CN108013219B - Application of compound microbial agent in preparation of pig feed additive - Google Patents
Application of compound microbial agent in preparation of pig feed additive Download PDFInfo
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- CN108013219B CN108013219B CN201711337692.9A CN201711337692A CN108013219B CN 108013219 B CN108013219 B CN 108013219B CN 201711337692 A CN201711337692 A CN 201711337692A CN 108013219 B CN108013219 B CN 108013219B
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 58
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 54
- 150000001875 compounds Chemical class 0.000 title claims abstract description 53
- 239000003674 animal food additive Substances 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 235000015277 pork Nutrition 0.000 claims abstract description 38
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 24
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 24
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 24
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 21
- 238000007918 intramuscular administration Methods 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims description 65
- 239000000843 powder Substances 0.000 claims description 33
- 235000018102 proteins Nutrition 0.000 claims description 32
- 102000004169 proteins and genes Human genes 0.000 claims description 32
- 108090000623 proteins and genes Proteins 0.000 claims description 32
- 229940088594 vitamin Drugs 0.000 claims description 31
- 229930003231 vitamin Natural products 0.000 claims description 31
- 235000013343 vitamin Nutrition 0.000 claims description 31
- 239000011782 vitamin Substances 0.000 claims description 31
- 108010046377 Whey Proteins Proteins 0.000 claims description 19
- 102000007544 Whey Proteins Human genes 0.000 claims description 19
- 235000021119 whey protein Nutrition 0.000 claims description 19
- 235000019733 Fish meal Nutrition 0.000 claims description 17
- -1 compound vitamin Chemical class 0.000 claims description 17
- 239000004467 fishmeal Substances 0.000 claims description 17
- 239000000463 material Substances 0.000 claims description 16
- 241000709785 Hermetia illucens Species 0.000 claims description 15
- 241000282887 Suidae Species 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 4
- 235000012054 meals Nutrition 0.000 claims 1
- 241000533950 Leucojum Species 0.000 abstract description 17
- 241000282898 Sus scrofa Species 0.000 description 36
- 150000003722 vitamin derivatives Chemical class 0.000 description 14
- 239000010699 lard oil Substances 0.000 description 12
- 239000000203 mixture Substances 0.000 description 11
- 235000013372 meat Nutrition 0.000 description 7
- 238000004321 preservation Methods 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 4
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 4
- 238000007605 air drying Methods 0.000 description 4
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 235000015278 beef Nutrition 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 2
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 description 2
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241000006384 Jeotgalibacillus marinus Species 0.000 description 2
- 102000036675 Myoglobin Human genes 0.000 description 2
- 108010062374 Myoglobin Proteins 0.000 description 2
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 2
- 235000019764 Soybean Meal Nutrition 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 description 2
- 229960002079 calcium pantothenate Drugs 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 2
- ZAKOWWREFLAJOT-UHFFFAOYSA-N d-alpha-Tocopheryl acetate Natural products CC(=O)OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-UHFFFAOYSA-N 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 229960000304 folic acid Drugs 0.000 description 2
- 235000019152 folic acid Nutrition 0.000 description 2
- 239000011724 folic acid Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 229960000367 inositol Drugs 0.000 description 2
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229960003512 nicotinic acid Drugs 0.000 description 2
- 235000001968 nicotinic acid Nutrition 0.000 description 2
- 239000011664 nicotinic acid Substances 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 229960000342 retinol acetate Drugs 0.000 description 2
- QGNJRVVDBSJHIZ-QHLGVNSISA-N retinyl acetate Chemical compound CC(=O)OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C QGNJRVVDBSJHIZ-QHLGVNSISA-N 0.000 description 2
- 235000019173 retinyl acetate Nutrition 0.000 description 2
- 239000011770 retinyl acetate Substances 0.000 description 2
- 229960002477 riboflavin Drugs 0.000 description 2
