CN104186918A - Preparing method of corn gluten polypeptide for fodder - Google Patents
Preparing method of corn gluten polypeptide for fodder Download PDFInfo
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- CN104186918A CN104186918A CN201410363715.3A CN201410363715A CN104186918A CN 104186918 A CN104186918 A CN 104186918A CN 201410363715 A CN201410363715 A CN 201410363715A CN 104186918 A CN104186918 A CN 104186918A
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- fodder
- corn gluten
- polypeptide
- clear liquid
- aspergillus sojae
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Abstract
The invention relates to a preparing method of a corn gluten polypeptide for fodder, belongs to the technical field of processing of fodder and particularly relates to a method of preparing the corn gluten polypeptide for the fodder by taking corn gluten meal as a raw material. The method comprises the following steps: swelling the corn gluten meal, then adding water, uniformly stirring, adjusting pH to 7.0-7.2, sterilizing, cooling to 45-50 DEG C, adding a neutral protease, performing hydrolysis for 6-8h, boiling, cooling to 26-28 DEG C, inoculating aspergillus sojae, fermenting for 26-30h, centrifuging to obtain clear liquid, concentrating the clear liquid and spray-drying to obtain a finished product. The preparing method of the corn gluten polypeptide for the fodder, disclosed by the invention, effectively integrates the respective advantages of a swelling method, an enzyme method and a fermentation method; the corn gluten polypeptide for the fodder is rich in nutrients and has prominent effects; furthermore, the prepared gluten polypeptide has no bitter taste, no peculiar smell and good palatability.
Description
Technical field
The invention belongs to feed processing technology field, particularly be the method for utilizing corn protein powder to make fodder maize polypeptide for raw material.
Background technology
Corn protein powder is the byproduct of corn kernel after producing starch, its proteinaceous nutrient composition is abundant, and there is special taste and color and luster, can be used as feed and use, with conventional fish meal, the soya-bean cake comparison of feed industry, resources advantage is obvious, feeding value is high,, containing poisonous and harmful substance, do not need to process again, can directly be used as protein raw materials.Although corn protein powder protein content is high, but insoluble protein is wherein difficult for being digested and assimilated by animal, because insoluble protein easily and other larger molecular organicses or micro-combination in animal intestines and stomach, part insoluble protein does not also have digested absorption just by animal, to be excreted, become the composition that forms argol material, cause great waste, also polluted environment simultaneously.
If utilize bionic method, zein is degraded into zein polypeptide, zein polypeptide not only can overcome the soluble and not non-digestible shortcoming of zein, can also increase many physiological functions, because can improve the immunity of animal, effectively prevent the generation of various viral diseases, improve cultivation survival rate, due to corn peptide, there is in addition the fragrance of fermentation, so good palatability, can improve animal feed intake, promote growth of animal.
Summary of the invention
The object of the present invention is to provide a kind of method of corn protein powder as the bionic method making of prepared using fodder maize polypeptide of take.
The object of the present invention is achieved like this:
Corn protein powder is expanded, take the popcorn albumen powder of certain mass, the water that adds 12-18 times of quality, stir, PH is adjusted to 7.0-7.2, 121 ℃ of sterilizing 20-30 minute, cool to 45-50 ℃, in every gram of raw material, add the ratio of 80-100 unit to add neutral proteinase, at 45-50 ℃ of Water Under solution 6-8 hour, then boil 10-15 minute, cool to 26-28 ℃, press the inoculum concentration inoculation Aspergillus sojae seed liquor of 5-10%, at 150-180rpm, the shaking table top fermentation 26-30 hour of 26-28 ℃, the centrifugal clear liquid that obtains, clear liquid is again through concentrated, spraying is drying to obtain finished product.
The present invention also comprises following feature:
The preparation method of described Aspergillus sojae seed liquor is: get 5-7B
xbrewer's wort, 121 ℃ of sterilizing 15-20 minute, are cooled to 26-28 ℃, and inoculation Aspergillus sojae is cultivated 18-20 hour on the shaking table of 150-180rpm, 26-28 ℃.
