CN105385612B - Candida utili bacterium, its composition and application - Google Patents
Candida utili bacterium, its composition and application Download PDFInfo
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- CN105385612B CN105385612B CN201510952295.7A CN201510952295A CN105385612B CN 105385612 B CN105385612 B CN 105385612B CN 201510952295 A CN201510952295 A CN 201510952295A CN 105385612 B CN105385612 B CN 105385612B
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- candida utili
- fermentation
- probiotics
- cotton dregs
- gossypol
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/165—Yeast isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/72—Candida
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
Abstract
The present invention provides one plant of new candida utili bacterium, the application of the probiotics composition and candida utili bacterium containing the bacterium and its probiotics composition in cotton dregs fermentation and liquid waste processing containing ammonia nitrogen.The present invention also provides the methods for preparing fermented feed probiotics composition.The present invention also provides the methods for obtaining high ammonia nitrogen assimilability and Cambodia's Phenol degradation rate saccharomycete mutant strain by ultraviolet mutagenesis breeding screening.Candida utili bacterium ammonia nitrogen assimilability provided by the invention is high, and degradation gossypol ability is strong, and fermentation thalli biomass and protein content are high.Ammonia-nitrogen content can effectively be reduced using bacterial strain processing waste liquid containing ammonia nitrogen, and feeding mycoprotein can be formed；Using probiotics containing the bacterial strain and combinations thereof fermentation cotton dregs, the content of Toxic gossypol in cotton dregs can be significantly reduced, improve crude protein content and water-soluble protein content, and can partial alternative dregs of beans for raising animal.
Description
Field that the present invention belongs to：
The invention belongs to biological feedstuff and biological environmental production fields, specifically, the present invention relates to candida utili bacterium, containing
Application of the fermenting agent composition and the saccharomycete and its composition of the bacterium in feed fermentation and liquid waste processing.
Background technology：
It is that can be used as the safety life of food additives that candida utili (Candida utilis), which is through U.S. FDA certification,
Object (GRAS) can be used as safe food in China or activated feed additive use.Candida utili can produce gluathione
(Wei Gongyuan waits process engineering journals, 2005,5 (3) to peptide：327-331), the flavor substances such as yeast extract (it is full of leaves, wait to eat
Product industrial technology, 2014,25 (33)：The substances such as 223-225), are widely used in food and pharmacy industry.Candida utili is rich in egg
White matter and B family vitamin are also act as livestock, poultry forage protein.
The ability of conversion of saccharomycetes nonprotein nitrogen (such as urea or ammonium salt) is the important of embodiment saccharomycete biotransformation capacity
Index, the ability that different strains convert nonprotein nitrogen are different.It is organic nitrogen that candida utili, which has stronger conversion inorganic nitrogen,
Ability, can be using urea and ammonium nitrogen as nitrogen source, additionally it is possible to using various wastes as raw material produce edible protein, such as
Wheat bran, molasses, potato starch wastewater (Ge1inas et al., Bioresour Technology, 2007,98 (5)：
1138-1143), (Gao Weiwei waits biotechnologys, 2008 18 (5) to a variety of organic acids such as lactic acid：58-60) etc., it is raised in fermentation
The fields such as material and wastewater treatment all have the foreground of being widely applied.(Food Science, 2009,30 (21) such as He Dongdong：210-213)
By the ultraviolet mutagenesis to candida utili bacterium, screening obtains the mutagenic strain that protein content is 33%.Chen Jia is waited and (is raised
Material industry, 2013,34 (5)：53-55) using candida utili as starting strain, gourmet powder waste water, corn flour and wheat bran are for experiment
Substrate, solid state fermentation produce fodder yeast, the results showed that：Gourmet powder waste water can be used for producing protein as cheap nonprotein nitrogen raw material
In the production of Candida solid state fermentation, there is good economic benefit and potentiality to be exploited.
Cotton dregs are the second largest plant protein resources that China is only second to Soybean Meal, but due to containing main noxious material in cotton dregs
Gossypol can generate serious harmful effect to animal growth and breeding etc., also influence human health indirectly, greatly limit
Large-scale application of the cotton cake dregs on feed industry is made.Gossypol poison-removing method mainly have physics detoxicity method, chemical passivation method,
Extraction method and biological detoxication method etc..Utilized microbial fermentation cotton dregs to reduce the detoxification of wherein free gossypol content in recent years
Method is increasingly taken seriously, and mainly can also improve cotton while reducing free gossypol content due to microbe fermentation method
The nutritive value of the seedcake dregs of rice, such as improve protein quality and digestibility (Xiaxin at equal composite fermentations Nutritional Value of Cottonseed Meal and
Activated product is analyzed and researched, Chinese grain and oil journal, 2012,25 (2)：96-100)；The Cottonseed Meals such as Nie Cunxi source fermentation protein
The metabolite of feed is studied, Animal nutrition journal, 2012,24 (8):1602-1609).
