CN104783167B - Sugar-free meal enzyme liquid of compound generation and preparation technology thereof - Google Patents

Sugar-free meal enzyme liquid of compound generation and preparation technology thereof Download PDF

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CN104783167B
CN104783167B CN201510216016.0A CN201510216016A CN104783167B CN 104783167 B CN104783167 B CN 104783167B CN 201510216016 A CN201510216016 A CN 201510216016A CN 104783167 B CN104783167 B CN 104783167B
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powder
enzyme liquid
meal
parts
generation
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CN104783167A (en
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祁奕心
钟瑞源
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Abstract

A kind of compound generation meal enzyme liquid in weight losing meal-replacing field and preparation method thereof, ferment stoste and medicinal material additive by obtaining after one time fermentation carry out secondary fermentation, carry out three fermentations after being mixed with generation meal agent, candida utili bacterium and glycerine by the optimization enzyme liquid obtained again, prepare compound generation meal enzyme liquid.The meal enzyme liquid of compound generation that the present invention prepares can reach the replacement diet function that half fasting has no side effect the short time, and nutrition delicious food, strengthening spleen and nourishing stomach, regulation endocrine, activate immunoblastic lymphoma system, benefiting qi and nourishing blood, toxin expelling go oedema lose weight, have generation eat effect.

