CN109007295A - A kind of Moringa feed addictive, preparation method and purposes - Google Patents
A kind of Moringa feed addictive, preparation method and purposes Download PDFInfo
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- CN109007295A CN109007295A CN201810683211.8A CN201810683211A CN109007295A CN 109007295 A CN109007295 A CN 109007295A CN 201810683211 A CN201810683211 A CN 201810683211A CN 109007295 A CN109007295 A CN 109007295A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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Abstract
The invention discloses a kind of Moringa feed addictive, preparation method and purposes, which includes complex microorganism bacterial strain and mixed liquor, and the weight ratio of complex microorganism bacterial strain and mixed liquor is 0.1:100~1:100；Mixed liquor includes the component of following parts by weight: 30~39 parts of moringa seeds dregs of rice powder；3~8 parts of the leaf of Moringa powder of enriching gamma-aminobutyric；54~67 parts of water.Moringa feed addictive provided by the invention, which has, to be adjusted animal endocrine, promotes growth, improves quality that is immune, improving animal products and other effects, and can be improved the utilization rate of Moringa, is reduced feed cost, is avoided environmental pollution and the wasting of resources.
Description
Technical field
The present invention relates to biological feedstuff technical field more particularly to a kind of Moringa feed addictives, preparation method and purposes.
Background technique
Moringa (Moringa oleifera Lam.), also known as " drumstick tree ", originate in northern India Asia Himalaya region and
Africa is a kind of perennial tropical function tree species of Moringa.Its is full of nutrition, and dual-purpose of drug and food is known as the title of " miracle tree " comprehensively,
It is the high-quality source of the various nutrients such as vegetable protein, vitamin, folic acid, pantothenic acid, calcium, potassium, selenium.Protein, meals in leaf of Moringa
It is abundant to eat fiber, mineral matter element and vitamin content, and contains the multiple functions ingredients such as flavones, polyphenol.Moringa seeds, which remove, to be contained
Outside unsaturated fatty acid abundant, other nutrient contents such as protein are equally higher, wherein total sugar content 6.9%-
9.5%, protein is up to 36.2%-38.7%, and the content of potassium, calcium and iron reaches 515.3-617.6mg/100g, 95- respectively
135.3mg/100g and 2.9-3.5mg/100g.
However, being applied to aquatic products at present, feed is added in Moringa branch by the Moringa feed additive of livestock-raising field
In for improving livestock birds health effect, the additive amount of this mode Moringa is high, and the absorption rate of nutriment is not ideal enough.
Summary of the invention
For overcome the deficiencies in the prior art, the purpose of the present invention is to provide a kind of Moringa feed addictives, preparation side
Method and purposes, the Moringa feed addictive have adjusting animal endocrine, promote growth, raising immune, improvement animal products
Quality and other effects, and can be improved the utilization rate of Moringa, feed cost is reduced, environmental pollution and the wasting of resources are avoided.
An object of the present invention adopts the following technical scheme that realization:
A kind of Moringa feed addictive, including complex microorganism bacterial strain and mixed liquor, the complex microorganism bacterial strain and institute
The weight ratio for stating mixed liquor is 0.1:100~1:100；The mixed liquor includes the component of following parts by weight:
30~39 parts of moringa seeds dregs of rice powder；
3~8 parts of the leaf of Moringa powder of enriching gamma-aminobutyric；
54~67 parts of water.
Further, the weight ratio of the complex microorganism bacterial strain and the mixed liquor is 0.1:100, the mixed liquor packet
Include the component of following parts by weight: 38.5 parts of moringa seeds dregs of rice powder, 4 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 57.5 parts of water；
Or,
The weight ratio of the complex microorganism bacterial strain and the mixed liquor is 0.2:100, and the mixed liquor includes moringa seeds
38.5 parts of dregs of rice powder, 4 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 57.5 parts of water；Or,
The weight ratio of the complex microorganism bacterial strain and the mixed liquor is 0.5:100, and the mixed liquor includes moringa seeds
31.7 parts of dregs of rice powder, 4.8 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 63.5 parts of water；Or,
The weight ratio of the complex microorganism bacterial strain and the mixed liquor is 1:100, and the mixed liquor includes the moringa seeds dregs of rice
37 parts of powder, 7.4 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 55.6 parts of water.
Further, the complex microorganism bacterial strain is by lactobacillus acidophilus liquid, Bacillus subtillis liquid, Candida utilis ferment
Female bacterium solution compounds bacterial strain obtained according to 20~30:3 of weight ratio~5:1 ratio.
Further, the complex microorganism bacterial strain is by lactobacillus acidophilus liquid, Bacillus subtillis liquid, Candida utilis ferment
Female bacterium solution compounds bacterial strain obtained according to the ratio of weight ratio 20:3:1；Or,
The complex microorganism bacterial strain is to be pressed by lactobacillus acidophilus liquid, Bacillus subtillis liquid, candida utili bacterium solution
Bacterial strain obtained is compounded according to the ratio of weight ratio 25:3:1；Or,
The complex microorganism bacterial strain is to be pressed by lactobacillus acidophilus liquid, Bacillus subtillis liquid, candida utili bacterium solution
Bacterial strain obtained is compounded according to the ratio of weight ratio 25:5:1；Or,
The complex microorganism bacterial strain is to be pressed by lactobacillus acidophilus liquid, Bacillus subtillis liquid, candida utili bacterium solution
Bacterial strain obtained is compounded according to the ratio of weight ratio 30:5:1.
