CN107549309B - Fruity selenium-rich cordyceps militaris yoghourt product and preparation method thereof - Google Patents
Fruity selenium-rich cordyceps militaris yoghourt product and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a fruity selenium-rich cordyceps militaris yoghourt product and a preparation method thereof, wherein cordyceps militaris protoplast is prepared, the cell wall permeability of cordyceps militaris strains is changed, and simultaneously selenium-rich culture is carried out, so that the selenium-rich capability of cordyceps militaris can be remarkably improved, then low-fat low-sugar defatted fresh milk and selenium-rich cordyceps militaris fermentation mycelium homogenate are taken as raw materials, and hawthorn powder, pollen pini, vitamin C and the like are added to carry out mixed fermentation yoghourt, so that the low-fat low-sugar selenium-rich cordyceps militaris yoghourt with the function of reducing blood fat is prepared, the problems that fresh yoghourt has a single function, is not beneficial to storage, cannot be eaten by diabetics and the like are solved, the blood fat-reducing health-care function of the yoghourt is enhanced, and the yoghourt has a good development prospect as a.
Description
Technical Field
The invention discloses a fruity selenium-rich cordyceps militaris yoghourt product and a preparation method thereof, wherein a selenium-rich cordyceps militaris mycelium with obviously enhanced selenium enrichment capability due to cell wall permeability change and defatted fresh milk are used as main raw materials, and a plurality of auxiliary materials such as hawthorn powder, pollen pini solution, sweetening agent, vitamins and the like are added, and four mixed preparations of streptococcus thermophilus, lactobacillus bulgaricus, lactococcus lactis diacetyl subsp, and lactobacillus acidophilus are inoculated for fermentation to obtain solid yoghourt, and the fruity selenium-rich cordyceps militaris yoghourt product belongs to the technical field of fermented yoghourt.
Background
The yoghourt is prepared by fermenting milk, and has more excellent nutritive value and absorption efficiency than raw milk. Many studies have shown that yogurt has outstanding effects on various health care and nutrition functions such as resistance to cancers caused by diet and stress, and increase of body fluid production. With the rapid development of fermentation technology, in order to increase the flavor and taste of the yogurt and fully utilize the nutrient components in the fermentation medium, the fermentation process is more sufficient, and the mixed fermentation is generally adopted at present, so that the requirements of different consumers can be better met. Although the yoghourt has high nutritive value, about 7 percent of white granulated sugar is usually added, and the fat content is also very high, so the yoghourt can not be eaten by diabetics.
Selenium is a trace element necessary for human bodies, is called as a 'life miraculous element' in the trace elements, and has two states of inorganic selenium and organic selenium in nature, and the organic selenium generally exists in the form of selenomethionine, is metabolized along a methionine metabolic pathway, participates in protein synthesis, and is easy to store and absorb in tissues; can be rapidly utilized by human body after being absorbed by human body, and effectively improve the blood selenium condition in human body. The average content of selenium in adult human body is 0.25 mg/kg. The selenium intake of human body should not be less than 50 μ g/day. The recent research results of American scientists suggest that healthy people can prevent the generation of tumors to a certain extent by supplementing 200 mug/day of selenium or adding a little more selenium in a proper amount. However, the maximum safe intake of selenium is 400 μ g/day, and excessive selenium may cause poisoning. In addition, the medicine also has the functions of regulating immunity, resisting HIV and other viruses, protecting brain and enhancing male reproductive function, and is closely related to thyroid function, autoimmune thyroid diseases, septicemia and other critical diseases, cardiovascular diseases and diabetes. However, the selenium content in natural food is generally low, and the food only needs to take selenium to maintain lifeExercise has been far from satisfying the needs of health. However, excessive intake of selenium can produce acute and chronic toxic effects on the body, and simultaneously produce various toxic and side effects such as genetic toxic effect of selenium (sodium selenite), and microorganisms are utilized to treat inorganic substance sodium selenite (Na)2SeO3) The organic selenium product can be directly obtained by biotransformation, and the subsequent treatment pressure is reduced. Can improve the physiological activity and absorption rate of selenium, and reduce the toxicity of selenium.
