CN105230782A - Preparation method of hair weed yoghourt - Google Patents
Preparation method of hair weed yoghourt Download PDFInfo
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- CN105230782A CN105230782A CN201510635720.XA CN201510635720A CN105230782A CN 105230782 A CN105230782 A CN 105230782A CN 201510635720 A CN201510635720 A CN 201510635720A CN 105230782 A CN105230782 A CN 105230782A
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Abstract
The invention discloses a preparation method of hair weed yoghourt. According to the preparation method, hair weed cells are cultured by a fluid suspension culture method so as to produce ultrafine hair weed cell powder, then ultrafine hair weed cell powder and hair weed extracellular polysaccharide are taken as the raw materials and added into fresh milk or reconstituted milk; at the same time, white granulated sugar, a stabilizing agent, and lactobacillus yoghurt starter are added to carry out lactic acid fermentation so as to prepare the hair weed yoghourt. The prepared hair weed yoghourt has the advantages of rich nutrients, unique flavor, and prominent healthcare functions.
Description
Technical field
The invention belongs to food processing technology field, be specifically related to a kind of preparation method of Yoghourt of delivering vegetables.
Background technology
Deliver vegetables also known as hair-like nostoc, be blue bacterium door nostoc object bacterium, its rich in nutritive value, has the history of more than one thousand years in China as a kind of edible cyanobacteria.Wild delivering vegetables is a kind of scattered algae at grassland sand-fixation plant periphery, long-term unplanned predation formula is gathered, not only make limited hair-like seaweed resource face exhaustion, also result in desertification, vegetation degeneration, bio-diversity minimizing simultaneously, cause huge destruction to ecological environment.From in July, 2000, the Chinese government will deliver vegetables and be classified as first-grade state protection plant, forbid gathering to wild delivering vegetables and selling.At present, hair weeds cells suspension culture techniques is very skillful, can adapt to carry out mass-produced needs.There are some researches show, exocellular polysaccharide of delivering vegetables has immunological regulation, antitumor, reducing blood lipid, hypoglycemic and antivirus action.Suspend cultivate cultivate hair weeds cells nutrient composition content than wild deliver vegetables higher, wherein suspending, it is higher than wild hair weeds cells by 18.2% to cultivate amino acid whose total content in hair weeds cells, ascorbic content is 131.3mg/100g, it is 3.8 times of wild hair weeds cells, the content of beta carotene, up to 6.42mg/g, is 2.8 times of wild hair weeds cells.
Yoghourt is a kind of health drink containing more lactic acid bacteria, can strengthen digestive function, promotes appetite, the metabolism of strengthening gastrointestinal peristalsis and body, often drinks the effect strengthening health.The carbohydrate of Yoghourt more easily digests, and protein is more conducive to the absorption of human body because forming fine grumeleuse, and its free amino acid amount increases by 4 times, and peptone and polypeptide obviously increase, and the metabolism of cholesterol and fat is better than cow's milk.Yoghourt is due to the fermentation of lactic acid bacteria, nutritional labeling is improved while, also some physiological activators are created as organic acid, aromatic substance, antibiotics, SOD, cell wall polysaccharides, organized enzyme, lactic acid bacteria MF and lactic acid bacteria etc., often drink and have significant regulating action to body function, reach the function of preventing and curing diseases.
Superfine communication technique is the new technology grown up over nearly 20 years, and so-called superfine communication technique refers to the process utilizing machinery or fluid dynamic approach material particles to be crushed to less than particle diameter 10-25 μm.Because material is ultrafine particle, its insoluble composition is longer because the impact of adhesive force excretes required time, and improve the absorptivity of active ingredient, uptake also can increase.
At present, market eating or dries and sell mainly with direct culinary art for the utilization of delivering vegetables greatly is master.Not only product type is single, the nutritional labeling in delivering vegetables also can be made to run off in a large number, reduce its edibility in processing procedure.On the market, also will not deliver vegetables and Yoghourt combines, by the proportioning of uniqueness with prepare that adding technology is prepared unique flavor, is of high nutritive value, the product of the evident in efficacy Yoghourt of delivering vegetables that keeps healthy occurs.
Summary of the invention
The technical problem to be solved in the present invention is: to suspend the obtained ultra micro hair weeds cells powder of the hair weeds cells cultivated and deliver vegetables exocellular polysaccharide for raw material with artificial eye-liquid, adopt unique fermentation technique, prepare the Yoghourt of delivering vegetables that a kind of nutritious, unique flavor, health care are evident in efficacy, to improve the value of delivering vegetables.
