CN103571767B - Utilize double application lactobacillus powder of edible agar and preparation method thereof - Google Patents
Utilize double application lactobacillus powder of edible agar and preparation method thereof Download PDFInfo
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- CN103571767B CN103571767B CN201210262911.2A CN201210262911A CN103571767B CN 103571767 B CN103571767 B CN 103571767B CN 201210262911 A CN201210262911 A CN 201210262911A CN 103571767 B CN103571767 B CN 103571767B
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Abstract
The present invention relates to lactobacillus powder and preparation method thereof and the product comprising this powder.Described lactobacillus powder utilizes edible agar to make the lactobacillus powder that the matrix structure of the double application milk-acid bacteria be made up of protein and polyose (bacterium block) is firmer.According to the present invention, the double application lactobacillus powder of the protein and polyose that comprise edible agar shows the acid resistance of enhancing, bile-tolerance and accelerated test stability, can effectively for the preparation of multiple lactobacteria-containing product.
Description
Technical field
The present invention relates to the double application lactobacillus powder (powder) utilizing edible agar (Agar), more specifically, relate to a kind of double application lactobacillus powder utilizing edible agar and preparation method thereof, described utilization eats the double application lactobacillus powder of agar, edible agar protein and the milk-acid bacteria of polyose composition to fermentation is utilized to carry out double application, thus form the bacterium block of firm matrix (matrix) (grid) form, thus show the acid resistance of enhancing, bile-tolerance and accelerated stability.
Background technology
Milk-acid bacteria (lacticacidbacteria) is otherwise known as lactic-acid-bacterium or lactic acid bacteria, and it is perched in mammiferous intestines, and preventing because of assorted microbial abnormal fermentation, is the useful bacterium being used as digester.Such as, lactobacillus bulgaricus (L.bulgaricus) is the earliest by well-known milk-acid bacteria, and it is for the preparation of Yoghourt, when preparing cheese or fermentation missible oil, is also used as starter.In addition, aerobic Bacterium lacticum (L.acidophilus) is present in people and all mammals, and in the intestines of all the other animals.Also for the preparation of cream or milk, and self poisoning in treatment intestines.In addition, Lactococcus lactis (L.lactis) generates DL-LACTIC ACID, is usually present in milk, thus for the preparation of cream or cheese, is most important bacterium at Dairy Products Industry Implementing.Meanwhile also have bifidus, especially common in baby's intestines of edible breast milk, its quantity shows along with adult the trend reduced gradually, is therefore considered to very important to the health in infancy.In addition; the same with milk-acid bacteria; the organic acid and the antibacterial substance that generate tunning show effect in suppression intestinal toxic bacterium; recently for utilizing the research of the lactic acid bacteria fermentation milk of bifidus and the health in order to strengthen the child eating breast milk, joined the researchs such as the commercialization in milk powder very active.
As mentioned above, useful milk-acid bacteria rests in intestines, play multiple physiologically active effect, as activated intestinal tube motion, suppressing harmful bacteria, promoting VITAMIN and strengthen phylactic agent etc., but in the structure of human body, when people absorbs milk-acid bacteria, milk-acid bacteria can be dead because of hydrochloric acid in gastric juice or bile acide, thus there is many times milk-acid bacteria and can not play the situation of original physiological active functions.
In order to solve the problem, have developed the technology etc. with protein and polyose double application milk-acid bacteria.
Particularly; the milk-acid bacteria double application technology of above-mentioned protein and polyose; it is characterized by: in the substratum be made up of sugared composition and ion component; cultivate milk-acid bacteria; when reclaiming lactic acid thalline; in milk-acid bacteria concentrated aqueous solution, comprise cryoprotectant, be coated with protein and polyose.The milk-acid bacteria double application technology of above-mentioned existing protein and polyose, it is by being coated with milk-acid bacteria protein and polyose, milk-acid bacteria can not be exposed in air, therefore enhance the thermotolerance of milk-acid bacteria, acid resistance and bile-tolerance, thus can expect viable bacteria stability and the improved effect of processing stability.
But, the above-mentioned existing milk-acid bacteria coating technique improved, although it is suitable for the lactobacillus product of existing powder morphology, but in food that moisture content is many etc., for the death preventing the milk-acid bacteria caused because of moisture, sufficient effect can not be demonstrated, therefore, compared with numerous food varieties, be noted and still there is following problem: its range of application can only be limited.
