CN111000030A - Preparation method of forest grass plant protein source probiotic fermented feed - Google Patents
Preparation method of forest grass plant protein source probiotic fermented feed Download PDFInfo
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- CN111000030A CN111000030A CN201911171198.9A CN201911171198A CN111000030A CN 111000030 A CN111000030 A CN 111000030A CN 201911171198 A CN201911171198 A CN 201911171198A CN 111000030 A CN111000030 A CN 111000030A
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- A23V2400/427—Pentosaceus
Abstract
The invention provides a preparation method of a grass plant protein source probiotic fermented feed, which relates to the technical field of feeds and comprises the following steps: (1) chopping tender branches and leaves, kneading and drying to obtain a material A; (2) drying and chopping the proteinic grass to obtain a material B; (3) mixing lactobacillus plantarum, pediococcus pentosaceus, lactobacillus acidophilus, lactobacillus fermentum and pediococcus acidilactici, and adding organic acid, short-chain fatty acid, amino acid, enzyme and vitamin to obtain probiotic stock solution; (4) and (3) mixing the material A obtained in the step (1) and the material B obtained in the step (2) to obtain a material C, adding water to dilute the probiotic stock solution obtained in the step (3), spraying the diluted probiotic stock solution to the material C, stirring uniformly, and sealing and fermenting to obtain the forest grass plant protein source probiotic fermented feed. The method has advantages of easily-accessible raw materials and low production cost, and the prepared feed has animal milk quality and milk yield.
Description
Technical Field
The invention relates to the technical field of feeds, in particular to a preparation method of a forest grass plant protein source probiotic fermented feed.
Background
The safety of food and the feeding of feed to edible animals are the key problems of general attention nowadays, and along with the rapid and large-scale development of the breeding industry in China, the problems of feed raw material shortage, meat quality reduction, environmental pollution and the like emerge endlessly, and in order to prevent the edible animals from competing for food with people and improve the meat quality, the adoption of a novel biological feed prepared from a plant protein source becomes a key research field. In the existing biological feed preparation method, common probiotics are usually used, and traditional protein sources such as soybean and alfalfa are mostly used as fermentation materials, so that the animal and human fight for grains, and the production cost is high. In recent years, broussonetia papyrifera is gradually used as a feed raw material due to rich nutrition, high protein content, and various amino acids and various trace elements required by animals, has the characteristics of fast growth, strong adaptability, wide distribution, easy reproduction, high heat, short felling period and higher economic value, and the feed prepared by fermenting the broussonetia papyrifera leaves as the main raw material does not contain pesticides and hormones, has unique faint scent, and can feed the animals with high lean meat percentage and high quality. Proteinic grass is a plant with high protein, high nutrition, high yield and high benefit, has the effects of improving immunity, resisting bacteria, promoting weight gain of animals and the like, can be prepared into powder as a high-quality protein feed source through roots, stems, leaves and the like, and is also widely concerned in recent years.
Chinese patent CN109452478A discloses an ecological pig feed fermented by paper mulberry and perilla seeds in a synergistic manner, which is prepared by the following steps: crushing fresh broussonetia papyrifera leaves and stalks of the hybrid broussonetia papyrifera to obtain broussonetia papyrifera powder; directly crushing perilla seeds to obtain perilla seed powder; mixing the paper mulberry powder and the perilla seed powder with a carbon source and mineral substances; inoculating probiotics to the mixture, fermenting, and preparing the mixture and corn, soybean meal, lysine, methionine, threonine and the like into the ecological pig compound feed. The prepared ecological pig feed is rich in nutrient substances such as small peptides, omega-3 unsaturated fatty acids, lactic acid, amino acids, vitamins, trace elements and the like, the feed raw materials are rich in natural plants, no medicinal feed additive is used, and the ecological pig feed has the characteristics of rich nutrition, easiness in absorption, high safety and low cost. However, the ecological pig compound feed comprises raw materials such as corn, soybean meal and the like, so that the animals and people compete for grains, and further the production cost is higher. Chinese patent CN109380598A discloses a paper mulberry silage and a preparation method and application thereof, comprising the following raw materials in parts by weight: 50-60 parts of paper mulberry, 10-20 parts of potato, 10-20 parts of oil seed meal, 0.05-0.1 part of overflowed enzyme and 0.5-1.5 parts of composite microecologics; the composite microecological preparation comprises saccharomyces cerevisiae, pediococcus acidilactici, lactobacillus plantarum and lactobacillus bifidus; the raw materials can cooperate with each other in the fermentation process, promote the growth of livestock and poultry, increase the daily growth of livestock and poultry, inhibit the proliferation of other harmful mixed bacteria, improve the immunity and disease resistance, comprehensively improve the disease resistance of livestock and poultry, improve the production performance and prevent and treat livestock and poultry diseases. However, the raw materials of the feed comprise potatoes and the like, and the problem of food competition between animals and people can also be caused.