Description

Sugar-free meal enzyme liquid of compound generation and preparation technology thereof
Technical field
The present invention relates to the technology in a kind of sugar-free weight-losing meal-substituting foodstuff field, specifically a kind of sugar-free meal ferment of compound generation Element liquid and preparation technology thereof.
Background technology
Scientific research finds, from unicellular lower eukaryote fungi, the mouse of fruit bat to mammal and dog class, limits Energy intaking equal Can play the effect extending bio-longevity, i.e. the animal of " hungry " not only lives longer, and lives more healthy.Molecular biology Research display, mTOR closely-related with aging (mammal Rapamycin acceptor) signal transduction pathway is in the thorn of food Swash lower activation degree the highest.Zooperal result of study naturally enough can be generalized to mankind itself.From prevention from suffering from the diseases For angle, go on a diet and be conducive to long life.
Publish on Cell Stem Cell magazine under Cell: scientists finds, the most long-time fasting Immune system not only being damaged (major side effects of chemotherapy) protected effect, and can also regenerate by inducing immune system, order is stopped The stem cell slept starts to update.This is that people find first, and natural interference means can activate stem cell, promotes organ or system Regeneration.
As can be seen here, correct is jejunitas not only harmless to function of human body, and can also improve some function of human body, Defatting beauty, disease-relieving and physique enhancing, aspect of promoting longevity have positive role.
Ferment is a kind of active macromolecules with biocatalysis, the condition that ferment can be the gentleest in body Under, the various biochemical reactions of efficient catalysis, enhance metabolism and adjust endocrine, elimination causes because of endocrinopathy Obesity, decomposes and gets rid of enteron aisle and internal poisonous substance, readjusts balanced ability of human body, promotes that the fat of deposition is a large amount of and decomposes.Ferment What jejunitas method of weight-reducing was used is that heat enters to consume many science fat-reducing principles less.But owing to the acidity of conventional ferment is high, the most again Meeting gastric acid secretion, increase appetite, satiety extreme difference, feel unbearably hungry when the jejunitas fat-reducing of user's ferment is interim, and at knot The problem such as gastric ulcer, duodenal ulcer is often induced after bundle.
At present, the most popular generation meal class diet products are mainly nutrient class and fruit-vegetable fibre class, and product is mainly adopted By the formulation of solid beverage, although a lot of meal replacement powder can ensure that balanced in nutrition, instant, but most product is full There is clearly disadvantageous part in abdomen sense, a lot of products can only replace part to eat, and the mouthfeel of product and formulation are dull, allow The crowd of decontrol body weight, when selecting meal replacing food, easily loses edible interest to the generation meal product of this dullness, is difficult to adhere to Slimming Program.And owing to generation meal class diet products lack beneficial flora and biology enzyme system i.e. ferment, when user terminates generation meal After weight loss program, gut flora can be caused to degenerate, human consumption's function reduces, the taste qi and blood cold of insufficiency type, thus induces weight gain Phenomenon.
In sum, existing enzyme liquid and meal replacing food patented product, all the most each on nutrition and health care with full abdomen Having shortcoming, the shortcoming of enzyme liquid is the losses of unavoidable intestines and stomach, is then to cause digestive function to fail for food product, does not gathers around There is the health care effect of ferment.
Therefore, when using diet products, it is more desirable to while weight-reducing be possible not only to that there is protection intestines and stomach surface Form protective layer, reduce gastric acid secretion time delay gastric emptying, adjust gastrointestinal bacterial flora, improve satiety;Pancreas, spleen can also be activated Dirty, liver, accelerates metabolism and blood circulation, decomposes and burn fat, purged body endotoxin, dredge cardiovascular drift.
Summary of the invention
The present invention is directed to deficiencies of the prior art, propose one meal enzyme liquid of compound generation and preparation technology thereof, The meal enzyme liquid of compound generation prepared can reach the replacement diet function that half fasting has no side effect the short time, and nutrition is beautiful Taste, strengthening spleen and nourishing stomach, regulation endocrine, activate immunoblastic lymphoma system, benefiting qi and nourishing blood, toxin expelling go oedema lose weight, have generation meal imitate Really.
The present invention is achieved by the following technical solutions:
The present invention relates to the preparation method of meal enzyme liquid of a kind of compound generation, by the ferment stoste that will obtain after one time fermentation Secondary fermentation is carried out with medicinal material additive, then by the optimization enzyme liquid obtained and generation meal agent, candida utili bacterium and glycerine Carry out three fermentations after mixing, prepare compound generation meal enzyme liquid.
Described one time fermentation refers to: part by weight is followed successively by 100 parts of water, 1 part of trichoderma reesei powder, 2 parts of plant breast bars Bacterium powder, 15 parts of glycerine, 5 parts of nicotinic acid, 10 parts of calcium monohydrogen phosphates and 10 parts of cholate mixing seal, and then carrying out temperature is 38.5 DEG C~40 DEG C, the time is the anaerobic fermentation of 7~14 days, obtains ferment stoste.
