CN109601699A - A kind of functional microbial fermented feed and production method and application - Google Patents
A kind of functional microbial fermented feed and production method and application Download PDFInfo
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- CN109601699A CN109601699A CN201811627977.0A CN201811627977A CN109601699A CN 109601699 A CN109601699 A CN 109601699A CN 201811627977 A CN201811627977 A CN 201811627977A CN 109601699 A CN109601699 A CN 109601699A
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- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/231—Lactis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of production methods of functional microbial fermented feed.Using in dregs of beans, fulvic acid powder, Dried Corn Steep Liquor Powder, wheat bran skin, bran powder and Chinese medicine CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, radix scutellariae powder, astragalus membranaceus powder, Radix Isatidis powder as fermentation raw material, using bacillus subtilis, bacillus licheniformis, Lactococcus lactis, beer yeast slurry as beneficial microbe colony.Bacillus subtilis, bacillus licheniformis, the seed liquor of Lactococcus lactis and beer yeast slurry are mixed in proportion with fermentation raw material, by aerobic fermentation, anaerobic fermentation, it is passed through filtrated air fermentation and anaerobic fermentation four-stage, obtains a kind of functional microbial fermented feed.Product of the present invention is full of nutrition, has good attractant, disease-resistant, protection effect is good, promotes cultivated animals growth result obvious.At the same time it can also improve breeding environment, purifies water, be a kind of green, safe and efficient functional microbial fermented feed.
Description
Technical field
The present invention relates to Animal nutrition and technical field of microbial fermentation, in particular to a kind of functional microbial fermentation is raised
The production method of material.
Background technique
China's feedstuff industry is in the development course in more than 40 years of passing by, and grow out of nothing and expand from small to large for feed industry, from weak
To strong.By the end of 2017, national large database concept statistical result 22161.2 ten thousand tons of Chinese commodity feed total output in 2017,
19618.6 ten thousand tons of middle mixed feed yield, 1853.7 ten thousand tons of concentrated feed yield, additive premix yield 688.9
Ten thousand tons.
Possess Feed Manufacturing licensing enterprise 7908, has outside 50 500,000 tons or more enterprise groups, most of enterprise year
Yield is less than 10,000 tons.Above-mentioned data are China's second generation formula forage authority's master data.
How a large amount of excessive use feed protein and too early excessive two big outstanding problems using antibiotic are solved?
An only approach produces third generation feed, Formula type fermentative feedstuff of microbe.
It is well known that best high-quality protein source is fish meal and dregs of beans, quality is best for peru fish meal in fish meal, belongs to
Animal protein, protein content is high, and rationally, lysine content is high, and freshness is good, in the shrimp of high quality for amino acid composition
In feed processing, peru fish meal is added, the attractant of feed and various economic indicator performance highlight.Dregs of beans is measured most in protein sources
Greatly, kind the most convenient is processed, is that protein content is low with fish meal comparative disadvantage, lysine content is low, and has antigen anti-
It answers.The source in high-quality protein source is solved with this, substitutes fish meal.By formula forages such as dregs of beans, using outstanding in beneficial microbe
Flora carries out liquid solid fermentation under conditions of artificial controllable, by the breaks down proteins in formula forage at small peptide and amino
Cellulose and starch are resolved into glucide by acid, can also generate a large amount of amino acid, organic acid, lipid by fermentation
Matter, immune factor and promote growth factor, there are also a large amount of beneficial microbes to exist in fermentation material, constantly generates new nutrition
The factor and antibacterial peptide etc..Animal autoimmunity can be improved in these trophic factors, promotes to digest and assimilate, can be strong with salubrity
Body.
How the protein in dregs of beans to be improved, lysine is improved, antigen-reactive is removed, improves efficiency of feed utilization,
Always be the researcher and enterpriser of animal nutrition and feed nutrient formulation research and development forever continue project.
At the beginning of 21 century, small peptide is produced using bean pulp fermentation, in beasts, birds and aquatic products animal husbandry, TaiWan, China is derived from, newly adds
Slope and Southeast Asia.Then, from South Korea, Japan from the incoming China in the north, China's research and development, production and application are opened from Chinese southern areas
Begin.
National large database concept statistical result, China's feed total output in 2017 are 2.2 hundred million tons, feed addictive total output
1034.7 ten thousand tons, the feed industry gross output value is 839,300,000,000 yuan.Wherein, it is related to 38,460,000 tons of (ammonia of product of microbial fermentation production
234.8 ten thousand tons of base acid, accounting 23%.7.4 ten thousand tons of vitamin 12, accounting 12%.10.7 ten thousand tons of microorganism formulation, accounting 1%.Enzyme
10.7 ten thousand tons of preparation, accounting 1%), accounting 37.0%.If according to 10,000 yuan of 1 ton of calculating, 384.6 hundred million yuan.
It is exactly that annual production capacity will reach 38,000,000 tons, the output value 380 hundred million if fermented feed replacing whole function.Half
Substitute 20,000,000 tons, 200 hundred million yuan.These data are the yield prospects of development.
At abroad, protide feed addictive, amino acids feed addictive, microorganisms feed additive, main raw
Produce country and company be the ADM company in the U.S., method national music this good fortune, hundred good fortune of Switzerland, Norway it is refined come, Japanese aginomoto, Denmark's promise and
The biotech companies such as Nuo De.
China is added using micro-organisms protide feed addictive, amino acids feed addictive, microorganisms feed
Add agent, peptide feed addictive occupies global third position, and main manufacturer is Beijing Da Bei Nong, Guangdong Asia fine jade, Hubei Angel, Shandong
The health ground biotech companies such as grace, Shandong root.
Its main application result and function are as follows:
The benefit of fermenting organism feed is as follows:
1 improves efficiency of feed utilization, reduces cost
Biological feedstuff fermentation is by biochemical reaction, effectively feedstuff decomposition and inversion at glucose and amino
Acid, various effective ingredients are rapidly absorbed and used, and the utilization rate of feed can be improved.Ferment the fatty acid generated, organic acid
Increase energy in daily ration, weight gain and feed conversion rate can be improved.Ferment a variety of bacterium proteins and protein feed generated, shows
It writes and increases feed nutrient, reduce feed cost.
2 improve the palatability of feed
Feed after fermentation, generates natural sour taste, can stimulate the appetite of animal, promotes the secretion of digestive juice, improves
The activity of digestive ferment, accelerates the decomposition of feed nutrient, to promote the absorption of nutriment, so that the feeding animal feed intake of institute
It is improved comprehensively with grazing speed.
3 improve environment
Exist due to improving protein utilization rate and beneficial microorganism, by oxidation, ammonification, nitrification, denitrification, fastly
Toxic and harmful gas (such as ammonia, hydrogen sulfide that prompt drop solution remaining bait, the excreta of cultivated animals and animals and plants remnant generate
Deng), breeding environment can be improved, establish the ecosystem of a benign cultivated animals, microorganism and environment, be to resist cause of disease
One of the most effective mode of sexuality dye.
4 fermentation detoxifications, feed are safer
After feed link everfermentation, the lactic acid bacteria of generation can inhibit the growth of mould and produce poison, and Lactococcus lactis, which can inhibit, to be posted
The metabolite of the spore germination of raw aspergillus, bacillus subtilis and bacillus licheniformis can reduce containing for forage poisoning element
Amount.Fermented feed is lower than the harmful substance contents of non-fermented feed, for more being anticipated by the food-safety problem of growing interest
Justice.
The mechanism of action of fermentative feedstuff of microbe
1 supplement beneficial bacterium, adjusts the microecological balance of animal
Animal health will constitute the balance of microecosystem in animal and bird intestines, and animal supplement microbiological feed has very heavy
The meaning wanted.Animal continuously takes active bacteria formulation and is just able to maintain since many microorganisms can only survive a period of time in enteron aisle
Field planting of the microorganism in enteron aisle.
The effect of 2 chemical barriers
The organic acid (lactic acid, propionic acid, acetic acid) that beneficial microbe such as lactic acid bacteria class generate reduces pH value in alimentary canal, suppression
The growth of other pathogenic microorganisms is made, when environmental pH is 4, Escherichia coli and salmonella cannot be proliferated, and can promote minerals
The absorption and utilization of element calcium, phosphorus, iron and vitamin D.
3 generate beneficial metabolites, inhibit and kill harmful bacteria
Beneficial microbe can produce peptide bacteriocin, enzyme material, can inhibit the growth of harmful bacteria or prevent harmful bacterium
Adherency and intrusion of the element in epithelial cell.The Lactococcus lactis peptide that lactic acid bacteria and streptococcus generate can inhibit salmonella and large intestine
The growth of bacillus；Lactobacillus acidophilus can produce the growth that a small amount of catalase inhibits pathogen；Bacillus acidi lactici can prevent
Bacteriotoxin.
4 have trophism, promote growth of animal
Beneficial bacterium in fermentative feedstuff of microbe itself is a kind of high protein substance, and bacillus can secrete a variety of digestion
The digestion and absorption of enzyme (protease, amylase, lipase etc.) promotion nutriment；Saccharomycete can produce amino acid, a variety of
Vitamin (K, C, B1, B2, pantothenic acid, niacin, biotin, inositol and folic acid etc.) is utilized for animal body, and saccharomycete can also promote
The generation of phytase improves the utilization rate to phosphorus.
5 improve body's immunity
Beneficial microbe in fermented feed can produce nonspecific immunity regulatory factor, enhances B cell in animal body and produces
The ability of raw antibody, improves livestock and poultry antibody level, enhances immune function, kills the intracorporal pathogenic bacteria of intrusion machine in time, thus anti-
The only generation of disease.
