CN110250320A - The preparation method of radix pseudostellariae ferment - Google Patents
The preparation method of radix pseudostellariae ferment Download PDFInfo
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- CN110250320A CN110250320A CN201910598197.6A CN201910598197A CN110250320A CN 110250320 A CN110250320 A CN 110250320A CN 201910598197 A CN201910598197 A CN 201910598197A CN 110250320 A CN110250320 A CN 110250320A
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- radix pseudostellariae
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- tealeaves
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- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 30
- 241000894006 Bacteria Species 0.000 claims abstract description 26
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 238000009835 boiling Methods 0.000 claims abstract description 17
- 239000001963 growth medium Substances 0.000 claims abstract description 14
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 14
- 150000002482 oligosaccharides Chemical class 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 10
- 239000004310 lactic acid Substances 0.000 claims abstract description 10
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 238000003756 stirring Methods 0.000 claims abstract description 7
- 235000013379 molasses Nutrition 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims abstract description 6
- 239000008399 tap water Substances 0.000 claims abstract description 6
- 235000020679 tap water Nutrition 0.000 claims abstract description 6
- 238000000855 fermentation Methods 0.000 claims description 11
- 230000004151 fermentation Effects 0.000 claims description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 8
- 244000235858 Acetobacter xylinum Species 0.000 claims description 7
- 235000002837 Acetobacter xylinum Nutrition 0.000 claims description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 6
- 244000269722 Thea sinensis Species 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 5
- 239000000052 vinegar Substances 0.000 claims description 5
- 235000021419 vinegar Nutrition 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims description 4
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims description 4
- 235000020334 white tea Nutrition 0.000 claims description 4
- 241000193749 Bacillus coagulans Species 0.000 claims description 3
- 241000222178 Candida tropicalis Species 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 244000199866 Lactobacillus casei Species 0.000 claims description 3
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 3
- 229940054340 bacillus coagulans Drugs 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 claims description 3
- 229940107187 fructooligosaccharide Drugs 0.000 claims description 3
- 235000021255 galacto-oligosaccharides Nutrition 0.000 claims description 3
- 150000003271 galactooligosaccharides Chemical class 0.000 claims description 3
- 229940017800 lactobacillus casei Drugs 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000002023 wood Substances 0.000 claims description 3
- 244000283763 Acetobacter aceti Species 0.000 claims description 2
- 235000007847 Acetobacter aceti Nutrition 0.000 claims description 2
- 241000235645 Pichia kudriavzevii Species 0.000 claims description 2
- 235000009569 green tea Nutrition 0.000 claims description 2
- 235000013616 tea Nutrition 0.000 claims description 2
- 235000009508 confectionery Nutrition 0.000 claims 1
- 239000006071 cream Substances 0.000 claims 1
- -1 synanthrin Chemical class 0.000 claims 1
- 241001596950 Larimichthys crocea Species 0.000 description 12
- 241000251468 Actinopterygii Species 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 150000003384 small molecules Chemical class 0.000 description 4
- 244000182067 Fraxinus ornus Species 0.000 description 3
- 235000002917 Fraxinus ornus Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000248484 Ichthyophthirius Species 0.000 description 2
- 240000001046 Lactobacillus acidophilus Species 0.000 description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 2
- 240000004980 Rheum officinale Species 0.000 description 2
- 235000008081 Rheum officinale Nutrition 0.000 description 2
- 235000006468 Thea sinensis Nutrition 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 235000020279 black tea Nutrition 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 230000001408 fungistatic effect Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- HHXYJYBYNZMZKX-UHFFFAOYSA-N 3,4:15,16-diepoxy-7-oxo-13(16),14-clerodadien-20,12-olide-(3alpha,4alpha)-form Natural products C12CCC3C4(C)CCCC(C)(C)C4CCC3(C)C1(C)CCC1C2(C)CCC1C(=C)C HHXYJYBYNZMZKX-UHFFFAOYSA-N 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 240000009056 Saccharum arundinaceum Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Birds (AREA)