Described secondary fermentation refers to: adds in ferment stoste and includes duck wheat powder of straw, green tea powder, scouring rush's powder, turn over White grass meal, sweet wormwood powder, cortex acanthopanacis powder or the medicinal material additive of selfheal powder or a combination thereof, then carrying out temperature is 38.5 DEG C~40 DEG C, the time is 15~30 days anaerobic fermentations, takes supernatant and i.e. optimizes enzyme liquid.
The described preferred consumption of medicinal material additive and part by weight be: duck wheat powder of straw 1~6 parts, green tea powder 1~5 Part, scouring rush's powder 1~2 parts, potentilla discolor powder 1~5 parts, sweet wormwood powder 1~4 parts, cortex acanthopanacis powder 1~5 parts, selfheal powder 1~3 parts with 100 parts of ferment stostes, further preferred mass ratio is 6:5:2:5:4:5:3:100.
Three times described fermentations refer to: optimization enzyme liquid carries out ultrahigh-pressure sterilization inactivation, then with candida utili Bacterium, water, glycerine and generation meal agent mixs and seal that then to carry out temperature be 25 DEG C~28 DEG C, and three times of time 4~7 days are fermented, complete After one-tenth, bacteria inactivation i.e. obtains compound generation meal enzyme liquid.
Described ultrahigh-pressure sterilization inactivation refers to: be sterilized 20 minutes and 3 times repeatedly under P=600MPa environment.
The consumption preferred mass ratio of described supernatant, candida utili bacterium, water, glycerine and generation meal agent is: 50: 10:50:15:2.
The purity of above-mentioned glycerine, i.e. mass concentration ratio are 85%.
Described generation meal agent includes: pectin, Arabic gum, xanthans and guar gum, preferably its mass ratio be: pectin 1~10:1~10:1~10:1~10, more preferably 2:1:2:1.
The fermentation temperature of three times described fermentations is 25 DEG C~28 DEG C, and its viscosity is: 4~5Pa s reduce when viscosity For: 3~4Pa s, complete three fermentations, fermentation time 4~7 days.
Described bacteria inactivation referred to: with 65~70 DEG C of sterilizings 20 minutes.
The present invention relates to the sugar-free meal enzyme liquid of compound generation that said method prepares, including: fluid matrix and additive, Wherein: fluid matrix is water and bacterium solution, additive be duck wheat powder of straw, green tea powder, scouring rush's powder, potentilla discolor powder, sweet wormwood powder, five Add skin powder, selfheal powder and generation meal agent.
Technique effect
Compared with prior art, what the sugar-free that the present invention prepares meal enzyme liquid of compound generation was possessed peculiar technology is imitated Fruit includes:
1) anti-hunger, reduction appetite: strengthening spleen and nourishing stomach, protect alimentary canal, drunk and will not starve;Note: send out owing to employing three times Ferment technology, by pectin, xanthans, guar gum in third time fermentation, Arabic gum has carried out short fermentation, can produce decomposition glue The colloid enzyme of body, such as pectase etc..And these pectases etc., it is beneficial to colloid sustained release is resolved into the materials such as glucose, So when human body takes sugar-free meal enzyme liquid of compound generation, human body can slowly absorb produced glucose during colloid decomposes, Reduce the hypoglycemic effect caused due to sugar-free generation meal enzyme liquid.And the mode of this sustained release can extend satiety, reduce famine Starving sense, increase can be for the meal time.Meanwhile, this sustained release fashion, it is more beneficial for diabetic, is not result in that blood glucose value is at once Raise.
2) enhance metabolism, accelerate blood circulation;
3) weight-losing toxin-expelling goes oedema: replace food, is suitable for jejunitas people to lose weight, wards off paddy toxin expelling crowd;
4) regulation endocrine;
5) immunoblastic lymphoma system is activated;
6) can the quickest jejunitas fat-reducing, reach fat-reducing effect according to average 0.4~0.6kg/ days loss of weight speed, the longest Jejunitas number of days is in the range of 12 days~30 days, and weight gain is inconspicuous after recovering diet, without continuing body weight growth trend.
Detailed description of the invention
Elaborating embodiments of the invention below, the present embodiment is carried out under premised on technical solution of the present invention Implement, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following enforcement Example.
Embodiment 1
The present embodiment meal enzyme liquid preparation method of compound generation is as follows:
1) one time fermentation: by 100 parts of water, 1 part of trichoderma reesei, 2 parts of Lactobacillus plantarums, 15 parts of glycerine, 5 parts of nicotinic acid, 10 parts Calcium monohydrogen phosphate and 10 parts of cholate mixing seal, and cultivate and activate and anaerobic fermentation, and fermentation temperature is 38.5 DEG C~39 DEG C, ferments 10 days Obtain ferment stoste;
Described trichoderma reesei uses commercially available trichoderma reesei.
Described Lactobacillus plantarum uses commercially available Lactobacillus plantarum powder.
2) in ferment stoste add additive, carry out secondary fermentation, fermentation temperature is 38.5 DEG C~40 DEG C, fermentation 15~ Take supernatant after 30 days, i.e. optimize enzyme liquid.
The content of described additive in terms of mass fraction, consisting of: 6 parts of duck wheat powder of straw, 5 parts of green tea powder, 2 parts Scouring rush's powder, 5 parts of potentilla discolor powder, 4 parts of sweet wormwood powder, 5 parts of cortex acanthopanacis powder and 3 parts of selfheal powder and 100 parts of ferment stostes;
