the preservation number of CCTCC NO: M2014586, and has the preservation address of Wuhan university at Lojia mountain of Wuchang city, China.
The inventor of the patent finds that the application of the compound microbial additive containing the bacillus marinus N6-2 and the bacillus coagulans in the pig feed additive can improve the pork quality, promote fat accumulation in muscles, play a role in increasing the intramuscular fat content in pork and obtain the snowflake pork through animal feeding experiments.
Preferably, the bacillus coagulans is bacillus coagulans HM-09, and the bacillus coagulans HM-09 is also an existing known strain and is derived from the China general microbiological culture Collection center (CGMCC), with the preservation number: CGMCC No.6729, with the preservation address of Datun road of the sunward area in Beijing of China.
Therefore, the application of the compound microbial additive in the preparation of the pig feed additive is within the protection scope of the invention.
The invention is applicable to the general pig species, preferably, but not limited to, the species swine is Hainan.
Preferably, the pig feed additive is a feed additive capable of improving the quality of pork. Preferably, the pork is pork ham.
Specifically, the pork quality is improved by enabling the intramuscular fat content of the pork to be more than or equal to 8%. In addition, more specifically, the improvement of the pork quality also refers to the increase of the protein content of the pork. Preferably, the myoglobin in the pork is increased, the higher the concentration of the myoglobin is, the redder the pork is, and the snowflake pork can be highlighted by commonly called amber red.
In addition, a pig feed additive and a pig feed containing the compound microbial additive are also within the protection scope of the invention.
The pig feed is prepared from the following raw materials in percentage by mass: 2-6% of the compound microbial additive, 80-90% of a protein source raw material, 5-16% of a fat source raw material and 2-5% of compound vitamin.
The preparation method of the pig feed can refer to the following steps:
s1, preparing a culture medium, carrying out expanded culture on the marine bacillus N6-2 and the bacillus coagulans in the composite microbial additive, and then carrying out solid fermentation, air drying, crushing and mixing to obtain the composite microbial additive;
s2, mixing the compound microbial additive with the protein source raw material, the fat source raw material and the compound vitamin according to the proportion, and sieving after air drying to obtain the pig feed.
Preferably, the pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microorganism additive, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Preferably, the protein source raw material consists of fish meal, black soldier fly larva powder and whey protein powder.
Preferably, the fish meal: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 1-2: 1 to 5.
More preferably, the fish meal: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4.
the vitamin complex generally comprises the following components in percentage by weight: vitamin B10.025wt%, riboflavin 0.1wt%, vitamin B60.075wt% of hydrochloride, 0.25wt% of nicotinic acid, 39.9wt% of inositol, 0.5wt% of calcium pantothenate, 0.25wt% of biotin, 0.025wt% of folic acid, and vitamin K30.5wt%, vitamin B12 0.25wt%, vitamin A acetate 0.25wt%, vitamin D3 0.625wt%, vitamin E acetate 7.25wt%, and medium cellulose 50 wt%.
The fat source material is animal oil such as beef tallow and lard, preferably lard.
In addition, the invention also provides a method for improving the quality of pork, and the pig feed provided by the invention is used for feeding pigs. Specifically, the method comprises the following steps: the pig feed is adopted to feed pigs of four weeks to five weeks for at least 180 days.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides the compound microbial additive, discloses the application of the compound microbial additive as a pig feed additive for the first time, and can play a role in improving the intramuscular fat content of pork when being applied to pig feed, so that snowflake pork is obtained, and the obtained pork has higher market value.
Detailed Description
The present invention will be further described with reference to the following embodiments.
The present invention is further illustrated in detail by the following specific examples, it being understood that the specific examples described herein are for the purpose of illustration only and are not intended to limit the invention, and that various changes may be made within the scope of the invention as defined by the claims. The test methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials.
The marine bacillus N6-2 and the bacillus coagulans HM-09 used in the implementation are the existing known strains. Wherein the marine bacillus N6-2 is from China center for type culture Collection, the preservation number is CCTCC NO: M2014586, and the preservation address is Wuhan university at Wuchang Lojia mountain in Wuhan City, China. The bacillus coagulans HM-09 is derived from the China general microbiological culture Collection center of the Committee for culture Collection of microorganisms with the collection number: CGMCC No.6729, with the preservation address of Datun road of the sunward area in Beijing of China.
