CN108813107A - Peanut shell powder biological fermentation feed and preparation method thereof - Google Patents
Peanut shell powder biological fermentation feed and preparation method thereof Download PDFInfo
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- CN108813107A CN108813107A CN201810917275.XA CN201810917275A CN108813107A CN 108813107 A CN108813107 A CN 108813107A CN 201810917275 A CN201810917275 A CN 201810917275A CN 108813107 A CN108813107 A CN 108813107A
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- lactobacillus
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- 235000017060 Arachis glabrata Nutrition 0.000 title claims abstract description 63
- 235000010777 Arachis hypogaea Nutrition 0.000 title claims abstract description 63
- 235000018262 Arachis monticola Nutrition 0.000 title claims abstract description 63
- 235000020232 peanut Nutrition 0.000 title claims abstract description 63
- 239000000843 powder Substances 0.000 title claims abstract description 62
- 238000000855 fermentation Methods 0.000 title claims abstract description 55
- 230000004151 fermentation Effects 0.000 title claims abstract description 51
- 238000002360 preparation method Methods 0.000 title claims abstract description 19
- 241001553178 Arachis glabrata Species 0.000 title 1
- 230000004913 activation Effects 0.000 claims abstract description 73
- 244000105624 Arachis hypogaea Species 0.000 claims abstract description 62
- 241000894006 Bacteria Species 0.000 claims abstract description 48
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 41
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims abstract description 37
- 241000186605 Lactobacillus paracasei Species 0.000 claims abstract description 35
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 35
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 33
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 30
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 30
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 27
- 239000002131 composite material Substances 0.000 claims abstract description 25
- 239000001963 growth medium Substances 0.000 claims description 32
- 239000000243 solution Substances 0.000 claims description 31
- 239000002609 medium Substances 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 12
- 241000186660 Lactobacillus Species 0.000 claims description 9
- 229940039696 lactobacillus Drugs 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 239000002054 inoculum Substances 0.000 claims description 7
- 241000196324 Embryophyta Species 0.000 claims description 6
- 239000006071 cream Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 239000000654 additive Substances 0.000 claims description 5
- 230000000996 additive effect Effects 0.000 claims description 5
- 235000013351 cheese Nutrition 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 claims description 3
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 claims description 3
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 244000025254 Cannabis sativa Species 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 24
- 239000004310 lactic acid Substances 0.000 abstract description 12
- 235000014655 lactic acid Nutrition 0.000 abstract description 12
- 241001465754 Metazoa Species 0.000 abstract description 10
- 239000000835 fiber Substances 0.000 abstract description 7
- 230000000813 microbial effect Effects 0.000 abstract description 7
- 235000019629 palatability Nutrition 0.000 abstract description 5
- 230000000050 nutritive effect Effects 0.000 abstract description 4
- 239000010902 straw Substances 0.000 abstract description 3
- 230000007269 microbial metabolism Effects 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 16
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- 230000007420 reactivation Effects 0.000 description 8
- 230000003213 activating effect Effects 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
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- 235000002017 Zea mays subsp mays Nutrition 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 235000013339 cereals Nutrition 0.000 description 5
- 235000005822 corn Nutrition 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 241000282887 Suidae Species 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000003935 denaturing gradient gel electrophoresis Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000009629 microbiological culture Methods 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000006467 substitution reaction Methods 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000004459 forage Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 229920005610 lignin Polymers 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 229940099596 manganese sulfate Drugs 0.000 description 2
- 239000011702 manganese sulphate Substances 0.000 description 2
- 235000007079 manganese sulphate Nutrition 0.000 description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000009304 pastoral farming Methods 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 2
- 239000001393 triammonium citrate Substances 0.000 description 2
- 235000011046 triammonium citrate Nutrition 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- OBMBUODDCOAJQP-UHFFFAOYSA-N 2-chloro-4-phenylquinoline Chemical compound C=12C=CC=CC2=NC(Cl)=CC=1C1=CC=CC=C1 OBMBUODDCOAJQP-UHFFFAOYSA-N 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 244000199866 Lactobacillus casei Species 0.000 description 1
- 235000013958 Lactobacillus casei Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000007640 basal medium Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 229940036811 bone meal Drugs 0.000 description 1
- 239000002374 bone meal Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000010835 comparative analysis Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000013256 coordination polymer Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000002242 deionisation method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 229940017800 lactobacillus casei Drugs 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- -1 phosphoric acid hydrogen Chemical class 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
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- 239000002699 waste material Substances 0.000 description 1
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- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Fodder In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of peanut shell powder biological fermentation feeds and preparation method thereof, and preparation method includes the following steps：Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus paracasei and bacillus subtilis is taken to be activated to obtain activation lactobacillus plantarum strain, activation Lactobacillus rhamnosus strain, activation Lactobacillus paracasei strain and activation Bacillus subtilis strain respectively；Composite bacteria agent is mixed to get after activation lactobacillus plantarum strain, activation Lactobacillus rhamnosus strain, activation Lactobacillus paracasei strain and activation Bacillus subtilis strain are cultivated respectively；It is mixed after composite bacteria agent is diluted with peanut shell powder, adjusts moisture content and be uniformly mixed, peanut shell powder biological fermentation feed is obtained after fermentation.The present invention is acted on by microbial fermentation, is difficult to be improved stalk palatability by the crude fibre of animal use, improved the nutritive value of feed in decomposing straw.The lactic acid that microbial metabolism generates in fermentation process, can inhibit saprophytic bacteria to grow, and extend the feed storage phase.
