CN104186918A - Preparing method of corn gluten polypeptide for fodder - Google Patents

Preparing method of corn gluten polypeptide for fodder Download PDF

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Publication number
CN104186918A
CN104186918A CN201410363715.3A CN201410363715A CN104186918A CN 104186918 A CN104186918 A CN 104186918A CN 201410363715 A CN201410363715 A CN 201410363715A CN 104186918 A CN104186918 A CN 104186918A
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CN
China
Prior art keywords
fodder
corn gluten
polypeptide
preparing
clear liquid
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Pending
Application number
CN201410363715.3A
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Chinese (zh)
Inventor
吕庆茂
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HARBIN WEIPING TECHNOLOGY DEVELOPMENT Co Ltd
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HARBIN WEIPING TECHNOLOGY DEVELOPMENT Co Ltd
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Application filed by HARBIN WEIPING TECHNOLOGY DEVELOPMENT Co Ltd filed Critical HARBIN WEIPING TECHNOLOGY DEVELOPMENT Co Ltd
Priority to CN201410363715.3A priority Critical patent/CN104186918A/en
Publication of CN104186918A publication Critical patent/CN104186918A/en
Pending legal-status Critical Current

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Abstract

The invention relates to a preparing method of a corn gluten polypeptide for fodder, belongs to the technical field of processing of fodder and particularly relates to a method of preparing the corn gluten polypeptide for the fodder by taking corn gluten meal as a raw material. The method comprises the following steps: swelling the corn gluten meal, then adding water, uniformly stirring, adjusting pH to 7.0-7.2, sterilizing, cooling to 45-50 DEG C, adding a neutral protease, performing hydrolysis for 6-8h, boiling, cooling to 26-28 DEG C, inoculating aspergillus sojae, fermenting for 26-30h, centrifuging to obtain clear liquid, concentrating the clear liquid and spray-drying to obtain a finished product. The preparing method of the corn gluten polypeptide for the fodder, disclosed by the invention, effectively integrates the respective advantages of a swelling method, an enzyme method and a fermentation method; the corn gluten polypeptide for the fodder is rich in nutrients and has prominent effects; furthermore, the prepared gluten polypeptide has no bitter taste, no peculiar smell and good palatability.

Description

The preparation method of fodder maize polypeptide
Technical field
The invention belongs to feed processing technology field, particularly be the method for utilizing corn protein powder to make fodder maize polypeptide for raw material.
Background technology
Corn protein powder is the byproduct of corn kernel after producing starch, its proteinaceous nutrient composition is abundant, and there is special taste and color and luster, can be used as feed and use, with conventional fish meal, the soya-bean cake comparison of feed industry, resources advantage is obvious, feeding value is high,, containing poisonous and harmful substance, do not need to process again, can directly be used as protein raw materials.Although corn protein powder protein content is high, but insoluble protein is wherein difficult for being digested and assimilated by animal, because insoluble protein easily and other larger molecular organicses or micro-combination in animal intestines and stomach, part insoluble protein does not also have digested absorption just by animal, to be excreted, become the composition that forms argol material, cause great waste, also polluted environment simultaneously.
If utilize bionic method, zein is degraded into zein polypeptide, zein polypeptide not only can overcome the soluble and not non-digestible shortcoming of zein, can also increase many physiological functions, because can improve the immunity of animal, effectively prevent the generation of various viral diseases, improve cultivation survival rate, due to corn peptide, there is in addition the fragrance of fermentation, so good palatability, can improve animal feed intake, promote growth of animal.
Summary of the invention
The object of the present invention is to provide a kind of method of corn protein powder as the bionic method making of prepared using fodder maize polypeptide of take.
The object of the present invention is achieved like this:
Corn protein powder is expanded, take the popcorn albumen powder of certain mass, the water that adds 12-18 times of quality, stir, PH is adjusted to 7.0-7.2, 121 ℃ of sterilizing 20-30 minute, cool to 45-50 ℃, in every gram of raw material, add the ratio of 80-100 unit to add neutral proteinase, at 45-50 ℃ of Water Under solution 6-8 hour, then boil 10-15 minute, cool to 26-28 ℃, press the inoculum concentration inoculation Aspergillus sojae seed liquor of 5-10%, at 150-180rpm, the shaking table top fermentation 26-30 hour of 26-28 ℃, the centrifugal clear liquid that obtains, clear liquid is again through concentrated, spraying is drying to obtain finished product.
The present invention also comprises following feature:
The preparation method of described Aspergillus sojae seed liquor is: get 5-7B xbrewer's wort, 121 ℃ of sterilizing 15-20 minute, are cooled to 26-28 ℃, and inoculation Aspergillus sojae is cultivated 18-20 hour on the shaking table of 150-180rpm, 26-28 ℃.
Zein polypeptide of the present invention is to take corn protein powder as raw material, successively by expanded, enzymolysis and fermentation, the macromolecular substances such as protein in corn protein powder are degraded into the small-molecule substances such as polypeptide, effectively combine expanded method, enzyme process and fermentation method separately advantage, not only nutritious, effect is given prominence to, and the polypeptide making is without bitter taste, free from extraneous odour, good palatability.
Specific embodiments
The bacterial strain Aspergillus sojae CICC40357 that the present invention adopts purchases in Chinese industrial microorganism fungus kind preservation administrative center.
Get 6B xbrewer's wort, 121 ℃ of sterilizings 20 minutes, are cooled to 28 ℃, and inoculation Aspergillus sojae is cultivated and within 20 hours, is obtained Aspergillus sojae seed liquor on 160rpm, the shaking table of 28 ℃.
Corn protein powder is expanded, take the popcorn albumen powder of certain mass, the water that adds 15 times of quality, stir, PH is adjusted to 7.0,121 ℃ of sterilizings 20 minutes, cool to 50 ℃, in every gram of raw material, add the ratio of 80 units to add neutral proteinase, 50 ℃ of Water Under solutions 6 hours, then boil 15 minutes, cool to 28 ℃, by 10% inoculum concentration inoculation Aspergillus sojae seed liquor, in 160rpm, the shaking table top fermentation 30 hours of 28 ℃, the centrifugal clear liquid that obtains, clear liquid is drying to obtain finished product through concentrated, spraying again.

