CN105124136A - Fermented soybean meal production process suitable for large-scale production - Google Patents
Fermented soybean meal production process suitable for large-scale production Download PDFInfo
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Abstract
The invention discloses a fermented soybean meal production process suitable for large-scale production and belongs to the technical field of soybean meal processing. The process comprises the steps of mixing tryptone, glucose, yeast extract and sodium chloride, sterilizing at high temperature, adding less-root rhizopus oryzae into a seed culture medium for culturing and preparing a seed culture medium; mixing bean meal, bran, sucrose and glycine betaine, adding water and uniformly mixing; sterilizing at high temperature and naturally cooling to prepare a fermented culture medium; inoculating the seed culture medium into the fermented culture medium and fermenting for culture; adding a glycine betaine solution in a spraying manner after fermenting, and stopping fermenting after spraying; drying and grinding; drying a fermented product, grinding and sieving to obtain a fermented bean meal finished product. According to the process, seed enlarged cultivation is performed, and stability of a production technology is realized; the glycine betaine is added in fermentation to increase the mixing uniformity of the materials; single less-root rhizopus oryzae strains are adopted to ferment the bean meal, so that the fermentation contamination probability is greatly reduced; the process is mature and stable and suitable for large-scale production.
Description
Technical field
The present invention relates to dregs of beans processing technique field, be specifically related to a kind of fermented bean dregs production technology strengthening ANFs degradation effect, increase palatability, stable applicable large-scale production.
Background technology
Dregs of beans is a kind of byproduct obtained after soybean extracting bean oil, and dregs of beans is generally in irregular fragment shape, and color is light yellow to light brown, and taste has roasting soybean fragrance; The main component of dregs of beans comprises: protein 40% ~ 48%, lysine 2.5% ~ 3.0%, tryptophan 0.6% ~ 0.7% and methionine 0.5% ~ 0.7%.Dregs of beans is that in 12 kinds of animals and plants oil meal feed products such as Cottonseed Meal, peanut meal, rapeseed dregs, output is maximum, the one that purposes is the widest; As a kind of high protein, dregs of beans is the primary raw material making livestock and poultry feed, is the good dietary protein source of fish and nonruminant.The several amino acids that dregs of beans includes is suitable for poultry or the pig demand for nutrition, experiment shows, when not needing additionally to add other animal protein, only contained in dregs of beans amino acid is just enough to the nutrition balancing poultry or pig, thus promotes the nutrient absorption of livestock; In fact, dregs of beans has become the benchmark product that other protein sources compares.
But, multiple ANFs is also there is in dregs of beans, as trypsin ihhibitor, phytic acid, soybean lectin, urase, compound sugar, lipoxidase, Soybean antigen protein (sensitizing factor) and goitrin etc., these ANFs can produce harmful effect to the absorption of dregs of beans Middle nutrition composition, metabolism and feeding animals health.In view of above-mentioned, need to process dregs of beans, to overcome the adverse effect that ANFs produces; Test finds, can effectively overcome this kind of defect by the fermented bean dregs after the process of modern biotechnology fermentation technique.
Fermented bean dregs has another name called biological peptide, biological soybean meal, bioactive micro peptide and Soybean Peptide, fermented bean dregs small molecular protein and little peptide content high, little peptide (below MW1000Dalton) content accounts for more than 70% of total protein; Become the best substitute of fish meal at present.This product utilizes modern biological project fermented bacterium technology to combine with the solid-fermented technique of Chinese tradition, with high-quality dregs of beans for primary raw material, microbe inoculation, eliminated the ANFs in dregs of beans to greatest extent by the fermentation of microorganism, degrading soybean protein is the little peptide protein sources of high-quality effectively; Fermented bean dregs is while purification antigen and ANFs, also produce the various active materials such as protease, non-starch polysaccharide enzyme, lactic acid, probio, oligopeptides, glutamic acid, lactic acid, vitamin, UGF (UGF) during the fermentation, make dregs of beans be converted into the higher functional feed of nutritive value.