- Sustainable Development (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention provides a kind of preparation methods of radix pseudostellariae ferment, comprising the following steps: 5-20 parts of radix pseudostellariae powders is broken to 20-40 mesh, the water of 5 times of addition obtains the pretreatment object of radix pseudostellariae for 120 minutes in 60 degrees Celsius of boilings；5-20 portions of tealeaves are crushed to 20-40 mesh, the water of 5 times of addition obtains the pretreatment object of tealeaves for 30 minutes in 90 degrees Celsius of boilings；Be added the pretreatment object of radix pseudostellariae, tealeaves in the fermenter, the cane molasses of 80~120 parts of weight, the oligosaccharide of 5~15 parts of weight, 700~1000 parts of weight tap water, kept the temperature under 90~110 degree after mixing, then be down to 30~40 degrees Celsius and obtain culture medium；Be added in culture medium obtained into fermentor the saccharomycete of 45~55 parts of weight, the acetic acid bacteria of 45~55 parts of weight, 45~55 parts of weight lactic acid bacteria, it is cultivated 40~70 days at 25-35 degrees Celsius, compressed air is passed through to be cultivated, compressed air intake is 0.1~0.3vvm, and is spaced predetermined time stirring.
Description
Technical field
The invention belongs to field of feed additive technology, in particular to a kind of preparation method of radix pseudostellariae ferment.
Background technique
With the rapid development of Larimichthys crocea aquaculture, the continuous expansion of scale and cultured area, cage culture rheum officinale are cultivated
The disease of fish also constantly occurs, and frequency and scale are all in rise year by year trend.In 2009, there is " township of Larimichthys crocea " good reputation
Extensive Larimichthys crocea ichthyophthirius occurs for Bay, Ningde, Fujian Province city, and the disease incidence in Jiaocheng District has reached 100% unexpectedly, and the whole city loses more than 2
Hundred million yuan, the sudden and violent of large-scale Larimichthys crocea ichthyophthirius also once occurred in the white sand angle sea area of the town Ningde City Xiapu County Xi Nan in 2005
Hair, causes about 4000 tons of Larimichthys crocea death, accounts about the 8% of Fujian the whole province Larimichthys crocea annual output, therefore raiser loses 6000
More ten thousand yuan.Therefore, how to increase the immunity of Larimichthys crocea, reduce disease incidence, reducing the death rate becomes important research side
To.
Summary of the invention
The present invention provides a kind of preparation methods of radix pseudostellariae ferment, can effectively solve the above problems.
The present invention is implemented as follows:
A kind of preparation method of radix pseudostellariae ferment, wherein the following steps are included:
The radix pseudostellariae powder of 5-20 parts of weight is broken to 20-40 mesh, the water of 4~10 times of weight is added by the pretreatment of radix pseudostellariae
The pretreatment object of radix pseudostellariae is obtained in 50~70 degrees Celsius of lower boilings；
The tealeaves of 5-20 parts of weight is crushed to 20-40 mesh by the pretreatment of tealeaves, and the water of 4~10 times of weight is added 80
~95 degrees Celsius of lower boilings obtain the pretreatment object of tealeaves；
The pretreatment object of radix pseudostellariae, tealeaves, the sugarcane of 80~120 parts of weight is added in the preparation of culture medium in the fermenter
Molasses, the oligosaccharide of 5~15 parts of weight, 700~1000 parts of weight tap water, kept the temperature under 90~110 degree after mixing, then drop
Culture medium is obtained to 30~40 degrees Celsius；
Saccharomycete, 45~55 parts of weights of 45~55 parts of weight are added into fermentor for inoculation fermentation in culture medium obtained
The lactic acid bacteria of the acetic acid bacteria of amount, 45~55 parts of weight is cultivated 40~70 days at 25-35 degrees Celsius, is passed through compressed air and is trained
It supports, compressed air intake is 0.1~0.3vvm, and is spaced predetermined time stirring, stops fermentation after the time, while stirring
Tank is put, sterile sealing is filling, packed products.