Report at present with cotton dregs for main fermentation substrate, the microorganism of degradable gossypol includes mainly saccharomycete, mould
With bacterium etc..The microorganism of different researcher's reports is widely different to the removal rate of gossypol in cotton dregs, highest up to 90%
(such as Wu little Yue utilize the research of Microbes To Break Down Free-gossypol In Cottonseed Meal, Scientia Agricultura Sinica, 1989,22 (2) above：
82-86；The mutagenic and breeding Cottonseed Meal high-efficiency detoxicating bacterial strains such as vast stretch of wooded country crystalline substance, journal of Zhejiang university (agricultural and life science version),
2011,37(3):252-258), the low (sieve of free gossypol strain in the efficient degradation cotton dregs such as Liu Jiancheng that is only 50% or so
Choosing, feed industry, 2012,33 (1)：29-31；The Enterococcus faecalis fermentations such as horse treasure give birth to the optimization of process conditions of cotton dregs, food and hair
Ferment industry, 2013,39 (5):94-98).Whether this is mainly handled by high-temperature sterilization with cotton dregs raw material before fermentation and the hair of use
Yeast-like fungi kind and combinations thereof is different related.High-temperature sterilization itself can make free gossypol and protein and amino acid etc. in conjunction with and formed knot
Gossypol is closed, free gossypol content is made to be remarkably decreased.The effect of different microorganisms strain detoxification have notable difference (Yang Xia, Weng Xiaoyan,
Separation, the screening and identification of the gossypol degradation bacteria strains such as Guo Jianlin, Cotton Science, 2010, (6)：539-546).It is superfine in grandson (micro-
Biology is notified to, 2011,38 (8):1166-1171) report the Pichi strain (Pichia of 1 high-efficiency degradation gossypol
Guilliermondii), under the conditions of without humid heat treatment, virus elimination rate reaches 58% after inoculation fermentation.(the feeds such as Cheng Fuliang
Wide-angle, 2012,14:Solid fermentation detoxification 22-23) is carried out to the cotton dregs after high temperature high pressure process using candida utili,
As a result cotton dregs are shown before and after fermentation, gossypol removal rate is 85.7% or so.(Chinese oil, 2013,38 (5) such as Qiao Xiaoyan：
Candida tropicalis JD-9 and Lactobacillus casei MX-48 fermentation cotton dregs 30-33) are utilized, reaching improves cotton dregs Forage quality
Purpose.The result shows that:The gossypol content 520mg/kg before fermentation that dissociates in cotton dregs after fermentation is reduced to 270mg/kg, virus elimination rate to reach
To 48.1%；Zhou Huilin etc. (Wuhan Polytechnic University, 2014,33 (1):14-16) by comparing different bacto yeasts with acetic acid cotton
Phenol is the upgrowth situation on the solid medium of sole carbon source, filters out one plant of well-grown production in gossypol acetate culture medium
Protein Candida 1087.It is Inoculant with candida utili 1087, is sent out for the solid of primary raw material with cotton dregs and sweet potato waste etc.
Ferment is tested, the results showed that, the sample crude protein content after fermentation improves 37.4%, and gossypol content has dropped 55.6%.
Bacillus licheniformis and lactobacillus plantarum are " the feed addictive kind mesh that the Ministry of Agriculture of China announced in 2013
The microorganism that can be added in feed in record ".Bacillus licheniformis is with its high temperature resistant, acid and alkali-resistance, resistance are strong, easy spies such as storage
It selects and is widely used as probiotics or pathogenic bacteria competitive inhibitor applied in livestock and poultry breeding industry.The bacterium, can in growth course
A variety of enzymes for helping to digest and assimilate such as extracellular proteinase, amylase and lipase are being reduced and are being eliminated in anti-nutritional factors
Also played important function (Zhang Ju, waits China feed, 2012,17：9-11).The lactic acid bacterias such as lactobacillus plantarum as people and
Normal flora in animal body has the function of to adjust enteron aisle, promote digestion etc., it is considered to be the ideal strain of probiotics is produced,
It is commonly applied to fermenting agent (the grain and feed industries such as golden stake, 2010 (3) of the food such as milk and feed：37-40).This
Two kinds of bacteriums are reported is mixed for fermentation cotton dregs with other microorganisms, but its ferment effect is not quite similar.Xu Jing etc. (in
State's grease, 2012,37 (7)：Microbe in solid state raw material fermentation 16-19) is had studied to free gossypol in Cottonseed Meal and protein
The influence of changes of contents, the results showed that, bacillus licheniformis Bl2 detoxification efficiencies are best in the experiment of single bacterium Cottonseed Meal solid state fermentation,
Virus elimination rate is 42.7%, water-soluble protein content 8.2%, is mixed in bacterium combined fermentation, bacillus licheniformis Bl2+ Candida utilis ferment
Female Cu1 is optimal combination, and virus elimination rate 50.03%, water-soluble protein content 9.11%, mixed fungus fermentation effect is better than single bacterium
Fermentation.(feed industry, 2007,28 (18) such as Zhang Qinghua：3 bacterial strains collaboration solid state fermentation 37-38) is reported to imitate cotton cake toxicity removal
The influence of fruit and its bioactivity, the results showed that, 3 bacterial strains mixed culture can significantly reduce the gossypol content that dissociates in cotton dregs substrate,
Its optimum process condition is:Candida tropicalis:Endomycopsi.sp:Lactobacillus plantarum proportioning is 3:2:1；Cotton dregs:Corn flour:Wheat bran
Than being 6:2:When 2, the removal rate of cotton dregs is 85.30%.And (hubei agricultural science, 2013,52 (15) such as Yang Yu：3596-
3597) report mixes beneficial flora in ripe hair using aspergillus niger, yeast, lactobacillus plantarum and the bacillus filtered out
Fermentation Hubei cotton dregs and rapeseed meal under the conditions of ferment, the results showed that, after fermentation process, the free gossypol in raw material contains Hubei cotton dregs
Amount increases 129.15% instead.Different strain combined fermentation handles Cottonseed Meal, and detoxification efficiency also will produce prodigious difference
(leaf is bright strong etc., Chinese feed, and 2009,15:8-14).