For this reason, the present invention is in order to strengthen the bonding force of protein and polyose further, disclose a kind of double application lactobacillus powder using edible agar, described double application lactobacillus powder forms firm bacterium block, and described bacterium block is made up of milk-acid bacteria and protein, polyose, edible agar.
Summary of the invention
The technical problem solved
The object of the invention is to, there is provided a kind of by using protein and polyose to carry out double application to milk-acid bacteria, utilize edible agar (Agar) to strengthen the bonding force of the bacterium block formed by protein and polyose, milk-acid bacteria, thus demonstrate the lactobacillus powder of excellent acid resistance, bile-tolerance, accelerated stability.
Another object of the present invention is to, provide a kind of comprise above-mentioned edible agar, the preparation method of the double application lactobacillus powder of protein and polyose.
Another object of the present invention is, provide a kind of comprise above-mentioned edible agar, the product of the double application lactobacillus powder of protein and polyose.
Technical scheme
As an embodiment, the present invention relates to and comprise edible agar, protein and polyose composition, and carried out the lactobacillus powder that is coated with by the coating layer forming bacterium block.
Double application lactobacillus powder of the present invention like this, it is preferably prepared by the operation comprising following step.In the substratum for cultivating milk-acid bacteria, adding edible agar, then fermenting, form milk-acid bacteria concentrated aqueous solution;
Protein and polyose composition is added in above-mentioned milk-acid bacteria concentrated aqueous solution; And
To above-mentioned add edible agar, protein, polyose composition milk-acid bacteria concentrated aqueous solution carry out lyophilize.
Below, formation of the present invention is described in detail.
In order to prepare double application lactobacillus powder of the present invention, first implement in the substratum for cultivating milk-acid bacteria, to add edible agar, the operation of then fermenting.
As being applicable to milk-acid bacteria of the present invention, such as can usage chain Coccus (Streptococcus), lactococcus (Lactococcus), enterococcus spp (Enterococcus), lactobacillus (Lactobacillus), Pediococcus (Pediococcus), Leuconostoc (Leuconostoc), Wei Si Bordetella (Weissella) and genus bifidobacterium (Bifidobacterium).
In addition, for cultivate milk-acid bacteria substratum in comprise sugared composition, ion component, protein component and yeast extract.Glucose, lactose, fructose, sucrose, maltose etc. can be utilized as sugared composition, ammonium citrate, sodium-acetate, Rhodiaphos DKP, magnesium sulfate, manganous sulfate, sodium-chlor etc. can be utilized as ion component, rice protein, soybean protein isolate, casein peptone (caseinpeptone), soy peptone (soypeptone), skim-milk, whey-protein etc. can be utilized as protein ingredient, but be not limited to these.
And, fermentation lactic acid bacteria in the aqueous solution of protein ingredient comprising the edible agar of 0.1% ~ 5.0% scope, the sugared composition of 0.1% ~ 10% scope, the yeast extract of 0.1% ~ 10% scope, the ion component of 0.001% ~ 2.0% scope and 0.01% ~ 20% scope, thus prepare streptococcus acidi lactici fermented solution.
When the add-on of edible agar is more than 5.0%, the intensity of jelly is high, and liquid nutrient medium therefore can not be used to cultivate milk-acid bacteria.During the quantity not sufficient 0.1% added, form jelly faint, not display effect.In addition, when the add-on of sugared composition is more than 10%, produces osmotic pressure phenomenon, therefore do not cultivate milk-acid bacteria, if add quantity not sufficient 0.1% time, due to the deficiency of energy source, the problem can not cultivating milk-acid bacteria can be there is.Further, when the add-on of yeast extract is more than 10%, also can not demonstrate special effect, but during less than 0.1%, can milk-acid bacteria do not cultivated.Further, when ion component is more than 2%, generation problem in transportation of substances aspect inside and outside lactic-acid bacteria cells, thus do not cultivate milk-acid bacteria, during less than 0.001%, do not show the effect adding ion component.Further, when protein ingredient is more than 20%, the solubleness of protein reduces, and the problem increased can occur, during less than 0.01%, can not show effect with the protein that insoluble state exists.