- 235000019192 riboflavin Nutrition 0.000 description 2
- 239000002151 riboflavin Substances 0.000 description 2
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000004455 soybean meal Substances 0.000 description 2
- 229940042585 tocopherol acetate Drugs 0.000 description 2
- 239000011691 vitamin B1 Substances 0.000 description 2
- 239000011715 vitamin B12 Substances 0.000 description 2
- 239000011726 vitamin B6 Substances 0.000 description 2
- 239000011647 vitamin D3 Substances 0.000 description 2
- QYSXJUFSXHHAJI-YRZJJWOYSA-N vitamin D3 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-YRZJJWOYSA-N 0.000 description 2
- 239000011652 vitamin K3 Substances 0.000 description 2
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000010775 animal oil Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000004579 marble Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000001087 myotubule Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000002807 slow-twitch muscle fiber Anatomy 0.000 description 1
- 239000003760 tallow Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Marine Sciences & Fisheries (AREA)
- Biochemistry (AREA)
- Birds (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides an application of a compound microbial agent in preparation of a pig feed additive, wherein the compound microbial agent comprises marine bacillus N6-2 and lactobacillus plantarum CMRC 10L. The invention discloses the application of the compound microbial agent as a pig feed additive for the first time, and the compound microbial agent can be applied to pig feed to play a role in improving the intramuscular fat content of pork, so that snowflake pork is obtained, and the obtained pork has higher market value.
Description
Technical Field
The invention relates to the technical field of feed additives, and in particular relates to application of a compound microbial agent in preparation of a pig feed additive.
Background
The snowflake meat is a noun for showing the meat quality, because fat is deposited among muscle fibers, the snowflake meat forms obvious marble patterns with red muscle fibers, is exactly like the snowflake and is famous, the meat quality of the snowflake meat is not fat, not greasy and not firewood when being eaten, is healthy and delicious, and therefore the snowflake meat is popular with consumers and is regarded as a treasure of the meat quality. Many people know that beef has snowflake beef, but few people know that pork also has snowflake pork. The reason is that under normal conditions, obvious snowflakes are formed only when the content of intramuscular fat in pork is more than 8%, obvious fog-like snowflakes are formed only when the content of intramuscular fat in the pork reaches more than 10%, and generally, the content of intramuscular fat in the pork is between 2 and 6%, and at the moment, marbling is not obvious, and obvious snowflake-like snowflakes are not formed. So the snowflake pork is difficult to obtain and is rarely known. It is common for the skilled person to add additives to the feed to regulate the growth and development of pigs. However, no feed additive capable of improving the pork quality to obtain snowflake pork exists in the prior art.
Disclosure of Invention
The invention aims to overcome the defects of the existing feed additive and provide a compound microbial agent, and the compound microbial agent is applied to a pig feed additive to improve the quality of pork and play a role in increasing the intramuscular fat content of the pork, so that snowflake pork is obtained, and the obtained pork has higher market value.
Another object of the present invention is to provide a pig feed additive comprising the complex microbial inoculant.
Still another object of the present invention is to provide a pig feed comprising the complex microbial inoculant.
It is yet another object of the present invention to provide a method for improving the quality of pork.
In order to achieve the purpose of the invention, the invention adopts the technical scheme that:
through animal feeding experiments, the inventor finds that the compound microbial agent comprising the bacillus marinus N6-2 and the lactobacillus plantarum CMRC10L can improve the pork quality, promote fat accumulation in muscles, play a role in increasing the intramuscular fat content in the pork and obtain the snowflake pork by applying the compound microbial agent to the pig feed additive.
Therefore, the application of the compound microbial agent in the preparation of the pig feed additive is within the protection scope of the invention, and the compound microbial agent comprises bacillus marinus N6-2 and lactobacillus plantarum CMRC 10L.
