CN104686788A - Fermented feed and preparation process thereof - Google Patents
Fermented feed and preparation process thereof Download PDFInfo
- Publication number
- CN104686788A CN104686788A CN201310657357.2A CN201310657357A CN104686788A CN 104686788 A CN104686788 A CN 104686788A CN 201310657357 A CN201310657357 A CN 201310657357A CN 104686788 A CN104686788 A CN 104686788A
- Authority
- CN
- China
- Prior art keywords
- fermented feed
- wheat bran
- bacterium
- pulp
- kinds
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
Belonging to the field of feed and its preparation technologies, the invention in particular relates to a fermented feed and a preparation process thereof. The fermented feed includes 1000g-1240g of soybean meal, 200-440g of wheat bran, and primary corn pulp, wherein the primary corn pulp contains 96-122g of corn pulp protein and 192-244g of a dry matter. The preparation process of the fermented feed includes the steps of strain activation, solid inoculant preparation, and koji plate fermentation. The invention provides the cheap and crude protein rich fermented feed and the simple and effective fermented feed preparation process.
Description
Technical field
The invention belongs to feed and preparation technology field thereof, particularly relate to a kind of fermented feed and preparation technology thereof.
Background technology
Protein is a kind of extremely important macromolecular compound in organism, it be closely connected with life and with vital movement various forms of in organism together with material, it is the material base of life.All important component parts in each Cell and organism body in organism have protein to participate in.The protein eaten is hydrolyzed into amino acid in vivo after digestion, and amino acid by the protein after absorbing again needed for synthesising biological body, thus maintains growing and various metabolic activity of organism.High protein feed is conducive to the egg production and the meat yield that improve poultry and livestock, so the demand of livestock and poultry breeding industry to high protein feed is larger.But the price comparison of high protein feed sold on the market is at present high.In view of the demand, the feed how producing a kind of protein content high and cheap becomes technical barrier.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of fermented feed, it is characterized in that: comprise dregs of beans 1000g-1240g, wheat bran 200-440g, corn primary-pulp, the corn steep liquor albumen 96-122g wherein comprised in corn primary-pulp and dry 192-244g.
Describedly preferably consist of the corn steep liquor protein 11 0g and dry 220g that comprise in dregs of beans 1120g, wheat bran 320g, corn primary-pulp.
In order to solve the problems of the technologies described above, the invention provides a kind of preparation technology of fermented feed, it is characterized in that:
The activation of step one, bacterial classification: select saccharomycete, head mold, aspergillus oryzae three kinds of bacterium as bacterial classification, and three kinds of bacterium are inoculated on respective culture medium respectively carry out activation process, obtain the activated spawn of three kinds of bacterium;
The preparation of step 2, solid fungicide: the solid medium making three kinds of bacterium, is inoculated into the activated spawn of obtain in step one three kinds of bacterium respectively on three kinds of bacterium solid medium separately and cultivates, obtain the solid fungicide of three kinds of bacterium;
Step 3, koji plate fermentation: make the koji tray culture medium containing dregs of beans, wheat bran, corn primary-pulp, the solid fungicide of obtain in step 2 three kinds of bacterium is inoculated in koji tray culture medium, then carries out koji plate fermentation, obtain fermented feed.
In described step one, saccharomycete and head mold all select malt extract medium to carry out activation process, and aspergillus oryzae selects Czapek's medium to carry out activation process.
In described step 2, the manufacturing process of saccharomycete solid medium is: take wheat bran 12-16g, flour 1-3g, rice bran 1-3g, dregs of beans 1-3g, and mixing, puts into autoclave and carry out sterilization treatment; The manufacturing process of head mold solid medium is: take wheat bran 12-16g, flour 2-6g, rice bran 1-3g, and mixing, puts into autoclave and carry out sterilization treatment; The manufacturing process of aspergillus oryzae solid medium is: take wheat bran 14-18g, rice bran 1-3g, ground rice 1-3g, and mixing, puts into autoclave and carry out sterilization treatment.
In described step 2, the manufacturing process of preferred yeast bacterium solid medium is: take wheat bran 14g, flour 2g, rice bran 2g, dregs of beans 2g, and mixing, puts into autoclave and carry out sterilization treatment; The manufacturing process of preferred head mold solid medium is: take wheat bran 14g, flour 4g, rice bran 2g, and mixing, puts into autoclave and carry out sterilization treatment; The manufacturing process of preferred aspergillus oryzae solid medium is: take wheat bran 16g, rice bran 2g, ground rice 2g, and mixing, puts into autoclave and carry out sterilization treatment.