In the research and development and production of previous fermentative feedstuff of microbe, people are mainly focused in the fermentation of protein, just
It is that the fermentation of the protides such as fermented bean dregs cotton dregs, rapeseed dregs is primary come a possibility that substituting the animal protein such as fish meal, there are also be exactly
Fermented cellulosic class, wheat bran skin, maize peel etc. degrade to its cellulose, generation carbohydrate and organic acid substance, and above-mentioned two
More description, test in detail has been made in research, achieves a large amount of science data and test result, such report compared with
It is more.But in the research and application of fermentative feedstuff of microbe, prominent based on one or two kinds of functionality, adding, other are several
Kind substance, with CORTEX PHELLODENDRI CHINENSE powder, the RHIZOMA COPTIDIS from Sichuan of China powder, Huang Qin in dregs of beans, fulvic acid powder, Dried Corn Steep Liquor Powder, wheat bran skin and Chinese medicine
Powder, Radix Astragali, Radix Isatidis are fermentation raw material, are with bacillus subtilis, bacillus licheniformis, Lactococcus lactis and beer yeast slurry
Fermentative microflora, wherein fulvic acid powder, CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, radix scutellariae powder, astragalus membranaceus powder have antiinflammation, bacillus subtilis
Bacterium, bacillus licheniformis, Lactococcus lactis, during the fermentation, can be metabolized out antibacterial peptide material, this type microbial fermentation
Feed plays the effect of 1+2 >=3.Such Formula type, functional microbial fermented feed are not reported simultaneously.
In recent years, with the fast development of aquaculture industry and feed industry, fermentative feedstuff of microbe and functional microbial
Fermented feed research constantly gos deep into application and extension, achieves very gratifying result of study.
Fulvic acid: powdery, dark-brown, sour, odorless, soluble easily in water, fulvic acid content >=95%, 10% aqueous solution
PH3.0.It can extract from mud coal, lignite, structure is aromatic hydroxy-carboxylic substance；Biochemical fulvic acid is from microbial fermentation
It is extracted in plant waste afterwards, composition is more complex, and other than aromatic hydroxy-carboxylic, there are also a certain number of water-soluble carbon water
Compound, amino acid, protein, saccharic acid substance.Element composition: C 54.82%H 2.29%O 41.14%N 0.66%S
1.09%；Function mass contg: 8.76 milliequivalent of total acidic group/gram (6.12 milliequivalent of hydroxyl/gram, 2.64 milligrams of phenolic hydroxyl group are worked as
Amount/gram), it is used as feed addictive, live pig appetite can be made to increase；Laying rate of chicken is set to improve 15.5%, the hatching rate of chicken improves
21.8%, chick rate improves 15-20%, prevents the disease of domestic animals and checken pest.
CORTEX PHELLODENDRI CHINENSE: for the bark of rutaceae wampee, main product is in Sichuan.It is bitter in taste, cold, return kidney, bladder meridian, has
The effect of heat-clearing and damp-drying drug, purging intense heat, except steaming, detoxification sore treatment, for treating the diseases such as damp-heat dysentery, jaundice, leukorrhagia, heat gonorrhea, hectic fever due to yin labor heat
Disease.
RHIZOMA COPTIDIS from Sichuan of China: coptis bitter often claims " three is yellow " with radix scutellariae, Cortex Phellodendri.Have the function of eliminating dampness heat-clearing, purging fire for removing toxin.River
The coptis, radix scutellariae and radix aucklandiae, compatible baked ginger charcoal are the effective product for controlling chronic colitis.
Radix scutellariae: there is wider antimicrobial spectrum, infected by influenza, dermatophyte also have effect, be clinically used for treating viral
Eye disease and the infection of the upper respiratory tract have good therapeutic effect.
Bacillus subtilis (Bacillus subtilis): being gram-positive bacteria, gemma 0.6~0.9 × 1.0~
1.5 microns, ellipse arrives column, and central or slightly inclined positioned at thallus, thallus does not expand after sporulation.Bacterium colony rough surface is impermeable
It is bright, it is dirty white or yellowish, when growing in liquid medium, wrinkle mould, aerobic bacteria is commonly formed.Easily bred in withered grass leaching juice.
The subtilin of generation, polymyxins, nystatin, gramicidins isoreactivity object during bacillus subtilis thalli growth
Matter, these active materials have apparent inhibiting effect to the conditioned pathogen of pathogenic bacteria or autogenous infection.Bacillus subtilis
Free oxygen in consumption enteron aisle rapidly, causes enteron aisle hypoxemia, promotes to grow beneficial to anaerobic bacteria, inhibits other pathogenic bacteria raw indirectly
It is long.The growth and development of animal (human body) immune organ is stimulated, cellular immunity and humoral immune function are enhanced, improves herd immunity
Power.Bacillus subtilis thallus itself synthesizes the enzymes such as alpha-amylase, protease, lipase, cellulase.Can synthesize niacin and
A variety of B family vitamins.It is good in gemma state stability inferior, it can resistance to oxidation；Anti-extrusion；High temperature resistant, the long-term resistance to 60 DEG C of high temperature of energy,
It can survive at a temperature of 120 DEG C 20 minutes；Acid and alkali-resistance is able to maintain activity in acidic stomach environment, can attacking with resistance to saliva and bile
Hit, be in microorganism can 100% through intestine and small intestine viable bacteria.
Bacillus licheniformis (scientific name: Bacillus licheniformis): being Gram-positive thermophilic bacteria.Cell shape
State and the in the shape of a rod, Dan Sheng of arrangement, optimum temperature are 37 DEG C.It may exist with spore form, to resist rugged environment；?
Under good environment, then state presence can be grown.The bacterium can adjust flora imbalance and reach therapeutic purposes, body can be promoted to generate anti-
Bacterium active material kills pathogenic bacteria.Antibacterial substance can be generated, and there is unique biology take oxygen mechanism, can be inhibited
The growth and breeding of pathogenic bacteria.
Lactococcus lactis (Lactococcus lactis, L.lactis): being a kind of prokaryotic micro-organisms, cell it is spherical in shape or
Oval, Gram-positive, amphimicrobian do not produce pod membrane and gemma, and optimum growth temperature is 30 DEG C, answer in the food industry
It is the food-grade microorganisms of generally recognized as safe to humans and animals no pathogenicity with extensive.Cheese is in cow's milk (or skimmed milk, dilute milk
Suitable lactic acid bacteria fermenting agent and renin are added in oil, buttermilk etc., solidifies protein of milk, prepared by sepg whey
Fresh or fermenting-ripening dairy products.The nutritive value of cheese is very high, and main component is water, fat and protein.In cheese
Protein is after everfermentation, due to the decomposition of protease in curdled milk and microorganism, forms peptone, peptide, amino acid etc., be easy to by
Human consumption absorbs, and the digestibility of protein is 96-98% in cheese: calcium, phosphorus content in the cheese content in all food
Be it is highest,
Beer yeast slurry: yeast thallus passes through the product that roller drying is formed, and flavor and taste are good, vitamin, protein
Content is high, can eat, is medicinal, can also be from wherein extraction cromoci, nucleic acid, glutathione, thromboplastinum, coacetylase and three
Adenosine phosphate etc..
This patent products characteristics: 1) prominent CORTEX PHELLODENDRI CHINENSE, RHIZOMA COPTIDIS from Sichuan of China, radix scutellariae, Radix Astragali and lichengermium, Lactococcus lactis energy
The major function type of anti-inflammatory acts on；2) digestion of the prominent a variety of enzymes of beer yeast slurry and the attractant effect of yeast fragrance and right
The coverage of Chinese medicine flavour of a drug acts on.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of production methods of functional microbial fermented feed.
The invention belongs to Animal nutritions and technical field of microbial fermentation.
With CORTEX PHELLODENDRI CHINENSE powder, the RHIZOMA COPTIDIS from Sichuan of China in dregs of beans, fulvic acid powder, Dried Corn Steep Liquor Powder, wheat bran skin, bran powder and Chinese medicine
Powder, radix scutellariae powder, astragalus membranaceus powder, Radix Isatidis powder are fermentation raw material, with bacillus subtilis, bacillus licheniformis, Lactococcus lactis, beer
Brewer yeast mud is beneficial microbe colony.Bacillus subtilis, bacillus licheniformis, Lactococcus lactis are respectively by single colonie point
From, solid seed culture and liquid seeds culture, the seed liquor of three kinds of bacterium and beer yeast slurry are mixed in proportion with fermentation raw material
It closes, by aerobic fermentation, anaerobic fermentation, is passed through filtrated air fermentation and anaerobic fermentation four-stage, it is micro- to obtain a kind of functionality
Biological fermentation feed.
Protein, amino acid, short-chain peptide, organic acid, complex enzyme and antibacterial activity thing rich in product of the present invention
Matter, it is full of nutrition, there is good attractant, it is disease-resistant, protection effect is good, promote cultivated animals growth result obvious.Meanwhile also
Breeding environment can be improved, purified water, be a kind of green, safe and efficient functional microbial fermented feed.
A kind of production method of functional microbial fermented feed, with dregs of beans, fulvic acid powder, Dried Corn Steep Liquor Powder, wheat bran skin,
CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, radix scutellariae powder, astragalus membranaceus powder, Radix Isatidis powder in bran powder and Chinese medicine are fermentation raw material, with withered grass bud
Spore bacillus, bacillus licheniformis, Lactococcus lactis, beer yeast slurry are beneficial microbe colony.Bacillus subtilis, lichens bud
Spore bacillus, Lactococcus lactis pass through single colonie separation, solid seed culture and liquid seeds culture respectively, by the seed of three kinds of bacterium
Liquid and beer yeast slurry mix in proportion with fermentation raw material, by aerobic fermentation, anaerobic fermentation, are passed through filtrated air fermentation and detest
Aerobe fermentation four-stage obtains a kind of functional microbial fermented feed.