Description
Technical field
The present invention relates to field of feed processing, in particular to a kind of peanut shell powder biological fermentation feed and preparation method thereof.
Background technique
In recent years, with the continuous adjustment of China's agricultural structure, China's grain and forage yield are insufficient at present, are formed
The situation of " people and animals strive grain ", demand with Chinese people to animal food quality and quantity, this contradiction is in more prominent
Trend out.Utilize modern biotechnologies and the feeds such as biotechnology, microbial fermentation engineering technology, feed processing technology technology
Nutrition technique means improve the utilization efficiency of storage resources, and developing non-grain fodder resource is to solve feed resource critical shortage, delay
Solve the fundamental solution of national food security.
National Primary Crop Straw total output in 2015 is up to 10.4 hundred million tons, and collecting resource is 9.0 hundred million tons, wherein peanut
Shell yield is up to 5,000,000 tons.These resources can become the good feed of livestock and poultry, " people and animals can be effectively relieved by effective processing
Strive grain " situation.
Peanut shell directly as feed there are the problem of have：Peanut shell crude fiber content is high, and feeding palatability is poor, and feed disappears
Rate is lower.The present invention discloses a kind of method for preparing biological fermentation feed using peanut shell powder, is the utilization of agricultural wastes
New approach is provided, new kind is provided for animal and fowl fodder.
Summary of the invention
In order to solve problems in the prior art, the embodiment of the invention provides a kind of mobile animal breath metabolic determinations to fill
It sets.The technical solution is as follows：
On the one hand, the preparation method of peanut shell powder biological fermentation feed, includes the following steps：
Take lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus rhamnosus (Lactobacillus
Rhamnosus), Lactobacillus paracasei (Lactobacillus paracasei) and bacillus subtilis (Bacillus
Subtilis it) activated to obtain activation lactobacillus plantarum strain, activation Lactobacillus rhamnosus strain respectively, activate secondary cheese cream
Bacillus species and activation Bacillus subtilis strain；
It will activation lactobacillus plantarum strain, activation Lactobacillus rhamnosus strain, activation Lactobacillus paracasei strain and activation
Bacillus subtilis strain is mixed to get composite bacteria agent after being cultivated respectively；
It is mixed after composite bacteria agent is diluted with peanut shell powder, adjusts moisture content and be uniformly mixed, peanut shell is obtained after fermentation
Powder biological fermentation feed.