Claims (1)

1. the preparation method of fodder maize polypeptide, is characterized in that being prepared from through following process:
(1) get 5-7B xbrewer's wort, 121 ℃ of sterilizing 15-20 minute, are cooled to 26-28 ℃, and inoculation Aspergillus sojae is cultivated and within 18-20 hour, is obtained Aspergillus sojae seed liquor on the shaking table of 150-180rpm, 26-28 ℃;
(2) corn protein powder is expanded, take the popcorn albumen powder of certain mass, the water that adds 12-18 times of quality, stir, PH is adjusted to 7.0-7.2, 121 ℃ of sterilizing 20-30 minute, cool to 45-50 ℃, in every gram of raw material, add the ratio of 80-100 unit to add neutral proteinase, at 45-50 ℃ of Water Under solution 6-8 hour, then boil 10-15 minute, cool to 26-28 ℃, press the inoculum concentration inoculation Aspergillus sojae seed liquor of 5-10%, at 150-180rpm, the shaking table top fermentation 26-30 hour of 26-28 ℃, the centrifugal clear liquid that obtains, clear liquid is again through concentrated, spraying is drying to obtain finished product.
CN201410363715.3A 2014-07-29 2014-07-29 Preparing method of corn gluten polypeptide for fodder Pending CN104186918A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
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Application Number Priority Date Filing Date Title
CN201410363715.3A CN104186918A (en) 2014-07-29 2014-07-29 Preparing method of corn gluten polypeptide for fodder

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CN104186918A true CN104186918A (en) 2014-12-10

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Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105533209A (en) * 2016-01-07 2016-05-04 哈尔滨伟平科技开发有限公司 Method for preparing pig feed additive
CN105595022A (en) * 2016-01-07 2016-05-25 哈尔滨伟平科技开发有限公司 Making method of dairy cow feed additives
CN105639142A (en) * 2016-01-07 2016-06-08 哈尔滨伟平科技开发有限公司 Production method of pig feed additive
CN105795110A (en) * 2016-04-18 2016-07-27 哈尔滨伟平科技开发有限公司 Preparation method of egg-chicken feed additive
CN105831474A (en) * 2016-04-18 2016-08-10 哈尔滨伟平科技开发有限公司 Preparation method of laying duck feed additive
CN105831473A (en) * 2016-04-18 2016-08-10 哈尔滨伟平科技开发有限公司 Method for preparing chicken feed additive
CN105851481A (en) * 2016-05-29 2016-08-17 哈尔滨伟平科技开发有限公司 Preparation method of milk cow feed additive
CN105876235A (en) * 2016-04-18 2016-08-24 哈尔滨伟平科技开发有限公司 Manufacturing method of feed additive for egg laying ducks
CN105876198A (en) * 2016-04-18 2016-08-24 哈尔滨伟平科技开发有限公司 Preparation method of pig feed additive
CN105941984A (en) * 2016-05-29 2016-09-21 哈尔滨伟平科技开发有限公司 Preparation method of laying hen feed additive
CN105995079A (en) * 2016-05-29 2016-10-12 哈尔滨伟平科技开发有限公司 Preparation method of pig feed additive
CN105994942A (en) * 2016-05-29 2016-10-12 哈尔滨伟平科技开发有限公司 Preparation method of feed additive for laying ducks
CN106509363A (en) * 2017-01-07 2017-03-22 哈尔滨伟平科技开发有限公司 Making method of high-protein fermented feed

