CN109007370A - Complex function additive for feed for piglets and preparation method thereof - Google Patents
Complex function additive for feed for piglets and preparation method thereof Download PDFInfo
- Publication number
- CN109007370A CN109007370A CN201811002866.0A CN201811002866A CN109007370A CN 109007370 A CN109007370 A CN 109007370A CN 201811002866 A CN201811002866 A CN 201811002866A CN 109007370 A CN109007370 A CN 109007370A
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- CN
- China
- Prior art keywords
- feed
- complex function
- piglets
- rosa roxburghii
- roxburghii tratt
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Birds (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Sustainable Development (AREA)
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Abstract
The invention discloses a kind of complex function additive for feed for piglets and preparation method thereof, it is intended to solve the technical problem in Production of Livestock and Poultry.Complex function additive for feed for piglets is made of fresh Rosa roxburghii Tratt, Radix Astragali and tealeaves through lactobacillus plantarum and fermentation of bacillus subtilis；The mass fraction of fresh Rosa roxburghii Tratt, Radix Astragali and tealeaves matches are as follows: 50~80:20~30:10~15.Preparation method are as follows: select mature fresh Rosa roxburghii Tratt, remove Rosa roxburghii Tratt epidermis impurity, surface thorn and interior seed, break into fragment；By Radix Astragali and tealeaves impurity elimination, chopping；Activate lactobacillus plantarum and bacillus subtilis；Rosa roxburghii Tratt, Radix Astragali and tealeaves are mixed, by 1~2% inoculum concentration inoculated plant lactic acid bacteria and fermentation of bacillus subtilis；Tunning dry, pulverize, packing, sealing to get.Complex function additive for feed for piglets of the invention is green safe, can enhance human body immune function, improves resisting stress, anti-disease ability, improves production performance.
Description
Technical field
The present invention relates to field of feed additive technology, and in particular to a kind of complex function additive for feed for piglets and its system
Preparation Method.
Background technique
In scale Production of Livestock and Poultry, the life of domestic animal is influenced by various environmental factors, the variation meeting of environmental factor
Change the energy exchange between domestic animal and its environment, due to the influence of environment, weather, along with higher daily gain, faster
The speed of growth, cause stress phenomenon it is serious, and then immunity of organisms declines, and is easy the various diseases of infection.
Intracorporal antibody level is substantially reduced after weaned piglet, is enhanced the inhibition to cellular immunity, is contained in blood
Immunoglobulin quantity is reduced, and is reduced so as to cause disease resistance and immunity.Piglet coat and subcutaneous fat are few, heat-insulating capability
It wipes the sweat, can not resist cold, to cold vulnerable.After wean, piglet can not obtain lactose by breast milk to promote the production of lactic acid, and
Its own can not secrete enough hydrochloric acid again be kept low pH value, cause stomach inner pH value excessively high, can not secrete
Enough enzymes cause the digestion nutriment being unable to fully.These nutriments not digested completely can largely enter large intestine,
And the substrate for becoming suitable pathogenic microorganism effect herein leads to piglet so that pathogen be promoted largely to be bred
Diarrhea.
Currently, the method for use has to ensure that piglet produces: guaranteeing dry, warming, hygienic, ventilation, wound in piglet colony house
Good feeding environment is made, to reduce disease incidence, improves survival rate；After the various vaccines of piggy injection, expelling parasite is carried out, by inside and outside
Helminth is driven away clean；High-quality feed is fed, and by the addition such as suitable electrolyte, antibiotic and multidimensional rhzomorph in drinking-water；
Enzyme preparation and flavoring agent are applied in due course.It is reported that its early weaning can effectively be made up by adding enzyme preparation in weanling pig daily ration
When body in the various digestive ferments that lack, be remarkably reinforced so that it be made to digest the ability of nutriment in feed, reduce the hair of diarrhea
It is raw, it is ensured that the speed of growth keeps normal.Flavoring agent is added in weanling pig feed, foreign odor can be alleviated, stimulated simultaneously
The secretion of saliva, gastric juice and pancreatic juice enhances its appetite, so that feed intake be made to increase.
However, the above method is time-consuming, laborious, expection is had not yet been reached in application effect.In Disease monitor, diagnosis, prevents, puts out
Etc. it is backward in technique in links；Although carrying out large scale of pig farm this year has certain development, highdensity feeding manner causes pig
The adverse circumstances of house miniclimate are difficult to improve.In addition, the abuse of antibiotics of pig starter feed blindness and feed addictive are unable to ensure pig
The quality and quality of product.
