CN109966426A - A kind of Chinese medicine immunomodulator of jujube Radix Astragali mixed fermentation and preparation method thereof - Google Patents
A kind of Chinese medicine immunomodulator of jujube Radix Astragali mixed fermentation and preparation method thereof Download PDFInfo
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Abstract
The present invention is to provide Chinese medicine immunomodulators of a kind of jujube Radix Astragali mixed fermentation and preparation method thereof, the additive is that the probiotics fermention liquid progress solid fermentation of the addition 3 ~ 5% in fermentation raw material forms, and 40~55 g of jujube, 10~15g of Radix Astragali, 15~20g of clover, 2~5g of dregs of beans, 4~10g of corn, 2~5g of digested tankage, 2~5g of insect protein are contained in every 100 g fermentation raw material.Contain probiotics two or more in lactobacillus plantarum, lactobacillus acidophilus, clostridium butyricum, saccharomycete, bacillus subtilis or lactic acid coccobacillus in the probiotics fermention liquid.The present invention has unique fermentation fragranced, it is full of nutrition, the synergistic effect of probiotics and Chinese medicine can be played, the Tiny ecosystem colony balance for safeguarding body gastrointestinal tract, can improve animal immunizing power, it can also be used to improve the appetite and appetite of mammal, improve nutrition of breast milk content, enhance dam immunity of organisms, reduce disease incidence, promotes the growth and development of cultivated animals body.
Description
Technical field
The invention patent belongs to the scope of additive, and in particular to a kind of Chinese medicine of the mixed fermentations such as jujube Radix Astragali is immune to be adjusted
Save agent and preparation method thereof.
Background technique
Chinese medicine immunomodulator refer to anti-infective, antiviral and antitumor, antiallergic action, in terms of have increase
Chinese patent drug (including some extracts) that is strong and adjusting immune function, be clinically mainly used for infectious diseases, it is certain from
The adjuvant treatment of body immunological disease and tumour.With the abuse of antibiotic and getting worse for livestock products medicament residue problem, food
Safety problem is increasingly subject to the concern of people.Chinese medicine becomes the coke that everybody studies with the advantage of its " safety, green, noresidue "
Point, Chinese medicine and probiotic etc. become the substitute for replacing antibiotic.Collect using prepared by some beneficial microbe fermented tcms
The Chinese medicine immunomodulator that " Chinese medicine, probiotic " is integrated, it will more effectively push the sound development of animal husbandry and fishery industry.
Microbial flora can generate a variety of enzymes such as cellulase, lignoenzyme, lipase during growth metabolism, simultaneously
With a variety of biotransformation capacities such as oxidation, esterification, methylation, hydroxylating, reducing.Contain protein, sugar, rouge in Chinese medicine
A variety of nutriments such as fat, amino acid, vitamin, microelement, organic acid, enzyme, alkaloid, polysaccharide, glucoside, tannin.
Microbial fermentation Chinese medicine is to combine Modern microbiological with traditional Chinese medicine research, by Chinese medicine in preferred beneficial bacteria of intestinal tract flora one
Kind or several, one plant or several probiotics be strain, under suitable environmental condition, biology is carried out to effective component of chinese medicine and is turned
Change, the macromolecular substances in Chinese medicine are transformed into the small-molecule substance that can be directly absorbed by animal body enteron aisle, make Chinese medicine at
Quickly to absorb, the newtype drug of quantitative curative effect.Microbial fermentation Chinese medicine can increase the utilization rate of effective component of chinese medicine, improve
The drug effect of Chinese medicine.The synergistic effect of probiotics and Chinese medicine can be played by fermenting, Chinese medicine can promote the proliferation of probiotics, prebiotic
Bacterium can also promote the absorption and utilization of Chinese medicine.Chinese medicine after microbial fermentation, can by the integrally-regulated of animal body and
Excite the active factor of all antibacterials of body, i.e. nospecific immunity antimicrobial factors, the comprehensive killing cause of disease in animal body
Bacterium, while not developing drug resistance.Using integrating the immune of " Chinese medicine, probiotic " prepared by beneficial microbe fermented tcm
Regulator improves in the growth and development for promoting cultivated animals body immunity of organisms, safeguards the micro-ecological bacterial of body gastrointestinal tract
Group's balance etc. is played a positive role.