The present invention is for solving the problems of the technologies described above, and the technical scheme adopted is: a kind of preparation method of Yoghourt of delivering vegetables, and preparation process is:
The preparation of step one, ultra micro hair weeds cells powder
(1), aseptically, hair weeds cells is inoculated in the fluid nutrient medium in blake bottle, afterwards, blake bottle being placed on temperature is carry out cultivation 15 ~ 20 days in the constant temperature biochemical cultivation case of 25 DEG C, then, the nutrient solution of delivering vegetables obtained is carried out centrifugal filtration separation, collects the hair weeds cells and supernatant of delivering vegetables that obtain, for subsequent use;
(2), adopt clear water to carry out washing 3 ~ 5 times to the hair weeds cells that step (1) is collected, afterwards, freeze drying is carried out to the hair weeds cells after washing, then, adopt micronizer to be ground into ultra micro hair weeds cells powder, for subsequent use;
Step 2: the preparation of exocellular polysaccharide of delivering vegetables
(1) papain of its quality 0.5% is added in the supernatant of delivering vegetables, collected to step one, regulate the pH to 6.5 of gained mixed solution, and carry out enzymolysis 2h under being placed in 60 DEG C of temperature conditions, afterwards, carry out boiling water bath to go out ferment treatment 5min, then, centrifugation is carried out to gained mixed solution, get supernatant A, for subsequent use;
(2) be, the ratio of 4: 1 according to volume ratio, getting Sevage reagent joins in supernatant A, the mixed solution that gel precipitation is contained in lower floor is obtained after abundant concussion mixing, the albuminate of upper and lower two-layer intersection in removing mixed solution, repeatedly in mixed solution, add Sevage reagent, until upper and lower two-layer intersection gel-free precipitation in gained mixed solution, obtain de-protein solution, for subsequent use;
(3), the de-protein solution that step (2) obtains is placed in rotary evaporator carry out reduction vaporization concentrate, temperature when controlling reduced pressure concentration is 40-45 DEG C, until the content of solid content reaches 18 ° of Brix, afterwards, it is in the selective pellicle bag of 8000-10000 that the concentrate obtained is loaded molecular cut off, under magnetic agitation condition, adopts ultra-pure water to carry out dialysis treatment 48h, obtain polysaccharide liquid, for subsequent use;
(4) absolute ethyl alcohol is added in the polysaccharide liquid, obtained after step (3) dialysis treatment, afterwards, staticly settle 24h under the mixed solution obtained being placed in 4 DEG C of conditions, then, mixed solution is carried out centrifugal 10min under the rotating speed of 4000r/min, collect the lower sediment thing obtained, the ratio adding 1 ~ 3mL distilled water according to every gram of sediment is redissolved, and afterwards, carries out freeze drying to gained solution, must to deliver vegetables exocellular polysaccharide, for subsequent use;
The preparation of step 3, Yoghourt
(1), according to parts by weight, get 0.01 ~ 0.02 part, the ultra micro hair weeds cells powder that step one is obtained, the exocellular polysaccharide 0.0005 ~ 0.002 part of delivering vegetables that step 2 is obtained, white granulated sugar 6 ~ 8 parts, stabilizing agent 0.01 ~ 0.1 part, joining 85 ~ 90 parts of temperature is in the fresh milk of 40 ~ 50 DEG C or reconstituted milk, after being uniformly mixed, obtain mixed liquor, afterwards, mixed liquor is heated to 55-60 DEG C, and to be placed in pressure be that the homogenizer of 15-20MPa carries out homogeneous process 2-3 time, then, sterilization processing is carried out to the mixed liquor after homogeneous, 40 ~ 45 DEG C are cooled to after sterilization processing, obtain mixed emulsion, for subsequent use,
(2), according to the inoculum concentration of 3 ~ 6%, lactic acid fermenting bacteria is inoculated in above-mentioned obtained mixed emulsion, afterwards, ferment under being placed in 40 ~ 45 DEG C of temperature conditions, until when the acidity of mixed emulsion reaches 70 ° of T ~ 80 ° T, transposition carries out refrigeration 12 ~ 24h in the refrigerator of 0 ~ 4 DEG C, then, aseptically carry out filling, get product Yoghourt of delivering vegetables.
In step 2, described Sevage reagent due to volume ratio be 5: 1 chloroform and n-butanol form.
In step 2, in the process of described dialysis treatment, every 3h changes a water.
In step 3, described stabilizing agent is at least one in pectin, gelatin, carrageenan, your bean gum lonely, carboxymethyl cellulose, xanthans, sodium alginate, single sweet intoxicated, sucrose vinegar.
