CN103642885A - Method for producing wheat biological active peptide by fermentation - Google Patents
Method for producing wheat biological active peptide by fermentation Download PDFInfo
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- CN103642885A CN103642885A CN201310624196.7A CN201310624196A CN103642885A CN 103642885 A CN103642885 A CN 103642885A CN 201310624196 A CN201310624196 A CN 201310624196A CN 103642885 A CN103642885 A CN 103642885A
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- active peptides
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Abstract
The invention provides a method for producing wheat biological active peptide by fermentation. The method takes wheat vital gluten and wheat germs as raw materials; a plant macromolecular protein is converted into the biological active peptide by a microbial fermentation effect. The method comprises the following steps: pre-treating the wheat vital gluten and the wheat germs and mixing according to a ratio; sterilizing and then inoculating; fermenting; and purifying and drying. According to the method provided by the invention, the technology is developed, various technological parameters are reliable and stable, fermentation conditions are moderate, the quality of the final product is stable and the wheat biological active peptide has obvious biological functional characteristics; effective active components of leftovers in industrial processing of wheat are sufficiently protected and utilized to realize the effective appreciation of byproducts generated by deep processing of wheat, and the social benefits and the economic benefits are very obvious.
Description
Technical field
The present invention relates to wheat protein deep process technology field, be specifically related to a kind of method of fermentative Production wheat biologically active peptides.
Background technology
The main component of gluten powder is wheat-gluten, it is comparatively complicated protein hydrate, in gluten except containing a small amount of fat, carbohydrate, starch, lipoid cpd, mainly contain molecular weight, be spherical, have better ductile prolamine and molecular weight large, become fibrous, have compared with the glutenin of strong elasticity and form.It is nutritious, inexpensive vegetable protein source.Wheatgerm accounts for 3% left and right of whole wheat grain.Wheat plantule protein matter content is high, and more than 25%, white protein matter reaches total protein 26%, and globular protein reaches 20%, and protein,alcohol-soluble matter reaches 14%, and gluten matter reaches 0.4%, and other is water insoluble protein 39 .6%., therefore, wheatgerm contains abundant nutritive ingredient to supply with Growth of Wheat.In addition in wheatgerm, contain lipid, carbohydrate, VITAMIN and mineral substance, be suitable for human body utilization and HUMAN HEALTH is had to good guaranteeing role.
Carry out in the enterprise of deep processing as raw material take wheat, as produced starch, the factory of the products such as β-amylose, organic acid, amino acid, all can produce a large amount of tankage, in these tankage, approximately can isolate the albumen that accounts for raw material weight 5%～6%.According to incompletely statistics, only the annual production of China's gluten powder is more than 600,000 tons, yet, there is considerable factory not do any utilization and discharge naturally, the annual albumen with discharging of waste liquid, up to more than 100,000 tons, had both been wasted valuable grain resource, had caused again environmental pollution.
Protein can produce the peptide with unique physiologically active after enzymolysis, i.e. biologically active peptides, and its use value is very high, also has physiological function widely, as the absorption of can be hypotensive, reducing cholesterol, Promote immunity and promoting nutritive substance.
At present, research from scholar, the domestic research to biologically active peptides focuses mostly on and in the zymin with single, carries out the optimization of enzymolysis processing or enzymolysis process, but due to the randomness of enzymolysis process and the volatility of enzymolysis product, diversity, complicacy, at present, protein digestion have that speed is slow, hydrolysis not thoroughly, hydrolyzed solution bitter, target component content is low and product is difficult to the problems such as separated.The present invention adopts fermentative Production wheat protein biologically active peptides, by microbial process, some bitter peptides group is modified and is recombinated, and makes between little peptide, moves between little peptide and amino acid and connect, reset.It is good that the wheat protein biologically active peptides making has solvability, and without bitter taste and peculiar smell, mouthfeel is good, dissolves viscosity little, and be heated and the advantage such as do not solidify, and mild condition, technique is advanced, and cost is lower.
Summary of the invention
For problems of the prior art, the invention provides a kind of method of fermentative Production wheat biologically active peptides.