Zein polypeptide of the present invention is to take corn protein powder as raw material, successively by expanded, enzymolysis and fermentation, the macromolecular substances such as protein in corn protein powder are degraded into the small-molecule substances such as polypeptide, effectively combine expanded method, enzyme process and fermentation method separately advantage, not only nutritious, effect is given prominence to, and the polypeptide making is without bitter taste, free from extraneous odour, good palatability.
Specific embodiments
The bacterial strain Aspergillus sojae CICC40357 that the present invention adopts purchases in Chinese industrial microorganism fungus kind preservation administrative center.
Get 6B
xbrewer's wort, 121 ℃ of sterilizings 20 minutes, are cooled to 28 ℃, and inoculation Aspergillus sojae is cultivated and within 20 hours, is obtained Aspergillus sojae seed liquor on 160rpm, the shaking table of 28 ℃.
Corn protein powder is expanded, take the popcorn albumen powder of certain mass, the water that adds 15 times of quality, stir, PH is adjusted to 7.0,121 ℃ of sterilizings 20 minutes, cool to 50 ℃, in every gram of raw material, add the ratio of 80 units to add neutral proteinase, 50 ℃ of Water Under solutions 6 hours, then boil 15 minutes, cool to 28 ℃, by 10% inoculum concentration inoculation Aspergillus sojae seed liquor, in 160rpm, the shaking table top fermentation 30 hours of 28 ℃, the centrifugal clear liquid that obtains, clear liquid is drying to obtain finished product through concentrated, spraying again.
Claims (1)
1. the preparation method of fodder maize polypeptide, is characterized in that being prepared from through following process:
(1) get 5-7B
xbrewer's wort, 121 ℃ of sterilizing 15-20 minute, are cooled to 26-28 ℃, and inoculation Aspergillus sojae is cultivated and within 18-20 hour, is obtained Aspergillus sojae seed liquor on the shaking table of 150-180rpm, 26-28 ℃;
(2) corn protein powder is expanded, take the popcorn albumen powder of certain mass, the water that adds 12-18 times of quality, stir, PH is adjusted to 7.0-7.2, 121 ℃ of sterilizing 20-30 minute, cool to 45-50 ℃, in every gram of raw material, add the ratio of 80-100 unit to add neutral proteinase, at 45-50 ℃ of Water Under solution 6-8 hour, then boil 10-15 minute, cool to 26-28 ℃, press the inoculum concentration inoculation Aspergillus sojae seed liquor of 5-10%, at 150-180rpm, the shaking table top fermentation 26-30 hour of 26-28 ℃, the centrifugal clear liquid that obtains, clear liquid is again through concentrated, spraying is drying to obtain finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410363715.3A CN104186918A (en) | 2014-07-29 | 2014-07-29 | Preparing method of corn gluten polypeptide for fodder |
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| CN201410363715.3A CN104186918A (en) | 2014-07-29 | 2014-07-29 | Preparing method of corn gluten polypeptide for fodder |
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| CN104186918A true CN104186918A (en) | 2014-12-10 |
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| CN201410363715.3A Pending CN104186918A (en) | 2014-07-29 | 2014-07-29 | Preparing method of corn gluten polypeptide for fodder |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105533209A (en) * | 2016-01-07 | 2016-05-04 | 哈尔滨伟平科技开发有限公司 | Method for preparing pig feed additive |
| CN105595022A (en) * | 2016-01-07 | 2016-05-25 | 哈尔滨伟平科技开发有限公司 | Making method of dairy cow feed additives |
| CN105639142A (en) * | 2016-01-07 | 2016-06-08 | 哈尔滨伟平科技开发有限公司 | Production method of pig feed additive |