By weight, fermentation raw material forms are as follows:
10~30 parts by weight of dregs of beans, 5~15 parts by weight of fulvic acid powder, 10~30 parts by weight of Dried Corn Steep Liquor Powder, wheat bran skin 10
~30 parts by weight, 10~30 parts by weight of bran powder, 1~3 parts by weight of CORTEX PHELLODENDRI CHINENSE powder, 1~3 parts by weight of RHIZOMA COPTIDIS from Sichuan of China powder, radix scutellariae powder 1~
3 parts by weight, 1~3 parts by weight of astragalus membranaceus powder, 1~3 parts by weight of Radix Isatidis powder；Beneficial microbe colony weight ratio are as follows: bacillus subtilis
30~40 parts by weight of bacterium seed liquor, 30~40 parts by weight of bacillus licheniformis seed liquor, 20~30 weight of Lactococcus lactis seed liquor
Measure part, 20~30 parts by weight of beer yeast slurry；The weight ratio of fermentation raw material and beneficial microbe colony be fermentation raw material 100~
120 parts by weight, 30~50 parts by weight of beneficial microbe colony.
Viable count >=10,000,000,000 CFU/ml in bacillus subtilis seed liquor, viable count is in bacillus licheniformis seed liquor
In >=100 hundred million CFU/ml, Lactococcus lactis seed liquor viable count be >=80 hundred million CFU/ml, in beer yeast slurry viable count be >=
100000000000 CFU/ml.
The mass water content of each substance in fermentation raw material is dry with Dou Po≤10%, fulvic acid Fen≤10%, corn pulp
Powder≤10%, wheat bran skin≤10%, rice chaff powder≤10%, CORTEX PHELLODENDRI CHINENSE powder≤10%, RHIZOMA COPTIDIS from Sichuan of China powder≤10%, Huang Qin powder≤
10%, Radix Astragali Fen≤10%, Radix Isatidis Fen≤10%.Gross mass Han Shui Liang≤10% of the fermentation raw material.
By weight, fermentation raw material forms are as follows: 20 parts by weight of dregs of beans, 10 parts by weight of fulvic acid powder, Dried Corn Steep Liquor Powder 20
Parts by weight, 20 parts by weight of wheat bran skin, 20 parts by weight of bran powder, 2 parts by weight of CORTEX PHELLODENDRI CHINENSE powder, 2 parts by weight of RHIZOMA COPTIDIS from Sichuan of China powder, yellow celery powder 2
Parts by weight, 2 parts by weight of astragalus membranaceus powder, 2 parts by weight of Radix Isatidis powder；The weight ratio of beneficial microbe colony are as follows: bacillus subtilis strain
Sub- 30 parts by weight of liquid, 30 parts by weight of bacillus licheniformis seed liquor, 20 parts by weight of Lactococcus lactis seed liquor, beer yeast slurry 20
Parts by weight；The weight ratio of fermentation raw material and beneficial microbe colony is 100 parts by weight of fermentation raw material, 50 weight of beneficial microbe colony
Measure part.
Specifically includes the following steps:
(1) preparation of fermentation raw material:
Fermentation raw material: dregs of beans, fulvic acid powder, Dried Corn Steep Liquor Powder, wheat bran skin, bran powder is crushed to 30 mesh respectively；Will in
CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, yellow celery powder, astragalus membranaceus powder, Radix Isatidis powder in medicinal material, are crushed to 100 mesh respectively；It will in required ratio
CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, yellow celery powder, astragalus membranaceus powder, Radix Isatidis powder in Chinese medicine are sufficiently mixed, by the beans in fermentation raw material
The dregs of rice, fulvic acid powder, Dried Corn Steep Liquor Powder, wheat bran skin, bran powder are sufficiently mixed, then the two are sufficiently mixed spare；
(2) preparation of beneficial microbe colony:
The preparation of bacillus subtilis seed liquor:
Single colonie separation: activated bacillus subtilis slant strains are being filled into the glucose beef extract egg newly prepared
It is coated on the culture dish of white peptone culture medium, 30 DEG C in constant incubator, cultivates within 24 hours, obtain bacillus subtilis single bacterium
Planting；
The flat bottle seed culture of solid eggplant shape: bacillus subtilis single colonie seed is filling the glucose beef extract newly prepared
It is inoculated in the flat bottle of 200ml eggplant shape of peptone culture medium, 30 DEG C in constant incubator, cultivates within 24 hours, obtain withered grass gemma
Bacillus solid seed；
Fermentor seed culture: the flat bottle seed of bacillus subtilis eggplant shape is calculated as 200 kilograms in mass fraction ratio
Liquid accesses the inoculum concentration of the flat bottle seed of 1 eggplant shape, is inoculated into fermentation tank culture medium (700 kg liquids, 3~4 eggplants of access
The flat bottle seed of shape), it is cultivated, obtains bacillus subtilis seed.
Fermentation of bacillus subtilis tank seed culture medium is by weight percentage are as follows: glucose 0.5%~1.0%, corn
Paste dry powder 3.0%~3.5%, ammonium sulfate 0.1%~0.2%, magnesium sulfate 0.1%~0.2%, dipotassium hydrogen phosphate 0.1%~
0.2%, surplus is water, pH6.8~7.0；Sterilising conditions: 115~121 DEG C of temperature, 0.08~0.10MPa of pressure, the time 18~
20min；Condition of culture: inoculum concentration 10~20%；Tank presses 0.01~0.10MPa, air quantity 100:100~100:200vvm, revolving speed
30 DEG C~32 DEG C of 100~200rpm, temperature；48~64h of time.Viable bacteria containing bacillus subtilis in bacillus subtilis seed liquor
>=100 hundred million/ml of number；
The preparation of bacillus licheniformis seed liquor:
The single colonie separation of bacillus licheniformis, the flat bottle seed culture of solid eggplant shape, fermentor seed culture and withered grass bud
Spore bacillus is identical.
Viable count containing bacillus subtilis >=10,000,000,000/ml in bacillus licheniformis seed liquor；
The preparation of Lactococcus lactis seed liquor:
Single colonie separation: activated Lactococcus lactis slant strains are being filled into the glucose proteins peptone beef newly prepared
It is coated on the culture dish of cream yeast extract medium, 35 DEG C in constant incubator, cultivates within 48 hours, obtain plant single bacterium planting
Son；
The flat bottle seed culture of solid eggplant shape: Lactococcus lactis single colonie seed is filling glucose proteins peptone beef extract yeast
It is inoculated in the flat bottle of 200ml eggplant shape of cream culture medium, 35 DEG C in constant incubator, cultivates within 48 hours, it is solid to obtain Lactococcus lactis
Body seed；
Fermentor seed culture: the flat bottle seed of Lactococcus lactis eggplant shape is calculated as 200 kilograms of liquid in mass fraction ratio
Body accesses the inoculum concentration of the flat bottle seed of 1 eggplant shape, is inoculated into fermentation tank culture medium (700 kg liquids, 3~4 eggplant shapes of access
Flat bottle seed), it is cultivated, obtains Lactococcus lactis seed liquor；
Lactococcus lactis fermentor seed culture medium is by weight percentage are as follows: glucose 3.0~7.0%, yeast powder
1.0%~2.0%, Dried Corn Steep Liquor Powder 1.0%~2.0%, ammonium sulfate 0%~0.1%, magnesium sulfate 0%~0.1%, phosphoric acid hydrogen
Dipotassium 0%~0.1%, surplus are water, pH6.4~6.6；Sterilising conditions: 115~121 DEG C of temperature, pressure 0.08~
0.10MPa, 18~20min of time；Condition of culture: inoculum concentration 10~20%；0.01~0.10MPa of tank pressure, air quantity 100:30~
100:70vvm, 30~70rpm of revolving speed, 33 DEG C~35 DEG C of temperature；48~64h of time (being stationary culture after 24 hours).Plant cream
Viable count containing plant >=8,000,000,000/ml in bacillus seed liquor.
Beer yeast slurry: beer yeast slurry is bought from Shenyang Co., Ltd, Yanjing Brewery.
By obtained bacillus subtilis seed liquor, bacillus licheniformis seed liquor, Lactococcus lactis seed liquor；By required
Ratio is abundant by bacillus subtilis seed liquor, bacillus licheniformis seed liquor, Lactococcus lactis seed liquor and beer yeast slurry
Mixing for standby use；
(3) the solid fermentation stage:
Solid fermentation process is: by aerobic fermentation, anaerobic fermentation, being passed through filtrated air fermentation and four ranks of anaerobic fermentation
Section, obtains a kind of functional microbial fermented feed, specifically:
It first will be withered in required ratio with sodium hydroxide adjustment pH value to 6.5~7.0 by uniformly mixed fermentation raw material
Careless bacillus seed liquor, bacillus licheniformis seed liquor, fermented glutinous rice yeast bacterium seed liquor, Lactococcus lactis seed liquor and beer ferment
Female mud mixing, is inoculated into prepared fermentation raw material, and the fermenter with ventilation and breathing equipment is encased in after mixing well
Or in ventilation and breathing equipment fermentation bag, compacting is covered tightly, and is placed into solid fermentation workshop and is carried out solid fermentation；
1) aerobic solids fermentation stage: (the 1st day) 0~24 hour, standing for fermentation, room temperature are controlled 28 DEG C~38
℃；
2) anaerobic solids fermentation stage: (the 2nd day) 24~48 hours, standing for fermentation, room temperature are controlled 36 DEG C~38
℃；
3) it is passed through filtrated air fermentation stage: (the 3rd, 4 day) 48~96 hours, to the fermenter or band for having breathing equipment
Have in breathing equipment fermentation jumbo bag, filtrated air is injected by air pump, ventilation oxygen supply air quantity is 0.1~0.3V/Vmin, Indoor Temperature
Degree control is at 28 DEG C~38 DEG C；
4) anaerobism (standing forms gemma and metabolite) fermentation stage: (5,6 days) 120~144 hours stop to band
There is the fermenter of ventilation and breathing equipment or with air is passed through in ventilation and breathing equipment fermentation bag, room temperature is controlled 28
DEG C~38 DEG C；
(4) low temperature drying crushing stage:
After solid fermentation, fermentation material is transferred on negative-pressure fluidized-bed, carries out low temperature drying, inlet temperature control exists
80~90 DEG C, outlet temperature is controlled at 40~50 DEG C, and fermentation material 12~20min of residence time in fluidized bed is dried to aqueous
Amount is 10~16%, is crushed to 70~90 mesh using high speed pulverizing mill, obtains a kind of functional microbial fermented feed.