Wherein the marine Bacillus N6-2 is the existing known strain, is classified and named as Bacillus sp.N6-2, is sourced from China center for type culture Collection, has the preservation number of CCTCC NO: M2014586, and has the preservation address of Wuhan university at Lojia mountain of Wuchang city, China. The lactobacillus plantarum CMRC10L is also a known strain, is derived from the common microorganism center of China Committee for culture Collection of microorganisms, has the preservation number of CGMCC No.11341, and has the preservation address of No. 3, the institute of microbiology, China academy of sciences, North Cheng West Lu No.1, of the Korean-Yang district in Beijing.
Preferably, the compound microbial agent consists of 80-60% of marine bacillus N6-2 and 20-40% of lactobacillus plantarum CMRC10L in percentage by mass. More preferably, the compound microbial agent consists of 70 percent of marine bacillus N6-2 and 30 percent of lactobacillus plantarum CMRC10L in percentage by mass.
The invention is applicable to the general pig species, preferably, but not limited to, the species swine is Hainan.
Preferably, the pig feed additive is a feed additive capable of improving the quality of pork. Preferably, the pork is pork ham.
Specifically, the pork quality is improved by enabling the intramuscular fat content of the pork to be more than or equal to 8%. In addition, more specifically, the improvement of the pork quality also refers to the increase of the protein content of the pork. Preferably, the myoglobin in the pork is increased, the higher the concentration of the myoglobin is, the redder the pork is, and the snowflake pork can be highlighted by commonly called amber red.
In addition, a pig feed additive and a pig feed containing the compound microbial agent are also within the protection scope of the invention.
The pig feed is prepared from the following raw materials in percentage by mass: 1-8% of a compound microbial agent, 80-90% of a protein source raw material, 5-16% of a fat source raw material and 2-5% of compound vitamin.
The preparation method of the pig feed can refer to the following steps:
s1, preparing a culture medium, carrying out amplification culture on the marine bacillus N6-2 and the lactobacillus plantarum CMRC10L in the compound microbial agent, and then carrying out solid fermentation, air drying, crushing and mixing to obtain the compound microbial agent;
s2, mixing the compound microbial agent with the protein source raw material, the fat source raw material and the compound vitamin according to the proportion, and sieving after air drying to obtain the pig feed.
Preferably, the pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microbial agent, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Preferably, the protein source raw material consists of fish meal, black soldier fly larva powder and whey protein powder.
Preferably, the fish meal: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 1-2: 1 to 5.
More preferably, the fish meal: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4.
the vitamin complex generally comprises the following components in percentage by weight: vitamin B10.025wt%, riboflavin 0.1wt%, vitamin B60.075wt% of hydrochloride, 0.25wt% of nicotinic acid, 39.9wt% of inositol, 0.5wt% of calcium pantothenate, 0.25wt% of biotin, 0.025wt% of folic acid, and vitamin K30.5wt%, vitamin B12 0.25wt%, vitamin A acetate 0.25wt%, vitamin D3 0.625wt%, vitamin E acetate 7.25wt%, and medium cellulose 50 wt%.
The fat source material is animal oil such as beef tallow and lard, preferably lard.
In addition, the invention also provides a method for improving the quality of pork, and the pig feed provided by the invention is used for feeding pigs. Specifically, the method comprises the following steps: the pig feed is adopted to feed pigs of four weeks to five weeks for at least 180 days.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a new application of the compound microbial agent. The invention discloses the application of the microorganism as a pig feed additive for the first time, and the application of the microorganism in pig feed can improve the intramuscular fat content of pork, so that snowflake pork is obtained, and the obtained pork has higher market value.
Detailed Description
The present invention will be further described with reference to the following embodiments.
The present invention is further illustrated in detail by the following specific examples, it being understood that the specific examples described herein are for the purpose of illustration only and are not intended to limit the invention, and that various changes may be made within the scope of the invention as defined by the claims. The test methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials.
