KR102492755B1 - Method for preparing fermented total mixed ration using microbial strain complex and steam treatment - Google Patents

Abstract

The present invention supplies high-temperature steam to the raw material at once to constantly adjust the moisture content of the raw material to increase fermentation efficiency, which is excellent in digestibility and high palatability. it's about
The method for producing a completely blended fermented feed of the present invention includes the steps of (a) preparing a fermented strain including a B. subtilis strain and an L. acidophilus strain; (b) preparing a mixed fermented feed by mixing the fermented strain with feed for culture; (c) preparing a raw material mixture by mixing the raw materials of the complete feed; (d) supplying steam to the raw material mixture to prepare a steam treated raw material mixture; (e) preparing a fully formulated feed by mixing the steam treated raw material mixture, the mixed fermented feed, the EM probiotic containing S. cerevisiae and additives; and (f) preparing a complete fermented feed by packaging the complete feed and then performing secondary fermentation.

Description

Method for preparing fermented total mixed ration using microbial strain complex and steam treatment}

The present invention controls the fermentation rate and digestibility through steam treatment, and ferments raw materials using a microbial complex strain to have a beneficial effect on the health and growth of livestock, and a completely mixed fermented feed with excellent palatability (fermented total mixed ration, It relates to a method for preparing fermented TMR).

Recently, demand for high-quality livestock products with excellent meat quality and taste is increasing due to the improvement of national income and cultural level. In order to produce livestock products, various studies are being conducted to improve the quality of feed supplied to livestock products.

In particular, ruminants such as cows are herbivores including beef cattle for meat production and dairy cattle for milk production, and use grass-like plants as their main energy source. Hay feed such as corn, barley, beet pulp, cottonseed, etc. is also mixed and supplied as an energy source. there is.

Completely formulated feed is prepared by mixing forage and enriched feed at an appropriate ratio in consideration of the nutritional and physiological characteristics of ruminants. , Due to this, when livestock with rumen, such as cows and sheep, consume feed, the ingested feed stays in the stomach for a long time, so that it is appropriately stirred by gastric motion and fermented by the action of various bacteria or enzymes in the stomach to sufficiently absorb nutrients. It is a feed that has been formulated to do so.

Therefore, the complete compound feed can prevent vegetarian competition between individuals through complete mixing of forage and compound feed, as well as prevent metabolic diseases through the balance of nutrients, stabilize fermentation in the rumen, improve feed intake, and increase the amount of nutrients. It plays an important role in obtaining the effect of increasing livestock productivity, such as increased utilization efficiency and daily weight gain.

On the other hand, ruminants have a stomach (stomach) of a huge and complex structure, unlike unit animals such as pigs and chickens. The stomach of ruminants can be divided into four major compartments. The first and second stomachs are collectively called the rumen, and various microorganisms inhabit the rumen. Microorganisms in the rumen ferment feed crops such as grass and hay consumed by ruminants to produce short carbon chain fatty acids, and the produced fatty acids are absorbed through the rumen wall and used as energy sources for ruminants. 70% of the total energy used for maintenance, growth, and production of livestock products (meat and milk) is volatile fatty acids generated in the rumen, and the production and composition of volatile fatty acids produced in the rumen directly affect the productivity of beef cattle and milk. going crazy

In the past, in order to improve the nutrient utilization efficiency of complete feed containing a large amount of fiber, various enzymes produced by microorganisms were used to change the physicochemical properties of feed ingredients and introduce microorganisms into complete feed to increase nutrient utilization efficiency. Fermented feed is produced and supplied, and fermented water prepared by mixing hot water heated to 100 ℃ with cooling water to maintain the fermentation temperature of 35 ℃ is supplied to the feed and fermented. When using fermented feed, it is difficult to accurately control the moisture content of the fermented feed due to its own moisture content in the raw material, and there is a problem that the moisture content of the fermented feed exceeds 50% and the fermentation efficiency decreases. Do.

In addition, fermented feeds with perfect mix are fermented by the growth and metabolic process of microorganisms introduced for fermentation, and the fermentation characteristics and efficiency depend on the type and type of substrate used by microorganisms, temperature, oxygen and water activity, etc. Since it appears differently depending on the same environmental factors and the type of microorganisms involved in fermentation, it is very important to select an appropriate microbial strain in order to prepare a perfectly formulated fermented feed for a desired purpose.

However, in the past, heterogeneous strains were mixed and fermented, and through steam pre-treatment of the raw materials, a fully-mixed fermented feed with excellent nutritional composition and functionalities such as improved digestibility of breeding livestock, improved growth and activity, and increased production was produced. Research on the related technology for the manufacturing method is insufficient, so research on this is necessary.

Korean Patent Registration No. 10-1795499 (Announcement date: 2017.11.10) Korean Patent Registration No. 10-1883626 (Announcement date: 2018.07.30) Korean Patent Registration No. 10-1476107 (Announcement date: 2014.12.24) Korean Patent Registration No. 10-1641996 (Announcement date: 2016.07.25)

The present invention has been made to solve the problems of the prior art as described above, by supplying high-temperature steam to the raw material at once to constantly adjust the moisture content of the raw material to increase the fermentation efficiency and improve the digestibility of perfectly mixed fermented feed. It is intended to provide technical information about the manufacturing method.

In addition, the present invention promotes the health and growth of livestock using a complex fermentation strain including a Bacillus sp. strain, a Lactobacillus sp. strain, and a Saccharomyces sp. strain. It is intended to provide technical information on a method for producing a completely blended fermented feed that has a beneficial effect on and has excellent palatability.

In addition, the present invention is to provide technical details related to a method for producing a perfectly formulated fermented feed having a suitable component ratio for each growth stage of livestock.

The technical problems to be solved by the present invention are not limited to the above-mentioned technical problems, and other technical problems not mentioned are clearly understood by those skilled in the art from the description below. It could be.

In order to achieve the technical problem as described above, the present invention, (a) Bacillus subtilis obtained from cow feces ( Bacillus subtilis ) strain and Lactobacillus acidophilus ( Lactobacillus acidophilus ) To prepare a fermentation strain containing the strain doing; (b) mixing the fermentation strain with feed for culture to prepare a pre-fermentation mixed feed, and first fermenting the pre-fermentation mixed feed to prepare a mixed fermented feed; (c) preparing a raw material mixture by mixing the raw materials of the complete feed; (d) preparing a steam-treated raw material mixture by supplying steam at 80 to 130 °C to the raw material mixture for 1 to 120 minutes; (e) mixing the steam-treated raw material mixture, the mixed fermented feed, an EM probiotic including Saccharomyces cerevisiae and an additive to prepare a complete feed; and (f) packaging the completely mixed feed and subjecting it to secondary fermentation to produce a perfectly mixed fermented feed.

In addition, the raw material mixture is annual grass, protein skin, corn flakes, main vein, soybean oil meal, alfalfa bale, tall fescue, timothy, yeonmak, cotton vein, corn germ meal, DDGS, wheat hull, almond meal, water, lupine, and beet pulp It may include one or more selected from the group consisting of.

