CN103255080B - Excellent bacillus coagulans for breeding piglets and application thereof - Google Patents
Excellent bacillus coagulans for breeding piglets and application thereof Download PDFInfo
- Publication number
- CN103255080B CN103255080B CN201310093456.2A CN201310093456A CN103255080B CN 103255080 B CN103255080 B CN 103255080B CN 201310093456 A CN201310093456 A CN 201310093456A CN 103255080 B CN103255080 B CN 103255080B
- Authority
- CN
- China
- Prior art keywords
- bacillus coagulans
- feed
- bacillus
- coagulans
- nutrient medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses an excellent bacillus coagulans for breeding piglets. The technical scheme is as follows: the bacillus coagulans HM-09 was preserved in the China General Microbiological Culture Collection Center (State Patent Office designated patent microorganism collection center) on Oct. 29th, 2012. The collection number is CGMCC No.6729.
Description
Technical field
The present invention relates to a bacillus coagulans, specifically a strain maintenance and microecosystem regulation balance, enhancing body immunologic function for suckling piglet cultivation excellent Bacillus coagulans and application.
Background technology
Along with the development of economy and improving constantly of standard of living, the basic meaning that people more and more recognize " green ".But due to the driving of economic interests, antibiotic use has started to spread unchecked, and particularly uses in a large number in feed, cause the formation of normal microflora imbalance and suppression organism immune response in animal body, serious has caused superinfection, even can cause death; Secondly, residual in livestock and poultry body and in the food such as meat, egg, milk of microbiotic, can not make its deactivation completely through heat treated, people's this based food edible for a long time, may cause chronic poisoning etc.If use probiotics in feed, will greatly reduce antibiotic consumption, solve the problem that microbiotic can't resolve, enhancing productivity, advances in the direction to green agriculture.
The present invention is directed to above-mentioned problem, develop the excellent Bacillus coagulans HM-09(Bacillus coagulans HM-09 of a kind of suckling piglet cultivation), feed after suckling piglet as fodder additives, can in suckling piglet enteron aisle antagonism animal pathogen, maintain and microecosystem regulation balance, enhancing body immunologic function, and produce amylase, VITAMIN, promote animal digesting and assimilating and trophism feed.
Summary of the invention
The object of the present invention is to provide a strain maintenance and microecosystem regulation balance, enhancing body immunologic function for suckling piglet cultivation excellent Bacillus coagulans and application.
The present invention is achieved through the following technical solutions.
One strain is used for the excellent Bacillus coagulans of suckling piglet cultivation, it is characterized in that: described Bacillus coagulans HM-09(Bacillus coagulans HM-09) on October 29th, 2012, it is great Tun road, Chaoyang District, city of BeiJing, China in address, the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number: CGMCC No.6729.
An application for Bacillus coagulans, is characterized in that: described Bacillus coagulans is improving the application in suckling piglet production performance and diarrhoea.
Bacillus coagulans HM-09(Bacillus coagulans HM-09) be separated in health pig enteron aisle, Bacillus coagulans HM-09 effectively can suppress the growth of harmful bacteria, improvement intestine microenvironment, promote intestinal growth, strengthen intestinal function, improve feed quality, promote digesting and assimilating of feed, reduce feed-weight ratio.
A kind of bacterial strain activation of Bacillus coagulans and cultivation, it is characterized in that: the Bacilluscoagulans HM-09 of freezen protective is inoculated in GPY liquid nutrient medium, at temperature 37 DEG C, cultivate 20h, Secondary Culture like this obtains described activated b acillus coagulans HM-09 bacterial classification for 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2o1.0g, K
2hPO
42.0g, MnSO
450mg, adds 1L distilled water, regulates pH to 6.8,121 DEG C of sterilizing 15min.
The using method of Bacillus coagulans in feed, is characterized in that: stir stand-by by the bacterium powder after strain fermentation, freeze-drying, pulverizing and basal diet, and the addition of Bacillus coagulans HM-09 in feed is 1 × 10
5cfu/g.
Bacillus coagulans provided by the invention has following positively effect:
Experimental result shows, in weanling pig feed, add 1 × 10
5the Bacillus coagulans of cfu/g, can significantly improve the speed of growth of weanling pig, save feed intake, improves the economic benefit that weanling pig produces.
Test-results shows, adds 1 × 10 in weanling pig feed
5the Bacillus coagulans of cfu/g, can reduce the incidence of diarrhea of weaned piglets, and then can reduce the generation of intestines source property disease, improves feed digestibility, improves pig starter feed utilization ratio.
Accompanying drawing explanation
Accompanying drawing is that diarrhea of weaned piglets improves situation schematic diagram.
Embodiment
Below in conjunction with embodiment, the invention will be further described; Following embodiment is illustrative, does not limit the scope of the invention.
One strain is used for the excellent Bacillus coagulans of suckling piglet cultivation, described Bacillus coagulans HM-09(Bacillus coagulans HM-09) on October 29th, 2012, it is great Tun road, Chaoyang District, city of BeiJing, China in address, the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, preserving number: CGMCC No.6729.