Chinese patent CN201010218533 discloses a kind of composite and highly effective microorganism system for refractory wastewater
Agent；Chinese patent CN200310107801 discloses a kind of microbiological ammonia nitrogen aligning agent；Chinese patent CN 201210329933
Disclosure of the invention is a kind of improve cottonseed meal content and can detoxification biological fermentation process；Chinese patent CN201110102144 is disclosed
It is fermented the method for decomposing gossypol in cotton dregs with composite bacteria；Chinese patent CN201310440055 discloses a kind of micro- life of cotton dregs
Object leavening and preparation method thereof；Chinese patent CN201410248620 is disclosed adds mixed enzyme fermentation cotton dregs to degrade with compound bacteria
The method of gossypol；Chinese patent CN200810044216 discloses a kind of method that mixed fermentation handles the feedstuffs such as vinasse；
Chinese patent CN201110161862 discloses a kind of ruminant micro-ecological additive and its preparation process.
Currently, domestic often add part of sulfuric acid ammonium or urine in the technique of exploitation unconventional protein feedstuff such as fermentation cotton dregs
The nonprotein nitrogen such as element are expected that by saccharomycetes to make fermentation and are translated into the available mycoprotein of animal, increase fermented feed
Protein content, meanwhile, the content for reducing noxious material gossypol in Cottonseed Meal is acted on by microbial fermentation.This requires fermentations
The yeast strain applied in cotton dregs production should be provided simultaneously with the energy of stronger nonprotein nitrogen conversion capability and stronger degradation gossypol
Power.Shortcoming is that function is relatively simple existing for the candida utili bacterial strain reported at present.(the Wuhan light industry such as Cao Yufei
College journal, 2015:34(2)：It 1-5) reports from 4 categories such as Candida and saccharomyces cerevisiae totally 56 plants of thermotolerant yeast bacterium
Screening obtains 1 plant of ammonia nitrogen assimilability up to 81.1% candida utili (C.utilis) SG607, but insufficient existing for the bacterial strain
It is not strong to the degradation capability of gossypol, low-level is in the assimilability of ammonia nitrogen.Have not yet to see be provided simultaneously with it is same to ammonia nitrogen
The report of change ability and the very high wild type candida utili or its mutant strain of gossypol degradation capability.
Generated water-soluble protein content is limited when single yeast strain is for protein feeds such as fermentation cotton dregs, to being promoted
The nutritive value of feed is unfavorable.Strain mixed fermentation is combined the problem is that the matching of strain is inappropriate, single in mixed bacteria
The functional not strong or mixed proportion of one strain is improper, often leads to that fermentation cotton dregs are ineffective or there are safety issues.
Inventive technique content：
It is an object of the present invention to provide a kind of new candida utili bacterial strain, assimilability of the bacterial strain to ammonia nitrogen
Height can handle the organic liquid waste containing ammonia nitrogen using the bacterial strain and produce fodder yeast mycoprotein to the good degrading effect of gossypol,
Can fermentation cotton dregs detoxification and efficiently utilize nonprotein nitrogen produce high protein feed.
It is a further object to provide a kind of probiotics composition containing candida utili.
It is also another object of the present invention to provide candida utili in the cotton dregs containing nonprotein nitrogen such as ammoniacal nitrogens that ferment
Application.
It is also another object of the present invention to provide prepare containing candida utili, bacillus licheniformis and lactobacillus plantarum
The method of probiotics composition.
It is also another object of the present invention to provide the probiotics compositions containing candida utili in cotton dregs fermentation
Application.
The invention discloses one plant of new candida utili bacterium, by through soil separation candida utili (C.utilis)
SG607 mutagenesis generates, which is characterized in that and the bacterial strain is candida utili SG60765 (Candida utilis SG60765),
It is preserved in China typical culture collection center, deposit number is CCTCC NO:2015718.Preservation date is December 3 in 2015
Day.China typical culture collection center is located at Wuhan City, Hubei Province Wuchang District Wuhan University in the school, phone：027-
68752319, EMAIL：After the present invention discloses, third party researcher can obtain from preservation mechanism cctcc@whu.edu.cn. in accordance with the law
Strain is taken to reproduce the technology contents of the present invention.
SG60765 plants of candida utili is detached from Hubei soil and is obtained through ultraviolet mutagenesis screening.The bacterial strain just like
Lower feature：
Candida utili SG60765 plant shape states are characterized in：In YPD 1~2d of cultured on solid medium, bacterium colony is milky white
Color, cheese shape, surface is smooth, relatively moistens, and cultivates 12~14h at 37 DEG C in YPD fluid nutrient mediums, cell is spherical in shape, oval
Or ellipse, size are (2.2~5.0) μ m (2.5~5.5) μm.
SG60765 plants of biochemical characters of candida utili are：It can ferment using sucrose, galactolipin, trehalose, maltose,
Xylose, lactose, cellobiose, gossypose.
SG60765 plants of candida utili assimilates ammonia nitrogen ability characteristics：At 37 DEG C, in the training of the ammonia nitrogen containing 160mg/L
It supports in base, SG60765, up to 94.2%, is saccharomyces cerevisiae CCTCC AY92003 and Candida utilis ferment respectively to the assimilability of ammonia nitrogen
2.6 times and 1.6 times of mother 1807.Show that saccharomycete SG60765 is good to the assimilative capacicy of ammonia nitrogen.