Further, lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus paracasei and the activation method of bacillus subtilis
For：
Lactobacillus plantarum is taken, is inoculated in MRS culture medium, -36h, picking activate plant to 37 ± 1 DEG C of anaerobism activation cultures for 24 hours
Lactobacillus single colonie is seeded in another MRS culture medium, and 37 ± 1 DEG C of anaerobism activation cultures -36h for 24 hours obtains activation lactobacillus plantarum
Strain；
Lactobacillus rhamnosus is taken, is inoculated in MRS culture medium, -36h, picking activate mouse to 37 ± 1 DEG C of anaerobism activation cultures for 24 hours
Lee's sugar lactobacillus single colonie is seeded in another MRS culture medium, and 37 ± 1 DEG C of anaerobism activation cultures -36h for 24 hours obtains activation rhamnose
Lactobacillus strain；
Lactobacillus paracasei is taken, is inoculated in MRS culture medium, -36h, picking activation are secondary for 24 hours for 37 ± 1 DEG C of anaerobism activation cultures
Lactobacillus casei single colonie is seeded in another MRS culture medium, and 37 ± 1 DEG C of anaerobism activation cultures -36h for 24 hours obtains activating secondary cheese
Lactobacillus strain；
Bacillus subtilis is taken to be inoculated in 37 ± 1 DEG C of LB culture medium aerobic activation culture 12h-24h, picking activates withered grass bud
Spore bacillus single colonie is seeded in another LB culture medium, and 37 ± 1 DEG C of aerobic activation culture 12h-24h obtain activation bacillus subtilis
Bacterium strain.
MRS culture medium composition：1% peptone, 0.5% powdered beef, 0.4% yeast powder, 2% glucose, 0.2% phosphoric acid hydrogen
Dipotassium, 0.5% sodium acetate, 0.2% Triammonium citrate, 0.02% magnesium sulfate, 0.005% manganese sulfate, 0.1% Tween 80；Consolidate
Add 1.5% agar powder when body culture medium；6.2,115 DEG C of high pressure sterilization 30min of pH.
LB culture medium composition：1% tryptone, 0.5% yeast extract, 1%NaCl；Make to add when solid medium
1.5% agar powder；7.0,121 DEG C of high pressure sterilization 30min of pH.
Further, lactobacillus plantarum strain, activation Lactobacillus rhamnosus strain and activation Lactobacillus paracasei bacterium are activated
Kind cultural method be：
Activation lactobacillus plantarum strain, activation Lactobacillus rhamnosus strain, activation Lactobacillus paracasei strain are taken, is connect respectively
Kind in the MRS fluid nutrient medium of three 50ml, 37 ± 1 DEG C of Anaerobic culturels for 24 hours, when surveying each OD for determining bacterium solution under 600nm wavelength
When value is 1, lactobacillus plantarum seed liquor, Lactobacillus rhamnosus seed liquor and Lactobacillus paracasei seed liquor are respectively obtained；
Lactobacillus plantarum seed liquor, Lactobacillus rhamnosus seed liquor and Lactobacillus paracasei seed liquor are taken, with 2% inoculation
Amount is inoculated in respectively in three MRS culture mediums, and 37 ± 1 DEG C of anaerobism expand culture -36h for 24 hours, respectively obtains lactobacillus plantarum bacterium
Liquid, Lactobacillus rhamnosus bacterium solution and Lactobacillus paracasei bacterium solution.
Further, the cultural method for activating Bacillus subtilis strain is：
Activation Bacillus subtilis strain is taken, is inoculated in LB liquid medium, 37 ± 1 DEG C, 150rpm, aerobic culture
12h obtains bacillus subtilis seed liquor when the OD value for measuring bacterium solution under 600nm wavelength is 1；
Bacillus subtilis seed liquor is taken, is inoculated in LB culture medium with 2% inoculum concentration, 37 ± 1 DEG C of aerobic expansion trainings
12h-24h is supported, bacillus subtilis bacterium solution is obtained.
Further, lactobacillus plantarum bacterium solution, Lactobacillus rhamnosus bacterium solution, Lactobacillus paracasei bacterium solution and bacillus subtilis
The mixed proportion of bacterium bacterium solution is 1:1:1:1.
Further, it is mixed after composite bacteria agent being diluted with peanut shell powder, specially：Composite bacteria agent is diluted to OD600=
1,10 liters of composite bacteria agents are added in peanut shell powder per ton.
Further, composite bacteria agent and the mixed fermentation condition of peanut shell powder are normal temperature anaerobic fermentation 30 days.
Further, it is 65% that moisture is adjusted after composite bacteria agent is mixed with peanut shell powder.
Further, composite bacteria agent additive amount is that viable count is greater than 109cfu/g。
On the other hand, a kind of peanut shell powder biological fermentation feed, is prepared using the above method.
Technical solution bring beneficial effect provided in an embodiment of the present invention is：The present invention is acted on by microbial fermentation,
It decomposes and is difficult to improving stalk palatability by the crude fibre (lignin, cellulose, hemicellulose etc.) of animal use in peanut shell
On the basis of, improve the nutritive value of feed.The lactic acid that microbial metabolism generates in fermentation process can inhibit saprophytic bacteria raw
It is long, extend the feed storage phase.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other
Attached drawing.