It is recorded in the herbal medicine books Shennong's Herbal of China, jujube warm-natured, returns spleen stomach meridian sweet in flavor, there is tonifying middle-Jiao and Qi, blood-nourishing peace
Mind, the function of mitigating pharmacological property；And modern pharmacology then finds that jujube contains protein, fat, carbohydrate, organic acid, vitamin
A, vitamin C, lot of trace calcium, rich in the nutrition abundant such as more rich polyphenol, Flavonoid substances and amino acid.It is red
Jujube has the effects that the effect of invigorating spleen and reinforcing stomach, benefiting qi and nourishing blood, tranquilizing and allaying excitement, especially invigorating spleen and reinforcing stomach is obvious, while there are also enhance
Immunity of organisms, enhancing muscular strength, put on weight, the effect of benefiting qi and nourishing blood, are mainly used in field of food at present, in feed
There is very big development prospect in industry.
Radix Astragali also known as astragalus refer to the root of leguminous plant Radix Astragali, have invigorating qi for strengthening superficies, profit water detumescence, pus draining and toxin expelling, myogenic etc.
Effect.Radix Astragali, which contains, has more than 100 multiple compounds, including astragalus polyose (APS), flavones, saponin, a variety of amino acid and micro member
Element had not only had abundant nutrition but also had had pharmacodynamic feature.Astragalus polyose have always been considered as be astragalus root pod membrane main pharmacological property substance
Ingredient plays important pharmacological action in terms of reducing oxidative stress, promoting metabolism.Astragalus polyose, which can improve, exempts from antigen
The conversion ratio of epidemic disease responsibility and lymphocyte, and the phagocytic activity of macrophage can be enhanced；Early weaning to weanling pig
Stress, there is apparent immunological enhancement.The daily gain of weanling pig can be improved in addition astragalus polyose, reduces diarrhea rate,
Weanling pig is also improved to the digestibility of nutriment simultaneously.And astragalus polyose can be made largely to discharge by fermentation, it sends out
Ferment formulation of astragalus root plays the role of increasing main immunoglobulin and partial immunity cytokine content in weanling pig blood, thus
Enhance the disease resistance of weanling pig.Modern research shows that the raw Radix Astragali of salt has heart tonifying diuresis, antihypertensive effect, to dysentery, charcoal
Subcutaneous ulcer, corynebacterium diphtheriae, staphylococcus, streptococcus, Diplococcus pneumopniae have antibacterial efficacy, as feed addictive, have quickening machine
The effect of body metabolism, promotion animal growth and development.
Currently, extensive in every country and area using only a kind of fermented product of probiotics fermention jujube Radix Astragali etc.
Using, but lactobacillus plantarum, lactobacillus acidophilus, clostridium butyricum, saccharomycete, bacillus subtilis and lactic acid coccobacillus etc. is a variety of
Probiotic combination gets up to ferment the Chinese medicines such as jujube, Radix Astragali, is finally made the immunomodulator that " Chinese medicine, probiotics " is integrated, makes
Its product for playing probiotics and Chinese medicine synergistic effect is there is not yet reported in literature.
Summary of the invention
The purpose of the present invention is to provide a kind of Chinese medicine immunomodulators of the mixed fermentations such as jujube Radix Astragali, while providing it
Corresponding preparation method is another goal of the invention of the invention.
Based on above-mentioned purpose, the present invention is adopted the following technical scheme that: a kind of Chinese medicine of the mixed fermentations such as jujube Radix Astragali is immune
Regulator is that the probiotics fermention liquid progress solid fermentation of the addition 3~5% in fermentation raw material forms, every 100 g fermentation raw material
In contain 40~55 g of jujube, 10~15g of Radix Astragali, 15~20g of clover, 2~5g of dregs of beans, 4~10g of corn, 2~5g of digested tankage, elder brother
2~5g of worm albumen.
The probiotics fermention liquid is cultured probiotic's culture liquid, and probiotics includes lactobacillus plantarum, acidophilus cream bar
Two or more mixing in bacterium, clostridium butyricum, saccharomycete, bacillus subtilis or lactic acid coccobacillus.
