Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of have improve buck immunity, the rapid recovery breeding is tired, the physiological regulation agent that strengthen breeding capacity, delays senility has instant effect, the characteristics such as safe and reliable.
Another object of the present invention is to provide the preparation method of this physiological regulator for male animals.
The present invention also provides a kind of feed that comprises this physiological regulator for male animals.
To achieve these goals, the technical solution adopted in the present invention is as follows:
The invention provides a kind of physiological regulator for male animals, it is characterized in that probio is inoculated in the culture medium that contains Chinese medicine and ferments and be prepared from.
Described a kind of physiological regulator for male animals is characterized in that described Chinese medicine comprises following component and mass fraction:
1~10 part of barrenwort, 1~10 part of the bark of eucommia, 1~10 part, Radix Glycyrrhizae, 1~10 part of radix rehmanniae recen.
Described a kind of physiological regulator for male animals, is characterized in that described probio comprises one or more of saccharomycete, lactic acid bacteria and/or Bacillus.
Described a kind of physiological regulator for male animals, is characterized in that one or more in the Poria cocos of dandelion that described Chinese medicine also comprises that mass fraction is 1~10 part, the cynomorium songaricum of 1~5 part, the Radix Astragali of 1~10 part, the dried orange peel of 2~5 parts, the seed of Chinese dodder of 1~6 part, the Radix Codonopsis of 2~4 parts, 1~5 portion of flos caryophylli, the Morinda officinalis of 1~4 part, the bark of official magnolia of 2~6 parts, the spina date seed of 1~5 part, the Chinese yam of 1~10 part and 1~5 part.
Described a kind of physiological regulator for male animals, is characterized in that described yeast is candida utili, saccharomyces cerevisiae and/or brewer's yeast.
Described a kind of physiological regulator for male animals, is characterized in that described lactic acid bacteria comprises lactobacillus lactis, lactobacillus acidophilus, lactobacillus thermophilus, Lactobacillus casei, lactobacillus lactis, Lactobacillus plantarum and/or lactobacillus bulgaricus.
Described a kind of mother animal physiological regulation agent, is characterized in that described Bacillus comprises bacillus subtilis and/or bacillus licheniformis.
The preparation method of described a kind of physiological regulator for male animals is characterized in that comprising the following steps:
(1) Chinese medicine pretreatment
Choose Chinese medicine, under 50~60 ℃, dry 2~3h; Chinese medicine after drying is pulverized, crossed 80~120 mesh sieves;
(2) prepare strain fermentating liquid
Each bacterial classification is inoculated in respectively in optimum medium separately, cultivate under suitable condition primary seed solution, be transferred to respectively in the secondary seed fermentation tank during to exponential phase and enlarge cultivation when each bacteria growing, stop while reaching exponential phase cultivating, standby;
(3) herb fermenting
Chinese medicine after pulverizing and sieving is mixed according to a certain ratio, pack in fermentation tank, add the dregs of beans that accounts for traditional Chinese medicine quality 0~5%, 5~15% corn flour, 0~10% wheat bran, add water to water content between 50~70%, after soaking 12~24h, regulate between pH value 6.0~7.5, at 121 ℃ of lower steam sterilizing 15~20min, cooling after, add 5%~15% the total strain liquid that accounts for the fermentate gross mass, 28~37 ℃ ferment 24~72 hours, every 8h, stir once; After fermentation ends, under 35~50 ℃, dry, obtain physiological regulator for male animals.
The preparation method of described physiological regulator for male animals, is characterized in that total strain liquid is the seed mixture liquid that the secondary seed solution by each bacterium is mixed by equal proportion.
Described a kind of feed containing physiological regulator for male animals, is characterized in that, comprises the constituent of following mass ratio: described physiological regulator for male animals 0.02~0.2% and auxiliary material 99.98~99.8%.
The invention provides a kind of physiological regulator for male animals and preparation method thereof, have the following advantages:
1, physiological regulator for male animals of the present invention has the immunity that improves buck, strengthen the reproduction of male animal function, improve spermatozoon activity, conception rate, obviously extend and promote its breeding time and quality, rapid recovery breeding is tired, and the effect such as delay senility;
2, physiological regulator for male animals of the present invention is a kind of novel fodder additive of the interaction development that adopts probio and Chinese medicine, have have no side effect, the characteristics such as pollution-free, drug residue free; Ensure livestock products safety, be easy to people's health, increase fanning economics simultaneously;
3, physiological regulator for male animals of the present invention is without the species specificity, and effect stability is reliable, and applicable animal range is wide.
