CN107937311A - The streptococcus thermophilus of one plant height production gamma aminobutyric acid, preserve cultural method and the method that acidified milk is prepared using the streptococcus thermophilus - Google Patents
The streptococcus thermophilus of one plant height production gamma aminobutyric acid, preserve cultural method and the method that acidified milk is prepared using the streptococcus thermophilus Download PDFInfo
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Abstract
The invention discloses a plant height to produce gamma aminobutyric acid streptococcus thermophilus, its preserving number is CGMCC No.14810；Disclosed herein as well is the preservation cultural method of the streptococcus thermophilus of the high yield gamma aminobutyric acid, include the following steps：(1) streptococcus thermophilus containing gamma aminobutyric acid is isolated from koumiss；(2) the production gamma aminobutyric acid streptococcus thermophilus that step (1) obtains is inoculated on the slant medium of suitable streptococcus thermophilus growth；(3) after step (2) inclined-plane culture, it is inoculated in Anaerobic culturel in fluid nutrient medium；The invention also discloses utilize the method for producing gamma aminobutyric acid streptococcus thermophilus fermentation.It is an advantage of the current invention that the strain energy high yield gamma aminobutyric acid, and the speed of growth is fast in milk, and the content with gamma aminobutyric acid in the fermented product of bacterium preparation is high, has great application value.
Description
Technical field
The invention belongs to microorganism and technical field of microbial fermentation, is specially one plant of thermophilic chain of highly producing gamma-aminobutyric acid
Coccus, the method for preserving culture and the method that acidified milk is prepared using the streptococcus thermophilus.
Background technology
γ-aminobutyric acid (γ-amino butyric acid, GABA), is a kind of natural feature nonprotein ammonia
Base acid, is widely present in the various animal and plant bodies such as tea, radish leaves, shell-fish, mammal.γ-aminobutyric acid in human body is not
Only pass on material high concentration to be present in central nervous system as inhibiting nerve, be also present in the tip internal organs such as enteron aisle.γ-
Aminobutyric acid is a kind of physiologically active ingredient, has the glucose metabolism of activation intracerebral, reduce blood ammonia, anticonvulsion, blood pressure lowering, enhancement brain
Vigor, increase neurotrophy, enhancing long-term memory, promotion growth hormone secretion, activation renal function, liver function, improve climacteric
The important physiological functions such as syndrome.γ-aminobutyric acid has good medical applications prospect；It is a kind of novel foodstuff activity again at the same time
The factor, the application in functional food become research hotspot.
In the prior art, general functional food is all it is rich in γ-aminobutyric acid by way of outer addition, and day
Right γ-aminobutyric acid is expensive, which has limited its additive amount in food, limits the extensive of γ-aminobutyric acid
Using.Existing research is attempted the strain for producing γ-aminobutyric acid being applied in food, but the production that most of scholar screens
The yield that the strain of γ-aminobutyric acid either has is too low or not in the strain applied in food or in milk
It cannot grow, similarly limit application of the strain of production γ-aminobutyric acid in acidified milk, the present situation is urgently to be resolved hurrily.
The content of the invention
For solve the domestic and international γ-aminobutyric acid enhanced products of the prior art be all by add γ-aminobutyric acid dispensing come
Realize, producing life, this is higher, and the strain of the production γ-aminobutyric acid of open report cannot be grown in milk, or in milk
Middle growth yield is also than relatively low, product that both modes produce is all there may be alpha-aminobutyric acid content in product is relatively low, no
The defects of additive amount of its Function can be reached, the present invention provides one plant of highly producing gamma-aminobutyric acid streptococcus thermophilus, preserve
The method of culture and the method that acidified milk is prepared using the streptococcus thermophilus, the purpose of realization can be in milk quickly for the strain
Grow and produce the γ-aminobutyric acid of high yield, there is great application value.
To achieve these goals, the present invention provides following technical scheme：A plant height provided by the invention produces gamma-amino
The streptococcus thermophilus of butyric acid, the streptococcus thermophilus preserving number are CGMCC No.14810, and Classification And Nomenclature is streptococcus thermophilus
Streptococcus thermophilus.The strain was preserved in Chinese microorganism strain preservation management committee before the applying date
Member can common micro-organisms center (CGMCC).Address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences microorganism
Research institute, postcode 100101, preservation date are on October 16th, 2017.