In step 3, described lactic acid fermenting bacteria is at least one in lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, Bifidobacterium, Lactobacillus plantarum.
In step 3, the temperature of described sterilization processing is 80 ~ 85 DEG C, and the time is 5 ~ 10min.
Beneficial effect:
1, the preparation method of a kind of Yoghourt of delivering vegetables provided by the invention, method is simple to operate, utilization of materials is high, the comprehensive allotment of hair weeds cells powder and exocellular polysaccharide of delivering vegetables utilizes, not only increase the comprehensive utilization ratio of delivering vegetables, abundant, complete for nutritional labeling in delivering vegetables is added in Yoghourt, has improved local flavor and the mouthfeel of Yoghourt, improve the value of delivering vegetables.Complicated biochemical reaction can also be there is during the fermentation in the nutritional labelings such as the Nostoc flagelliforme Polysaccharides of delivering vegetables, protein, phycocolloid with the organic acid in Yoghourt, organized enzyme and aromatic substance etc., promote to deliver vegetables the Small molecular of Middle nutrition material, and the reservation of medicinal ingredient and multiple bioenzyme activity, nutriment is not only made more easily to be absorbed by the body, also play and improve body immunity, antitumor, reducing blood lipid, hypoglycemic and antiviral, oxidation resistant effect, the effect promoted health.
2, in the present invention, the drying of hair weeds cells and exocellular polysaccharide solution of delivering vegetables adopts cryodesiccated method, and this technology also can be dewatered relatively more thorough to heat sensitive material, and the sample of drying is very stable, is convenient to long time stored.Because the drying of material completes in the frozen state, compared with other drying means, physical arrangement and the molecule structure change of material are minimum, its institutional framework and mode of appearance are preserved preferably, reduce the destructive rate of hair weeds cells constituent, keep the original nutriment of hair weeds cells and local flavor preferably.
3, the present invention takes full advantage of the hair weeds cells in cultivating and the exocellular polysaccharide in nutrient solution of suspending of delivering vegetables, and improves the utilization rate that hair weeds cells is cultivated.
4, the ultra micro hair weeds cells powder added in the present invention, makes the specific area increase at double of hair weeds cells, improves its biologically active and absorptivity, and can improve the mouthfeel of Yoghourt, improves the nutritive value of Yoghourt.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is further elaborated.
A kind of preparation method of Yoghourt of delivering vegetables, this preparation method is with the obtained ultra micro hair weeds cells powder of liquid suspension culture hair weeds cells and deliver vegetables exocellular polysaccharide for raw material, add in fresh milk or reconstituted milk, add white granulated sugar, stabilizing agent, lactobacillus yogurt leavening simultaneously, obtain through lactic fermentation.Specifically comprise the following steps:
The preparation of step one, ultra micro hair weeds cells powder
(1), aseptically, hair weeds cells is inoculated in the fluid nutrient medium in blake bottle, afterwards, blake bottle being placed on temperature is carry out cultivation 15 ~ 20 days in the constant temperature biochemical cultivation case of 25 DEG C, then, the nutrient solution of delivering vegetables obtained is carried out centrifugal filtration separation, collects the hair weeds cells and supernatant of delivering vegetables that obtain, for subsequent use;
(2), adopt clear water to carry out washing 3 ~ 5 times to the hair weeds cells that step (1) is collected, afterwards, freeze drying is carried out to the hair weeds cells after washing, then, adopt micronizer to be ground into ultra micro hair weeds cells powder, for subsequent use;
Step 2: the preparation of exocellular polysaccharide of delivering vegetables
(1) papain of its quality 0.5% is added in the supernatant of delivering vegetables, collected to step one, regulate the pH to 6.5 of gained mixed solution, and carry out enzymolysis 2h under being placed in 60 DEG C of temperature conditions, afterwards, carry out boiling water bath to go out ferment treatment 5min, then, centrifugation is carried out to gained mixed solution, get supernatant A, for subsequent use;
(2) be, the ratio of 4: 1 according to volume ratio, get Sevage reagent (chloroform: n-butanol=5: 1) join in the obtained supernatant A of step (1), the mixed solution that gel precipitation is contained in lower floor is obtained after abundant concussion mixing, the albuminate of upper and lower two-layer intersection in removing mixed solution, repetition like this is till intersection gel-free precipitation, obtain de-protein solution, for subsequent use;