According to a preferred embodiment of the present invention, the feed for culture may be prepared by supplying steam at 80 to 130 ° C. and treating the raw material for feed for culture with steam for 1 to 120 minutes.

According to a preferred embodiment of the present invention, in the step (b), the pre-fermentation mixed feed is prepared by mixing the fermentation strain at a concentration of 1 × 10 ⁴ to 1 × 10 ⁹ CFU / g, 30 to 60 A mixed fermented feed may be prepared by fermenting the primary fermentation for 3 to 10 days under conditions in which the temperature of ° C. is maintained.

According to a preferred embodiment of the present invention, the additives are salt, seasoning, sugars, flavoring agents, volatile fatty acids, molasses fermentation concentrates (Condensed Molasses Fermentation Solubles, CMS), L-ascorbic acid, choline chloride, inositol, vitamins, Potassium chloride, iron citrate, magnesium oxide, amino acid, organic acid or its salt, antioxidant, antifungal agent, emulsifier, colorant, potassium chloride, limestone, lincal agent, sodium bicarbonate, zinc agent, yeast, antioxidant, bypass fat and bypass protein It may include one or more selected from the group consisting of.

According to a preferred embodiment of the present invention, the complete feed may have a moisture content of 30 to 45%.

According to a preferred embodiment of the present invention, in step (f), the packaged complete feed is fermented at 35 to 45 ° C. for 2 to 10 days, and then secondary fermentation at 25 to 35 ° C. for 3 to 10 days. It is possible to prepare a completely mixed fermented feed.

According to a preferred embodiment of the present invention, the completely mixed fermented feed is used as a cow feed and dairy cow feed at one or more growth stages selected from the group consisting of a growing period, an early fattening period, a middle fattening period, a late fattening period, a dry period, and a breeding period. can

According to the method for manufacturing a perfectly mixed fermented feed according to the present invention, it is possible to effectively control the moisture content of raw materials by supplying high-temperature steam at once, thereby improving the fermentation efficiency of the feed and achieving economic feasibility by lowering the feed manufacturing cost. there is.

In addition, it is possible to easily control the fermentation rate of raw materials, block the proliferation of harmful microorganisms, increase the rate of hydrolysis of raw materials by microbial fermentation, improve digestibility, and produce wet-type, fully-mixed fermented feed with excellent palatability. can be manufactured

In addition, according to the method for producing a completely mixed fermented feed according to the present invention, using a complex fermentation strain including a strain of the genus Bacillus, a strain of the genus Lactobacillus and a strain of the genus Saccharomyces has a beneficial effect on the health and growth of livestock The strain can produce a fully formulated fermented feed.

In addition, according to the method for producing a fully-mixed fermented feed according to the present invention, a customized fully-mixed fermented feed having an appropriate component ratio for each growth stage of livestock can be manufactured, thereby contributing to the development of the livestock industry.

1 is a process chart showing a method for producing a completely mixed fermented feed according to the present invention.

Hereinafter, with reference to the accompanying drawings, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. However, since the description of the present invention is only an embodiment for structural or functional description, the scope of the present invention should not be construed as being limited by the embodiments described in the text. That is, since the embodiment can be changed in various ways and can have various forms, it should be understood that the scope of the present invention includes equivalents capable of realizing the technical idea. In addition, since the object or effect presented in the present invention does not mean that a specific embodiment should include all of them or only such effects, the scope of the present invention should not be construed as being limited thereto.

The meaning of terms described in the present invention should be understood as follows.

Terms such as "first" and "second" are used to distinguish one component from another, and the scope of rights should not be limited by these terms. For example, a first element may be termed a second element, and similarly, a second element may be termed a first element. It should be understood that when an element is referred to as “connected” to another element, it may be directly connected to the other element, but other elements may exist in the middle. On the other hand, when an element is referred to as being “directly connected” to another element, it should be understood that no intervening elements exist. Meanwhile, other expressions describing the relationship between components, such as “between” and “immediately between” or “adjacent to” and “directly adjacent to” should be interpreted similarly.

Singular expressions should be understood to include plural expressions unless the context clearly dictates otherwise, and terms such as “comprise” or “having” refer to a described feature, number, step, operation, component, part, or It should be understood that it is intended to indicate that a combination exists, and does not preclude the possibility of the presence or addition of one or more other features, numbers, steps, operations, components, parts, or combinations thereof.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs, unless defined otherwise. Terms defined in commonly used dictionaries should be interpreted as consistent with meanings in the context of related art, and cannot be interpreted as having ideal or excessively formal meanings unless explicitly defined in the present invention.

Hereinafter, the present invention will be described in detail.

1 is a process chart showing a method for producing a completely mixed fermented feed according to the present invention.

Referring to Figure 1, the method for producing a completely blended fermented feed according to the present invention, (a) preparing a fermentation strain; (b) preparing a mixed fermented feed by mixing the fermented strain with feed for culture; (c) preparing a raw material mixture by mixing the raw materials of the complete feed; (d) supplying steam at 80 to 130° C. to the raw material mixture for 1 to 120 minutes to prepare a steam treated raw material mixture; (e) mixing the steam treated raw material mixture, the mixed fermented feed, EM probiotic and water to prepare a complete feed; and (f) preparing a complete fermented feed by packaging the complete feed and then performing secondary fermentation.

The step (a) is a step of preparing a fermentation strain for producing a completely mixed fermented feed.

Specifically, the fermented strain solves the problem of non-uniform content and distribution of components in the complete feed, thereby producing a completely blended feed having a uniform content of nutrients, and converting non-digestible substances into digestible substances through fermentation treatment. can produce fermented feed with excellent digestibility, supply proteins, vitamins, antibiotics, and growth factors produced by fermented strains, as well as enhance the palatability and safety of completely blended feeds, resulting in high-quality, high-quality complete blended fermentation feed can be produced.

When the fully-mixed fermented feed prepared by the manufacturing method according to the present invention is fed to ruminants, in particular, beef cattle, various nutrients are directly absorbed into the blood and can be easily used as an energy source. By producing a large amount of volatile fatty acids, etc., livestock fattening and weight gain are smoothly performed, and by increasing the content of unsaturated fatty acids in the body, it is possible to suppress abnormal fermentation due to the feeding of a large amount of concentrated feed, and non-useful for amino acids It is possible to improve the nutrient utilization efficiency by relatively reducing the action of microorganisms.

In particular, the fully mixed fermented feed prepared by the manufacturing method according to the present invention can improve the weight gain by increasing the feed intake during the feeding period and improve the meat quantity grade by increasing the loin cross-sectional area.

As the fermentation strain, a mixed strain including a Bacillus subtilis sp. strain and a Lactobacillus acidophilus sp. strain may be used as the fermentation strain.