In addition, above-mentioned edible agar (Agar) can be passed through from the red algae such as gelidium or fragrant plant mentioned in ancient texts, with high-temperature boiling, gelatinoid is melted in water, filter, remove after solid matter, repeatedly carry out solidifying, freezing, dehydration, the process such as dry obtain, the main component of the edible agar formed in this way is made up of agarose (Agarose) and agaropectin (Agaropectin).Then, edible agar is blended in the solution of acidity between pH5 to pH7, after boiling, cool, start gelation (Gelification) at about 40 DEG C, form jelly, such glue eats agar when lyophilize, along with the water constituent of jelly inside is removed, there is film forming character.
Therefore, of the present inventionly to be constructed as follows, above-mentioned edible agar is used as lactobacillus-fermented substratum, under nutrient solution state, with edible agar jelly morphosis lactobacillus-fermented, with protein and polyose, the milk-acid bacteria concentrated solution comprising milk-acid bacteria and edible agar jelly is coated with after making.Then, during lyophilize, along with edible agar forms film morphology, make double application matrix (grid) form of protein and polyose firmer, thus suppress moisturizing moisture, more improve thermotolerance, acid resistance, bile-tolerance, viable bacteria stability and accelerated stability can be strengthened.
And, for the aqueous solution comprising edible agar, sugared composition and ion component, at the temperature of 120 DEG C ~ 130 DEG C of scopes after sterilizing, when cooling, along with edible agar gelation from 40 DEG C (Gelification), form edible agar jelly, so edible agar jelly and milk-acid bacteria are together fermented, then form the streptococcus acidi lactici fermented solution comprising edible agar jelly.In addition, for the above-mentioned aqueous solution comprising edible agar, sugared composition and ion component, at the temperature more than 130 DEG C when sterilizing, there is carbonization phenomenon, thus milk-acid bacteria can be made to cultivate have problems, in addition, at less than 120 DEG C when sterilizing, sterilising effect reduces, thus can produce microbiological contamination problem.
Then, by implementing above-mentioned streptococcus acidi lactici fermented solution to be separated and concentrated operation, thus the milk-acid bacteria concentrated aqueous solution comprising edible agar jelly is formed.By such concentration process, milk-acid bacteria is concentrated into 10
10~ 10
11during CFU/mL, then along with edible agar jelly and milk-acid bacteria coprecipitation, thus effective coating can be induced.Such as, after lactobacillus-fermented, utilize continuous centrifugal separator, thalline is separated and concentrates, then can obtain edible agar jelly and the milk-acid bacteria concentrated aqueous solution of precipitation.
Further, when adding protein component to be coated with thalline in above-mentioned milk-acid bacteria concentrated solution, then edible agar and protein coating can be formed.Edible agar like this and protein coating can give milk-acid bacteria thermotolerance, acid resistance and bile-tolerance.
Adding of above-mentioned protein component can have been come by the mode adding protein water soln in the milk-acid bacteria concentrated aqueous solution of precipitation.The protein concn of above-mentioned protein water soln can in the scope of 0.1% ~ 20%.As protein, rice protein, soybean protein isolate, soy peptone, casein peptone, skim-milk, whey-protein can be used alone or as a mixture, but be not limited to these.At this, when above-mentioned protein protein concn is in aqueous more than 20%, the solubleness of protein reduces, and the protein existed with insoluble state can be made to increase, if less than 0.1% time, can not show Painting effect.
Polyose composition is added above-mentioned adding in the milk-acid bacteria concentrated aqueous solution of protein component.Adding of above-mentioned polyose composition in the present invention can adopt the mode adding the polyose aqueous solution in milk-acid bacteria and protein mixed aqueous solution to complete.The above-mentioned polyose aqueous solution is preferably that 0.1% ~ 30% scope adds with polyose concentration.Because during more than 30%, the viscosity of the polyose aqueous solution is high, when sterilizing, caramelization occurs, thus is difficult to use, and during less than 0.1%, can not show Painting effect.
Such polyose can be used alone or as a mixture carrageenin (Carrageenan), xanthan gum (Xanthangum), gelling gum (Gellangum), guar gum (Guargum), Curdlan (Curdlan), Sudan Gum-arabic (Arabicgum), algin (Algin), POLY-karaya (Karayagum), tragacanth gum (Tragacanthgum), India(n) gum (Ghattigum), Viscogum BE (Locustbeangum), tamarind seed gum (Tamarindgum), plantasan (Psylliumseedgum), pulullan polysaccharide (Pullulan), Wei Lan glue (Welan), sandlwood glue (Rhamsan), pectin (Pectin), Xylo-Mucine (CMC), Polylevulosan (Levan), sorbyl alcohol (Sorbitol), Xylitol (Xylitol), inositol (Inositol), erythritol (Erythritol), methylcellulose gum (Methylcellulose), Star Dri 5 (Maltodextrin), glucomannan (Glucomannan), xylogen (lignin) etc., but be not limited to these.