Chinese patent CN107912613A discloses a feed produced by herbivore and its production method, which comprises adding plant straw at a certain ratio, cutting the plant straw; adding salt, bran and sodium bicarbonate; adding the leaf eating grass, and feeding various materials into a mixing bin for proportioning and mixing; adding EM bacteria into the mixing bin; mixing, kneading into balls; feeding the kneaded feed into a vacuum compound die; and (4) performing anaerobic fermentation on the compound feed to obtain the silage. The invention adopts the herbivore as an important raw material to produce the feed, eliminates the requirement on raw materials such as soybean, fish meal and the like with higher price, simultaneously obtains the feed with high protein content, is beneficial to producing meat and milk, improves the yield and increases the lean meat content. However, the raw materials of the invention comprise plant straws with higher specific gravity, so that the invention has relatively less comprehensive nutrition and poorer palatability.
Aiming at the problems of high production cost, low digestion and absorption utilization rate, incomplete nutrition, poor palatability and the like in feed preparation, a feed preparation method with rich raw material sources and low production cost is needed to be found, so that the feed prepared by the method not only can provide rich protein nutrition for animals, but also can enhance the immunity of the animals, promote the growth and improve the lean meat percentage and the meat taste quality.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a preparation method of a grass plant protein source probiotic fermented feed, the preparation method has rich raw material sources and low production cost, and the prepared feed can provide rich protein nutrition for animals and can improve the milk quality and the milk yield of the animals.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the invention provides a preparation method of a grass plant protein source probiotic fermented feed, which comprises the following steps:
(1) chopping tender branches and leaves, kneading and drying to obtain a material A;
(2) drying and chopping the proteinic grass to obtain a material B;
(3) mixing lactobacillus plantarum, pediococcus pentosaceus, lactobacillus acidophilus, lactobacillus fermentum and pediococcus acidilactici, and adding organic acid, short-chain fatty acid, amino acid, enzyme and vitamin to obtain probiotic stock solution;
(4) and (3) mixing the material A obtained in the step (1) and the material B obtained in the step (2) to obtain a material C, adding water to dilute the probiotic stock solution obtained in the step (3), spraying the diluted probiotic stock solution to the material C, stirring uniformly, and sealing and fermenting to obtain the forest grass plant protein source probiotic fermented feed.
Further, the tender branches and leaves in the step (1) are preferably new tender branches and leaves with the length of less than or equal to 80cm, and the source is paper mulberry or suaeda glauca; preferably a paper mulberry.
Further, the cutting in the step (1) refers to cutting the tender branches and leaves into fragments with the length of about 0.2-2cm, preferably the length of 1 cm.
Further, the drying in the step (1) is air drying until the water content is 35 to 45%, preferably 37 to 43%, and more preferably 40%.
Further, the silk kneading in the step (1) refers to kneading and softening the wood fibers of the paper mulberry broken pieces by a silk kneading machine.
Further, the proteinous grass in step (2) is a forest grass plant rich in protein, including herbivore or Russian celery; preferably Russian celery.
Further, the drying in the step (2) refers to airing or drying, specifically drying until the water content is 35 to 45%, preferably 37 to 43%, and more preferably 40%.
Further, the drying in step (2) must be completed within 8 hours, mainly due to the high protein content of the proteinous grass.
Further, the chopping in the step (2) refers to chopping the protein grass into pieces of 2-5cm, preferably 2.5-4 cm.
Further, the volume ratio of the lactobacillus plantarum, the pediococcus pentosaceus, the lactobacillus acidophilus, the lactobacillus fermentum and the pediococcus acidilactici in the step (3) is 1:1-5:1:1: 1; preferably 1:2-4:1:1: 1; further preferably 1:3:1:1: 1.
Further, the adding amount of the organic acid in the step (3) is 0.5-4% of the weight of the probiotic stock solution; preferably 0.8 to 3%; more preferably 1 to 2%.