The advantage that fermented bean dregs has is as follows:
1, fermented bean dregs has unique fermentation fragranced, and improve palatability, attractant is splendid, improves feed local flavor, and increase animal appetite, Long-Time Service forms hobby, improves animal feed intake, growth promoting effects, reduction material consumption;
2, research shows, protein is absorbed with little peptide in gastral catabolite major part, thus, in daily ration, add fermented bean dregs can improve proteopepsis absorption rate and reach more than 95%, greatly promote that nitrogen absorbs with the form of peptide, improve feed nitrogen to deposit in vivo, little peptide in enteron aisle easily and mineral element be combined into the chelate of solubility and promote the absorption of Ca, P, Cu, Zn, Fe, Mn, I, Se, Co etc., promotion growth of animal;
3, provide natural acidulant-lactic acid, acetic acid, fodder acidulant need not be added separately, reduce feed cost, improve digesting and assimilating of nutriment;
4, without antigen and ANFs, the trophism diarrhea of animal is solved;
5, the overall activity of conditioning and active cell and body, promotes the growth of animal intestinal fine hair, promotes young animal gastrointestinal function, improves disease resistance ability, promotes the healthy growth and development of animal;
6, the high-content biological active component contained in fermented bean dregs and the sugar being beneficial to utilization are rich in oligopeptides, vitamin and other somatomedins in enteron aisle or by after epithelium mucous membrane absorbed intact, the biological action of himself can be played in vivo, transport speed soon, not easily saturated, do not exist and compete with Amino Acid Absorption, the transhipment of free amino acid can also be promoted, absorb power consumption low, save energy, improve the utilization rate of albumen; Containing a large amount of digestion class being easy to dissolve sugar in fermented bean dregs, can rapidly for animal body absorb, absorption rate is fast, and contained disaccharide can enter TCA fast and circulate as body provides energy;
7, during bean pulp fermentation, abundant lactic acid, acetic acid and glutamic acid after fermentation lactic acid content up to more than 3%, containing a large amount of lactic acid bacterias in fermented bean dregs, saccharomycete, acetic acid bacteria, the multiple probio such as actinomyces, total bacteria count content reaches more than 108cfu/g, a large amount of active probiotics enhances beneficial microorganism growth and breeding, inhibit Escherichia coli, the growth of the harmful bacterias such as salmonella, maintain the microecological balance of enteron aisle, improve the institutional framework of enteron aisle, ensure that the health of enteron aisle is unobstructed, enhance the immunologic function of feeding animals, improve the production performance of animal, prevent and reduce developing of disease,
8, improve the immunologic function sending out animal body, reduce antibiotic consumption or Substitute For Partial antibiotic, there is the double action of nutrition and raising immunity.
Existing fermented bean dregs in process of production, usually there is the phenomenon of unstable product quality, even the fermented bean dregs of same producer different batches, quality also differs greatly, trace it to its cause, except the factor that different manufacturers adopts different strain, different process, cause that fermented bean dregs difference in quality is large, another main cause of poor stability is: large scale fermentation is not easy to operate, material skewness, poor stability.Chinese invention patent CN103859150A discloses a kind of production technology of aspergillus oryzae fermented bean dregs, realizes the object of suitability for industrialized production mainly through the solid state rheology (triangular flask cultivation) to aspergillus oryzae; Aspergillus oryzae needs preactivated, complex steps, and in control fermentation microbiological contamination and ensureing that batch mixing is even, less effective.Chinese invention patent CN103859149A discloses a kind of production technology of lactobacillus-fermented dregs of beans, realizes the object of suitability for industrialized production mainly through the cultivation (serum bottle cultivation) to lactic acid bacteria; Lactic acid bacteria needs preactivated equally, lactic acid bacteria main decomposition carbohydrate, belongs to probio, is easy to microbiological contamination during the fermentation, and effect is also undesirable in control batch mixing is even.Chinese invention patent CN101558818A discloses a kind of production technology of hygrometric state solidly fermented and enzymatically hydrolyzed soybean meal, although for microbiological contamination problem, add the step that 0.3% acetic acid suppresses miscellaneous bacteria, but acetic acid is perishable on the one hand, add the safety risks of operating personnel, on the other hand, acetic acid, while suppression miscellaneous bacteria, also inhibits the growth of beneficial bacterium; Following tiling batch mixing ferments in fermentation vat, and cannot ensure for the even aspect of batch mixing, technique is stable not.To sum up, lack a kind of mature and stable in existing technique, can miscellaneous bacteria be suppressed and batch mixing is uniform, the solid state fermentation production technology of industrialization large scale fermentation dregs of beans can be realized.
Summary of the invention
The problem of extensive solid-state fermentation process stability in prior art, the invention provides a kind of fermented bean dregs production technology of stable applicable large-scale production, utilize method of the present invention can overcome the defect of existing operation difficulty, material skewness, product stability difference.By the finished product that technique of the present invention is made, without antigen and ANFs, solve the trophism diarrhea problem of animal, the a large amount of beneficial bacteriums contained maintain the microecological balance of enteron aisle, ensure that the health of enteron aisle, enhance the immunologic function of feeding animals, improve the production performance of animal, the generation of prevention and minimizing disease.
For achieving the above object, the technical solution adopted in the present invention is as follows:
A stable fermented bean dregs production technology for applicable large-scale production, comprises the following steps:
Step one, seed culture medium
The making of seed culture medium: mixed by the raw material of seed culture medium, put into culture medium, culture medium liquid amount is 1/2-2/3, and Rhizopus a rrhiz is accessed seed culture medium and cultivates, OD by high-temperature sterilization
700time cell concentration reach 20-30, make seed culture medium;
Step 2, fermentation medium
The making of fermentation medium: the raw material of fermentation medium is mixed, adds water, mix; High-temperature sterilization, then naturally cools, makes fermentation medium;
Step 3, fermented and cultured
Nutrient solution in seed culture medium in described step one is accessed the fermentation medium in described step 2, fermented and cultured; After fermentation, spray, stops fermentation after spray;
Step 4, drying and crushing
Fermented product in described step 3 is dried, pulverizes, sieve, obtain fermented bean dregs finished product.
Wherein, the raw material of described seed culture medium comprises: tryptone, glucose, yeast extract and sodium chloride.
Wherein, the material content of described seed culture medium is: tryptone 1.0-2.0g/L, glucose 2.0-4.0g/L, yeast extract 0.5-1.5g/L and sodium chloride 0.5-1.5g/L.
Wherein, the raw material of described fermentation medium comprises: dregs of beans, wheat bran, sucrose and betaine.
Wherein, the material content of described fermentation medium is: dregs of beans 60%-80%, wheat bran 10%-35%, sucrose 5%-10% and betaine 1%-2%.
Wherein, in described step one and described step 2, the temperature of high-temperature sterilization is 120-130 DEG C, preferably 121 DEG C.
Wherein, in described step one, the time of high-temperature sterilization is 15-30 minute, preferably 20 minutes.
Wherein, in described step 2, the time of high-temperature sterilization is 1-2 hour.
Wherein, in described step one, the concentration of described Rhizopus a rrhiz is 10
6-10
9individual/mL.
Wherein, in described step one, the inoculum concentration of described Rhizopus a rrhiz is 5%-10%.
Wherein, in described step one, the cultivation temperature of access seed culture medium is 30-35 DEG C, and incubation time is 15-20 hour.
Wherein, in described step 2, fermentation medium is in the thermostatic chamber of shower humidification, adopts shallow tray fermentation to carry out advantage cultivation.