Fig. 1 is the PCR-DGGE map of microbiology turbidity in peanut shell powder fermentation process of the present invention.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with attached drawing to embodiment party of the present invention
Formula is described in further detail.
One, the preparation of composite bacteria agent
(1) prepared by culture medium
Lactic acid bacteria culture medium (MRS culture medium)：
Take peptone 10.0g, powdered beef 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, phosphoric acid hydrogen two
Potassium 2.0g, sodium acetate 5.0g, Triammonium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g and agar powder 15.0g add deionization
Water is settled to 1.0L, dissolves by heating, and adjusts pH value to 6.2,115 DEG C of high pressure sterilization 30min.When needing solid medium, upper
State the agar powder of addition 1.5% on the basis of forming.
Bacterium basal medium (LB culture medium)
Tryptone 10.0g, yeast extract 5.0g, NaCl 10.0g are taken, adds deionized water dissolving, with 5.0mol/L's
NaOH adjusts pH value to 7.0,1.0L is settled to deionized water, in 121 DEG C of steam sterilizing 30min.When needing solid medium,
The agar powder of addition 1.5% on the basis of above-mentioned composition.
(2) actication of culture
Each strain purchase is from Research for Industrial Microbial Germ preservation administrative center and China General Microbiological culture presevation management
The heart, specific ordering information is referring to Tables 1 and 2.
1 Chinese industrial Microbiological Culture Collection administrative center ordering information of table
2 China General Microbiological culture presevation administrative center ordering information of table
Take lactobacillus plantarum preservation of bacteria strain MRS solid plate culture medium cross, 37 ± 1 DEG C of anaerobism activation cultures for 24 hours-
36h obtains once activating lactobacillus plantarum strain.Picking one primary activation single colonie, in a new MRS solid plate training
It supports and crosses in base, 37 ± 1 DEG C of anaerobism activation cultures -36h for 24 hours obtains re-activation lactobacillus plantarum strain.
Take Lactobacillus rhamnosus preservation of bacteria strain MRS solid plate culture medium cross, 37 ± 1 DEG C of anaerobism activation cultures for 24 hours-
36h obtains once activating Lactobacillus rhamnosus strain.Picking one primary activation single colonie, in a new MRS solid plate
It crosses in culture medium, 37 ± 1 DEG C of anaerobism activation cultures -36h for 24 hours obtains re-activation Lactobacillus rhamnosus strain.
Take Lactobacillus paracasei preservation of bacteria strain MRS solid plate culture medium cross, 37 ± 1 DEG C of anaerobism activation cultures for 24 hours-
36h obtains once activating Lactobacillus paracasei strain.Picking one primary activation single colonie, in a new MRS solid plate
It crosses in culture medium, 37 ± 1 DEG C of anaerobism activation cultures -36h for 24 hours obtains re-activation Lactobacillus paracasei strain.
Bacillus subtilis preservation of bacteria strain is taken to cross in LB solid plate culture medium, 37 ± 1 DEG C of aerobic activation culture 12h-
For 24 hours, it obtains once activating Bacillus subtilis strain.Picking one primary activation bacillus subtilis single colonie is new at one
LB solid plate culture medium in cross, 37 ± 1 DEG C of aerobic activation culture 12h-24h obtain re-activation bacillus subtilis
Strain.
(3) expand culture
One re-activation lactobacillus plantarum single colonie of picking, is inoculated in 50mL MRS fluid nutrient medium, 37 ± 1 DEG C are detested
Oxygen expands culture for 24 hours, OD600It is 1.0 or so, obtains lactobacillus plantarum seed liquor.
One re-activation Lactobacillus rhamnosus single colonie of picking, is inoculated in 50mL MRS fluid nutrient medium, 37 ± 1 DEG C
Anaerobism expands culture for 24 hours, OD600It is 1.0 or so, obtains Lactobacillus rhamnosus seed liquor.
One re-activation Lactobacillus paracasei single colonie of picking, is inoculated in 50mL MRS fluid nutrient medium, 37 ± 1 DEG C
Anaerobism expands culture for 24 hours, OD600It is 1.0 or so, obtains Lactobacillus paracasei seed liquor.