, it should be understood that described embodiment is only for the present invention is described, rather than limit the scope of the invention by any way more specific description the present invention by following examples.
The specific embodiment
Embodiment 1
Chinese medicine: 6 parts of barrenwort, 8 parts, Radix Glycyrrhizae, 8 parts of the barks of eucommia, 5 parts of radix rehmanniae recens
The preparation method:
(1) S. cervisiae is inoculated in malt extract medium, 28 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution, when bacterial content reaches 10
7-8during the CFU/ml left and right, primary seed solution is inoculated into inoculum concentration 4% in the secondary seed fermentation tank that nutrient broth is housed and enlarges and cultivate, cultivation temperature is 30 ℃, and rotating speed is 180rpm, when amount of yeast reaches 10
9during the CFU/ml left and right, stop fermentation, standby;
(2) choose above-mentioned each Chinese medicine, under 50~60 ℃, dry 2~3h; Chinese medicine after drying is pulverized, crossed the 80-120 mesh sieve, standby;
(3) take the herb powder after pulverizing by above-mentioned formula, add the dregs of beans that accounts for traditional Chinese medicine quality 1%, 5% corn flour, 5% wheat bran, add water to water content 60%, mix, at room temperature soak 24h, regulate the pH7.0 left and right, at 121 ℃ of lower steam sterilizing 15min, cooling after, add 5% the S. cervisiae bacterium liquid that accounts for the fermentate gross mass, at 30 ℃ of fermentation 48h, every 8h, stir once; When total viable count reaches 1.0 * 10
9stop fermentation during CFU/ml, 45 ℃ of lower vacuum drying, make physiological regulator for male animals.
Embodiment 2
Chinese medicine: 5 parts of barrenwort, 3 parts, Radix Glycyrrhizae, 5 parts of the barks of eucommia, 2 parts of radix rehmanniae recens, 5 parts of the Radixs Astragali, 2 parts of cynomorium songaricums, 1 part of the seed of Chinese dodder
The preparation method:
(1) bacillus subtilis is inoculated in common nutrient broth medium, at 37 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution, when bacterial content reaches 10
7-8during the CFU/ml left and right, in the secondary seed fermentation tank that primary seed solution is inoculated into inoculum concentration 3%, enlarge and cultivate, cultivation temperature is 37 ℃, and rotating speed is 180rpm, when Bacillus content reaches 10
9during the CFU/ml left and right, stop fermentation, standby;
(2) Lactobacillus plantarum is inoculated in the MRS culture medium, 37 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution, when bacterial content reaches 10
7-8during the CFU/ml left and right, primary seed solution is inoculated into inoculum concentration 5% in the secondary seed fermentation tank that nutrient broth is housed and enlarges and cultivate, cultivation temperature is 37 ℃, and rotating speed is 180rpm, when lactic acid bacteria content reaches 10
9during the CFU/ml left and right, stop fermentation, standby;
(3) choose above-mentioned each Chinese medicine, under 50~60 ℃, dry 2~3h; Chinese medicine after drying is pulverized, crossed the 80-120 mesh sieve, standby;
(4) take the herb powder after pulverizing by above-mentioned formula, add the dregs of beans that accounts for traditional Chinese medicine quality 3%, 8% corn flour, 4% wheat bran, add water to water content 55%, mix, at room temperature soak 24h, regulate the pH6.5 left and right, at 121 ℃ of lower steam sterilizing 20min, cooling after, add respectively the bacillus subtilis bacterium liquid that accounts for fermentate gross mass 4% and 4% Lactobacillus plantarum bacterium liquid, at 37 ℃ of fermentation 36h, every 8h, stir once; When total viable count reaches 1.0 * 10
9stop fermentation during CFU/ml, 40 ℃ of lower vacuum drying, make physiological regulator for male animals.