Through detecting: product crude protein 30~33%, crude fibre 11.0~15.0%, relies molten protein 10 .0~12.0% of acid
Propylhomoserin 3.1~3.3%, mannosan 3.0~7.0%, VBN50~60mg/100g, ash content 9.0~11.0%, total acidity 2.1
~2.3%, 1800~2000U/g of proteinase activity, 10.0~12.0mm of antibacterial circle diameter, ash content 13~18%, water content
11.0~13.0%.
Its preferred steps are as follows:
(1) preparation of the raw material to ferment:
By fermentation raw material: dregs of beans, fulvic acid powder, Dried Corn Steep Liquor Powder, wheat bran skin, bran powder are crushed to 30 mesh respectively, will in
CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, yellow celery powder in medicinal material are crushed to 60 mesh；In required ratio by the CORTEX PHELLODENDRI CHINENSE powder in Chinese medicine, river
Coptis powder, yellow celery powder, astragalus membranaceus powder, Radix Isatidis powder are sufficiently mixed, by fermentation raw material dregs of beans, fulvic acid powder, Dried Corn Steep Liquor Powder,
Wheat bran skin, bran powder are sufficiently mixed, then the two are sufficiently mixed spare；
(1) preparation of beneficial microbe colony:
Fermentation of bacillus subtilis tank seed culture medium is by weight percentage are as follows: glucose 1.0%, Dried Corn Steep Liquor Powder
3.0%, ammonium sulfate 0.2%, magnesium sulfate 0.2%, dipotassium hydrogen phosphate 0.2%, surplus are water, pH6.8；Sterilising conditions: temperature 121
DEG C, pressure 0.10MPa, time 20min；Condition of culture: inoculum concentration 10%；Tank presses 0.5MPa, air quantity 100:150vvm, revolving speed
100rpm, 32 DEG C of temperature；Time 48h.Viable count containing bacillus subtilis >=10,000,000,000/ml in bacillus subtilis seed liquor；
The preparation of bacillus licheniformis seed liquor:
The single colonie separation of bacillus licheniformis, the flat bottle seed culture of solid eggplant shape, fermentor seed culture and withered grass bud
Spore bacillus is identical.Viable count containing bacillus subtilis >=10,000,000,000/ml in bacillus licheniformis seed liquor；
Lactococcus lactis fermentor seed culture medium is by weight percentage are as follows: glucose 5.0%, yeast powder 2.0%,
Dried Corn Steep Liquor Powder 1.0%, ammonium sulfate 0.1%, magnesium sulfate 0.1%, dipotassium hydrogen phosphate 0.1%, surplus are water, pH6.4；Sterilizing
Condition: 115 DEG C of temperature, pressure 0.08MPa, time 18min；Condition of culture: inoculum concentration 10%；Tank presses 0.05MPa, air quantity 100:
30vvm, revolving speed 30rpm, 35 DEG C of temperature；Time 48h (being stationary culture after 24 hours).It is living containing plant in Lactococcus seed liquor
Bacterium number >=8,000,000,000/ml.
It will obtain bacillus subtilis seed liquor, bacillus licheniformis seed liquor, lactic acid goal coccus seed liquor；By required
Ratio is abundant by bacillus subtilis seed liquor, bacillus licheniformis seed liquor, Lactococcus lactis seed liquor and beer yeast slurry
Mixing for standby use；
Fermentation raw material that first will be uniformly mixed adjusts pH value to 6.5 with sodium hydroxide, in required ratio by withered grass gemma
Bacillus seed liquor, bacillus licheniformis seed liquor, fermented glutinous rice yeast bacterium seed liquor, Lactococcus lactis seed liquor and beer yeast slurry are mixed
It closes, is inoculated into prepared fermentation raw material, the fermenter with ventilation and breathing equipment is encased in after mixing well or has
In ventilation and breathing equipment fermentation bag, compacting is covered tightly, and is placed into solid fermentation workshop and is carried out solid fermentation；
1) aerobic solids fermentation stage: (the 1st day) 24 hours, standing for fermentation, room temperature are controlled at 37 DEG C；
2) anaerobic solids fermentation stage: (the 2nd day) 24-48 hours, standing for fermentation, room temperature are controlled at 38 DEG C；
3) be passed through filtrated air fermentation stage: (the 3rd, 4 day) 48-96 hours passes through gas into fermenter or fermentation bag
Pump injection filtrated air, ventilation oxygen supply air quantity is 0.3V/Vmin, and room temperature is controlled at 38 DEG C；
4) anaerobism (standing forms gemma and metabolite) fermentation stage (5,6 days) 96-144 hours stop to logical
The fermenter of gas and breathing equipment or with air is passed through in ventilation and breathing equipment fermentation bag, room temperature is controlled at 38 DEG C；
The ventilation refers to lower end air inlet, the upper end of the other side with the fermenter of breathing equipment or with ventilation and breathing equipment fermentation bag
The device of outlet.
(4) low temperature drying crushing stage:
After solid fermentation, fermentation material is transferred on negative-pressure fluidized-bed, carries out low temperature drying, inlet temperature control exists
85 DEG C, outlet temperature is controlled at 45 DEG C, fermentation material residence time 16min in fluidized bed, and being dried to water content is 13%, then is passed through
It crosses high speed pulverizing mill and is crushed to 80 mesh, obtain a kind of functional microbial fermented feed.
Through detecting: product crude protein 33%, the molten protein 12 .0% of acid, crude fibre 15.0%, lysine 3.3%, sweet dew are poly-
Sugared 5.0%, VBN 60mg/100g, ash content 10.0%, total acidity 2.3%, proteinase activity 2000U/g, antibacterial circle diameter
10.0mm, ash content 10%, water content 12.0%, and contain bacillus subtilis >=1,000,000,000 CFU/g, bacillus licheniformis >=10
Hundred million CFU/g, Lactococcus lactis >=1.0 hundred million CFU/g, beer yeast live bacteria number are >=1.0 hundred million CFU/g.This product is full of nutrition, prevents
Sick effect is good, promotes cultivated animals growth result obvious.Breeding environment can also be improved, purified water, be a kind of green high quality
Functional microbial fermented feed.
Protein, amino acid, short-chain peptide, organic acid, complex enzyme and antibacterial activity thing rich in product of the present invention
Matter, it is full of nutrition, there is good attractant, it is disease-resistant, protection effect is good, promote cultivated animals growth result obvious.Meanwhile also
Breeding environment can be improved, purified water, be a kind of green, safe and efficient functional microbial fermented feed.
The beneficial effects of adopting the technical scheme are that
The present invention uses fermentation raw material sources specific, dregs of beans, fulvic acid powder, Dried Corn Steep Liquor Powder, wheat bran skin, bran powder
And CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, yellow celery powder, astragalus membranaceus powder, Radix Isatidis powder in Chinese medicine；
(1) fermentation raw material is from a wealth of sources, and the various performances of raw material are stablized, the functionality and its definite effect that have, purity is high,
Solubility is good, for example, beer yeast slurry, has multiple advantages, and also has the tempting smell of fragrance, has food calling to cultivated animals
Feel, here, the slight bitter taste for coverage Chinese herbal medicine taken.The five kinds of Chinese herbal medicines used all have antibacterial activity, crush 100 mesh
Afterwards, then carry out the enhancing of fermentation period pharmacological property and persistently, for the diseases such as digestive system inflammation have good therapeutic effect.
(2) fermenting microbe source of the present invention is specific, with bacillus subtilis, bacillus licheniformis, lactic acid cream
Coccus, fermented glutinous rice yeast bacterium mud are beneficial microbe colony, and various bacterium raw material effects are definite, the active height of fermentation unit, cut antimicrobial spectrum
There is good antibacterial effect to Gram-negative bacteria extensively, there is good Antidiarrheic effect；It can also promote intestinal health
Effect inhibits harmful bacteria growth.And the enzyme secretion that can stimulate digestion, promotion appetite, promotion digestion, promotion daily gain；Have anti-very well
Oxidative function, phenolic substances have scavenging effect to the oxygen radical in body cell, improve animal body immunity and promotion
Growth of animal effect.
(3) present invention have fermentation raw material source be it is specific, simple production process, with short production cycle, good product quality is answered
The features such as good with effect.This product is added in the feed in precious marine product, aquatic products livestock and poultry cultivation, can be prevented and cured diseases, improve at
Motility rate improves immunity, improves the speed of growth, improves quality, can improve breeding environment, purify water, can effectively press down
The growth and insecticidal action of Penicillium notatum and black-koji mould in feed processed.This product be it is a kind of it is green, safe and efficient, without pair make
With, the environmentally friendly of noresidue, functional aquatic products livestock and poultry feed addictive, have a vast market foreground.
Specific embodiment
Various embodiments of the present invention use raw material: fulvic acid is bought from Shanghai humic acid biochemical corp；CORTEX PHELLODENDRI CHINENSE, river are yellow
Company, radix scutellariae, Radix Astragali, Radix Isatidis are commercially；Dried Corn Steep Liquor Powder is bought from Shandong He Banshan Biotechnology Co., Ltd；
Beer yeast slurry is bought from Shenyang Co., Ltd, Yanjing Brewery.