In addition, described polyose can according to comprise edible agar and protein component milk-acid bacteria concentrated aqueous solution gross weight 0.001% ~ 10% weight add.When the add-on of polyose is more than 10%, producing can not the problem of even spread, during less than 0.1%, can not show Painting effect, therefore preferably joins in milk-acid bacteria concentrated aqueous solution in above-mentioned scope.
As mentioned above, make milk-acid bacteria applied by polyose, the strong bonding force had by polyose, produce the combination between thalline, thus the very fine and close bacterium block of structure can be formed.Especially when polyose is aqueous solution state, under the condition of acidity, solubleness is extremely low, and easy dissociation and stripping under the pH more than neutrality, therefore can strengthen stability and the intestines internal fixtion of milk-acid bacteria in acid condition.
And; in the present invention, lyophilize is carried out to above-mentioned milk-acid bacteria, thus storage stability can be improved further, for this reason; except adding for the formation of except the protein of double application and polyose composition in the milk-acid bacteria concentrated aqueous solution containing edible agar, add cryoprotectant further.Now; described cryoprotectant can add all to add for the formation of the protein of double application and polyose composition after add; or double application composition and cryoprotectant are together added, but add double application composition again after preferably first adding cryoprotectant in milk-acid bacteria concentrated aqueous solution.
After adding cryoprotectant like this, adopt usual method to implement freezing dry process, then milk-acid bacteria can not be dead in freezing dry process.Above-mentioned cryoprotectant can use the polysaccharide such as trehalose, Star Dri 5, N.F,USP MANNITOL, sorbyl alcohol, can use the monose such as glucose, fructose, maltose, sucrose.In addition, also whey powder, starch and skim-milk can be used.
In addition; because the solubleness of cryoprotectant raw material is no more than 50%; if when therefore the add-on of cryoprotectant makes concentration more than 50%; can exist with insoluble state; during less than 0.1%; can not show the effect of cryoprotectant, therefore the concentration of cryoprotectant preferably adopts the aqueous solution state of 1 ~ 50% scope.
In addition, described cryoprotectant also can with relative to milk-acid bacteria concentrated aqueous solution gross weight 1 ~ 50% add-on add use.During relative to the add-on of milk-acid bacteria concentrated aqueous solution cryoprotectant more than 50%, can not mix equably, can not cryoprotective effect be shown, according to when carrying out adding less than 1%, can not show the effect of cryoprotectant.
As another embodiment, the invention provides and a kind of there is the preparation method comprising the lactobacillus powder of the bacterium block structured coating layer of protein and polyose composition comprising above-mentioned edible agar.The feature of above-mentioned preparation method is for comprising the following steps: (a) adds edible agar in the substratum for cultivating milk-acid bacteria, then ferments, and forms milk-acid bacteria concentrated aqueous solution; B () adds protein and polyose composition in above-mentioned milk-acid bacteria concentrated aqueous solution; (c) to above-mentioned add edible agar, protein, polyose composition milk-acid bacteria concentrated aqueous solution carry out lyophilize.
At this, in the substratum for milk-acid bacteria cultivation in step (a), sugared composition, ion component, protein component and yeast extract etc. can be comprised.Further, in described step (b), protein and polyose composition can add in a certain order, or can add simultaneously.
In addition, preparation in accordance with the present invention, in described step (b), can also add cryoprotectant composition in milk-acid bacteria concentrated aqueous solution.
As another embodiment, the present invention goes for the product of the lactobacillus powder utilizing above-mentioned preparation method to obtain, and these products comprise fermented-milk, modified milk, sauce class leavened food, pickle fermentation food, functional drinks, functional foodstuff, normal food, pharmaceuticals and makeup etc.