SG60765 plants of candida utili degradation gossypol ability characteristics are：At 37 DEG C, containing 60.84mgKg-1Gossypol
In cotton dregs, SG60765 to the degradation rate of gossypol up to 93.4%, respectively than saccharomyces cerevisiae CCTCC AY92003 and Candida utilis ferment
Female 1807 degradation rates high 54.8% and 23.7%, show that saccharomycete SG60765 is good to the degradation capability of gossypol.
SG60765 plants of PCR amplifications for carrying out 26S rDNA of candida utili are tested with sequencing, analysis shows that：Bacterial strain
The homology of the 26S rDNA of SG60765 and Candida utilis IFO 10707 has reached 99%, determines bacterial strain SG60765
For a kind of candida utili.
The invention also discloses a kind of SG60765 containing candida utili plants of probiotics composition, the probiotics
Composition main ingredient is SG60765 plants of candida utili and extracellular products.
It is characterized in that it contains following component, based on viable count ratio：
Candida utili 60%
Bacillus licheniformis 20%
Lactobacillus plantarum 20%
Wherein candida utili is CCTCC NO:M2015718, bacillus licheniformis are ACCC 19372, plant breast bar
Bacterium is CICC 20765.
Bacillus licheniformis is ACCC 19372 and lactobacillus plantarum be CICC 20765 is common common micro-organisms bacterium
Kind, it is published respectively in Chinese agriculture Microbiological Culture Collection administrative center and Chinese industrial Microbiological Culture Collection administrative center
In strain catalogue, in open state, scientific worker can ask for this two Culture Collection Center.
Probiotics composition disclosed by the invention is liquid or solid-state.Preferential, probiotics group disclosed by the invention
Conjunction object is solid-state.
The invention also discloses the method for preparing solid-state SG60765 probiotics and combinations thereof, this method includes as follows
Step：Include the following steps：
(1) actication of culture：SG60765 plants of candida utili is inoculated in sterilized liquid culture medium, 28~37 DEG C of shaking tables
Culture 18-24 hours, fluid nutrient medium composition is glucose 3%, and yeast extract 1%, peptone 1%, surplus is distilled water；
(2) solid culture：By the yeast liquid of activation culture by 10% mass than mounted box after combined inoculation solid medium,
30 DEG C or so of room temperature, 35 DEG C or so of product temperature is kept to cultivate 2 days and ferment, the preparation of solid medium is by wheat bran 96%, brown sugar
4%, with water material moistening to moisture content 40%, boiling is cold dissipate after；
(3) low temperature drying：Low temperature drying processing is carried out at 45 DEG C to the solid sample after fermentation, sample moisture content is less than
After 12%, carry out being packaged to be SG60765 probiotics；
(4) by weight by the SG6076 probiotics prepared, bacillus licheniformis 20% and plant breast bar is added
It is packed after microbial inoculum 20%, obtains the combination microbial inoculum of the probiotics containing SG60765, wherein bacillus licheniformis is ACCC 19372, is planted
Object lactobacillus is CICC 20765.
The application that the invention also discloses SG60765 plants of candida utili in fermentation cotton dregs.
The application that invention also discloses SG60765 plants of candida utili in liquid waste processing containing ammonia nitrogen.
Advantages of the present invention：
1, candida utili SG60765 plants provided by the invention are one plant of mutant bacterias being newly obtained by mutation breeding
Strain, the bacterial strain have very high ammonia nitrogen assimilative capacicy, and the assimilatory power than industrial common 1807 bacterial strain ammonia nitrogen is 1.6 times high；Than it
The assimilatory power of starting strain SG607 ammonia nitrogens is high by 12.1%.
2, the candida utili SG60765 plants of abilities with very strong degradation gossypol, than industrial common 1807 bacterium
Strain gossypol degradation rate is high by 36.1%；It is higher by 13.2% than its starting strain SG607 gossypol degradation rates.
3, have very high biomass, under liquid culture condi, compare candida utili for candida utili SG60765 plants
The biomass of ACCC20060 bacterial strains is 1.4 times high.
4, candida utili SG60765 plants for containing ammonia nitrogen cotton dregs carry out raw material fermentation when, the bacterial strain is to ammonia nitrogen
Assimilability and 90% or more is reached to the degradation rate of gossypol, shows excellent comprehensive performance, overcome currently on the market
The more single disadvantage of yeast strain function has apparent advantage in production.
5, when the combination microbial inoculum of SG60765 plants of Candida containing protein is used for fermentation cotton dregs, the aqueous soluble protein of fermentation cotton dregs contains
It is significantly improved when amount is than with single bacteria fermentation.I.e. candida utili SG60765 plants have stronger ammonia nitrogen assimilability, cotton
The ability of Phenol degradation rate and higher biomass, a combination thereof microbial inoculum not only stronger ammonia nitrogen assimilability and gossypol degradation capability, also
With the stronger ability for improving fermentation cotton dregs water-solubility protein.
Description of the drawings
SG60765 plants of 26S rDNA D1/D2 region sequence phylogenetic trees of Fig. 1 candida utili
Specific implementation mode
With reference to specific embodiment, the present invention is further explained.It should be appreciated that these embodiments are merely to illustrate
The present invention, and cannot limit the scope of the invention.