A kind of preparation method of the Chinese medicine immunomodulators of mixed fermentations such as jujube Radix Astragali, comprising the following steps:
(1) it crushes and feeds intake: the raw materials such as jujube, Radix Astragali, clover, corn, dregs of beans are crushed, cross 40-80 mesh, regulation is quasi- according to the proportion
Each raw material needed for really weighing is put into batch can, and dry-mixed 20-30min is stirred evenly.
(2) add bacterium solution: the probiotics fermention liquid of thrown raw material weight 3 ~ 5% being added in above-mentioned batch can, stirs evenly, to
With.
(3) add water: (water temperature is 35~50 for the warm water of addition raw material weight 30% in the fermented material of step (2) preparation
DEG C), add the water time-division to be repeatedly added on a small quantity, stirs evenly.
(4) it fills round: the raw material stirred evenly in step (3) is fitted into round, be compacted, sealing makes to send out
Ferment container is completely enclosed, airtight, manufactures anaerobic environment, carries out anaerobic fermentation；
(5) it ferments: the round installed is pushed into fermenting cellar, fermentation room temperature is 35-40 DEG C；Continuing fermentation 5-7d；
(6) dry: fermentation time terminates, and tunning rapid draing in round (is dried or dried), drying time is not
Preferably more than 2d to get the Chinese medicine immunomodulator of the mixed fermentations such as the jujube Radix Astragali.
When the Chinese medicine immunomodulator of the mixed fermentations such as jujube Radix Astragali of the present invention is adjusted for animal immune, pressed in feed
It is added according to the amount of the 1-10% of feed relative.
Heretofore described digested tankage mainly uses fresh Animal Skin, viscera tissue etc., adds by special technique
The nutritional supplement that work is eaten at animal feed additive or the mankind.Digested tankage protein content is high, also containing a large amount of calcium, phosphorus and
Manganese, full of nutrition, conducive to being absorbed and utilized for raising animal, stimulating growth and breeding to some extent is other nutriment institutes
It is irreplaceable.
Heretofore described clover is the common name of clover category (Medicago) plant, is commonly called as " clover ", is China's plantation
The maximum Perennial legume forages of area.Alfalfa regeneration power is strong, can gather within 1 year repeatedly, and the protein content of tree alfalfa is very high,
17 kinds of amino acid contents more balance, and various microelements and the vitamin such as calcium, phosphorus, zinc, selenium needed rich in a variety of domestic animals contain
There are more edible fibers, tree alfalfa saponin contained in tree alfalfa can promote cholesterol to drain, and have prevention and slow down light
The effect of dynamic fat hardening.Polysaccharide component in tree alfalfa, is alternatively arranged as immunologic adjuvant, there is immune-enhancing effect.In addition to this, lucerne
Mu tree also has a variety of dietotherapies and medical value, for example, can promote to become pregnant with it is pregnant, act on having stimulating milk secretion；There are also diuresis and gently
The effect rushed down；To urinary system and alimentary canal, respiratory disease, there is therapeutic effect；There are also trichogenous effects.Cause
This, clover has highly important value and status in animal husbandry development.
There is stimulating gastrointestinal to wriggle, accelerate excrement excretion for plant cellulose rich in heretofore described corn
Characteristic can prevent treating constipation, enteritis, intestinal cancer etc..Inhibition blood glucose liter after meal is played in the absorption that can fetter and hinder excessive glucose
High effect；Cellulose can also inhibit fat absorption, reduce blood lipid level, prevention and improvement coronary heart disease, obesity, gall stone
Generation.The selenium and magnesium contained in corn has cancer-resisting effect, and the decomposition of selenium energy acceleration bodies endoperoxides object obtains malignant tumour
Less than molecular oxygen supply and be suppressed.On the one hand magnesium can also inhibit the development of cancer cell, on the other hand can promote useless in vivo
Object excretes, this is also significant to giving protection against cancer.