Further, every kind of bacterium solution in the lactobacillus acidophilus liquid, Bacillus subtillis liquid and candida utili bacterium solution
Fermentation density be 1010Cfu/ml to 5 × 1010cfu/ml；And/or the γ-aminobutyric acid of the Moringa feed addictive contains
Amount is 6.15mg/g.
The second object of the present invention adopts the following technical scheme that realization:
A kind of preparation method of Moringa feed addictive as described above, includes the following steps:
Moringa seeds dregs of rice powder is obtained hereinafter, crushing by water content 10% is dried to after the removal of impurities of the moringa seeds dregs of rice；
By leaf of Moringa after vacuum is protected from light processing in 100mmol/L-140mmol/L sodium chloride solution, after cleaning it is dry extremely
Water content 10% obtains the leaf of Moringa powder of enriching gamma-aminobutyric hereinafter, crushing；
Prepare complex microorganism bacterial strain；
Moringa seeds dregs of rice powder, the leaf of Moringa powder of enriching gamma-aminobutyric and water ratio in parts by weight are uniformly mixed, obtained
Mixed liquor；
It is in parts by weight the ratio of 0.1:100~1:100 by the complex microorganism bacterial strain and the mixed liquor
It ferments under the conditions of one, obtains Moringa feed addictive wet-milling；；
The Moringa feed addictive wet-milling is spray-dried under a second condition, obtains Moringa feed addictive.
Further, it is described by the complex microorganism bacterial strain and the mixed liquor be in parts by weight 0.1:100~
The ratio of 1:100 further includes following steps before being fermented in the first condition:
The mixed liquor is sterilized at the first temperature, is cooled to room temperature；First temperature is 85-120 DEG C.
It is further, described to prepare complex microorganism bacterial strain specifically:
It is 10 by the fermentation density of bacterium solution10Cfu/ml to 5 × 1010The lactobacillus acidophilus liquid of cfu/ml, Bacillus subtillis
Liquid and candida utili bacterium solution compound to obtain the complex microorganism bacterial strain by weight 20~30:3~5:1 ratio.
Further, it is 30 DEG C -40 DEG C that the first condition, which includes: fermentation temperature, and fermentation pH value is 6.4-6.7, fermentation
Time is -9 days 6 days；And/or the second condition include: atomisation pressure be 0.3-0.6MPa, charging rate 0.15-
0.5m/s, hot blast temperature are 120-150 DEG C.
The third object of the present invention adopts the following technical scheme that realization:
A kind of purposes of Moringa feed addictive as described above, the Moringa feed addictive be applied to aquatic feeds or
The additive agent field of feed is supported in herding.
Compared with prior art, the beneficial effects of the present invention are:
It is obtained after dried, crushed using the leaf of Moringa handled in 100mmol/L-140mmol/L sodium chloride solution
Moringa feed addictive made from the leaf of Moringa powder of enriching gamma-aminobutyric, is enriched γ-aminobutyric acid, not only has and adjusts
Animal endocrine promotes to grow, improve immune and resisting stress and other effects, moreover it is possible to improve the quality of animal products.Secondly as making
Not only full of nutrition with the moringa seeds powder obtained after dried, crushed, protein content is high, contains the functionality such as flavones, polyphenol
Ingredient, it is immune and other effects with anti-oxidant, anti-inflammatory and enhancing, growth of animals or poultry can be improved, improve livestock and poultry production performance, Slaughter
And meat；And can be improved the utilization rate of Moringa, feed cost is reduced, environmental pollution and the wasting of resources are avoided.This
Outside, using there is the fermentation of complex microorganism bacterial strain, it is easy that the substance absorbed and anti-nutritional factors forwarding can will be difficult in leaf of Moringa
In nutriments such as free amino acid, the micromolecule polypeptides that animal absorbs.The Moringa feed addictive raw material are environmentally protective, at
The Moringa feed addictive is added in feed and feeds by the ingredients such as product mycetome albumen, free amino acid, probiotics, active peptide
Animal can improve feed palatability, and livestock and poultry is promoted to digest and assimilate, and improve immunity, improve intestinal environment health and promote growth.
Specific embodiment
In the following, being described further in conjunction with specific embodiment to the present invention, it should be noted that is do not collided
Under the premise of, new embodiment can be formed between various embodiments described below or between each technical characteristic in any combination.
Moringa feed addictive provided in an embodiment of the present invention, including complex microorganism bacterial strain and mixed liquor, it is described compound
The weight ratio of microbial strains and the mixed liquor is 0.1:100~1:100；The mixed liquor includes the group of following parts by weight
Point: 30~39 parts of moringa seeds dregs of rice powder；3~8 parts of the leaf of Moringa powder of enriching gamma-aminobutyric；54~67 parts of water.