The cultural method of production γ-aminobutyric acid streptococcus thermophilus provided by the invention, it includes the following steps：
(1) streptococcus thermophilus for producing γ-aminobutyric acid is separated from koumiss；
(2) the production γ-aminobutyric acid streptococcus thermophilus that step (1) obtains is inoculated in the oblique of suitable streptococcus thermophilus growth
On the culture medium of face；
(3) after step (2) inclined-plane culture, it is inoculated in Anaerobic culturel in fluid nutrient medium.
Glutamic acid or sodium glutamate can be converted into gamma-amino by this streptococcus thermophilus that the present invention obtains in milk
Butyric acid, obtained acidified milk containing γ-aminobutyric acid, which can directly be eaten, either configures beverage or other products, to play its guarantor
Strong effect, the cost of extraction and processing after being needed when reducing traditional mode of production γ-aminobutyric acid dispensing.
Preferably, the slant medium that streptococcus thermophilus growth is adapted in the step (2) is M17 culture mediums.
Further, the PH of inclined-plane culture is 6.5-7.5 in the step (2), and temperature is 37-45 DEG C, and incubation time is
24-72h。
Further, fluid nutrient medium is skim milk in the step (3).
Further, the temperature cultivated in the step (3) in liquid medium within is 37-42 DEG C, incubation time 16-
24h。
The production γ-aminobutyric acid streptococcus thermophilus obtained by above-mentioned cultural method, preserving number is also claimed in the present invention
It is CGMCC No.14810.
The invention also discloses the method for preparing the acidified milk containing above-mentioned production γ-aminobutyric acid streptococcus thermophilus, including such as
Lower step：(1) slant activation culture：Production γ-aminobutyric acid streptococcus thermophilus is activated using M17 slant mediums；
(2) liquid seeds culture：The streptococcus thermophilus of highly producing gamma-aminobutyric acid after step (1) is activated is added to liquid
Anaerobic culturel is carried out in body culture medium；
(3) inoculation fermentation：By by step (2) culture liquid seeds and treat fermented milk products according to volume ratio 5%~
10% be inoculated with after anaerobic fermentation obtain rich in γ-aminobutyric acid acidified milk.
Further, the PH of M17 slant mediums is 6.5-7.5 in the step (1), and activation temperature is 37-45 DEG C, living
The change time is 24-72h.
Further, fluid nutrient medium is skim milk in the step (2), and the temperature cultivated in fluid nutrient medium is
37-42 DEG C, incubation time 16-24h.
Further, treat that fermented milk products are added with protein hydrolysate in step (3), its additive amount is 1-10g/kg.
Further, protein hydrolysate includes soy bean protein hydrolysate, milk protein hydrolysate, pea protein hydrolysate, yeast hydrolysis
Any one in albumen or more than one.
Further, treat that fermented milk products are added with sodium glutamate, its additive amount 1-20g/Kg in step (3).
Further, above-described fermentation temperature is 37-42 DEG C, fermentation time 24-72h.
The present invention uses above-mentioned technical proposal, including following beneficial effect：Can be with volume production γ-ammonia by the strain of the present invention
Base butyric acid, γ-aminobutyric acid yield can reach 1-7g/kg products, and can be by controlling sodium glutamate and protolysate
Amount, be enriched with required γ-aminobutyric acid yield.Traditional γ-aminobutyric acid dispensing is by fermentation, is then isolated and purified
Obtain, product cost is high.Traditional is only that with the addition of this function dispensing rich in γ-aminobutyric acid product, so when addition
Cost is also higher when measuring high, the present invention directly by fermentation method obtain the dairy products containing γ-aminobutyric acid, saved purchase γ-
The cost of aminobutyric acid dispensing, has great commercial value and the market competitiveness.γ-aminobutyric acid dispensing has multiple functions
Property can meet the different health demands of consumer, have great social effect.
Brief description of the drawings
Fig. 1 is phylogenetic analysis figure collection of illustrative plates of the streptococcus thermophilus of the present invention based on genome
Accession number CP025399 of the complete genome sequence of the bacterium on NCBI.
16 plants of the streptococcus thermophilus of full genome sequencing, its evolution based on full-length genome with this bacterial strain are chosen on ncbi
Tree analysis is as shown in Figure 1.