Utilization of the present invention eats the double application lactobacillus powder of agar, its acid resistance, bile-tolerance, excellent heat resistance, and accelerated stability is high, when by human intake, the dead degree of milk-acid bacteria caused because of hydrochloric acid in gastric juice, bile acide is low, thus there is the feature that can maintain the original physiological active functions of milk-acid bacteria for a long time, therefore can be effectively applied to using lactic bacteria activity as in required numerous food.
The effect of invention
Utilization according to the present invention eats the double application lactobacillus powder of agar, and it has acid resistance, bile-tolerance is high, the advantage that the accelerated test stability under extreme environment is high.In addition, when absorbing milk-acid bacteria, because of hydrochloric acid in gastric juice, bile acide and dead, can not have the advantages that to lose the original physiological active functions of milk-acid bacteria.
Therefore, double application lactobacillus powder according to the present invention can be effectively applied to using lactic bacteria activity as required fermented-milk, in the preparation of modified milk, sauce class leavened food, pickle fermentation food, functional drinks, functional foodstuff, normal food and makeup etc.
Embodiment
Below, by the preferred embodiments of the present invention, formation of the present invention and effect are given the account in greater detail.But following embodiment just understands the present invention to contribute to, and scope of the present invention is not limited in the following example.In the content that this does not record, to those skilled in the art, can technology presumption be carried out completely, therefore omit the explanation to this partial content.
Embodiment 1 utilizes the preparation of the double application lactobacillus powder of edible agar
First, in order to produce lactobacterium casei (LactobacilluscaseiCBT-LC) milk-acid bacteria be coated with separately with protein, following experiment has been carried out.
Particularly, add 5% glucose as sugared composition, 2% yeast extract, 3% soy peptone, 0.1% edible agar, 0.2% potassium primary phosphate, 0.2% ammonium citrate, 0.2% sodium-acetate, 0.01% magnesium sulfate, as ion component, then make them dissolve, and transfer in the anaerobic fermentation pipe of 200L capacity, sterilizing in 15 minutes is carried out at 121 DEG C, inoculation 2L bacterial classification, maintains pH with ammonia, ferments 12 hours.After above-mentioned fermentation, with the flow velocity of 60L/hr, fermented liquid is transferred in continuous centrifugal separator, make thalline and edible agar jelly precipitation, thus define milk-acid bacteria concentrated aqueous solution.In above-mentioned milk-acid bacteria concentrated aqueous solution, add 0.5% soybean protein isolate as protein component, thus generate lactobacterium casei (LactobacilluscaseiCBT-LC) milk-acid bacteria with edible agar and protein component coating.
Then, join above-mentioned being coated with in the milk-acid bacteria aqueous solution of described protein mix being used for the cryodesiccated cryoprotectant of milk-acid bacteria.Particularly, by the trehalose of 30%, 20% the sorbyl alcohol cryoprotectant aqueous solution carry out pressurization sterilizing, in above-mentioned obtained milk-acid bacteria concentrated aqueous solution, add cryoprotectant, the speed with 5,000rpm in stirrer stirs, and carries out the process that homogenizes.
Then, in order to produce lactobacterium casei (LactobacilluscaseiCBT-LC) milk-acid bacteria carrying out double application with protein and polyose, following experiment has been carried out further.
To mixing 1% xanthan gum, 1% Xylo-Mucine, the sterilizing and the 3L polyose aqueous solution dissolved enforcement is pressurizeed of 1% sorbyl alcohol, thus the obtained polyose aqueous solution, then the mixing polysaccharide class aqueous solution in above-mentioned milk-acid bacteria concentrated aqueous solution, then in stirrer with 5, the speed of 000rpm stirs, carry out the process that homogenizes, thus produce the milk-acid bacteria concentrated aqueous solution using edible agar jelly, protein, polyose composition double application.
Then, implement lyophilize operation to the milk-acid bacteria concentrated aqueous solution of double application, particularly, by carrying out homogenizing, the double application milk-acid bacteria concentrated aqueous solution processed after IQF, has carried out lyophilize in the refrigerator of-55 DEG C at 0 DEG C ~ 40 DEG C.
Embodiment 2 protein and polyose that comprise edible agar carry out the preparation of the milk-acid bacteria of double application
First, using edible agar and protein to carry out bifidobacterium lactis (BifidobacteriumlactisCBT-BL) milk-acid bacteria be coated with to produce, having carried out following experiment.