One re-activation bacillus subtilis single colonie of picking, is inoculated in 50mL LB liquid medium, 37 ± 1 DEG C,
12h, OD are cultivated in 150rpm, aerobic expansion600It is 1 or so, obtains bacillus subtilis seed liquor.
(4) composite bacteria agent is prepared
Lactobacillus plantarum seed liquor is taken, is inoculated into MRS fluid nutrient medium with 2% inoculum concentration, 37 ± 1 DEG C of anaerobism expand training
- 36h for 24 hours is supported, lactobacillus plantarum bacterium solution is obtained.
Lactobacillus rhamnosus seed liquor is taken, is inoculated into MRS fluid nutrient medium with 2% inoculum concentration, 37 ± 1 DEG C of anaerobism expand
- 36h for 24 hours is cultivated, Lactobacillus rhamnosus bacterium solution is obtained.
Lactobacillus paracasei seed liquor is taken, is inoculated into MRS fluid nutrient medium with 2% inoculum concentration, 37 ± 1 DEG C of anaerobism expand
- 36h for 24 hours is cultivated, Lactobacillus paracasei bacterium solution is obtained.
Bacillus subtilis seed liquor is taken, is inoculated into LB liquid medium with 2% inoculum concentration, 37 ± 1 DEG C of aerobic expansions
12h-24h is cultivated, bacillus subtilis bacterium solution is obtained.
Lactobacillus plantarum bacterium solution, Lactobacillus rhamnosus bacterium solution, Lactobacillus paracasei bacterium solution and bacillus subtilis bacterium solution are taken,
By volume 1:1:1:1 ratio is uniformly mixed, and obtains composite bacteria agent.
Two, the preparation of peanut shell powder fermented feed
(1) preparation of peanut shell powder
The peanut shell not gone mouldy is taken, precomminution is carried out through gear grinder, is then crushed again through beater grinder.It crushes
Machine sieve diameter is 1 millimeter.Smashed peanut shell powder all crosses 40 mesh screens.
(2) composite bacteria agent dilutes
The material-water ratio of peanut shell powder and water is (w/v)：3.5:6.5, i.e., every 350 kilograms of peanut shell powders need plus water 650 is public
Jin.Composite bacteria agent inoculum concentration is greater than 109cfu/g.Composite bacteria agent is adjusted to 6 × 10 in advance in 650 kg of water5cfu/ml
Water.
(3) mixing pack fermentation
350 kilograms of peanut shell powders are taken, 650 kilograms of composite bacteria agent dilutions are mixed by batch mixer, are then dispensed
Into anaerobic fermentation bag, per packed 50 kilograms, stacks, ferment after sealing.Anaerobic fermentation 30 days is at normal temperature to get to flower
Raw shell powder biological fermentation feed.
Three, growing and fattening pigs feeding effect is tested
(1) subjects and place：Selecting 120 healthy Songliao Black Pig Heart Fatties is subjects, weight at 45-50 kilograms, with
Machine is divided into four groups, i.e. control group (feeding fattening feed), and test group (is mixed into peanut shell powder fermented feed, root in fattening feed
According to the difference of mixed peanut shell powder fermented feed amount, this test group is further divided into three groups), every group of 30 pigs；This experiment is in Jilin
Herding research institute of herding science branch of Shanxi Academy of Agricultural Sciences carries out.
(2) fattening feed forms：It is shown in Table 3.
3 fattening pannage of table matches table
| Serial number | Composition | Ratio (%) |
| 1 | Corn | 63.0 |
| 2 | Dregs of beans | 7.5 |
| 3 | Corn protein powder | 9.0 |
| 4 | Wheat bran | 15.0 |
| 5 | Fish meal | 2.0 |
| 6 | Bone meal | 0.5 |
| 7 | Mountain flour | 1.0 |
| 8 | Salt | 0.5 |
| 9 | Premix | 1.5 |
(3) test method and time：
Each group test is shown in Table 4 with feed formula；By normal feeding, freely look for food；Experiment periods are 35 days.
Test index：Record every group of feed intake, body weight evolution, excrement situation.Calculate average daily gain, average day feeding
Amount and feed conversion rate.
Average daily gain：Respectively at first day and test morning last day of formal test, weigh on an empty stomach by head, by end
The difference of weight and starting weight calculates the average daily gain of each group pig divided by experiment number of days.