For achieving the above object, the technical solution used in the present invention is:
A method for fermentative Production wheat biologically active peptides, comprises the following steps: (1) is with wheat gluten flour (enzymolysis pre-treatment), wheatgerm (wheatgerm is crushed to 60 order-120 orders), with wherein one or both arbitrary proportions mixing; (2) adding water, to be mixed with weight percent concentration be 5%-20%, and adjusting pH value is 5.5-7.5; (3) then at 106 ℃-121 ℃, carry out sterilizing 15 minutes-25 minutes, be cooled to 30 ℃-40 ℃, by volume per-cent is that ripe subtilis liquid spawn is cultivated in 0.8%-12.0% access; (4) then put into fermentation cylinder for fermentation, pass into sterile air in fermentor tank, stir fermentation, stir revolution 80-200rpm, maintain the temperature at 30 ℃-40 ℃ during fermentation, fermentation period is 18-100 hour; (5) after fermentation ends, rapidly temperature is risen to 42 ℃-65 ℃, keep 1.5-5.0 hour, and then temperature is risen to 106 ℃-121 ℃ carry out high-temperature inactivation, after high-temperature inactivation, first carry out centrifugation slagging-off, it is 20%-45% that supernatant liquor is concentrated into volume ratio through PES membrane filtration again, and dialysis carries out that spray dried is dry makes Powdered wheat biological activity peptide product after desalination.
In described step (1), using gluten powder during as Primary Fermentation raw material, it is carried out to pre-treatment: the gluten powder slurry of preparation 60%-80%, then after adding 0.6% proteolytic enzyme (with respect to the quality of gluten powder) to stir, add 1.2 ‰ amylase, stir evenly.In 60 ℃ of insulation 1-3 hour, lower the temperature 40 ℃ and add 1.5 ‰ saccharifying enzyme (with respect to the quality of gluten powder), normal temperature preservation is stand-by.
Subtilis in described step (3) is that subtilis (Bacillus subtilis) is the bacterial classification of experiment screening institute of our unit preservation.
Slant culture method in described step (3) is: be weight percentage below, and extractum carnis 0.5%-12.0%, peptone 0.5%-12.0%, NaCl0.3%-5.0%, agar 1.0%-3.0%, water is solvent, pH5.5-7.5; 121 ℃ of sterilizings 20 minutes, are put into inclined-plane cooling, inoculate rear 29 ℃ of-38 ℃ of standing cultivation 12-48 hour, standby; Triangular flask liquid cultivating method is: be weight percentage below, and the gluten powder of processing slurry 10.0%-25.0%, wheatgerm 1.0%-6.0%, KH2PO40.1%-5.0%, MgSO40.05%-2.0%, NaCl0.3%-5.0%, stir, pH5.5-7.5; 106 ℃ of-121 ℃ of sterilizing 15-25 minute, are cooled to 30 ℃-45 ℃, and access is through the Bacillus subtilis strain of slant culture, and 29 ℃ of-38 ℃ of shaking tables are cultivated 12-72 hour, for fermentor tank, inoculate.
After centrifugation slagging-off in described step (5), first cross the PES film of MW10000, after the PES of MW300 film, after dialysis desalination, the dry wheat biologically active peptides of making of spraying.
Compared with prior art, the present invention can reduce by product, and technique is simple, cost is low, good, the water-soluble height of wheat biologically active peptides mouthfeel that makes, is more conducive to production and the processing of industrialization; And the present invention adopts fermentative Production, fermented supernatant fluid can be condensed into protein fodder, outside a small amount of cleaning of evaporator water, without processing wastewater discharge, also there is no other pollutent, production technique environmental protection, thus turn waste into wealth, increase value-added content of product, reduce production costs.
Embodiment
Embodiment mono-
The preparation of slant medium and spawn culture:
Percentage ratio by weight, extractum carnis 3.0%, peptone 1.5%, NaCl0.3%, agar 1.5%, water is solvent, pH7.0.121 ℃ of sterilizings 20 minutes, are put into inclined-plane cooling, inoculate rear 37 ℃ of standing cultivations 26 hours, standby.