Particularly, add 5% glucose as sugared composition, 3% yeast extract, 2% soybean protein isolate is as protein ingredient, 0.3% edible agar, 0.2% potassium primary phosphate, 0.2% ammonium citrate, 0.2% sodium-acetate, 0.01% magnesium sulfate, as ion component, then make them dissolve, and transfer in the anaerobic fermentation pipe of 200L capacity, sterilizing in 15 minutes is carried out at 121 DEG C, inoculation 2L bacterial classification, maintains pH with ammonia, ferments 15 hours.After above-mentioned fermentation, with the flow velocity of 60L/hr, fermented liquid is transferred in continuous centrifugal separator, make thalline and edible agar jelly precipitation, thus define milk-acid bacteria concentrated aqueous solution.In above-mentioned milk-acid bacteria concentrated aqueous solution, add 3% soybean protein isolate as protein component, thus generate bifidobacterium lactis (BifidobacteriumlactisCBT-BL) milk-acid bacteria with edible agar and protein component coating.
Then, the cryodesiccated cryoprotectant of milk-acid bacteria will be used for join in above-mentioned milk-acid bacteria concentrated aqueous solution and mix.Particularly; the cryoprotectant aqueous solution be made up of the trehalose of 30%, the sorbyl alcohol of 20% is carried out pressurization sterilizing, in above-mentioned obtained milk-acid bacteria concentrated aqueous solution, adds cryoprotectant, with 5 in stirrer; the speed of 000rpm stirs, and carries out the process that homogenizes.
Then, in order to produce bifidobacterium lactis (BifidobacteriumlactisCBT-BL) milk-acid bacteria carrying out double application with edible agar jelly, protein and polyose, following experiment has been carried out further.Pressurization sterilizing is implemented to the 3L polyose aqueous solution that mixing 1% xanthan gum, 1% Xylo-Mucine (CMC), 10% sorbyl alcohol dissolve, thus the obtained polyose aqueous solution, then in above-mentioned milk-acid bacteria concentrated aqueous solution, the above-mentioned obtained polyose aqueous solution is mixed, then in stirrer with 5, the speed of 000rpm stirs, carry out the process that homogenizes, thus produce the milk-acid bacteria concentrated aqueous solution using edible agar jelly, protein, polyose composition double application.
Then, lyophilize operation is implemented to the double application milk-acid bacteria concentrated aqueous solution comprising edible agar, particularly, the double application milk-acid bacteria concentrated aqueous solution processed homogenizing after IQF, has carried out lyophilize in the refrigerator of-55 DEG C at 0 DEG C ~ 40 DEG C.
Experimental example 1 utilizes the acid resistance of the double application lactobacillus powder of edible agar to compare
Measure after utilization obtained in above-described embodiment 1 and embodiment 2 eats the acid resistance of double application lactobacillus powder of agar, its structure is shown in Table 1.
Lactobacillus powder obtained in above-described embodiment 1 and embodiment 2 is reacted 30,60,90 and 120 minutes respectively in the artificial gastric juice of pH2.1, use physiological saline or phosphate buffer solution (Phosphate-buffer) dilution water, after being diluted by decadic dilution method, be applied on solid medium (agarplate), milk-acid bacteria is counted.
Table 1
Each check plot shows respectively and is prepared according to the method identical with embodiment 1 and embodiment 2 in table 1 above, forms the lactobacillus powder of the double application of protein and polyose under the state omitting edible agar.
As shown in above-mentioned table 1, can confirm that the embodiment 1 of preparation in accordance with the present invention and the milk-acid bacteria of embodiment 2 show higher acid resistance compared with check plot.This confirms that the acid resistance of the milk-acid bacteria of embodiment 1 and embodiment 2 is significantly higher than is not preparation in accordance with the present invention and obtained according to district.Can be confirmed by this result, the survival rate of milk-acid bacteria under hydrochloric acid in gastric juice of the embodiment 1 and embodiment 2 that contain the protein of edible agar and the double application milk-acid bacteria of polyose is significantly higher than and is not preparation in accordance with the present invention and obtained check plot.
Experimental example 2 utilizes the bile-tolerance of the double application lactobacillus powder of edible agar to compare
Measure after utilization obtained in above-described embodiment 1 and embodiment 2 eats the bile-tolerance of double application lactobacillus powder of agar, its structure is shown in Table 2.