A kind of byproduct obtained after heretofore described dregs of beans soybean extracting bean oil.Also known as " Soybean Meal ", be Cottonseed Meal,
Yield is maximum in 12 kinds of animals and plants oil meal feed products such as peanut meal, rapeseed dregs, the most wide one kind of purposes.As a kind of high protein
Matter, dregs of beans are the primary raw materials for making livestock and poultry feed, can be also used for production cake food, healthy food and makeup
Product and antibiotic material.
Heretofore described insect protein be using insect as raw material, from each growth phase of insect, as ovum, larva, at
The protein of the extractions such as worm, pupa, moth.Insect is the maximum monoid in the animal kingdom, and biomass is more than other all animals (including people
Class) 10 times or more of biomass summation.A large amount of Nutritional studies prove, protein are rich in insect bodies, fiber is few, micro member
Element is abundant, is easy to absorb, is the largest animal protein resource；Simultaneously rich in unsaturated fatty acid, polysaccharide, antibacterial peptide etc. effectively at
Point, it is important renewable organic resource.The antibacterial peptide components that insect protein has have activation immunity, effectively prevent stomach
The effect of intestinal inflammatory and various infectious diseases has great as high-quality health-care feed and biological medicine raw material
Potentiality.
Heretofore described lactobacillus plantarum lactobacillus plantarum is one kind of lactic acid bacteria, belongs to homofermentative lactic bacteria, is
Anaerobic bacteria.Lactobacillus plantarum has many health-care effects, than having inhibiting effect to pathogenic bacteria if any immunoregulation effect,
Serum cholesterol content and prevention cardiovascular disease can be reduced, intestinal flora balance is maintained, promotes absorption of nutrient ingredients and suppression
The effects of formation of tumour cell processed.
Heretofore described lactobacillus acidophilus is stomach and enteral main probiotics, belongs to gram-positive cocci, obligate
Anaerobism can adjust intestinal flora balance, inhibit the proliferation of enteron aisle undesirable microorganism, can secrete antibiotin substance (acidophilus
Lactobacillin, bacillus acidophilus's element, lactein etc.) antagonism is generated to pathogenic entero becteria.
Heretofore described clostridium butyricum belongs to fusobacterium, is Gram-positive anaerobic bacillus(cillus anaerobicus).Clostridium butyricum has whole
Intestines, improve the effects of immunity at anticancer, can inhibit the growth and breeding of harmful bacteria and corrupt group in enteron aisle, and Miyarisan is most significant
Biological nature be that it and can promote its development proliferation with the intestinal beneficial bacteriums symbiosis such as lactobacillus acidophilus.
Heretofore described saccharomycete is the unicellular of a kind of shape round in cell, ellipse or cylindrical vegetative propagation
The important fungi of biology.There is very high nutritive value, especially containing compared with polyprotein, many B family vitamins, nucleic acid and mineral
Matter, while some healthcare function active materials can be also generated, body's immunity can be enhanced.
Heretofore described bacillus subtilis is one kind of bacillus, during bacillus subtilis thalli growth
Subtilin, polymyxins, nystatin, the gramicidins isoreactivity substance of generation, these active materials are to pathogenic bacteria or interior
The conditioned pathogen of source sexuality dye has apparent inhibiting effect.The free oxygen in enteron aisle can be consumed rapidly, cause enteron aisle hypoxemia,
Promote to grow beneficial to anaerobic bacteria, other pathogenic bacteria is inhibited to grow indirectly；The growth and development of animal (human body) immune organ is stimulated, is swashed
T, bone-marrow-derived lymphocyte living, improve immunoglobulin and antibody level, enhance cellular immunity and humoral immune function, improve group and exempt from
Epidemic disease power.
Heretofore described lactic acid coccobacillus is one of flora dominant in animals and humans enteron aisle, it can prevent disease
Invasion and field planting of the opportunistic pathogen to enteron aisle inhibit pathogen, anti-infective, maintain the microecological balance of enteron aisle, prevention and inhibition tumour
Generation, enhance immunity of organisms, promote digestion, synthesizing amino acid and vitamin reduce cholesterol, inhibits endotoxic life
It produces, delays senescence and anti-radiation etc..