As further improved, the tealeaves is one or more of white tea, green tea, black tea etc..
As further improved, the radix pseudostellariae is 10 parts, and the tealeaves is 10 parts.
As further improved, the oligosaccharide be wood oligose, manna oligosacchride, soyabean oligosaccharides, fructooligosaccharide, synanthrin,
One or more of galactooligosaccharide etc..
As further improved, the saccharomycete is saccharomyces cerevisiae, Bu Ladi yeast, candida tropicalis, Cruise
The Mixed Microbes of at least two equal proportions in Candida etc..
As further improved, the acetic acid bacteria is acetobacter xylinum, intends acetobacter xylinum, and glucose vinegar bacillus acetifies vinegar
The Mixed Microbes of at least two equal proportions in bacillus etc..
As further improved, the lactic acid bacteria is lactobacillus acidophilus, Lactobacillus casei, and lactobacillus bulgaricus coagulates
Tie the Mixed Microbes of at least two equal proportions in bacillus etc..
The beneficial effects of the present invention are: not needing to be separated after pretreatment respectively and being directly added into ferment radix pseudostellariae, tealeaves
Plain strain carries out inoculation fermentation processing, reduces processing cost；Subsequent fermentation process can be by effective component in radix pseudostellariae, tealeaves
Macromolecular substances are converted into small-molecule substance, rapid-action since small-molecule substance has higher pharmacological activity, and are easier
It is absorbed by animal body, is conducive to animal body and radix pseudostellariae ferment is effectively absorbed and utilized, significantly increase exempting from for Larimichthys crocea
Epidemic disease power reduces disease incidence, improves survival rate.
Detailed description of the invention
It, below will be to use required in embodiment in order to illustrate more clearly of the technical solution of embodiment of the present invention
Attached drawing be briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not to be seen as
It is the restriction to range, it for those of ordinary skill in the art, without creative efforts, can be with root
Other relevant attached drawings are obtained according to these attached drawings.
Fig. 1 is the flow chart of the preparation method of radix pseudostellariae ferment provided in an embodiment of the present invention.
Fig. 2-5 is the Escherichia coli fungistatic effect figure after the ferment provided in an embodiment of the present invention using radix pseudostellariae.
Specific embodiment
To keep the purposes, technical schemes and advantages of embodiment of the present invention clearer, implement below in conjunction with the present invention
The technical solution in embodiment of the present invention is clearly and completely described in attached drawing in mode, it is clear that described reality
The mode of applying is some embodiments of the invention, rather than whole embodiments.Based on the embodiment in the present invention, ability
Domain those of ordinary skill every other embodiment obtained without creative efforts, belongs to the present invention
The range of protection.Therefore, the detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit below and is wanted
The scope of the present invention of protection is sought, but is merely representative of selected embodiment of the invention.Based on the embodiment in the present invention,
Every other embodiment obtained by those of ordinary skill in the art without making creative efforts belongs to this
Invent the range of protection.
In the description of the present invention, term " first ", " second " are used for description purposes only, and should not be understood as instruction or dark
Show relative importance or implicitly indicates the quantity of indicated technical characteristic.The feature of " first ", " second " is defined as a result,
It can explicitly or implicitly include one or more of the features.In the description of the present invention, the meaning of " plurality " is two
It is a or more than two, unless otherwise specifically defined.