It should be understood that γ-aminobutyric acid (γ-aminobutyric acid, GABA) is a kind of nonprotein function ammonia
Base acid in blood pressure lowering, diuresis, promotes sleep, enhancement brain vigor, delays brain aging, trophic nerve cell and promotes liver kidney function
Etc. have important physiological function.In livestock and poultry breeding, feed of the feeding containing γ-aminobutyric acid, which is adjusted in animal, divides
It secretes, promotes growth, improves immune and resisting stress level, change and improve the quality including animal products.It should be noted that enrichment
The leaf of Moringa powder of γ-aminobutyric acid is acidified leaf of Moringa, anoxic, high-salt stress, cold stimulation, thermostimulation and mechanical stimulus etc.
After environment stress environment carries out enrichment processing to γ-aminobutyric acid, then drying, the obtained powder of crushing.
Moringa feed addictive provided by the above embodiment uses in 100mmol/L-140mmol/L sodium chloride solution
Moringa feed addictive made from the leaf of Moringa powder for the enriching gamma-aminobutyric that the leaf of Moringa of reason obtains after dried, crushed, it is rich
γ-aminobutyric acid is collected, has not only had and adjust animal endocrine, promote growth, improve immune and resisting stress and other effects, moreover it is possible to change
The quality of kind animal products.Secondly, the by-product that the moringa seeds dregs of rice extract oil as Moringa, is a kind of important renewable biomass money
Source.Moringa seeds powder will be obtained after the drying of the moringa seeds dregs of rice, crushing, is used to prepare Moringa feed addictive, not only full of nutrition, albumen
Matter content is high, immune and other effects with anti-oxidant, anti-inflammatory and enhancing containing functional components such as flavones, polyphenol, can improve livestock and poultry
Growth improves livestock and poultry production performance, Slaughter and meat；And can be improved the utilization rate of Moringa, reduce feed at
This, environmental pollution and the wasting of resources caused by avoiding the moringa seeds dregs of rice due to being simply discarded.In addition, using there is complex microorganism
Strain fermentation, the free amine group that animal absorption can be easy to by the substance absorbed and anti-nutritional factors forwarding is difficult in leaf of Moringa
The nutriments such as acid, micromolecule polypeptide.The Moringa feed addictive raw material are environmentally protective, finished product mycetome albumen, free ammonia
The Moringa feed addictive is added nutrition purposes, especially for feeding animals in feed, it is agreeable to the taste can to improve feed by the ingredients such as base acid, probiotics, active peptide
Property, promote livestock and poultry to digest and assimilate, improve immunity, improves intestinal environment health and promote growth.
The weight ratio of preferably embodiment, the complex microorganism bacterial strain and the mixed liquor is 0.1:100, institute
State the component that mixed liquor includes following parts by weight: 38.5 parts of moringa seeds dregs of rice powder, 4 parts of the leaf of Moringa powder of enriching gamma-aminobutyric,
57.5 parts of water；Or,
The weight ratio of the complex microorganism bacterial strain and the mixed liquor is 0.2:100, and the mixed liquor includes moringa seeds
38.5 parts of dregs of rice powder, 4 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 57.5 parts of water；Or,
The weight ratio of the complex microorganism bacterial strain and the mixed liquor is 0.5:100, and the mixed liquor includes moringa seeds
31.7 parts of dregs of rice powder, 4.8 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 63.5 parts of water；Or,
The weight ratio of the complex microorganism bacterial strain and the mixed liquor is 1:100, and the mixed liquor includes the moringa seeds dregs of rice
37 parts of powder, 7.4 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 55.6 parts of water.
Preferably embodiment, the complex microorganism bacterial strain be by lactobacillus acidophilus liquid, Bacillus subtillis liquid,
Candida utili bacterium solution compounds bacterial strain obtained according to 20~30:3 of weight ratio~5:1 ratio.
Preferably embodiment, the complex microorganism bacterial strain be by lactobacillus acidophilus liquid, Bacillus subtillis liquid,
Candida utili bacterium solution compounds bacterial strain obtained according to the ratio of weight ratio 20:3:1；Or,
The complex microorganism bacterial strain is to be pressed by lactobacillus acidophilus liquid, Bacillus subtillis liquid, candida utili bacterium solution
Bacterial strain obtained is compounded according to the ratio of weight ratio 25:3:1；Or,
The complex microorganism bacterial strain is to be pressed by lactobacillus acidophilus liquid, Bacillus subtillis liquid, candida utili bacterium solution
Bacterial strain obtained is compounded according to the ratio of weight ratio 25:5:1；Or,
The complex microorganism bacterial strain is to be pressed by lactobacillus acidophilus liquid, Bacillus subtillis liquid, candida utili bacterium solution
Bacterial strain obtained is compounded according to the ratio of weight ratio 30:5:1.