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000253805A (en) * 1999-03-10 2000-09-19 Mie Prefecture Peptide derived from maize protein and bread dough
CN101126103A (en) * 2007-08-17 2008-02-20 黑龙江省宏达生物工程有限公司 Method for preparing corn protein peptide with high F valve by microorganism zymotechnics
CN101353685A (en) * 2008-09-05 2009-01-28 长春美能生物工程有限责任公司 Zein active peptide and preparation technique thereof
CN102321717A (en) * 2011-09-01 2012-01-18 山东中谷淀粉糖有限公司 Preparation method for corn bioactive peptide
CN102864201A (en) * 2012-10-09 2013-01-09 陕西同正科技有限公司 Preparation method of corn active peptides
CN103766609A (en) * 2014-02-13 2014-05-07 哈尔滨伟平科技开发有限公司 Preparation method of polypeptide feed additive

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000253805A (en) * 1999-03-10 2000-09-19 Mie Prefecture Peptide derived from maize protein and bread dough
CN101126103A (en) * 2007-08-17 2008-02-20 黑龙江省宏达生物工程有限公司 Method for preparing corn protein peptide with high F valve by microorganism zymotechnics
CN101353685A (en) * 2008-09-05 2009-01-28 长春美能生物工程有限责任公司 Zein active peptide and preparation technique thereof
CN102321717A (en) * 2011-09-01 2012-01-18 山东中谷淀粉糖有限公司 Preparation method for corn bioactive peptide
CN102864201A (en) * 2012-10-09 2013-01-09 陕西同正科技有限公司 Preparation method of corn active peptides
CN103766609A (en) * 2014-02-13 2014-05-07 哈尔滨伟平科技开发有限公司 Preparation method of polypeptide feed additive

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105533209A (en) * 2016-01-07 2016-05-04 哈尔滨伟平科技开发有限公司 Method for preparing pig feed additive
CN105595022A (en) * 2016-01-07 2016-05-25 哈尔滨伟平科技开发有限公司 Making method of dairy cow feed additives
CN105639142A (en) * 2016-01-07 2016-06-08 哈尔滨伟平科技开发有限公司 Production method of pig feed additive
CN105795110A (en) * 2016-04-18 2016-07-27 哈尔滨伟平科技开发有限公司 Preparation method of egg-chicken feed additive
CN105831474A (en) * 2016-04-18 2016-08-10 哈尔滨伟平科技开发有限公司 Preparation method of laying duck feed additive
CN105831473A (en) * 2016-04-18 2016-08-10 哈尔滨伟平科技开发有限公司 Method for preparing chicken feed additive
CN105876198A (en) * 2016-04-18 2016-08-24 哈尔滨伟平科技开发有限公司 Preparation method of pig feed additive
CN105876235A (en) * 2016-04-18 2016-08-24 哈尔滨伟平科技开发有限公司 Manufacturing method of feed additive for egg laying ducks
CN105851481A (en) * 2016-05-29 2016-08-17 哈尔滨伟平科技开发有限公司 Preparation method of milk cow feed additive
CN105941984A (en) * 2016-05-29 2016-09-21 哈尔滨伟平科技开发有限公司 Preparation method of laying hen feed additive
CN105995079A (en) * 2016-05-29 2016-10-12 哈尔滨伟平科技开发有限公司 Preparation method of pig feed additive
CN105994942A (en) * 2016-05-29 2016-10-12 哈尔滨伟平科技开发有限公司 Preparation method of feed additive for laying ducks
CN106509363A (en) * 2017-01-07 2017-03-22 哈尔滨伟平科技开发有限公司 Making method of high-protein fermented feed

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Application publication date: 20141210