Embodiment 3
Chinese medicine: 4 parts of barrenwort, 3 parts, Radix Glycyrrhizae, 6 parts of the barks of eucommia, 3 parts of radix rehmanniae recens, 2 parts of cynomorium songaricums, 1 part of the bark of official magnolia, 1 part of spina date seed, 2 parts of the Radixs Astragali
The preparation method:
(1) candida utili, brewer's yeast and saccharomyces cerevisiae are inoculated into respectively in malt extract medium, 28~30 ℃, the 220rpm shaking table is cultivated, and the production primary seed solution, when bacterial content reaches 10
7-8during the CFU/ml left and right, in the secondary seed fermentation tank that primary seed solution is inoculated into inoculum concentration 7%, enlarge and cultivate, cultivation temperature is 28~30 ℃, and rotating speed is 220rpm, when amount of yeast reaches 10
9during the CFU/ml left and right, stop fermentation, standby;
(2) choose above-mentioned each Chinese medicine, under 50~60 ℃, dry 2~3h; Chinese medicine after drying is pulverized, crossed the 80-120 mesh sieve, standby;
(3) take the herb powder after pulverizing by above-mentioned formula, add the dregs of beans that accounts for traditional Chinese medicine quality 2%, 6% corn flour, 10% wheat bran, add water to water content 60%, mix, at room temperature soak 24h, regulate the pH6.9 left and right, at 121 ℃ of lower steam sterilizing 20min, cooling after, add respectively the candida utili bacterium bacterium liquid that accounts for fermentate gross mass 2%, 2% saccharomyces cerevisiae bacterium liquid and 2% S. cervisiae bacterium liquid, at 28~30 ℃ of fermentation 48h, every 8h, stir once; When total viable count reaches 1.0 * 10
9stop fermentation during CFU/ml, 50 ℃ of lower vacuum drying, make physiological regulator for male animals.
Embodiment 4
Chinese medicine: 3 parts of barrenwort, 3 parts, Radix Glycyrrhizae, 6 parts of the barks of eucommia, 2 parts of dried orange peels, 1 part of the Radix Astragali, 1 part of Radix Codonopsis, 1 part of flos caryophylli, 2 parts of Chinese yams, 1 part of Morinda officinalis, 2 parts, Poria cocos
The preparation method:
(1) bacillus licheniformis and bacillus subtilis are inoculated into respectively in common nutrient broth medium, at 37 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution, when bacterial content reaches 10
7-8during the CFU/ml left and right, in the secondary seed fermentation tank that primary seed solution is inoculated into inoculum concentration 3%, enlarge and cultivate, cultivation temperature is 34.5 ℃, and rotating speed is 180rpm, when Bacillus content reaches 10
9during the CFU/ml left and right, stop fermentation, standby;
(2) saccharomyces cerevisiae and product candida colliculosa are inoculated into respectively in malt extract medium, 28 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution, when bacterial content reaches 10
7-8during the CFU/ml left and right, in the secondary seed fermentation tank that primary seed solution is inoculated into inoculum concentration 4.5%, enlarge and cultivate, cultivation temperature is 34.5 ℃, and rotating speed is 180rpm, when amount of yeast reaches 10
9during the CFU/ml left and right, stop fermentation, standby;
(3) lactobacillus thermophilus and Lactobacillus casei are inoculated into respectively in the MRS culture medium, 37 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution, when bacterial content reaches 10
7-8during the CFU/ml left and right, primary seed solution is inoculated into inoculum concentration 4.5% in the secondary seed fermentation tank that nutrient broth is housed and enlarges and cultivate, cultivation temperature is 34.5 ℃, and rotating speed is 180rpm, when lactic acid bacteria content reaches 10
9during the CFU/ml left and right, stop fermentation, standby;
(4) choose above-mentioned each Chinese medicine, under 50~60 ℃, dry 2~3h; Chinese medicine after drying is pulverized, crossed the 80-120 mesh sieve, standby;
(5) take the herb powder after pulverizing by above-mentioned formula, add the dregs of beans that accounts for traditional Chinese medicine quality 4%, 10% corn flour, 5% wheat bran, add water to water content 52%, mix, at room temperature soak 24h, regulate the pH6.9 left and right, at 121 ℃ of lower steam sterilizing 18min, after cooling, add respectively the Bacillus licheniformis liquid that accounts for fermentate gross mass 2%, 2% bacillus subtilis bacterium liquid, 2% candida utili bacterium bacterium liquid, 2% S. cervisiae bacterium liquid, 2% lactobacillus thermophilus bacterium liquid and 2% Lactobacillus casei bacterium liquid, at 34.5 ℃ of fermentation 36h, every 8h, stir once, when total number of bacteria reaches 1.0 * 10
9stop fermentation during CFU/ml, 50 ℃ of lower vacuum drying, make physiological regulator for male animals.
Embodiment 5
A kind of feed containing physiological regulator for male animals (bull is used) comprises the constituent of following mass percent: physiological regulator for male animals 0.1% and auxiliary material 99.9%.In 1 ton of feed, the addition of physiological regulator for male animals is 1000g.Feed can specifically comprise the constituent of following mass fraction: press 0.1 part of the prepared physiological regulator for male animals of the embodiment of the present invention 2,60 parts of corns, 16.7 parts of wheat brans, 18 parts of Soybean Meaies, 1.5 parts of stone flours, 1.2 parts of calcium monohydrogen phosphates, 1 part of salt, 0.5 part, sodium bicarbonate, 1% 1 part of breeding oxen premix.