Disclosure of Invention
The invention discloses low-fat low-sugar selenium-rich cordyceps militaris yoghourt with a blood fat reducing function, which can exert the effects of reducing blood fat, regulating immunity and the like of selenium-rich cordyceps militaris, hawthorn powder and pine pollen, has the characteristics of low sugar and low fat, has good mouthfeel, can be drunk by diabetics, is easy to store and transport, has the functions of promoting gastrointestinal digestion and the like of probiotics and the like, and has a good development prospect as a health food.
The invention relates to a preparation method of selenium-rich cordyceps militaris, which comprises the following steps:
(1) preparing cordyceps militaris protoplasts: mass of mycelium (g): adding a sterile mixed enzyme solution into the enzyme solution with the volume (mL) =1:1, and placing the enzyme solution in a shaking table at 26 ℃ for enzymolysis for 2.5 hours; centrifuging the enzymolysis solution at 6000 r/min for 12 min, discarding supernatant, cleaning precipitate with permeation stabilizer for 3 times, centrifuging to completely remove residual enzyme solution, adding permeation stabilizer, oscillating, and centrifuging at 2500 r/min for 12 min; taking the supernatant, centrifuging for 12 min at 6000 r/min, depositing the protoplast at the bottom of a centrifugal tube, and adding 2-10 parts of a permeation stabilizer for oscillating and suspending;
(2) culturing the selenium-rich cordyceps militaris: adding Na into the prepared Cordyceps militaris protoplast2SeO3 Standing at 26 deg.C for 8 hr at 60 μ g/L, transferring into 100mL Cordyceps militaris regeneration medium at 5%, and culturing at 26 deg.C under shaking (150 r/min) for 4 d;
(3) preparation of selenium-rich cordyceps militaris mycelium: centrifuging at 4000 r/min for 10 min to obtain mycelium;
(4) preparation of selenium-rich cordyceps militaris homogenate: adding 2 times volume of distilled water into 100g of mycelia, fully homogenizing by using a colloid mill, boiling for 1-5 min for inactivation to obtain selenium-enriched cordyceps militaris fermentation homogenate;
(5) the preparation of the selenium-rich cordyceps militaris yoghurt comprises the following steps: the yogurt fermentation substrate comprises: 1-10 parts of selenium-rich cordyceps militaris fermentation homogenate, 20-40 parts of fresh milk, 5-20 parts of skimmed milk powder, 0.2-0.5 part of pine pollen and 0.5 part of hawthorn powder, uniformly stirring, preserving heat at 90-95 ℃ for 10 min for sterilization, cooling to 40 ℃, inoculating 5-10 parts of a leavening agent and 0.01 part of vitamin C, culturing and fermenting at constant temperature for 24 h, stopping fermentation when the acidity is 60-80 DEG T and the solidification is complete, taking out and placing at 4 ℃ to finally obtain a finished yoghourt product.
The pine pollen is fresh yellow or light yellow fine powder, is sweet and non-toxic, is a traditional Chinese food, is rich in fiber and low in calorie, contains various balanced nutrients, contains fatty acid and vitamins with synergistic effect, and can regulate cholesterol and blood fat of human body, so that the pine pollen has the effect of reducing blood fat. The hawthorn is also a traditional health food used as both medicine and food, and has the effects of reducing blood fat, strengthening the spleen and stomach and the like.
In the culture process of the selenium-rich cordyceps militaris, the cordyceps militaris protoplast is prepared by adopting an enzymolysis method, the cell wall permeability of cordyceps militaris spores and mycelia is changed, the capability of the cordyceps militaris for absorbing inorganic selenium can be effectively improved, and the biotransformation efficiency of the inorganic selenium is further improved. Then, the essences of the selenium-rich cordyceps militaris, the hawthorn and the pine pollen are blended into the yogurt preparation process, so that the yogurt has good health care efficacy and edible value.
The invention has the positive effects that:
according to the invention, the cordyceps militaris protoplast is prepared, the cell wall permeability of cordyceps militaris strains is changed, meanwhile, selenium-rich culture is carried out, the selenium-rich capability of cordyceps militaris can be remarkably improved, then low-fat low-sugar defatted fresh milk and selenium-rich cordyceps militaris fermentation mycelium homogenate are taken as raw materials, hawthorn powder, pine pollen, vitamin C and the like are added, mixed bacteria fermentation yoghourt is carried out, and the low-fat low-sugar selenium-rich cordyceps militaris yoghourt with the blood fat reducing function is prepared.