An application for Bacillus coagulans, described Bacillus coagulans is improving the application in suckling piglet production performance and diarrhoea.
Bacillus coagulans HM-09(Bacillus coagulans HM-09) be separated in health pig enteron aisle, Bacillus coagulans HM-09 effectively can suppress the growth of harmful bacteria, improvement intestine microenvironment, promote intestinal growth, strengthen intestinal function, improve feed quality, promote digesting and assimilating of feed, reduce feed-weight ratio.
A kind of bacterial strain activation of Bacillus coagulans and cultivation, the Bacillus coagulansHM-09 of freezen protective is inoculated in GPY liquid nutrient medium, at temperature 37 DEG C, cultivate 20h, Secondary Culture like this obtains described activated b acillus coagulans HM-09 bacterial classification for 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2o1.0g, K
2hPO
42.0g, MnSO
450mg, adds 1L distilled water, regulates pH to 6.8,121 DEG C of sterilizing 15min.
The using method of Bacillus coagulans in feed, stirs stand-by by the bacterium powder after strain fermentation, freeze-drying, pulverizing and basal diet, and the addition of Bacillus coagulans HM-09 in feed is 1 × 10
5cfu/g.
Experimental technique in following embodiment, if no special instructions, is ordinary method.
The application of described Bacillus coagulans in Production Performance of Weaning Pigs, concrete grammar is as follows:
Testing program: select kind to be the sodium selenite 40 that long white 28 ages in days of hybridizing with Large White wean, be divided into 2 groups at random, often group 20 piglets, 35 days test periods; Control group fed typical corn bean pulp type basal diet, test group is fed and is added the basal diet of Bacillus coagulans.Basal diet formula and trophic level are in table 1.
Table 1 basal diet formula and trophic level
Index determining: average daily ingestion amount: every day precise and record test group and control group feeding coal, weigh during off-test and record clout amount, calculating the average daily ingestion amount of every piglet; Average daily gain: the first day (beginning) of test, 8 title piglets in the 35th day (end) morning weight on an empty stomach, calculate the average daily gain of every piglet; Feed-weight ratio: calculate test group and control group feed-weight ratio according to average daily ingestion amount and average daily gain; Diarrhea rate: after on-test, every day observes piglet ight soil situation, record test group and control group suffer from diarrhoeas piglet head, finally often to organize head number of times that piglet occurs to suffer from diarrhoea divided by total raising head number of times, calculating diarrhea rate.
Experimental animal manages: test piglet adopts high bed to raise, semi-open pig house, natural ventilation, piglet free choice feeding, and nipple water dispenser is freely drunk water; Immunity and expelling parasite program are undertaken by pig farm conventional synchronous.Timely receiving tank external loss material is put back in groove, observes at any time, records and search for food and healthy state.
Experiment 1: Bacillus coagulans HM-09(Bacillus coagulans HM-09) improve the effect of Production Performance of Weaning Pigs.
Select kind to be the sodium selenite 40 that long white 28 ages in days of hybridizing with Large White wean, be divided into 2 groups at random, often group 20 piglets.35 days test periods, test adopts typical corn bean pulp type basal diet, control group fed basal diet, and test group is fed and added the basal diet of Bacillus coagulans, coagulated bacillus living number>=1 × 10 in feed
5cfu/g.Wean suckling piglet production performance improves situation in table 2.
Table 2 Production Performance of Weaning Pigs improves situation
As seen from the experiment, add Bacillus coagulans in daily ration, the weanling pig speed of growth comparatively control group significantly improves, and average daily gain amount is 69.12 grams.
Experiment 2: Bacillus coagulans HM-09(Bacillus coagulans HM-09) improve the effect of diarrhea of weaned piglets.
Select kind to be the sodium selenite 30 that long white 28 ages in days of hybridizing with Large White wean, be divided into 2 groups at random, often group 15 piglets.30 days test periods, test adopts basal diet composition and trophic level in table 1.After on-test, every day observes piglet ight soil situation, and record test group and control group suffer from diarrhoeas piglet head, finally often to organize head number of times that piglet occurs to suffer from diarrhoea divided by total raising head number of times, calculating diarrhea rate.Test grice diarrhoea rate is shown in accompanying drawing.
As seen from the experiment, in daily ration, add Bacillus coagulans HM-09(Bacillus coagulansHM-09), diarrhea of weaned piglets rate comparatively control group has obvious reduction, and diarrhea rate is lower 32.9%.
Claims (3)
1. Bacillus coagulans (Bacillus coagulans) HM-09 is preparing the application improved in the feed of suckling piglet production performance and diarrhoea, and described Bacillus coagulans is separated to obtain from health pig enteron aisle; This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number: CGMCCNo.6729, and preservation date is on October 29th, 2012.
2. apply as claimed in claim 1, wherein the method for Bacillus coagulans activation and cultivation is: be inoculated in GPY liquid nutrient medium by the Bacillus coagulans HM-09 of freezen protective, at temperature 37 DEG C, cultivate 20h, Secondary Culture like this obtains the Bacillus coagulans HM-09 bacterial classification activated for 2-3 time; Described GPY liquid nutrient medium is composed as follows: peptone 10.0g, yeast extract paste 10.0g, glucose 6.0g, MgSO
47H
2o 1.0g, K
2hPO
42.0g, MnSO
450mg, adds 1L distilled water, regulates pH to 6.8,121 DEG C of sterilizing 15min.