Shown in referring to Fig.1, the embodiment of the present invention provides a kind of preparation method of radix pseudostellariae ferment, comprising the following steps:
The radix pseudostellariae powder of 5-20 parts of weight is broken to 20-40 mesh, 4~10 times of weight is added by S1, the pretreatment of radix pseudostellariae
Water obtains the pretreatment object of radix pseudostellariae in 50~70 degrees Celsius of lower boilings；
The tealeaves of 5-20 parts of weight is crushed to 20-40 mesh by S2, the pretreatment of tealeaves, and the water that 4~10 times of weight are added exists
80~95 degrees Celsius of lower boilings obtain the pretreatment object of tealeaves；
S3, the preparation of culture medium are added the pretreatment object of radix pseudostellariae, tealeaves in the fermenter, 80~120 parts of weight it is sweet
Cane molasses, the oligosaccharide of 5~15 parts of weight, 700~1000 parts of weight tap water, kept the temperature under 90~110 degree after mixing, then
It is down to 30~40 degrees Celsius and obtains culture medium；
S4, inoculation fermentation, be added in culture medium obtained into fermentor 45~55 parts of weight saccharomycete, 45~55 parts
The lactic acid bacteria of the acetic acid bacteria of weight, 45~55 parts of weight is cultivated 40~70 days at 25-35 degrees Celsius, is passed through compressed air progress
Culture, compressed air intake are 0.1~0.3vvm, and are spaced predetermined time stirring, stop fermentation, side stirring after the time
While putting tank, sterile sealing is filling, packed products.
In step sl, it is preferred that the radix pseudostellariae powder of 8-12 parts of weight is broken to 20-40 mesh.In the present embodiment, by 10 parts
The radix pseudostellariae powder of weight is broken to 20-40 mesh.When boiling temperature is excessively high, although quickly its internal component boiling can be come out,
But it is easily destroyed its effective component.Therefore, it is preferred that the water of 4~6 times of weight is added in 55~65 degrees Celsius of lower boilings 1~5
Hour obtains the pretreatment object of radix pseudostellariae.In the present embodiment, the water of 5 times of weight is added, and 2 hours of boiling are obtained at 60 c
To the pretreatment object of radix pseudostellariae.It is appreciated that by the selection of temperature, can under the precondition for not destroying effective component,
Quickly the effective component boiling of radix pseudostellariae is come out.
In step s 2, it is preferred that the tealeaves of 8-12 parts of weight is crushed to 20-40 mesh.The tealeaves can be white tea, green
One or more of tea, black tea etc..Preferably, the tealeaves is white tea.When boiling temperature is excessively high, although can quickly by
Its internal component boiling comes out, but is easily destroyed its effective component.Therefore, it is preferred that be added the water of 4~6 times of weight 80~
95 degrees Celsius of lower boilings obtain the pretreatment object of tealeaves for 10~60 minutes.In the present embodiment, the water that 5 times of weight are added is Celsius 90
Spend the pretreatment object that lower boiling obtains tealeaves for 30 minutes.
In step s3, the oligosaccharide is wood oligose, manna oligosacchride, soyabean oligosaccharides, fructooligosaccharide, synanthrin, oligomeric half
One or more of lactose etc..Preferably, the oligosaccharide is manna oligosacchride, the combination of galactooligosaccharide equal proportion.It is described low
Glycan is commercially available usual sources.
Additionally, it is preferred that, the cane molasses of 95~105 parts of weight, the oligosaccharide of 8~12 parts of weight, 780~820 are added
The tap water of part weight, keeps the temperature 10~60 minutes under 95~105 degree after mixing, then be down to 30~40 degrees Celsius and cultivated
Base.In the present embodiment, be added the cane molasses of 100 parts of weight, the oligosaccharide of 10 parts of weight, 800 parts of weight tap water, mix
20 minutes are kept the temperature under 100 degree afterwards, then is down to 35 degrees Celsius and obtains culture medium.Above-mentioned sterilization treatment, can will be in mixture
Bacterium all kills.
In step s 4, it is preferred that be added in culture medium obtained into fermentor 48~52 parts of weight saccharomycete, 48
The lactic acid bacteria of the acetic acid bacteria of~52 parts of weight, 48~52 parts of weight is cultivated 55~65 days at 28-32 degrees Celsius.In the present embodiment,
Be added in culture medium obtained into fermentor the saccharomycete of 50 parts of weight, the acetic acid bacteria of 50 parts of weight, 50 parts of weight lactic acid
Bacterium is cultivated 60 days at 30 degrees Celsius.