It would therefore be highly desirable to a kind of livestock and poultry feedstuff green safe, production cost is low be researched and developed, to improve poultry immunity of organism
Power, oxidation resistance and anti-stress ability improve animal digestion absorbability, to promote to grow, improve production performance.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of complex function additive for feed for piglets and preparation method thereof.With
Phase reach improve piglet growth performance, immunity of organisms, oxidation resistance, anti-stress ability, animal digestion absorbability mesh
's.
In order to solve the above technical problems, The technical solution adopted by the invention is as follows:
A kind of complex function additive for feed for piglets is designed, by fresh Rosa roxburghii Tratt, Radix Astragali and tealeaves through lactobacillus plantarum and withered grass bud
Spore bacillus fermentation is made；Wherein, the matter of fresh Rosa roxburghii Tratt (just picking or the no disease and pests harm fruit through Preservation Treatment), Radix Astragali and tealeaves
Measure ratio are as follows: 50~80:20~30:10~15.
Above-mentioned fermentation raw material be based on long-term practical studies, and in conjunction with a large amount of raw material screening, composite test and get.
Wherein not only raw material sources are abundant for selected Rosa roxburghii Tratt, and it is rich in having Polysaccharides from Rosa roxburghii, flavonoids, amino acid and minerals etc.
Nutriment can provide required nutrient for piglet, improve efficiency of feed utilization.In addition Rosa roxburghii Tratt vitamin C also rich in,
Content reaches 3%, and Rosa roxburghii Tratt is made to have the function of resisting stress, improve body anti-oxidation function, and piglet can be delayed to turn group, high temperature, break
Milk etc. stress, improve pig production capacity flavone compound have adjust blood lipid, eliminate oxygen radical, it is anti-oxidant, antiviral,
The physiological activity such as antitumor, anti-aging；Superoxide dismutase in Rosa roxburghii Tratt is the main component for removing body free radical, is had
Significant anti-oxidation efficacy；The polysaccharide immunity substance mass-energy in Rosa roxburghii Tratt enhances piglet human body immune function simultaneously, improves anti-
Disease ability.
Selected Radix Astragali contains active constituent astragalus polyose, flavones etc., and it is non-that research finds that astragalus polyose can significantly increase piglet
Specific immune function and humoral immune function.The tea polyphenols isoreactivity substance in astragalus polyose joint tealeaves in Radix Astragali can increase
Strong piglet human body immune function and oxidation resistance improve animal resisting stress, anti-disease ability, improve weaned piglets, nothing
It remains, be not likely to produce drug resistance.
Preferably, the lactobacillus plantarum is preserved in Chinese microorganism strain collection, number BNCC187897.It plants
Object lactobacillus ferment raw material, can produce a large amount of lactic acid, can improve feed palatability, improve alimentary canal beneficial to bacterial content.
Preferably, the bacillus subtilis is preserved in Chinese microorganism strain collection, number BNCC188080.
Bacillus subtilis consumes rapidly the free oxygen in enteron aisle, causes enteron aisle hypoxemia, promotes to grow beneficial to anaerobic bacteria, bacillus subtilis
Subtilin, polymyxins, the gramicidins isoreactivity substance generated in bacterium growth course, can inhibit pathogenic bacteria to grow.Withered grass
Bacillus thallus itself synthesizes the enzymes such as alpha-amylase, protease, lipase, cellulase, and feed digestibility can be improved.
The tunning obtained by lactobacillus plantarum and bacillus subtilis cooperation fermentation Rosa roxburghii Tratt, Radix Astragali and tealeaves, Neng Gougai
Kind mouthfeel, meets the needs of piglet is to organic acid and particular matter, maintains the balance and stabilization of organismic internal environment, and reduce digestion
Road pH value, protection gastrointestinal health activate proenzyme in body, promote feed digestion and absorption, and then guarantee body allomeric function
It gives full play to, diarrhea of weaned piglets can be effectively reduced, piglet health is kept, to play pig maximum growth performance.
Design the preparation method of complex function additive for feed for piglets described in one kind, comprising the following steps:
(1) mature fresh Rosa roxburghii Tratt is selected, Rosa roxburghii Tratt epidermis impurity, surface thorn and interior seed are removed；It will treated that Rosa roxburghii Tratt breaks into is small
Fragment；
(2) by Radix Astragali and tealeaves impurity elimination, chopping；
(3) activation culture lactobacillus plantarum and bacillus subtilis；
(4) by treated, Rosa roxburghii Tratt, Radix Astragali and tealeaves are uniformly mixed according to the above ratio as raw material, by total mass of raw material 1~2%
Inoculum concentration inoculated plant lactic acid bacteria and bacillus subtilis, anaerobic fermentation 48h under the conditions of 28~30 DEG C of temperature；
(5) tunning is dried, crush, packing, sealing to get.
Preferably, in the step (3), the culture medium prescription of the activation culture lactobacillus plantarum use are as follows: albumen
10.0 g of peptone, 5.0 g of beef extract, 4.0 g of yeast powder, 20.0 g of glucose, 1.0 ml of Tween 80,2.0 g of dipotassium hydrogen phosphate,
5.0 g of sodium acetate, 2.0 g of Triammonium citrate, 0.2 g of magnesium sulfate, 0.05 g of manganese sulfate, 15.0 g of agar are constituted, and 0.5L is added
Distilled water adjusts pH to 6.2, adds distilled water to 1.0L.
Preferably, in the step (3), the culture medium raw material formula of the activation culture gemma hay bacillus use
Are as follows: NaCl 5.0g, beef extract 10.0g, peptone 10.0g, agar 20.0g adjust pH to 7, add distilled water to 1.0L.
Further, the culture medium need to sterilize 30 minutes under 105 DEG C of condition of high voltage.
Preferably, in the step (4), when the inoculation, the ratio of lactobacillus plantarum and gemma hay bacillus is 8:
2。
Preferably, in the step (5), the mesh number of the crushing is 80~120 mesh.
Compared with prior art, the beneficial technical effect of the present invention lies in:
1. complex function additive for feed for piglets of the invention is by lactic acid bacteria and fermentation of bacillus subtilis Rosa roxburghii Tratt, Radix Astragali and tealeaves
Composite fermentation is made, vitamin C, flavonoids, Polyphenols, amino acid, microelement, tea polyphenols and vitamin rich in etc.
Active component can enhance human body immune function, improve resisting stress, anti-disease ability, improve production performance.
2. not containing any antibiotic ingredient in complex function additive for feed for piglets of the invention, noresidue is not easy to produce
Raw drug resistance, it is green safe, and each material combination degree of having, it complements each other: where the stomach invigorating of Rosa roxburghii Tratt tool, digestion gathered food is glutted, nourishing is strong
The effect of strong；Radix Astragali has tonifying middle-Jiao and Qi, hidroschesis, inducing diuresis for removing edema, removes the effect of taking poison and growing muscle due；The tranquilizing the mind of tealeaves tool, clearing heat and detoxicating, wind-dispelling
Inducing diaphoresis, help digestion, benefiting energy the effect of；Fermentation can not only remove acerbity, the substance that tarts up, the mouthfeel that reconciles, moreover it is possible to which degradation is big
Molecular substance ingredient, more conducively piglet are digested and assimilated.
3. complex function additive for feed for piglets of the invention is green safe, at low cost, it is easy to apply and promotes.
Specific embodiment
Illustrate a specific embodiment of the invention below with reference to embodiment, but following embodiment is used only to be described in detail
The present invention does not limit the scope of the invention in any way.
Related instrument and equipment is routine instrument device unless otherwise instructed in the examples below；It is related
Reagent is commercially available conventional reagent unless otherwise instructed；Related test method is unless otherwise instructed conventional method.
A kind of embodiment one: complex function additive for feed for piglets
1. complex function additive for feed for piglets raw material proportioning are as follows:
In terms of quality proportioning, 50 parts of fresh Rosa roxburghii Tratt, 30 parts of Radix Astragali, 15 parts of tealeaves.
2. complex function additive for feed for piglets the preparation method comprises the following steps:
(1) it prepares Rosa roxburghii Tratt: selecting mature fresh Rosa roxburghii Tratt, remove Rosa roxburghii Tratt epidermis impurity, surface thorn and interior seed；Will treated thorn
Pears break into fine grained chippings；
(2) by Radix Astragali and tealeaves impurity elimination, chopping；
(3) lactobacillus plantarum (being purchased from Chinese microorganism strain collection, number BNCC187897) activation culture；
Lactobacillus plantarum freeze-dried powder activation medium is by 10.0 g of peptone, 5.0 g of beef extract, 4.0 g of yeast powder, glucose
20.0 g, 1.0 ml of Tween 80,2.0 g of dipotassium hydrogen phosphate, 5.0 g of sodium acetate, 2.0 g of Triammonium citrate, 0.2 g of magnesium sulfate,
0.05 g of manganese sulfate, 15.0 g of agar are constituted, and 0.5L distilled water is added and is finally prepared with distilled water with dilute hydrochloric acid tune pH to 6.2
At 1.0L.105 DEG C high pressure sterilization 30 minutes.