3. apply as claimed in claim 1, wherein Bacillus coagulans is prepared the method for feed and is: stir stand-by by the bacterium powder after strain fermentation, freeze-drying, pulverizing and basal diet, in feed, coagulated bacillus living number reaches 1 × 10
5cfu/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310093456.2A CN103255080B (en) | 2013-03-22 | 2013-03-22 | Excellent bacillus coagulans for breeding piglets and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310093456.2A CN103255080B (en) | 2013-03-22 | 2013-03-22 | Excellent bacillus coagulans for breeding piglets and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103255080A CN103255080A (en) | 2013-08-21 |
| CN103255080B true CN103255080B (en) | 2015-05-27 |
Family
ID=48959299
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310093456.2A Active CN103255080B (en) | 2013-03-22 | 2013-03-22 | Excellent bacillus coagulans for breeding piglets and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103255080B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103243043B (en) * | 2013-03-22 | 2015-09-16 | 内蒙古和美科盛生物技术有限公司 | One strain is for improving excellent Bacillus coagulans and the application thereof of the cultivation of inferior piglet |
| CN104974967B (en) * | 2015-07-22 | 2018-04-17 | 江南大学 | One bacillus pumilus and its child care piglet compound micro-ecological preparation |
| CN106138113A (en) * | 2016-08-08 | 2016-11-23 | 大连三仪动物药品有限公司 | The biological compound preparation of preventing and treating livestock and poultry diarrhea |
| CN110396480A (en) * | 2018-04-24 | 2019-11-01 | 广东怡和科洁科技有限公司 | A kind of bacillus coagulans XP and its application in Feed Manufacturing |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102505003A (en) * | 2011-12-19 | 2012-06-20 | 湖南省微生物研究所 | High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof |
-
2013
- 2013-03-22 CN CN201310093456.2A patent/CN103255080B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102505003A (en) * | 2011-12-19 | 2012-06-20 | 湖南省微生物研究所 | High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 不同芽孢杆菌对断奶仔猪生产性能的影响;周映华 等;《饲料工业》;20120210;第33卷(第3期);21-23 * |
| 凝结芽孢杆菌分离筛选及复合益生菌剂的应用研究;戴青;《中国优秀硕士学位论文全文数据库农业科技辑》;20090215(第2期);D050-52 * |
| 凝结芽孢杆菌替代抗生素对猪生产性能的影响;李国建;《河南农业科学》;20041015(第357期);72-74 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103255080A (en) | 2013-08-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102168045B (en) | Bacillus subtilis preparation and preparation method thereof | |
| CN102204632B (en) | Microbial feed additive for increasing livestock and poultry product quality and improving meat egg milk flavor and preparation method thereof | |
| CN101530161B (en) | Novel feed additive and preparation method thereof | |
| CN103039699B (en) | Mixed fermentation feed and preparation method and application method of mixed fermentation feed | |
| CN102326677B (en) | Composite micro-ecological feed additive and preparation method and premix thereof | |
| CN101463329B (en) | Saccharomyces cerevisiae, yeast preparation including the same, feed, and preparation and use thereof | |
| CN106591182B (en) | A kind of probiotics of fermentation of bacillus subtilis Radix Astragali and its application | |
| CN107173522A (en) | A kind of fermented feed containing probiotics and its preparation method and application | |
| CN101946850B (en) | Lactobacillus reuteri fermented liquid feed, preparation method and application thereof | |
| CN103966124A (en) | Compound microecological preparation and additive, as well as premix thereof | |
| CN103981118A (en) | Bacillus subtilis feed additive and preparation method and application thereof | |
| CN102742728A (en) | Compound probiotic feed additive for livestock and poultry, and premix, concentrate feed and mixed feed containing same | |
| CN103205375B (en) | Quality clostridium butyricum for breeding egg-laying poultry | |
| CN103907749A (en) | Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof | |
| CN103205373B (en) | Quality clostridium butyricum for breeding piglets | |
| CN102907563A (en) | Method for preparing high-activity probiotic preparation for livestock breeding | |
| CN103255080B (en) | Excellent bacillus coagulans for breeding piglets and application thereof | |
| CN102860411A (en) | Lactobacillus rhamnosus probiotic and preparation method thereof | |
| CN103039716A (en) | Feed additive, premix and batch | |
| CN109924360A (en) | One boar fermentation compound feed and preparation method thereof | |
| CN101933558B (en) | Compound microecological preparation and application thereof | |
| CN103255082B (en) | One plant of excellent bacillus coagulans and its application for growing and fattening pigs cultivation | |
| CN102342371A (en) | Process for preparing compound feed additive from bacillus subtilis natto and bacillus licheniformis | |
| CN103205377B (en) | Quality bacillus coagulans for breeding broilers and application of excellent bacillus coagulans | |
| CN111647541A (en) | Clostridium butyricum viable bacteria preparation, production method thereof and animal feed additive |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |