CN114271479A - Preparation method of fermented moringa oleifera rich in natural folic acid - Google Patents
Preparation method of fermented moringa oleifera rich in natural folic acid Download PDFInfo
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- CN114271479A CN114271479A CN202111664681.8A CN202111664681A CN114271479A CN 114271479 A CN114271479 A CN 114271479A CN 202111664681 A CN202111664681 A CN 202111664681A CN 114271479 A CN114271479 A CN 114271479A
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- Prior art keywords
- folic acid
- moringa oleifera
- leaf powder
- rich
- moringa
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- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 title claims abstract description 102
- 235000011347 Moringa oleifera Nutrition 0.000 title claims abstract description 62
- 235000019152 folic acid Nutrition 0.000 title claims abstract description 52
- 244000179886 Moringa oleifera Species 0.000 title claims abstract description 51
- 239000011724 folic acid Substances 0.000 title claims abstract description 51
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 title claims abstract description 50
- 229960000304 folic acid Drugs 0.000 title claims abstract description 50
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000000843 powder Substances 0.000 claims abstract description 41
- 238000000855 fermentation Methods 0.000 claims abstract description 24
- 230000004151 fermentation Effects 0.000 claims abstract description 24
- 239000000725 suspension Substances 0.000 claims abstract description 23
- 230000001580 bacterial effect Effects 0.000 claims abstract description 17
- 238000011081 inoculation Methods 0.000 claims abstract description 15
- 230000008014 freezing Effects 0.000 claims abstract description 12
- 238000007710 freezing Methods 0.000 claims abstract description 12
- 238000001694 spray drying Methods 0.000 claims abstract description 12
- 239000008223 sterile water Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 241000220215 Moringa Species 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 238000005516 engineering process Methods 0.000 claims abstract description 7
- 230000003213 activating effect Effects 0.000 claims abstract description 6
- 239000007858 starting material Substances 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 11
- 241000186840 Lactobacillus fermentum Species 0.000 claims description 8
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 8
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 8
- 229940012969 lactobacillus fermentum Drugs 0.000 claims description 8
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 8
- 235000010855 food raising agent Nutrition 0.000 claims description 5
- 241000186612 Lactobacillus sakei Species 0.000 claims description 4
- 241000235645 Pichia kudriavzevii Species 0.000 claims description 4
- 229940081969 saccharomyces cerevisiae Drugs 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 230000000813 microbial effect Effects 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000001502 supplementing effect Effects 0.000 abstract description 4
- 239000000126 substance Substances 0.000 description 8
- 235000013305 food Nutrition 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 4
- 238000009395 breeding Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 239000006041 probiotic Substances 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- 230000002787 reinforcement Effects 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000000433 anti-nutritional effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 229940014144 folate Drugs 0.000 description 1
- -1 folic acid Natural products 0.000 description 1
- 150000002224 folic acids Chemical class 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a preparation method of fermented moringa oleifera rich in natural folic acid, which comprises the following steps: s1, preparing inoculation liquid, namely, activating and expanding the fermentation strain, centrifuging and preparing bacteria suspension by using sterile water; s2, inoculating and culturing, namely adding sterile water into moringa oleifera leaf powder to serve as moringa oleifera leaf powder culture solution, inoculating the bacterial suspension serving as a biological starter, adjusting the initial pH value to 5.0-9.0, and culturing at the temperature of 30-45 ℃ for 12-96h to obtain moringa oleifera leaf powder fermentation liquor; s3, freezing and spray drying, and treating the moringa oleifera leaf powder fermentation liquor by using a freezing and spray drying technology to obtain the fermented moringa oleifera leaf powder rich in natural folic acid. The invention can strengthen the folic acid in the moringa leaves, so that the folic acid content is higher, and the effect of supplementing enough natural folic acid can be achieved by taking a very small amount of moringa.
Description
Technical Field
The invention relates to the technical field of food processing, in particular to a preparation method of fermented moringa oleifera rich in natural folic acid.