Embodiment
The selection and breeding and identification of the high ammonia nitrogen assimilability of 1 candida utili of embodiment, Cambodia Phenol degradation rate mutant strain SG60765
The selection and breeding of the high ammonia nitrogen assimilability mutant strain SG6076 of 1.1 candida utili
Using candida utili SG607 as starting strain, by the SG607 bacteria suspensions after ultraviolet irradiation after suitably diluting
It is applied on YPD solid mediums, when its bacterium colony is obvious, and the colony inoculation for therefrom choosing full, big circle is solid to YPD
On body culture medium, after it grows single bacterium colony, it is transferred in the 25mL culture bottles equipped with 7mLYPD fluid nutrient mediums, at 37 DEG C
Shaking table culture 5h under the conditions of 170rpm quantitatively moves into cultured each yeast liquid in 96 orifice plates, with microplate reader in wavelength
At 620nm, its OD value is measured.Therefrom choose 20 plants of larger bacterial strains of OD values, number is No. 1-No. 20 bacterial strains, is carried out further multiple
Sieve, 3 repetitions of each bacterial strain.5 plants of ammonia nitrogen assimilabilities are obtained altogether than the mutant strain that starting strain is significantly increased, wherein number is
The ammonia nitrogen assimilability of No. 6 mutant strains is up to 93.2%, and the ammonia nitrogen assimilability than starting strain improves 12.1%, this is dashed forward
Mutant is named as SG6076 (being shown in Table 1).
The measurement result of the ammonia nitrogen assimilative capacicy of 15 plant mutant bacterial strain of table
The selection and breeding of 1.2 candida utili Cambodia Phenol degradation rate mutant strain SG60765
In order to obtain the quality yeast bacterial strain for being provided simultaneously with high ammonia nitrogen assimilability and Cambodia's Phenol degradation rate, the present invention is according to micro-
Biological gene is mutated mutually independent principle, and carrying out second of ultraviolet light to the high ammonia nitrogen assimilability mutant strain SG6076 of acquisition lures
Become and use high-throughput screening method, therefrom filters out the mutant strain that there is high degradation rate to gossypol.Specific method is：With
SG6076 is starting strain, and the SG6076 bacterium solutions after ultraviolet irradiation are passed through dilution appropriate, it is solid to be then coated on gossypol acetate
On body culture medium, after being protected from light culture for 24 hours, chooses full, big and circle bacterium colony, after activated, be inoculated in and fill 10mL and contain
1000mg·L-1In the culture medium of gossypol acetate, shaking table culture 5h under the conditions of 37 DEG C, 170rpm takes the bacterium solution of 200 μ L to nothing
In 96 orifice plates of bacterium, at wavelength 620nm, OD is measured with microplate reader620Value.Pass through OD620It is worth size and carries out primary dcreening operation, obtains 4 altogether
The mutant strain (table 2) that strain is significantly increased than starting strain OD values.After this 4 plant mutant strain and starting strain activation, with 10%
Inoculum concentration is inoculated with, and fermentation condition is moisture 42%, 37 DEG C of temperature, time 36h, carries out solid cotton dregs fermentation, passes through measurement
It carries out secondary screening to the degradation rate of gossypol.As can be seen from Table 3, the gossypol degradation rate of No. 65 mutant strains is up to 93.6%,
11.5% is improved than starting strain.It is SG60765 that No. 65 mutant strains, which are named as number,.
Gossypol degradation capability is compared in 24 plant mutant strain of table
The present inventor will pass through the one of ultraviolet mutagenesis selection and breeding twice plant of new SG60765 plants of (Candida of candida utili
Utilis SG60765) it is preserved in China typical culture collection center, deposit number CCTCCNO:2015718.Preservation day
Phase is on December 3rd, 2015.China typical culture collection center is located at Wuhan City, Hubei Province Wuchang District Wuhan University in the school, electricity
Words：027-68752319, EMAIL：cctcc@whu.edu.cn.
The identification of 1.3 SG60765 plants of candida utili
Pass through morphological feature observation, physiological and biochemical test and 26S rDNA D1/D2 regional gene the sequencing results pair
SG60765 is identified.In authentication, this experimental selection Angel thermotolerant ethanol fermentation yeast TRADY, candida utili CICC
1807 bacterial strains as a contrast.Saccharomyces cerevisiae CCTCC AY92003 are common General Microbiological Culture, are published in Chinese Typical Representative culture
Object collection catalogue；Candida utili CICC 1807 comes from Chinese industrial Microbiological Culture Collection administrative center, has gone up mesh
Record, open state, scientific worker can ask for.
1.3.1 candida utili SG60765 plant shapes state observation of characteristics
In YPD cultured on solid medium 1d-2d, bacterium colony is full circle, and center protrudes, milky, cheese shape, table
Face is smooth, moistening, adhesion, easy picking.In YPD fluid nutrient medium culture 12h-14h, cell is oval, slightly round, part cell
End to end is in together typical false filiform, and size is (2.5-3.5) μ m (6.5-8.5) μm.
1.3.2 it is analyzed by 26S rDNA D1/D2 regional sequences and carries out Phylogenetic identification
Pcr amplification reaction, the areas amplification yeast strain 26S rDNA D1/D2 are done by template of the genomic DNA of yeast strain
Domain, amplimer use universal primer NS1 (5'-GTAGTCATATGCTTGTCTC-3') and NS4 (5'-
CTTCCGTCAATTCCTTTAAG-3')。
PCR reaction systems (25 μ L)：10 × PCR buffer solutions (Mg containing 20mmol/L2+) 2.5 μ L, primer NS1/NS4 (10 μ
Mol/L) each 1 μ L, template DNA (about 50ng/mL) 1.8/2.8 μ L, dNTP (10mmol/L each) 0.5 μ L, Taq enzyme (5U/ μ L)
0.2 μ L, 18/17 μ L of distilled water.