Further, the organic acid in the step (3) is lactic acid.
Further, the adding amount of the short-chain fatty acid in the step (3) is 0.5-8% of the weight of the probiotic stock solution; preferably 1 to 5%; more preferably 1 to 2%.
Further, the short chain fatty acid in the step (3) is one or more of acetic acid, propionic acid and butyric acid.
Further, the adding amount of the amino acid in the step (3) is 0.5-5% of the weight of the probiotic stock solution; preferably 0.8 to 3%; more preferably 1 to 2%.
Further, the amino acid in the step (3) is one or more of leucine, lysine, phenylalanine, isoleucine, threonine, histidine, methionine, tryptophan and arginine.
Further, the adding amount of the enzyme in the step (3) is 0.01-0.1% of the weight of the probiotic stock solution; preferably 0.01-0.08%; more preferably 0.01 to 0.05%.
Further, the enzyme in step (3) is cellulase.
Further, the adding amount of the vitamins in the step (3) is 0.08-1% of the weight of the probiotic stock solution; preferably 0.1-0.8%; further preferably 0.1 to 0.5%.
Further, in the step (3), the vitamin is vitamin B.
Furthermore, the viable bacteria content of the probiotic stock solution in the step (3) is not less than 20 hundred million/mL.
Further, the weight ratio of the material A to the material B in the step (4) is 1-3: 1-2; preferably 1-3: 1; further preferably 1: 1.
And further, 2-3 kg of probiotic stock solution is added into each ton of the material C in the step (4).
Further, in the step (4), water is added for dilution, and the adding amount of the water is 2-3 times of the weight of the probiotic stock solution.
Further, the sealed fermentation condition in the step (4) is fermentation at 35 ℃ for 5-7 days.
In general, the pH of the fermented product is 5.0 or less, and the fermented product has a noticeable acid smell. The fermented wet feed can be stored for two years in a shady and dry place under sealed anaerobic conditions.
The technical effects obtained by the invention are as follows:
1. the invention is prepared by adopting woody plant broussonetia papyrifera tender branches and leaves with high protein and herbaceous plant proteingrass under the synergistic composition fermentation of a plurality of beneficial microorganisms without adding any other bean pulp, corn, rice bran and the like. The feed contains higher substances such as protein, free amino acid, polysaccharide, emodin, flavonoid, alkaloid, multiple vitamins, multiple trace elements and the like, and the unique mixed probiotics are used in the process, so that not only is rich protein nutrition provided for animals, but also the immunity of the animals can be enhanced, the growth can be promoted, the lean meat percentage and the meat taste quality can be improved, and the milk quality and the milk yield can be improved by feeding the animals with the feed;
2. the forest and grass protein source is rich and low in price, and can be planted in large quantities in desert lands, saline-alkali lands, stony desertification lands and waste mine and mountain miscellaneous lands in China, 1 mu of forest and grass protein can be planted to 4 mu of soybean protein (the current average yield of each mu of soybean is 123.5kg, and the protein content of soybean meal after oil extraction is about 45%);
3. the production method is simple, convenient and practical, can be used for planting and breeding at home in combination with cyclic application, can also be used for industrialized, large-scale, mechanized and standardized popularization and production, can be produced according to needs, has small investment, quick response, just-needed market, sustainable and stable benefit and small risk, and can be applied to the field of cultivation and breeding.
Detailed Description
In the invention, various strains are purchased from Beinai biology, and the names and product numbers of the various strains are respectively as follows: lactobacillus plantarum (BNCC 336421); pediococcus pentosaceus (BNCC 193259); lactobacillus acidophilus (BNCC 185342); lactobacillus fermentum (BNCC 194390); pediococcus acidilactici (BNCC 137601).
The paper mulberry is an aerospace hybrid paper mulberry, is picked in a base in Jitian agricultural company with twelve sections of reclamation of the great river east in the new region of Hangzhou cionchory in Zhejiang province within 6 months, is made of Russian celery, and is picked in a base in Jitian agricultural company with twelve sections of reclamation of the great river east in the new region of Hangzhou cionchory in Zhejiang province within 6 months.
The other raw materials are common commercial products, and thus the sources thereof are not particularly limited.
The water used in the process of the invention is tap water unless otherwise specified.