Compressed air is passed through to be cultivated, compressed air intake is 0.1~0.3vvm,
The saccharomycete is saccharomyces cerevisiae, Bu Ladi yeast, candida tropicalis, in candida krusei etc. at least
The Mixed Microbes of two kinds of equal proportions.Preferably, the saccharomycete is saccharomyces cerevisiae, Bu Ladi yeast equal proportion Mixed Microbes.Above-mentioned bacterium
Kind is commercially available usual sources.
The acetic acid bacteria is acetobacter xylinum, quasi- acetobacter xylinum, glucose vinegar bacillus, at least two in acetobacter aceti etc.
The Mixed Microbes of equal proportion.Preferably, the acetic acid bacteria is acetobacter xylinum, glucose vinegar bacillus equal proportion Mixed Microbes.Above-mentioned strain
It is commercially available usual sources.
The lactic acid bacteria is lactobacillus acidophilus, Lactobacillus casei, lactobacillus bulgaricus, in bacillus coagulans etc. extremely
The Mixed Microbes of few two kinds of equal proportions.Preferably, the lactic acid bacteria is lactobacillus bulgaricus, the mixing of bacillus coagulans equal proportion
Bacterium.Above-mentioned strain is commercially available usual sources.
Fig. 2-5 is the effect picture that the radix pseudostellariae ferment prepared using the embodiment of the present invention is generated.Wherein, Fig. 2-5 and table 1 are
Escherichia coli fungistatic effect figure (O157, O139, K88, K99).From table 1 it follows that radix pseudostellariae ferment to various bacteriums all
There is apparent inhibitory effect, diameter is inhibited to be all larger than >=15mm.
Table 1 is radix pseudostellariae ferment to various Bacteria suppression tables
The radix pseudostellariae ferment that the embodiment of the present invention is prepared Larimichthys crocea cultivation application in, by radix pseudostellariae ferment in
Feed mixing can feed practical.Specifically, as follows:
Test is located at inland sea farm at the one of Fujian Xiapu, which is that traditional Larimichthys crocea concentrates culturing area, wind
Unrestrained tranquil, aquaculture model is arranged in fishing.
Test grouping:
Test is divided to big specification and two kinds of cultivation objects of small dimension, wherein big specification is the left and right again average 50g, it is last year fingerling
Continue this year to cultivate.Small dimension is current year breeding fingerling, and the average a length of 4.4cm of body, weight is in 1.5g or so.Control group and test
Group is treated with a certain discrimination as following.
(1) control group: conventional feeding；
(2) 5% this product test group: is added in conventional bait.When using chilled feeding, this product is added in chilled together
It is homogenized, concentrates feeding again after placing 30 minutes；When using mixed feed, this product is added in mixed feed and is infiltrated 2 hours
Afterwards, then feeding is concentrated.
2, daily management
It feeds by " four is fixed " requirement, daily morning and dusk respectively feed once, and dusk feeding volume is more than morning.The beginning of spring and
Feeding amount is gradually increased and is reduced accordingly during late fall water temperature is gradually increasing and declines, mainly according to fish activity and eat
Depending on food situation.The service condition of bait (or bait) records daily, every the feeding volume of adjustment in 7-10 days, every month to date one
Secondary month feeding amount.
The growing state of a fish is measured at per the end of month according to test requirements document, by size the different each case random samplings of specification
20-25 tail measures body length and weight, and length is accurate to millimeter, and weighing is accurate to 1/10g.Ill fish checks and diagnoses in time, and
Timely medication prevention and treatment.Dead fish is pulled out in time.
To feed feed, the record in time such as water temperature, medicining condition, death condition, after dead fish is pulled out and record of weighing.It is right
The fish of sale carries out weighing record, finally counts survival rate.
Results and discussion:
Table 2 is the correlation data of weight in the breeding process of Larimichthys crocea
As can be seen from the above table, either for small dimension or the rheum officinale fish culture of big specification, survival rate have compared with
Big promotion.It is further directed to Larimichthys crocea immunity and index of correlation, the mechanism of action is further inquired into and needs more tests
Support, will further elucidate in research from now on.