Various embodiments of the present invention use strain: the Bacillus subtilis subspecies of bacillus subtilis
(Bacillussubtilissubsp.subtilis), CICC number is 20822 and bacillus licheniformis is micro- from Chinese industrial
Biological inoculum preservation administrative center preservation purchase.
Various embodiments of the present invention are using Lactococcus lactis by Shenyang Inst. of Applied Ecology, Chinese Academy of Sciences agricultural microorganism
Technology groups are given.
Fermentation ventilatory capacity (V/V.min): (usually per minute) gas volume of round is passed into the unit time.
Bacteriostatic activity: the substance of certain inhibition tool bacterium growths can be secreted, forming tool bacterium in the periphery of bacterial colonies cannot give birth to
Long inhibition zone.
Fermenter with ventilation and breathing equipment: Bo Shan biotech firm patented technology production
A kind of 201420792557.9 utility model of feed fermentation device
Fermentation bung and 201620323196.2 utility model of fermenter
Embodiment 1
(1) fulvic acid powder and CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, radix scutellariae powder, astragalus membranaceus powder, color in Radix Isatidis powder formula combination,
The determination of smell, inhibition zone:
By 5~15 parts by weight of fulvic acid powder, 1~3 parts by weight of CORTEX PHELLODENDRI CHINENSE powder, 1~3 parts by weight of RHIZOMA COPTIDIS from Sichuan of China powder, radix scutellariae powder 1
~3 parts by weight, 1~3 parts by weight of astragalus membranaceus powder, 1~3 parts by weight of Radix Isatidis powder are taken out spare.
Fulvic acid powder, CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, radix scutellariae powder, astragalus membranaceus powder, Radix Isatidis powder are crushed to 100 mesh respectively, pressed
It is uniformly mixed according to ratio spare.
Each combination is put into water 1000ML water with 1 gram of powder respectively, that is, dilutes 1000 times, then place 1 hour after boiling,
It is spare after temperature is down to room temperature；Test organisms inhibits: Escherichia coli.
Medicine sensitization test is done with cylinder plate method, and observes the results such as each combined color and smell, inhibition zone.Test is set
Meter:
3 horizontal colors of functional 62 combinations of factors of kind, smell, the experimental design of inhibition zone and test result are such as
The following table 1；
Table 1, fulvic acid powder, CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, astragalus membranaceus powder, astragalus membranaceus powder, color in Radix Isatidis powder formula combination,
Smell, inhibition zone test
Preferred result as can be seen from Table 1: 10 parts by weight of fulvic acid powder, 2 parts by weight of CORTEX PHELLODENDRI CHINENSE powder, 2 weight of RHIZOMA COPTIDIS from Sichuan of China powder
Part, yellow 2 parts by weight of celery powder, 2 parts by weight of astragalus membranaceus powder, 2 parts by weight of Radix Isatidis powder, color is good, smell is good, inhibition zone is big, antibacterial knot
Fruit shows there is good inhibitory effect to Gram-negative bacterium.Bacillus subtilis, bacillus licheniformis are gram dye
Color is positive, Escherichia coli Gram-negative.
Embodiment 2:
The confirmation of fermentation of bacillus subtilis tank seed culture based formulas and its fermentation condition
Bacillus subtilis (Bacillus subtilis) bacterial micro-organism, (main decomposition albumen acts on synthesizing amino acid
And peptide matters).Single colonie purifying and preferred, the flat bottle seed culture of solid eggplant shape and fermentor seed culture concrete operation step
As follows, bacillus subtilis is fast with reproduction speed, high temperature resistant, acid and alkali-resistance, anti-extrusion, stability are good, have a wide range of application
Feature.
Single colonie separation: activated bacillus subtilis slant strains are being filled into the glucose beef extract egg newly prepared
It is coated on the culture dish of white peptone culture medium, 30 DEG C in constant incubator, cultivates within 24 hours, obtain bacillus subtilis single bacterium
Planting；
The flat bottle seed culture of solid eggplant shape: bacillus subtilis single colonie seed is filling the glucose beef extract newly prepared
It is inoculated in the flat bottle of 200ml eggplant shape of peptone culture medium, 30 DEG C in constant incubator, cultivates within 24 hours, obtain withered grass gemma
Bacillus solid seed；
Fermentor seed culture: the flat bottle seed of bacillus subtilis eggplant shape is calculated as 200 kilograms in mass fraction ratio
Liquid accesses the inoculum concentration of the flat bottle seed of 1 eggplant shape, is inoculated into fermentation tank culture medium (700 kg liquids, 3~4 eggplants of access
The flat bottle seed of shape), it is cultivated, obtains bacillus subtilis seed.
The movement of test under microscope bacterium thallus is active, healthy and strong, neatly, no miscellaneous bacteria, free from extraneous odour, the abnormal phenomenon such as pollution-free,
Microscopy viable count obtains to be used in mixed way up to 10,000,000,000/ml (calculating bacterium number using counting method of blood cell).
1, fermentation of bacillus subtilis tank seed culture medium and condition of culture test
A) fermentation of bacillus subtilis tank seed-test:
Experimental design is that 4 factor 3 is horizontal, experimental design and test result such as the following table 2, table 3:
Table 2, fermentation of bacillus subtilis tank seed culture medium formula test
| The factor/level | Ⅰ | Ⅱ | Ⅲ |
| Glucose (g/1000ml) | 5 | 7.5 | 10 |
| Dried Corn Steep Liquor Powder (g/1000ml) | 30 | 32.5 | 35 |
| Ammonium sulfate (g/1000ml) | 1 | 1.5 | 2 |
| Magnesium sulfate (g/1000ml) | 1 | 1.5 | 2 |
| Dipotassium hydrogen phosphate (g/1000ml) | 1 | 1.5 | 2 |
| Counting alive microbial result | 10.9*10 | 10.6*10 | 10.3*10 |
B) bacillus subtilis seed tank culture condition test
Experimental design is that factor 3 is horizontal, experimental design and test result such as the following table 3: part test
Table 3, fermentation of bacillus subtilis tank seed culture condition
By weight percentage by the determining fermentation of bacillus subtilis tank seed culture medium of the test result of table 2, table 3
Are as follows: glucose 0.5%, Dried Corn Steep Liquor Powder 3.0%, ammonium sulfate 0.1%, magnesium sulfate 0.1%, dipotassium hydrogen phosphate 0.1%, surplus
For water, pH7.0；Sterilising conditions: 121 DEG C of temperature, pressure 0.10MPa, time 20min；Condition of culture: inoculum concentration 10%；Tank pressure
0.05MPa, air quantity (air) 100:200vvm, revolving speed 200rpm, 32 DEG C of temperature；Time 48h.
Embodiment 3
Best fermentation raw material proportion, fermentation etc. are tested in solid fermentation process:
By weight, fermentation raw material forms are as follows: 10~30 parts by weight of dregs of beans, 5~15 parts by weight of fulvic acid powder, corn
10~30 parts by weight of paste dry powder, 10~30 parts by weight of wheat bran skin, 10~30 parts by weight of bran powder, 1~3 parts by weight of CORTEX PHELLODENDRI CHINENSE powder,
1~3 parts by weight of RHIZOMA COPTIDIS from Sichuan of China powder, 1~3 parts by weight of radix scutellariae powder, 1~3 parts by weight of astragalus membranaceus powder, 1~3 parts by weight of Radix Isatidis powder；
Beneficial microbe colony weight composition are as follows: 30~40 parts by weight of bacillus subtilis seed liquor, bacillus licheniformis
30~40 parts by weight of seed liquor, 20~30 parts by weight of Lactococcus lactis seed liquor, 20~30 parts by weight of beer yeast slurry；
The weight ratio of fermentation raw material and beneficial microbe colony is 80~110 parts by weight of fermentation raw material, beneficial microbe bacterium
30~50 parts by weight of group.
1) aerobic solids fermentation stage: (the 1st day) 0~24 hour, standing for fermentation, room temperature are controlled 28 DEG C~38
℃；
2) anaerobic solids fermentation stage: (the 2nd day) 24~48 hours, standing for fermentation, room temperature are controlled 36 DEG C~38
℃；
3) it is passed through filtrated air fermentation stage: (the 3rd, 4 day) 48~96 hours, to the fermentation with ventilation and breathing equipment
Bucket has in ventilation and breathing equipment fermentation bag, and air pump injects filtrated air, and ventilatory capacity is 0.1~0.3V/V min, interior
Temperature is controlled at 28 DEG C~38 DEG C；
4) anaerobism (standing forms gemma and metabolite) fermentation stage (5,6 days) 96~144 hours stop with logical
The fermenter of gas and breathing equipment or with being passed through air in ventilation and breathing equipment fermentation bag, room temperature control 28 DEG C~
38℃；
Different ratios of raw materials different moisture content influences experimental design to fermentation temperature and its results are shown in Table 4, table 5.
Table 4, different ratios of raw materials different moisture content influence experimental design to fermentation temperature
The influence of table 5, different ratios of raw materials different moisture content to fermentation temperature
From table 4, that table 5 obtains interpretation of result is as follows: in 9 test combinations, 1,2,3 group of formula are fermented, and 2 combinations are matched
Side be it is best, ferment warm fast, persistently, smell might as well, color, no miscellaneous bacteria: 4,5,6, combination formula take second place, sequentially
It is 5,4,6；7, it 8,9 combines third.Entering and is extended to this scheme, water content is added according to 100:60, and fermentation can also carry out,
But moisture is excessively high, has been easy miscellaneous bacteria, and the drying to later product, has caused energy waste.It is analyzed, is tied by this
Fruit is, by weight, the composition of fermentation raw material are as follows: 20 parts by weight of dregs of beans, 10 parts by weight of fulvic acid powder, 20 weight of Dried Corn Steep Liquor Powder
Measure part, 20 parts by weight of wheat bran skin, 20 parts by weight of bran powder, 2 parts by weight of CORTEX PHELLODENDRI CHINENSE powder, 2 parts by weight of RHIZOMA COPTIDIS from Sichuan of China powder, 2 weight of radix scutellariae powder
Measure part, 2 parts by weight of Radix Astragali, 2 parts by weight of Radix Isatidis powder；
The weight of beneficial microbe colony forms are as follows: 30 parts by weight of bacillus subtilis seed liquor, bacillus licheniformis kind
Sub- 30 parts by weight of liquid, 20 parts by weight of Lactococcus lactis seed liquor, 20 parts by weight of beer yeast slurry；
The weight ratio of fermentation raw material and beneficial microbe colony is 100 parts of fermentation raw material, 50 parts of beneficial microbe colony.