The microorganism of used fermentation is all the strain beneficial to animal body by screening in the present invention, such as plant cream bar
Bacterium, bacillus, saccharomycete etc., themselves just has healthcare function, such as promotes the growth and development of body, improves immunity of organism
Power generates the substances such as digestive ferment, acids in animal intestinal tract, safeguards Tiny ecosystem colony balance of body gastrointestinal tract etc..In natural
In medicine containing protein, sugar, fat, amino acid, vitamin, microelement, organic acid, enzyme, alkaloid, polysaccharide, glucoside,
A variety of nutriments such as tannin and growth promoting activity substance.Probiotics and Chinese medicine are complementary in disease prevention growth-promoting long side.
Chinese medicine after fermentation because curative effect enhances, therefore can reduce dosage, to reduce the cost of bulk pharmaceutical chemicals.Chinese medicine hair is supplemented in feed
Ferment object can be effectively improved the feed nutrient utilization rate of grower pigs；Promote commodity pig feed intake, improves daily gain, reduce feedstuff-meat ratio
Effect, and it can be effectively improved the Slaughter of growing and fattening pigs, intramuscular fat content is dramatically increased, meat quality is improved.Largely grind
Study carefully and show that Chinese medicine and probiotic are used in combination effect and are used alone better than Chinese medicine, the special family rate of feed that group is used in combination improves, abdomen
Rate reduction is rushed down, serum globulins content improves.
Specifically, the invention has the benefit that
(1) present invention is got up to ferment the Chinese medicines such as jujube, Radix Astragali and clover using a variety of probiotic combinations for the first time, be finally made " in
The Chinese medicine immunomodulator that medicine, probiotics " is integrated, makes it play probiotics and Chinese medicine synergistic effect, both has maintenance body
The function of gastrointestinal bacterial flora balance, while can be good at improving immunity of organisms.
(2) present invention releases that the effective component in the Chinese medicines such as jujube, Radix Astragali, clover sufficiently by probiotics fermention means
It puts, the macromolecular substances in Chinese medicine is transformed into the small-molecule substance that can be directly absorbed by animal body enteron aisle, increase
The utilization rate of pharmaceutically active ingredient improves the drug effect of Chinese medicine.
(3) it is a variety of to generate cellulase, lignoenzyme, amylase, protease etc. in the metabolic process for microbial flora
Enzyme can promote the digestion and absorption of animal, while have a large amount of acidic materials in the metabolite of probiotics, can inhibit harmful bacteria
Growth, improve animal disease resistant ability, thus reduce make with or without antibiotic medicine, additive of the invention promote cultivate
It will play a significant role in the sound development of industry.
(4) immunomodulator of the present invention is fully natural green product, and production process energy conservation and environmental protection is residual without drug after use
It stays, can accomplish no three wastes environmental requirement.Meanwhile the main raw material sources of the present invention are extensive, are easy to get, cost of manufacture is lower, tool
There are good economic benefit and social benefit.
(5) present invention has unique fermentation fragranced, full of nutrition, and can play probiotics and Chinese medicine cooperates with work
With, safeguard the Tiny ecosystem colony balance of body gastrointestinal tract, can be not only used for improve mammal appetite and appetite, improve milk battalion
Content is supported, dam immunity of organisms is enhanced, reduces disease incidence, promotes the growth and development of cultivated animals body.
(6) preparation method rational technology of the present invention, production is simple, strong operability, is suitable for large-scale production.
Specific embodiment
Following example is only to further illustrate the present invention, and the invention is not limited in any way.
Embodiment 1
A kind of Chinese medicine immunomodulator of the mixed fermentations such as jujube Radix Astragali is the probiotics fermention liquid that 3% is added in fermentation raw material
Carry out solid fermentation form, in every 100 g fermentation raw material containing jujube 40g, Radix Astragali 15g, clover 20g, dregs of beans 5g, corn 10g,
Digested tankage 5g, insect protein 5g.
The probiotics fermention liquid is cultured probiotic's culture liquid, and probiotics includes lactobacillus plantarum, acidophilus cream bar
Bacterium.