Preferably embodiment, the lactobacillus acidophilus liquid, Bacillus subtillis liquid and candida utili bacterium solution
In every kind of bacterium solution fermentation density be 1010Cfu/ml to 5 × 1010cfu/ml；And/or the γ-of the Moringa feed addictive
Gamma-propalanine content is 6.15mg/g.
A kind of preparation method of Moringa feed addictive as described above, includes the following steps:
Water content 10% will be dried to hereinafter, being crushed to 100-200 mesh obtains moringa seeds dregs of rice powder after the removal of impurities of the moringa seeds dregs of rice；
By leaf of Moringa after vacuum is protected from light processing in 100mmol/L-140mmol/L sodium chloride solution, after cleaning it is dry extremely
Water content 10% obtains the leaf of Moringa powder of enriching gamma-aminobutyric hereinafter, being crushed to 100-200 mesh.
Prepare complex microorganism bacterial strain: the complex microorganism bacterial strain include lactobacillus acidophilus liquid, Bacillus subtillis liquid,
Candida utili bacterium solution.Different strains are individually cultivated, and fermentation density is 1010Cfu/ml to 5 × 1010cfu/ml.Condition of culture
Are as follows: cultivation temperature is 30-40 DEG C, and culture revolving speed is 120-150r/min, incubation time 18h-36h.
Moringa seeds dregs of rice powder, the leaf of Moringa powder of enriching gamma-aminobutyric and water ratio in parts by weight are uniformly mixed, obtained
Mixed liquor.
It is in parts by weight the ratio of 0.1:100~1:100 by the complex microorganism bacterial strain and the mixed liquor
It ferments under the conditions of one, obtains Moringa feed addictive wet-milling.
The Moringa feed addictive wet-milling is spray-dried under a second condition, obtains Moringa feed addictive.
Moringa is under the environment stresses environment such as acidification, anoxic, high-salt stress, cold stimulation, thermostimulation and mechanical stimulus, meeting
There is the phenomenon that γ-aminobutyric acid accumulation, the embodiment of the present invention utilizes high-salt stress enriching gamma-aminobutyric, specific: by Moringa
Leaf vacuum in 100mmol/L-140mmol/L sodium chloride solution is protected from light processing 8-12h, and enrichment γ-ammonia is obtained after drying and crushing
The leaf of Moringa powder of base butyric acid.
The complex microorganism bacterial strain is by preferably embodiment in parts by weight with the mixed liquor described
The ratio of 0.1:100~1:100 further includes following steps before being fermented in the first condition:
The mixed liquor is sterilized at the first temperature, is cooled to room temperature；First temperature is 85-120 DEG C.
Preferably embodiment, it is described to prepare complex microorganism bacterial strain specifically:
It is 10 by the fermentation density of bacterium solution10Cfu/ml to 5 × 1010The lactobacillus acidophilus liquid of cfu/ml, Bacillus subtillis
Liquid and candida utili bacterium solution compound to obtain the complex microorganism bacterial strain by weight 20~30:3~5:1 ratio.
Preferably embodiment, the first condition include: that fermentation temperature is 30 DEG C -40 DEG C, and fermentation pH value is
6.4-6.7, fermentation time are -9 days 6 days；And/or the second condition includes: that atomisation pressure is 0.3-0.6MPa, feeds speed
Degree is 0.15-0.5m/s, and hot blast temperature is 120-150 DEG C.
A kind of purposes of Moringa feed addictive as described above, the Moringa feed addictive be applied to aquatic feeds or
The additive agent field of feed is supported in herding.
Embodiment 1
Moringa feed addictive in the embodiment includes complex microorganism bacterial strain and mixed liquor, the composite microbial bacteria
The weight ratio of strain and the mixed liquor is 0.1:100, and the mixed liquor includes the component of following parts by weight: moringa seeds dregs of rice powder
38.5 parts, 4 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 57.5 parts of water.
The moringa seeds dregs of rice are removed into the impurity such as stone grain, moisture content 10% is dried to hereinafter, being crushed to 100 mesh, obtains Moringa
Seed dregs of rice powder, it is spare.The leaf of Moringa of the fresh mature of picking is removed into the sundries on surface, is washed with deionized, room temperature is dried.It will
Leaf of Moringa after drying is placed in 100mmol/L sodium chloride solution, in vacuum and is protected from light lower processing 10h, is washed with deionized water
Forced air drying to moisture content is lower than 10% at 50 DEG C afterwards, and crushing sieves with 100 mesh sieve to obtain the leaf of Moringa of enriching gamma-aminobutyric
Powder.
Prepare complex microorganism bacterial strain: the complex microorganism bacterial strain include lactobacillus acidophilus liquid, Bacillus subtillis liquid,
Candida utili bacterium solution.Different strains are individually cultivated, and fermentation density is 1010Cfu/ml to 5 × 1010cfu/ml.Condition of culture
Are as follows: cultivation temperature is 30 DEG C, and culture revolving speed is 120r/min, incubation time 18h.Lactobacillus acidophilus liquid, Ko subtilis bar
Bacterium solution, candida utili bacterium solution are mixed to get complex microorganism bacterial strain according to the ratio of weight ratio 20:3:1.