1) aerobic solids fermentation stage: (the 1st day) 0-24 hours, standing for fermentation, room temperature control at 37 DEG C to be best,
Main growth bacterium is bacillus subtilis and lichengermium, and secondary growth bacterium is saccharomycete, can be made full use of in fermenter
Free oxygen carries out aerobic fermentation, gives off carbon dioxide, and by breathing equipment, excludes, does not generate carbon dioxide poisoning phenomenon；
2) anaerobic solids fermentation stage: (the 2nd day) 24-48 hours, standing for fermentation, room temperature control is at 38 DEG C for most
Good, main to grow bacterium for Lactococcus lactis and saccharomycete, Lactococcus lactis, saccharomycete make full use of the residual ionization in fermenter
It oxygen and ferments under anaerobic, Lactococcus well-grown, and lactic acid is generated, saccharomycete can be grown, in anaerobic condition
Under, generate metabolite and its alcohol and fragrant aroma.It slightly gives off carbon dioxide, by breathing equipment, excludes, do not generate two
Carbon monoxide poisoning phenomenon；
3) be passed through filtrated air fermentation stage: (the 3rd, 4 day) 48-96 hours passes through gas into fermenter or fermentation bag
Pump injection filtrated air, ventilation oxygen supply air quantity are 0.3V/Vmin, room temperature control at 38 DEG C to be best, it is main to grow bacterium and be
Bacillus subtilis and lichengermium, secondary growth bacterium are saccharomycete and Lactococcus lactis, can make full use of injection fermenter
Interior free oxygen carries out aerobic fermentation, and bacillus subtilis and lichengermium are quickly bred, saccharomycete and Lactococcus lactis
Liquid is slightly proliferated, the carbon dioxide of exhalation, by breathing equipment, is excluded, is not generated in carbon dioxide；
4) anaerobism (standing forms gemma and metabolite) fermentation stage (5,6 days) 120 hours stop to breathing
The fermenter of device or with air is injected in breathing equipment fermentation jumbo bag, room temperature control is best, this stage at 38 DEG C
It is most preferably accumulated the phase for fermentating metabolism product, breaks down proteins are at amino acid and small peptide nitrogen metabolism in secondary formation, point of cellulose family
Solution is formed herein at starch and carbohydrate and its energetic supersession, and most importantly Bacillus can become gemma in this stage, is made
It, contains a large amount of Bacillus in fermentation material.
The condition Selection experiment of best dries pulverizing:
After solid fermentation, fermentation material is transferred on negative-pressure fluidized-bed, carries out low temperature drying, inlet temperature control exists
80~90 DEG C, outlet temperature is controlled at 40~50 DEG C, and fermentation material 12~20min of residence time in fluidized bed is dried to aqueous
Amount is 10~16%, is crushed to 70~90 mesh using high speed pulverizing mill, obtains a kind of functional microbial fermented feed.
The influence of table 6 different stream temperatures and time to fermentation material water content and microbiological indicator
As can be seen from Table 6:
After solid fermentation, fermentation material is transferred on negative-pressure fluidized-bed, carries out low temperature drying, inlet temperature control exists
85 DEG C, outlet temperature is controlled at 45 DEG C, fermentation material residence time 16min in fluidized bed, and being dried to water content is 13%, then is passed through
It crosses high speed pulverizing mill and is crushed to 80 mesh, obtain a kind of functional microbial fermented feed.
The Selection experiment of the technical indicator of functional microbial fermented feed:
Measurement of the solid fermentation with raw material proportioning product guarantee value:
The range of solid fermentation different ratios of raw materials product guarantee value exists: product crude protein 30~33%, the molten albumen of acid
10.0~12.0%, 11.0~15.0% lysine 3.1~3.3% of crude fibre, mannosan 3.0~7.0%, VBN50~
60mg/100g, ash content 9.0~11.0%, total acidity 2.1~2.3%, 1800~2000U/g of proteinase activity, inhibition zone are straight
10.0~12.0mm of diameter, ash content 13~18%, water content 11.0~13.0%.
The measurement result of table 7, solid fermentation different ratios of raw materials product guarantee value
As can be seen from Table 7: product crude protein 33%, the molten protein 12 .0% of acid, crude fibre 15.0%, lysine 3.3%,
Mannosan 5.0%, VBN 60mg/100g, ash content 12.0%, total acidity 2.3%, proteinase activity 2000U/g, inhibition zone are straight
Diameter 10.0mm, ash content 10%, water content 12.0%, and contain bacillus subtilis >=1,000,000,000 CFU/g, bacillus licheniformis
>=10 hundred million CFU/g, Lactococcus lactis >=1.0 hundred million CFU/g, beer yeast live bacteria number >=1.0 hundred million CFU/g.This product nutrition is rich
Richness, protection effect is good, promotes cultivated animals growth result obvious.Breeding environment can also be improved, purified water, be a kind of green
Good functional microbial fermented feed.
Embodiment 4
This patent product functionality fermentative feedstuff of microbe that embodiment 3 obtains is added in the egg feedstuff of 28 week old and is answered
With.
Firstly, carrying out preliminary experiment, the preliminary experiment phase 28 days, fed 28 days using basic egg fowl material, preliminary experiment terminates
Afterwards, start formal test, carried out according to above-mentioned experimental design, and carry out the record statistics of experimental data:
Table 8, functional microbial fermented feed are to the experimental design of the laying rate of laying hen and the influence of feedstuff-egg ratio
Conventional microbiological fermented feed is the production of Shenyang Bo Shanyingsheng Bioisystech Co., Ltd, the name of an article are as follows: health times stick (egg
Chicken use)
Table 9, functional microbial fermented feed are to the laying rate of laying hen and the influence of feedstuff-egg ratio
Note: experimental period is 30 days.
As shown in Table 9,5% equivalent substitution addition functional microbial hair is carried out in the egg fowl material of the laying hen of 28 week old
Its laying rate can be improved in ferment feed, reduces its feedstuff-egg ratio, compared with the control group, 1 laying rate increase rate 1.26% of test group,
Feedstuff-egg ratio rate of descent 3.93%；2 laying rate increase rate 1.05% of test group, feedstuff-egg ratio rate of descent 6.82%.As it can be seen that equivalent substitution
Functional fermented feed is added, bacteriosis can be inhibited, improves intestinal health, promotes growth, extends egg-laying peak.From
Egg is seen in appearance；Shell is smooth, and eggshell hardness is promoted, and eggshell thickens, and doped calcium increases.Yolk consolidation is bright, dense albumen
Density increases, and hangh unit increases.Improve the quality and flavor of egg simultaneously.Economic benefit can be increased with energy-saving and emission-reduction.
Embodiment 5
This patent product functionality fermentative feedstuff of microbe that embodiment 3 is obtained, does on broiler chicken and is averaged to broiler chicken
The influence of daily gain and feedstuff-meat ratio is tested.
Firstly, carrying out preliminary experiment, in 5 Day-old Broiler Chickens, fed 7 days using basic poultry material, to 12 ages in days, preliminary experiment
After, start formal test, carried out according to above-mentioned experimental design, and carries out the record statistics of experimental data:
The experimental design that table 10, functional microbial fermented feed influence the average daily gain of broiler chicken and feedstuff-meat ratio
| Control group | Poultry material |
| Test group 1 | Poultry material -5% dosage of poultry material (weight percent)+5% dosage of conventional microbiological fermented feed |
| Test group 2 | Poultry material -5% dosage of poultry material (weight percent)+5% dosage of functional microbial fermented feed |
Conventional microbiological fermented feed is the production of Shenyang Bo Shanyingsheng Bioisystech Co., Ltd, the name of an article are as follows: health times stick (meat
Chicken use)
Table 11, functional microbial fermented feed influence the average daily gain of broiler chicken and feedstuff-meat ratio
Note: experimental period is 30 days.
As shown in Table 11, the equivalent substitution addition addition of progress 5% is functional micro- in the poultry material of the broiler chicken of 12 ages in days
Biological fermentation feed can be such that the average daily gain of broiler chicken increases, and feedstuff-meat ratio reduces, and compared with the control group, test group 1 is averaged day
Weight gain increases by 3.76%, feedstuff-meat ratio rate of descent 4.05%；2 average daily gain increment rate 5.61% of test group, feedstuff-meat ratio rate of descent
6.26%, equal otherness is significant.As it can be seen that equivalent substitution adds functional fermented feed, feed conversion rate can be improved, improve broiler chicken
Intestinal microflora, to a certain degree prevention intestines problem and respiratory disease, feed intake is restored after facilitating chicken proventriculitis, can have
Effect improves the production performance and Slaughter of broiler chicken, achieves energy-saving and emission reduction purposes, and increases economic benefit.
Embodiment 6:
This patent product functionality fermentative feedstuff of microbe that embodiment 3 is obtained, average daily gain, diarrhea in piglet
The influence application test of rate and death and culling rate.