Specifically, Bacillus subtilis strains do not accumulate in the body, do not cause resistance to pathogenic microorganisms, and improve productivity of livestock. In addition, it inhibits the proliferation or establishment of harmful bacteria proliferating in the intestine, directly helps normalize the body or normalize the intestinal flora, and metabolites detoxify harmful substances such as cell toxins produced by harmful bacteria in the intestine, or suppress the production can hinder

The Bacillus subtilis strain is a spore-forming bacterium that is stable to heat and pH, can survive for a long time in the intestines of livestock, and can survive for a long time in commercial products without being frozen. In addition, it is possible to suppress the generation of pathogens, generate various enzymes such as cellulase, amylase, protease, etc. to decompose various raw materials included in the raw material mixture to improve the digestibility of livestock fed feed.

The Lactobacillus acidophilus strain is a lactic acid bacillus that undergoes homozygous or heterozygous fermentation, and is commonly found in the intestinal tract of animals and in the fermentation process of dairy products and vegetables. ) or Clostridium ( Clostridium ), as well as inhibiting the propagation of harmful bacteria and improving diarrhea and constipation, as well as vitamin synthesis, anti-cancer activity, and lowering serum cholesterol.

In addition, it improves palatability and digestibility to improve feed conversion rate, reduces stress, diarrhea, enteritis, etc., strengthens resistance to diseases, suppresses abnormal fermentation in the intestines, increases milk fat and flow rate, and causes metabolic diseases can reduce

In addition, the Lactobacillus acidophilus strain produces various organic acids such as acetic acid, propionic acid, butyric acid, etc. during the fermentation process, thereby weakening the structural rigidity of the fibers contained in the complete fiber feed, thereby improving the digestibility in the rumen. , can promote the production of essential amino acids such as lysine and arginine.

The mixed strain as described above can proliferate in a short time in the feed together with the Saccharomyces servizia strain introduced into the EM probiotic to be described later during fermentation of the complete feed.

Specifically, when a complete feed is fermented using Bacillus subtilis, Lactobacillus acidophilus, and Saccharomyces serviae at the same time, the fermentation time is shortened to prevent an increase in the manufacturing cost of the fermented feed. In the case of Lactobacillus acidophilus and Saccharomyces servizia, the cell growth time is long, so contaminants easily proliferate in a short time during fermentation, but when fermented with Bacillus subtilis, such can solve the problem. The Bacillus subtilis strain can also promote protein degradation by producing proteases, and can effectively decompose insoluble substances contained in completely formulated feeds to improve digestion and absorption rate.

As described above, the mixed strain containing Bacillus subtilis and Lactobacillus acidophilus can be obtained from cow feces, and the mixed strain is collected from cow feces, then serially diluted and placed in a special medium. A strain that grows by inoculation can be obtained and used.

In this way, the reason for using the Bacillus subtilis strain and the Lactobacillus acidophilus strain collected from the same sample, cow feces, is that the strain is already co-cultured in the stomach of a cow at the same time and has high affinity, so it is a perfectly formulated feed. This is because the fermentation time can be greatly shortened.

Therefore, Bacillus subtilis and Lactobacillus acidophilus obtained through the above methods normally survive in the intestines of livestock, can survive even when passing through the digestive tract, and can proliferate or settle in the intestines. In other words, it produces a large amount of digestive enzymes to select those that have a beneficial effect on the host.

Bacillus subtilis and Lactobacillus acidophilus strains can be obtained by inoculating a cow feces sample into a medium, respectively, and specifically, 2% by weight of glucose, 2% by weight of corn starch, 5% by weight of cellulose, 5% by weight of hemicellulose %, 10% by weight of pectin, 2% by weight of protein skin powder, 0.5% by weight of peptone, 0.5% by weight of yeast extract, 0.2% by weight of calcium carbonate (CaCO ₃ ), 0.01% by weight of antifoaming agent, 22.7% by weight of agar and the rest A heterogeneous strain that produces large transparent colonies in the same culture medium by inoculating a cow feces sample collected in a culture medium containing water at pH 4 and culturing for 24 to 120 hours under aerobic conditions at a temperature of 30 to 50 ° C. can be obtained and used.

In addition, it is preferable to use a fecal sample obtained from Korean cattle classified as first grade or higher as a fecal sample used to obtain the Bacillus subtilis strain and the Lactobacillus acidophilus strain.

The Bacillus subtilis strains and Lactobacillus acidophilus strains obtained through this method exhibit excellent proliferation rates even in an acidic environment, are capable of anaerobic fermentation under the same conditions as cow stomachs, and are difficult to decompose polymers such as cellulose and pectin. It is possible to prepare a fully-mixed fermented feed having excellent nutritional composition due to excellent decomposition efficiency of components and functionalities such as improving digestibility of breeding livestock, improving growth and activity, and increasing production. In particular, the Bacillus subtilis strain and the Lactobacillus acidophilus strain have excellent survival rates even when the moisture content is 30 to 45%, preferably, 35 to 40%, and thus completely It can be utilized for manufacturing compounded fermented feed.

In the step (b), as a step of preparing a mixed fermented feed using the fermentation strain, the feed for culture is mixed with the fermented strain to prepare a pre-fermentation mixed feed, and the pre-fermentation mixed feed is first fermented to obtain a mixed fermented feed can be manufactured

The feed for culture can be prepared by introducing raw materials including hay, bark, meal, and additives of the same kind as the raw material mixture to be described later. It can be configured to prevent proliferation.

Preferably, the feed for culture may use a steam treatment by heating the raw material of the feed for culture using steam at 80 to 130 ° C. for 1 to 120 minutes, and the moisture content is 30 to 45% through steam treatment. Mixed feed for culture can be used. The feed for culture treated with steam through the above method not only kills contaminant bacteria, but also increases the reactivity of indigestible polymers such as cellulose, hemicellulose, and pectin contained in the raw material for culture feed, resulting in hydrolysis by fermenting strains. Not only can the growth of the fermentation strain be greatly increased by greatly improving the efficiency, but also the production of nutrients can be promoted.

In addition, in this step, in order to prepare a mixed fermented feed, the mixing of the fermented strain and the feed for pretreatment culture is performed by mixing the Bacillus sp. strain and the Lactobacillus sp. strain to a concentration of 1 × 10 ⁴ to 1 × 10 ⁹ CFU/g, respectively. It can be mixed to prepare a pre-fermentation mixed feed.

The pre-fermentation mixed feed prepared by the above method can be fermented for primary fermentation for 1 to 10 days under conditions of maintaining a temperature of 30 to 60 ° C. to prepare a mixed fermented feed. The fermented feed can be used for preparing a completely blended fermented feed by fermentation after being added during the production of a complete blended feed, which will be described later.

Preferably, feed for cultivation may include annual ryegrass, whole cotton, protein skin, corn flakes, main veins and long oil cakes, and may have a moisture content of 30 to 45%, and a moisture content of 30% When it exceeds , it is difficult for the fermentation strain to proliferate uniformly, and when it exceeds 45%, there is a problem in that the growth of the fermentation strain does not significantly increase. Preferably, the feed for culture may be adjusted so that the moisture content is 40%.

In the step (c), the raw material mixture is prepared by mixing the raw materials of the completely mixed feed.

The raw material of the complete feed may include, but is not limited to, hays, barks, meal, additives, etc. commonly used for the manufacture of TMR feeds.