| CN105795110A (en) * | 2016-04-18 | 2016-07-27 | 哈尔滨伟平科技开发有限公司 | Preparation method of egg-chicken feed additive |
| CN105831474A (en) * | 2016-04-18 | 2016-08-10 | 哈尔滨伟平科技开发有限公司 | Preparation method of laying duck feed additive |
| CN105831473A (en) * | 2016-04-18 | 2016-08-10 | 哈尔滨伟平科技开发有限公司 | Method for preparing chicken feed additive |
| CN105851481A (en) * | 2016-05-29 | 2016-08-17 | 哈尔滨伟平科技开发有限公司 | Preparation method of milk cow feed additive |
| CN105876235A (en) * | 2016-04-18 | 2016-08-24 | 哈尔滨伟平科技开发有限公司 | Manufacturing method of feed additive for egg laying ducks |
| CN105876198A (en) * | 2016-04-18 | 2016-08-24 | 哈尔滨伟平科技开发有限公司 | Preparation method of pig feed additive |
| CN105941984A (en) * | 2016-05-29 | 2016-09-21 | 哈尔滨伟平科技开发有限公司 | Preparation method of laying hen feed additive |
| CN105995079A (en) * | 2016-05-29 | 2016-10-12 | 哈尔滨伟平科技开发有限公司 | Preparation method of pig feed additive |
| CN105994942A (en) * | 2016-05-29 | 2016-10-12 | 哈尔滨伟平科技开发有限公司 | Preparation method of feed additive for laying ducks |
| CN106509363A (en) * | 2017-01-07 | 2017-03-22 | 哈尔滨伟平科技开发有限公司 | Making method of high-protein fermented feed |
| WO2023017710A1 (en) * | 2021-08-13 | 2023-02-16 | 株式会社フジワラテクノアート | Filamentous fungi solid culture, composition, food, animal feedstuff, food additive, or animal feedstuff additive, and method for producing filamentous fungi solid culture |
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| JP2000253805A (en) * | 1999-03-10 | 2000-09-19 | Mie Prefecture | Peptide derived from maize protein and bread dough |
| CN101126103A (en) * | 2007-08-17 | 2008-02-20 | 黑龙江省宏达生物工程有限公司 | Method for preparing corn protein peptide with high F valve by microorganism zymotechnics |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105595022A (en) * | 2016-01-07 | 2016-05-25 | 哈尔滨伟平科技开发有限公司 | Making method of dairy cow feed additives |
| CN105639142A (en) * | 2016-01-07 | 2016-06-08 | 哈尔滨伟平科技开发有限公司 | Production method of pig feed additive |
| CN105533209A (en) * | 2016-01-07 | 2016-05-04 | 哈尔滨伟平科技开发有限公司 | Method for preparing pig feed additive |
| CN105876198A (en) * | 2016-04-18 | 2016-08-24 | 哈尔滨伟平科技开发有限公司 | Preparation method of pig feed additive |
| CN105795110A (en) * | 2016-04-18 | 2016-07-27 | 哈尔滨伟平科技开发有限公司 | Preparation method of egg-chicken feed additive |
| CN105831474A (en) * | 2016-04-18 | 2016-08-10 | 哈尔滨伟平科技开发有限公司 | Preparation method of laying duck feed additive |
| CN105831473A (en) * | 2016-04-18 | 2016-08-10 | 哈尔滨伟平科技开发有限公司 | Method for preparing chicken feed additive |
| CN105876235A (en) * | 2016-04-18 | 2016-08-24 | 哈尔滨伟平科技开发有限公司 | Manufacturing method of feed additive for egg laying ducks |
| CN105851481A (en) * | 2016-05-29 | 2016-08-17 | 哈尔滨伟平科技开发有限公司 | Preparation method of milk cow feed additive |
| CN105941984A (en) * | 2016-05-29 | 2016-09-21 | 哈尔滨伟平科技开发有限公司 | Preparation method of laying hen feed additive |
| CN105995079A (en) * | 2016-05-29 | 2016-10-12 | 哈尔滨伟平科技开发有限公司 | Preparation method of pig feed additive |
| CN105994942A (en) * | 2016-05-29 | 2016-10-12 | 哈尔滨伟平科技开发有限公司 | Preparation method of feed additive for laying ducks |
| CN106509363A (en) * | 2017-01-07 | 2017-03-22 | 哈尔滨伟平科技开发有限公司 | Making method of high-protein fermented feed |
| WO2023017710A1 (en) * | 2021-08-13 | 2023-02-16 | 株式会社フジワラテクノアート | Filamentous fungi solid culture, composition, food, animal feedstuff, food additive, or animal feedstuff additive, and method for producing filamentous fungi solid culture |
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Application publication date: 20141210 |