Wherein, in described step 2, amount of water is that raw material and water ratio reach 1:(0.4-1.0), preferred 1:0.8.
Wherein, in described step 3, the inoculum concentration of seed culture medium is 5%-15%.
Wherein, in described step 3, the temperature of fermented and cultured is 25-30 DEG C; Fermented incubation time is 12-16 hour.
Wherein, in described step 3, add alkali solution of beet when described spray.
Wherein, in described step 3, the amount that spray adds described alkali solution of beet counts 0.1%-1% by fermentation medium dry weight.
Wherein, in described step 3, the concentration of described alkali solution of beet is 10%-30%.
Wherein, in described step 3, described alkali solution of beet have passed through micro-filtration bacteria removing.
Wherein, in described step 3, described spray time is 20-30 hour.
Wherein, in described step 4, described bake out temperature is 70 DEG C.
Wherein, in described step 4, described sieve aperture is 1.2mm.
Wherein, described betaine is a kind of in betaine HCL, sulphonic acid betaine or phosphoric acid betaine or at least containing wherein a kind of mixture.
Wherein, described microorganism OD value is an index of reflection thalli growth state, and OD is the abbreviation of opticaldensity (optical density), represents the optical density that detected material sponges.Usual 400 ~ 700nm is the scope of microbioassay, needs ultraviolet specrophotometer to survey maximum absorption wavelength.OD
700represent the OD value under ultraviolet 700nm.
Wherein, described Rhizopus a rrhiz, Latin is called Rhizopusarrizus, and for Mucoales Mucoraceae Rhizopus arrhizus belongs to, culture medium optimum cultivation temperature is 28-30 DEG C.Be mainly used in food processing.
Wherein, described betaine, English name: Betaine, another name is Glycinebetaine, Glycocollbetaine, Lycine, Oxyneuyine, betanidin, betaine, ethanoic acid hydroxide inner salt, be a kind of alkaloid found from beet, its molecular formula is C
5h
11nO2, molecular weight is 117.15, the squamous of the easy moisture absorption or prismatic crystallization, and heat is to about 310 DEG C decomposition, and taste is sweet.Very easily water-soluble, be soluble in methyl alcohol, be dissolved in ethanol, be insoluble in ether.Through the decomposition reaction of dense potassium hydroxide solution, can trimethylamine be generated, there is hygroscopicity, very easily deliquescence, and discharge trimethylamine.High temperature resistant.For antitumor, hypotensive anti-peptic ulcer and gastrointestinal dysfunction, treatment liver diseases.
Wherein, described betaine HCL, Chinese: betaine HCL; Chinese another name: betaine, betaine hydrochloride; English name: Betainehydrochloride, English another name: 1-Carboxy-N, N, N-trimethylmethanaminiumchloride; BetaineHCl; BETAINECHLORIDE molecular formula: C
5h
12clNO
2, molecular weight: 153.6, fusing point 241-242 DEG C, water-soluble 64.7g/100mL (25 DEG C).As food, feed addictive, pharmaceutical grade is used for gastrointestinal function conditioning agent.
Wherein, described sulfobetaines (SB) also can be called ammonium alkane sulfo group lactone, it is similar to alkyl betaine is trialkyl ammonium inner salt compound, just instead of the alkyl carboxylic acid in carboxybetaine with alkyl sulfonic acid, therefore is referred to as (alkyl) sulfobetaines.The tertiary amine of the side chain of the tertiary amine that sulfobetaines is derived by natural oil and chemical synthesis and chlorosulfonic acid react obtained.Because the difference of raw material can according to the adjustment of corresponding technique, the difference of reaction condition and catalyst, can obtain corresponding sulfobetaines.Molecular formula general formula: CH
3(CH
2)
nn+ (CH
3)
2(CH)
msO
3, n=7-17, m>=2 in formula.Sulfobetaines is because the negative electricity center in molecular structure is by highly acid sulfonate functional groups load, alkaline quaternary ammonium ion balances with the sulfonic group Ion Phase with same strong acid, thus they are different from carboxybetaine, inner salt can not be formed, can other all surface active ingredient, higher than corresponding carboxylic acid betaine fusing point, in hard water calcium soap is dispersed better.