3) optimization enzyme liquid is carried out ultrahigh-pressure sterilization inactivation, P=600MPa, 20 minutes duration, 3 times repeatedly.
4) the optimization enzyme liquid after ultrahigh-pressure sterilization inactivation and leavening, candida utili bacterium, water, glycerine and generation are obtained Meal agent mixing, sealing and fermenting, fermentation temperature is 25 DEG C~28 DEG C, and its viscosity is: 4~5Pa s are reduced to when viscosity: 3~ 4Pa s, completes three times and ferments, fermentation time 4~7 days,
Described candida utili bacterium uses commercially available candida utili bacterium.
The mass ratio of described leavening, candida utili bacterium, water, glycerine and generation meal agent is: 50 parts of sterilization inactivations Secondary fermentation enzyme liquid, 10 parts of candida utili bacterium, 50 parts of water, 15 parts of glycerine, 2 parts of generation meal agent.
The purity of above-mentioned glycerine, i.e. mass concentration ratio are 85%.
5) secondary bacterial inactivation, with 65~70 DEG C of sterilizings 20 minutes, i.e. obtains compound generation meal enzyme liquid.
Embodiment 2
The present embodiment meal enzyme liquid preparation method of compound generation is as follows:
1) one time fermentation: by 100 parts of water, 1 part of trichoderma reesei, 2 parts of Lactobacillus plantarums, 5 parts of glycerine, 5 parts of nicotinic acid, 1 part of phosphorus Acid hydrogen calcium and 1 part of cholate mixing seal, and cultivate and activate and anaerobic fermentation, and fermentation temperature is 38.5 DEG C~39 DEG C, ferments 10 days To ferment stoste;
2) in ferment stoste add additive, carry out secondary fermentation, fermentation temperature is 38.5 DEG C~40 DEG C, fermentation 15~ Take supernatant after 30 days, i.e. optimize enzyme liquid.
The content of described additive in terms of mass fraction, consisting of: 1 part of duck wheat powder of straw, 1 part of green tea powder, 1 part Scouring rush's powder, 1 part of potentilla discolor powder, 1 part of sweet wormwood powder, 1 part of cortex acanthopanacis powder and 1 part of selfheal powder and 50 parts of ferment stostes;
3) optimization enzyme liquid is carried out ultrahigh-pressure sterilization inactivation, P=600MPa, 20 minutes duration, 3 times repeatedly.
4) the optimization enzyme liquid after ultrahigh-pressure sterilization inactivation and leavening, candida utili bacterium, water, glycerine and generation are obtained Meal agent mixing, sealing and fermenting, fermentation temperature is 25 DEG C~28 DEG C, and its viscosity is: 4~5Pa s are reduced to when viscosity: 3~ 4Pa s, completes three times and ferments, fermentation time 4~7 days,
The mass ratio of described leavening, candida utili bacterium, water, glycerine and generation meal agent is: 50 parts of sterilization inactivations Secondary fermentation enzyme liquid, 10 parts of candida utili bacterium, 30 parts of water, 15 parts of glycerine, 2 parts of generation meal agent.
The purity of above-mentioned glycerine, i.e. mass concentration ratio are 85%.
5) secondary bacterial inactivation, with 65~70 DEG C of sterilizings 20 minutes, i.e. obtains compound generation meal enzyme liquid.
Reduction blood fat experimental study:
After test method is for carrying out 50 female hyperlipemia in mice of ICR taking 2 weeks for meal continuously, test display, this Invention ferment can reduce hyperlipidemia rats serum TG (triglycerides), TC (cholesterol) and improve HDL C (high density simultaneously Lipoprotein cholesterol) content, regulation blood fat is had good effects.
Number of days TG(mmol/L) TC(mmol/L) HDL‐C(mmol/L)
0 0.78±0.09 5.62±0.14 1.32±0.04
7 0.59±0.08 4.59±0.11 1.70±0.09
14 0.50±0.09 4.11±0.15 2.32±0.05
The experimental study of fat-reducing reduction body fat ratio:
After test method is for carrying out taking 2 weeks for meal continuously than obesity mice to 50 female high body fats of ICR, test is aobvious Showing, ferment of the present invention can reduce body fat ratio, reduction body weight weight-reducing has good effects.
Number of days Body weight (g) Body fat percentage %
0 31.97±2.09 33.62±1.14
7 28.59±0.08 31.59±0.91
14 27.50±0.09 30.61±1.05
Human efficiency tests:
1.1 laboratory sample
The meal enzyme liquid of compound generation taking above-mentioned gained is experiment product, makes one bottle with 3000 milliliters, and quality is 3.6kg.
1.2 experimental subjects
There are 8 employees (each 4 of men and women) of weight reducing wish as research object with Shanghai company, try receptor's working strength phase When slight manual labor, 29.18 ± 0.65 years old mean age, body weight is in 50kg~70kg.All experimenter's voluntary participations.
1.3 experimental technique
Experimenter, when starting the jejunitas phase, only allows to carry edible salt, water and one bottle 3 liters compound generation meal enzyme liquids, is hungry I.e. drink, do not starve and do not drink, slowly swallow.When 3 liters of compound generation meal enzyme liquids finish off or experimenter requires to terminate, and the jejunitas phase then terminates, Record the data such as the longest number of days and changes of weight simultaneously.
Summing up: loss of weight 0.511kg average every day in the jejunitas fat-reducing phase, drainage status confusion, hunger has, and energy state is high Putting forth energy, after recovering diet, in 5 days, body weight increases average 0.363kg, and within every 5 days, body weight increases variance yields 1.3376 × 10 3.Body weight without Substantially rebound trend.After 8 experimenters terminate the jejunitas phase, health check-up all represents healthy, without digestive tract ulcer.