Embodiment
The present invention is described in further detail below by specific embodiment.
Embodiment one：A kind of cultural method for producing γ-aminobutyric acid streptococcus thermophilus provided by the invention, including following step
Suddenly：
(1) streptococcus thermophilus containing γ-aminobutyric acid is isolated from koumiss；
(2) the production γ-aminobutyric acid streptococcus thermophilus that step (1) obtains is inoculated in the oblique of suitable streptococcus thermophilus growth
On the culture medium of face；
(3) after step (2) inclined-plane culture, it is inoculated in Anaerobic culturel in fluid nutrient medium.
The slant medium for being adapted to streptococcus thermophilus growth in the step (2) is M17 culture mediums, in the step (2)
The PH of inclined-plane culture is 6.5, and temperature is 37 DEG C, incubation time 24h.Fluid nutrient medium is skim milk in the step (3).
The temperature cultivated in the step (3) in liquid medium within is 37 DEG C, incubation time 24h.
The invention also discloses using the method for producing γ-aminobutyric acid streptococcus thermophilus fermentation, include the following steps：(1)
Slant activation culture：The streptococcus thermophilus of highly producing gamma-aminobutyric acid is activated using M17 slant mediums；
(2) liquid seeds culture：The streptococcus thermophilus of highly producing gamma-aminobutyric acid after step (1) is activated is added to liquid
Anaerobic culturel is carried out in body culture medium；
(3) inoculation fermentation：By the liquid seeds by step (2) culture with treating that fermented milk products are carried out according to volume ratio 5%
Anaerobic fermentation obtains the acidified milk rich in γ-aminobutyric acid after inoculation.
The PH of M17 slant mediums is 6.5 in the step (1), and activation temperature is 37 DEG C, soak time 24h.It is described
Fluid nutrient medium is skim milk in step (2), and the temperature cultivated in fluid nutrient medium is 37 DEG C, incubation time 24h.It is described
The protein hydrolysate of addition includes soy bean protein hydrolysate, milk protein hydrolysate, additive amount 1g/kg, the additive amount in step (3)
Refer to mass concentration of the protein hydrolysate in tunning.Fluid nutrient medium and milk in the step (3) after Anaerobic culturel
Volume ratio be 10:100, it can also add raw material common in the prior art acidified milk such as white granulated sugar, glutamic acid, the temperature of fermentation
Spend for 42 DEG C, fermentation time 24h.The acidified milk rich in γ-aminobutyric acid of γ-aminobutyric acid 1.2g/Kg must be contained by obtaining.
Embodiment two：A kind of cultural method for producing γ-aminobutyric acid streptococcus thermophilus provided by the invention, including following step
Suddenly：
(1) streptococcus thermophilus containing γ-aminobutyric acid is isolated from koumiss；
(2) the production γ-aminobutyric acid streptococcus thermophilus that step (1) obtains is inoculated in the oblique of suitable streptococcus thermophilus growth
On the culture medium of face；
(3) after step (2) inclined-plane culture, it is inoculated in Anaerobic culturel in fluid nutrient medium.
The slant medium for being adapted to streptococcus thermophilus growth in the step (2) is M17 culture mediums, in the step (2)
The PH of inclined-plane culture is 7.5, and temperature is 45 DEG C, incubation time 72h.Fluid nutrient medium is skim milk in the step (3).
The temperature cultivated in the step (3) in liquid medium within is 42 DEG C, incubation time 16h.
Production γ-aminobutyric acid streptococcus thermophilus through above method culture, preserving number are CGMCC No.14810.
The invention also discloses the method for preparing acidified milk using γ-aminobutyric acid streptococcus thermophilus is produced, including following step
Suddenly：(1) slant activation culture：The streptococcus thermophilus of highly producing gamma-aminobutyric acid is activated using M17 slant mediums；
(2) liquid seeds culture：The streptococcus thermophilus of highly producing gamma-aminobutyric acid after step (1) is activated is added to liquid
Anaerobic culturel is carried out in body culture medium；
(3) inoculation fermentation：By the liquid seeds by step (2) culture with treating that fermented milk products are carried out according to volume ratio 10%
Anaerobic fermentation obtains the acidified milk rich in γ-aminobutyric acid after inoculation.