(3), the de-protein solution that step (2) obtains is placed in rotary evaporator carry out reduction vaporization concentrate, temperature when controlling reduced pressure concentration is 40-45 DEG C, until the content of solid content reaches 18 ° of Brix, afterwards, it is in the selective pellicle bag of 8000-10000 that the concentrate obtained is loaded molecular cut off, dialyses 48 hours by means of magnetic stirring apparatus ultra-pure water, within every three hours, change a water, removing Small molecular, obtains polysaccharide liquid, for subsequent use;
(4), the polysaccharide liquid obtained after step (3) dialysis treatment is poured into after in conical flask, and add a certain amount of absolute ethyl alcohol, afterwards, 24h is staticly settled under the mixed solution obtained being placed in 4 DEG C of conditions, then, after mixed solution is taken out from refrigerator, centrifugal 10min is carried out under the rotating speed of 4000r/min, collect the lower sediment thing obtained, the ratio adding 1 ~ 3mL distilled water according to every gram of sediment carries out redissolving in ultra-pure water, afterwards, pour gained solution into flat board, wrap with preservative film, after carrying out freeze drying, must to deliver vegetables exocellular polysaccharide, for subsequent use,
The preparation of step 3, Yoghourt
(1), according to parts by weight, get 0.01 ~ 0.02 part, the ultra micro hair weeds cells powder that step one is obtained, the exocellular polysaccharide 0.0005 ~ 0.002 part of delivering vegetables that step 2 is obtained, white granulated sugar 6 ~ 8 parts, stabilizing agent 0.01 ~ 0.1 part, joining 85 ~ 90 parts of temperature is in the fresh milk of 40 ~ 50 DEG C or reconstituted milk, after being uniformly mixed, obtain mixed liquor, afterwards, mixed liquor is heated to 55-60 DEG C, and to be placed in pressure be that the homogenizer of 15-20MPa carries out homogeneous process 2-3 time, then, sterilization processing is carried out to the mixed liquor after homogeneous, 40 ~ 45 DEG C are cooled to after sterilization processing, obtain mixed emulsion, for subsequent use,
(2), according to the inoculum concentration of 3 ~ 6%, lactic acid fermenting bacteria is inoculated in the obtained mixed emulsion of step (1), afterwards, ferment under being placed in 40 ~ 45 DEG C of temperature conditions, until when the acidity of mixed emulsion reaches 70 ° of T ~ 80 ° T, transposition carries out refrigeration 12 ~ 24h in the refrigerator of 0 ~ 4 DEG C, then, aseptically carry out filling, get product Yoghourt of delivering vegetables.
In step 3, described stabilizing agent is at least one in pectin, gelatin, carrageenan, your bean gum lonely, carboxymethyl cellulose, xanthans, sodium alginate, single sweet intoxicated, sucrose vinegar.
In step 3, described lactic acid fermenting bacteria is at least one in lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, Bifidobacterium, Lactobacillus plantarum.
In step 3, the temperature of described sterilization processing is 80 ~ 85 DEG C, and the time is 5 ~ 10min.
Claims (6)
1. deliver vegetables the preparation method of Yoghourt, and it is characterized in that, preparation process is as follows:
The preparation of step one, ultra micro hair weeds cells powder
(1), aseptically, hair weeds cells is inoculated in the fluid nutrient medium in blake bottle, afterwards, blake bottle being placed on temperature is carry out cultivation 15 ~ 20 days in the constant temperature biochemical cultivation case of 25 DEG C, then, the nutrient solution of delivering vegetables obtained is carried out centrifugal filtration separation, collects the hair weeds cells and supernatant of delivering vegetables that obtain, for subsequent use;
(2), adopt clear water to carry out washing 3 ~ 5 times to the hair weeds cells that step (1) is collected, afterwards, freeze drying is carried out to the hair weeds cells after washing, then, adopt micronizer to be ground into ultra micro hair weeds cells powder, for subsequent use;
Step 2: the preparation of exocellular polysaccharide of delivering vegetables
(1) papain of its quality 0.5% is added in the supernatant of delivering vegetables, collected to step one, regulate the pH to 6.5 of gained mixed solution, and carry out enzymolysis 2h under being placed in 60 DEG C of temperature conditions, afterwards, carry out boiling water bath to go out ferment treatment 5min, then, centrifugation is carried out to gained mixed solution, get supernatant A, for subsequent use;