Example 1
A preparation method of a grass plant protein source probiotic fermented feed comprises the following steps:
(1) cutting tender branches and leaves of the paper mulberry with the length of 80cm into fragments with the length of 0.2cm, kneading wood fibers of the fragments of the paper mulberry by a kneading machine, airing and air-drying until the water content is 35%, and obtaining a material A;
(2) airing the proteinous grass until the water content is 35 percent, and cutting the proteinous grass into 2cm fragments to obtain a material B;
(3) mixing lactobacillus plantarum, pediococcus pentosaceus, lactobacillus acidophilus, lactobacillus fermentum and pediococcus acidilactici in a volume ratio of 1:1:1:1, and adding lactic acid, acetic acid, leucine, cellulase and vitamin B to obtain a probiotic stock solution with viable bacteria content of being equal to or larger than 20 hundred million/mL, wherein the lactic acid accounts for 0.5% of the weight of the probiotic stock solution, the acetic acid accounts for 0.5% of the weight of the probiotic stock solution, the leucine accounts for 0.5% of the weight of the probiotic stock solution, the cellulase accounts for 0.01% of the weight of the probiotic stock solution, and the vitamin B accounts for 0.08% of the weight of the probiotic stock solution;
(4) and (3) mixing the material A obtained in the step (1) and the material B obtained in the step (2) to obtain a material C, adding water to dilute the probiotic stock solution obtained in the step (3), spraying the diluted probiotic stock solution to the material C, adding 2 kilograms of probiotic stock solution to each ton of the material C, adding 2 times of water by weight of the probiotic stock solution, uniformly stirring, and carrying out sealed fermentation at 35 ℃ for 5 days to obtain the forest and grass plant protein source probiotic fermented feed.
Wherein the drying in step (2) is completed within 8 hours; in the step (4), the weight ratio of the material A to the material B is 3: 2.
Example 2
A preparation method of a grass plant protein source probiotic fermented feed comprises the following steps:
(1) cutting tender branches and leaves of the paper mulberry with the length of 80cm into fragments with the length of 2cm, rubbing and softening wood fibers of the fragments of the paper mulberry by a rubbing machine, airing and air-drying until the water content is 45%, and obtaining a material A;
(2) drying the proteinous grass until the water content is 45%, and cutting the dried proteinous grass into 5cm fragments to obtain a material B;
(3) mixing lactobacillus plantarum, pediococcus pentosaceus, lactobacillus acidophilus, lactobacillus fermentum and pediococcus acidilactici in a volume ratio of 1:5:1:1:1, and adding lactic acid, propionic acid, lysine, cellulase and vitamin B to obtain a probiotic stock solution with viable bacteria content of being equal to or larger than 20 hundred million/mL, wherein lactic acid accounts for 4% of the weight of the probiotic stock solution, acetic acid accounts for 8% of the weight of the probiotic stock solution, lysine accounts for 5% of the weight of the probiotic stock solution, cellulase accounts for 0.1% of the weight of the probiotic stock solution, and vitamin B accounts for 1% of the weight of the probiotic stock solution;
(4) and (3) mixing the material A obtained in the step (1) and the material B obtained in the step (2) to obtain a material C, adding water to dilute the probiotic stock solution obtained in the step (3), spraying the diluted probiotic stock solution to the material C, adding 3 kilograms of probiotic stock solution to each ton of the material C, adding 3 times of water by weight of the probiotic stock solution, uniformly stirring, and carrying out sealed fermentation at 35 ℃ for 7 days to obtain the forest and grass plant protein source probiotic fermented feed.
Wherein, the drying in the step (2) must be completed within 8 hours; in the step (4), the weight ratio of the material A to the material B is 1: 2.
Example 3
A preparation method of a grass plant protein source probiotic fermented feed comprises the following steps:
(1) cutting tender branches and leaves of the paper mulberry with the length of 80cm into pieces with the length of 1cm, rubbing and softening wood fibers of the paper mulberry pieces by a rubbing machine, airing and air-drying until the water content is 40% to obtain a material A;
(2) airing and air-drying the proteinic grass until the water content is 40%, and cutting the proteinic grass into 3cm fragments to obtain a material B;
(3) mixing lactobacillus plantarum, pediococcus pentosaceus, lactobacillus acidophilus, lactobacillus fermentum and pediococcus acidilactici in a volume ratio of 1:3:1:1:1, and adding lactic acid, butyric acid, phenylalanine, cellulase and vitamin B to obtain a probiotic stock solution with viable bacteria content of being equal to or larger than 20 hundred million/mL, wherein the lactic acid accounts for 1% of the weight of the probiotic stock solution, the acetic acid accounts for 1% of the weight of the probiotic stock solution, the phenylalanine accounts for 1% of the weight of the probiotic stock solution, the cellulase accounts for 0.01% of the weight of the probiotic stock solution, and the vitamin B accounts for 0.1% of the weight of the probiotic stock solution;
(4) and (3) mixing the material A obtained in the step (1) and the material B obtained in the step (2) to obtain a material C, adding water to dilute the probiotic stock solution obtained in the step (3), spraying the diluted probiotic stock solution to the material C, adding 2.5 kilograms of probiotic stock solution to each ton of the material C, adding 2.5 times of water by weight of the probiotic stock solution, uniformly stirring, and carrying out sealed fermentation at 35 ℃ for 6 days to obtain the forest grass plant protein source probiotic fermented feed.