By 38.5 parts of moringa seeds dregs of rice powder, 4 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 57.5 parts of water are uniformly mixed, and are obtained
Mixed liquor.
After mixed liquor is sterilized at a temperature of 105 DEG C, it is cooled to room temperature.The complex microorganism bacterial strain with it is sterilized
Mixed liquor afterwards is that the ratio of 0.1:100 is fermented in the fermenter in parts by weight, obtains Moringa feed addictive wet-milling.
Wherein, fermentation temperature is 30 DEG C, and fermentation pH value is 6.4, and fermentation time is 6 days.
It will be spray-dried after the concentration of above-mentioned Moringa feed addictive wet-milling homogeneous to get Moringa feed addictive.Its
In, atomisation pressure 0.3MPa, charging rate 0.1m/s, hot blast temperature are 135 DEG C.
Alpha-aminobutyric acid content measurement: it is used using high effective liquid chromatography for measuring without the peppery of enriching gamma-aminobutyric
Moringa feed addictive prepared by the Moringa feed addictive of the wooden leaf preparation and the leaf of Moringa of use enriching gamma-aminobutyric
Alpha-aminobutyric acid content.Wherein, chromatographic column uses C18 column (250mm × 4.60mm, 5 μm), Detection wavelength 436nm, column temperature
It is 30 DEG C, sample volume is 10 μ L, and mobile phase is acetonitrile and ammonium acetate mixed solution.After measured, the not enriched gamma-amino fourth of use
The Moringa feed addictive of the leaf of Moringa preparation of acid and the Moringa feed for using the leaf of Moringa of enriching gamma-aminobutyric to prepare add
The alpha-aminobutyric acid content for adding agent is respectively 1.91mg/g and 6.15mg/g, and alpha-aminobutyric acid content improves nearly 3 times.
Embodiment 2
Moringa feed addictive in the embodiment includes complex microorganism bacterial strain and mixed liquor, the composite microbial bacteria
The weight ratio of strain and the mixed liquor is 0.2:100, and the mixed liquor includes 38.5 parts of moringa seeds dregs of rice powder, is enriched with gamma-amino fourth
4 parts of leaf of Moringa powder, 57.5 parts of water of acid.
The moringa seeds dregs of rice are removed into the impurity such as stone grain, moisture content 10% is dried to hereinafter, being crushed to 100 mesh, obtains Moringa
Seed dregs of rice powder, it is spare.The leaf of Moringa of the fresh mature of picking is removed into the sundries on surface, is washed with deionized, room temperature is dried.It will
Leaf of Moringa after drying is placed in 120mmol/L sodium chloride solution, in vacuum and is protected from light lower processing 10h, is washed with deionized water
Forced air drying to moisture content is lower than 10% at 50 DEG C afterwards, and crushing sieves with 100 mesh sieve to obtain the leaf of Moringa of enriching gamma-aminobutyric
Powder.
Prepare complex microorganism bacterial strain: the complex microorganism bacterial strain include lactobacillus acidophilus liquid, Bacillus subtillis liquid,
Candida utili bacterium solution.Different strains are individually cultivated, and fermentation density is 1010Cfu/ml to 5 × 1010cfu/ml.Condition of culture
Are as follows: cultivation temperature is 37 DEG C, and culture revolving speed is 120r/min, incubation time 18h.Lactobacillus acidophilus liquid, Ko subtilis bar
Bacterium solution, candida utili bacterium solution are mixed to get complex microorganism bacterial strain according to the ratio of weight ratio 25:3:1.
By 38.5 parts of moringa seeds dregs of rice powder, 4 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 57.5 parts of water are uniformly mixed, and are obtained
Mixed liquor.
After mixed liquor is sterilized at a temperature of 105 DEG C, it is cooled to room temperature.The complex microorganism bacterial strain with it is sterilized
Mixed liquor afterwards is that the ratio of 0.2:100 is fermented in the fermenter in parts by weight, obtains Moringa feed addictive wet-milling.
Wherein, fermentation temperature is 37 DEG C, and fermentation pH value is 6.5, and fermentation time is 6 days.
It will be spray-dried after the concentration of above-mentioned Moringa feed addictive wet-milling homogeneous to get Moringa feed addictive.Its
In, atomisation pressure 0.4MPa, charging rate 0.15m/s, hot blast temperature are 135 DEG C.
Alpha-aminobutyric acid content measurement: it is used using high effective liquid chromatography for measuring without the peppery of enriching gamma-aminobutyric
Moringa feed addictive prepared by the Moringa feed addictive of the wooden leaf preparation and the leaf of Moringa of use enriching gamma-aminobutyric
Alpha-aminobutyric acid content.Wherein, chromatographic column uses C18 column (250mm × 4.60mm, 5 μm), Detection wavelength 436nm, column temperature
It is 30 DEG C, sample volume is 10 μ L, and mobile phase is acetonitrile and ammonium acetate mixed solution.After measured, the not enriched gamma-amino fourth of use
The Moringa feed addictive of the leaf of Moringa preparation of acid and the Moringa feed for using the leaf of Moringa of enriching gamma-aminobutyric to prepare add
The alpha-aminobutyric acid content for adding agent is respectively 1.85mg/g and 7.93mg/g, and alpha-aminobutyric acid content improves nearly 4 times.
