CN112617037A - Preparation and application of feed active humic acid composite microbial agent - Google Patents
Preparation and application of feed active humic acid composite microbial agent Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Botany (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Inorganic Chemistry (AREA)
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- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a preparation method and application of a feed active humic acid composite microbial inoculum, which comprises the preparation of a feed active humic acid composite microbial inoculum fermentation culture medium, the selection of beneficial composite bacteria, the preparation process of the feed active humic acid composite microbial inoculum and the application of the feed active humic acid composite microbial inoculum to livestock. Wherein the raw materials of the fermentation medium comprise 20-35 parts of mineral humic acid, 10-30 parts of bean pulp, 25-35 parts of Chinese medicinal preparation, 20-35 parts of molasses and 2-6 parts of trace elements. The beneficial bacteria comprise 1-10 parts of bacillus subtilis, 1-10 parts of bacillus coagulans and 1-10 parts of saccharomyces cerevisiae. The active humic acid compound microbial agent can improve daily gain of the young animals in the lactation period, improve average daily gain of organisms after weaning, and reduce diarrhea rate, so that growth performance of the young animals after weaning is improved, and the active humic acid compound microbial agent has high economic value, effective safety and wide prospect.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to preparation and application of a feed active humic acid compound microbial agent.
Background
The livestock breeding industry in China is rapidly developed, but the problem of antibiotic and chemical drug residue is still one of important factors influencing the safety of animal-derived food, although the use of antibiotic in the animal growth process can prevent intestinal diseases, promote the growth of animals and improve the economic benefit of the breeding industry, the intestinal flora imbalance can be caused by excessive or illegal use of antibiotic, the intestinal ecological balance is damaged, the organism immunity is reduced, meanwhile, the drug residue of the breeding product can be caused, inevitable harm is caused to human and livestock and even the environment, the digestive tract of the animals after birth is not well developed and the function is incomplete, therefore, the animal cubs are difficult to rapidly adapt to new food structures due to the change of the food structures in the early weaning period, at the moment, the animal cubs in the early stage are severely weaned due to a plurality of factors such as food, environment and the like, the cubs are slowly developed and have low immunity, the existing biological fermentation feed has no obvious effects of promoting beneficial bacteria and antagonizing harmful bacteria on the digestive tract of the young animals at the early weaning stage, and has no obvious effects of enhancing the immunity and disease resistance of the organism, thereby increasing the diarrhea rate and death rate of the animals and bringing great economic loss to farms.
The microbial agent has the advantages of no toxic or side effect, no drug residue, no drug resistance and the like, can effectively supplement beneficial microorganisms in the digestive tract of animals, kill harmful microorganisms, improve the ecological balance of intestinal microorganisms, and improve the metabolic capability and the nutrient absorption capability of organisms, thereby promoting the growth of the organisms. The microbial agent has obvious effect because of the action mode of the microbial agent being the natural composition of the microorganism, and becomes an ideal antibiotic substitute. Beneficial compound bacteria are selected, a small-sized full-automatic fermentation tank is utilized, the traditional Chinese medicine micro powder and the strains are added into the fermentation tank for fermentation, the combined action of the Chinese herbal medicines and the active beneficial bacteria is utilized to prevent the intestinal diseases of animals, and the method has high economic value, and has wide prospects of effectiveness and safety.
Disclosure of Invention
In order to solve the problems of the prior art, the invention aims to provide a preparation method of a feeding active humic acid compound microbial agent and application of the feeding active humic acid compound microbial agent to animals. So as to obviously improve the intestinal microbial flora of animals, reduce diarrhea rate and death rate, and improve meat quality and egg yield.
In order to achieve the above purpose, the invention provides the following technical scheme:
1) weighing mineral source humic acid, bean pulp, a traditional Chinese medicine preparation, molasses and trace elements according to the required content of the raw materials of the feed active humic acid compound microbial agent fermentation medium.
2) And (3) adding the mixed powder of the fermentation medium into a fermentation tank by using process water until the mixed powder is pasty, putting the mixed powder into the fermentation tank, metering the volume, adjusting the pH value, sterilizing, cooling to room temperature, inoculating probiotics, fermenting at 25-35 ℃ for 2-6 days, stopping fermentation, filtering, and adjusting the pH value to obtain fermentation liquor, namely the feed active humic acid compound microbial agent.