Wherein, described phosphoric acid betaine, English Betainephosphate by name, molecular formula is C
5h
14nO
6p, molecular weight is 215.14.White is to micro-yellow crystalline powder, and odorlessness, taste is micro-puckery.Tool moisture absorption, very easily water-soluble, be dissolved in ethanol.
Compared to prior art, advantage of the present invention and beneficial effect as follows:
(1) fermentation strain has been carried out seed and expanded cultivation, decreased antagonism in the bacterial strain due to the uneven initiation of spore germination, achieve the stability of technique;
(2) in bean pulp fermentation, add betaine, material surface interfacial tension can be reduced, increase material mixture homogeneity; In addition, betaine can regulate the permeability of somatic cells film, avoids the thalli growth restriction because dissolved oxygen causes;
(3) Rhizopus arrhizus single strain fermented bean dregs is adopted to greatly reduce the probability of fermentation microbiological contamination, and can effectively eliminate ANFs in dregs of beans and reach good PD effect, but its palatability, phagostimulating effect are slightly poor, adding of betaine effectively overcomes this defect, contributes to the production of fermented bean dregs of high quality and at a reasonable price.
Figure of description
Fig. 1 schematically illustrates the schematic flow sheet of production technology of the present invention.
Detailed description of the invention
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in detail.Should be understood that, detailed description of the invention described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
The present invention is used for the fermented bean dregs production technology being applicable to large-scale production, as shown in Figure 1, comprises;
Step one, seed culture matrix manufacturing: tryptone 1.0-2.0g/L, glucose 2.0-4.0g/L, yeast extract 0.5-1.5g/L and sodium chloride 0.5-1.5g/L are mixed, culture medium liquid amount is 1/2-2/3, high-temperature sterilization, accesses seed culture medium by Rhizopus a rrhiz and cultivates, OD
700time cell concentration reach 20-30;
The making of step 2, fermentation medium: dregs of beans 60%-80%, wheat bran 10%-35%, sucrose 5%-10% and betaine 1%-2% are mixed, adds water, mix; High-temperature sterilization, then cools naturally;
Step 3, fermented and cultured: the nutrient solution in the seed culture medium in described step one is accessed the fermentation medium in described step 2, fermented and cultured; After fermentation, spray adds alkali solution of beet, after spray, stops fermentation;
Step 4, drying and crushing: dried by the fermented product in described step 3, pulverize, sieve, obtain fermented bean dregs finished product.
Wherein, in described step one and described step 2, the temperature of high-temperature sterilization is 121 DEG C.
Wherein, in described step one, the time of high-temperature sterilization is 20 minutes.
Wherein, in described step 2, the time of high-temperature sterilization is 1-2 hour, preferably 1.5 hours.
Wherein, in described step one, the concentration of described Rhizopus a rrhiz is 10
6-10
9individual/ml.
Wherein, in described step one, the inoculum concentration of described Rhizopus a rrhiz is 5%-10%, preferably 7%.
Wherein, in described step one, the cultivation temperature of access seed culture medium is 30-35 DEG C, and incubation time is 15-20 hour, preferable temperature 33 DEG C, 18 hours preferred time.
Wherein, in described step 2, fermentation medium is in the thermostatic chamber of shower humidification, adopts shallow tray fermentation to carry out advantage cultivation.
Wherein, in described step 2, amount of water is that raw material and water ratio reach 1:(0.4-1.0), preferred 1:0.8.
Wherein, in described step 3, the inoculum concentration of seed culture medium is 5%-15%, preferably 10%.
Wherein, in described step 3, the temperature of fermented and cultured is 25-30 DEG C; Fermented incubation time is 12-16 hour, preferably 27 DEG C, preferably 15 hours.