Detailed Description
The present invention is further illustrated by the following examples, which do not limit the present invention in any way, and any modifications or changes that can be easily made by a person skilled in the art to the present invention will fall within the scope of the claims of the present invention without departing from the technical solution of the present invention.
The embodiment of the invention relates to a strain culture medium and a reagent for fermenting selenium-rich cordyceps militaris and yoghourt, which comprise:
strain:Cordycepsmilitaris(L.ex Fr.) Link (pure strain obtained by tissue isolation from wild Cordyceps militaris and identified by institute of microbiology of Chinese academy of sciences). Streptococcus thermophilus, Lactobacillus bulgaricus, Lactobacillus lactis diacetyl subspecies, Lactobacillus acidophilus zymocyte powder, available from Danisco (China) Limited.
The cordyceps militaris strain activation culture medium comprises: PDA culture medium.
Activation of cordyceps militaris strains: selecting Cordyceps militaris stored in slant, inoculating to 100mL strain activation medium, and culturing at 26 deg.C under shaking (150 r/min) for 3 d.
Cordyceps militaris regeneration medium: 15g/L glucose, 8g/L peptone, 10g/L yeast extract, 1.5 g/L potassium dihydrogen phosphate, 1.5 g/L magnesium sulfate heptahydrate, VB10.1 g/L, ammonium sulfate 10g/L, mannitol 0.5mol/L, Na2SeO3 30. mu.g/L. Sterilizing at 115 deg.C for 30 min.
A penetration stabilizer (0.5 mol/L): 0.5mol/L mannitol was prepared using phosphate buffer pH7.2 and sterilized at 121 ℃ for 20 min.
Enzyme solution: preparing 1% cellulase and 0.5% snailase by a penetration stabilizer, mixing in equal volume, and filtering with a 0.22 μm filter membrane for sterilization.
The leavening agent comprises the following components: the lactobacillus acidophilus powder is prepared by mixing four mixed bacteria powders of streptococcus thermophilus, lactobacillus bulgaricus, lactococcus lactis diacetyl subspecies and lactobacillus acidophilus according to the ratio of 1:1:1: 1.
Example 1
(1) Preparing cordyceps militaris protoplasts: mass of mycelium (g): the volume of the enzyme solution (mL) =1:1, adding sterile mixed enzyme solution, and placing in a shaking table at 26 ℃ for enzymolysis for 2.5 h. Centrifuging the enzymolysis solution at 6000 r/min for 12 min, discarding supernatant, cleaning precipitate with permeation stabilizer for 3 times, centrifuging to completely remove residual enzyme solution, adding permeation stabilizer, oscillating, and centrifuging at 2500 r/min for 12 min. Taking supernatant, centrifuging for 12 min at 6000 r/min, depositing protoplast at the bottom of a centrifuge tube, adding 2 parts of a penetration stabilizer, and oscillating and suspending;
(2) culturing the selenium-rich cordyceps militaris: adding Na into the prepared Cordyceps militaris protoplast2SeO3 Standing at 26 deg.C for 8 hr at 60 μ g/L, transferring into 100mL Cordyceps militaris regeneration medium at 5%, and culturing at 26 deg.C under shaking (150 r/min) for 4 d;
(3) preparation of selenium-rich cordyceps militaris mycelium: centrifuging at 4000 r/min for 10 min to obtain mycelium;
(4) preparation of selenium-rich cordyceps militaris homogenate: adding 2 times volume of distilled water into 100g of mycelia, fully homogenizing by using a colloid mill, boiling for 1-5 min for inactivation to obtain selenium-enriched cordyceps militaris fermentation homogenate;
(5) preparing a yoghourt fermentation substrate: 1 kg of selenium-rich cordyceps militaris fermentation homogenate, 20 kg of fresh milk, 5kg of skimmed milk powder, 0.2 kg of pollen pini and 0.5 kg of hawthorn powder are uniformly stirred, kept at 90-95 ℃ for 10 min for sterilization and cooled to 40 ℃;
(6) fermentation of the yogurt: inoculating 5kg of a starter and 10g of vitamin C, culturing and fermenting at constant temperature for 24 h, stopping fermentation when the acidity is 60-80 DEG T and the solidification is complete, taking out, and standing at 4 ℃ to obtain the finished yoghourt.