Preferably, in the step 1), the addition ratio of the fermentation medium is as follows: 20-35 parts of mineral humic acid, 10-30 parts of bean pulp, 25-35 parts of a traditional Chinese medicine preparation, 20-35 parts of molasses and 2-6 parts of trace elements; the probiotic inoculation proportion in the step 2) is as follows: 1-10 parts of bacillus subtilis, 1-10 parts of bacillus coagulans and 1-10 parts of saccharomyces cerevisiae.
Preferably, the fermentation conditions in the step 2) further comprise that the pressure is 0-0.08MPa, the stirring speed is 100r/min-150r/min, the pH value of the liquid fermentation medium is adjusted to 4.0-7.0 before sterilization, and the pH value is adjusted to 1.5-3.5 after fermentation.
Preferably, the obtained active humic acid compound microbial agent is directly fed to the animal cubs for 2-20 mL/time/head, and 3 times a day.
The invention has the beneficial effects that:
beneficial compound bacteria are selected, a small-sized full-automatic fermentation tank is utilized, traditional Chinese medicine micro powder and strains are added into the fermentation tank for fermentation, the fermentation period is shortened, the number of effective viable bacteria is increased, the intestinal diseases of animals are prevented by utilizing the combined action of Chinese herbal medicines and active beneficial bacteria, and as the microbial inoculum has the advantages of no toxic or side effect, no medicine residue, no drug resistance and the like, the beneficial microorganisms in the digestive tract of the animals can be effectively supplemented, harmful microorganisms can be killed, the ecological balance of intestinal microorganisms is improved, the metabolic capability and the nutrient absorption capability of an organism are improved, so that the growth of the organism is promoted; the microbial agent has obvious effect because of the action mode of the natural components of the microorganisms, becomes an ideal antibiotic substitute, and has higher economic value and wide prospect of effectiveness and safety.
The humic acid of mineral source used in the invention is an organic macromolecular compound, is formed by decomposing and differentiating remains of animals and plants for a long time through microorganisms and slowly accumulating in a series of chemical reaction processes, is widely existed in peat, soil, lakes and other places, and has been used in the fields of feed additives and animal medical treatment for many years.
The Chinese medicinal preparation contains poplar flower extract, purslane, Chinese brake herb, plantain herb, weeping forsythia capsule, dandelion and coptis root which have the effects of clearing away heat and toxic materials, relieving swelling, eliminating stagnation, dispelling wind heat and the like.
The trace elements used in the invention contain iron, zinc and selenium, wherein the iron can promote the synthesis of hemoglobin and enhance the function of oxygen transportation in vivo; the zinc has very obvious effect on improving appetite, and the selenium has very obvious effect on resisting oxidation in vivo.
The probiotics used in the invention comprise bacillus subtilis, bacillus coagulans and saccharomyces cerevisiae. The bacillus is resistant to acid and high temperature, has high stability in an intestinal acid environment, can promote the growth of intestinal beneficial bacteria, antagonize harmful bacteria, enhance the immunity of organisms, improve the disease resistance, can metabolize to generate enzymes such as protease amylase and the like, can obviously improve the growth speed of animals, and promote the digestion of nutrient substances, and currently, more reported strains include beneficial strains such as bacillus subtilis, bacillus licheniformis, bacillus cereus, clostridium butyricum, bacillus coagulans and the like; the yeast cells are rich in protein, vitamins and various enzymes, can promote digestion and absorption of nutrients by animals, enhance the immunity of the animals, and can be metabolized to generate B vitamins, minerals, digestive enzymes, growth promoting factors and the like. Research shows that when the feed added with the compound microbial inoculum is used for feeding animals, the intestinal microbial flora of the animals is obviously improved, the diarrhea rate and the death rate are reduced, and the meat quality, the egg laying rate and the like are obviously improved.
The traditional Chinese medicine is used as a culture substrate, and the microbial fermentation traditional Chinese medicine has the advantages that the traditional Chinese medicine can metabolize or convert some components in the traditional Chinese medicine, the biological activity of the traditional Chinese medicine is changed, and meanwhile, the traditional Chinese medicine components can induce or inhibit microorganisms to generate new secondary metabolites. Traditional Chinese medicinal materials with homology of medicine and food and probiotics are fermented, and macromolecular substances of the traditional Chinese medicines are converted by microorganisms to finally become a novel microbial agent which can be quickly absorbed by intestinal tracts of people or animals.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for the purpose of illustration and description, and is in no way intended to limit the invention.