Wherein, the drying in the step (2) must be completed within 8 hours; in the step (4), the weight ratio of the material A to the material B is 1: 1.
Example 4
A preparation method of a grass plant protein source probiotic fermented feed comprises the following steps:
(1) cutting tender branches and leaves of the paper mulberry with the length of 80cm into fragments with the length of 0.2cm, kneading wood fibers of the fragments of the paper mulberry by a kneading machine, airing and air-drying until the water content is 37%, and obtaining a material A;
(2) drying the proteinous grass until the water content is 37%, and cutting the dried proteinous grass into 2.5cm fragments to obtain a material B;
(3) mixing lactobacillus plantarum, pediococcus pentosaceus, lactobacillus acidophilus, lactobacillus fermentum and pediococcus acidilactici in a volume ratio of 1:2:1:1:1, and adding lactic acid, acetic acid, isoleucine, cellulase and vitamin B to obtain a probiotic stock solution with viable bacteria content being equal to or larger than 20 hundred million/mL, wherein the lactic acid accounts for 2% of the weight of the probiotic stock solution, the acetic acid accounts for 2% of the weight of the probiotic stock solution, the isoleucine accounts for 2% of the weight of the probiotic stock solution, the cellulase accounts for 0.05% of the weight of the probiotic stock solution, and the vitamin B accounts for 0.5% of the weight of the probiotic stock solution;
(4) and (3) mixing the material A obtained in the step (1) and the material B obtained in the step (2) to obtain a material C, adding water to dilute the probiotic stock solution obtained in the step (3), spraying the diluted probiotic stock solution to the material C, adding 2 kilograms of probiotic stock solution to each ton of the material C, adding 2 times of water by weight of the probiotic stock solution, uniformly stirring, and carrying out sealed fermentation at 35 ℃ for 5 days to obtain the forest and grass plant protein source probiotic fermented feed.
Wherein, the drying in the step (2) must be completed within 8 hours; in the step (4), the weight ratio of the material A to the material B is 1: 1.
Example 5
A preparation method of a grass plant protein source probiotic fermented feed comprises the following steps:
(1) cutting tender branches and leaves of the paper mulberry with the length of 80cm into fragments with the length of 2cm, rubbing and softening wood fibers of the fragments of the paper mulberry by a rubbing machine, airing and air-drying until the water content is 43%, and obtaining a material A;
(2) airing and air-drying the proteinic grass until the water content is 43%, and cutting the proteinic grass into 4cm fragments to obtain a material B;
(3) mixing lactobacillus plantarum, pediococcus pentosaceus, lactobacillus acidophilus, lactobacillus fermentum and pediococcus acidilactici in a volume ratio of 1:4:1:1:1, and adding lactic acid, propionic acid, threonine, cellulase and vitamin B to obtain a probiotic stock solution with viable bacteria content of being larger than or equal to 20 hundred million/mL, wherein the lactic acid accounts for 0.8% of the weight of the probiotic stock solution, the acetic acid accounts for 5% of the weight of the probiotic stock solution, the threonine accounts for 0.8% of the weight of the probiotic stock solution, the cellulase accounts for 0.08% of the weight of the probiotic stock solution, and the vitamin B accounts for 0.8% of the weight of the probiotic stock solution;
(4) and (3) mixing the material A obtained in the step (1) and the material B obtained in the step (2) to obtain a material C, adding water to dilute the probiotic stock solution obtained in the step (3), spraying the diluted probiotic stock solution to the material C, adding 3 kilograms of probiotic stock solution to each ton of the material C, adding 3 times of water by weight of the probiotic stock solution, uniformly stirring, and carrying out sealed fermentation at 35 ℃ for 7 days to obtain the forest and grass plant protein source probiotic fermented feed.