Preparation method, comprising the following steps:
(1) it crushes and feeds intake: the raw materials such as jujube, Radix Astragali, clover, corn, dregs of beans are crushed, cross 40 mesh, regulation is accurate according to the proportion
Each raw material needed for weighing is put into batch can, and dry-mixed 20min is stirred evenly.
(2) add bacterium solution: the probiotics fermention liquid of thrown raw material weight 3% being added in above-mentioned batch can, stirs evenly, for use.
(3) add water: the warm water (water temperature is at 35 DEG C) of raw material weight 30% being added in the fermented material of step (2) preparation, adds
The water time-division is repeatedly added on a small quantity, stirs evenly.
(4) it fills round: the raw material stirred evenly in step (3) is fitted into round, be compacted, sealing makes to send out
Ferment container is completely enclosed, airtight, manufactures anaerobic environment, carries out anaerobic fermentation.
(5) it ferments: the round installed is pushed into fermenting cellar, fermentation room temperature is 35 DEG C；Continuing fermentation 5d.
(6) dry: fermentation time terminates, and tunning rapid draing in round (is dried or dried), when dry
Between no more than 2d.Up to the Chinese medicine immunomodulator of the mixed fermentations such as the jujube Radix Astragali.
Embodiment 2
A kind of Chinese medicine immunomodulator of the mixed fermentations such as jujube Radix Astragali is the probiotics fermention liquid that 5% is added in fermentation raw material
Carry out solid fermentation form, in every 100 g fermentation raw material containing 55 g of jujube, Radix Astragali 15g, clover 20g, dregs of beans 2g, corn 4g,
Digested tankage 2g, insect protein 2g.
The probiotics fermention liquid is cultured probiotic's culture liquid, and probiotics includes clostridium butyricum, saccharomycete, lactic acid
Coccobacillus.
Preparation method, comprising the following steps:
(1) it crushes and feeds intake: the raw materials such as jujube, Radix Astragali, clover, corn, dregs of beans are crushed, cross 80 mesh, regulation is accurate according to the proportion
Each raw material needed for weighing is put into batch can, and dry-mixed 10min is stirred evenly.
(2) add bacterium solution: the probiotics fermention liquid of thrown raw material weight 5% being added in above-mentioned batch can, stirs evenly, for use.
(3) add water: the warm water (water temperature is at 50 DEG C) of raw material weight 30% being added in the fermented material of step (2) preparation, adds
The water time-division is repeatedly added on a small quantity, stirs evenly.
(4) it fills round: the raw material stirred evenly in step (3) is fitted into round, be compacted, sealing makes to send out
Ferment container is completely enclosed, airtight, manufactures anaerobic environment, carries out anaerobic fermentation.
(5) it ferments: the round installed is pushed into fermenting cellar, fermentation room temperature is 40 DEG C；Continuing fermentation 7d.
(6) dry: fermentation time terminates, and tunning rapid draing in round (is dried or dried), when dry
Between no more than 2d.Up to the Chinese medicine immunomodulator of the mixed fermentations such as the jujube Radix Astragali.
Embodiment 3
A kind of Chinese medicine immunomodulator of the mixed fermentations such as jujube Radix Astragali is the probiotics fermention liquid that 4% is added in fermentation raw material
Carry out solid fermentation form, in every 100 g fermentation raw material containing 55 g of jujube, Radix Astragali 12g, clover 18g, dregs of beans 3g, corn 6g,
Digested tankage 3g, insect protein 3g.
The probiotics fermention liquid be cultured probiotic's culture liquid, probiotics include lactobacillus acidophilus, clostridium butyricum,
Saccharomycete, lactic acid coccobacillus.
Preparation method, comprising the following steps:
(1) it crushes and feeds intake: the raw materials such as jujube, Radix Astragali, clover, corn, dregs of beans are crushed, cross 60 mesh, regulation is accurate according to the proportion
Each raw material needed for weighing is put into batch can, and dry-mixed 10min is stirred evenly.
(2) add bacterium solution: the probiotics fermention liquid of thrown raw material weight 4% being added in above-mentioned batch can, stirs evenly, for use.
(3) add water: the warm water (water temperature is at 40 DEG C) of raw material weight 30% being added in the fermented material of step (2) preparation, adds
The water time-division is repeatedly added on a small quantity, stirs evenly.
