CN102106487A - Biological fermentation feed for laying chickens and production method - Google Patents
Biological fermentation feed for laying chickens and production method Download PDFInfo
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- CN102106487A CN102106487A CN2011100344034A CN201110034403A CN102106487A CN 102106487 A CN102106487 A CN 102106487A CN 2011100344034 A CN2011100344034 A CN 2011100344034A CN 201110034403 A CN201110034403 A CN 201110034403A CN 102106487 A CN102106487 A CN 102106487A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a biological fermentation feed for laying chickens and a production method. The feed can improve the digestion, absorption and utilization rate of the laying chickens on nutrients in a vegetable feed, improve the production capacity of the laying chickens and improve the quality of eggs, and is used in a compound feed for the laying chickens. Bean pulp, rice bran and wheat bran are used as raw materials; the feed is formed by fermentation of liquid strains in an amount which is 32 percent of the mass of the raw materials and solid strains in an amount which is 10 percent of the mass of the raw materials; the mass ratio of the bean pulp, the rice bran to the wheat bran is 70: 20: 10; the liquid strains consist of saccharomycetes, bacillus and alcaligenes in a volume ratio of 70: 20: 10, and the activity unit of each strain is 10<10> to 10<14>cfu/ml; and the solid strains consist of aspergillus niger, aspergillus oryzae and rhizopus in a mass ratio of 60: 30: 30, and the activity unit of each strain is 10<10> to 10<14>cfu/g.
Description
Technical field
The present invention relates to a kind of feedstuff for laying hens and production method, especially a kind of improve laying hen to the rate that digests and absorbs of nutriment in the plant feed, improve laying hen production capacity, improve egg quality, the laying hen of using in laying-hen compound feed is with biological fermentation feed and production method.
Background technology
At present, existing about in broiler fodder, adding the report of probio, as the patent No. is that 200410068185.6 application for a patent for invention discloses " a kind of feed level microbe additive and preparation thereof with use ", is partly to form with the nutrient solution that is made of butyric acid, acetate, propionic acid is partially mixed by biting the probio that Lactobacillus lactis, enterococcus faecalis, clostridium butyricum constitute.That the patent No. is that 200710158252.7 patent of invention discloses is a kind of " FQ15 enterococcus faecalis and adopt this bacterial classification to produce the method for feed additive for promoting growth ".More than two patents all be that beneficial bacterium is directly added in the feed as additive, its purpose all is in order to impel the fryer secretion and produce various nutriments in the fryer body, improve growth of meat chicken speed, increasing broiler weight.Described in patent specification, " in feed, add microbial forage additives such as probio; on the one hand; can secrete and produce nutriment (as B family vitamin 1,2,6,12; folic acid, pantothenic acid, VH and several amino acids and mycoprotein etc.); the balanced feed trophic structure, help improving efficiency of feed utilization.On the other hand, can secrete and produce lipase, protease and amylase, replenish the deficiency of enteron aisle digestive ferment, simultaneously, can also produce the enzyme that alimentary canals such as pectase, cellulase and dextranase do not have, nutriment digests and assimilates in the promotion enteron aisle.The more important thing is that microbial forage additive can increase and promote the growth of enteron aisle beneficial microorganism, breeding, intestines wall epithelial cell is formed effective stimulus, promote the growth of intestinal villus, make that intestines wall gauffer increases, lacuna deepens, thereby increased animal intestinal to the nutriment effective absorbing area, improved the absorbability of body nutriment; Probio can also produce somatomedin, has improved cell alkaline phosphatase (AKP) isoreactivity level, promotes the synthetic level of cell RNA, DNA and protein, has promoted growth of animal and weightening finish ".
Yet, be not purpose up to now with production capacity and the egg quality that improves laying hen, be exclusively used in the fermentative feedstuff of microbe of laying hen, to such an extent as to the laying hen annual egg has only 15kg, and go into to give up death rate more than 20%.
Summary of the invention
The present invention is in order to solve the above-mentioned technical problem of existing in prior technology, provide a kind of improve laying hen to the rate that digests and absorbs of nutriment in the plant feed, improve laying hen production capacity, improve egg quality, the laying hen of using in laying-hen compound feed is with biological fermentation feed and production method.
Technical solution of the present invention is: a kind of laying hen is used biological fermentation feed, it is characterized in that being is raw material with dregs of beans, rice bran and wheat bran, add the liquid spawn of raw material quality 32% and the solid spawn fermentation of raw material quality 10% and form, the mass ratio of described dregs of beans, rice bran and wheat bran is 70:20:10; Described liquid spawn is made up of saccharomycete, bacillus and Alcaligenes, and the volume ratio of saccharomycete, bacillus and Alcaligenes is 70:20:10, and the unit of activity of each bacterial classification is 10
10~ 10
14Cfu/ml; Described solid spawn is made up of black-koji mould, aspergillus oryzae and head mold, and the mass ratio of black-koji mould, aspergillus oryzae and head mold is 60:30:30, and the unit of activity of each bacterial classification is 10
10~ 10
14Cfu/g.
A kind of above-mentioned laying hen is used the production method of biological fermentation feed, it is characterized in that carrying out as follows successively:
A. getting dregs of beans, rice bran and wheat bran is raw material, and the mass ratio of dregs of beans, rice bran and wheat bran is 70:20:10, and raw material is sterilized;
B. the liquid spawn of adding raw material quality 32% and the solid spawn and the Shui Houpu of raw material quality 10% put solid state fermentation in the installation for fermenting in the raw material after sterilization, it is 20 ~ 50cm that thickness is put in the shop, 25 ~ 50 ℃ of fermentation temperatures, fermentation time is 48 ~ 96 hours, described liquid spawn is made up of saccharomycete, bacillus and Alcaligenes, the volume ratio of saccharomycete, bacillus and Alcaligenes is 70:20:10, and the unit of activity of each bacterial classification is 10
10~ 10
14Cfu/ml; Described solid spawn is made up of black-koji mould, aspergillus oryzae and head mold, and the mass ratio of black-koji mould, aspergillus oryzae and head mold is 60:30:30, and the unit of activity of each bacterial classification is 10
10~ 10
14Cfu/g;
C. after fermentation is finished material is carried out drying and pulverize getting final product, the dry materials temperature is not higher than 50 ℃.
Described liquid spawn is to cultivate as follows to form:
1.. scrape lawn or the spore of getting cultivation with oese, inserting 500ml under aseptic condition is equipped with in the triangular flask of the sterilized first order seed culture medium of 250ml, shaking table shaken cultivation 24 ~ 28h under 37 ℃ of conditions, promptly get primary seed solution, described first order seed culture medium is by beef extract 5g, peptone 10g, NaCl 5g, K2HPO
4The liquid of pH7.0 ~ 7.3 that 1g and water 1000ml constitute;
2.. the primary seed solution that obtains is linked into 2500ml by 10% inoculum concentration is equipped with in the triangular flask of the sterilized secondary seed medium of 1000ml, 37 ℃ of shaking table shaken cultivation 48h, described secondary seed medium is made of beef extract, peptone, brown sugar or molasses, NaCl and water, each raw material mass ratio is a beef extract 0.01, peptone 0.2, brown sugar or molasses 15, NaCl0.2, water 100.
Described solid spawn is to cultivate as follows to form:
1.. scrape lawn or the spore of getting cultivation with oese, inserting 500ml under aseptic condition is equipped with in the triangular flask of the sterilized first order seed culture medium of 250ml, shaking table shaken cultivation 24 ~ 28h under 37 ℃ of conditions, promptly get primary seed solution, described first order seed culture medium is made of potato 200g, sucrose 20g and water 1000ml, be potato to be smashed add that poach is ripe, mix with sucrose and water after the filtered through gauze, autoclaving again;
2.. with dregs of beans, KH
2PO
4, MgSO
4And water mixes and sterilization, promptly makes the secondary seed solid medium, dregs of beans, KH
2PO
4, MgSO
4And the mass ratio of water is 100:0.2:0.05:50 ~ 60;
3.. the secondary seed solid medium of sterilization is transferred to sort of quyi chamber, and according to stirring after the 10% inoculum concentration access primary seed solution, keeping water content is 60%, and the generation spore that continuously ferments in bamboo basket gets final product.
The present invention is that employing dregs of beans, rice bran and wheat bran are raw material, adds the bacterium liquid of saccharomycete, bacillus and Alcaligenes composition and the solid spawn of being made up of black-koji mould, aspergillus oryzae and head mold, adopts process for solid state fermentation production.The bacterium of the existing cellulose-decomposing bacteria of many bacterial classifications that the present invention adopts, nitrogen transformation bacterium, palatability also has the detoxification bacterial strain, compatible between each bacterial classification, synergy, and effect is more remarkable, more stable than single culture.Particularly vegetable protein raw materials such as dregs of beans, rice bran and wheat bran can produce a large amount of mycoproteins afterwards by fermentation, and protein content is all increased before the fermentation (improving 2 ~ 3%), but also have improved the quality of material protein.Be present in most of ANFs in the raw material through fermentation and destroyed, the mycotoxin that exists in the part material is eliminated through fermentation, also produce a large amount of little peptides, fodder enzyme preparation, B family vitamin, beneficial bacterium culture, UGF in the sweat simultaneously, improved the rate that digests and absorbs of vegetable protein raw material.Use the product of the present invention laying hen of feeding, can significantly improve laying hen production capacity, feedstuff-egg ratio reduces by 1.40%, can reduce simultaneously illness rates such as laying hen diarrhoea, reduce and go into to give up death rate 27.6%, both can reduce or replace antibiotic use, improved egg quality, avoid antibiotic to animal and side effect that human body brought, finally improve the economic benefit of breeding layer chicken.The present invention adopts solid state fermentation, need not bacterial classification is separated, and equipment such as promptly need not to separate, concentrate, and also can not produce a large amount of organic wastewaters of handling of needing, and has not only reduced production cost, and has been beneficial to environmental protection.
The specific embodiment
Laying hen of the present invention is used the production method of biological fermentation feed, carries out as follows successively:
A. getting existing vegetable protein feed-dregs of beans, rice bran and wheat bran is raw material, 10 kilograms in 70 kilograms of dregs of beans, 20 kilograms in rice bran and wheat, raw material packed into sterilize in the special-purpose bactericidal unit (rotatable, turn over throwing), sterilising conditions: 121 ℃ of temperature, time: 30min;
B. after adding the solid spawn and water of 32 kilograms liquid spawn and 10 kilograms in the raw material after sterilization, solid state fermentation in the installation for fermenting is put in the shop, it is 20 ~ 50cm that thickness is put in the shop, 25 ~ 50 ℃ of fermentation temperatures, fermentation time is 48 ~ 96 hours, described liquid spawn is made up of FGX-01 saccharomycete, HD bacillus and Alcaligenes XN01, and the volume ratio of saccharomycete, bacillus and Alcaligenes is 70:20:10, and the unit of activity of each bacterial classification is 10
10~ 10
14Cfu/ml; Described solid spawn is made up of black-koji mould HS03, aspergillus oryzae MQM002 and head mold GM001, and the mass ratio of black-koji mould, aspergillus oryzae and head mold is 60:30:30, and the unit of activity of each bacterial classification is 10
10~ 10
14Cfu/g;
Each bacterial classification is all purchased in Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology.
Described liquid spawn is to enlarge as follows to cultivate to form:
1.. scrape lawn or the spore of getting cultivation with oese, inserting 500ml under aseptic condition is equipped with in the triangular flask of the sterilized first order seed culture medium of 250ml, shaking table shaken cultivation 24 ~ 28h under 37 ℃ of conditions, promptly get primary seed solution, described first order seed culture medium is by beef extract 5g, peptone 10g, NaCl 5g, K2HPO
4The liquid of pH7.0 ~ 7.3 that 1g and water 1000ml constitute;
2.. the primary seed solution that obtains is linked into 2500ml by 10% inoculum concentration is equipped with in the triangular flask of the sterilized secondary seed medium of 1000ml, 37 ℃ of shaking table shaken cultivation 48h, described secondary seed medium is made of beef extract, peptone, brown sugar or molasses, NaCl and water, each raw material mass ratio is a beef extract 0.01, peptone 0.2, brown sugar or molasses 15, NaCl0.2, water 100.