In described step 3, the manufacturing process of koji tray culture medium is: accurately take dregs of beans 1000-1240g, wheat bran 200-440g, accurately measure corn primary-pulp (adjusting PH to neutral) and the corn steep liquor albumen comprised in corn primary-pulp is controlled to control at 192-244g at 96-122g and dry, PH to 5.0-6.0 is adjusted after being mixed, infiltrate 20-40min, 121 DEG C of high-temperature sterilization 30min, take out when wild Oryza species temperature is down to about 40 DEG C and add ammonium sulfate 10-30g, urea 10-30g, glacial acetic acid 4-8ml, mixing.
In described step 3, the manufacturing process of preferred koji tray culture medium is: accurately take dregs of beans 1120g, wheat bran 320g, corn primary-pulp (adjust PH to neutral) controlled to control at 220g at 110g and dry by the corn steep liquor albumen comprised in corn primary-pulp, PH to 5.5 is adjusted after being mixed, infiltrate 30min, 121 DEG C of high-temperature sterilization 30min, take out when wild Oryza species temperature is down to about 40 DEG C and add ammonium sulfate 20g, urea 20g, glacial acetic acid 6ml, mixing.
In described step 3, the temperature of koji plate fermentation is 25 DEG C-36 DEG C, and humidity is 80%-90%, and fermentation time is 50-70h.
In described step 3, the temperature of preferred koji plate fermentation is 30.5 DEG C, and humidity is 85%, and fermentation time is 61h.
Beneficial effect of the present invention is: the invention provides a kind of fermented feed and preparation technology thereof, dregs of beans is comprised in fermented feed, wheat bran, corn primary-pulp, in dregs of beans, the content of crude protein reaches 30%-50%, and it also has and is easy to absorb, diseases prevention, improve efficiency of feed utilization and somatotrophic effect, containing abundant dietary fiber and containing B family vitamin in wheat bran, selenium, the mineral matters such as magnesium, containing abundant soluble protein in corn primary-pulp, auxin and some precursor substances, nutritious, be conducive to the egg production and the meat yield that improve poultry and livestock, various cheaper starting materials is easy to get, production cost is low, feed by fermentation, the crude protein content after fermentation be fermentation before 1.5 times of crude protein content, greatly improve the content of crude protein, the proportioning of fermented feed and preparation technology simply, are easy to promote, are of great practical significance.In a word, the invention provides a kind of cheaper starting materials, the preparation technology of fermented feed that crude protein content enriches and a kind of simple and effective fermented feed.
Detailed description of the invention
The invention will be further described below:
Embodiment 1
A kind of fermented feed, comprises dregs of beans, wheat bran, corn primary-pulp, wherein, and dregs of beans 1100g, wheat bran 340g, corn primary-pulp, the corn steep liquor albumen 96g wherein comprised in corn primary-pulp and dry 192g;
A kind of preparation technology of fermented feed comprises the steps:
First, article used for experiment and glass apparatus are put into 140 DEG C of drying box hot air sterilizations;
The activation of step one, bacterial classification
1, the making of culture medium:
(1) malt extract medium: accurately measure brewer's wort 1000ml, adds 2% agar 20g, adjusts PH to neutral, get it to add in right amount in test tube, test tube is put into autoclave 121 DEG C of sterilizings 30 minutes, take out bevel, after it solidifies, put into 30 DEG C of incubators, cultivate 72 hours;
(2) Czapek's medium: accurately take sodium nitrate 1.5g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.25g, potassium chloride 0.25g, ferric sulfate 0.005g, sucrose 10g, agar 10g, distilled water 500ml, adjust PH to neutral, be heated to milky liquid, get it to add in right amount in test tube, test tube is put into autoclave 121 DEG C of sterilizings 30 minutes, take out bevel, after it solidifies, put into 30 DEG C of incubators, cultivate 72 hours;
2, inoculate:
Select saccharomycete, head mold, aspergillus oryzae three kinds of bacterium as bacterial classification, three kinds of bacterium are inoculated in the superclean bench of desinfection chamber, wherein, saccharomycete, head mold are inoculated on malt extract medium, aspergillus oryzae is inoculated on Czapek's medium, put into 30 DEG C of incubators after having inoculated to cultivate 72 hours, obtain the activated spawn of three kinds of bacterium;
The preparation of step 2, solid fungicide
1, the making of culture medium:
The manufacturing process of saccharomycete solid medium is: take wheat bran 13g, flour 3g, rice bran 2g, dregs of beans 2g, and mixing, puts into autoclave 121 DEG C of sterilizings 30 minutes;
The manufacturing process of head mold solid medium is: take wheat bran 14g, flour 3g, rice bran 3g, and mixing, puts into autoclave 121 DEG C of sterilizings 30 minutes;
The manufacturing process of aspergillus oryzae solid medium is: take wheat bran 16g, rice bran 1g, ground rice 3g, and mixing, puts into autoclave 121 DEG C of sterilizings 30 minutes;
2, inoculate:
The activated spawn of the saccharomycete obtained in optional step one, head mold, aspergillus oryzae, three kinds of activated spawn are inoculated in the superclean bench of desinfection chamber on the corresponding solid medium of each bacterium, put into 25-35 DEG C of incubator after having inoculated and cultivate 60-84 hour, obtain the solid fungicide of three kinds of bacterial classifications;
Step 3, koji plate fermentation
1, the making of koji tray culture medium:
Accurately take dregs of beans 1100g, wheat bran 340g, corn primary-pulp (adjusting PH to neutral) 500ml, wherein, the content of the corn steep liquor albumen in corn primary-pulp is 17-18%, Baume is 16-18, and the content of dry is 34-36%, adjusts PH to 5.5 after being mixed, infiltrate 30min, 121 DEG C of high-temperature sterilization 30min, take out when wild Oryza species temperature is down to about 40 DEG C and add ammonium sulfate 25g, urea 15g, glacial acetic acid 6ml, mixing;
2, inoculate:
The solid fungicide of the saccharomycete obtained in optional step two, head mold, aspergillus oryzae, is inoculated on same koji tray culture medium by three kinds of solid fungicides each picking 0.1-0.3g in the superclean bench of desinfection chamber, forms experimental group culture medium;
Not inoculate the koji tray culture medium of microbial inoculum as blank group culture medium;
3, hatch:
Experimental group culture medium and blank group culture medium are put into incubator, and incubator temperature is adjusted to 32.5 DEG C, humidity 90%, hatches 50 hours;
Step 4, detection
After experimental group culture medium and blank group culture medium are hatched 50 hours, take out, detected the content of crude protein respectively by triumphant formula nitriding, experimental group culture medium is increased to 1.47 times compared with the crude protein content of blank group culture medium;
Embodiment 2
A kind of fermented feed, comprises dregs of beans, wheat bran, corn primary-pulp, wherein, and dregs of beans 1100g, wheat bran 340g, corn primary-pulp, the corn steep liquor protein 10 4g wherein comprised in corn primary-pulp and dry 208g;
A kind of preparation technology of fermented feed comprises the steps:
First, article used for experiment and glass apparatus are put into 140 DEG C of drying box hot air sterilizations;
The activation of step one, bacterial classification
1, the making of culture medium:
(1) malt extract medium: accurately measure brewer's wort 1000ml, adds 2% agar 20g, adjusts PH to neutral, get it and add test tube in right amount, test tube is put into autoclave 121 DEG C of sterilizings 30 minutes, take out bevel, after it solidifies, put into 30 DEG C of incubators, cultivate 72 hours;
(2) Czapek's medium: accurately take sodium nitrate 1.5g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.25g, potassium chloride 0.25g, ferric sulfate 0.005g, sucrose 10g, agar 10g, distilled water 500ml, adjust PH to neutral, be heated to milky liquid, get it and add test tube in right amount, test tube is put into autoclave 121 DEG C of sterilizings 30 minutes, take out bevel, after it solidifies, put into 30 DEG C of incubators, cultivate 72 hours;
2, inoculate:
Select saccharomycete, head mold, aspergillus oryzae three kinds of bacterium as bacterial classification, three kinds of bacterial classifications are inoculated in the superclean bench of desinfection chamber, wherein, saccharomycete, head mold are inoculated on malt extract medium, aspergillus oryzae is inoculated on Czapek's medium, put into 30 DEG C of incubators after having inoculated to cultivate 72 hours, obtain the activated spawn of three kinds of bacterium;
The preparation of step 2, solid fungicide
1, the making of culture medium:
The manufacturing process of saccharomycete solid medium is: take wheat bran 12g, flour 3g, rice bran 3g, dregs of beans 2g, and mixing, puts into autoclave 121 DEG C of sterilizings 30 minutes;
The manufacturing process of head mold solid medium is: take wheat bran 15g, flour 4g, rice bran 1g, and mixing, puts into autoclave 121 DEG C of sterilizings 30 minutes;
The manufacturing process of aspergillus oryzae solid medium is: take wheat bran 17g, rice bran 2g, ground rice 1g, and mixing, puts into autoclave 121 DEG C of sterilizings 30 minutes;
2, inoculate:
The activated spawn of the saccharomycete obtained in optional step one, head mold, aspergillus oryzae, three kinds of activated spawn are inoculated in the superclean bench of desinfection chamber on the corresponding solid medium of each bacterium, put into 25-35 DEG C of incubator after having inoculated and cultivate 60-84 hour, obtain the solid fungicide of three kinds of bacterial classifications;
Step 3, koji plate fermentation
1, the making of koji tray culture medium:
Accurately take dregs of beans 1200g, wheat bran 240g, corn primary-pulp (adjusting PH to neutral) 530ml, wherein, the content of the corn steep liquor albumen in corn primary-pulp is 17-18%, Baume is 16-18, the content of dry is 34-36%, adjusts PH to 5.7 after being mixed, and infiltrates 35min, 121 DEG C of high-temperature sterilization 30min, add ammonium sulfate 14g, urea 26g when taking-up wild Oryza species temperature is down to about 40 DEG C, glacial acetic acid 7ml mix;
2, inoculate:
The solid fungicide of the saccharomycete obtained in optional step two, head mold, aspergillus oryzae, is inoculated on same koji tray culture medium by three kinds of solid fungicides each picking 0.1-0.3g in the superclean bench of desinfection chamber, forms experimental group culture medium;
Not inoculate the koji tray culture medium of microbial inoculum as blank group culture medium;
3, hatch:
Experimental group culture medium and blank group culture medium are put into incubator, and incubator temperature is adjusted to 32.5 DEG C, humidity 90%, hatches 50 hours;
Step 4, detection
After experimental group culture medium and blank group culture medium are hatched 60 hours, take out, detected the content of crude protein respectively by triumphant formula nitriding, the protein content of feed after utilizing inspection fermentation, experimental group culture medium is increased to 1.49 times compared with the crude protein content of blank group culture medium.
Embodiment 3
A kind of fermented feed, comprises dregs of beans, wheat bran, corn primary-pulp, wherein, and dregs of beans 1200g, wheat bran 240g, corn primary-pulp, the corn steep liquor protein 11 4g wherein comprised in corn primary-pulp and dry 228g;
A kind of preparation technology of fermented feed comprises the steps:
First, article used for experiment and glass apparatus are put into 140 DEG C of drying box hot air sterilizations;
The activation of step one, bacterial classification
1, the making of culture medium:
(1) malt extract medium: accurately measure brewer's wort 1000ml, adds 2% agar 20g, adjusts PH to neutral, get it and add test tube in right amount, test tube is put into autoclave 121 DEG C of sterilizings 30 minutes, take out bevel, after it solidifies, put into 30 DEG C of incubators, cultivate 72 hours;
(2) Czapek's medium: accurately take sodium nitrate 1.5g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.25g, potassium chloride 0.25g, ferric sulfate 0.005g, sucrose 10g, agar 10g, distilled water 500ml, adjust PH to neutral, be heated to milky liquid, get it and add test tube in right amount, test tube is put into autoclave 121 DEG C of sterilizings 30 minutes, take out bevel, after it solidifies, put into 30 DEG C of incubators, cultivate 72 hours;
2, inoculate:
Select saccharomycete, head mold, aspergillus oryzae three kinds of bacterium as bacterial classification, three kinds of bacterium are inoculated in the superclean bench of desinfection chamber, wherein, saccharomycete, head mold are inoculated on malt extract medium, aspergillus oryzae is inoculated on Czapek's medium, put into 30 DEG C of incubators after having inoculated to cultivate 72 hours, obtain the activated spawn of three kinds of bacterium;
The preparation of step 2, solid fungicide
1, the making of culture medium:
The manufacturing process of saccharomycete solid medium is: take wheat bran 12g, flour 3g, rice bran 3g, dregs of beans 2g, and mixing, puts into autoclave sterilizing 30 minutes;
The manufacturing process of head mold solid medium is: take wheat bran 14g, flour 4g, rice bran 2g, and mixing, puts into autoclave sterilizing 30 minutes;
The manufacturing process of aspergillus oryzae solid medium is: take wheat bran 15g, rice bran 2g, ground rice 3g, and mixing, puts into autoclave sterilizing 30 minutes;
2, inoculate:
The activated spawn of the saccharomycete obtained in optional step one, head mold, aspergillus oryzae, three kinds of activated spawn are inoculated in the superclean bench of desinfection chamber on the corresponding solid medium of each bacterium, put into 25-35 DEG C of incubator after having inoculated and cultivate 60-84 hour, obtain the solid fungicide of three kinds of bacterial classifications;
Step 3, koji plate fermentation
1, the making of koji tray culture medium:
Accurately take dregs of beans 1200g, wheat bran 240g, corn primary-pulp (adjusting PH to neutral) 580ml, wherein, the content of the corn steep liquor albumen in corn primary-pulp is 17-18%, Baume is 16-18, the content of dry is 34-36%, adjusts PH to 5.7 after being mixed, and infiltrates 30min, 121 DEG C of high-temperature sterilization 30min, add ammonium sulfate 20g, urea 20g when taking-up wild Oryza species temperature is down to about 40 DEG C, glacial acetic acid 6ml mix;
2, inoculate:
Three kinds of solid fungicides are all inoculated on same koji tray culture medium at the superclean bench of desinfection chamber each picking 0.1-0.3g by the solid fungicide of the saccharomycete obtained in optional step two, head mold, aspergillus oryzae, form experimental group culture medium;
Not inoculate the koji tray culture medium of microbial inoculum as blank group culture medium;
3, hatch:
Experimental group culture medium and blank group culture medium are put into incubator, and incubator temperature is adjusted to 30.5 DEG C, humidity 80%, hatches 63 hours;
Step 4, detection
After experimental group culture medium and blank group culture medium are hatched 63 hours, take out, detected the content of crude protein respectively by triumphant formula nitriding, experimental group culture medium is increased to 1.50 times compared with the crude protein of blank group culture medium.