Example 2
(1) Preparing cordyceps militaris protoplasts: mass of mycelium (g): the volume of the enzyme solution (mL) =1:1, adding sterile mixed enzyme solution, and placing in a shaking table at 26 ℃ for enzymolysis for 2.5 h. Centrifuging the enzymolysis solution at 6000 r/min for 12 min, discarding supernatant, cleaning precipitate with permeation stabilizer for 3 times, centrifuging to completely remove residual enzyme solution, adding permeation stabilizer, oscillating, and centrifuging at 2500 r/min for 12 min. Taking the supernatant, centrifuging for 12 min at 6000 r/min, depositing the protoplast at the bottom of a centrifugal tube, adding 4 parts of a penetration stabilizer, and oscillating and suspending;
(2) culturing the selenium-rich cordyceps militaris: adding Na into the prepared Cordyceps militaris protoplast2SeO3 Standing at 26 deg.C for 8 hr at 60 μ g/L, transferring into 100mL Cordyceps militaris regeneration medium at 5%, and culturing at 26 deg.C under shaking (150 r/min) for 4 d;
(3) preparation of selenium-rich cordyceps militaris mycelium: centrifuging at 4000 r/min for 10 min to obtain mycelium;
(4) preparation of selenium-rich cordyceps militaris homogenate: adding 2 times volume of distilled water into 100g of mycelia, fully homogenizing by using a colloid mill, boiling for 1-5 min for inactivation to obtain selenium-enriched cordyceps militaris fermentation homogenate;
(5) preparing a yoghourt fermentation substrate: 2kg of selenium-rich cordyceps militaris fermentation homogenate, 30kg of fresh milk, 10kg of skimmed milk powder, 0.3 kg of pollen pini and 0.5 kg of hawthorn powder are uniformly stirred, kept at 90-95 ℃ for 10 min for sterilization and cooled to 40 ℃;
(6) fermentation of the yogurt: inoculating 7 kg of a starter and 0.01 kg of vitamin C, culturing and fermenting for 24 h at a constant temperature, stopping fermentation when the acidity is 60-80 DEG T and the solidification is complete, taking out and placing at 4 ℃ to obtain the finished product of the yoghourt.
Example 3
(1) Preparing cordyceps militaris protoplasts: mass of mycelium (g): the volume of the enzyme solution (mL) =1:1, adding sterile mixed enzyme solution, and placing in a shaking table at 26 ℃ for enzymolysis for 2.5 h. Centrifuging the enzymolysis solution at 6000 r/min for 12 min, discarding supernatant, cleaning precipitate with permeation stabilizer for 3 times, centrifuging to completely remove residual enzyme solution, adding permeation stabilizer, oscillating, and centrifuging at 2500 r/min for 12 min. Taking the supernatant, centrifuging for 12 min at 6000 r/min, depositing the protoplast at the bottom of a centrifugal tube, adding 6 parts of a penetration stabilizer, and oscillating and suspending;
(2) culturing the selenium-rich cordyceps militaris: adding Na into the prepared Cordyceps militaris protoplast2SeO3 Standing at 26 deg.C for 8 hr at 60 μ g/L, transferring into 100mL Cordyceps militaris regeneration medium at 5%, and culturing at 26 deg.C under shaking (150 r/min) for 4 d;
(3) preparation of selenium-rich cordyceps militaris mycelium: centrifuging at 4000 r/min for 10 min to obtain mycelium;
(4) preparation of selenium-rich cordyceps militaris homogenate: adding 2 times volume of distilled water into 100g of mycelia, fully homogenizing by using a colloid mill, boiling for 1-5 min for inactivation to obtain selenium-enriched cordyceps militaris fermentation homogenate;
(5) preparing a yoghourt fermentation substrate: 3 kg of selenium-rich cordyceps militaris fermentation homogenate, 35 kg of fresh milk, 15 kg of skimmed milk powder, 0.4 kg of pollen pini and 0.5 kg of hawthorn powder are uniformly stirred, kept at 90-95 ℃ for 10 min for sterilization and cooled to 40 ℃;
(6) fermentation of the yogurt: inoculating 8 kg of a starter and 0.01 kg of vitamin C, culturing and fermenting for 24 h at a constant temperature, stopping fermentation when the acidity is 60-80 DEG T and the solidification is complete, taking out and placing at 4 ℃ to obtain the finished product of the yoghourt.