Wherein, in described step 3, the amount that spray adds described alkali solution of beet counts 0.1%-1% by fermentation medium dry weight, and preferably 0.7%.
Wherein, in described step 3, the concentration of described alkali solution of beet is 10%-30%, preferably 15%.
Wherein, in described step 3, described alkali solution of beet have passed through micro-filtration bacteria removing.
Wherein, in described step 3, described spray time is 20-30 hour, preferably 24 hours.
Wherein, in described step 4, described bake out temperature is 70 DEG C.
Wherein, in described step 4, described sieve aperture is 1.2mm.
Wherein, described betaine is a kind of in betaine HCL, sulphonic acid betaine or phosphoric acid betaine or at least containing wherein a kind of mixture.
Below by specific embodiment, illustrate embodiments of the present invention:
Embodiment 1
The preparation of seed liquor in step one, seed culture medium: tryptone 1.5g, glucose 3.0g, yeast extract 1.5g, sodium chloride 0.5g, water 100mL, culture medium liquid amount is 100mL/500mL triangular flask, after 121 DEG C of sterilizing 20min, is 10 by concentration
8the Rhizopus a rrhiz of individual/mL according to 5% inoculum concentration access seed culture medium, cultivate 20 hours at 30 DEG C, cell concentration reaches 25;
Prepared by step 2, fermentation medium: dregs of beans 70%, wheat bran 24%, sucrose 5%, and betaine HCL 1%, adds water and make material-water ratio reach 0.8, mix; 121 DEG C of high-temperature sterilizations 1 hour afterwards, cool naturally;
Step 3, fermented and cultured: by seed liquor in step one by the inoculum concentration access fermentation medium of 5%, carry out fermented and cultured with under 30 DEG C of conditions; Ferment to after 16 hours, spray adds 0.5% through the degerming betaine HCL solution of micro-filtration, and solution concentration is stop fermentation after 10%, 30 hours.
Step 4, fermented product were pulverized 1.2mm and were sieved and obtained fermented bean dregs finished product after drying through 60 DEG C.
In the present embodiment, fermentation strain is carried out seed and expanded cultivation, decreased antagonism in the bacterial strain due to the uneven initiation of spore germination, achieve the stability of technique;
Utilize the method for the present embodiment can overcome the defect of existing operation difficulty, material skewness, product stability difference.By the finished product that technique of the present invention is made, without antigen and ANFs, solve the trophism diarrhea problem of animal, the a large amount of beneficial bacteriums contained maintain the microecological balance of enteron aisle, ensure that the health of enteron aisle, enhance the immunologic function of feeding animals, improve the production performance of animal, the generation of prevention and minimizing disease.
Embodiment 2
The present embodiment is as different from Example 1: step one is omitted, directly by 10
8the Rhizopus a rrhiz of individual/mL according to 5% inoculum concentration access fermentation medium.
In the present embodiment, Rhizopus arrhizus single strain fermented bean dregs is adopted to greatly reduce the probability of fermentation microbiological contamination, and effectively can eliminate these ANFs such as trypsin ihhibitor in dregs of beans, phytic acid, soybean lectin, urase, compound sugar, lipoxidase, Soybean antigen protein (sensitizing factor) and goitrin and reach good PD effect, the content of small molecular protein and little peptide accounts for more than 70% of total protein, promote little peptide and with it in conjunction with the absorption of mineral element, promote growth of animal.
Embodiment 3
The present embodiment is as different from Example 1: in step 3, spray adds the amount of betaine HCL is 1%, and other are identical with embodiment 1.