Described solid spawn is to enlarge as follows to cultivate to form:
1.. scrape lawn or the spore of getting cultivation with oese, inserting 500ml under aseptic condition is equipped with in the triangular flask of the sterilized first order seed culture medium of 250ml, shaking table shaken cultivation 24 ~ 28h under 37 ℃ of conditions, promptly get primary seed solution, described first order seed culture medium is made of potato 200g, sucrose 20g and water 1000ml, be potato to be smashed add that poach is ripe, mix with sucrose and water after the filtered through gauze, autoclaving again;
2.. with dregs of beans, KH
2PO
4, MgSO
4And water mixes and sterilization, promptly makes the secondary seed solid medium, dregs of beans, KH
2PO
4(feed grade), MgSO
4The mass ratio of (feed grade) and water is 100:0.2:0.05:50 ~ 60;
3.. the secondary seed solid medium of sterilization is transferred to sort of quyi chamber, and according to stirring after the 10% inoculum concentration access primary seed solution, keeping water content is 60%, and the generation spore that continuously ferments in bamboo basket gets final product.
The fermentation water adopts chlorine dioxide to sterilize, and concentration is 20ppm.
After fermentation is finished material is carried out drying and pulverize getting final product, the dry materials temperature is not higher than 50 ℃, and product is a powder, and specification is all by the 2.8mm wire gauze sieve.
Employing is diluted to flat band method and detects, and the colony counts in the feed that embodiment of the invention gained ferments can reach 10
9~ 10
11Cfu/g.
This product is through the breeding layer chicken test of the Co., Ltd of raising chickens at Dalian Han Wei, and test group is to add embodiment of the invention product in laying-hen compound feed, and addition is 3% ~ 5%, and control group is the mixed feed of all feeding.
Test group and control group contrast are as follows:
Test group laying hen feedstuff-egg ratio reduces by 1.4% than control group, the eggshell color improves 19.8% than control group, reduce broken soft egg rate and reduce by 9.00% than control group, shell thickness improves 2.47% than control group, and dirty egg ratio has descended 54.4% than control group, and arenaceous shell egg group has descended 48.41% than control group, deformity egg ratio has descended 77.04% than control group, the spot egg has descended 43.26% than control group, and dejecta moisture has reduced by 0.84% than control group, goes into to give up the chicken death rate and has reduced by 27.6% than control group.
Claims (4)
1. a laying hen is used biological fermentation feed, it is characterized in that being is raw material with dregs of beans, rice bran and wheat bran, add the liquid spawn of raw material quality 32% and the solid spawn fermentation of raw material quality 10% and form, the mass ratio of described dregs of beans, rice bran and wheat bran is 70:20:10; Described liquid spawn is made up of saccharomycete, bacillus and Alcaligenes, and the volume ratio of saccharomycete, bacillus and Alcaligenes is 70:20:10, and the unit of activity of each bacterial classification is 10
10~ 10
14Cfu/ml; Described solid spawn is made up of black-koji mould, aspergillus oryzae and head mold, and the mass ratio of black-koji mould, aspergillus oryzae and head mold is 60:30:30, and the unit of activity of each bacterial classification is 10
10~ 10
14Cfu/g.
One kind according to claim 1 laying hen it is characterized in that carrying out as follows successively with the production method of biological fermentation feed:
A. getting dregs of beans, rice bran and wheat bran is raw material, and the mass ratio of dregs of beans, rice bran and wheat bran is 70:20:10, and raw material is sterilized;
B. the liquid spawn of adding raw material quality 32% and the solid spawn and the Shui Houpu of raw material quality 10% put solid state fermentation in the installation for fermenting in the raw material after sterilization, it is 20 ~ 50cm that thickness is put in the shop, 25 ~ 50 ℃ of fermentation temperatures, fermentation time is 48 ~ 96 hours, described liquid spawn is made up of saccharomycete, bacillus and Alcaligenes, the volume ratio of saccharomycete, bacillus and Alcaligenes is 70:20:10, and the unit of activity of each bacterial classification is 10
10~ 10
14Cfu/ml; Described solid spawn is made up of black-koji mould, aspergillus oryzae and head mold, and the mass ratio of black-koji mould, aspergillus oryzae and head mold is 60:30:30, and the unit of activity of each bacterial classification is 10
10~ 10
14Cfu/g;
C. after fermentation is finished material is carried out drying and pulverize getting final product, the dry materials temperature is not higher than 50 ℃.
3. according to the production method of the described laying hen of claim 2, it is characterized in that described liquid spawn is to cultivate as follows to form with biological fermentation feed:
1.. scrape lawn or the spore of getting cultivation with oese, inserting 500ml under aseptic condition is equipped with in the triangular flask of the sterilized first order seed culture medium of 250ml, shaking table shaken cultivation 24 ~ 28h under 37 ℃ of conditions, promptly get primary seed solution, described first order seed culture medium is by beef extract 5g, peptone 10g, NaCl 5g, K2HPO
4The liquid of pH7.0 ~ 7.3 that 1g and water 1000ml constitute;
2.. the primary seed solution that obtains is linked into 2500ml by 10% inoculum concentration is equipped with in the triangular flask of the sterilized secondary seed medium of 1000ml, 37 ℃ of shaking table shaken cultivation 48h, described secondary seed medium is made of beef extract, peptone, brown sugar or molasses, NaCl and water, each raw material mass ratio is a beef extract 0.01, peptone 0.2, brown sugar or molasses 15, NaCl0.2, water 100.
4. according to the production method of the described laying hen of claim 3, it is characterized in that described solid spawn is to cultivate as follows to form with biological fermentation feed:
1.. scrape lawn or the spore of getting cultivation with oese, inserting 500ml under aseptic condition is equipped with in the triangular flask of the sterilized first order seed culture medium of 250ml, shaking table shaken cultivation 24 ~ 28h under 37 ℃ of conditions, promptly get primary seed solution, described first order seed culture medium is made of potato 200g, sucrose 20g and water 1000ml, be potato to be smashed add that poach is ripe, mix with sucrose and water after the filtered through gauze, autoclaving again;
2.. with dregs of beans, KH
2PO
4, MgSO
4And water mixes and sterilization, promptly makes the secondary seed solid medium, dregs of beans, KH
2PO
4, MgSO
4And the mass ratio of water is 100:0.2:0.05:50 ~ 60;
3.. the secondary seed solid medium of sterilization is transferred to sort of quyi chamber, and according to stirring after the 10% inoculum concentration access primary seed solution, keeping water content is 60%, and the generation spore that continuously ferments in bamboo basket gets final product.
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Cited By (4)
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| CN104686788A (en) * | 2013-12-04 | 2015-06-10 | 沈阳金海隆兴农牧科技有限公司 | Fermented feed and preparation process thereof |
| CN105146202A (en) * | 2015-09-23 | 2015-12-16 | 辽宁爱普罗斯饲料有限公司 | Production method for producing livestock and poultry feed by fermenting pumpkin bailers or tender pumpkins with probiotics and application |
| CN105285472A (en) * | 2015-11-20 | 2016-02-03 | 刘惠强 | Nutritious layer feed and preparation method thereof |
| CN105494948A (en) * | 2016-01-04 | 2016-04-20 | 梁丽莉 | Full-value passion fruit biological feed and preparing method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104686788A (en) * | 2013-12-04 | 2015-06-10 | 沈阳金海隆兴农牧科技有限公司 | Fermented feed and preparation process thereof |
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| CN105494948A (en) * | 2016-01-04 | 2016-04-20 | 梁丽莉 | Full-value passion fruit biological feed and preparing method thereof |
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| CN102106487B (en) | 2012-12-05 |
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