Example 4
(1) Preparing cordyceps militaris protoplasts: mass of mycelium (g): adding a sterile mixed enzyme solution into the enzyme solution with the volume (mL) =1:1, and placing the enzyme solution in a shaking table at 26 ℃ for enzymolysis for 2.5 hours; centrifuging the enzymolysis solution at 6000 r/min for 12 min, discarding supernatant, cleaning precipitate with permeation stabilizer for 3 times, centrifuging to completely remove residual enzyme solution, adding permeation stabilizer, oscillating, and centrifuging at 2500 r/min for 12 min. Taking the supernatant, centrifuging for 12 min at 6000 r/min, depositing the protoplast at the bottom of a centrifugal tube, adding 10 parts of a penetration stabilizer, and oscillating and suspending;
(2) culturing the selenium-rich cordyceps militaris: adding Na into the prepared Cordyceps militaris protoplast2SeO3 Standing at 26 deg.C for 8 hr at 60 μ g/L, transferring into 100mL Cordyceps militaris regeneration medium at 5%, and culturing at 26 deg.C under shaking (150 r/min) for 4 d;
(3) preparation of selenium-rich cordyceps militaris mycelium: centrifuging at 4000 r/min for 10 min to obtain mycelium;
(4) preparation of selenium-rich cordyceps militaris homogenate: adding 2 times volume of distilled water into 100g of mycelia, fully homogenizing by using a colloid mill, boiling for 1-5 min for inactivation to obtain selenium-enriched cordyceps militaris fermentation homogenate;
(5) preparing a yoghourt fermentation substrate: 10kg of selenium-rich cordyceps militaris fermentation homogenate, 40 kg of fresh milk, 20 kg of skimmed milk powder, 0.5 kg of pollen pini and 0.5 kg of hawthorn powder are uniformly stirred, kept at 90-95 ℃ for 10 min for sterilization and cooled to 40 ℃;
(6) fermentation of the yogurt: inoculating 10kg of a starter and 0.01 kg of vitamin C, culturing and fermenting for 24 h at a constant temperature, stopping fermentation when the acidity is 60-80T and the solidification is complete, taking out and placing at 4 ℃ to obtain the finished product of the yoghourt.
The sensory qualities of yoghurts prepared in examples 1 to 4 of the present invention are shown in table 1:
table 1:
test example 1
Research on anti-fatigue activity of selenium-enriched cordyceps sinensis fermented yoghourt
Healthy male mice were taken, acclimated for 5 days before the experiment, and randomly divided into 6 groups: in the yogurt examples 1-4 groups, the negative control group (the yogurt group without selenium-enriched cordyceps) and the blank control group (0.1 ml/10g of normal saline) were stirred uniformly with 0.2ml of water, and then the mixture was continuously perfused into the stomach for 25 days, and free diet and drinking water were provided during the modeling period. After modeling, randomly selecting 10 mice in each group, after feeding yoghourt for 30min at the last time, loading lead blocks with the weight of 5% on the heel parts of the mice, placing the mice in a plastic bucket with the water depth of 30 cm, the diameter of 15 cm and the water temperature of 27-30 ℃, recording the swimming time of the mice, and taking the condition that the heads of the mice are completely immersed in the water and can not float out of the water again for 8s as a swimming finish mark. Removing blood from eyeball, standing at 37 deg.C for 30min to separate out serum. Centrifuging (1500 r/min) for 5min, collecting supernatant, and measuring SOD and MDA values and lactic acid content of blood serum; killing a mouse by dislocation of cervical vertebra, dissecting and taking liver, cleaning with normal saline, accurately weighing a certain amount of liver tissue after surface moisture is absorbed by filter paper, adding normal saline, homogenating in ice bath, centrifuging for 15 min at 3000 r/min by a low-temperature centrifuge, and taking supernatant to measure liver glycogen content. The results are shown in tables 5 to 6:
table 5 effect of yoghurt of the invention on weight bearing swimming time of mice (N = 10)
P <0.01, P <0.001 compared to control.