The PH of M17 slant mediums is 7.5 in the step (1), and activation temperature is 45 DEG C, soak time 72h.It is described
Fluid nutrient medium is skim milk in step (2), and the temperature cultivated in fluid nutrient medium is 42 DEG C, incubation time 16h.It is described
The protein hydrolysate of addition includes pea protein hydrolysate, yeast protein hydrolysate, additive amount 10g/kg, the addition in step (3)
Amount refers to mass concentration of the protein hydrolysate in tunning.Fluid nutrient medium after Anaerobic culturel and ox in the step (3)
The volume ratio of milk is 5:100, raw material common in the prior art acidified milk such as white granulated sugar, glutamic acid can be also added, fermentation
Temperature is 37 DEG C, fermentation time 72h.Obtain the acidified milk of the 6g/Kg containing γ-aminobutyric acid.
Embodiment three：A kind of cultural method for producing γ-aminobutyric acid streptococcus thermophilus provided by the invention, including following step
Suddenly：
(1) streptococcus thermophilus for producing γ-aminobutyric acid is separated from koumiss；
(2) the production γ-aminobutyric acid streptococcus thermophilus that step (1) obtains is inoculated in the oblique of suitable streptococcus thermophilus growth
On the culture medium of face；
(3) after step (2) inclined-plane culture, it is inoculated in Anaerobic culturel in fluid nutrient medium.
The slant medium for being adapted to streptococcus thermophilus growth in the step (2) is M17 culture mediums, in the step (2)
The PH of inclined-plane culture is 7, and temperature is 42 DEG C, incubation time 48h.Fluid nutrient medium is skim milk in the step (3).Institute
It is 39 DEG C to state the temperature cultivated in step (3) in liquid medium within, incubation time 20h.
γ-aminobutyric acid streptococcus thermophilus through above method culture, preserving number are CGMCC No.14810.
The invention also discloses the method for preparing acidified milk using γ-aminobutyric acid streptococcus thermophilus is produced, including following step
Suddenly：(1) slant activation culture：The streptococcus thermophilus of highly producing gamma-aminobutyric acid is activated using M17 slant mediums；
(2) liquid seeds culture：The streptococcus thermophilus of highly producing gamma-aminobutyric acid after step (1) is activated is added to liquid
Anaerobic culturel is carried out in body culture medium；
(3) inoculation fermentation：By the liquid seeds by step (2) culture with treating that fermented milk products are carried out according to volume ratio 7%
Anaerobic fermentation obtains the acidified milk rich in γ-aminobutyric acid after inoculation.
The PH of M17 slant mediums is 7 in the step (1), and activation temperature is 42 DEG C, soak time 48h.The step
Suddenly fluid nutrient medium is skim milk in (2), and the temperature cultivated in fluid nutrient medium is 38 DEG C, incubation time 20h.The step
Suddenly the protein hydrolysate of addition includes pea protein hydrolysate, yeast protein hydrolysate, additive amount 5g/kg in (3), and the additive amount is
Refer to mass concentration of the protein hydrolysate in tunning.Fluid nutrient medium and milk in the step (3) after Anaerobic culturel
Volume ratio is 8:100, it can also add raw material common in the prior art acidified milk such as white granulated sugar, glutamic acid, the temperature of fermentation
For 39 DEG C, fermentation time 48h.Obtain the acidified milk rich in γ-aminobutyric acid of the 7g/Kg containing γ-aminobutyric acid.
The production γ-aminobutyric acid strains of streptococcus thermophilus that the present invention obtains fermentation 72h yield in the case where only adding MSG
5g/kg can be reached, yield can reach 5g/kg in 48h in the case where adding protolysate, shorten fermentation time
Improve production efficiency.
The foregoing is only a preferred embodiment of the present invention, is not intended to limit the invention, for the skill of this area
For art personnel, the invention may be variously modified and varied.Within the spirit and principles of the invention, that is made any repaiies
Change, equivalent substitution, improvement etc., should all be included in the protection scope of the present invention.
