CN102321717A - Preparation method for corn bioactive peptide - Google Patents
Preparation method for corn bioactive peptide Download PDFInfo
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- CN102321717A CN102321717A CN201110256591A CN201110256591A CN102321717A CN 102321717 A CN102321717 A CN 102321717A CN 201110256591 A CN201110256591 A CN 201110256591A CN 201110256591 A CN201110256591 A CN 201110256591A CN 102321717 A CN102321717 A CN 102321717A
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Abstract
The invention belongs to the technical field of corn protein deep processing, particularly discloses a preparation method for a corn bioactive peptide. According to the preparation method, corn protein is adopted as a raw material; for extending the corn deep processing industry chain and realizing high added value conversation of the corn, a useful exploration is performed. With the present invention, industry developments of high-end food and health products can be driven. The preparation method provided by the present invention has characteristics of stable reaction process, easy control, low impurity content in the final product, high amino acid content, good taste, no abnormal tastes such as bitterness and the like, and low production cost.
Description
(1) technical field
The invention belongs to the zein deep process technology, particularly a kind of preparation method of corn biologically active peptides.
(2) background technology
Corn peptide is the hydrolysate of Zein powder, is by molecular weight is very little but active very high small peptide molecular composition except that having the good advantage of solvability, also has good functional characteristics.Corn peptide compare with the Zein powder that is not hydrolyzed have the high density low viscosity characteristics, good thermostability and refrigerated stability, good whipability, hydrolysate characteristic such as bitter taste free from extraneous odour slightly, utilize these character can it be applied as industries such as common nutritive food such as protein-high fluid foodstuffs.The physiologically active that corn peptide has is as being prone to the property digested and assimilated, promoting microbial fermentation, anti-oxidant activity to can be used as additive, motion food, the elderly's food raw material and the SOD substitute of leavened foods such as producing sour milk, soy sauce, fermentation ham; Can also utilize its antifatigue, promote the alcohol metabolism isoreactivity, be developed to functional beverage such as sports beverages, sobering-up beverage.
Modern medicine circle and organic sphere are all generally acknowledged, and as meals and clinical food, the form of peptide is superior to corresponding amino acid aspect following two: (1) peptide specific absorption in digestive tube is higher than corresponding amino acid; (2) osmotic pressure of peptide is lower than corresponding amino acid.Biologically active polypeptides is exactly the peptide class that animal is had physiological function or physiological action, and therefore, the production of biologically active polypeptides and qualitative question are just becoming the research focus, develops these biomolecules and has a extensive future as new healing potion and functional food.
(3) summary of the invention
The present invention is in order to remedy the deficiency of prior art, and the preparation method of a kind of reacting balance, corn biologically active peptides that production cost is low is provided.
The present invention realizes through following technical scheme:
A kind of preparation method of corn biologically active peptides is a raw material with the Zein powder, mainly comprises the steps:
(1) Zein powder is crushed to 80 ~ 100 purpose fine powders, adds the water furnishing pulpous state of 8 ~ 15 times of weight, the autoclaving postcooling;
(2) add neutral protease, yellow soda ash and calcium chloride in the phase refrigerative slurries, autoclaving behind 45 ± 1 ℃ of following enzymolysis 1.5 ~ 3.0h, cooling;
(3) in slurries by 10% aseptic access aspergillus oryzae bacterial classification of total amount, 35 ± 1 ℃ of bottom fermentations 10 ~ 12 hours;
(4) adding weight in the material after fermentation is the water of 0.5 ~ 0.8 times of material, and adds calcium chloride and filtered, in gained filtrating, adds yellow soda ash, regulates the pH value between 6.7 ~ 7.0, and the decolouring of particle charcoal post is handled;
(5) the gained slurries filter through nf membrane again; Carry out molecular retention, molecular retention gained material is sent into storage tank, and adding weight is the water of 0.8 ~ 1.0 times of material in storage tank; Add yellow soda ash simultaneously; Keeping pH value between 6.7 ~ 7.0, and by 0.3 ~ 0.5% weightmeasurement ratio adding calcium chloride, is vacuum concentration material under 0.080 ~ 0.085MPa at 60 ~ 65 ℃, vacuum tightness;
(6) the partially concd material is added yellow soda ash and regulate the pH value between 6.7 ~ 7.0, utilize proposition equipment to carry out high purity and purify;
(7) all the other concentrated materials are sent into crystallizer, in 0.075 ~ 0.080MPa vacuum tightness, 65 ~ 70 ℃ of following crystallizations constantly replenish high purity purification liquid in the crystallisation process;
(8) crystalline product is carried out cryodrying and obtain finished product, regulating temperature of inlet air in the drying process is 90 ~ 100 ℃, and temperature out is 50 ~ 60 ℃, and temperature of charge is 66 ~ 70 ℃.