Particularly, the double application lactobacillus powder that utilization obtained in above-described embodiment 1 and embodiment 2 eats agar is reacted 30,60,90 and 120 minutes respectively in the oxgall (oxgall) of 0.5%, use normal saline solution or phosphoric acid buffer (Phosphate-buffer) dilution water, after being diluted by decadic dilution method, be applied on solid medium (agarplate), milk-acid bacteria is counted.
Table 2
In above-mentioned table 2, each check plot shows respectively and is prepared according to the method identical with embodiment 1 and embodiment 2, forms the lactobacillus powder of the double application of protein and polyose under the state omitting edible agar.
As shown in table 2, can confirm that the embodiment 1 of preparation in accordance with the present invention and the milk-acid bacteria of embodiment 2 show higher acid resistance (bile-tolerance) compared with check plot.This confirms that the bile-tolerance of the milk-acid bacteria of embodiment 1 and embodiment 2 is significantly higher than and be not preparation in accordance with the present invention and obtained check plot.Can be confirmed by this result, the survival rate of milk-acid bacteria under bile of the embodiment 1 and embodiment 2 that contain the protein of edible agar and the double application milk-acid bacteria of polyose is significantly higher than and is not preparation in accordance with the present invention and obtained check plot.
Experimental example 3 utilizes the accelerated stability of the double application lactobacillus powder of edible agar to compare
Measure after utilization obtained in above-described embodiment 1 and embodiment 2 eats the accelerated stability of double application lactobacillus powder of agar, the results are shown in table 3.
Table 3
In above-mentioned table 3, each check plot shows respectively and is prepared according to the method identical with embodiment 1 and embodiment 2, forms the lactobacillus powder of the double application of protein and polyose under the state omitting edible agar.
As shown in above-mentioned table 3, can confirm that milk-acid bacteria accelerated test stability compared with check plot of the embodiment 1 of preparation in accordance with the present invention and embodiment 2 is higher.Can confirm that especially elapsed time longer relative stability is higher.This confirms that the milk-acid bacteria of embodiment 1 and embodiment 2 is significantly higher than for the stability of high temperature and high humidity is not such check plot.Can be confirmed by this result, containing that the embodiment 1 of the protein of edible agar and the double application milk-acid bacteria of polyose and the milk-acid bacteria of embodiment 2 be significantly higher than for the stability of high temperature and high humidity is not such check plot.
Claims (9)
1. utilize a double application lactobacillus powder for edible agar, it is characterized in that, it comprises: coating layer, comprises edible agar, protein, polyose composition form bacterium block;
Milk-acid bacteria, is coated with by described coating layer;
The double application lactobacillus powder that described utilization eats agar is obtained by the preparation method comprised the following steps:
A () adds edible agar in the substratum for cultivating milk-acid bacteria, then ferment, and forms milk-acid bacteria concentrated aqueous solution;
B () adds protein, cryoprotectant and polyose composition successively in above-mentioned milk-acid bacteria concentrated aqueous solution;
(c) to above-mentioned add edible agar, protein, polyose composition milk-acid bacteria concentrated aqueous solution carry out lyophilize;
Described step (a) is fermentation lactic acid bacteria in the aqueous solution of the protein ingredient of the sugared composition of the edible agar comprising 0.1% ~ 5.0% scope, 0.1% ~ 10% scope, the yeast extract of 0.1% ~ 10% scope, the ion component of 0.01% ~ 2.0% scope and 0.01% ~ 20% scope;
Described edible agar coating layer forms film morphology.
2. utilization according to claim 1 eats the double application lactobacillus powder of agar, it is characterized in that, described milk-acid bacteria is be selected from more than one the bacterial strain in streptococcus, lactococcus, enterococcus spp, lactobacillus, Pediococcus, Leuconostoc, Wei Si Bordetella and genus bifidobacterium.
3. utilization according to claim 1 eats the double application lactobacillus powder of agar, it is characterized in that, the main component of described edible agar is made up of agarose and agaropectin, be mixed into pH to boil in solution between 5 ~ 7, then cool and be formed as gluey, during lyophilize, the moisture of described edible agar jelly inside is removed, and forms film.
4. utilization according to claim 1 eats the double application lactobacillus powder of agar, it is characterized in that, described protein component is selected from a kind of in rice protein, soybean protein isolate, soy peptone, casein peptone, skim-milk, whey-protein or their mixture.