Embodiment 3
Moringa feed addictive in the embodiment includes complex microorganism bacterial strain and mixed liquor, the composite microbial bacteria
The weight ratio of strain and the mixed liquor is 0.5:100, and the mixed liquor includes 31.7 parts of moringa seeds dregs of rice powder, is enriched with gamma-amino fourth
4.8 parts of leaf of Moringa powder, 63.5 parts of water of acid.
The moringa seeds dregs of rice are removed into the impurity such as stone grain, moisture content 10% is dried to hereinafter, being crushed to 100 mesh, obtains Moringa
Seed dregs of rice powder, it is spare.The leaf of Moringa of the fresh mature of picking is removed into the sundries on surface, is washed with deionized, room temperature is dried.It will
Leaf of Moringa after drying is placed in 130mmol/L sodium chloride solution, in vacuum and is protected from light lower processing 10h, is washed with deionized water
Forced air drying to moisture content is lower than 10% at 50 DEG C afterwards, and crushing sieves with 100 mesh sieve to obtain the leaf of Moringa of enriching gamma-aminobutyric
Powder.
Prepare complex microorganism bacterial strain: the complex microorganism bacterial strain include lactobacillus acidophilus liquid, Bacillus subtillis liquid,
Candida utili bacterium solution.Different strains are individually cultivated, and fermentation density is 1010Cfu/ml to 5 × 1010cfu/ml.Condition of culture
Are as follows: cultivation temperature is 37 DEG C, and culture revolving speed is 150r/min, and incubation time is for 24 hours.Lactobacillus acidophilus liquid, Ko subtilis bar
Bacterium solution, candida utili bacterium solution are mixed to get complex microorganism bacterial strain according to the ratio of weight ratio 25:5:1.
By 31.7 parts of moringa seeds dregs of rice powder, 4.8 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 63.5 parts of water are uniformly mixed, and are obtained
To mixed liquor.
After mixed liquor is sterilized at a temperature of 105 DEG C, it is cooled to room temperature.The complex microorganism bacterial strain with it is sterilized
Mixed liquor afterwards is that the ratio of 0.5:100 is fermented in the fermenter in parts by weight, obtains Moringa feed addictive wet-milling.
Wherein, fermentation temperature is 37 DEG C, and fermentation pH value is 6.7, and fermentation time is 8 days.
It will be spray-dried after the concentration of above-mentioned Moringa feed addictive wet-milling homogeneous to get Moringa feed addictive.Its
In, atomisation pressure 0.55MPa, charging rate 0.25m/s, hot blast temperature are 150 DEG C.
Alpha-aminobutyric acid content measurement: it is used using high effective liquid chromatography for measuring without the peppery of enriching gamma-aminobutyric
Moringa feed addictive prepared by the Moringa feed addictive of the wooden leaf preparation and the leaf of Moringa of use enriching gamma-aminobutyric
Alpha-aminobutyric acid content.Wherein, chromatographic column uses C18 column (250mm × 4.60mm, 5 μm), Detection wavelength 436nm, column temperature
It is 30 DEG C, sample volume is 10 μ L, and mobile phase is acetonitrile and ammonium acetate mixed solution.After measured, the not enriched gamma-amino fourth of use
The Moringa feed addictive of the leaf of Moringa preparation of acid and the Moringa feed for using the leaf of Moringa of enriching gamma-aminobutyric to prepare add
The alpha-aminobutyric acid content for adding agent is respectively 2.15mg/g and 9.10mg/g, and alpha-aminobutyric acid content improves nearly 4.5 times.
Embodiment 4
Moringa feed addictive in the embodiment includes complex microorganism bacterial strain and mixed liquor, the composite microbial bacteria
The weight ratio of strain and the mixed liquor is 1:100, and the mixed liquor includes 37 parts of moringa seeds dregs of rice powder, enriching gamma-aminobutyric
7.4 parts of leaf of Moringa powder, 55.6 parts of water.
The moringa seeds dregs of rice are removed into the impurity such as stone grain, moisture content 10% is dried to hereinafter, being crushed to 100 mesh, obtains Moringa
Seed dregs of rice powder, it is spare.The leaf of Moringa of the fresh mature of picking is removed into the sundries on surface, is washed with deionized, room temperature is dried.It will
Leaf of Moringa after drying is placed in 140mmol/L sodium chloride solution, in vacuum and is protected from light lower processing 10h, is washed with deionized water
Forced air drying to moisture content is lower than 10% at 50 DEG C afterwards, and crushing sieves with 100 mesh sieve to obtain the leaf of Moringa of enriching gamma-aminobutyric
Powder.