Average daily gain：Record each group inventory and surplus doses, inventory subtract surplus doses again divided by experiment number of days,
Calculate average daily gain.
Feed conversion rate：There emerged a each group divided by the average daily gain of each group pig with the average daily gain of each group pig
Pannage conversion ratio.
The observation of excrement situation：Whether normal observe excrement, such as：Tangible (cylinder), it is tangible (soft stool), it is shapeless (sticky)
Deng.
Each test group feed of table 4 forms table
(4) result
1, peanut shell powder fermented feed analyzes result
Peanut shell powder fermentation front and back Related Component analysis comparing result is shown in Table 5.The pH of raw material is 4.9, after fermentation before fermenting
It is 3.54；In addition, lactic acid content is greater than 100mg/g fermented product after fermentation without detection lactic acid before fermentation.The pH of fermented feed
It is lower, lactic acid content is higher, be conducive to feed long-term preservation.
The present invention is acted on by microbial fermentation, is difficult in decomposing straw by crude fibre (lignin, the fiber of animal use
Element, hemicellulose etc.), on the basis of improving stalk palatability, improve the nutritive value of feed.Microorganism generation in fermentation process
It thanks to the lactic acid of generation, saprophytic bacteria can be inhibited to grow, extend the feed storage phase.
After fermentation compared with before fermentation, dry matter, crude protein are improved, and the content of water soluble carbohydrates is obvious
It reduces, illustrates probiotics using carbohydrate as the nutriment of thallus breeding and lactic acid metabolism.Lactic acid bacteria after fermentation
Content obviously increases, these probiotics have facilitation for adjusting animal intestine stable state.We also by PCR-DGGE side
Method has detected microbial diversity in fermentation process and changes, as shown in Figure 1, A is the map of PCR-DGGE, wherein 0 represents inoculation
Microorganism situation in preceding peanut shell powder, 10,20,30 points of expressions are fermented the 10th day, 20 days and 30 days；B is analyzed using software
Figure A in the bin number that contains, wherein meaning represented by 0,10,20,30 is the same as scheming A.It can visually see from figure B, with
The extension of fermentation time, microbial diversity reduce, in conjunction with 5 kinds of table detect as a result, after peanut shell powder inoculating compound bacterium agent
Fermentation 30 days, the microorganism in product concentrates on lactic acid bacteria, and most microorganisms in raw material are effectively suppressed.
5 peanut shell powder of table fermentation front and back Related Component comparative analysis result
| Ingredient | Material content before fermenting | Product content after fermentation |
| PH value | 4.9±0.03 | 3.54±0.03 |
| Dry matter (DM, %) | 36.84±0.00 | 37.73±0.03 |
| Crude protein (CP, %DM) | 8.45±0.28 | 8.74±1.41 |
| Ammoniacal nitrogen/total nitrogen | 5.18±0.28 | 5.92±0.36 |
| Water soluble carbohydrates (WSC, mg/g) | 24.00±0.04 | 9.72±0.49 |
| Neutral detergent fiber (NDF, %DM) | 65.03±0.74 | 61.61±3.29 |
| Acid detergent fiber (ADF, %DM) | 43.03±0.83 | 55.91±0.47 |
| Lactic acid bacteria (cfu/g) | 1.00×106 | 1.95×1010 |
| Saccharomycete (cfu/g) | 1.00×103 | 4.79×103 |
| Lactic acid (mg/g) | - | 103.25±0.11 |
| Acetic acid (mg/g) | - | 2.43±0.69 |
2, feeding experiment result
Influence of the 6 peanut shell powder fermented feed Different adding amount of table to growing and fattening pigs
| Group | Control group | Test group 1 | Test group 2 | Test group 3 |
| Head number (head) | 30 | 30 | 30 | 30 |
| Start average weight (kg) | 50.61±0.21 | 49.56±0.12 | 49.80±0.17 | 50.60±0.15 |
| Last average weight (kg) | 79.17±1.21 | 79.52±0.51 | 80.11±0.28 | 80.21±0.50 |
| Average daily gain (kg/) | 0.816±0.04 | 0.856±0.01 | 0.866±0.01 | 0.846±0.02 |
| Average day consumption feed (kg/) | 2.342 | 2.337 | 2.33 | 2.377 |
| Feedstuff-meat ratio | 2.87 | 2.73 | 2.69 | 2.81 |
In table 6, test group 1, the additive amount of peanut shell powder fermented feed is 6.3%, is equivalent to the 10% of substitution corn；Examination
Group 2 is tested, the additive amount of peanut shell powder fermented feed is 12.6%, is equivalent to the 20% of substitution corn；Test group 3, peanut shell powder
The additive amount of fermented feed is 18.6%, is equivalent to the 30% of substitution corn；
The feeding of each group fattening pannage works well, and single grazing rate is high, the statistical result discovery after feeding 35 days, addition
Average day consumption forage volume of the test group of peanut shell powder fermented feed compared with control group compared to every pig is not much different, overall feedstuff-meat ratio
It is low compared with control group, the wherein significant effect of test group 2, test group 3 and control group no significant difference.