For example, hays can introduce fiber-rich materials, such as alfalfa, sudan grass, orchard grass, italian ryegrass, red clover, ladino clover, straw, annual ryegrass, deerifcus, corn hay, barley hay, sugar cane hay, sorghum, wheat, softbark, lupin or mixtures thereof can include

Bark is a grain by-product that has a high content of crude fiber, etc., so it can be added when manufacturing fully mixed fermented feed. It may include blood, lupine skin, jade skin, rice bran, skim steel, or a mixture thereof.

For meal, a by-product obtained by extracting the liquid fraction such as oil or moisture and powdering the remaining part is used. Gourd (corn germ meal, corn oil meal), rapeseed meal, sunflower meal, distillers dried grains with solubles (DDGS), citrus peel, apple peel, brewer's meal, whole cotton, beet pulp, rice with rice, okara , pulse root, or mixtures thereof.

Preferably, annual grass, protein hulls, corn flakes, ginseng veins, jang oil flakes, alfalfa bale, tall fescue, timothy, soft veins, cotton veins, corn germ meal, DDGS (dried distiller's grains with solubles, = liqueur), wheat hulls, A raw material mixture comprising almond meal, lupine, beet-pulp, dereifcus or a mixture thereof may be prepared.

In addition, the raw material mixture as described above may be prepared by introducing cut raw materials cut to a uniform size, but is not limited thereto.

In the step (d), a vapor treatment raw material mixture is prepared by supplying steam at 80 to 130 ° C. to the raw material mixture for 1 to 120 minutes.

In this step, the steam treatment raw material mixture may be prepared by supplying the raw material mixture to a chamber supplied with steam or a vessel supplied with steam, and supplying steam at 80 to 130 ° C. for 1 to 120 minutes.

Steam treatment as described above not only kills contaminant bacteria remaining in the raw material mixture, but also greatly improves the hydrolysis efficiency by fermentation strains by increasing the reactivity of indigestible polymers such as cellulose, hemicellulose, and pectin contained in the raw material mixture. Not only can the growth of the fermentation strain be greatly increased, but also the production of nutrients can be promoted.

In addition, compared to using fermented water prepared by mixing hot water and cooling water through steam treatment, the water content of the raw material mixture can be easily controlled, thereby controlling the fermentation rate.

Preferably, in this step, the raw material mixture is supplied to a chamber where steam is supplied, and steam at 80 to 100 ° C. is supplied for 20 to 60 minutes to prepare a steam treated raw material mixture having a moisture content of 30 to 45%, The prepared steam treatment raw material mixture may be stored at a temperature of 25 ° C to 30 ° C, and more preferably, a steam treatment raw material mixture having a moisture content of 40% may be prepared.

When a steam-treated raw material mixture having a water content of 30 to 45% is prepared as described above and stored at a temperature of 25 ° C or higher, the complete feed is self-fermented at 40 ° C or higher by fermentation with EM probiotics, which will be described later, regardless of the season. can be produced, thereby greatly shortening the fermentation time.

In addition, it can block the growth of harmful microorganisms, control the fermentation rate and digestibility of completely mixed feed, and ferment raw materials using microbial complex strains to have a beneficial effect on the health and growth of livestock. Wet type with excellent palatability of fermented total mixed ration (fermented TMR) can be prepared.

On the other hand, in the step (e), the raw material mixture, mixed fermented feed, Saccharomyces cerevisiae strain ( Saccharomyces cerevisiae sp.) is a step of mixing the EM probiotic and additives to prepare a complete feed.

In this step, the raw material mixture, mixed fermented feed, EM probiotic and water are mixed in a mixing tank, and then mixed uniformly to prepare a completely formulated feed.

The EM probiotic used to manufacture the complete feed mix creates the substrate necessary for the growth of the fermented strain inoculated into the mixed fermented feed, and the livestock ingesting the complete feed feed contain ammonia (NH ₃ ) and hydrogen sulfide (H ₂ ). The emission of harmful gases such as S) is reduced, which not only improves the productivity of livestock, but also improves the environment in the barn, and suppresses the occurrence of decreased appetite and nervousness.

In particular, the EM probiotic may introduce a strain of the genus Saccharomyces, preferably Saccharomyces servizier, and a probiotic sample at a concentration of 1 × 10 ⁷ to 1 × 10 ⁹ CFU / g can be used

Saccharomyces servizieri produces vitamin B complex, selenium, and essential amino acids to strengthen the liver function of fed livestock, promotes intestinal function by producing digestive enzymes, and produces immune enhancing factors such as beta-glucan to improve livestock health. can improve your health.

In addition, β-glucan, a high-molecular polysaccharide extracted from the cell wall of Saccharomyces servigee, has not only immune enhancement and anticancer effects, but also pharmacological effects on blood cholesterol lowering, antibacterial, antiviral, and anti-inflammatory diseases. , can improve intestinal microflora and immune function.

In addition, in this step, if necessary, water is additionally mixed to prepare a completely mixed feed, and water gives moisture to the completely mixed feed to promote the growth of fermented strains and strains included in EM probiotics, raw material mixture to promote fermentation that decomposes the nutrients contained in

In addition, the additives added to the complete feed are salt, seasoning, sugars, flavors, volatile fatty acids, molasses fermentation concentrates (Condensed Molasses Fermentation Solubles, CMS), L-ascorbic acid, choline chloride, vitamins including inositol (usually (including minerals), minerals such as potassium chloride, iron citrate, magnesium oxide, and phosphates, amino acids such as lysine, alanine, and methionine, organic acids or salts thereof such as fumaric acid, butyric acid, and lactic acid, and antioxidants such as vitamin C and vitamin E. , antifungal agents such as calcium propionate, emulsifiers such as lecithin and glycerin fatty acid esters, pigments, potassium chloride, limestone, lyncal agents, sodium bicarbonate, magnesium oxide, salt, zinc agents, yeast, antioxidants, bypass fats, bypass proteins, or Mixtures of these may be included.

In addition, the additive may further include a supplement, and the supplement may be used in combination with a vitamin mix, a mineral mix, etc., and a commercially available milking TMR mix or fattening TMR mix is a representative example, and the use of a fully formulated fermented feed You can optionally formulate a vitamin supplement that suits your purpose.

Specifically, the milking TMR mix contains vitamin A, vitamin D3, vitamin E, iron sulfate, magnesium oxide, manganese sulfate, calcium phosphate, calcium carbonate, copper sulfate, cobalt sulfate, zinc sulfate, calcium iodate, potassium chloride and sodium chloride. As a mixture, productivity can be maximized by increasing milk fat and milk protein content and promoting digestion and absorption of nutrients and energy in feed.

In addition, the fattening TMR mix contains vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B9, methionine, lysine hydrochloride, calcium pantothenate, nicotinic acid, choline chloride, manganese sulfate, zinc sulfate, chloride As a mixture containing cobalt, copper sulfate, sodium acelanate, and iron sulfate, supplementation of vitamin A, which tends to be deficient in beef cattle feed, can be expected to promote growth, increase body weight, and improve meat quality.

Preferably, the additives may include salt, limestone, sodium bicarbonate, fermented feed, CMS, supplements (milk TMR mix, fattening TMR mix) or mixtures thereof.