Prepare complex microorganism bacterial strain: the complex microorganism bacterial strain include lactobacillus acidophilus liquid, Bacillus subtillis liquid,
Candida utili bacterium solution.Different strains are individually cultivated, and fermentation density is 1010Cfu/ml to 5 × 1010cfu/ml.Condition of culture
Are as follows: cultivation temperature is 35 DEG C, and culture revolving speed is 150r/min, incubation time 27h.Lactobacillus acidophilus liquid, Ko subtilis bar
Bacterium solution, candida utili bacterium solution are mixed to get complex microorganism bacterial strain according to the ratio of weight ratio 30:5:1.
By 37 parts of moringa seeds dregs of rice powder, 7.4 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 55.6 parts of water are uniformly mixed, and are obtained
Mixed liquor.
After mixed liquor is sterilized at a temperature of 105 DEG C, it is cooled to room temperature.The complex microorganism bacterial strain with it is sterilized
Mixed liquor afterwards is that the ratio of 1:100 is fermented in the fermenter in parts by weight, obtains Moringa feed addictive wet-milling.Its
In, fermentation temperature is 35 DEG C, and fermentation pH value is 6.7, and fermentation time is 9 days.
It will be spray-dried after the concentration of above-mentioned Moringa feed addictive wet-milling homogeneous to get Moringa feed addictive.Its
In, atomisation pressure 0.5MPa, charging rate 0.25m/s, hot blast temperature are 140 DEG C.
Alpha-aminobutyric acid content measurement: it is used using high effective liquid chromatography for measuring without the peppery of enriching gamma-aminobutyric
Moringa feed addictive prepared by the Moringa feed addictive of the wooden leaf preparation and the leaf of Moringa of use enriching gamma-aminobutyric
Alpha-aminobutyric acid content.Wherein, chromatographic column uses C18 column (250mm × 4.60mm, 5 μm), Detection wavelength 436nm, column temperature
It is 30 DEG C, sample volume is 10 μ L, and mobile phase is acetonitrile and ammonium acetate mixed solution.After measured, the not enriched gamma-amino fourth of use
The Moringa feed addictive of the leaf of Moringa preparation of acid and the Moringa feed for using the leaf of Moringa of enriching gamma-aminobutyric to prepare add
The alpha-aminobutyric acid content for adding agent is respectively 2.03mg/g and 11.25mg/g, and alpha-aminobutyric acid content improves nearly 5.5 times.
Application test
The resulting Moringa feed addictive of embodiment 1 to 4 is being improved into efficiency of feed utilization, is improving answering in animal performance
With.
Landrace 208 that pig age, weight, health status are almost the same are selected, are randomly divided into 13 groups, every group of boar, mother
Pig each 8.Wherein: (1) control group feeds basal feed；(2) the corresponding feeding basis of test group 1, test group 2 and test group 3 is raised
The resulting Moringa feed addictive of 2%, 5% and 8% embodiment 1 is added in material；(3) test group 4, test group 5 and test group 6 are right
Answer the addition resulting Moringa feed addictive of 2%, 5% and 8% embodiment 2 in feeding basal feed；(4) test group 7, test group
8 and the corresponding feeding basal feed of test group 9 in add the resulting Moringa feed addictive of 2%, 5% and 8% embodiment 3；(5) it tries
It tests in the corresponding feeding basal feed of group 10, test group 11 and test group 12 and adds the resulting Moringa of 2%, 5% and 8% embodiment 4
Feed addictive.During test, test pig is freely eaten, drinks water, and pig house keeps good comfortable hygienic conditions, and to test pig
Feeding, disease etc. observed and recorded.Empty stomach weighing is carried out to pig of participating in the experiment after feeding 60 days, calculates average feed intake and feed
Ratio is converted, test result is as shown in table 1.
Table 1
Table 1 is influence of the Moringa feed addictive to the growth indexes of test pig.As can be seen from the table, add into feed
Add a certain amount of Moringa feed addictive that can increase the average daily gain of test pig, reduce food conversion when disease incident,
And 5% additive amount is best to the application effect of test pig.Compared with feeding basal feed, Moringa feed addictive can be mentioned
High pig adjusts animal endocrine to the utilization rate of feed, and livestock and poultry is promoted to digest and assimilate, and improves immunity, promotes the growth of pig, has
There is great application prospect.
The above embodiment is only the preferred embodiment of the present invention, and the scope of protection of the present invention is not limited thereto,
The variation and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention
Claimed range.
Claims (10)
1. a kind of Moringa feed addictive, which is characterized in that including complex microorganism bacterial strain and mixed liquor, the complex microorganism
The weight ratio of bacterial strain and the mixed liquor is 0.1:100~1:100；The mixed liquor includes the component of following parts by weight:
30~39 parts of moringa seeds dregs of rice powder；
3~8 parts of the leaf of Moringa powder of enriching gamma-aminobutyric；
54~67 parts of water.