Embodiment 4
A kind of fermented feed, comprises dregs of beans, wheat bran, corn primary-pulp, wherein, and dregs of beans 1100g, wheat bran 340g, corn primary-pulp, the corn steep liquor protein 12 2g wherein comprised in corn primary-pulp and dry 244g;
A kind of preparation technology of fermented feed comprises the steps:
First, article used for experiment and glass apparatus are put into 140 DEG C of drying box hot air sterilizations;
The activation of step one, bacterial classification
1, the making of culture medium:
(1) malt extract medium: accurately measure brewer's wort 1000ml, adds 2% agar 20g, adjusts PH to neutral, get it and add test tube in right amount, test tube is put into autoclave 121 DEG C of sterilizings 30 minutes, take out bevel, after it solidifies, put into 30 DEG C of incubators, cultivate 72 hours;
(2) Czapek's medium: accurately take sodium nitrate 1.5g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.25g, potassium chloride 0.25g, ferric sulfate 0.005g, sucrose 10g, agar 10g, distilled water 500ml, adjust PH to neutral, be heated to milky liquid, get it and add test tube in right amount, test tube is put into autoclave 121 DEG C of sterilizings 30 minutes, take out bevel, after it solidifies, put into 30 DEG C of incubators, cultivate 72 hours;
2, inoculate:
Select saccharomycete, head mold, aspergillus oryzae three kinds of bacterium as bacterial classification, three kinds of bacterium are inoculated in the superclean bench of desinfection chamber, wherein, saccharomycete, head mold are inoculated on malt extract medium, aspergillus oryzae is inoculated on Czapek's medium, put into 30 DEG C of incubators after having inoculated to cultivate 72 hours, obtain the activated spawn of three kinds of bacterium;
The preparation of step 2, solid fungicide
1, the making of culture medium:
The manufacturing process of saccharomycete solid medium is: take wheat bran 14g, flour 2g, rice bran 2g, dregs of beans 2g, and mixing, puts into autoclave 121 DEG C of sterilizings 30 minutes;
The manufacturing process of head mold solid medium is: take wheat bran 13g, flour 4g, rice bran 3g, and mixing, puts into autoclave 121 DEG C of sterilizings 30 minutes;
The manufacturing process of aspergillus oryzae solid medium is: take wheat bran 16g, rice bran 2g, ground rice 2g, and mixing, puts into autoclave 121 DEG C of sterilizings 30 minutes;
2, inoculate:
The activated spawn of the saccharomycete obtained in optional step one, head mold, aspergillus oryzae, three kinds of activated spawn are inoculated in the superclean bench of desinfection chamber on the corresponding solid medium of each bacterium, put into 25-35 DEG C of incubator after having inoculated and cultivate 60-84 hour, obtain the solid fungicide of three kinds of bacterium;
Step 3, koji plate fermentation
1, the making of koji tray culture medium:
Accurately take dregs of beans 1100g, wheat bran 340g, corn primary-pulp (adjusting PH to neutral) 600ml, wherein, the content of the corn steep liquor albumen in corn primary-pulp is 17-18%, Baume is 16-18, and the content of dry is 34-36%, adjusts PH to 5.5 after being mixed, infiltrate 30min, 121 DEG C of high-temperature sterilization 30min, take out when wild Oryza species temperature is down to about 40 DEG C and add ammonium sulfate 18g, urea 22g, glacial acetic acid 7ml, mixing;
2, inoculate:
The solid fungicide of the saccharomycete obtained in optional step two, head mold, aspergillus oryzae, is inoculated on same koji tray culture medium by three kinds of solid fungicides each picking 0.1-0.3g in the superclean bench of desinfection chamber, forms experimental group culture medium;
Not inoculate the koji tray culture medium of microbial inoculum as blank group culture medium;
3, hatch:
Experimental group culture medium and blank group culture medium are put into incubator, and incubator temperature is adjusted to 31.5 DEG C, humidity 88%, hatches 61 hours;
Step 4, detection
After experimental group culture medium and blank group culture medium are hatched 61 hours, take out, detected the content of crude protein respectively by triumphant formula nitriding, experimental group culture medium is increased to 1.50 times compared with the crude protein content of blank group culture medium.