In addition, the completely mixed fermented feed according to the present invention can adjust the composition of the completely mixed horse feed so that fermented feed having different component contents can be fed according to the growth stage of breeding livestock.

In terms of cows, cows have growth stages such as young calf, fattening period, early fattening period and late fattening period, dry period, breeding period, and female slaughter period. In order to do so, the composition of the raw material mixture may be adjusted.

According to a preferred embodiment of the present invention, for young calves, 6 parts by weight of alfalfa bale, 11 parts by weight of annual ryegrass, 2 parts by weight of timothy, 4 parts by weight of synthetic wax, 3 parts by weight of whole cotton, 4 parts by weight of natural wax, A raw material mixture containing 20.5 parts by weight of protein skin, 3 parts by weight of corn flakes, 20 parts by weight of main vein, 2 parts by weight of almond meal, 3 parts by weight of beet pulp, and 7.5 parts by weight of soybean oil cake was prepared, and the prepared raw material mixture was steam-treated. Then, 0.5 parts by weight of EM probiotics, 12 parts by weight of mixed fermented feed, 0.2 parts by weight of salt, 0.3 parts by weight of sodium bicarbonate, 0.5 parts by weight of limestone, 0.5 parts by weight of fattening TMR mix, and a completely mixed feed having a moisture content of 40 is prepared. Then, it can be fermented in step (f) to be described later to prepare a complete fermented feed, and 8 to 14 kg of the prepared fermented feed can be fed per day.

According to a preferred embodiment of the present invention, in the fattening and growing period, 6 parts by weight of alfalfa bale, 9 parts by weight of annual ryegrass, 6 parts by weight of tall fescue, 2 parts by weight of timothy, 2 parts by weight of synthetic soft wheat, 2.2 parts by weight of whole cottonseed, natural A raw material mixture containing 4 parts by weight of smoked corn, 14.20 parts by weight of protein skin, 7.9 parts by weight of corn flakes, 6 parts by weight of corn meal, 19 parts by weight of main vein, 2 parts by weight of lupine, and 6 parts by weight of jang oil cake was prepared and prepared. After steaming the raw material mixture, 0.5 parts by weight of EM probiotic, 10 parts by weight of mixed fermented feed, 2 parts by weight of CMS, 0.2 parts by weight of salt, 0.5 parts by weight of limestone, 0.5 parts by weight of fattening TMR mix, containing 40% moisture content After preparing the complete feed, it can be fermented in step (f) to produce a complete feed, and 8 to 14 kg of the prepared feed can be fed per day.

In addition, in the first half of fattening, 9 parts by weight of annual ryegrass, 6 parts by weight of tall fescue, 9 parts by weight of whole cotton, 2 parts by weight of natural wax, 6 parts by weight of protein skin, 20 parts by weight of corn flakes, 5.6 parts by weight of corn meal, DDGS A raw material mixture containing 2 parts by weight, 20 parts by weight of main vein, 3 parts by weight of lupine, and 6 parts by weight of jang oil cake was prepared, and the prepared raw material mixture was steamed, followed by 0.5 parts by weight of EM probiotic, 8 parts by weight of mixed fermented feed, A complete feed containing 0.2 parts by weight of salt, 0.2 parts by weight of limestone, 2 parts by weight of CMS, and 0.2 parts by weight of fattening TMR mix was prepared, and a completely mixed feed having a moisture content of 40% was prepared, followed by fermentation in a step to be described later. A complete fermented feed can be prepared, and 12 to 14 kg of the prepared fermented feed can be fed per day.

Also, in the late stage of fattening, 7% by weight of annual ryegrass, 4% by weight of tall fescue, 11% by weight of whole cotton, 6.9% by weight of protein hull, 19.5% by weight of corn flakes, 6% by weight of corn meal, 5% by weight of DDGS, 20 ribs % by weight, lupine 5% by weight, sodium bicarbonate 0.3% by weight, salt 0.2% by weight, limestone 0.2% by weight, soy milk 6% by weight, EM probiotic 0.5% by weight, fermented feed 8% by weight, fattening TMR mix 0.4% by weight 14 to 16 kg of the completely mixed fermented feed prepared by preparing and fermenting the complete feed may be fed per day. In addition, it is possible to prepare a completely mixed fermented feed prepared by introducing high-temperature steam to prepare a completely mixed feed having a moisture content of 40% and then fermenting it in step (f) to be described later, and the prepared completely mixed fermented feed It is possible to feed 14 to 16 kg per day, whereby, rather than the effect of improving meat quantity when feeding a completely mixed fermented feed in the late stage of fattening, meat quality by improving intramuscular fat content, fatty acid composition, meat quality, flavor, meat quality and texture, etc. rating can be further improved.

In addition, even when the complete fermented feed for late fattening is fed for a short period of about 2 to 3 months before fattening, the grade of meat quality can be further improved by improving intramuscular fat, fatty acid composition, meat quality, flavor, meat tenderness and texture. can

In addition, the fully-mixed fermented feed can be supplied to Korean cattle during the breeding season, and can also be used for feeding cows for fattening, breeding, and milking due to its high content of essential amino acids.

According to a preferred embodiment of the present invention, 10% by weight of annual ryegrass, 2% by weight of tall fescue, 11% by weight of whole cotton, 13.6% by weight of protein skin, 22.90% by weight of corn flakes, jade Embryo meal 3 wt%, main vein 18 wt%, lupine 2 wt%, sodium bicarbonate 0.3 wt%, salt 0.2 wt%, limestone 0.3 wt%, soy milk cake 7 wt%, EM probiotic 0.5 wt%, fermented feed 7 wt%, A complete mixture feed containing 0.2% by weight of the fattening TMR mix can be prepared, and a complete mixture fermented feed prepared by preparing a complete mixture feed having a moisture content of 40% and then fermenting can be fed.

The cows fed with the complete blended feed fermented with the above composition in the total fattening period have good skeletal and rumen development, maximized meat volume, and improved meat quality, so that high quality Korean beef can be produced.

The step (f) is a step of preparing a perfectly formulated fermented feed by packaging the complete feed and then performing secondary fermentation.

In this step, the complete feed may be packaged using a packaging material such as a film or container made of polymer resin, thereby preventing the inflow of air into the complete feed. In the packaging, the fully mixed feed is transported to a filling machine and filled, and then measured using an automatic packaging machine to a predetermined capacity and then packed. In the case of large packaging, it is packaged in units of 100 kg or more, and it can be packed in various forms such as general packaging and large packaging (ton bag packaging).

In addition, in this step, the completely mixed feed can be prepared by secondary fermentation of the packaged complete feed, and the secondary fermentation is carried out at a temperature of 35 to 45 ° C for 2 to 10 days, followed by fermentation at 25 to 35 ° C Completely mixed fermented feed can be prepared by secondary fermentation for 3 to 10 days.