Wherein, the drying in the step (2) must be completed within 8 hours; in the step (4), the weight ratio of the material A to the material B is 3: 1.
Comparative example 1
The only difference from example 3 is that the weight ratio of material A to material B in step (4) is 1: 3.
Comparative example 2
The only difference from example 3 is that the volume ratio of Lactobacillus plantarum, Pediococcus pentosaceus, Lactobacillus acidophilus, Lactobacillus fermentum and Pediococcus acidilactici in step (3) is 0.8:6:0.8:1.2: 0.8.
Comparative example 3
The only difference from example 3 is that only material a was fermented without the addition of proteinacious grass in the manufacturing process.
Comparative example 4
The difference from example 3 is only that lactic acid is added in an amount of 0.4% by weight, butyric acid is added in an amount of 0.4% by weight, phenylalanine is added in an amount of 5.5% by weight, cellulase is added in an amount of 1.2% by weight, and vitamin B is added in an amount of 0.05% by weight.
Influence of forest grass plant protein source probiotic fermented feed on milk yield and milk quality of dairy cows
(1) Time and place of experiment: 7 months 10 days-8 months 13 days in 2019; three cattle farms, the new agriculture and dairy husbandry division of alaer, Xinjiang.
(2) And (3) experimental design: selecting Holstein cows with middle-term lactation period, similar lactation months, 1 gestation period and high yield, randomly selecting 20 cows, dividing into 10 groups, and setting 2 cows in each group, wherein 1 group is used as a control group, and the other 9 groups are used as test groups. During the test period, the control group is fed with conventional ration and mixed by a TMR machine, the test group is manually mixed by a partial silage replacing mode (10 kg of forage replaces 10kg of silage of corn stalks in each of examples 1-5 and comparative examples 1-4). Milk production, feeding and milk composition changes were recorded for the test and control groups, respectively.
(3) Milk quantity measurement and sample collection ① milk quantity measurement comprises measuring milk yield of cow for 2 days before the test, and 12 th day (early stage), 26 th day (middle stage) and 34 th day (late stage) of the test period, milking three times per day, respectively weighing milk of each cow, and accumulating total milk quantity, ② milk quality detection comprises measuring milk components and milk somatic cell number, and data detection is completed by new agricultural dairy laboratories.
TABLE 1 milk yield of cows
Note: the difference in the table is the difference between the values associated with each example group and the control group.
TABLE 2 milk cow lactoprotein Rate
Note: the difference in the table is the difference between the values associated with each example group and the control group.
TABLE 3 milk fat percentage of cows
Note: the difference in the table is the difference between the values associated with each example group and the control group.
TABLE 4 somatic cell count of cows
Note: the difference in the table is the difference between the values associated with each example group and the control group.
As can be seen from tables 1 to 4, in examples 1 to 5, although the total milk yield of the cows in the early period of the test is lower than that of the control group and the somatic cell number of the cows is higher than that of the control group, the milk yield of the cows in the middle period and the later period of the test is gradually close to or even higher than that of the control group, the somatic cell number of the cows in the middle period and the later period is gradually lower than that of the control group, and the milk lactoprotein rate and the milk lipid rate are both close to or even higher than that of the control group in the early period, the middle period and the. Therefore, the forest plant protein source probiotic fermented feed is used for partially replacing the conventional silage originally used by the dairy cow in the compound feed for feeding the dairy cow, and due to the good palatability of the forest plant protein source probiotic fermented feed, the dairy cow is accepted by the forest plant protein source probiotic fermented feed, and is favored to eat without any adverse reaction. Has obvious effect of improving milk yield, more stable milk production, higher trend of improving milk protein and milk fat rate, larger amplification and obvious effect of reducing somatic cells. Somatic Cell Count (SCC) generally refers to the number of somatic cells per ml of milk, and generally high somatic cell counts in milk result in loss of milk volume, increased microbial content, poor milk flavor, reduced nutritional value, and increased herd replacement costs. Therefore, the addition of the forest plant protein source probiotic fermented feed to the feed of the dairy cow is effective and feasible for healthy cultivation and improvement of the milk quality. Compared with the comparative examples 1-4 and the control group, the total milk yield, the milk protein rate and the milk fat rate of the four groups are all lower than the increase amount of the example 3, and the decrease amount of the somatic cell number is also lower than the decrease amount of the example 3, so that the raw materials of the feed, the strains in the fermentation process, the addition amount of the substances and the like have influence on the milk yield, the milk quality and the like of the dairy cows, and the fermented feed obtained in the protection range of the invention can improve the milk yield and the milk quality of the dairy cows.