The marine bacillus N6-2 and the lactobacillus plantarum CMRC10L used in the implementation are all the existing known strains. Wherein the marine bacillus N6-2 is from China center for type culture Collection, the preservation number is CCTCC NO: M2014586, and the preservation address is Wuhan university at Wuchang Lojia mountain in Wuhan City, China. The lactobacillus plantarum CMRC10L is derived from the China general microbiological culture Collection center (CGMCC), the collection number is CGMCC No.11341, the collection address is No. 3 of Xilu No.1 of Beijing university of Tokyo, Chaoyang, and the institute of microbiology of China academy of sciences.
Example 1
The pig feed is prepared from the following raw materials in percentage by mass: 1% of compound microbial agent, 86% of protein source raw material, 10% of fat source raw material and 3% of compound vitamin.
Wherein the compound microbial agent consists of 70 mass percent of marine bacillus N6-2 and 30 mass percent of lactobacillus plantarum CMRC 10L.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil.
The vitamin complex comprises the following components in percentage by weight: vitamin B10.025wt%, riboflavin 0.1wt%, vitamin B60.075wt% of hydrochloride, 0.25wt% of nicotinic acid, 39.9wt% of inositol, 0.5wt% of calcium pantothenate, 0.25wt% of biotin, 0.025wt% of folic acid, and vitamin K30.5wt%, vitamin B12 0.25wt%, vitamin A acetate 0.25wt%, vitamin D3 0.625wt%, vitamin E acetate 7.25wt%, and medium cellulose 50 wt%.
The pig feed is prepared by the following steps:
s1, preparing a culture medium, carrying out amplification culture on the marine bacillus N6-2 and the lactobacillus plantarum CMRC10L in the compound microbial agent, and then carrying out solid fermentation, air drying and crushing to obtain the compound microbial agent;
s2, mixing the compound microbial agent with the protein source raw material, the fat source raw material and the compound vitamin according to the proportion, and sieving after air drying to obtain the compound microbial agent.
Example 2
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microbial agent, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the compound microbial agent consists of 70 mass percent of marine bacillus N6-2 and 30 mass percent of lactobacillus plantarum CMRC 10L.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Example 3
The pig feed is prepared from the following raw materials in percentage by mass: 8% of compound microbial agent, 80% of protein source raw material, 8% of fat source raw material and 4% of compound vitamin.
Wherein the compound microbial agent consists of 70 mass percent of marine bacillus N6-2 and 30 mass percent of lactobacillus plantarum CMRC 10L.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Example 4
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microbial agent, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the compound microbial agent consists of 70 mass percent of marine bacillus N6-2 and 30 mass percent of lactobacillus plantarum CMRC 10L.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 1: 1. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Example 5
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microbial agent, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the compound microbial agent consists of 70 mass percent of marine bacillus N6-2 and 30 mass percent of lactobacillus plantarum CMRC 10L.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 5. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Example 6
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microbial agent, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the compound microbial agent consists of 70 mass percent of marine bacillus N6-2 and 30 mass percent of lactobacillus plantarum CMRC 10L.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 1. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Example 7
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microbial agent, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the compound microbial agent consists of 80 mass percent of marine bacillus N6-2 and 20 mass percent of lactobacillus plantarum CMRC 10L.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Example 8
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microbial agent, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the compound microbial agent consists of 60 mass percent of marine bacillus N6-2 and 40 mass percent of lactobacillus plantarum CMRC 10L.
Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Comparative example 1
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microbial agent, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the compound microbial agent consists of 70 mass percent of marine bacillus N6-2 and 30 mass percent of lactobacillus plantarum CMRC 10L. The protein source raw materials only comprise fish meal and whey protein powder, wherein the fish meal: the whey protein powder is prepared from the following components in percentage by mass: 4. The fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Comparative example 2
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microbial agent, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
Wherein the compound microbial agent consists of 70 mass percent of marine bacillus N6-2 and 30 mass percent of lactobacillus plantarum CMRC 10L. The protein source raw materials are soybean meal and whey protein powder, wherein the soybean meal: the whey protein powder is prepared from the following components in percentage by mass: 4. The fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Comparative example 3
The pig feed is prepared from the following raw materials in percentage by mass: 5% of compound microbial agent, 65% of protein source raw material, 28% of fat source raw material and 2% of compound vitamin.