PCR amplification condition：94 DEG C of pre-degeneration 5min, 94 DEG C of denaturation 40s, 50 DEG C of annealing 40s, 72 DEG C of extension 60s, 30 are followed
After ring, 72 DEG C of extension 5min.Gained PCR product serves the raw work bioengineering in sea and purifies and be sequenced, sequencing primer with above-mentioned NL1,
NL4。
After being sequenced by molecule, it is found that the 26S rDNA's of bacterial strain SG60765 and Candida utilis IFO10707 is same
Source property has reached 99%, meets standard of the difference no more than 1% between the fixed interior different strains of the same race of Kuttzman＆Robnett,
Determine that bacterial strain SG60765 is a kind of candida utili.Fig. 1 is the system hair done according to 26S rDNA D1/D2 regional sequences
Educate tree.
1.3.3 candida utili SG60765 plants of bio-chemical characteristics measurement result
SG60765 bacterial strains the results are shown in Table 3 to the utilization of sugar.SG60765 bacterial strains can utilize 6 kinds of sugar such as sucrose.
The fermentation test result of 3 candida utili SG60765 different carbon sources of table
2. candida utili SG60765 of embodiment is to the assimilative capacicy of ammonia nitrogen and the effect of the high ammonia-nitrogen content waste liquid of processing
Analysis
The comparison result of 2.1 different yeast strain ammonia nitrogen assimilabilities
By the 7 saccharomycete seed liquor such as SG60765 with 5% inoculum concentration be transferred to respectively 3 it is independent be equipped with 100mL ferment
In the 250mL triangular flasks of female bacteria fermentation culture medium, shaker fermentation for 24 hours, measures fermented liquid supernatant ammonia under the conditions of 37 DEG C of 170rpm
Nitrogen content, more different yeast strains are to the assimilability of ammonia nitrogen, and the results are shown in Table 4, and mutant strain SG6076 is than its starting strain
The ammonia nitrogen assimilability of SG607 improves 13.1%, to ammonia nitrogen assimilability without apparent poor between mutant strain SG6076 and SG60765
It is different.
Candida tropicalis CICC 1254 is be purchased from Chinese industrial Microbiological Culture Collection administrative center strain library one plant
Bacterial strain for fermentation production of fodder yeast.Candida utili ACCC20060 is to be purchased from Chinese agriculture Microbiological Culture Collection
One plant of administrative center's strain is used for the bacterial strain of fermentation production of fodder yeast.Saccharomyces cerevisiae CCTCC AY92003 are common common
Microorganism fungus kind is published in China typical culture collection center catalogue, and in open state, scientific worker can be to Chinese allusion quotation
Type culture collection is asked for.
The comparison of the different yeast strain ammonia nitrogen assimilabilities of table 4
2.2 different yeast strains handle the effect analysis of high ammonia-nitrogen content waste liquid
Candida utili SG60765, SG607 and the ACCC20060 for choosing the above 3 plant height ammonia nitrogen assimilabilities carry out high ammonia
The fermentation scale-up of nitrogen content waste liquid is tested, and this time fermentation is using the culture solution for filling 1L high ammonia-nitrogen contents in 3L triangular flasks.By 10%
Volume ratio inoculum concentration is inoculated with each yeast liquid in 240.0mgL containing ammonia nitrogen respectively-1Waste liquid in, shaken under the conditions of 37 DEG C of 170rpm
It after bed culture for 24 hours, is centrifuged in 4000rpm, 5min, collects thalline, 105 DEG C of drying weigh, and measure thalline crude protein content.
As can be seen from Table 5, SG60765 has further raising in the case where expanding condition of culture to ammonia nitrogen assimilability, reaches 96.6%,
It is higher by 10.2% than the ammonia nitrogen assimilability of SG607 and ACCC20060 bacterial strains respectively and 15.9%.With SG607 and ACCC20060 phases
Than the biomass and crude protein content of SG60765 is higher, respectively reaches 3.83gL-1With 51.0%.Identical fermentation condition
Under, the biomass of SG60765 yeast thalline is 1.4 times of ACCC20060 bacterial strain biomass.
The effect that the different yeast strains of table 5 handle high ammonia-nitrogen content waste liquid compares
The capability analysis of gossypol and assimilation ammonia nitrogen in embodiment 3.SG60765 strain fermentations degradation cotton dregs
By saccharomyces cerevisiae CCTCC AY92003, candida tropicalis CICC 1254；Candida utili CICC1807,
ACCC20060, SG607, SG6076, SG60765 are inoculated with addition 1% by inoculum concentration 10% after totally 7 plants of yeast activate respectively respectively
The cotton dregs of ammonium sulfate carry out the experiment of solid-state raw material fermentation and compare, and fermentation condition is：Fermentation material starting moisture 42%, fermentation temperature 37
DEG C, fermentation time 36h.The ammonia nitrogen and gossypol measured respectively with Kjeldahl's method and liquid chromatography in the front and back cotton dregs of fermentation contains
Amount, and calculate the ammonia nitrogen assimilability and gossypol degradation rate of each bacterial strain.The gossypol content of measuring cotton dregs raw material is 60.84mg
Kg-1.As can be seen from Table 6, candida utili SG60765 is up to 93.4% to the degradation rate of gossypol, respectively than ferment of making wine
Female CCTCC AY92003 and 1807 degradation rate of candida utili high 54.8% and 23.7%, than its starting strain SG6076 high
10.0%, and SG60765 mutant strains are similar to SG6076 to the assimilability of ammonia nitrogen, show that the mutant strain is obtaining high gossypol drop
The character of its high ammonia nitrogen assimilative capacicy is still remained while solution ability.Between same candida utili different strains such as
There is also significant differences for degradation capabilities of the CICC1807 and ACCC20060 to gossypol.