Table 6 effect of yoghurt of the invention on the viability of biochemical markers in mice (N = 10)
P <0.05 compared to control group.
As can be seen from Table 5, the weight-bearing swimming time of the mice can be remarkably increased after the yogurt is fed, and the anti-fatigue activity of the yogurt is superior to that of the negative control group.
Table 6 shows that, compared with the blank control group, the mouse eating the yogurt has the advantages of increased serum SOD activity, reduced serum MDA content, reduced blood lactic acid content, and significantly increased liver glycogen content, and thus, the anti-fatigue activity of the mouse can be improved by using the selenium-rich cordyceps militaris yogurt for a long time.
Test example 2
The invention has the function of reducing blood fat of mice with hyperlipidemia caused by intraperitoneal injection of Triton
90 normal mice with half male and female are taken, and after being fed normally for 3d, the mice are randomly divided into 9 groups, namely a normal group, a hyperlipemia model group, a simvastatin group (6 mg/kg), a negative control group (a selenium-free cordyceps sinensis yoghourt group) and yoghourt examples 1-4. Except for the normal group of mice, other 6 groups of mice are respectively modeled by injecting 4% Triton solution into the abdominal cavity with the volume of 10 ml/kg, and after injection, the control group and the model group are given with the same volume of normal saline, and other groups are continuously injected with gastric yoghurt for 25 days. 3 h after the last time of feeding yoghourt, the mouse picks eyes to take blood, serum is separated, and TC, TG and HDL-C, LDL-C are respectively measured according to the instruction of the kit.
TABLE 7 hypolipidemic Effect of the yoghurt of the invention on mice with hyperlipidemia induced by intraperitoneal injection of Triton (mean. + -. SD; n = 10)
P <0.05, P <0.01, compared to control group, # P <0.05, compared to model group.
Test example 3
The yoghourt has the function of reducing blood fat of baiting hyperlipidemic rats
80 normal male SD rats were randomly divided into 8 groups of 10 rats. The control group was fed with normal feed, the remaining 6 groups were fed with high-fat feed (2% cholesterol, 0.5% bovine bile salt, 10% lard, 0.1% propylthiouracil, 87.3% basal feed), and simultaneously simvastatin (6 mg/kg) was administered by intragastric administration in a volume of 10 ml/kg, the negative control group (non-selenium-enriched cordyceps yogurt group) or yogurt examples 1-4 groups, the normal group and the high-fat model group were administered with the same amount of physiological saline for 28 days continuously, blood was taken from the eyeball after the last feeding, and serum was separated to determine TC, TG, HDL-C, LDL-C.
TABLE 8 hypolipidemic Effect of the yoghurt of the invention on baited hyperlipidemic rats (mean. + -. SD; n = 10)
P <0.05 compared to control group, # P <0.01 compared to model group, # P < 0.05.