(4) it fills round: the raw material stirred evenly in step (3) is fitted into round, be compacted, sealing makes to send out
Ferment container is completely enclosed, airtight, manufactures anaerobic environment, carries out anaerobic fermentation.
(5) it ferments: the round installed is pushed into fermenting cellar, fermentation room temperature is 37 DEG C；Continuing fermentation 6d.
(6) dry: fermentation time terminates, and tunning rapid draing in round (is dried or dried), when dry
Between no more than 2d.Up to the Chinese medicine immunomodulator of the mixed fermentations such as the jujube Radix Astragali.
The experiment of 4 application effect of embodiment: by measuring the indexs such as mice spleen lymphocytes proliferation and Mouse Liver spleen index,
Study influence of the Chinese medicine immunomodulator of the mixed fermentations such as jujube Radix Astragali prepared by the present invention to mouse body's immunity.
1, splenic lymphocytes culture experiment
Use the Chinese medicine immunomodulator of the mixed fermentations such as the jujube Radix Astragali of 3 embodiments preparation of the invention as test specimen, it is raw
Salt water is managed as blank sample, the mouse of adaptive feeding after a week is dissected 10, the spleen of mouse is extracted, carries out spleen lymph
Cell culture test.Measure shadow of the Chinese medicine immunomodulator to mice spleen lymphocytes proliferation of the mixed fermentations such as jujube Radix Astragali
It rings.
Inducing action of 3 immunomodulator of table to mice spleen lymphocytes proliferation
| Group | Immunomodulator preparation method | Cell number (a/mL) after culture 48h |
| Experimental group 1 | The preparation gained of embodiment 1 | 3.8×108 |
| Experimental group 2 | The preparation gained of embodiment 2 | 3.5×108 |
| Experimental group 3 | The preparation gained of embodiment 3 | 3.4×108 |
| Compare blank group | Do not add immunomodulator | 3.0×106 |
Lymphocyte plays important function as immunocyte in terms of animal immunizing power.Through micro- sem observation, culture 48
After hour, experimental group can be clearly visible a large amount of cell colony, from table 3 it can be seen that compared with the control group, all experimental groups
Cell number is significantly more than control group, illustrates the Chinese medicine immunomodulator of the mixed fermentations such as jujube Radix Astragali prepared by the present invention to small
Mouse spleen lymphocyte proliferation has stronger induction facilitation, thus increase the immunocyte of mouse, body immunity
It improves.
2, mouse liver index, index and spleen index measurement
Experiment mice is bought back, adaptive feeding 7d, and mouse is randomly divided into experimental group 1, experimental group 2, experimental group 3 and blank pair
According to group (every group 20), the jujube for adding preparation of the embodiment of the present invention in feed respectively to each experimental group and control animals is yellow
The Chinese medicine immunomodulator of the mixed fermentations such as stilbene, dosage are daily every 0.5 g, are continuously come into operation 30 days；Blank control group animal
Normal diet is then only fed, immunomodulator is not added.Feeding is put to death after mouse is weighed after 30 days, takes liver and spleen respectively
It is dirty, it weighs after being blotted with filter paper, liver index, index and spleen index is calculated according to the following formula respectively.
Liver index=liver mass (mg)/weight (g)
Index and spleen index=spleen weight (mg)/weight (g)
Liver index, index and spleen index measurement result are as shown in following table 4.
Influence of the immunomodulator of the present invention of table 4 to mouse liver index, index and spleen index
| Group (n=20) | Immunomodulator preparation method | Liver index (mg/g) | Index and spleen index (mg/g) |
| Experimental group 1 | The preparation gained of embodiment 1 | (52.05 ± 5.92): 1* | (3.23 ± 0.11): 1* |
| Experimental group 2 | The preparation gained of embodiment 2 | (48.69 ± 6.01): 1* | (3.18 ± 0.23): 1* |
| Experimental group 3 | The preparation gained of embodiment 3 | (50.12 ± 6.03): 1* | (3.54 ± 0.35): 1* |
| Compare blank group | Do not add immunomodulator | (38.22 ± 6.12): 1 | (2.61 ± 0.32): 1 |
Note: " * " indicates significant difference (P < 0.05) compared with the control group.