Background
Folic acid is essential nutrient for human body, the average intake of most residents is far from the recommended intake value, and the residents all have the need of increasing the intake of folic acid. Folic acid can be divided into synthetic and natural folic acids. The synthesis method of the Folic acid (Folic acid) has some environmental pollution, and excessive supplement of the Folic acid is potentially harmful to human health because the Folic acid is absorbed by the liver. Natural folic acid (Folate) is not harmful to human health due to its absorption in the intestinal tract. The moringa leaves are rich in folic acid and are good sources for supplementing natural folic acid and other nutrient substances.
There are many methods for strengthening folic acid in food, such as direct mixing method, physical method, chemical method, feeding enrichment, germination strengthening, genetic engineering technology, breeding method, and microbial fermentation technology. The direct mixing method uses synthetic folic acid, has potential hazard due to excessive supplement, and is contrary to the pursuit of natural health substances. Physical methods, folic acid fortification in different food matrices, remain to be verified. It is not clear whether chemical induction will produce adverse effects. Feeding enrichment is limited to poultry egg products. The method is only suitable for folic acid reinforcement of germinated foods such as bean sprouts. The genetic engineering technology and the breeding means solve the problem of folic acid reinforcement from food sources, have the defect of long research period and have low public acceptance of transgenic crops.
Disclosure of Invention
The invention aims to provide a preparation method of fermented moringa oleifera rich in natural folic acid, which can strengthen the folic acid in moringa oleifera leaves to ensure that the folic acid content is higher, and the effect of supplementing enough natural folic acid can be achieved by taking a very small amount of moringa oleifera.
The technical purpose of the invention is realized by the following technical scheme:
a preparation method of fermented moringa oleifera rich in natural folic acid comprises the following steps:
s1 preparation of inoculation liquid
Activating and expanding the fermentation strain, centrifuging, and preparing a bacterial suspension by using sterile water;
s2, inoculation and culture
Adding sterile water into moringa oleifera leaf powder to serve as culture solution of the moringa oleifera leaf powder, inoculating bacterial suspension serving as a biological fermentation agent, adjusting the initial pH value to be 5.0-9.0, and culturing at the temperature of 30-45 ℃ for 12-96h to obtain moringa oleifera leaf powder fermentation liquor;
s3, freezing spray drying
And (3) treating the moringa oleifera leaf powder fermentation liquor by using a freezing spray drying technology to obtain the fermented moringa oleifera leaf powder rich in natural folic acid.
Further preferably, in step S2, the bio-starter is a mixture of one or three bacterial suspensions selected from lactobacillus plantarum, lactobacillus fermentum, lactobacillus sake, saccharomyces cerevisiae, and pichia kudriavzevii.
Further preferably, in step S2, the biological fermentation agent is a mixture of three bacterial suspensions, i.e., lactobacillus plantarum, lactobacillus fermentum, and saccharomyces cerevisiae.
Further preferably, the volume ratio of the suspension of lactobacillus plantarum to the suspension of lactobacillus fermentum to the suspension of saccharomyces cerevisiae is 1:1: 1.
More preferably, in step S1, the bacterial suspension has a bacterial content of 108-109CFU/mL。
Preferably, in step S2, the amount of sterile water added to the moringa leaf powder is 10-20 times of the mass of the moringa leaf powder, and 1% -6% (v/v, in terms of the volume percentage of the moringa leaf powder culture solution) of the microbial starter is added to the moringa leaf powder culture solution.
More preferably, in step S2, the culture system after inoculation contains 106-107CFU/mL of fermentation broth.
Compared with the prior art, the invention has the following beneficial effects:
firstly, the probiotics selected by the invention have the capacity of producing vitamins such as folic acid, and the produced folic acid can be secreted into the moringa oleifera leaf powder, so that the folic acid content of the moringa oleifera leaf powder is obviously improved, the obtained natural folic acid is strengthened fermented moringa oleifera leaf powder, and the effect of supplementing enough natural folic acid when a very small amount of folic acid is ingested is achieved;
secondly, the probiotics can also degrade indigestible macromolecular nutrient substances such as protein and the like into easily absorbed micromolecular nutrient substances, simultaneously reduce the content of anti-nutritional factor substances in the moringa oleifera leaf powder, and improve the bioavailability of various nutrient substances;
thirdly, the defect of chemically synthesized folic acid is overcome, and meanwhile, the economic benefit is increased and the environmental pollution is reduced.