The production of corn biologically active peptides has enzyme process, microbial method, chemical synthesis.The enzyme process product has bigger bitter taste; Chemical synthesis is not suitable for suitability for industrialized production; The extracellular enzyme that microbial method not only can utilize microorganisms is decomposing protein effectively, can also remove the bitter taste at oligopeptides two ends through the decomposition anabolism of self, and therefore, the working method that the present invention adopts is a microbial method.
More excellent scheme of the present invention is:
In step (1) and (2), the autoclaving temperature is 121 ℃, and sterilization time is 10 ~ 20min, and cooling back temperature of charge is 45 ± 1 ℃.
In the step (2), the neutral protease in the adding slurries is 0.1 ~ .5 a times of Zein powder weight, and the mass concentration of yellow soda ash is 40h/L, and addition is the 16ml/g Zein powder, and the addition of calcium chloride accounts for the weightmeasurement ratio of slurry 0.8 ~ 1.1%.
In the step (4), the addition of calcium chloride accounts for the weightmeasurement ratio of fermentation materials 0.3 ~ 0.5%, and the addition of flocculating aids is 3 ~ 5kg/t Zein powder.
The present invention is raw material with the zein, has carried out useful exploration for the high added value that prolongs corn deep processing industrial chain and realization corn transforms.Can drive high-end food, health products trade development.
Preparation technology's reaction process of the present invention is steady, and than being easier to control, the foreign matter content in its finished product is low, and aminoacids content is high, mouthfeel does not have peculiar smell such as bitterness well, and its production cost is lower.
(4) description of drawings
Below in conjunction with accompanying drawing the present invention is further described.
Fig. 1 is a process flow diagram of the present invention.
(5) embodiment
Embodiment:
(1) raw material
Zein powder (CGM is commonly called as Semen Maydis grit) is to extract main by product behind the starch during W-Gum is produced; Approximately contain 60% protein, its coarse mouthfeel, poorly water-soluble; In order to improve the added value of this type raw material, improve its proteinic functional performance, reduce environmental pollution; Through deep processing production, produce the corn biologically active peptides, for starting the service of good economy and society benefit.
(2) pulverize
Zein powder is crushed to size with the fine powder through 80 ~ 100 mesh sieves with hammer crusher, adds the water furnishing pulpous state of 8 ~ 15 times of weight, 121 ℃ of autoclaving 10 ~ 20min are cooled to 45 ± 1 ℃.
(3) enzymolysis
The neutral protease that in slurries, adds 0.1 ~ 0.5 times of weight zein; Adding mass concentration is the yellow soda ash of 40g/L, and addition is about 16mL/g (Zein powder), and adds calcium chloride in 0.8 ~ 1.1% (w/v) ratio; Temperature is controlled at 45 ± 1 ℃, and enzymolysis through 121 ℃ of autoclaving 10min, is cooled to 45 ± 1 ℃ after 1.5 ~ 3.0 hours again.
(4) fermentation
By 10% aseptic access aspergillus oryzae bacterial classification of total amount, temperature is controlled at 35 ± 1 ℃, keeps to ferment in 10 ~ 12 hours in slurries.
(5) micro-filtration, decolouring
In the gained material of fermentation back, add material and weigh 0.5 ~ 0.8 times fresh water, and add calcium chloride, add the flocculating aids that weight is 3 ~ 5 kg/ton (Zein powder) simultaneously in 0.3 ~ 0.5% (w/v) ratio.The gained slurries filter through Microfilter (10 ~ 20 μ m), and in gained filtrating, adding mass concentration is the yellow soda ash of 40g/L, regulate pH value between 6.7 ~ 7.0, and the decolouring of particle charcoal post is handled.
(6) filter, concentrate
The gained slurries filter through nf membrane again; Carry out molecular retention; Molecular retention gained material gets into storage tank, and weighs 0.8 ~ 1.0 times fresh water to storage tank adding material, adds the yellow soda ash that mass concentration is 40g/L simultaneously; Keep pH value between 6.7 ~ 7.0, and add calcium chloride in 0.3 ~ 0.5% (w/v) ratio; Gained solution carries out vacuum concentration to material concentration again about 45%, in the vacuum Concentrating Process, and control material temperature 60 ~ 65 ℃, vacuum tightness 0.080 ~ 0.085Mpa.