Embodiment 6
A kind of feed containing physiological regulator for male animals (boar is used) comprises the constituent of following mass percent: physiological regulator for male animals 0.06% and auxiliary material 99.94%.In 1 ton of feed, the addition of physiological regulator for male animals is 600g.Feed can specifically comprise the constituent of following mass fraction: press 0.06 part of the prepared physiological regulator for male animals of the embodiment of the present invention 3,65 parts of corns, 17.94 parts of wheat brans, 12 parts of Soybean Meaies, 1 part of fish meal, 1.3 parts of stone flours, 1.3 parts of calcium monohydrogen phosphates, 0.4 part of salt, 1% 1 part of herd boar premix.
Below by concrete test example, the beneficial effect of physiological regulator for male animals of the present invention is described.
Test example 1
This test example has been studied the impact of the physiological regulator for male animals of the use embodiment of the present invention 1 preparation on quantity and the quality of herd boar sperm.
Test method: 10 of the public boars of Du Luoke of selecting 270 age in days left and right to be in a good state of health, be divided at random two groups, control group fed does not add the basal diet of any medicine, and test group is fed and add the physiological regulator for male animals 500g of embodiment 1 preparation in basal diet per ton; Feed 30 days continuously, semen collection in every 10 days once, is measured semen volume, sperm concentration, sperm motility rate, Acrosome abnormity rate and the rate of teratosperm of each boar.Result of the test is in Table 1.
The affect * of table 1 on herd boar sperm, sperm concentration, sperm motility rate, Acrosome abnormity rate and rate of teratosperm
*letter
amean P<0.01, compare with control group, difference is extremely remarkable; Letter
bmean P<0.05, with control group, compare significant difference.
Result of the test shows: ejaculate volume, sperm concentration that the test group herd boar is added after physiological regulator for male animals the 20th day and the 30th day all significantly increase, and sperm motility rate obviously improves, and with control group, compares, and difference is remarkable (P<0.01) extremely.After test group is added physiological regulator for male animals, rate of teratosperm and Acrosome abnormity rate all obviously reduce, and after using, within the 20th day, with the rate of teratosperm of the 30th day, compare significant difference (P<0.05) with control group with Acrosome abnormity rate.Illustrate that physiological regulator for male animals of the present invention can increase the quantity of sperm and improve the quality of sperm, thereby promote its fecundity.
Test example 2
This test example has been studied the impact of the physiological regulator for male animals of the use embodiment of the present invention 4 preparations on quantity and the quality of breeding oxen sperm.
Test method: certain pasture provides 10 adult Holstein bulls that age in days is close, is divided at random two groups, 5 every group, is respectively test group and control group.The feed daily ration of the physiological regulator for male animals that adds the embodiment of the present invention 4 preparations of test group, addition is the 800g/ ton, the control group fed basal diet.Feed continuously after 30 days, volume of semen, sperm concentration, sperm motility and rate of teratosperm are measured in semen collection.
Result shows: with control group, compare, the breeding oxen volume of semen that adds physiological regulator for male animals of feeding changes not obvious, sperm concentration improves 19.05%, and (control group is 1.05 ± 0.12 hundred million/ml, test group is 1.25 ± 0.31 hundred million/ml), sperm motility improves 6.01% than control group, and (control group is 75.43%, test group is 79.96%), rate of teratosperm is than control group decline 30.88% (control group is 17.23%, and test group is 11.91%).This illustrates that physiological regulator for male animals of the present invention improves significantly to the Reproductive Performance of breeding oxen.
Test example 3
This test example is with the meat male earner chicken test of AA of feeding of the buck physiological regulation agent of the embodiment of the present invention 3 preparation
Choose the meat male earner chicken of 40 plumage AA, be divided at random two groups (test group: the basal diet of the physiological regulator for male animals that is added with the embodiment of the present invention 3 preparation of feeding, addition is the 300g/ ton; The control group fed basal diet), every group of 20 plumages.Feed after 30 days continuously, compare with control group, daily gain improves 12.1% (P<0.01), and in blood, testosterone concentration improves 11.9% (P<0.05).This illustrates that physiological regulator for male animals of the present invention can promote the Fast Growth of male earner chicken and strengthen Reproductive Performance.