Spleen is peripheral immune organ, and liver is maximum digestive organs, while also assisting in the immune of body, therefore liver and spleen index
It is the most basic and most conventional index for reflecting body's immunity, body's immunity to a certain extent can be with immune organ
Index rises and increases.Experimental mice liver index, index and spleen index are bigger than control group as can be seen from Table 4, data point
Compared with the control group, there were significant differences (P < 0.05), shows system of the present invention for analysis display experimental mice liver index, index and spleen index
The Chinese medicine immunomodulator of the mixed fermentations such as standby jujube Radix Astragali grows to have to liver, the spleen of mouse to be promoted to make well
With so as to preferably improve the body immunity of mouse, therefore capable of being clinically used in Immune-enhancing effect.
Claims (5)
1. a kind of Chinese medicine immunomodulator of jujube Radix Astragali mixed fermentation, which is characterized in that be that 3 ~ 5% are added in fermentation raw material
Probiotics fermention liquid carry out solid fermentation form, in every 100 g fermentation raw material containing 40~55 g of jujube, 10~15g of Radix Astragali,
15~20g of clover, 2~5g of dregs of beans, 4~10g of corn, 2~5g of digested tankage, 2~5g of insect protein.
2. the Chinese medicine immunomodulator of jujube Radix Astragali mixed fermentation as described in claim 1, which is characterized in that the probiotics
Fermentation liquid be cultured probiotic's culture liquid, probiotics include lactobacillus plantarum, lactobacillus acidophilus, clostridium butyricum, saccharomycete,
Two or more mixing in bacillus subtilis or lactic acid coccobacillus.
3. a kind of preparation method of the Chinese medicine immunomodulator of jujube Radix Astragali mixed fermentation, which comprises the following steps:
(1) it crushes and feeds intake: jujube, Radix Astragali, clover, corn, dregs of beans raw material being crushed, 40-80 mesh is crossed, regulation is accurate according to the proportion
Each raw material needed for weighing is put into batch can, and dry-mixed 20-30min is stirred evenly；
(2) add bacterium solution: the probiotics fermention liquid of thrown raw material weight 3 ~ 5% being added in above-mentioned batch can, stirs evenly, for use；
(3) add water: the warm water of raw material weight 30% being added in the fermented material of step (2) preparation, the water time-division is added repeatedly to add on a small quantity
Enter, stirs evenly；
(4) it fills round: the raw material stirred evenly in step (3) is fitted into round, be compacted, sealing holds fermentation
Device is completely enclosed, airtight, manufactures anaerobic environment, carries out anaerobic fermentation；
(5) it ferments: the round installed is pushed into fermenting cellar, fermentation room temperature is 35-40 DEG C；Continuing fermentation 5-7d；
(6) dry: fermentation time terminates, by tunning rapid draing in round, drying time no more than 2d to get
The Chinese medicine immunomodulator of the jujube Radix Astragali mixed fermentation.
4. preparation method according to claim 3, which is characterized in that the water temperature of warm water is at 35~50 DEG C in step (3).
5. preparation method according to claim 3, which is characterized in that rapid draing mode in step (6) be drying or
It dries.
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| CN110812408A (en) * | 2019-12-03 | 2020-02-21 | 六安恒佳生物科技有限公司 | Liquid fermented traditional Chinese medicine preparation for preventing and controlling piglet diarrhea and preparation method thereof |
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| CN116138462A (en) * | 2023-03-29 | 2023-05-23 | 杭州知零生物科技有限公司 | A metagen composition for enhancing resistance and its preparation method |
| CN116439315A (en) * | 2023-04-21 | 2023-07-18 | 南京中医药大学 | Clostridium butyricum fermentation product of astragalus membranaceus stem and leaf as well as preparation method and application thereof |
| CN116439315B (en) * | 2023-04-21 | 2024-04-26 | 南京中医药大学 | Clostridium butyricum fermentation product of astragalus membranaceus stem and leaf as well as preparation method and application thereof |
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Application publication date: 20190705 |