Claims (1)
1. A preparation method of fruity selenium-rich cordyceps militaris yoghourt product comprises the following steps:
1) preparing cordyceps militaris protoplasts: according to the mass g of mycelium: adding a sterile mixed enzyme solution into the enzyme solution with the volume mL =1:1, and placing the enzyme solution in a shaking table at the temperature of 24-28 ℃ for enzymolysis for 2.0-3.0 h; centrifuging the enzymolysis solution at 6000 r/min for 12 min, discarding supernatant, cleaning precipitate with permeation stabilizer for 3 times, centrifuging to completely remove residual enzyme solution, adding permeation stabilizer, oscillating, and centrifuging at 2500 r/min for 12 min; taking the supernatant, centrifuging for 12 min at 6000 r/min, depositing the protoplast at the bottom of a centrifugal tube, and adding 2-10 parts of a permeation stabilizer for oscillating and suspending;
2) culturing the selenium-rich cordyceps militaris: adding 60 μ g/L inorganic selenium sodium selenite into the prepared Cordyceps militaris protoplast, standing at 26 deg.C for 8 hr, transferring into 100mL Cordyceps militaris regeneration medium at 5% ratio, and culturing at 26 deg.C under shaking for 150 r/min for 4 d;
3) preparation of selenium-rich cordyceps militaris mycelium: centrifuging at 4000 r/min for 10 min to obtain mycelium; cordyceps militaris regeneration medium: 15g/L glucose, 8g/L peptone, 10g/L yeast extract, 1.5 g/L potassium dihydrogen phosphate, 1.5 g/L magnesium sulfate heptahydrate, VB1 0.1 g/L of ammonium sulfate, 10g/L of ammonium sulfate, 0.5mol/L of mannitol and 30 mu g/L of sodium selenite; sterilizing at 115 deg.C for 30 min;
4) adding distilled water with 2 times of volume into 100g of mycelia prepared in the step 3), fully homogenizing by using a colloid mill, boiling for 1-5 min for inactivation, and preparing selenium-rich cordyceps militaris fermentation homogenate;
5) preparing a yoghourt fermentation substrate:
1 to 10 portions of selenium-rich cordyceps militaris fermentation homogenate, 20 to 40 portions of fresh milk, 5 to 20 portions of skimmed milk powder, 0.2 to 0.5 portion of pine pollen and 0.5 portion of hawthorn powder are evenly stirred, the temperature is kept at 90 to 95 ℃ for 10 min for sterilization, and the mixture is cooled to 40 DEG C
6) Fermentation of the yogurt: inoculating 5-10 parts of a leavening agent and 0.01 part of vitamin C, culturing and fermenting for 24 hours at constant temperature, stopping fermentation when the acidity is 60-80 DEG T and the solidification is complete, taking out and placing at 4 ℃ to obtain the microbial fertilizer;
the leavening agent is: the lactobacillus acidophilus powder is prepared by mixing four mixed bacteria powders of streptococcus thermophilus, lactobacillus bulgaricus, lactococcus lactis diacetyl subspecies and lactobacillus acidophilus according to the ratio of 1:1:1: 1.
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| CN111631327A (en) * | 2019-03-01 | 2020-09-08 | 张德珍 | Multi-vitamin beverage containing nano-selenium |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1266623A (en) * | 2000-03-23 | 2000-09-20 | 周国君 | Cordyceps milk and preparing process thereof |
| CN101422196A (en) * | 2008-10-31 | 2009-05-06 | 上海双金生物科技有限公司 | Functional yoghourt and production method thereof |
| CN102578230A (en) * | 2012-02-21 | 2012-07-18 | 常熟市方塔涂料化工有限公司 | Health-care yoghourt and preparation method thereof |
| CN106119121A (en) * | 2016-06-24 | 2016-11-16 | 河北科技大学 | Selenium-rich cordyceps bacterium and cultural method, selenium-enriched yellow wine and preparation method thereof |
| CN106509142A (en) * | 2016-10-26 | 2017-03-22 | 翔天农业开发集团股份有限公司 | Mycelium fluid health-care product and preparation method thereof |
-
2017
- 2017-09-13 CN CN201710820132.2A patent/CN107549309B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1266623A (en) * | 2000-03-23 | 2000-09-20 | 周国君 | Cordyceps milk and preparing process thereof |
| CN101422196A (en) * | 2008-10-31 | 2009-05-06 | 上海双金生物科技有限公司 | Functional yoghourt and production method thereof |
| CN102578230A (en) * | 2012-02-21 | 2012-07-18 | 常熟市方塔涂料化工有限公司 | Health-care yoghourt and preparation method thereof |
| CN106119121A (en) * | 2016-06-24 | 2016-11-16 | 河北科技大学 | Selenium-rich cordyceps bacterium and cultural method, selenium-enriched yellow wine and preparation method thereof |
| CN106509142A (en) * | 2016-10-26 | 2017-03-22 | 翔天农业开发集团股份有限公司 | Mycelium fluid health-care product and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 秦巴蛹虫草原生质体的制备及再生条件研究;马跃腾等;《西北农林科技大学学报（自然科学版）》;20150930;第43卷(第9期);第196-202、209页 * |
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