In the present embodiment, compared with embodiment 1, expanding the consumption of betaine, is based on following reason: in bean pulp fermentation, add the pleasantly sweet betaine of band, can reduce material surface interfacial tension, increase material mixture homogeneity; In addition, betaine can regulate the permeability of somatic cells film, avoids the thalli growth restriction because dissolved oxygen causes;
And, although the Rhizopus arrhizus single strain fermented bean dregs adopted greatly reduces the probability of fermentation microbiological contamination, and can effectively eliminate ANFs in dregs of beans and reach good PD effect, but its palatability, phagostimulating effect are slightly poor, adding of betaine (sweetener) effectively overcomes this defect, contributes to the production of fermented bean dregs of high quality and at a reasonable price.
Embodiment 4
The present embodiment is as different from Example 1: the betaine composition in step 2, step 3 removes, and other are identical with embodiment 1.
In the present embodiment, fermentation strain is carried out seed and expanded cultivation, decreased antagonism in the bacterial strain due to the uneven initiation of spore germination, achieve the stability of technique; Adopt Rhizopus arrhizus single strain fermented bean dregs to greatly reduce the probability of fermentation microbiological contamination, and can effectively eliminate ANFs in dregs of beans and reach good PD effect.
Embodiment 5
The present embodiment is as different from Example 1: the betaine in step 2, step 3 is changed to phosphoric acid betaine, and other are identical with embodiment 1.
In the present embodiment, adopting phosphorylation betaine, is based on phosphorylation betaine odorlessness, overcomes and introduces other smells and the problem that affects dregs of beans fragrance originally, improve palatability, attractant is better, improves feed local flavor, increases animal appetite, Long-Time Service forms hobby, improve animal feed intake, growth promoting effects, increase yield.
Embodiment 6
The present embodiment is as different from Example 1: the betaine in step 2, step 3 is changed to sulphonic acid betaine, and other are identical with embodiment 1.
In the present embodiment, adopt sulfonated betaine, because the negative electricity center in molecular structure is by highly acid sulfonate functional groups load based on sulfobetaines, alkaline quaternary ammonium ion balances with the sulfonic group Ion Phase with same strong acid, thus they are different from carboxybetaine, can not inner salt be formed, can other all surface active ingredient, higher than corresponding carboxylic acid betaine fusing point, in hard water calcium soap is dispersed better.
In above-described embodiment, the indexs such as fermented bean dregs color, smell, viscosity, PD degree are monitored.Embodiment 1,2 the results are shown in Table 1, and embodiment 1,3 the results are shown in Table 2, and embodiment 1,4 the results are shown in Table 3, and embodiment 4,5 the results are shown in Table 4, and embodiment 4,6 the results are shown in Table for 5 (in following table, "+" represents high-quality degree).
Table 1, seed liquor effect test
| Embodiment | Color | Smell | Viscosity | PD degree |
| Embodiment 1 | ++++ | ++++ | +++ | Degraded thoroughly |
| Embodiment 2 | ++ | +++ | ++ | Degrade not thorough |
Table 2, betaine addition are on the impact of test
| Embodiment | Color | Smell | Viscosity | PD degree |
| Embodiment 1 | ++++ | ++++ | +++ | Degraded thoroughly |
| Embodiment 3 | ++++ | ++++ | ++ | Degraded thoroughly |
Table 3, hydrochloride betaine are on the impact of ferment effect
| Embodiment | Color | Smell | Viscosity | PD degree |
| Embodiment 1 | ++++ | ++++ | +++ | Degraded thoroughly |
| Embodiment 4 | ++ | ++ | ++ | Degrade not thorough |
Table 4, phosphate betaine are on the impact of ferment effect
| Embodiment 4 | ++ | ++ | ++ | Degrade not thorough |
| Embodiment 5 | +++ | ++++ | +++ | Degraded thoroughly |
Table 5, sulphonic acid betaine are on the impact of ferment effect
| Embodiment 4 | ++ | ++ | ++ | Degrade not thorough |
| Embodiment 6 | ++++ | +++ | ++ | Degraded thoroughly |
Claims (10)
1. a fermented bean dregs production technology for applicable large-scale production, is characterized in that;
Step one, seed culture medium
The making of seed culture medium: mixed by the raw material of seed culture medium, put into culture medium, culture medium liquid amount is 1/2-2/3, and Rhizopus a rrhiz is accessed seed culture medium and cultivates, OD by high-temperature sterilization
700time cell concentration reach 20-30, make seed culture medium;
Step 2, fermentation medium
The making of fermentation medium: the raw material of fermentation medium is mixed, adds water, mix; High-temperature sterilization, then naturally cools, makes fermentation medium;
Step 3, fermented and cultured
Nutrient solution in seed culture medium in described step one is accessed the fermentation medium in described step 2, fermented and cultured; After fermentation, spray, stops fermentation after spray;
Step 4, drying and crushing
Fermented product in described step 3 is dried, pulverizes, sieve, obtain fermented bean dregs finished product.