Example 1:
1) weighing 20 parts of mineral humic acid, 15 parts of bean pulp, 30 parts of a traditional Chinese medicine preparation, 20 parts of molasses and 4 parts of trace elements.
2) Adjusting the mixed powder of the fermentation medium to paste with water, adding into a fermentation tank, adjusting pH value after constant volume, sterilizing, and cooling to room temperature.
3) Inoculating probiotics, wherein the probiotics comprise 2 parts of bacillus subtilis, 1 part of bacillus coagulans and 3 parts of saccharomyces cerevisiae.
4) Fermenting for 5 days at 32 ℃ after inoculation, stopping fermentation, filtering the fermented substances through a 200-mesh sieve, and adjusting acid to finally obtain fermentation liquor, namely the feed active humic acid compound microbial agent.
5) The obtained fermentation liquor is directly fed to piglets, 5 mL/head once and 3 times a day.
Example 2:
1) weighing 20 parts of mineral humic acid, 20 parts of bean pulp, 30 parts of a traditional Chinese medicine preparation, 20 parts of molasses and 3 parts of trace elements.
2) Adjusting the mixed powder of the fermentation medium to paste with water, adding into a fermentation tank, adjusting pH value after constant volume, sterilizing, and cooling to room temperature.
3) Inoculating probiotics, wherein the probiotics comprise 3 parts of bacillus subtilis, 2 parts of bacillus coagulans and 3 parts of saccharomyces cerevisiae.
4) Fermenting for 4 days at 28 ℃ after inoculation, stopping fermentation, filtering the fermented substances through a 200-mesh sieve, and adjusting acid to finally obtain fermentation liquor, namely the feed active humic acid compound microbial agent.
5) The obtained fermentation liquor is directly fed to piglets, 5 mL/head once and 3 times a day.
Example 3:
1) weighing 20 parts of mineral humic acid, 30 parts of bean pulp, 30 parts of a traditional Chinese medicine preparation, 20 parts of molasses and 6 parts of trace elements.
2) Mixing the fermentation medium with the powder, adding water to obtain paste, adding into a fermentation tank, adjusting pH, sterilizing, and cooling to room temperature.
3) Inoculating probiotics, wherein the probiotics comprise 1 part of bacillus subtilis, 3 parts of bacillus coagulans and 3 parts of saccharomyces cerevisiae.
4) Fermenting for 4 days at 28 ℃ after inoculation, stopping fermentation, filtering the fermented substances through a 200-mesh sieve, and adjusting acid to finally obtain fermentation liquor, namely the feed active humic acid compound microbial agent.
5) The obtained fermentation liquor is directly fed to piglets, 5 mL/head once and 3 times a day.
Example 4:
200 piglets are selected and randomly divided into three groups according to litter size, wherein each group of piglets comprises 45, 54, 44 and 57 piglets, test group 1 is fed with the active humic acid compound microbial agent of example 1, test group 2 is fed with the active humic acid compound microbial agent of example 2, test group 3 is fed with the active humic acid compound microbial agent of example 3, a control group is not fed with the active humic acid compound microbial agent, the active humic acid compound microbial agent is fed from 3 days after the piglets are born to 5 days after weaning, the following data are obtained for 1 day, 3 times and 1 time, 5 mL/head:
according to the table, after the piglet is fed with the feed active humic acid composite microbial inoculum in the lactation period, the daily weight gain of the test group in the lactation period, the daily weight gain after weaning and the diarrhea rate are all remarkably superior to those of the control group. The invention not only can realize the health-care and epidemic-prevention functions of the active humic acid compound microbial agent on livestock, but also can improve the economic performance of the feed when in use.
The above description is only a preferred embodiment of the present invention, and any person skilled in the art may modify the present invention or modify it into an equivalent technical solution by using the technical solution described above. Therefore, any simple modifications or equivalent substitutions made in accordance with the technical solution of the present invention are within the scope of the claims of the present invention.
Claims (8)
1. The preparation and application of the feed active humic acid compound microbial agent are characterized in that a fermentation medium is prepared from the following raw materials: mineral humic acid, soybean meal, a traditional Chinese medicine preparation, molasses and trace elements.