And, in this step, the temperature is adjusted so that the growth of the fermented strain in the packaged complete blend feed can proceed in the order of Bacillus strains, Lactobacillus strains, and Saccharomyces strains to prepare a high-quality completely blended fermented feed. can

This is because, when the moisture content is adjusted to 40% relative to 100 parts by weight of the completely mixed feed and the completely mixed feed is fermented under anaerobic conditions packaged in a film or sheet, the strains of the genus Bacillus and the genus Lactobacillus are rapidly It is to grow, and to balance the growth of mixed fermentation strains by allowing Bacillus strains and Lactobacillus strains to grow at a certain concentration and then Saccharomyces strains to grow, thereby improving digestibility and flavor It is possible to manufacture a complete fermented feed that has a beneficial effect on the health and growth of livestock by suppressing the growth of excellent, pathogenic bacteria.

Conventionally, in order to maintain a fermentation temperature of 35 ℃, fermented water prepared by mixing hot water heated to 100 ℃ with cooling water is supplied to feed for fermentation, but when using this method, fermented feed is It is difficult to accurately control the moisture content, and the moisture content of the fermented feed frequently exceeds 50%, resulting in a decrease in fermentation efficiency.

However, in the present invention, the high-temperature steam is injected at once to adjust the moisture content in the product to 30 to 45%, preferably, 35 to 40% even if the raw material contains its own moisture, thereby increasing the fermentation efficiency of the perfectly formulated feed. and, thereby, economic feasibility can be secured.

In addition, if the temperature of the primary workpiece is raised to 25 ° C or higher regardless of the season through the supply of high-temperature steam, self-fermentation heat of 40 ° C or higher can be generated through fermentation of the EM probiotic strain introduced during the secondary processing, As a result, fermentation can be completed within 3 days at the same time as product packaging, and stable and complete fermented feed can be produced through continuous self-fermentation thereafter.

In addition, it is possible to effectively block the growth of harmful microorganisms during steam treatment, control the fermentation rate and digestibility of raw materials, and ferment raw materials using microbial complex strains to have a beneficial effect on the health and growth of livestock, This excellent fully mixed fermented feed (fermented total mixed ration, fermented TMR) can be prepared.

Therefore, according to the method for producing a perfectly mixed fermented feed according to the present invention as described above, the moisture content of the raw material can be effectively controlled by supplying high-temperature steam at once, thereby improving fermentation efficiency and achieving economic feasibility.

In addition, it is possible to easily control the fermentation rate of raw materials, block the proliferation of harmful microorganisms, increase the rate of hydrolysis of raw materials by microbial fermentation, improve digestibility, and prepare perfectly mixed fermented feed with excellent palatability. there is.

In addition, as fermented strains and EM probiotics are introduced and fermented, the digestion rate of the feed is fast, and when fed to cattle, it helps digestion by helping the incomplete rumen function and increases productivity, and microorganisms As a result of feeding fermented feed cultured to livestock, it has the effect of increasing feed intake and reducing the smell and moisture of manure.

In addition, the completely mixed fermented feed prevents diarrhea of livestock, improves milk yield, improves meat quality, increases breeding rate, promotes growth, and metabolic disorders in the rumen by metabolites such as microorganisms that proliferate during the fermentation process and organic acids produced during the growth process. It has a preventive effect. For this reason, products containing microorganisms in livestock farming can be used as feed having functionality such as reducing feed costs for livestock farmers and increasing immunity of livestock through the development of low-cost feed products.

The prepared fully-mixed fermented feed can be used for feeding ruminant animals, and ruminant animals may be cattle, dairy cows, water buffaloes, goats, sheep, goats, or deer, but are not limited thereto, and the amount of feed fed is It can be selectively and appropriately adjusted according to the type of animal, weight, age, sex, health condition, feed ingredients, etc., and can be preferably used for feeding cows and dairy cows.

In particular, the feed may be used as a cow feed for at least one growth stage selected from the group consisting of a growing period, an early fattening period, a middle fattening period, a late fattening period, a dry period, and a breeding period, or a cow feed for milking.

Hereinafter, the present invention will be described in more detail by way of examples.

The presented examples are only specific examples of the present invention, and are not intended to limit the scope of the present invention.

<Preparation Example 1> Preparation of mixed fermented feed 1

1 ⁺⁺ grade cattle (rich super cattle) were selected, feces of the cattle were obtained in a sterilized space, and the obtained feces samples were collected and diluted 10-fold to prepare diluted feces samples. The prepared diluted fecal sample was smeared on a medium and cultured for 60 hours under anaerobic conditions at a temperature of 37° C. to obtain a first strain and a second strain producing large transparent colonies.

The medium contains 2% by weight of glucose, 2% by weight of corn starch, 5% by weight of cellulose, 5% by weight of hemicellulose, 10% by weight of pectin, 2% by weight of protein powder, 0.5% by weight of peptone, and 0.5% by weight of yeast extract. , 0.2% by weight of calcium carbonate (CaCO ₃ ), 0.01% by weight of antifoaming agent, 22.7% by weight of agar, and a pH 4 culture medium containing the rest of the water was used.

In order to identify the first strain and the second strain, the morphological characteristics were confirmed through Gram staining, spore staining, and scanning electron microscopy (JEOL, JSM-5600) analysis. As a result, the selected first strain was Gram-positive. It was confirmed that it is a Bacillus subtilis strain that is a microorganism, capable of forming endospores, and has a rod shape, and the second strain is a Gram-positive microorganism and has a rod shape. As a result of molecular biological identification and characterization of the confirmed Bacillus subtilis and Lactobacillus acidophilus, they have strong activity even under acidic medium conditions, and even under anaerobic conditions. It was confirmed that they grow well.

The Bacillus subtilis and Lactobacillus acidophilus strains isolated in the above manner were inoculated into LB medium and MRS medium, respectively, and cultured in large quantities.

Feed for culture was prepared by mixing 12 parts by weight of annuals, 10 parts by weight of whole wheat flour, 10 parts by weight of protein skin, 10 parts by weight of corn flakes, 10 parts by weight of main vein, 8 parts by weight of jang oil cake and 40 parts by weight of water. The feed was sterilized by heating at 100 °C for 5 minutes.

A mixed fermented feed was prepared by inoculating a sterilized cultured feed with a Bacillus sp. strain and a Lactobacillus sp. strain at a concentration of 1 × 10 ⁸ CFU/g, respectively, and fermenting the feed for primary fermentation at 37 ° C. for 48 hours. did

<Preparation Example 2> Preparation of mixed fermented feed 2

A feed for culture was prepared by mixing 12 parts by weight of annual, 10 parts by weight of whole cotton, 10 parts by weight of protein skin, 10 parts by weight of corn flakes, 10 parts by weight of main vein, and 8 parts by weight of jang oil cake, and the prepared feed for culture was steamed. It was introduced into the chamber, and steam was supplied at 90 ° C. and treated with steam for 30 minutes to prepare a culture feed having a water content of 40%.

A mixed fermented feed was prepared by inoculating a Bacillus sp. strain and a Lactobacillus sp. strain into a feed for culture at a concentration of 1 × 10 ⁸ CFU/g, respectively, and fermenting the feed for primary fermentation at 37 °C for 48 hours.