Embodiment 5
A kind of fermented feed, comprises dregs of beans, wheat bran, corn primary-pulp, wherein, and dregs of beans 1120g, wheat bran 320g, corn primary-pulp, the corn steep liquor protein 11 1g wherein comprised in corn primary-pulp and dry 222g;
A kind of preparation technology of fermented feed comprises the steps:
First, article used for experiment and glass apparatus are put into 140 DEG C of drying box hot air sterilizations;
The activation of step one, bacterial classification
1, the making of culture medium:
(1) malt extract medium: accurately measure brewer's wort 1000ml, adds 2% agar 20g, adjusts PH to neutral, get it and add test tube in right amount, test tube is put into autoclave 121 DEG C of sterilizings 30 minutes, take out bevel, after it solidifies, put into 30 DEG C of incubators, cultivate 72 hours;
(2) Czapek's medium: accurately take sodium nitrate 1.5g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.25g, potassium chloride 0.25g, ferric sulfate 0.005g, sucrose 10g, agar 10g, distilled water 500ml, adjust PH to neutral, be heated to milky liquid, get it and add test tube in right amount, test tube is put into autoclave 121 DEG C of sterilizings 30 minutes, take out bevel, after it solidifies, put into 30 DEG C of incubators, cultivate 72 hours;
2, inoculate:
Select saccharomycete, head mold, aspergillus oryzae three kinds of bacterium as bacterial classification, three kinds of bacterium are inoculated in the superclean bench of desinfection chamber, wherein, saccharomycete, head mold are inoculated on malt extract medium, aspergillus oryzae is inoculated on Czapek's medium, put into 30 DEG C of incubators after having inoculated to cultivate 72 hours, obtain the activated spawn of three kinds of bacterium;
The preparation of step 2, solid fungicide
1, the making of culture medium:
The manufacturing process of saccharomycete solid medium is: take wheat bran 14g, flour 2g, rice bran 2g, dregs of beans 2g, and mixing, puts into autoclave 121 DEG C of sterilizings 30 minutes;
The manufacturing process of head mold solid medium is: take wheat bran 14g, flour 4g, rice bran 2g, and mixing, puts into autoclave 121 DEG C of sterilizings 30 minutes;
The manufacturing process of aspergillus oryzae solid medium is: take wheat bran 16g, rice bran 2g, ground rice 2g, and mixing, puts into autoclave 121 DEG C of sterilizings 30 minutes;
2, inoculate:
The activated spawn of the saccharomycete obtained in optional step one, head mold, aspergillus oryzae, three kinds of activated spawn are inoculated in the superclean bench of desinfection chamber on the corresponding solid medium of each bacterium, put into 30 DEG C of incubators after having inoculated to cultivate 72 hours, obtain the solid fungicide of three kinds of bacterium;
Step 3, koji plate fermentation
1, the making of koji tray culture medium:
Accurately take dregs of beans 1120g, wheat bran 320g, corn primary-pulp (adjusting PH to neutral) 560ml, wherein, the content of the corn steep liquor albumen in corn primary-pulp is 17-18%, Baume is 16-18, and the content of dry is 34-36%, adjusts PH to 5.5 after being mixed, infiltrate 30min, 121 DEG C of high-temperature sterilization 30min, take out when wild Oryza species temperature is down to about 40 DEG C and add ammonium sulfate 20g, urea 20g, glacial acetic acid 6ml, mixing;
2, inoculate:
The solid fungicide of the saccharomycete obtained in optional step two, head mold, aspergillus oryzae, is inoculated on same koji tray culture medium by three kinds of solid fungicides each picking 0.1-0.3g in the superclean bench of desinfection chamber, forms experimental group culture medium;
Not inoculate the koji tray culture medium of microbial inoculum as blank group culture medium;
3, hatch:
Experimental group culture medium and blank group culture medium are put into incubator, and incubator temperature is adjusted to 30.5 DEG C, humidity 85%, hatches 61 hours;
Step 4, detection
After experimental group culture medium and blank group culture medium are hatched 61 hours, take out, detected the content of crude protein respectively by triumphant formula nitriding, experimental group culture medium is increased to 1.52 times compared with the crude protein content of blank group culture medium.
Above 5 embodiments of the present invention have been described in detail, but described content being only preferred embodiment of the present invention, can not being considered to for limiting practical range of the present invention.All equalizations done according to the present patent application scope change and improve, and all should still belong within patent covering scope of the present invention.
Claims (10)
1. a fermented feed, is characterized in that: comprise dregs of beans 1000g-1240g, wheat bran 200-440g, corn primary-pulp, the corn steep liquor albumen 96-122g wherein comprised in corn primary-pulp and dry 192-244g.
2. a kind of fermented feed according to claim 1, is characterized in that: describedly preferably consist of the corn steep liquor protein 11 0g and dry 220g that comprise in dregs of beans 1120g, wheat bran 320g, corn primary-pulp.