The preparation of triangular flask liquid nutrient medium and spawn culture:
Be below weight percentage, the gluten powder slurry 10.0% of processing, wheatgerm 3.0%, KH2PO42.0%, MgSO40.8%, NaCl0.3%, stirs, pH7.0; 106 ℃ of-121 ℃ of sterilizings 20 minutes, are cooled to 37 ℃, and access is through the Bacillus subtilis strain of slant culture, and 37 ℃ of shaking tables are cultivated 28 hours, for fermentor tank, inoculate.
The preparation of fermentation tank culture medium and fermentation are controlled:
Preparation 70% gluten powder slurry, then after adding 0.6% proteolytic enzyme (with respect to the quality of gluten powder) to stir, add 1.2 ‰ amylase, stir evenly.In 60 ℃ of insulations 2 hours, lower the temperature 40 ℃ and add 1.5 ‰ saccharifying enzyme (with respect to the quality of gluten powder), adding water, to be mixed with weight percent concentration be that 7%(is with respect to the quality of gluten powder), adjusting pH value is 7.0, then 121 ℃ of sterilizings are 20 minutes, be cooled to 37 ℃, by volume per-cent is that ripe subtilis liquid spawn is cultivated in 10.0% access.In fermentor tank, pass into sterile air, stir fermentation, ferment first 5 hours, stir revolution 100rpm, fermentation, to 6 hours-24 hours, is stirred revolution 160rpm, and fermentation, after 24 hours, is stirred revolution 140rpm, constantly adjusts the air flow of sterile air during this.During fermentation, temperature is controlled at 37 ℃, and fermentation period is 72 hours.
After fermentation ends, temperature is risen to 42 ℃ rapidly, keep 3.0 hours.And then temperature is risen to 115 ℃ carry out high-temperature inactivation, after high-temperature inactivation, after centrifugation slagging-off, first cross the PES film of MW10000, after the PES of MW300 film, after dialysis desalination, the dry wheat biologically active peptides of making of spraying.The extraction rate reached of protein is to 95.46wt%, and yield is 83%.
Embodiment bis-
The preparation of the preparation of slant medium and spawn culture, triangular flask liquid nutrient medium and spawn culture are with example one.
The preparation of fermentation tank culture medium and fermentation are controlled:
The gluten powder slurry of preparation 60%, after adding again 0.7% proteolytic enzyme (with respect to the quality of gluten powder) to stir, add 1.0 ‰ amylase, stir evenly, in 60 ℃ of insulations 3 hours, lower the temperature 40 ℃ and add 1.5 ‰ saccharifying enzyme (with respect to the quality of gluten powder), wheatgerm is crushed to 100 orders, adding water, to be mixed with weight percent concentration be that 6%(is with respect to gluten powder and wheatgerm total amount, gluten powder: wheatgerm is 4:1), adjusting pH value is 7.0, then 121 ℃ of sterilizings are 20 minutes, be cooled to 37 ℃, by volume per-cent is that ripe subtilis liquid spawn is cultivated in 12.0% access.In fermentor tank, pass into sterile air, stir fermentation, ferment first 5 hours, stir revolution 100rpm, fermentation, to 6 hours-24 hours, is stirred revolution 160rpm, and fermentation, after 24 hours, is stirred revolution 140rpm, constantly adjusts the air flow of sterile air during this.During fermentation, temperature is controlled at 37 ℃, and fermentation period is 96 hours.
After fermentation ends, temperature is risen to 42 ℃ rapidly, keep 3.0 hours.And then temperature is risen to 115 ℃ carry out high-temperature inactivation, after high-temperature inactivation, after centrifugation slagging-off, first cross the PES film of MW10000, after the PES of MW300 film, after dialysis desalination, the dry wheat biologically active peptides of making of spraying.The extraction rate reached of protein is to 93.26wt%, and yield is 76%.
Embodiment tri-
The preparation of the preparation of slant medium and spawn culture, triangular flask liquid nutrient medium and spawn culture are with example one.
The preparation of fermentation tank culture medium and fermentation are controlled:
Wheatgerm is crushed to 100 orders, adding water, to be mixed with weight percent concentration be that 10%(is with respect to the quality of wheatgerm), adjusting pH value is 7.0, then 121 ℃ of sterilizings are 20 minutes, be cooled to 37 ℃, by volume per-cent is that ripe subtilis liquid spawn is cultivated in 8.0% access.In fermentor tank, pass into sterile air, stir fermentation, ferment first 5 hours, stir revolution 100rpm, fermentation, to 6 hours-24 hours, is stirred revolution 160rpm, and fermentation, after 24 hours, is stirred revolution 140rpm, constantly adjusts the air flow of sterile air during this.During fermentation, temperature is controlled at 37 ℃, and fermentation period is 48 hours.