2. the fermented bean dregs production technology of applicable large-scale production according to claim 1, is characterized in that: the raw material of described seed culture medium comprises: tryptone 1.0-2.0g/L, glucose 2.0-4.0g/L, yeast extract 0.5-1.5g/L and sodium chloride 0.5-1.5g/L.
3. the fermented bean dregs production technology of applicable large-scale production according to claim 1, is characterized in that: the raw material of described fermentation medium comprises: dregs of beans 60%-80%, wheat bran 10%-35%, sucrose 5%-10% and betaine 1%-2%.
4. the fermented bean dregs production technology of applicable large-scale production according to claim 1, is characterized in that: in described step one and described step 2, the temperature of high-temperature sterilization is 120-130 DEG C; In described step one, the time of high-temperature sterilization is 15-30 minute; In described step 2, the time of high-temperature sterilization is 1-2 hour.
5. the fermented bean dregs production technology of applicable large-scale production according to claim 1, is characterized in that: in described step one, and the concentration of described Rhizopus a rrhiz is 10
6-10
9individual/ml; The inoculum concentration of described Rhizopus a rrhiz is 5%-10%.
6. the fermented bean dregs production technology of applicable large-scale production according to claim 1, is characterized in that: in described step 2, fermentation medium is in the thermostatic chamber of shower humidification, adopts shallow tray fermentation to carry out advantage cultivation; Amount of water is that raw material and water ratio reach 1:(0.4-1.0).
7. the fermented bean dregs production technology of applicable large-scale production according to claim 1, is characterized in that: in described step 3, and the inoculum concentration of seed culture medium is 5%-15%; The temperature of fermented and cultured is 25-30 DEG C; Fermented incubation time is 12-16 hour.
8. the fermented bean dregs production technology of applicable large-scale production according to claim 1, is characterized in that: in described step 3, adds alkali solution of beet when described spray; The amount that spray adds described alkali solution of beet counts 0.1%-1% by fermentation medium dry weight; The concentration of described alkali solution of beet is 10%-30%; Described alkali solution of beet have passed through micro-filtration bacteria removing; Described spray time is 20-30 hour.
9. the fermented bean dregs production technology of applicable large-scale production according to claim 1, is characterized in that: in described step 4, described bake out temperature is 70 DEG C; Described sieve aperture is 1.2mm.
10. the fermented bean dregs production technology of applicable large-scale production according to claim 1, is characterized in that: described betaine is a kind of in betaine HCL, sulphonic acid betaine or phosphoric acid betaine or at least containing wherein a kind of mixture.
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| CN108902545A (en) * | 2018-06-20 | 2018-11-30 | 西南大学 | A kind of Eriocheir sinensis fattening feed that biofermentation is modified |
| CN108902548A (en) * | 2018-06-21 | 2018-11-30 | 西南大学 | A kind of fish culture composite bait handled by solid state fermentation |
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| CN108902548A (en) * | 2018-06-21 | 2018-11-30 | 西南大学 | A kind of fish culture composite bait handled by solid state fermentation |
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Application publication date: 20151209 |