(7) high purity is purified
Functional quality concentration is the yellow soda ash of 40g/L, and the pH value of step 6 gained filtrating is regulated and control between 6.7 ~ 7.0, utilizes equipment for purifying to carry out high purity and purifies.
(8) crystallization
Step 6 gained is concentrated material utilizes crystallizer to carry out crystallization; Crystallisation process major control processing condition: vacuum tightness 0.075 ~ 0.080MPa in jar; 65 ~ 70 ℃ of feed temperatures; Crystallisation process constantly replenishes high purity purification liquid, promotes crystalline growth to replenish solute, notes inspection crystalline growth situation constantly.
(9) cryodrying
Through cryodrying product moisture is reached between 8 ~ 10% step 8 gained crystalline product.The cryodrying caution of operation is regulated the feed amount, keeps the homodisperse blanking, regulates 90 ~ 100 ℃ of temperature of inlet air in the drying process; 50 ~ 60 ℃ of temperature outs, 66 ~ 70 ℃ of temperature of charge, and want careful observation; Find that also crystalline substance is many, the crystallization tide, problems such as Bai Liang difference in time adjust.
(10) packing
Be finished product behind the dry packing.
Claims (4)
1. the preparation method of a corn biologically active peptides is a raw material with the Zein powder, it is characterized by, and mainly comprises the steps:
(1) Zein powder is crushed to 80 ~ 100 purpose fine powders, adds the water furnishing pulpous state of 8 ~ 15 times of weight, the autoclaving postcooling;
(2) add neutral protease, yellow soda ash and calcium chloride in the phase refrigerative slurries, autoclaving behind 45 ± 1 ℃ of following enzymolysis 1.5 ~ 3.0h, cooling;
(3) in slurries by 10% aseptic access aspergillus oryzae bacterial classification of total amount, 35 ± 1 ℃ of bottom fermentations 10 ~ 12 hours;
(4) adding weight in the material after fermentation is the water of 0.5 ~ 0.8 times of material, and adds calcium chloride and filtered, in gained filtrating, adds yellow soda ash, regulates the pH value between 6.7 ~ 7.0, and the decolouring of particle charcoal post is handled;
(5) the gained slurries filter through nf membrane again; Carry out molecular retention, molecular retention gained material is sent into storage tank, and adding weight is the water of 0.8 ~ 1.0 times of material in storage tank; Add yellow soda ash simultaneously; Keeping pH value between 6.7 ~ 7.0, and by 0.3 ~ 0.5% weightmeasurement ratio adding calcium chloride, is vacuum concentration material under 0.080 ~ 0.085MPa at 60 ~ 65 ℃, vacuum tightness;
(6) the partially concd material is added yellow soda ash and regulate the pH value between 6.7 ~ 7.0, utilize proposition equipment to carry out high purity and purify;
(7) all the other concentrated materials are sent into crystallizer, in 0.075 ~ 0.080MPa vacuum tightness, 65 ~ 70 ℃ of following crystallizations constantly replenish high purity purification liquid in the crystallisation process;
(8) crystalline product is carried out cryodrying and obtain finished product, regulating temperature of inlet air in the drying process is 90 ~ 100 ℃, and temperature out is 50 ~ 60 ℃, and temperature of charge is 66 ~ 70 ℃.
2. the preparation method of corn biologically active peptides according to claim 1 is characterized in that: in step (1) and (2), the autoclaving temperature is 121 ℃, and sterilization time is 10 ~ 20min, and cooling back temperature of charge is 45 ± 1 ℃.
3. the preparation method of corn biologically active peptides according to claim 1 and 2; It is characterized in that: in the step (2); Neutral protease in the adding slurries is 0.1 ~ .5 a times of Zein powder weight; The mass concentration of yellow soda ash is 40h/L, and addition is the 16ml/g Zein powder, and the addition of calcium chloride accounts for the weightmeasurement ratio of slurry 0.8 ~ 1.1%.
4. the preparation method of corn biologically active peptides according to claim 1 and 2 is characterized in that: in the step (4), the addition of calcium chloride accounts for the weightmeasurement ratio of fermentation materials 0.3 ~ 0.5%, and the addition of flocculating aids is 3 ~ 5kg/t Zein powder.