Claims (13)
1. the streptococcus thermophilus of one plant of highly producing gamma-aminobutyric acid, it is characterised in that the streptococcus thermophilus preserving number is CGMCC
No.14810。
2. the preservation cultural method of the streptococcus thermophilus of the highly producing gamma-aminobutyric acid described in claim 1, it is characterised in that should
Method includes the following steps：
(1) streptococcus thermophilus of highly producing gamma-aminobutyric acid is isolated from koumiss, its preserving number is CGMCC No.14810；
(2) streptococcus thermophilus for the highly producing gamma-aminobutyric acid for obtaining step (1) is inoculated in the oblique of suitable streptococcus thermophilus growth
On the culture medium of face；
(3) streptococcus thermophilus is inoculated in Anaerobic culturel in fluid nutrient medium after step (2) inclined-plane culture.
3. the preservation cultural method of the streptococcus thermophilus of highly producing gamma-aminobutyric acid according to claim 2, its feature exist
In the slant medium for being adapted to streptococcus thermophilus growth in step (2) is M17 culture mediums.
4. the preservation cultural method of the streptococcus thermophilus of highly producing gamma-aminobutyric acid according to claim 2, its feature exist
In the PH of slant medium is 6.5-7.5 in step (2), and temperature is 37-45 DEG C, incubation time 24-72h.
5. the preservation cultural method of the streptococcus thermophilus of highly producing gamma-aminobutyric acid according to claim 2, its feature exist
In fluid nutrient medium is skim milk in step (3).
6. the preservation cultural method of the streptococcus thermophilus of highly producing gamma-aminobutyric acid according to claim 2, its feature exist
In the temperature cultivated in step (3) in liquid medium within is 37-42 DEG C, incubation time 16-24h.
7. the method for acidified milk is prepared using the streptococcus thermophilus of highly producing gamma-aminobutyric acid, it is characterised in that this method includes step
It is rapid as follows：
(1) slant activation culture：The streptococcus thermophilus of highly producing gamma-aminobutyric acid is activated using M17 slant mediums；
(2) liquid seeds culture：The streptococcus thermophilus of highly producing gamma-aminobutyric acid after step (1) is activated is added to liquid training
Support in base and carry out Anaerobic culturel；
(3) inoculation fermentation：By by step (2) culture liquid seeds and treat fermented milk products according to volume ratio 5%~10% into
Anaerobic fermentation obtains the acidified milk rich in γ-aminobutyric acid after row inoculation.
8. the method that the streptococcus thermophilus according to claim 7 using highly producing gamma-aminobutyric acid prepares acidified milk, it is special
Sign is that the pH of M17 slant mediums is 6.5-7.5 in step (1), and activation temperature is 37-45 DEG C, soak time 24-
72h。
9. the method that the streptococcus thermophilus according to claim 7 using highly producing gamma-aminobutyric acid prepares acidified milk, it is special
Sign is that fluid nutrient medium is skim milk in step (2), and the temperature cultivated in fluid nutrient medium is 37-42 DEG C, incubation time
For 16-24h.
10. the method that the streptococcus thermophilus according to claim 7 using highly producing gamma-aminobutyric acid prepares acidified milk, its
It is characterized in that, treats that fermented milk products are added with protein hydrolysate in step (3), its additive amount is 1-10g/kg.
11. the method that the streptococcus thermophilus according to claim 10 using highly producing gamma-aminobutyric acid prepares acidified milk, its
It is characterized in that, protein hydrolysate includes appointing in soy bean protein hydrolysate, milk protein hydrolysate, pea protein hydrolysate, yeast protein hydrolysate
Meaning is one or more kinds of.
12. the method that the streptococcus thermophilus according to claim 7 using highly producing gamma-aminobutyric acid prepares acidified milk, its
It is characterized in that, treats that fermented milk products are added with sodium glutamate, its additive amount 1-20g/Kg in step (3).
13. acidified milk is prepared using the streptococcus thermophilus of highly producing gamma-aminobutyric acid according to claim 7,10,11 or 12
Method, it is characterised in that the temperature of fermentation be 37-42 DEG C, fermentation time 24-72h.