Drawings
FIG. 1 is a schematic flow chart of the operation of the present invention.
Detailed Description
In order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention is further described in detail with reference to the following examples. It will be appreciated by those skilled in the art that the following examples are illustrative of the invention only and should not be taken as limiting the scope of the invention.
Example 1
A preparation method of fermented moringa oleifera rich in natural folic acid comprises the following steps:
s1 preparation of inoculation liquid
Activating and expanding Lactobacillus plantarum, Lactobacillus fermentum and Saccharomyces cerevisiae, centrifuging, and making into bacterial suspension with bacteria content of 5 × 108CFU/mL；
S2, inoculation and culture
Adding 15 times of sterile water as a moringa oleifera leaf powder culture solution, inoculating 3% (v/v) of bacterial suspension of lactobacillus plantarum, lactobacillus fermentum and saccharomyces cerevisiae as a biological leavening agent according to the volume ratio of 1:1:1, wherein the inoculated culture system contains 5 multiplied by 106Adjusting the initial pH value of the CFU/mL fermentation strain to 6.0, and culturing at 30-45 deg.C for 50h to obtain Moringa oleifera leaf powder fermentation liquid;
s3, freezing spray drying
And (3) treating the moringa oleifera leaf powder fermentation liquor by using a freezing spray drying technology to obtain the fermented moringa oleifera leaf powder rich in natural folic acid.
Example 2
A preparation method of fermented moringa oleifera rich in natural folic acid comprises the following steps:
s1 preparation of inoculation liquid
Activating and enlarging culturing Lactobacillus sake, centrifuging, and making into bacterial suspension with bacteria content of 108CFU/mL；
S2, inoculation and culture
Adding sterile water with the mass of 20 times into the moringa oleifera leaf powder to serve as a moringa oleifera leaf powder culture solution, inoculating lactobacillus sake suspension serving as a biological leavening agent, wherein the inoculation amount is 6% (v/v) in percentage by volume of the moringa oleifera leaf powder culture solution, and a culture system after inoculation contains 10 percent6Adjusting the initial pH value of the fermentation strain to 7.0 by CFU/mL, and culturing at 30-45 deg.C for 12h to obtain Moringa oleifera leaf powder fermentation liquid;
s3, freezing spray drying
And (3) treating the moringa oleifera leaf powder fermentation liquor by using a freezing spray drying technology to obtain the fermented moringa oleifera leaf powder rich in natural folic acid.
Example 3
A preparation method of fermented moringa oleifera rich in natural folic acid comprises the following steps:
s1 preparation of inoculation liquid
Activating and expanding Pichia kudriavzevii, centrifuging, and making into bacterial suspension with bacteria content of 109CFU/mL；
S2, inoculation and culture
Adding 10 times of sterile water as a culture solution of moringa oleifera leaf powder, inoculating 1% (v/v) of a bacterial suspension of pichia kudriavzevii as a biological leavening agent, wherein the inoculated culture system contains 10 percent of sterile water7Adjusting the initial pH value of the CFU/mL fermentation strain to 5.0, and culturing at 30-45 deg.C for 96h to obtain Moringa oleifera leaf powder fermentation liquid;
s3, freezing spray drying
And (3) treating the moringa oleifera leaf powder fermentation liquor by using a freezing spray drying technology to obtain the fermented moringa oleifera leaf powder rich in natural folic acid.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (7)
1. A preparation method of fermented moringa oleifera rich in natural folic acid is characterized by comprising the following steps:
s1 preparation of inoculation liquid
Activating and expanding the fermentation strain, centrifuging, and preparing a bacterial suspension by using sterile water;
s2, inoculation and culture
Adding sterile water into moringa oleifera leaf powder to serve as culture solution of the moringa oleifera leaf powder, inoculating bacterial suspension serving as a biological fermentation agent, adjusting the initial pH value to be 5.0-9.0, and culturing at the temperature of 30-45 ℃ for 12-96h to obtain moringa oleifera leaf powder fermentation liquor;
s3, freezing spray drying
And (3) treating the moringa oleifera leaf powder fermentation liquor by using a freezing spray drying technology to obtain the fermented moringa oleifera leaf powder rich in natural folic acid.