The preparation method of radix pseudostellariae ferment provided by the invention has the advantage that radix pseudostellariae, tealeaves respectively by pre- place
It after reason, does not need to be separated and is directly added into ferment strain and carries out inoculation fermentation processing, reduce processing cost；Subsequent fermentation process can
Small-molecule substance is converted by the macromolecular substances of effective component in radix pseudostellariae, tealeaves, since small-molecule substance is with higher
Pharmacological activity, it is rapid-action, and be easier to be absorbed by animal body, be conducive to animal body and radix pseudostellariae ferment is effectively absorbed
And utilization, to reach good curative effect；Fermentation process can also be by objects such as undesirable substance, taste, peculiar smell in radix pseudostellariae simultaneously
Matter degradation, solves mouthfeel existing for existing radix pseudostellariae, low disadvantage is absorbed and utilized.
The foregoing is merely the preferred embodiment of the present invention, are not intended to restrict the invention, for this field
For technical staff, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any
Modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of preparation method of radix pseudostellariae ferment, which comprises the following steps:
The radix pseudostellariae powder of 5-20 parts of weight is broken to 20-40 mesh by the pretreatment of radix pseudostellariae, and the water of 4~10 times of weight is added 50
~70 degrees Celsius of lower boilings obtain the pretreatment object of radix pseudostellariae；
The tealeaves of 5-20 parts of weight is crushed to 20-40 mesh by the pretreatment of tealeaves, and the water of 4~10 times of weight is added 80~95
Degree Celsius lower boiling obtains the pretreatment object of tealeaves；
The preparation of culture medium, in the fermenter be added radix pseudostellariae, tealeaves pretreatment object, the cane molasses of 80~120 parts of weight,
The tap water of the oligosaccharide of 5~15 parts of weight, 700~1000 parts of weight, keeps the temperature under 90~110 degree, then be down to 30 after mixing
~40 degrees Celsius obtain culture medium；
The saccharomycete of 45~55 parts of weight, 45~55 parts of weight are added into fermentor for inoculation fermentation in culture medium obtained
The lactic acid bacteria of acetic acid bacteria, 45~55 parts of weight is cultivated 40~70 days at 25-35 degrees Celsius, is passed through compressed air and is cultivated, and presses
Contracting air intake is 0.1~0.3vvm, and is spaced predetermined time stirring, stops fermentation after the time, puts tank while stirring,
Sterile sealing is filling, packed products.
2. the preparation method of radix pseudostellariae ferment as described in claim 1, which is characterized in that the tealeaves is white tea, green tea, red
One or more of tea etc..
3. the preparation method of radix pseudostellariae ferment as described in claim 1, which is characterized in that the radix pseudostellariae is 10 parts, described
Tealeaves is 10 parts.
4. the preparation method of radix pseudostellariae ferment as described in claim 1, which is characterized in that the oligosaccharide is wood oligose, sweet
Reveal one or more of oligosaccharides, soyabean oligosaccharides, fructooligosaccharide, synanthrin, galactooligosaccharide etc..
5. the preparation method of radix pseudostellariae ferment as described in claim 1, which is characterized in that the saccharomycete is saccharomyces cerevisiae,
Cloth Lardy yeast, candida tropicalis, the Mixed Microbes of at least two equal proportions in candida krusei etc..
6. the preparation method of radix pseudostellariae ferment as described in claim 1, which is characterized in that the acetic acid bacteria is acetobacter xylinum,
Quasi- acetobacter xylinum, glucose vinegar bacillus, the Mixed Microbes of at least two equal proportions in acetobacter aceti etc..
7. the preparation method of radix pseudostellariae ferment as described in claim 1, which is characterized in that the lactic acid bacteria is acidophilus cream bar
Bacterium, Lactobacillus casei, lactobacillus bulgaricus, the Mixed Microbes of at least two equal proportions in bacillus coagulans etc..
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| CN114190470A (en) * | 2021-10-29 | 2022-03-18 | 厦门海洋职业技术学院 | Novel radix pseudostellariae enzyme, preparation method thereof and application thereof in large yellow croaker culture |
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