Firstly, carrying out preliminary experiment using baby pig feedstuff, preliminary experiment is 10 days, after preliminary experiment, starts formal examination
It tests, is carried out according to above-mentioned experimental design, and carry out the record statistics of experimental data:
The influence test of table 12, functional microbial fermented feed to the average daily gain of piglet, diarrhea rate and death and culling rate
Design
| Control group | Baby pig feedstuff+ |
| Test group 1 | 10% dosage of baby pig feedstuff-baby pig feedstuff (weight percent)+10% dosage of conventional microbiological fermented feed |
| Test group 2 | 10% dosage of baby pig feedstuff-baby pig feedstuff (weight percent)+10% dosage of functional microbial fermented feed |
The production of conventional microbiological fermented feed Shenyang Bo Shanyingsheng Bioisystech Co., Ltd, the name of an article are as follows: benefit times stick 801T
(piglet use)
The influence of table 13, energy property fermentative feedstuff of microbe to the average daily gain of piglet, diarrhea rate and death and culling rate
Note: experimental period is 30 days.
As shown in Table 13, the equivalent substitution addition functional microbial fermentation that 10% is carried out in the baby pig feedstuff of piglet is raised
Material, can be such that the average daily gain of piglet increases, and diarrhea rate reduces, and death and culling rate reduces, and compared with the control group, test group 1 is average
Daily gain increase by 6.82%, diarrhea rate rate of descent 22.72%, death and culling rate rate of descent 33.47%；2 average daily gain of test group increases
Add 9.09%, diarrhea rate rate of descent 40.13%, death and culling rate rate of descent 41.43%.As it can be seen that the functional fermentation of equivalent substitution addition
Feed intake can be improved in feed, improves food conversion ratio, and diarrhea is reduced, and reduces enteron aisle medication, and hair color is bright, can effectively improve son
The production performance of pig, achieves energy-saving and emission reduction purposes, and increases economic benefit.
Embodiment 7:
This patent product functionality fermentative feedstuff of microbe that embodiment 3 is obtained, growing and fattening pigs average daily gain and
The application test of the influence of feed-weight ratio.Firstly, carrying out preliminary experiment using fattening pig feed, preliminary experiment is 10 days, preliminary experiment
After, start formal test, carried out according to above-mentioned experimental design, and carries out the record statistics of experimental data
Table 14, functional microbial fermented feed are to the average daily gain of growing and fattening pigs and the influence experimental design of feed-weight ratio
| Control group | Fatten pig feed |
| Test group 1 | Fatten pig feed -10% dosage of fattening pig feed+10% dosage of conventional microbiological fermented feed |
| Test group 2 | Fatten pig feed -10% dosage of fattening pig feed+10% dosage of functional microbial fermented feed |
The production of conventional microbiological fermented feed Shenyang Bo Shanyingsheng Bioisystech Co., Ltd, the name of an article are as follows: benefit times stick 801T
(hog use)
Table 15, functional microbial fermented feed are to the average daily gain of growing and fattening pigs and the influence of feed-weight ratio
Note: experimental period is 30 days.
As shown in Table 15,10% equivalent substitution addition functional microbial fermentation is carried out in the fattening pig feed of growing and fattening pigs
Feed can be such that the average daily gain of growing and fattening pigs increases, and feed-weight ratio reduces, and compared with the control group, 1 average daily gain of test group increases
Rate 6.03%, feed-weight ratio rate of descent 5.22%；2 average daily gain increment rate 8.62% of test group, feed-weight ratio rate of descent
7.81%, equal otherness is significant.As it can be seen that equivalent substitution adds functional fermented feed, feed intake can be improved, improve food conversion
Rate reduces diarrhea, improves excrement state, and hair color is bright, whole to improve health degree, so that meat improves, can effectively improve and educates
The production performance and Slaughter of hog, achieve energy-saving and emission reduction purposes, and increase economical.
Claims (10)
1. a kind of production method of functional microbial fermented feed, it is characterised in that: dry with dregs of beans, fulvic acid powder, corn pulp
CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, radix scutellariae powder, astragalus membranaceus powder, Radix Isatidis powder in powder, wheat bran skin, bran powder and Chinese medicine are proferment
Material, using bacillus subtilis, bacillus licheniformis, Lactococcus lactis, beer yeast slurry as beneficial microbe colony；By withered grass bud
Spore bacillus, bacillus licheniformis, the seed liquor of three kinds of bacterium of Lactococcus lactis and beer yeast slurry mix in proportion with fermentation raw material,
By aerobic fermentation, anaerobic fermentation, it is passed through filtrated air fermentation and anaerobic fermentation four-stage, obtains functional microbial fermentation
Feed.
2. production method described in accordance with the claim 1, it is characterised in that: by weight, fermentation raw material composition are as follows: dregs of beans 10
~30 parts by weight, 5~15 parts by weight of fulvic acid powder, 10~30 parts by weight of Dried Corn Steep Liquor Powder, 10~30 parts by weight of wheat bran skin, rice bran
10~30 parts by weight of powder, 1~3 parts by weight of CORTEX PHELLODENDRI CHINENSE powder, 1~3 parts by weight of RHIZOMA COPTIDIS from Sichuan of China powder, 1~3 parts by weight of radix scutellariae powder, astragalus membranaceus powder 1
~3 parts by weight, 1~3 parts by weight of Radix Isatidis powder；Beneficial microbe colony weight ratio are as follows: bacillus subtilis seed liquor 30~40
Parts by weight, 30~40 parts by weight of bacillus licheniformis seed liquor, 20~30 parts by weight of Lactococcus lactis seed liquor, beer yeast slurry
20~30 parts by weight；The weight ratio of fermentation raw material and beneficial microbe colony is 100~120 parts by weight of fermentation raw material, beneficial to micro-
30~50 parts by weight of biological flora.
3. production method according to claim 1 or 2, it is characterised in that: viable count in bacillus subtilis seed liquor >=
In 10000000000 CFU/ml, bacillus licheniformis seed liquor viable count be >=100 hundred million CFU/ml, viable bacteria in Lactococcus lactis seed liquor
Number is >=80 hundred million CFU/ml, viable count is >=1,000 hundred million CFU/ml in beer yeast slurry.
4. production method according to claim 1 or 2, it is characterised in that: the quality of each substance in the fermentation raw material
Water content be with the beans dregs of rice≤10%, fulvic acid powder≤10%, corn paste dry powder≤10%, wheat bran skin≤10%, CORTEX PHELLODENDRI CHINENSE powder≤
10%, RHIZOMA COPTIDIS from Sichuan of China powder≤10%, Huang Qin powder≤10%, Radix Astragali powder≤10%, Radix Isatidis powder≤10%；The fermentation raw material it is total
Quality Han Shui Liang≤10%.
5. production method according to claim 1 or 2, it is characterised in that: by weight, fermentation raw material composition are as follows: beans
20 parts by weight of the dregs of rice, 10 parts by weight of fulvic acid powder, 20 parts by weight of Dried Corn Steep Liquor Powder, 20 parts by weight of wheat bran skin, 20 parts by weight of bran powder,
2 parts by weight of CORTEX PHELLODENDRI CHINENSE powder, 2 parts by weight of RHIZOMA COPTIDIS from Sichuan of China powder, yellow 2 parts by weight of celery powder, 2 parts by weight of astragalus membranaceus powder, 2 parts by weight of Radix Isatidis powder；
The weight ratio of beneficial microbe colony are as follows: 30 parts by weight of bacillus subtilis seed liquor, 30 weight of bacillus licheniformis seed liquor
Part, 20 parts by weight of Lactococcus lactis seed liquor, 20 parts by weight of beer yeast slurry；The weight of fermentation raw material and beneficial microbe colony
Than for 100 parts by weight of fermentation raw material, 50 parts by weight of beneficial microbe colony.
6. according to production method described in claim 1,2,3,4 or 5, it is characterised in that: specifically includes the following steps:
(1) preparation of fermentation raw material:
Fermentation raw material: dregs of beans, fulvic acid powder, Dried Corn Steep Liquor Powder, wheat bran skin, bran powder is crushed to 30~50 mesh respectively；Will in
CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, yellow celery powder, astragalus membranaceus powder, Radix Isatidis powder in medicinal material, are crushed to 60~100 mesh respectively；By required ratio
CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, yellow celery powder, astragalus membranaceus powder, Radix Isatidis powder in Chinese medicine is sufficiently mixed by example, will be in fermentation raw material
Dregs of beans, fulvic acid powder, Dried Corn Steep Liquor Powder, wheat bran skin are sufficiently mixed, then the two are sufficiently mixed spare；
(2) preparation of beneficial microbe colony:
Bacillus subtilis, bacillus licheniformis, Lactococcus lactis pass through single colonie separation, solid seed culture and liquid respectively
Seed culture；
By through overactivation bacillus subtilis, bacillus licheniformis, Lactococcus lactis slant strains carry out single colonie respectively
Separation, purifying, obtain bacillus subtilis, bacillus licheniformis, Lactococcus lactis single colonie seed；Carry out solid respectively again
Seed culture and liquid seeds culture obtain bacillus subtilis seed liquor, bacillus licheniformis seed liquor, Lactococcus lactis strain
Sub- liquid；In required ratio by bacillus subtilis seed liquor, bacillus licheniformis seed liquor, Lactococcus lactis seed liquor and beer
Yeast paste is sufficiently mixed spare；
(3) the solid fermentation stage:
Solid fermentation process is: by aerobic fermentation, anaerobic fermentation, it is passed through filtrated air fermentation and anaerobic fermentation four-stage,
Functional microbial fermented feed is obtained, specifically:
Fermentation raw material that first will be uniformly mixed adjusts pH value to 6.5~7.0 with sodium hydroxide, in required ratio by withered grass bud
Spore bacillus seed liquor, bacillus licheniformis seed liquor, fermented glutinous rice yeast bacterium seed liquor, Lactococcus lactis seed liquor and beer yeast slurry
Mixing is inoculated into prepared fermentation raw material, and fermenter or band with ventilation and breathing equipment are encased in after mixing well
Have in ventilation and breathing equipment fermentation bag, is compacted, covers tightly fermenter bung hole or tighten fermentation bag sack, be placed into solid fermentation vehicle
Between carry out solid fermentation；
1) aerobic solids fermentation stage: (the 1st day) 0~24 hour, standing for fermentation, room temperature are controlled at 28 DEG C~38 DEG C；
2) anaerobic solids fermentation stage: (the 2nd day) 24~48 hours, standing for fermentation, room temperature are controlled at 36 DEG C~38 DEG C；
3) it is passed through filtrated air fermentation stage: (the 3rd, 4 day) 48~96 hours, to the fermenter with air-breather or with logical
In device of air fermentation bag, filtrated air is injected by air pump, ventilatory capacity is 0.1~0.3V/V.min, and room temperature is controlled 28
DEG C~38 DEG C；
4) anaerobism (standing forms gemma and metabolite) fermentation stage: (5,6 days) 96~144 hours stop to ventilation
With the fermenter of breathing equipment or with air is passed through in ventilation and breathing equipment fermentation bag, room temperature is controlled 28 DEG C~38
℃；
(4) low temperature drying crushing stage:
After solid fermentation, fermentation material is transferred on negative-pressure fluidized-bed, carries out low temperature drying, inlet temperature control 80~
90 DEG C, outlet temperature is controlled at 40~50 DEG C, fermentation material 12~20min of residence time in fluidized bed, and being dried to water content is
10~16%, 70~90 mesh are crushed to using high speed pulverizing mill, obtain functional microbial fermented feed.