Example 1
The pig feed is prepared from the following raw materials in percentage by mass: 2% of compound microorganism additive, 85% of protein source raw material, 10% of fat source raw material and 3% of compound vitamin.
Wherein the composite microbial additive consists of 80 percent of marine bacillus N6-2 and 20 percent of bacillus coagulans HM-09 in percentage by mass.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil.
The vitamin complex comprises the following components in percentage by weight: vitamin B10.025wt%, riboflavin 0.1wt%, vitamin B60.075wt% of hydrochloride, 0.25wt% of nicotinic acid, 39.9wt% of inositol, 0.5wt% of calcium pantothenate, 0.25wt% of biotin, 0.025wt% of folic acid, and vitamin K30.5wt%, vitamin B12 0.25wt%, vitamin A acetate 0.25wt%, vitamin D3 0.625wt%, vitamin E acetate 7.25wt%, and medium cellulose 50 wt%.
The pig feed is prepared by the following steps:
s1, preparing a culture medium, carrying out expanded culture on Bacillus marinus N6-2 and Bacillus coagulans HM-09 in the composite microbial additive, and then carrying out solid fermentation, air drying and crushing to obtain the composite microbial additive;
s2, mixing the compound microbial additive with the protein source raw material, the fat source raw material and the compound vitamin according to the proportion, and sieving after air drying to obtain the compound microbial additive.
Example 2
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microorganism additive, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the composite microbial additive consists of 80 percent of marine bacillus N6-2 and 20 percent of bacillus coagulans HM-09 in percentage by mass.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Example 3
The pig feed is prepared from the following raw materials in percentage by mass: 6% of compound microbial additive, 80% of protein source raw material, 10% of fat source raw material and 4% of compound vitamin.
Wherein the composite microbial additive consists of 80 percent of marine bacillus N6-2 and 20 percent of bacillus coagulans HM-09 in percentage by mass.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Example 4
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microorganism additive, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the composite microbial additive consists of 80 percent of marine bacillus N6-2 and 20 percent of bacillus coagulans HM-09 in percentage by mass.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 1: 1. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Example 5
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microorganism additive, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the composite microbial additive consists of 80 percent of marine bacillus N6-2 and 20 percent of bacillus coagulans HM-09 in percentage by mass.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 5. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Example 6
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microorganism additive, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the composite microbial additive consists of 80 percent of marine bacillus N6-2 and 20 percent of bacillus coagulans HM-09 in percentage by mass.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 1. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Example 7
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microorganism additive, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the composite microbial additive consists of 90 percent of marine bacillus N6-2 and 10 percent of bacillus coagulans HM-09 in percentage by mass.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Example 8
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microorganism additive, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the composite microbial additive comprises 70 percent of marine bacillus N6-2 and 30 percent of bacillus coagulans HM-09 in percentage by mass.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Comparative example 1
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microorganism additive, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the composite microbial additive consists of 80 percent of marine bacillus N6-2 and 20 percent of bacillus coagulans HM-09 in percentage by mass. The protein source raw materials only comprise fish meal and whey protein powder, wherein the fish meal: the whey protein powder is prepared from the following components in percentage by mass: 4. The fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Comparative example 2
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microorganism additive, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the composite microbial additive consists of 80 percent of marine bacillus N6-2 and 20 percent of bacillus coagulans HM-09 in percentage by mass. The protein source raw materials are soybean meal and whey protein powder, wherein the soybean meal: the whey protein powder is prepared from the following components in percentage by mass: 4. The fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Comparative example 3
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microorganism additive, 65% of protein source raw material, 28% of fat source raw material and 2% of compound vitamin.
Wherein the composite microbial additive consists of 80 percent of marine bacillus N6-2 and 20 percent of bacillus coagulans HM-09 in percentage by mass. Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Comparative example 4
The pig feed is prepared from the following raw materials in percentage by mass: bacillus coagulans HM-095%, protein source raw material 83%, fat source raw material 10%, and vitamin complex 2%.