Excrement situation discovery from daily, is added to the test group of peanut shell powder fermented feed, formed stools are good,
It is fine and smooth and not hard；Excrement amount is reduced compared with control group, and without significantly stink and ammonia taste.
The above results show that the present invention works well in growing and fattening pigs feeding experiment, can substitute the jade in complete feed
Rice, and can be improved the grazing rate of animal, reduce feedstuff-meat ratio, improve environment.Illustrate that the present invention is improving immunity of livestock, disease-resistant
There is remarkable effect in terms of power, growth promotion, increases culture efficiency.
The beneficial effects of the present invention are：
The present invention provides a kind of peanut shell powder biological fermentation feeds and preparation method thereof, and advantage is as follows：
1, at low cost, fermented feed section grain, environmental protection are prepared using peanut shell powder；It is main hair with agricultural by-products peanut shell
Ferment raw material need to only crush peanut shell it is not necessary that other auxiliary material processing are added.
2, consume energy low, small investment, pollution-free；Strain used in the present invention is easily cultivated；Agricultural by-products peanut is utilized in fermentation
Shell reduces peanut shell bring environmental problem；There is no the discharge of waste water, waste residue in fermentation process, reduces environmental pollution.
3, the palatability for improving feed, improves the nutritive value of feed；Contain plant cream bar in the present invention in composite bacteria agent
Bacterium, Lactobacillus rhamnosus, Lactobacillus paracasei and bacillus subtilis, four kinds of microbial inoculums are that feed addictive allows the micro- of addition
Biology, fermented feed quality safety.Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus paracasei can produce greatly in fermentation
Organic acid is measured, feed flavor is improved；Bacillus subtilis, which can be metabolized, generates a variety of digestive ferments, is not digested in decomposition peanut
Cellulose improves efficiency of feed utilization；Bacillus subtilis can synthesize multivitamin simultaneously, increase feed nutrition.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. the preparation method of peanut shell powder biological fermentation feed, which is characterized in that include the following steps：
Take lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus rhamnosus (Lactobacillus
Rhamnosus), Lactobacillus paracasei (Lactobacillus paracasei) and bacillus subtilis (Bacillus
Subtilis it) activated to obtain activation lactobacillus plantarum strain, activation Lactobacillus rhamnosus strain respectively, activate secondary cheese cream
Bacillus species and activation Bacillus subtilis strain；
By the activation lactobacillus plantarum strain, the activation Lactobacillus rhamnosus strain, the activation Lactobacillus paracasei bacterium
Kind and the activation Bacillus subtilis strain are mixed to get composite bacteria agent after being cultivated respectively；
It will be mixed after composite bacteria agent dilution with peanut shell powder, adjust moisture content and be uniformly mixed, peanut shell is obtained after fermentation
Powder biological fermentation feed.