3. a preparation technology for fermented feed, is characterized in that:
The activation of step one, bacterial classification: select saccharomycete, head mold, aspergillus oryzae three kinds of bacterium as bacterial classification, and three kinds of bacterium are inoculated into respectively separately corresponding culture medium carry out activation process, obtain the activated spawn of three kinds of bacterium;
The preparation of step 2, solid fungicide: make the solid medium of three kinds of bacterium, is inoculated into three kinds of bacterium respectively and accordingly solid medium is cultivated separately, obtain the solid fungicide of three kinds of bacterium by the activated spawn of obtain in step one three kinds of bacterium;
Step 3, koji plate fermentation: make the koji tray culture medium containing dregs of beans, wheat bran, corn primary-pulp, the solid fungicide of obtain in step 2 three kinds of bacterium is inoculated in koji tray culture medium, then carries out koji plate fermentation, obtain fermented feed.
4. the preparation technology of a kind of fermented feed according to claim 3, is characterized in that: in described step one, saccharomycete and head mold all select malt extract medium to carry out activation process, and aspergillus oryzae selects Czapek's medium to carry out activation process.
5. the preparation technology of a kind of fermented feed according to claim 3, it is characterized in that: in described step 2, the manufacturing process of saccharomycete solid medium is: take wheat bran 12-16g, flour 1-3g, rice bran 1-3g, dregs of beans 1-3g, mixing, puts into autoclave and carries out sterilization treatment; The manufacturing process of head mold solid medium is: take wheat bran 12-16g, flour 2-6g, rice bran 1-3g, and mixing, puts into autoclave and carry out sterilization treatment; The manufacturing process of aspergillus oryzae solid medium is: take wheat bran 14-18g, rice bran 1-3g, ground rice 1-3g, and mixing, puts into autoclave and carry out sterilization treatment.
6. the preparation technology of a kind of fermented feed according to claim 5, it is characterized in that: in described step 2, the manufacturing process of preferred yeast bacterium solid medium is: take wheat bran 14g, flour 2g, rice bran 2g, dregs of beans 2g, mixing, puts into autoclave and carries out sterilization treatment; The manufacturing process of preferred head mold solid medium is: take wheat bran 14g, flour 4g, rice bran 2g, and mixing, puts into autoclave and carry out sterilization treatment; The solid medium manufacturing process of preferred aspergillus oryzae is: take wheat bran 16g, rice bran 2g, ground rice 2g, and mixing, puts into autoclave and carry out sterilization treatment.
7. the preparation technology of a kind of fermented feed according to claim 3, it is characterized in that: in described step 3, the manufacturing process of koji tray culture medium is: take dregs of beans 1000-1240g, wheat bran 200-440g, accurately measure corn primary-pulp (adjusting PH to neutral) and the corn steep liquor albumen comprised in corn primary-pulp is controlled to control at 192-244g at 96-122g and dry, PH to 5.0-6.0 is adjusted after being mixed, infiltrate 20-40min, 121 DEG C of high-temperature sterilization 30min, take out when wild Oryza species temperature is down to about 40 DEG C and add ammonium sulfate 10-30g, urea 10-30g, glacial acetic acid 4-8ml, mixing.
8. the preparation technology of a kind of fermented feed according to claim 7, it is characterized in that: in described step 3, the manufacturing process of preferred koji tray culture medium is: accurately take dregs of beans 1120g, wheat bran 320g, corn primary-pulp (adjust PH to neutral) controlled to control at 220g at 110g and dry by the corn steep liquor albumen comprised in corn primary-pulp, PH to 5.5 is adjusted after being mixed, infiltrate 30min, 121 DEG C of high-temperature sterilization 30min, take out when wild Oryza species temperature is down to about 40 DEG C and add ammonium sulfate 20g, urea 20g, glacial acetic acid 6ml, mixing.
9. the preparation technology of a kind of fermented feed according to claim 3, is characterized in that: in described step 3, the temperature of koji plate fermentation is 25 DEG C-36 DEG C, and humidity is 80%-90%, and fermentation time is 50-70h.