(4) bacillus subtilis (being purchased from Chinese microorganism strain collection, number BNCC188080) activation culture；
Gemma hay bacillus culture medium, NaCl 5.0g, beef extract 10.0g, peptone 10.0g, agar 20.0g, distilled water
1.0L, adjust pH to 7,105 DEG C high pressure sterilization 30 minutes.
(5) by treated, Rosa roxburghii Tratt, Radix Astragali and tealeaves are added in sealed fermenting tank after mixing as raw material, by original
1% inoculum concentration inoculated plant lactic acid bacteria of gross mass and bacillus subtilis are expected into raw material, lactobacillus plantarum and gemma when inoculation
The ratio of hay bacillus is 8:2；Anaerobic fermentation 48h under the conditions of 28 DEG C of temperature.
(6) after entire tunning being dried, 80 mesh are crushed to, are dispensed, are sealed to get complex function pig starter feed
Additive.
A kind of embodiment two: complex function additive for feed for piglets
With embodiment one the difference is that:
1. complex function additive for feed for piglets raw material proportioning are as follows:
In terms of quality proportioning, 70 parts of fresh Rosa roxburghii Tratt, 25 parts of Radix Astragali, 10 parts of tealeaves.
2. complex function additive for feed for piglets the preparation method comprises the following steps:
(1) it prepares Rosa roxburghii Tratt: selecting mature fresh Rosa roxburghii Tratt, remove Rosa roxburghii Tratt epidermis impurity, surface thorn and interior seed；Will treated thorn
Pears break into fine grained chippings；
(2) by Radix Astragali and tealeaves impurity elimination, chopping；
(3) lactobacillus plantarum and bacillus subtilis activation culture；
(4) by treated, Rosa roxburghii Tratt, Radix Astragali and tealeaves are added in sealed fermenting tank after mixing as raw material, total by raw material
1.5% inoculum concentration inoculated plant lactic acid bacteria of quality and bacillus subtilis are into raw material, and lactobacillus plantarum and gemma are withered when inoculation
The ratio of straw bacterium is 8:2；Anaerobic fermentation 48h under the conditions of 29 DEG C of temperature.
(5) after entire tunning being dried, 100 mesh are crushed to, are dispensed, sealing is to get the feeding of complex function piglet
Feed additives.
A kind of embodiment three: complex function additive for feed for piglets
With embodiment one the difference is that:
1. complex function additive for feed for piglets raw material proportioning are as follows:
In terms of quality proportioning, 80 parts of fresh Rosa roxburghii Tratt, 20 parts of Radix Astragali, 12 parts of tealeaves.
2. complex function additive for feed for piglets the preparation method comprises the following steps:
(1) it prepares Rosa roxburghii Tratt: selecting mature fresh Rosa roxburghii Tratt, remove Rosa roxburghii Tratt epidermis impurity, surface thorn and interior seed；Will treated thorn
Pears break into fine grained chippings；
(2) by Radix Astragali and tealeaves impurity elimination, chopping；
(3) lactobacillus plantarum and bacillus subtilis activation culture；
(4) by treated, Rosa roxburghii Tratt, Radix Astragali and tealeaves are added in sealed fermenting tank after mixing as raw material, total by raw material
2% inoculum concentration inoculated plant lactic acid bacteria of quality and bacillus subtilis are into raw material, lactobacillus plantarum and gemma withered grass when inoculation
The ratio of bacillus is 8:2；Anaerobic fermentation 48h under the conditions of 30 DEG C of temperature.
(5) after entire tunning being dried, 120 mesh are crushed to, are dispensed, sealing is to get the feeding of complex function piglet
Feed additives.
Test example: by the animal experiment of complex function additive for feed for piglets
1. test material
The complex function additive for feed for piglets of embodiment one.