Finally, it should be noted that the above-mentioned contents are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, and that the simple modifications or equivalent substitutions of the technical solutions of the present invention by those of ordinary skill in the art can be made without departing from the spirit and scope of the technical solutions of the present invention.
Claims (10)
1. A preparation method of a grass plant protein source probiotic fermented feed is characterized by comprising the following steps: the method comprises the following steps:
(1) chopping tender branches and leaves, kneading and drying to obtain a material A;
(2) drying and chopping the proteinic grass to obtain a material B;
(3) mixing lactobacillus plantarum, pediococcus pentosaceus, lactobacillus acidophilus, lactobacillus fermentum and pediococcus acidilactici, and adding organic acid, short-chain fatty acid, amino acid, enzyme and vitamin to obtain probiotic stock solution;
(4) and (3) mixing the material A obtained in the step (1) and the material B obtained in the step (2) to obtain a material C, adding water to dilute the probiotic stock solution obtained in the step (3), spraying the diluted probiotic stock solution to the material C, stirring uniformly, and sealing and fermenting to obtain the forest grass plant protein source probiotic fermented feed.
2. The method of manufacturing according to claim 1, wherein: the drying in the step (1) is airing and air-drying until the water content is 35-45%, and the drying in the step (2) is airing and air-drying or drying, specifically drying until the water content is 35-45%.
3. The method of manufacturing according to claim 1, wherein: the tender branches and leaves in the step (1) are new tender branches and leaves with the length of less than or equal to 80cm, and the tender branches and leaves are from paper mulberry or suaeda glauca.
4. The method of manufacturing according to claim 1, wherein: the volume ratio of the lactobacillus plantarum, the pediococcus pentosaceus, the lactobacillus acidophilus, the lactobacillus fermentum and the pediococcus acidilactici in the step (3) is 1:1-5:1: 1.
5. The method of manufacturing according to claim 1, wherein: the viable bacteria content of the probiotic stock solution in the step (3) is not less than 20 hundred million/mL.
6. The method of manufacturing according to claim 1, wherein: in the step (3), the addition amount of the organic acid is 0.5-4% of the weight of the probiotic stock solution, the addition amount of the short-chain fatty acid is 0.5-8% of the weight of the probiotic stock solution, the addition amount of the amino acid is 0.5-5% of the weight of the probiotic stock solution, the addition amount of the enzyme is 0.01-0.1% of the weight of the probiotic stock solution, and the addition amount of the vitamin is 0.08-1% of the weight of the probiotic stock solution.
7. The method of manufacturing according to claim 1, wherein: the organic acid in the step (3) is lactic acid; the short-chain fatty acid is one or more of acetic acid, propionic acid and butyric acid; the amino acid is one or more of leucine, lysine, phenylalanine, isoleucine, threonine, histidine, methionine, tryptophan and arginine; the enzyme is cellulase; the vitamin is vitamin B.
8. The method of manufacturing according to claim 1, wherein: in the step (4), the weight ratio of the material A to the material B is 1-3: 1-2.
9. The method of manufacturing according to claim 1, wherein: adding 2-3 kg of probiotic stock solution into each ton of the material C in the step (4); and adding water for dilution, wherein the adding amount of the water is 2-3 times of the weight of the probiotic stock solution.
10. The method of manufacturing according to claim 1, wherein: the sealed fermentation condition in the step (4) is fermentation at 35 ℃ for 5-7 days.
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| CN201911171198.9A Pending CN111000030A (en) | 2019-11-26 | 2019-11-26 | Preparation method of forest grass plant protein source probiotic fermented feed |
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| CN112868914A (en) * | 2021-02-04 | 2021-06-01 | 华中农业大学 | Silage rape feed and preparation method and application thereof |
| CN113598282A (en) * | 2021-08-12 | 2021-11-05 | 程栋 | Grain-free feed and manufacturing method thereof |
| CN114365794A (en) * | 2022-01-18 | 2022-04-19 | 北京平安幸福农业发展有限公司 | Functional multi-vitamin proteingrass and feeding method thereof |
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