<Example 1>

Completely mixed fermented feed was prepared with the composition shown in Table 1 below.

Completely mixed fermented feed is a mixture of alfalfa bale, annual grass, tall fescue, timothy, soft wheat, whole cotton, cotton wheat, protein skin, corn flakes, corn meal, DDGS, main vein, almond meal, lupine, and beet pulp. prepared, and the prepared raw material mixture was treated with steam by supplying steam at 95 ° C. for 40 minutes to adjust the moisture content to 40%.

In addition, the EM probiotic is a culture solution mixed at a ratio of 4% by weight of Saccharomyces sp. strain culture, 60% by weight of corn powder, 6% by weight of molasses, 5% by weight of wheat bran and 25% by weight of water for 2 to 3 days. A fermented product fermented under anaerobic conditions maintaining a temperature of 35 ° C. was used.

Completely blended fermented feed was prepared by mixing the mixed fermented feed prepared by the method according to Preparation Example 1 in a mixing tank with the steam-treated raw material mixture, and sequentially mixing EM probiotics and additives, and the prepared completely blended feed was coated with a polyethylene film After sealed packaging, fermented at 37 ° C. for 5 days, and then fermented at 28 ° C. for 9 days to prepare a wet type fermented feed.

For reference, the fully-mixed fermented feed having the composition according to Examples 1 to 6 is obtained by adjusting the mixing amount of the raw materials to have a suitable component ratio for each growth stage of livestock when preparing the perfectly-mixed feed. The milking TMR mix used nutrients containing high amounts of vitamins A, D, and E (more than 2 million IU per kg), and the fattening TMR mix used nutrients mixed with vitamins and minerals.

<Examples 2-1 to 2-6>

A wet-type completely mixed fermented feed was prepared in the same manner as in Example 1, except for using the mixed fermented feed prepared by the method according to Preparation Example 2.

<Comparative Examples 1-1 to 1-6>

Completely mixed fermented feed was prepared in the same manner as in Example, except that the completely mixed fermented feed was prepared by adding water to the composition shown in Table 2 without steaming the feed raw material.

In addition, the EM probiotic was a fermented product fermented under the same anaerobic conditions as in Example 1, and the completely mixed feed was mixed with the raw material mixture and fermented water, mixed fermented feed prepared by the method according to the preparation example in the mixing tank, and EM It was prepared by sequentially mixing probiotics and additives, and the prepared complete feed was sealed and packed in polyethylene film, and then fermented at 37 ° C for 3 days and then fermented at 28 ° C for 5 days. Fermented feed was prepared.

<Comparative Examples 2-1 to 2-6>

A dry type fermented feed was prepared in the same manner as in Example, except that water was added to prepare the completely mixed fermented feed in the composition shown in Table 3 below.

<Comparative Examples 3-1 to 3-6>

A completely blended fermented feed was prepared in the same manner as in Example 1, except that the fermented feed was prepared by inoculating only Bacillus subtilis.

<Comparative Examples 4-1 to 4-6>

A completely mixed fermented feed was prepared in the same manner as in Example 1, except that the fermented feed was prepared by inoculating only Lactobacillus acidophilus.

<Comparative Examples 5-1 to 5-6>

Completely mixed fermented feed was prepared in the same manner as in Example 1, except that the culture feed not inoculated with Bacillus subtilis or Lactobacillus acidophilus strain was mixed with the fermented feed in the same amount.

<Experimental example>

(1) Analysis of microorganism content and characteristics of completely mixed fermented feed

The average microbial content (unit: cfu/g) and the hydrogen ion concentration (pH) of the perfectly mixed fermented feed prepared by the method according to Examples and Comparative Examples were analyzed, and the results are as follows. Table 4 shows.

As shown in Table 4, the fully mixed fermented feed prepared by the methods according to Examples 2-1 to 2-6 and Examples 1-1 to 1-6 is Bacillus subtilis ( B. subtilis ), Lactobacillus ash While it can be confirmed that the microbial content of Dophilus ( LB. acidophilus ) and Saccharomyces cerevisiae ( S. cerevisiae ) is high, the fully blended fermented feed prepared by the method according to Comparative Examples 1 to 5 It was confirmed that the microbial content was low, and in particular, the content of all inoculated Saccharomyces cerevisiae was also low.

In particular, it was confirmed that the fermented feed prepared by the method according to Examples 2-1 to 2-6 had the lowest pH and the most fermentation, but the fermented feed prepared by the method according to Comparative Examples 3 to 4 In this case, it was confirmed that not only was the amount of organic acid produced low because the change in pH was not large, but also that fermentation did not proceed smoothly.

Therefore, through the above results, it was confirmed that fermentation is performed smoothly when fermentation is performed using mixed strains, and in particular, fermentation is further promoted through steam treatment.

(2) Evaluation of quality characteristics of livestock fed with completely mixed fermented feed

The completely mixed fermented feed prepared by the method according to Examples and Comparative Examples was fed and raised to three Korean cattle, and then the quality characteristics of beef obtained from the reared Korean cattle were evaluated. The results are shown in Table 5 below. was

For reference, Example 1-1, Example 2-1, Comparative Example 1-1, Comparative Example 2-1, Comparative Example 3-1, Comparative Example 4-1 and Comparative Example 5-1 prepared by the method Completely mixed fermented feed was fed at an average of 4 to 5 kg per day to young calves aged 6 months after birth, Example 1-2, Example 2-2, Comparative Example 1-2, Comparative Example 2-2, Comparative Example 3 -2, Comparative Example 4-2 and Comparative Example 5-2, the complete mixture fermented feed prepared by the method was fed an average of 8 to 14 kg per day to 7-month-old calves, Example 1-3, Example 2-3, Comparative Example 1-3, Comparative Example 2-3, Comparative Example 3-3, Comparative Example 4-3, and Comparative Example 5-3, the fully-mixed fermented feed prepared by the method was given to a 15-month-old calf. It was fed around 14 kg on average per day, Example 1-4, Example 2-4, Comparative Example 1-4, Comparative Example 2-4, Comparative Example 3-4, Comparative Example 4-4 and Comparative Example 5-4 The completely mixed fermented feed prepared by the method according to the method was fed to calves aged 23 months of age at around 14 to 16 kg per day, and fed until 30 months of age.

The quality characteristics of beef were analyzed using beef samples obtained from cows aged 30 months, all of which were obtained from the same part of the beef, and the ratio of N-3 fatty acids and N-6 fatty acids was calculated. showed up

As shown in Table 5, as a result of analyzing the fatty acid components contained in the meat of livestock fed with the completely blended fermented feed prepared by the method according to Examples and Comparative Examples, the method according to Example 1 and Example 2 It was confirmed that the composition ratio of the prepared N-3 fatty acid and N-6 fatty acid was less than 1:10, and it was confirmed that the fatty acid content of the ideal recommended intake rate (less than 1:14) was confirmed, whereas the rest except for Comparative Example 2 It was confirmed that the fatty acid composition ratio greatly exceeded the recommended intake ratio in the case of cattle fed with the example completely mixed fermented feed.