2. experimental animal and experimental design
It is designed using single factor experiment, DLY weanling pig 50 for selecting 7kg or so are randomly divided into control group and test group.Often
5 repetitions of group, 5 pigs of each repetition.
Control group fed corn bean pulp type weanling pig daily ration, basal diet is referring to NRC(2012) 7~11kg piglet nutrition
Standard preparation, control group and test group basal diet proportion it is identical, be respectively corn 67.1%, dregs of beans 27.5%, soya-bean oil 1.6%,
Mountain flour 1%, calcium monohydrogen phosphate 0.9%, diatomite 1%, salt 0.5%, compound vitamin 0.13%, microelement pre-mix material 0.12%, L- rely
Propylhomoserin hydrochloride 0.35%, DL- METHIONINE 0.23%, L-threonine 0.15%, L-Trp 0.02%.Test group is in basal diet
Add the complex function additive for feed for piglets of the embodiment 1 of 500mg/kg.
Experimental period 28 days.It is young to wean after the complex function additive for feed for piglets that embodiment 1 is added in daily ration to investigate
Pig pig production performance, anti-stress ability, anti-oxidation function and the influence to intestine of young pigs health.
3. testing index and method
(1) production performance index
The daily feed intake of each pigsty during accurate recording is tested, distinguishes before on-test and after the test as unit of circle
It weighs on an empty stomach, calculates every pig average daily gain, average daily gain and feed conversion rate.
(2) diarrhea rate
Every morning 8:00 point timing observation piglet excrement situation.Diarrhea head number is recorded, diarrhea rate is calculated.
Diarrhea rate (%)=total diarrhea number/(total number of elements × test number of days) × 100%；
(3) Serum antioxidant indices measure
Before test 28d, 8 a.m. empty stomach, each circle respectively selects 1 piglet, 10 mL of jugular vein blood collection after weighing, stands
After 4h, 3000 r/ min be centrifuged 15 min, in absorption serum solution to survey Antioxidant Indexes GSH-Px, SOD and MDA in serum,
Kit measurement is built up using Nanjing, specific measuring method is referring to kit specification.
(4) microculture and counting
It tests the 25th day, each circle randomly selects 1 sodium selenite, sterilizes anus with cotton ball soaked in alcohol, fresh excrement sample is taken to put immediately
Enter in sterilized plastic bag, Bacillus acidi lactici selective medium and Mai Kangkai culture medium is respectively adopted, cultivate and calculates fresh excrement
Middle Escherichia coli and Bacillus acidi lactici number.Microflora is indicated with the fresh excrement sample of Log10N/g.
4. test result
(1) to the influence of pig growth performance
Influence of the complex function additive for feed for piglets to pig growth performance is as shown in table 1.
Influence of the 1 feeding feed stuff additive of table to Growth Performance of Weaning Piglets
。
Note: after data different letters respectively indicate with significant difference between annual same column different disposal (P< 0.05), similarly hereinafter.
The complex function additive for feed for piglets that table 1 gives weanling pig supplementary feeding of the invention as the result is shown, can make test pig
Daily gain improves 16.30%(than control groupP< 0.05), feed intake improves 4.22%, and feed-weight ratio reduces 10.34%(P< 0.05), table
Bright swine rations add the additive and are remarkably improved production performance.
(2) to the influence of antioxidant ability of organism
Influence of the complex function additive for feed for piglets to antioxidant ability of organism is as shown in table 2.
Influence of the 2 feeding feed stuff additive of table to Growth Performance of Weaning Piglets
。
Table 2 is remarkably improved blood the result shows that the complex function additive for feed for piglets for giving weanling pig supplementary feeding of the invention
GSH-Px and SOD activity in clear (P< 0.05), significantly reduce MDA content (P< 0.05) it is anti-oxidant, that is, to significantly improve body
Ability.
(3) to the influence of microbial flora in grice diarrhoea and excrement
Influence to weanling pig supplementary feeding complex function additive for feed for piglets to microbial flora in grice diarrhoea and excrement
As shown in table 3.
Influence of the 3 feeding feed stuff additive of table to micro organism quantity in diarrhea of weaned piglets and excrement
。
Table 3 the results show that compared with control group, test group significantly reduce piglet diarrhea rate (P< 0.05), it is big in excrement
Enterobacteria quantity without significant difference (P> 0.05) but Bacillus acidi lactici quantity dramatically increase (P< 0.05), show that the additive is adjustable
Section intestine of young pigs flora reduces grice diarrhoea in turn.