Wherein, the fish meal in the protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Test detection
In order to research the effect of the microbial feed additive, the swine species are Hainan pigs which are selected as test objects, and the test conditions are as follows: 130 pigs at five weeks of age were selected and randomized into 13 groups of 10 pigs each. 12 of the pig feeds are prepared by the above examples and comparative examples, and the rest is a blank control example (pig feed without microbial feed additive is adopted) and is fed normally for 180 days.
Thereafter, the pork hind legs (same site) were taken, and their properties were observed and tested for nutritional components, and the results are shown in tables 1 and 2:
TABLE 1 appearance Properties
Figure DEST_PATH_IMAGE002
TABLE 2 test results
Figure DEST_PATH_IMAGE004
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims (7)

1. The application of the compound microbial additive in the preparation of the pig feed additive is characterized in that the compound microbial additive consists of 90-70 percent of marine bacillus N6-2 and 10-30 percent of bacillus coagulans in percentage by mass; the pig feed additive is a feed additive capable of improving the quality of pork; the pork quality improvement means that the intramuscular fat content of the pork is more than or equal to 8 percent.
2. The use of claim 1, wherein the composite microbial additive is composed of 80% by mass of bacillus marinus N6-2 and 20% by mass of bacillus coagulans.
3. The pig feed is characterized by being prepared from the following raw materials in percentage by mass: 2-6% of the compound microbial additive of claim 1 or 2, 80-90% of protein source raw material, 5-16% of fat source raw material and 2-5% of compound vitamin.
4. The pig feed according to claim 3, which is prepared from the following raw materials in percentage by mass: 5% of a compound microbial additive, 83% of a protein source raw material, 10% of a fat source raw material and 2% of compound vitamin.
5. The pig feed according to claim 3 or 4, wherein the protein source material consists of fish meal, black soldier fly larva meal, whey protein powder.
6. The pig feed according to claim 5, characterized in that the fish meal: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 1-2: 1 to 5.
7. A method for improving pork quality, which comprises feeding a pig with the pig feed according to any one of claims 3 to 6.
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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102090535A (en) * 2010-12-23 2011-06-15 菏泽和美华饲料有限公司 Feed for improving pig growth
CN102578412A (en) * 2012-02-17 2012-07-18 山东省农业科学院畜牧兽医研究所 Feed additive for increasing content of intramuscular fat of external ternary pigs and using method for feed additive
CN103255082A (en) * 2013-03-22 2013-08-21 内蒙古和美科盛生物技术有限公司 Excellent bacillus coagulans for breeding finishing pigs and application thereof
CN106011113A (en) * 2016-04-28 2016-10-12 中国水产科学研究院黄海水产研究所 Beta-glucanase generated by Bacillus marinus and preparation method thereof
CN106615792A (en) * 2016-12-14 2017-05-10 安佑生物科技集团股份有限公司 High digestibility shelled black soldier fly insect powder-containing piglet care feed
CN107373037A (en) * 2017-09-06 2017-11-24 四川御咖食品有限公司 A kind of biological feedstuff and complete diet pellet for the black pig in more than 30kg Sichuan-Tibet

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102090535A (en) * 2010-12-23 2011-06-15 菏泽和美华饲料有限公司 Feed for improving pig growth
CN102578412A (en) * 2012-02-17 2012-07-18 山东省农业科学院畜牧兽医研究所 Feed additive for increasing content of intramuscular fat of external ternary pigs and using method for feed additive
CN103255082A (en) * 2013-03-22 2013-08-21 内蒙古和美科盛生物技术有限公司 Excellent bacillus coagulans for breeding finishing pigs and application thereof
CN106011113A (en) * 2016-04-28 2016-10-12 中国水产科学研究院黄海水产研究所 Beta-glucanase generated by Bacillus marinus and preparation method thereof
CN106615792A (en) * 2016-12-14 2017-05-10 安佑生物科技集团股份有限公司 High digestibility shelled black soldier fly insect powder-containing piglet care feed
CN107373037A (en) * 2017-09-06 2017-11-24 四川御咖食品有限公司 A kind of biological feedstuff and complete diet pellet for the black pig in more than 30kg Sichuan-Tibet

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