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| CN110283855A (en) * | 2019-06-20 | 2019-09-27 | 绿雪生物工程(深圳)有限公司 | A method of it improving 210202 bacterial strain of streptococcus thermophilus and produces GABA ability |
| CN112616936A (en) * | 2020-12-30 | 2021-04-09 | 光明乳业股份有限公司 | Milk powder for assisting sleep and preparation method thereof |
| CN113272418A (en) * | 2021-03-17 | 2021-08-17 | 微康益生菌(苏州)股份有限公司 | Streptococcus thermophilus for producing gamma-aminobutyric acid and application thereof |
| CN114437971A (en) * | 2022-01-24 | 2022-05-06 | 浙江一鸣食品股份有限公司 | Streptococcus thermophilus capable of highly producing gamma-aminobutyric acid and efficiently converting sodium glutamate as well as culture method and application of streptococcus thermophilus |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1710088A (en) * | 2005-06-24 | 2005-12-21 | 南京农业大学 | Method for producing gamma-propalanine using saliva chain coccus thermophilous subspecies |
| WO2007097374A1 (en) * | 2006-02-21 | 2007-08-30 | Kikkoman Corporation | Lactic acid bacterium capable of producing ϝ-aminobutyric acid |
| KR20080100068A (en) * | 2007-05-11 | 2008-11-14 | 우석대학교 산학협력단 | Lactobacillus buchneri opy-2 and fermented milk containing thereof |
| CN104293699A (en) * | 2014-09-17 | 2015-01-21 | 山东大学 | Streptococcus thermophilus with synthetic capability of gamma-aminobutyric acid and glutathione and application of streptococcus thermophilus |
-
2017
- 2017-12-20 CN CN201711386926.9A patent/CN107937311B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1710088A (en) * | 2005-06-24 | 2005-12-21 | 南京农业大学 | Method for producing gamma-propalanine using saliva chain coccus thermophilous subspecies |
| WO2007097374A1 (en) * | 2006-02-21 | 2007-08-30 | Kikkoman Corporation | Lactic acid bacterium capable of producing ϝ-aminobutyric acid |
| KR20080100068A (en) * | 2007-05-11 | 2008-11-14 | 우석대학교 산학협력단 | Lactobacillus buchneri opy-2 and fermented milk containing thereof |
| CN104293699A (en) * | 2014-09-17 | 2015-01-21 | 山东大学 | Streptococcus thermophilus with synthetic capability of gamma-aminobutyric acid and glutathione and application of streptococcus thermophilus |
Non-Patent Citations (3)
| Title |
|---|
| LIN CHEN等: "γ-Aminobutyric acid-rich yogurt fermented by Streptococcus salivarius subsp. thermophiles fmb5 apprars to have anti-diabetic effect on streptozotocin-induced diabetic mice", 《JOURNAL OF FUNCTIONAL FOODS》 * |
| 杨胜远等: "一株产谷氨酸脱羧酶乳酸菌的鉴定及其酶学性质", 《工业微生物》 * |
| 许本发等编著: "《酸奶和乳酸菌饮料加工》", 31 January 1994, 中国轻工业出版社 * |
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|---|---|---|---|---|
| CN109486727A (en) * | 2018-12-28 | 2019-03-19 | 光明乳业股份有限公司 | A kind of dairy starter and preparation method thereof rich in γ-aminobutyric acid |
| CN110283855A (en) * | 2019-06-20 | 2019-09-27 | 绿雪生物工程(深圳)有限公司 | A method of it improving 210202 bacterial strain of streptococcus thermophilus and produces GABA ability |
| CN112616936A (en) * | 2020-12-30 | 2021-04-09 | 光明乳业股份有限公司 | Milk powder for assisting sleep and preparation method thereof |
| CN113272418A (en) * | 2021-03-17 | 2021-08-17 | 微康益生菌(苏州)股份有限公司 | Streptococcus thermophilus for producing gamma-aminobutyric acid and application thereof |
| WO2022193163A1 (en) * | 2021-03-17 | 2022-09-22 | 微康益生菌(苏州)股份有限公司 | STREPTOCOCCUS THERMOPHILUS PRODUCING γ-AMINOBUTYRIC ACID AND APPLICATION THEREOF |
| CN114437971A (en) * | 2022-01-24 | 2022-05-06 | 浙江一鸣食品股份有限公司 | Streptococcus thermophilus capable of highly producing gamma-aminobutyric acid and efficiently converting sodium glutamate as well as culture method and application of streptococcus thermophilus |
| CN114437971B (en) * | 2022-01-24 | 2023-08-08 | 浙江一鸣食品股份有限公司 | Streptococcus thermophilus capable of producing gamma-aminobutyric acid in high yield and converting sodium glutamate in high efficiency, and culture method and application thereof |
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