Comparison of the different yeast strains of table 6 to ammonia nitrogen assimilability and gossypol degradation rate
4 SG60765 bacterial strains of embodiment are imitated with bacillus licheniformis and lactobacillus plantarum by different proportion mixed fermentation cotton dregs
Fruit is compared
It is CICC 20765 by bacillus licheniformis ACCC 19372, candida utili SG60765 and lactobacillus plantarum
After activation, respectively with viable count ratio for 3：1：0、2：1:0、1：1:0、1：2:0、1：3:0 and 1：3:1 carries out combined inoculation hair
Ferment, cotton dregs fermentation condition are moisture 42%, 37 DEG C of temperature, time 36h.As can be seen from Table 7, with saccharomycete in mixed bacteria
Inoculum concentration constantly increase, gossypol degradation rate constantly increases, when bacillus licheniformis ACCC 19372 and candida utili
SG60765 inoculative proportions are 1:When 3, gossypol degradation rate reaches highest, is 91.3%.Show candida utili SG6076 to cotton
The degradation of phenol is more than bacillus licheniformis.Lactobacillus plantarum, that is, ACCC19372 is added in microbial inoculum when combining：SG60765:
CICC20765 is 1:3:When 1, the gossypol degradation rate of fermentation cotton dregs is up to 91.5%, and the pH value of fermentation material is down to 5.0 by 6.0, table
The bright combination microbial inoculum containing lactobacillus plantarum is not apparent from the degradation rate for reducing gossypol, but can dramatically increase the acidity of fermentation cotton dregs,
This is conducive to the palatability and storage time that increase feed.
7 SG60765 of table and influence of the bacillus licheniformis different mixing proportion to gossypol degradation rate
The preparation method of 5 SG60765 probiotics of embodiment and combinations thereof
5.1 actication of culture：SG60765 plants of candida utili is inoculated in sterilized liquid culture medium (glucose 3%, yeast
Cream 1%, peptone 1%) in, 28~37 DEG C of shaking table cultures 18-24 hours；
5.2 solid culture：The dispensing of solid culture is：Wheat bran 96%, brown sugar 4% are steamed with water material moistening to moisture content 40%
After boiling cold dissipate, by 5.1 activation culture, 2 primary yeast bacterium solution by mounted box after 10% inoculum concentration volume ratio combined inoculation, room temperature 30 is kept
DEG C or so.35 DEG C or so of product temperature is cultivated fermentation in 2 days and is finished；
5.3 low temperature drying：Low temperature drying processing is carried out at 45 DEG C to the solid sample after fermentation, sample moisture content is less than
After 12%, carry out being packaged to be SG60765 probiotics；
The SG60765 probiotics prepared are added bacillus licheniformis and plant breast bar by 5.4 by a certain percentage respectively
It is packed to get to the combination microbial inoculum of the probiotics containing SG60765 after microbial inoculum；
5.5 saccharomycete preparation compositions measurement results：The probiotics combination prepared using dilution plate determination experiment room
Candida utili bacterium viable count reaches 1,500,000,000/g or so in object.Candida utili SG60765：ACCC 19372：CICC
20765 viable count ratios are 6：2：2.
Application of the 6 SG60765 single plant fermenting agents of embodiment and combinations thereof in fermentation cotton dregs
6.1 SG60765 single plant fermenting agents and combinations thereof are used for cotton dregs fermentation test result
It is 40% that SG60765 single fermentation microbial inoculums and combinations thereof are inoculated in moisture with 2% inoculum concentration respectively
In the solid cotton dregs of 100Kg, after raw material fermentation 40h, crushed after 60 DEG C of drying of baking oven.By data in table 8 it is found that using single
Crude protein content has increased separately 7.25% and 7.61% after microbial inoculum and combination microbial inoculum fermentation cotton dregs, and gossypol degradation rate is respectively
90.16% and 90.21%, water-solubility protein is respectively 9.89% and 12.45%.Using the cotton dregs of combination bacteria fermentation than single
The water-soluble protein content of the cotton dregs of bacteria fermentation improves 25.88%, shows to combine microbial inoculum in the water-soluble egg for increasing cotton dregs
White aspect has a clear superiority.Cotton dregs after fermentation all have dense yeast fragrance, can increase the palatability of feed.
The effect of 8 single microbial inoculum of table and combination microbial inoculum fermentation cotton dregs compares
6.2 fermentation cotton dregs raise table hens test result and analysis
In order to test the feeding effect of the cotton dregs with combination bacteria fermentation, 100 are selected to go out precious 500 Broiler chicks of shell health section
It is public young, 5 groups are randomly divided into, every group of 4 repetitions are each to repeat 5.Five groups feed respectively dregs of beans, 50% cotton dregs Substitution for Soybean Meal,
100% cotton dregs Substitution for Soybean Meal, 50% fermentation cotton dregs Substitution for Soybean Meal, 100% fermentation cotton dregs Substitution for Soybean Meal.Hen house temperature is controlled on the 1st week
System reduces weekly 3 DEG C, until off-test later at 35 DEG C.Each group test chicken is freely eaten and drinking-water.The daily morning 7:00 charging
And water, experimental period are 21 days.Without chicken death during experiment.Test result is shown in Table 9.From table 9, cotton dregs are right whether fermentation
Average daily gain and feedstuff-meat ratio have pole to significantly affect (p≤0.001), and replacement amount (50% and 100%) pole of dregs of beans significantly affects
Feed intake.It is not made significant difference with the production performance of 50% couple, 0~21 Day-old Broiler Chickens of fermentation cotton dregs Substitution for Soybean Meal.Show to use group
The cotton dregs for closing bacteria fermentation have the function of preferable Substitution for Soybean Meal.