2. Moringa feed addictive as described in claim 1, which is characterized in that the complex microorganism bacterial strain and the mixing
The weight ratio of liquid is 0.1:100, and the mixed liquor includes the component of following parts by weight: 38.5 parts of moringa seeds dregs of rice powder, is enriched with γ-
4 parts of the leaf of Moringa powder of aminobutyric acid, 57.5 parts of water；Or,
The weight ratio of the complex microorganism bacterial strain and the mixed liquor is 0.2:100, and the mixed liquor includes moringa seeds dregs of rice powder
38.5 parts, 4 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 57.5 parts of water；Or,
The weight ratio of the complex microorganism bacterial strain and the mixed liquor is 0.5:100, and the mixed liquor includes moringa seeds dregs of rice powder
31.7 parts, 4.8 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 63.5 parts of water；Or,
The weight ratio of the complex microorganism bacterial strain and the mixed liquor is 1:100, and the mixed liquor includes moringa seeds dregs of rice powder 37
Part, 7.4 parts of the leaf of Moringa powder of enriching gamma-aminobutyric, 55.6 parts of water.
3. Moringa feed addictive as claimed in claim 1 or 2, which is characterized in that the complex microorganism bacterial strain is by thermophilic
Lactobacillus lactis liquid, Bacillus subtillis liquid, candida utili bacterium solution are compounded according to 20~30:3 of weight ratio~5:1 ratio and are made
The bacterial strain obtained.
4. Moringa feed addictive as claimed in claim 3, which is characterized in that the complex microorganism bacterial strain is by acidophilus cream
Bacillus liquid, Bacillus subtillis liquid, candida utili bacterium solution compound bacterial strain obtained according to the ratio of weight ratio 20:3:1；
Or,
The complex microorganism bacterial strain is by lactobacillus acidophilus liquid, Bacillus subtillis liquid, candida utili bacterium solution according to weight
It measures the ratio than 25:3:1 and compounds bacterial strain obtained；Or,
The complex microorganism bacterial strain is by lactobacillus acidophilus liquid, Bacillus subtillis liquid, candida utili bacterium solution according to weight
It measures the ratio than 25:5:1 and compounds bacterial strain obtained；Or,
The complex microorganism bacterial strain is by lactobacillus acidophilus liquid, Bacillus subtillis liquid, candida utili bacterium solution according to weight
It measures the ratio than 30:5:1 and compounds bacterial strain obtained.
5. Moringa feed addictive as claimed in claim 4, which is characterized in that the lactobacillus acidophilus liquid, Ko subtilis bar
The fermentation density of every kind of bacterium solution is 10 in bacterium solution and candida utili bacterium solution10Cfu/ml to 5 × 1010cfu/ml；And/or institute
The alpha-aminobutyric acid content for stating Moringa feed addictive is 6.15mg/g.
6. a kind of preparation method of Moringa feed addictive as described in any one in claim 1-5, which is characterized in that including such as
Lower step:
Moringa seeds dregs of rice powder is obtained hereinafter, crushing by water content 10% is dried to after the removal of impurities of the moringa seeds dregs of rice；
By leaf of Moringa after vacuum is protected from light processing in 100mmol/L-140mmol/L sodium chloride solution, dry after cleaning to aqueous
Amount 10% obtains the leaf of Moringa powder of enriching gamma-aminobutyric hereinafter, crushing；
Prepare complex microorganism bacterial strain；
Moringa seeds dregs of rice powder, the leaf of Moringa powder of enriching gamma-aminobutyric and water ratio in parts by weight are uniformly mixed, mixed
Liquid；
It is in parts by weight the ratio of 0.1:100~1:100 at first by the complex microorganism bacterial strain and the mixed liquor
It ferments under part, obtains Moringa feed addictive wet-milling；
The Moringa feed addictive wet-milling is spray-dried under a second condition, obtains Moringa feed addictive.
7. the preparation method of Moringa feed addictive as claimed in claim 6, which is characterized in that it is described will be described compound micro-
Biological bacterial strain and the mixed liquor are before the ratio of 0.1:100~1:100 is fermented in the first condition in parts by weight
Further include following steps:
The mixed liquor is sterilized at the first temperature, is cooled to room temperature；First temperature is 85-120 DEG C.
8. the preparation method of Moringa feed addictive as claimed in claim 6, which is characterized in that described to prepare complex microorganism
Bacterial strain specifically:
It is 10 by the fermentation density of bacterium solution10Cfu/ml to 5 × 1010The lactobacillus acidophilus liquid of cfu/ml, Bacillus subtillis liquid and
Candida utili bacterium solution compounds to obtain the complex microorganism bacterial strain by weight 20~30:3~5:1 ratio.
9. the preparation method of Moringa feed addictive as claimed in claim 6, which is characterized in that the first condition includes:
Fermentation temperature is 30 DEG C -40 DEG C, and fermentation pH value is 6.4-6.7, and fermentation time is -9 days 6 days；And/or the second condition packet
Include: atomisation pressure 0.3-0.6MPa, charging rate 0.15-0.5m/s, hot blast temperature are 120-150 DEG C.
10. a kind of purposes of Moringa feed addictive as described in any one in claim 1-5, which is characterized in that the Moringa
Feed addictive is applied to aquatic feeds or the additive agent field of feed is supported in herding.
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