2. The method for preparing the fermented moringa oleifera rich in natural folic acid according to the claim 1, is characterized in that: in step S2, the biological leavening agent is selected from one or more of Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus sake, Saccharomyces cerevisiae, and Pichia kudriavzevii.
3. The method for preparing the fermented moringa oleifera rich in natural folic acid according to the claim 2, characterized in that: in step S2, the biological leavening agent is a mixture of three bacterial suspensions of lactobacillus plantarum, lactobacillus fermentum and saccharomyces cerevisiae.
4. The method for preparing the fermented moringa oleifera rich in natural folic acid according to the claim 3, is characterized in that: the volume ratio of the suspension of the lactobacillus plantarum to the suspension of the lactobacillus fermentum to the suspension of the saccharomyces cerevisiae is 1:1: 1.
5. The method for preparing fermented moringa oleifera rich in natural folic acid according to any one of claims 1 to 4, characterized in that: in step S1, the bacterial suspension has a bacterial content of 108-109CFU/mL。
6. The method for preparing fermented moringa oleifera rich in natural folic acid according to any one of the claims 1, characterized in that: in the step S2, the amount of the sterile water added into the moringa leaf powder is 10-20 times of the mass of the moringa leaf powder, and 1-6% (v/v, calculated according to the volume percentage of the moringa leaf powder culture solution) of a microbial starter is inoculated into the moringa leaf powder culture solution.
7. The method for preparing fermented moringa oleifera rich in natural folic acid according to any one of the claims 1, characterized in that: in step S2, the culture system after inoculation contains 106-107CFU/mL of fermentation broth.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105105031A (en) * | 2015-09-23 | 2015-12-02 | 昆明理工大学 | Method for producing fermented soybean rich in folic acid |
| CN107811293A (en) * | 2016-09-13 | 2018-03-20 | 袁维理 | Moringa enzyme |
| CN109007295A (en) * | 2018-06-28 | 2018-12-18 | 中国热带农业科学院农产品加工研究所 | A kind of Moringa feed addictive, preparation method and purposes |
| CN110250270A (en) * | 2019-07-08 | 2019-09-20 | 北京工商大学 | A method of acidified milk folate content is improved using lactobacillus plantarum |
| JP2020150918A (en) * | 2019-03-22 | 2020-09-24 | 阿万 恵理 | Moringa fermented food |
-
2021
- 2021-12-31 CN CN202111664681.8A patent/CN114271479A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105105031A (en) * | 2015-09-23 | 2015-12-02 | 昆明理工大学 | Method for producing fermented soybean rich in folic acid |
| CN107811293A (en) * | 2016-09-13 | 2018-03-20 | 袁维理 | Moringa enzyme |
| CN109007295A (en) * | 2018-06-28 | 2018-12-18 | 中国热带农业科学院农产品加工研究所 | A kind of Moringa feed addictive, preparation method and purposes |
| JP2020150918A (en) * | 2019-03-22 | 2020-09-24 | 阿万 恵理 | Moringa fermented food |
| CN110250270A (en) * | 2019-07-08 | 2019-09-20 | 北京工商大学 | A method of acidified milk folate content is improved using lactobacillus plantarum |
Non-Patent Citations (1)
| Title |
|---|
| 张海燕;柳陈坚;何树芬;李晓然;: "高产叶酸植物乳杆菌的筛选及应用研究", 食品与生物技术学报, no. 05, pages 487 - 494 * |
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Application publication date: 20220405 |