2. the preparation method of peanut shell powder biological fermentation feed as described in claim 1, which is characterized in that the plant cream bar
Bacterium, Lactobacillus rhamnosus, Lactobacillus paracasei and bacillus subtilis activation method be：
Lactobacillus plantarum is taken, is inoculated in MRS culture medium, -36h, picking activate plant cream bar to 37 ± 1 DEG C of anaerobism activation cultures for 24 hours
Bacterium single colonie is seeded in another MRS culture medium, and 37 ± 1 DEG C of anaerobism activation cultures -36h for 24 hours obtains the activation lactobacillus plantarum
Strain；
Lactobacillus rhamnosus is taken, is inoculated in MRS culture medium, -36h, picking activate rhamnose to 37 ± 1 DEG C of anaerobism activation cultures for 24 hours
Lactobacillus single colonie is seeded in another MRS culture medium, and 37 ± 1 DEG C of anaerobism activation cultures -36h for 24 hours obtains the activation rhamnose
Lactobacillus strain；
Lactobacillus paracasei is taken, is inoculated in MRS culture medium, -36h, picking activate secondary cheese to 37 ± 1 DEG C of anaerobism activation cultures for 24 hours
Lactobacillus single colonie is seeded in another MRS culture medium, and 37 ± 1 DEG C of anaerobism activation cultures -36h for 24 hours obtains the secondary cheese of the activation
Lactobacillus strain；
Bacillus subtilis is taken to be inoculated in 37 ± 1 DEG C of LB culture medium aerobic activation culture 12h-24h, picking activates bacillus subtilis
Bacterium single colonie is seeded in another LB culture medium, and 37 ± 1 DEG C of aerobic activation culture 12h-24h obtain the activation bacillus subtilis
Bacterium strain.
3. the preparation method of peanut shell powder biological fermentation feed as claimed in claim 2, which is characterized in that the activation plant
Lactobacillus strain, activation Lactobacillus rhamnosus strain and activate Lactobacillus paracasei strain cultural method be：
Activation lactobacillus plantarum strain, activation Lactobacillus rhamnosus strain, activation Lactobacillus paracasei strain are taken, is inoculated in respectively
In three MRS fluid nutrient mediums, 37 ± 1 DEG C of Anaerobic culturels for 24 hours, when surveying each OD value for determining bacterium solution under 600nm wavelength is 1, divide
Lactobacillus plantarum seed liquor, Lactobacillus rhamnosus seed liquor and Lactobacillus paracasei seed liquor are not obtained；
The lactobacillus plantarum seed liquor, Lactobacillus rhamnosus seed liquor and Lactobacillus paracasei seed liquor are taken, with 2% inoculation
Amount is inoculated in respectively in three MRS culture mediums, and 37 ± 1 DEG C of anaerobism expand culture -36h for 24 hours, respectively obtains lactobacillus plantarum bacterium
Liquid, Lactobacillus rhamnosus bacterium solution and Lactobacillus paracasei bacterium solution.
4. the preparation method of peanut shell powder biological fermentation feed as claimed in claim 3, which is characterized in that the activation withered grass
The cultural method of Bacillus is：
Activation Bacillus subtilis strain is taken, is inoculated in LB liquid medium, 37 ± 1 DEG C, 150rpm, aerobic culture 12h, when
When the OD value for measuring bacterium solution under 600nm wavelength is 1, bacillus subtilis seed liquor is obtained；
Bacillus subtilis seed liquor is taken, is inoculated in LB culture medium with 2% inoculum concentration, 37 ± 1 DEG C of aerobic expansion cultures
12h-24h obtains bacillus subtilis bacterium solution.
5. the preparation method of peanut shell powder biological fermentation feed as claimed in claim 4, which is characterized in that the plant cream bar
Bacterium bacterium solution, Lactobacillus rhamnosus bacterium solution, the mixed proportion of Lactobacillus paracasei bacterium solution and bacillus subtilis bacterium solution are 1:1:1:
1。
6. the preparation method of peanut shell powder biological fermentation feed as claimed in claim 5, which is characterized in that by the compound bacteria
It is mixed after dilution agent with peanut shell powder, specially：The composite bacteria agent is diluted to OD600=1, in the peanut shell powder per ton
10 liters of composite bacteria agents are added.
7. the preparation method of peanut shell powder biological fermentation feed as claimed in claim 6, which is characterized in that the composite bacteria agent
It is normal temperature anaerobic fermentation 30 days with the mixed fermentation condition of peanut shell powder.
8. the preparation method of peanut shell powder biological fermentation feed as claimed in claim 7, which is characterized in that the composite bacteria agent
It is 65% that moisture is adjusted after mixing with peanut shell powder.
9. the preparation method of peanut shell powder biological fermentation feed as claimed in claim 8, which is characterized in that the composite bacteria agent
Additive amount is that viable count is greater than 109cfu/g。
10. a kind of peanut shell powder biological fermentation feed, which is characterized in that be prepared using the method for claim 1-9.
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