10. the preparation technology of a kind of fermented feed according to claim 9, is characterized in that: in described step 3, the temperature of preferred koji plate fermentation is 30.5 DEG C, and humidity is 85%, and fermentation time is 61h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310657357.2A CN104686788A (en) | 2013-12-04 | 2013-12-04 | Fermented feed and preparation process thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310657357.2A CN104686788A (en) | 2013-12-04 | 2013-12-04 | Fermented feed and preparation process thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104686788A true CN104686788A (en) | 2015-06-10 |
Family
ID=53334683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310657357.2A Pending CN104686788A (en) | 2013-12-04 | 2013-12-04 | Fermented feed and preparation process thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104686788A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105124136A (en) * | 2015-09-24 | 2015-12-09 | 山东祥维斯生物科技股份有限公司 | Fermented soybean meal production process suitable for large-scale production |
| CN108850692A (en) * | 2018-06-20 | 2018-11-23 | 西南大学 | A kind of composite bait for fish culture |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101513220A (en) * | 2009-03-24 | 2009-08-26 | 中国农业科学院油料作物研究所 | Preparation method of protein feed in oilseed dregs |
| CN101715875A (en) * | 2009-12-15 | 2010-06-02 | 四川省畜牧科学研究院 | Protein feed prepared by adopting rapeseed dregs and preparation method thereof |
| CN101773203A (en) * | 2010-03-05 | 2010-07-14 | 中国农业科学院油料作物研究所 | Method for biologically improving oilseed residue |
| CN102106487A (en) * | 2011-02-01 | 2011-06-29 | 大连韩伟养鸡有限公司 | Biological fermentation feed for laying chickens and production method |
| CN102204632A (en) * | 2011-05-12 | 2011-10-05 | 成都和谐生物科技有限公司 | Microbial feed additive for increasing livestock and poultry product quality and improving meat egg milk flavor and preparation method thereof |
| CN103330050A (en) * | 2013-05-10 | 2013-10-02 | 张永根 | Corn steep liquor and rice straw mixed fermentation feed production technology |
-
2013
- 2013-12-04 CN CN201310657357.2A patent/CN104686788A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101513220A (en) * | 2009-03-24 | 2009-08-26 | 中国农业科学院油料作物研究所 | Preparation method of protein feed in oilseed dregs |
| CN101715875A (en) * | 2009-12-15 | 2010-06-02 | 四川省畜牧科学研究院 | Protein feed prepared by adopting rapeseed dregs and preparation method thereof |
| CN101773203A (en) * | 2010-03-05 | 2010-07-14 | 中国农业科学院油料作物研究所 | Method for biologically improving oilseed residue |
| CN102106487A (en) * | 2011-02-01 | 2011-06-29 | 大连韩伟养鸡有限公司 | Biological fermentation feed for laying chickens and production method |
| CN102204632A (en) * | 2011-05-12 | 2011-10-05 | 成都和谐生物科技有限公司 | Microbial feed additive for increasing livestock and poultry product quality and improving meat egg milk flavor and preparation method thereof |
| CN103330050A (en) * | 2013-05-10 | 2013-10-02 | 张永根 | Corn steep liquor and rice straw mixed fermentation feed production technology |
Non-Patent Citations (2)
| Title |
|---|
| 甘在红等: "玉米深加工淀粉副产物的蛋白选择和应用", 《饲料与畜牧》 * |
| 田亚东等: "工业副产品饲料资源的开发和利用", 《河南畜牧兽医》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105124136A (en) * | 2015-09-24 | 2015-12-09 | 山东祥维斯生物科技股份有限公司 | Fermented soybean meal production process suitable for large-scale production |
| CN108850692A (en) * | 2018-06-20 | 2018-11-23 | 西南大学 | A kind of composite bait for fish culture |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103478683B (en) | A kind of fermentation type beef sauce and preparation method thereof | |
| CN102823726B (en) | It is a kind of improve cottonseed meal content and can detoxification biofermentation method | |
| CN101933441B (en) | Method for improving yield of straw mushroom | |
| CN104222668A (en) | Compound fish feed capable of improving body immunity | |
| CN102060578B (en) | Method for producing microecological foliar fertilizer by utilizing probiotics and microalgae | |
| CN102132881B (en) | Method for preparing lucid ganoderma rice food | |
| CN102429163A (en) | Method for preparing tartary buckwheat monascus and application thereof | |
| CN104247878A (en) | Compound pig feed capable of improving body immunity | |
| CN104017739B (en) | Complex micro organism fungicide, its preparation method and its application in high protein potato dreg fodder is produced | |
| CN104904498A (en) | Cultivation method of black fungi | |
| CN107624503B (en) | Shrimp feed additive and application thereof | |
| CN103387463A (en) | Cultivation method and cultivation culture material of black fungus | |
| CN103385350B (en) | Soft particle milk replacer containing high fructose corn syrup and preparation thereof | |
| CN102246890A (en) | Making method for producing biologic protein feeds by using corncobs | |
| CN103564199A (en) | Method for producing cow feed by multi-strain mixing solid-state fermentation | |
| CN105638241A (en) | Selenium-rich high-yield oyster mushroom cultivation method | |
| CN105053602A (en) | Mulberry pomace chicken feed and preparation method thereof | |
| CN109497266A (en) | A kind of method that multi-cultur es composite fermentation produces high-quality biological feedstuff | |
| CN103355477B (en) | Production method for feed through fermentation of soy sauce residues | |
| CN101756012A (en) | Method for producing high-lysine fermented feed through fermentation of vinegar residue using two-step method | |
| CN108432979A (en) | A kind of Australia freshwater lobster ecological feed | |
| CN102960538B (en) | Unicellular protein feed prepared from fermented dragon fruit peel and production method of unicellular protein feed | |
| CN110353087A (en) | The preparation method and fermented feed of rape stalk fermented feed | |
| CN104381615B (en) | Produce the method and product of aweto feed addictive | |
| CN103859149A (en) | Production technology of lactobacillus fermented soybean meal |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150610 |
|
| WD01 | Invention patent application deemed withdrawn after publication |