(2) be, the ratio of 4: 1 according to volume ratio, getting Sevage reagent joins in supernatant A, the mixed solution that gel precipitation is contained in lower floor is obtained after abundant concussion mixing, the albuminate of upper and lower two-layer intersection in removing mixed solution, repeatedly in mixed solution, add Sevage reagent, until upper and lower two-layer intersection gel-free precipitation in gained mixed solution, obtain de-protein solution, for subsequent use;
(3), the de-protein solution that step (2) obtains is placed in rotary evaporator carry out reduction vaporization concentrate, temperature when controlling reduced pressure concentration is 40-45 DEG C, until the content of solid content reaches 18 ° of Brix, afterwards, it is in the selective pellicle bag of 8000-10000 that the concentrate obtained is loaded molecular cut off, under magnetic agitation condition, adopts ultra-pure water to carry out dialysis treatment 48h, obtain polysaccharide liquid, for subsequent use;
(4) absolute ethyl alcohol is added in the polysaccharide liquid, obtained after step (3) dialysis treatment, afterwards, staticly settle 24h under the mixed solution obtained being placed in 4 DEG C of conditions, then, mixed solution is carried out centrifugal 10min under the rotating speed of 4000r/min, collect the lower sediment thing obtained, the ratio adding 1 ~ 3mL distilled water according to every gram of sediment is redissolved, and afterwards, carries out freeze drying to gained solution, must to deliver vegetables exocellular polysaccharide, for subsequent use;
The preparation of step 3, Yoghourt
(1), according to parts by weight, get 0.01 ~ 0.02 part, the ultra micro hair weeds cells powder that step one is obtained, the exocellular polysaccharide 0.0005 ~ 0.002 part of delivering vegetables that step 2 is obtained, white granulated sugar 6 ~ 8 parts, stabilizing agent 0.01 ~ 0.1 part, joining 85 ~ 90 parts of temperature is in the fresh milk of 40 ~ 50 DEG C or reconstituted milk, after being uniformly mixed, obtain mixed liquor, afterwards, mixed liquor is heated to 55-60 DEG C, and to be placed in pressure be that the homogenizer of 15-20MPa carries out homogeneous process 2-3 time, then, sterilization processing is carried out to the mixed liquor after homogeneous, 40 ~ 45 DEG C are cooled to after sterilization processing, obtain mixed emulsion, for subsequent use,
(2), according to the inoculum concentration of 3 ~ 6%, lactic acid fermenting bacteria is inoculated in above-mentioned obtained mixed emulsion, afterwards, ferment under being placed in 40 ~ 45 DEG C of temperature conditions, until when the acidity of mixed emulsion reaches 70 ° of T ~ 80 ° T, transposition carries out refrigeration 12 ~ 24h in the refrigerator of 0 ~ 4 DEG C, then, aseptically carry out filling, get product Yoghourt of delivering vegetables.
2. the preparation method of a kind of Yoghourt of delivering vegetables as claimed in claim 1, is characterized in that: in step 2, described Sevage reagent due to volume ratio be 5: 1 chloroform and n-butanol form.
3. the preparation method of a kind of Yoghourt of delivering vegetables as claimed in claim 1, it is characterized in that: in step 2, in the process of described dialysis treatment, every 3h changes a water.
4. the preparation method of a kind of Yoghourt of delivering vegetables as claimed in claim 1, it is characterized in that: in step 3, described stabilizing agent is at least one in pectin, gelatin, carrageenan, your bean gum lonely, carboxymethyl cellulose, xanthans, sodium alginate, single sweet intoxicated, sucrose vinegar.
5. the preparation method of a kind of Yoghourt of delivering vegetables as claimed in claim 1, it is characterized in that: in step 3, described lactic acid fermenting bacteria is at least one in lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, Bifidobacterium, Lactobacillus plantarum.
6. the preparation method of a kind of Yoghourt of delivering vegetables as claimed in claim 1, it is characterized in that: in step 3, the temperature of described sterilization processing is 80 ~ 85 DEG C, and the time is 5 ~ 10min.
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Cited By (5)
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| CN106578074A (en) * | 2016-12-15 | 2017-04-26 | 青海金祁连乳业有限责任公司 | Health preserving fruit yogurt and manufacture method thereof |
| CN106912602A (en) * | 2017-01-24 | 2017-07-04 | 深圳市大百汇技术有限公司 | A kind of fermented dairy by Lactobacillus acidophilus of laetarius volemus polysaccharide and preparation method thereof |
| CN107019043A (en) * | 2017-03-22 | 2017-08-08 | 华南理工大学 | A kind of Lactobacillus plantarum acidified milk of laetarius volemus polysaccharide and preparation method thereof |
| CN107047762A (en) * | 2017-03-22 | 2017-08-18 | 华南理工大学 | A kind of lactobacillus casei fermented milk of laetarius volemus polysaccharide and preparation method thereof |
| CN110235945A (en) * | 2019-07-18 | 2019-09-17 | 江西阳光乳业股份有限公司 | A kind of fermentation process of Yoghourt |
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