Claims (8)

1. the preparation method of a compound generation meal enzyme liquid, it is characterised in that by the ferment stoste that will obtain after one time fermentation Secondary fermentation is carried out with medicinal material additive, then by the optimization enzyme liquid obtained and generation meal agent, candida utili bacterium and glycerine Carry out three fermentations after mixing, prepare compound generation meal enzyme liquid;
Described one time fermentation refers to: by 100 parts of water, 1 part of trichoderma reesei powder, 2 parts of Lactobacillus plantarum powder, 15 parts of glycerine, 5 parts of cigarettes Acid, 10 parts of calcium monohydrogen phosphates and the mixing of 10 parts of cholate seal, and then carrying out temperature is 38.5 DEG C ~ 40 DEG C, and the time is 7 ~ 14 days detest Aerobe fermentation, obtains ferment stoste;
Described secondary fermentation refers to: adds in ferment stoste and includes duck wheat powder of straw, green tea powder, scouring rush's powder, potentilla discolor The medicinal material additive of powder, sweet wormwood powder, cortex acanthopanacis powder or selfheal powder or a combination thereof, then carrying out temperature is 38.5 DEG C ~ 40 DEG C, time Between be 15 ~ 30 days anaerobic fermentations, take supernatant and i.e. optimize enzyme liquid.
Method the most according to claim 1, is characterized in that, consumption and the part by weight of described medicinal material additive be: bitter Buckwheat powder of straw 1 ~ 6 part, 1 ~ 5 part of green tea powder, 1 ~ 2 part of scouring rush's powder, 1 ~ 5 part of potentilla discolor powder, 1 ~ 4 part of sweet wormwood powder, cortex acanthopanacis powder 1 ~ 5 Part, 1 ~ 3 part of selfheal powder and ferment stoste 100 parts.
Method the most according to claim 1 and 2, is characterized in that, described duck wheat powder of straw, green tea powder, scouring rush's powder, turns over The mass ratio of white grass meal, sweet wormwood powder, cortex acanthopanacis powder or selfheal powder and ferment stoste is 6:5:2:5:4:5:3:100.
Method the most according to claim 1, is characterized in that, three times described fermentations refer to: surpassed by optimization enzyme liquid High pressure sterilization inactivates, and then mixes with candida utili bacterium, water, glycerine and generation meal agent and seals that then to carry out temperature be 25 DEG C ~ 28 DEG C, three fermentations of 4 ~ 7 days time, after completing, bacteria inactivation i.e. obtains compound generation meal enzyme liquid.
Method the most according to claim 4, is characterized in that, described optimization enzyme liquid, candida utili bacterium, water, sweet The part by weight of oil and generation meal agent is: 50:10:50:15:2.
6. according to the method described in claim 4 or 5, it is characterized in that, described generation meal agent includes: pectin, Arabic gum, xanthan Glue and guar gum, its mass ratio is 1 ~ 10:1 ~ 10:1 ~ 10:1 ~ 10.
7. according to the method described in claim 4 or 5, it is characterized in that, described generation meal agent includes: pectin, Arabic gum, xanthan Glue and guar gum, its mass ratio is: 2:1:2:1.
8. the sugar-free meal enzyme liquid of compound generation, it is characterised in that prepare according to method described in any of the above-described claim.
CN201510216016.0A 2015-04-30 2015-04-30 Sugar-free meal enzyme liquid of compound generation and preparation technology thereof Expired - Fee Related CN104783167B (en)

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CN105054017B (en) * 2015-07-30 2017-07-25 江西合善园国际食品实业有限公司 Ferment preparation method by raw material of plant placeula
CN107259523A (en) * 2016-04-07 2017-10-20 辽宁三元生态科技股份有限公司 The careless ferment of one kind file
CN106174522A (en) * 2016-07-28 2016-12-07 苏剑锋 A kind of Herba Artemisiae Annuae Flos Lonicerae ferment and preparation method thereof
CN107319283B (en) * 2017-06-29 2020-08-07 山东朱氏药业集团有限公司 Full-nutrition meal replacement powder containing fruit enzyme powder
CN111607534A (en) * 2020-05-08 2020-09-01 晶叶(银川)生物科技有限公司 Lactobacillus plantarum and application thereof in fruit and vegetable enzyme with constipation relieving effect
TWI817103B (en) * 2021-04-08 2023-10-01 利得美生技有限公司 Sugar-free cultured enzyme production process

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JP2007097553A (en) * 2005-10-07 2007-04-19 Life Maximizer 有限会社 Enzyme food, method for producing the same, seasoning containing the enzyme food and additive for alcoholic beverage
CN1994134A (en) * 2005-12-31 2007-07-11 天津市萌泉生物工程有限公司 Method for preparing weight-reducing food
CN103844266B (en) * 2014-03-25 2015-09-16 容瑜 A kind of preparation method of natural conditioning health ferment

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