(3) Evaluation of fecal condition characteristics

Complete mixture fermentation prepared by the method according to Example 1-5, Example 2-5, Comparative Example 1-5, Comparative Example 2-5, Comparative Example 3-5, Comparative Example 4-5 and Comparative Example 5-5 The feed was fed to a total of 35 breeding Korean cattle, 5 each, for 10 days to evaluate fecal condition characteristics such as diarrhea, and the results are shown in Table 6, and the diarrhea occurrence status was expressed as an index.

For reference, in the case of Examples 1-5 and 2-5, the feed containing protein skin at a rate of 25% by weight, which may cause loose stools or diarrhea, but containing an excessive amount of high-energy protein to breed As a feed that can be promoted, it was performed to analyze the discharge characteristics of the feed by fermentation.

In addition, the diarrhea index was evaluated by Shellman (Sherman DM, Acres SD, Sadowski PL. Springer JA, Bray B, Raybould TJ, Muscoplat CC. Protection of calves against fatal enteric colibacillosis by orally administered Echerichia coli K99-specific monoclonal antibody. Infect Immu 1983, 42; , and converted to 0 points, 1 point if the number of loose stools is more than 2 and less than 5, 2 points if the number of loose stools is more than 5 and less than 7, or 1 or more diarrhea, 3 if the number of diarrhea is 2 or more It was evaluated as a point, and it shows the average value of a total of 5 livestock.

As shown in Table 6, as a result of evaluating the defecation consistency index, in 5 cows fed the completely mixed fermented feed prepared by the method according to Example 1-5, Example 2-5 and Comparative Example 2-5 For the 2 cows, it was confirmed that 2 cows had completely normal defecation conditions and 3 cows who excreted loose stools more than once. In the case of the cows that consumed the fully formulated fermented feed inoculated with and fermented only with Saccharomyces cerevisiae, only one cow had a normal range (1 soft stool), and it was confirmed that the rest of the cows had diarrhea.

(4) Amino acid content analysis

Complete mixture fermentation prepared by the method according to Example 1-6, Example 2-6, Comparative Example 1-6, Comparative Example 2-6, Comparative Example 3-6, Comparative Example 4-6 and Comparative Example 5-6 The contents of L-arginine and L-lysine (unit mg/g) contained in the feed were analyzed, and the analysis results are shown in Table 7 below.

As shown in Table 7, in the case of the fully blended fermented feed prepared by the method according to Examples 1-6 and 2-6, the content of L- It was confirmed that arginine and L-lysine were included, and through these results, fermenting a complete feed using a complex fermentation strain can improve immunity more than using a single strain, and it is possible to improve cow fattening and It was confirmed that an excellent fully-mixed fermented feed that can greatly improve immunity while promoting fattening due to the high content of arginine and lysine, which are essential for dairy cows for breeding and milking, can be prepared.

Through the above results, it was confirmed that when using the complex strain, not only can sufficient nutrients be supplied to ruminants, but also the digestibility is improved, so that livestock fattening and milk production can be greatly increased.

Therefore, it was confirmed that a TMR feed capable of significantly improving livestock quality could be prepared through fermentation and steam treatment using a complex strain.

In particular, the prepared fully-mixed fermented feed allows Bacillus subtilis, Lactobacillus acidophilus, and Saccharomyces cerevisiae to grow in a balanced way through fermentation, and produces a large amount of organic acid, so it has high nutritional value and is suitable for the fattening period. It was confirmed that it was possible to produce cows with excellent fatty acid composition at the time of feeding, to remove indigestion factors such as diarrhea and soft stool, and to increase the production of essential amino acids, so that it could be easily used for feeding for cattle breeding.

Detailed descriptions of the preferred embodiments of the present invention disclosed as described above are provided to enable those skilled in the art to implement and practice the present invention. Although the above has been described with reference to preferred embodiments of the present invention, those skilled in the art will understand that the present invention can be variously modified and changed without departing from the scope of the present invention. For example, a person skilled in the art may use each configuration described in the above-described embodiments in a way of combining each other. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

The present invention can be embodied in other specific forms without departing from the spirit and essential characteristics of the present invention. Accordingly, the above detailed description should not be construed as limiting in all respects and should be considered as illustrative. The scope of the present invention should be determined by reasonable interpretation of the appended claims, and all changes within the equivalent scope of the present invention are included in the scope of the present invention. The invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein. In addition, claims that do not have an explicit citation relationship in the claims may be combined to form an embodiment or may be included as new claims by amendment after filing.

Claims (8)

(a) preparing a fermentation strain comprising a Bacillus subtilis strain and a Lactobacillus acidophilus strain obtained from cow feces;
(b) supplying steam at 90 ° C. and mixing the fermented strain with a feed for culture having a water content of 40% by steam treatment for 30 minutes to prepare a pre-fermentation mixed feed, the pre-fermentation mixed feed is the fermented strain Prepared by mixing at a concentration of 1 × 10 ⁴ to 1 × 10 ⁹ CFU / g, and primary fermentation to ferment for 3 to 10 days under conditions in which the temperature is maintained at 30 to 60 ° C. Preparing a mixed fermented feed;
(c) A raw material mixture including annual grass, protein hull, corn flakes, main vein, soybean oil meal, alfalfa bale, timothy, soft wheat, cotton wheat, wheat hull, almond meal, water and beet pulp by mixing the raw materials of the complete feed. Preparing;
(d) preparing a steam-treated raw material mixture by supplying steam at 80 to 130 °C to the raw material mixture for 1 to 120 minutes;
(e) mixing the steam-treated raw material mixture, the mixed fermented feed, an EM probiotic including Saccharomyces cerevisiae and an additive to prepare a complete feed; and
(f) The completely mixed feed is packaged in a film made of polymer resin, fermented at 35 to 45 ° C for 2 to 10 days, and then secondaryly fermented at 25 to 35 ° C for 3 to 10 days to obtain a completely mixed fermented feed Including; preparing a;
The EM probiotic is 1 × 10 ⁷ to 1 × 10 ⁹ CFU / g concentration of 4% by weight of Saccharomyces sp. strain culture, 60% by weight of corn meal, 6% by weight of molasses, 5% by weight of wheat bran and 25% by weight of water A method for producing a perfectly formulated fermented feed, characterized in that the fermentation product is fermented under anaerobic conditions maintaining a temperature of 25 to 35 ° C. for 2 to 3 days. delete delete delete According to claim 1,
The additive is
Salt, seasonings, sugars, flavoring agents, volatile fatty acids, Condensed Molasses Fermentation Solubles (CMS), L-ascorbic acid, choline chloride, inositol, vitamins, potassium chloride, iron citrate, magnesium oxide, amino acids, organic acids or their salts , antioxidants, fungicides, emulsifiers, pigments, potassium chloride, limestone, lincal agents, sodium bicarbonate, zinc agents, yeast, antioxidants, bypass fat and bypass protein characterized in that it comprises at least one member selected from the group consisting of Method for producing a completely blended fermented feed. According to claim 1,
The completely blended feed is a method for producing a completely blended fermented feed, characterized in that the moisture content is 30 to 45%. delete delete

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