7. according to production method described in claim 1,2,3,4 or 5, it is characterised in that:
Its preferred steps are as follows:
(1) preparation of the raw material to ferment:
Fermentation raw material: dregs of beans, fulvic acid powder, Dried Corn Steep Liquor Powder, wheat bran skin, bran powder is crushed to 30 mesh respectively；By Chinese medicine
In CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China powder, yellow celery powder, astragalus membranaceus powder, Radix Isatidis powder, be crushed to 100 mesh respectively；In required ratio by Chinese medicine
CORTEX PHELLODENDRI CHINENSE powder, RHIZOMA COPTIDIS from Sichuan of China, yellow celery powder, astragalus membranaceus powder, Radix Isatidis powder in material are sufficiently mixed, by the dregs of beans in fermentation raw material, yellow corruption
Sour powder, Dried Corn Steep Liquor Powder, wheat bran skin are sufficiently mixed, then the two are sufficiently mixed spare；
(2) preparation of beneficial microbe colony:
Bacillus subtilis through overactivation, bacillus licheniformis, Lactococcus lactis slant strains are subjected to single colonie point respectively
From, purifying, obtain bacillus subtilis, bacillus licheniformis, Lactococcus lactis single colonie seed；Carry out solid kind respectively again
Son culture and liquid seeds culture, obtain bacillus subtilis seed liquor, bacillus licheniformis seed liquor, lactic acid goal coccus kind
Sub- liquid；In required ratio by bacillus subtilis seed liquor, bacillus licheniformis seed liquor, Lactococcus lactis seed liquor and beer
Yeast paste is sufficiently mixed spare；
(3) the solid fermentation stage:
Solid fermentation process is: by aerobic fermentation, anaerobic fermentation, it is passed through filtrated air fermentation and anaerobic fermentation four-stage,
Functional microbial fermented feed is obtained, specifically:
Fermentation raw material that first will be uniformly mixed adjusts pH value to 6.5 with sodium hydroxide, in required ratio by bacillus subtilis
Seed liquor, bacillus licheniformis seed liquor, fermented glutinous rice yeast bacterium seed liquor, Lactococcus lactis seed liquor and beer yeast slurry mixing,
It is inoculated into prepared fermentation raw material, the fermenter with ventilation and breathing equipment is encased in after mixing well or has ventilation
In breathing equipment fermentation bag, compacting covers tightly fermenter bung hole or tightens fermentation bag sack, is placed into the progress of solid fermentation workshop
Solid fermentation；
1) aerobic solids fermentation stage: (the 1st day) 0-24 hours, standing for fermentation, room temperature are controlled at 37 DEG C；
2) anaerobic solids fermentation stage: (the 2nd day) 24-48 hours, standing for fermentation, room temperature are controlled at 38 DEG C；
3) be passed through filtrated air fermentation stage: (the 3rd, 4 day) 48-96 hours is infused into fermenter or fermentation bag by air pump
Enter filtrated air, ventilation oxygen supply air quantity is 0.3V/V.min, and room temperature is controlled at 38 DEG C；
4) anaerobism (standing forms gemma and metabolite) fermentation stage (5,6 days) 96-144 hours, stop to ventilation and
The fermenter of breathing equipment or with air is passed through in ventilation and breathing equipment fermentation bag, room temperature is controlled at 38 DEG C；
(4) low temperature drying crushing stage:
After solid fermentation, fermentation material is transferred on negative-pressure fluidized-bed, carries out low temperature drying, inlet temperature is controlled 85
DEG C, outlet temperature is controlled at 45 DEG C, fermentation material residence time 16min in fluidized bed, and being dried to water content is 13%, using
High speed pulverizing mill is crushed to 80 mesh, obtains functional microbial fermented feed.
8. a kind of functional microbial fermented feed that any production method production of claim 1-7 obtains.
9. functional microbial fermented feed according to claim 8, it is characterised in that: product crude protein 30~33%,
Molten protein 10 .0~12.0% of acid, 11.0~15.0% lysine 3.1~3.3% of crude fibre, mannosan 3.0~7.0%,
VBN50~60mg/100g, ash content 9.0~11.0%, total acidity 2.1~2.3%, 1800~2000U/g of proteinase activity, suppression
Bacterium loop diameter is 10.0~12.0mm, ash content 13~18%, water content 11.0~13.0%；
It is preferred that product crude protein 33%, the molten protein 12 .0% of acid, crude fibre 15.0%, lysine 3.3%, mannosan
5.0%, VBN 60mg/100g, ash content 10.0%, total acidity 2.3%, proteinase activity 2000U/g, antibacterial circle diameter are
11mm, ash content 10%, water content 12.0%, and contain bacillus subtilis >=1,000,000,000 CFU/g, bacillus licheniformis >=1,000,000,000
CFU/g, Lactococcus lactis >=1.0 hundred million CFU/g, beer yeast live bacteria number are >=1.0 hundred million CFU/g.
10. application of functional microbial fermented feed during animal-breeding described in a kind of claim 8.
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Cited By (10)
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| CN110050902A (en) * | 2019-05-30 | 2019-07-26 | 南京宝辉生物饲料有限公司 | A kind of environmental emission reduction type laying hen biological fermentation feed and preparation method thereof |
| CN110050895A (en) * | 2019-05-30 | 2019-07-26 | 南京宝辉生物饲料有限公司 | A kind of environmental emission reduction type pig biological fermentation feed |
| CN110200160A (en) * | 2019-06-25 | 2019-09-06 | 江苏黑钻生物科技有限公司 | The herb fermenting feed and preparation method thereof for preventing African swine fever |
| CN110447780A (en) * | 2019-08-21 | 2019-11-15 | 广州博善生物科技股份有限公司 | A kind of functional feed preparation and application of the same food of sow sucking pig |
| CN110574840A (en) * | 2019-09-19 | 2019-12-17 | 河南亿万中元生物技术有限公司 | Traditional Chinese medicine fermented feed for preventing African swine fever and preparation method thereof |
| CN111213792A (en) * | 2020-02-17 | 2020-06-02 | 播恩生物技术股份有限公司 | Biological fermentation feed for improving piglet anemia and preparation method thereof |
| CN112617037A (en) * | 2021-01-07 | 2021-04-09 | 沈阳康缘益生水土环境修复研究所有限公司 | Preparation and application of feed active humic acid composite microbial agent |
| CN112877257A (en) * | 2021-03-18 | 2021-06-01 | 昆明理工大学 | Bacillus licheniformis and application thereof |
| CN113577161A (en) * | 2021-08-27 | 2021-11-02 | 河南牧翔动物药业有限公司 | Method for preparing antiviral Chinese herbal medicine preparation for livestock and poultry by sectional fermentation |
| NL2032906A (en) * | 2022-08-30 | 2022-09-28 | Univ Yunnan Agricultural | Fermentation method of chinese herbal medicine anti-stress feed additive for livestock and poultry |
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| CN110050902A (en) * | 2019-05-30 | 2019-07-26 | 南京宝辉生物饲料有限公司 | A kind of environmental emission reduction type laying hen biological fermentation feed and preparation method thereof |
| CN110050895A (en) * | 2019-05-30 | 2019-07-26 | 南京宝辉生物饲料有限公司 | A kind of environmental emission reduction type pig biological fermentation feed |
| CN110200160A (en) * | 2019-06-25 | 2019-09-06 | 江苏黑钻生物科技有限公司 | The herb fermenting feed and preparation method thereof for preventing African swine fever |
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| CN111213792B (en) * | 2020-02-17 | 2023-08-15 | 播恩生物技术股份有限公司 | Biological fermentation feed for improving piglet anemia and preparation method thereof |
| CN112617037A (en) * | 2021-01-07 | 2021-04-09 | 沈阳康缘益生水土环境修复研究所有限公司 | Preparation and application of feed active humic acid composite microbial agent |
| CN112877257A (en) * | 2021-03-18 | 2021-06-01 | 昆明理工大学 | Bacillus licheniformis and application thereof |
| CN113577161A (en) * | 2021-08-27 | 2021-11-02 | 河南牧翔动物药业有限公司 | Method for preparing antiviral Chinese herbal medicine preparation for livestock and poultry by sectional fermentation |
| NL2032906A (en) * | 2022-08-30 | 2022-09-28 | Univ Yunnan Agricultural | Fermentation method of chinese herbal medicine anti-stress feed additive for livestock and poultry |
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Application publication date: 20190412 |