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| CN201110256591A CN102321717A (en) | 2011-09-01 | 2011-09-01 | Preparation method for corn bioactive peptide |
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| CN201110256591A CN102321717A (en) | 2011-09-01 | 2011-09-01 | Preparation method for corn bioactive peptide |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103503992A (en) * | 2013-10-28 | 2014-01-15 | 临沂格瑞食品有限公司 | Flavored fermented milk added with corn oligopeptides and preparation method thereof |
| CN104186918A (en) * | 2014-07-29 | 2014-12-10 | 哈尔滨伟平科技开发有限公司 | Preparing method of corn gluten polypeptide for fodder |
| CN105767453A (en) * | 2016-03-21 | 2016-07-20 | 华南理工大学 | Preparation method and application of corn peptide |
| CN107365819A (en) * | 2017-08-18 | 2017-11-21 | 华南理工大学 | A kind of corn sobering-up peptide and its production and use |
| CN107997119A (en) * | 2018-02-02 | 2018-05-08 | 厦门元之道生物科技有限公司 | A kind of enzyme liquid with antialcoholism function and preparation method thereof |
| CN109574715A (en) * | 2018-12-03 | 2019-04-05 | 上海农乐生物制品股份有限公司 | A kind of method of solubility rice residue protein peptide plant nitrogen fertilizer preparation |
| CN109796518A (en) * | 2019-03-29 | 2019-05-24 | 齐齐哈尔大学 | A kind of antioxidation active peptides and preparation method thereof of microorganism and proteinase synergy effect production |
| CN112964050A (en) * | 2021-02-23 | 2021-06-15 | 协赛(山东)生物科技有限公司 | Method and equipment for quickly drying single-cell protein powder at low temperature |
| CN113061633A (en) * | 2021-03-08 | 2021-07-02 | 汕尾市五丰海洋生物科技有限公司 | Novel active peptide and preparation process thereof |
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| CN101353685A (en) * | 2008-09-05 | 2009-01-28 | 长春美能生物工程有限责任公司 | Zein active peptide and preparation technique thereof |
| CN101487038A (en) * | 2008-12-05 | 2009-07-22 | 江苏大学 | Preparation and use of maize yellow powder protein hydrolysate with hypotensive function |
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| CN101353685A (en) * | 2008-09-05 | 2009-01-28 | 长春美能生物工程有限责任公司 | Zein active peptide and preparation technique thereof |
| CN101487038A (en) * | 2008-12-05 | 2009-07-22 | 江苏大学 | Preparation and use of maize yellow powder protein hydrolysate with hypotensive function |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103503992A (en) * | 2013-10-28 | 2014-01-15 | 临沂格瑞食品有限公司 | Flavored fermented milk added with corn oligopeptides and preparation method thereof |
| CN104186918A (en) * | 2014-07-29 | 2014-12-10 | 哈尔滨伟平科技开发有限公司 | Preparing method of corn gluten polypeptide for fodder |
| CN105767453A (en) * | 2016-03-21 | 2016-07-20 | 华南理工大学 | Preparation method and application of corn peptide |
| CN105767453B (en) * | 2016-03-21 | 2020-02-18 | 华南理工大学 | Preparation method and application of corn peptide |
| CN107365819A (en) * | 2017-08-18 | 2017-11-21 | 华南理工大学 | A kind of corn sobering-up peptide and its production and use |
| CN107365819B (en) * | 2017-08-18 | 2020-09-22 | 华南理工大学 | Corn sobering-up peptide and preparation method and application thereof |
| CN107997119A (en) * | 2018-02-02 | 2018-05-08 | 厦门元之道生物科技有限公司 | A kind of enzyme liquid with antialcoholism function and preparation method thereof |
| CN109574715A (en) * | 2018-12-03 | 2019-04-05 | 上海农乐生物制品股份有限公司 | A kind of method of solubility rice residue protein peptide plant nitrogen fertilizer preparation |
| CN109796518A (en) * | 2019-03-29 | 2019-05-24 | 齐齐哈尔大学 | A kind of antioxidation active peptides and preparation method thereof of microorganism and proteinase synergy effect production |
| CN109796518B (en) * | 2019-03-29 | 2020-11-17 | 齐齐哈尔大学 | Antioxidant active peptide produced by synergistic action of microorganisms and protease and preparation method thereof |
| CN112964050A (en) * | 2021-02-23 | 2021-06-15 | 协赛(山东)生物科技有限公司 | Method and equipment for quickly drying single-cell protein powder at low temperature |
| CN113061633A (en) * | 2021-03-08 | 2021-07-02 | 汕尾市五丰海洋生物科技有限公司 | Novel active peptide and preparation process thereof |
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Application publication date: 20120118 |