2. The preparation method and the application of the feed active humic acid compound microbial preparation according to claim 1, wherein the main component of the traditional Chinese medicine preparation is a mixture of extracts of poplar flower, purslane, phoenix-tail fern, plantain herb, forsythia suspense, dandelion, coptis chinensis and the like.
3. The preparation method and the application of the feed active humic acid compound microbial agent according to claim 1, wherein the main components of the trace elements are iron, selenium and zinc.
4. The preparation method and the application of the feed active humic acid compound microbial agent according to any one of claims 1 to 3, wherein the inoculated probiotics are compound powder of bacillus subtilis, bacillus coagulans and saccharomyces cerevisiae.
5. The preparation method and the application of the feeding active humic acid compound microbial inoculum according to any one of the claims 1 to 4, wherein the preparation method of the feeding active humic acid compound microbial inoculum comprises the following steps:
1) weighing mineral source humic acid, bean pulp, a traditional Chinese medicine preparation, molasses and trace elements according to the required content of the raw materials of the feed active humic acid compound microbial agent fermentation medium.
2) Adjusting the mixed powder of the fermentation medium obtained in the step 1) to be pasty by using process water, putting the mixed powder into a fermentation tank, adjusting the pH value after constant volume, cooling to room temperature after sterilization, inoculating probiotics, fermenting for 2-6 days at 25-35 ℃, stopping fermentation, and adjusting the pH value after filtering to obtain fermentation liquor, namely the feed active humic acid compound microbial inoculum.
6. The preparation method of the feeding active humic acid composite microbial inoculum according to claim 5, wherein the addition proportion of the fermentation culture medium in the step 1) is 20-35 parts of mineral source humic acid, 10-30 parts of bean pulp, 25-35 parts of traditional Chinese medicine preparation, 20-35 parts of molasses and 2-6 parts of trace elements by weight; the probiotic inoculation proportion in the step 2) is 1-10 parts of bacillus subtilis, 1-10 parts of bacillus coagulans and 1-10 parts of saccharomyces cerevisiae by weight.
7. The preparation method of the feeding active humic acid composite microbial inoculum according to the claim 5, wherein the fermentation conditions of the step 2) further comprise the pressure of 0-0.08MPa, the stirring speed of 100r/min-150r/min, the pH value of the liquid fermentation medium before sterilization is adjusted to 4.0-7.0, and the pH value is adjusted to 1.5-3.5 when the fermentation is finished.
8. The preparation method and the application of the feed active humic acid compound microbial agent are characterized in that the obtained active humic acid compound microbial agent is directly fed to animal cubs for 2-20 mL/time/head and 3 times a day.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113180143A (en) * | 2021-05-24 | 2021-07-30 | 北京博思汇科生物技术有限公司 | Method for producing organic trace humic acid selenium of bacterial liquid additive |
| CN115463190A (en) * | 2022-08-22 | 2022-12-13 | 山农农牧(泰安)有限公司 | Fermented traditional Chinese medicine preparation for preventing and treating diarrhea of fattening sheep and preparation method thereof |
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| CN107494936A (en) * | 2017-08-01 | 2017-12-22 | 北京斯隆生物技术有限责任公司 | A kind of Chinese medicine humic acid feed for treating mastitis for milk cows and preparation method thereof |
| CN109601699A (en) * | 2018-12-28 | 2019-04-12 | 沈阳博善英胜生物技术有限公司 | A kind of functional microbial fermented feed and production method and application |
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| CN107494936A (en) * | 2017-08-01 | 2017-12-22 | 北京斯隆生物技术有限责任公司 | A kind of Chinese medicine humic acid feed for treating mastitis for milk cows and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113180143A (en) * | 2021-05-24 | 2021-07-30 | 北京博思汇科生物技术有限公司 | Method for producing organic trace humic acid selenium of bacterial liquid additive |
| CN115463190A (en) * | 2022-08-22 | 2022-12-13 | 山农农牧(泰安)有限公司 | Fermented traditional Chinese medicine preparation for preventing and treating diarrhea of fattening sheep and preparation method thereof |
| CN115463190B (en) * | 2022-08-22 | 2023-11-10 | 山农农牧(泰安)有限公司 | Fermented traditional Chinese medicine preparation for preventing and treating diarrhea of fattening sheep and preparation method |
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