9 liang of factors of table (cotton dregs and fermentation cotton dregs) two horizontal (Substitution for Soybean Meal 50% and 100%) test 0~21 age in days of broiler chicken
Production performance compares
Claims (8)
1. one plant of candida utili (Candida utilis) SG60765, which is characterized in that the bacterial strain is preserved in Chinese allusion quotation
Type culture collection, deposit number are CCTCC NO:M2015718, by the candida utili SG607 detached through soil
Mutagenesis generates.
2. a kind of microorganism probiotic agent containing SG60765 plants of candida utili described in claim 1, it is characterised in that
Its active constituent is SG60765 plants of thalline of candida utili and its metabolite.
3. a kind of probiotics composition containing bacterial strain described in claim 1.
4. according to the probiotics composition described in claim 3, it is characterised in that it contains following component, by viable count ratio
Meter：
Candida utili 60%
Bacillus licheniformis 20%
Lactobacillus plantarum 20%
Wherein candida utili is CCTCC NO:M2015718, bacillus licheniformis are ACCC 19372, and lactobacillus plantarum is
CICC 20765。
5. according to the probiotics composition described in claim 3, it is characterised in that the probiotics composition is solid
State microbial inoculum.
6. the method for preparing the probiotics composition described in claim 4, includes the following steps：
(1) actication of culture：SG60765 plants of candida utili is inoculated in sterilized liquid culture medium, 28~37 DEG C of shaking table cultures
18-24 hours, fluid nutrient medium composition was glucose 3%, and yeast extract 1%, peptone 1%, surplus is distilled water；
(2) solid culture：The yeast liquid of activation culture is kept by 10% mass than mounted box after combined inoculation solid medium
30 DEG C or so of room temperature, 35 DEG C or so of product temperature are cultivated 2 days and are fermented, and the preparation of solid medium is to use wheat bran 96%, brown sugar 4%
Water material moistening to moisture content 40%, boiling is cold dissipate after be prepared；
(3) low temperature drying：Low temperature drying processing is carried out at 45 DEG C to the solid sample after fermentation, sample moisture content is less than 12%
Afterwards, it carries out being packaged to be SG60765 probiotics；
(4) by weight by the SG6076 probiotics prepared, bacillus licheniformis 20% and lactobacillus plantarum agent is added
It is packed after 20%, obtains the combination microbial inoculum of the probiotics containing SG60765, wherein bacillus licheniformis is ACCC 19372, plant breast
Bacillus is CICC 20765.
7. applications of the candida utili SG60765 in fermentation cotton dregs described in claim 1.
8. applications of the candida utili SG60765 in liquid waste processing containing ammonia nitrogen described in claim 1.
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| CN108048338B (en) * | 2017-12-31 | 2021-08-13 | 湖北至正天辰生物科技有限公司 | Saccharomyces cerevisiae and application thereof in aquaculture |
| CN109607823A (en) * | 2018-12-26 | 2019-04-12 | 齐齐哈尔龙江阜丰生物科技有限公司 | The method for administering threonine mother liquor and separation and Extraction albumen |
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| CN110591943B (en) * | 2019-09-05 | 2021-12-17 | 武汉轻工大学 | Bacillus subtilis capable of producing complex enzyme, composition and application thereof |
| CN111903838A (en) * | 2020-08-13 | 2020-11-10 | 厦门惠盈动物科技有限公司 | Yeast culture and compound lactobacillus preparation and preparation method thereof |
| CN114836331B (en) * | 2022-04-26 | 2023-04-25 | 内蒙古工业大学 | Candida utilis and application thereof |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178035A (en) * | 2011-04-22 | 2011-09-14 | 河南宏翔生物科技有限公司 | Method for fermenting and decomposing gossypol in cottonseed meal by composite strains |
| CN102823726A (en) * | 2012-09-07 | 2012-12-19 | 北京大北农科技集团股份有限公司 | Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity |
-
2015
- 2015-12-17 CN CN201510952295.7A patent/CN105385612B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178035A (en) * | 2011-04-22 | 2011-09-14 | 河南宏翔生物科技有限公司 | Method for fermenting and decomposing gossypol in cottonseed meal by composite strains |
| CN102178035B (en) * | 2011-04-22 | 2012-09-05 | 河南宏翔生物科技有限公司 | Method for fermenting and decomposing gossypol in cottonseed meal by composite strains |
| CN102823726A (en) * | 2012-09-07 | 2012-12-19 | 北京大北农科技集团股份有限公司 | Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity |
Non-Patent Citations (4)
| Title |
|---|
| Xiao-Yan Weng.Biodegradation of free gossypol by a new strain of Candida tropicalis under solid state fermentation: Effects of fermentation parameters.《Process Biochemistry》.2006,第41卷(第7期),第1663-1668页. * |
| 一株降解棉酚的酵母菌培养特征及发酵效果分析;周慧林 等;《武汉轻工大学学报》;20140331;第33卷(第1期);第14-16页 * |
| 耐高温酵母菌高氨氮同化率菌株的筛选和发酵条件研究;曹玉飞 等;《武汉轻工大学学报》;20150630;第34卷(第2期);第1-5页 * |
| 耐高温酵母菌高氨氮同化率菌株的筛选和发酵条件研究;曹玉飞;《中国优秀硕士学位论文全文数据库 基础科学辑》;20150415(第04期);第1-44页 * |
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