The present invention is described in detail above in conjunction with embodiment, still, person of ordinary skill in the field can
Understand, without departing from the purpose of the present invention, each design parameter in above-described embodiment can also be changed, shape
At multiple specific embodiments, it is common variation range of the invention, is no longer described in detail one by one herein.
Claims (9)
1. a kind of complex function additive for feed for piglets, which is characterized in that by fresh Rosa roxburghii Tratt, Radix Astragali and tealeaves through lactobacillus plantarum
It is made with fermentation of bacillus subtilis；Wherein, the mass ratio of fresh Rosa roxburghii Tratt, Radix Astragali and tealeaves are as follows: 50~80:20~30:10~
15。
2. complex function additive for feed for piglets according to claim 1, which is characterized in that the lactobacillus plantarum is to protect
It is hidden in the BNCC187897 of Chinese microorganism strain collection.
3. complex function additive for feed for piglets according to claim 1, which is characterized in that the bacillus subtilis is
It is preserved in the BNCC188080 of Chinese microorganism strain collection.
4. a kind of preparation method of complex function additive for feed for piglets described in claim 1, which is characterized in that including following
Step:
(1) mature fresh Rosa roxburghii Tratt is selected, Rosa roxburghii Tratt epidermis impurity, surface thorn and interior seed are removed；It will treated that Rosa roxburghii Tratt breaks into is small
Fragment；
(2) by Radix Astragali and tealeaves impurity elimination, chopping；
(3) activation culture lactobacillus plantarum and bacillus subtilis；
(4) by treated, Rosa roxburghii Tratt, Radix Astragali and tealeaves are uniformly mixed as raw material in ratio described in claim 1, total by raw material
1~2% inoculum concentration inoculated plant lactic acid bacteria of quality and bacillus subtilis, anaerobic fermentation 48h under the conditions of 28~30 DEG C of temperature；
(5) tunning is dried, crush, packing, sealing to get.
5. the preparation method of complex function additive for feed for piglets according to claim 4, which is characterized in that in the step
Suddenly in (3), the culture medium raw material formula of the activation culture lactobacillus plantarum use are as follows: 10.0 g of peptone, beef extract 5.0
G, 4.0 g of yeast powder, 20.0 g of glucose, 1.0 ml of Tween 80,2.0 g of dipotassium hydrogen phosphate, 5.0 g of sodium acetate, citric acid three
2.0 g of ammonium, 0.2 g of magnesium sulfate, 0.05 g of manganese sulfate, 15.0 g of agar are constituted, and 0.5L distilled water is added, and are adjusted pH to 6.2, are added steaming
Distilled water is to 1.0L.
6. the preparation method of complex function additive for feed for piglets according to claim 4, which is characterized in that in the step
Suddenly in (3), the culture medium prescription of the activation culture gemma hay bacillus use are as follows: NaCl 5.0g, beef extract 10.0g, egg
White peptone 10.0g, agar 20.0g adjust pH to 7, add distilled water to 1.0L.
7. the preparation method of complex function additive for feed for piglets according to claim 5 or 6, which is characterized in that described
Culture medium need to sterilize 30 minutes under 105 DEG C of condition of high voltage.
8. the preparation method of complex function additive for feed for piglets according to claim 4, which is characterized in that in the step
Suddenly in (4), when the inoculation, the ratio of lactobacillus plantarum and gemma hay bacillus is 8:2.
9. the preparation method of complex function additive for feed for piglets according to claim 4, which is characterized in that in the step
Suddenly in (5), the mesh number of the crushing is 80~120 mesh.
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| CN113068771A (en) * | 2021-03-29 | 2021-07-06 | 贵州省畜牧兽医研究所 | Formula, preparation method and application of feed additive |
| CN113331311A (en) * | 2021-06-11 | 2021-09-03 | 山东玖瑞农业集团有限公司 | Lactobacillus plantarum and application thereof in pig feed |
| CN113331311B (en) * | 2021-06-11 | 2022-12-09 | 山东玖瑞农业集团有限公司 | Lactobacillus plantarum and application thereof in pig feed |
| CN113907208A (en) * | 2021-11-04 | 2022-01-11 | 淮阴工学院 | Feed additive for preventing piglet diarrhea and preparation method and application thereof |
| CN113907208B (en) * | 2021-11-04 | 2024-02-23 | 淮阴工学院 | Feed additive for preventing diarrhea of piglets, and preparation method and application thereof |
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