5. utilization according to claim 1 eats the double application lactobacillus powder of agar, it is characterized in that, described polyose composition is selected from carrageenin, xanthan gum, gelling gum, guar gum, Curdlan, Sudan Gum-arabic, algin, POLY-karaya, tragacanth gum, India(n) gum, Viscogum BE, tamarind seed gum, plantasan, pulullan polysaccharide, Wei Lan glue, sandlwood glue, pectin, Xylo-Mucine, Polylevulosan, sorbyl alcohol, Xylitol, inositol, erythritol, methylcellulose gum, Star Dri 5, glucomannan, a kind of in xylogen or their mixture.
6. utilize a preparation method for the double application lactobacillus powder of edible agar, it is characterized in that, it comprises the following steps:
A () be fermentation lactic acid bacteria in the aqueous solution of protein ingredient comprising the edible agar of 0.1% ~ 5.0% scope, the sugared composition of 0.1% ~ 10% scope, the yeast extract of 0.1% ~ 10% scope, the ion component of 0.01% ~ 2.0% scope and 0.01% ~ 20% scope, thus form milk-acid bacteria concentrated aqueous solution;
B () adds protein, cryoprotectant and polyose composition successively in above-mentioned milk-acid bacteria concentrated aqueous solution;
(c) to above-mentioned add edible agar, protein, polyose composition milk-acid bacteria concentrated aqueous solution carry out lyophilize, thus prepare the milk-acid bacteria that is coated with by coating layer, described coating layer comprises edible agar, protein, polyose composition form bacterium block; Described edible agar coating layer forms film morphology.
7. utilization according to claim 6 eats the preparation method of the double application lactobacillus powder of agar, and it is characterized in that, described step (a) comprises the following steps:
To the aqueous solution containing edible agar, sugared composition, yeast extract, ion component and protein ingredient, carry out sterilizing with the temperature of 120 DEG C ~ 130 DEG C of scopes, then cool, thus form edible agar jelly;
Above-mentioned edible agar jelly is fermented together with milk-acid bacteria, forms streptococcus acidi lactici fermented solution;
Implement to be separated and concentrated operation to above-mentioned streptococcus acidi lactici fermented solution, thus form the milk-acid bacteria concentrated aqueous solution comprising edible agar jelly.
8. the utilization comprised in claim 1 eats the product of the double application lactobacillus powder of agar.
9. product according to claim 8, is characterized in that, described product is selected from fermented-milk, modified milk, sauce class leavened food, pickle fermentation food, functional foodstuff, pharmaceuticals and makeup.
Priority Applications (1)
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| KR101764412B1 (en) * | 2015-09-14 | 2017-08-14 | 일동바이오사이언스(주) | Functionally hydrated hyaluronic acid and method of preparing coating lactic acid bacteria improving mucosal adhesive capacity and having selective antagonism in microflora |
| WO2017061628A1 (en) * | 2015-10-08 | 2017-04-13 | 三栄源エフ・エフ・アイ株式会社 | Food-improving agent |
| JP6820340B2 (en) * | 2015-12-17 | 2021-01-27 | シージェイ チェルジェダン コーポレイション | Coating method of lactic acid bacteria with enhanced intestinal survival rate |
| CN107467184A (en) * | 2017-07-24 | 2017-12-15 | 哈尔滨工业大学 | A kind of sour newborn clinker of probiotic fruit eaten suitable for children and preparation method thereof |
| CN108865922A (en) * | 2018-05-02 | 2018-11-23 | 山东出入境检验检疫局检验检疫技术中心 | listeria monocytogenes standard sample and preparation method thereof |
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| EP1514553B1 (en) * | 2003-09-05 | 2008-02-27 | Chung, Myung-jun | Lactic acid bacteria powder double-coated using protein and polysaccharide and method preparing the same and a dosage form thereof |
| WO2009093785A1 (en) * | 2008-01-25 | 2009-07-30 | Cell Biotech Co., Ltd. | Method of preparing triple-coating lactic acid bacteria and nano particle coating method, triple-coating lactic acid bacteria prepared thereby and article comprising the same |
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| WO2009093785A1 (en) * | 2008-01-25 | 2009-07-30 | Cell Biotech Co., Ltd. | Method of preparing triple-coating lactic acid bacteria and nano particle coating method, triple-coating lactic acid bacteria prepared thereby and article comprising the same |
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