Wherein the compound microbial agent consists of 70 mass percent of marine bacillus N6-2 and 30 mass percent of lactobacillus plantarum CMRC 10L. Fish meal in protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Comparative example 4
The pig feed is prepared from the following raw materials in percentage by mass: lactobacillus plantarum CMRC10L 5%, protein source raw material 83%, fat source raw material 10%, and vitamin complex 2%.
Wherein, the fish meal in the protein source raw materials: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 2: 4. the fat source material is lard oil. The composition of the vitamin complex is the same as in example 1.
The preparation method is the same as that of example 1.
Test detection
In order to research the effect of the microbial feed additive, the swine species are Hainan pigs which are selected as test objects, and the test conditions are as follows: 130 pigs at five weeks of age were selected and randomized into 13 groups of 10 pigs each. 12 of the pig feeds are prepared by the above examples and comparative examples, and the rest is a blank control example (pig feed without microbial feed additive is adopted) and is fed normally for 180 days.
Thereafter, the pork hind legs (same site) were taken, and their properties were observed and tested for nutritional components, and the results are shown in tables 1 and 2:
TABLE 1 appearance Properties
TABLE 2 test results
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (8)
1. The application of the compound microbial agent in the preparation of the pig feed additive is characterized in that the compound microbial agent consists of marine bacillus N6-2 and lactobacillus plantarum CMRC 10L; the pig feed additive is a feed additive capable of improving the quality of pork; the pork quality improvement means that the intramuscular fat content of the pork is more than or equal to 8 percent.
2. The use of claim 1, wherein the complex microbial agent consists of 80-60% of marine bacillus N6-2 and 20-40% of lactobacillus plantarum CMRC10L in percentage by mass.
3. A pig feed additive, which is characterized by comprising the compound microbial agent of claim 1-2.
4. The pig feed is characterized by being prepared from the following raw materials in percentage by mass: 1-8% of the compound microbial agent, 80-90% of protein source raw material, 5-16% of fat source raw material and 2-5% of compound vitamin according to claims 1-2.
5. The pig feed according to claim 4, which is prepared from the following raw materials in percentage by mass: 5% of compound microbial agent, 83% of protein source raw material, 10% of fat source raw material and 2% of compound vitamin.
6. The pig feed according to claim 4 or 5, wherein the protein source material consists of fish meal, black soldier fly larva meal, whey protein powder.
7. The pig feed according to claim 6, characterized in that the fish meal: black soldier fly larva powder: the whey protein powder is prepared from the following components in percentage by mass: 1-2: 1 to 5.
8. A method for improving the quality of pork, wherein the pig feed according to any one of claims 4 to 7 is used for feeding pigs.
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| CN102090535A (en) * | 2010-12-23 | 2011-06-15 | 菏泽和美华饲料有限公司 | Feed for improving pig growth |
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| CN106562038A (en) * | 2016-09-23 | 2017-04-19 | 云南大北农饲料科技有限公司 | Pork quality improving functional feed |
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| CN107373092A (en) * | 2017-07-21 | 2017-11-24 | 广东越群海洋生物研究开发有限公司 | A kind of preparation method of the terrapin microcapsules opening material of zero starch |
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| CN102090535A (en) * | 2010-12-23 | 2011-06-15 | 菏泽和美华饲料有限公司 | Feed for improving pig growth |
| CN106011113A (en) * | 2016-04-28 | 2016-10-12 | 中国水产科学研究院黄海水产研究所 | Beta-glucanase generated by Bacillus marinus and preparation method thereof |
| CN106562038A (en) * | 2016-09-23 | 2017-04-19 | 云南大北农饲料科技有限公司 | Pork quality improving functional feed |
| CN106615806A (en) * | 2016-12-28 | 2017-05-10 | 南京致润生物科技有限公司 | Biologically fermented feed for improving pork quality and production method and application of biologically fermented feed |
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