After fermentation ends, temperature is risen to 42 ℃ rapidly, keep 3.0 hours.And then temperature is risen to 115 ℃ carry out high-temperature inactivation, after high-temperature inactivation, after centrifugation slagging-off, first cross the PES film of MW10000, after the PES of MW300 film, after dialysis desalination, the dry wheat biologically active peptides of making of spraying.The extraction rate reached of protein is to 89.74wt%, and yield is 20%.
Claims (5)
1. a method for fermentative Production wheat biologically active peptides, is characterized in that comprising the following steps (1)
With wheat gluten flour (enzymolysis pre-treatment), wheatgerm (wheatgerm is crushed to 60 order-120 orders), with wherein one or both arbitrary proportions mixing; (2) adding water, to be mixed with weight percent concentration be 5%-20%, and adjusting pH value is 5.5-7.5; (3) then at 106 ℃-121 ℃, carry out sterilizing 15 minutes-25 minutes, be cooled to 30 ℃-40 ℃, by volume per-cent is that ripe subtilis liquid spawn is cultivated in 0.8%-12.0% access; (4) then put into fermentation cylinder for fermentation, pass into sterile air in fermentor tank, stir fermentation, stir revolution 80-200rpm, maintain the temperature at 30 ℃-40 ℃ during fermentation, fermentation period is 18-100 hour; (5) after fermentation ends, rapidly temperature is risen to 42 ℃-65 ℃, keep 1.5-5.0 hour, and then temperature is risen to 106 ℃-121 ℃ carry out high-temperature inactivation, after high-temperature inactivation, first carry out centrifugation slagging-off, it is 20%-45% that supernatant liquor is concentrated into volume ratio through PES membrane filtration again, and dialysis carries out that spray dried is dry makes Powdered wheat biological activity peptide product after desalination.
2. according to the method for fermentative Production wheat biologically active peptides according to claim 1, it is characterized in that: using gluten powder during as Primary Fermentation raw material, it is carried out to pre-treatment: the gluten powder slurry of preparation 60%-80%, after adding again 0.6% proteolytic enzyme (with respect to the quality of gluten powder) to stir, add 1.2 ‰ amylase, stir evenly.In 60 ℃ of insulation 1-3 hour, lower the temperature 40 ℃ and add 1.5 ‰ saccharifying enzyme (with respect to the quality of gluten powder), normal temperature preservation is stand-by.
3. the method for fermentative Production wheat biologically active peptides according to claim 1, is characterized in that: described subtilis is that subtilis (Bacillus subtilis) is the bacterial classification of experiment screening institute of our unit preservation.
4. according to the method for the fermentative Production wheat biologically active peptides described in claim 1,2, it is characterized in that: described slant culture method is: be weight percentage below, extractum carnis 0.5%-12.0%, peptone 0.5%-12.0%, NaCl 0.3%-5.0%, agar 1.0%-3.0%, water is solvent, pH5.5-7.5; 121 ℃ of sterilizings 20 minutes, are put into inclined-plane cooling, inoculate rear 29 ℃ of-38 ℃ of standing cultivation 12-48 hour, standby; Triangular flask liquid cultivating method is: be weight percentage below, and the gluten powder of processing slurry 10.0%-25.0%, wheatgerm 1.0%-6.0%, KH
2pO
40.1%-5.0%, MgSO
40.05%-2.0%, NaCl 0.3%-5.0%, stirs, and pH5.5-7.5; 106 ℃ of-121 ℃ of sterilizing 15-25 minute, are cooled to 30 ℃-45 ℃, and access is through the Bacillus subtilis strain of slant culture, and 29 ℃ of-38 ℃ of shaking tables are cultivated 12-72 hour, for fermentor tank, inoculate.
5. the method for fermentative Production wheat biologically active peptides according to claim 1, it is characterized in that: after described centrifugation slagging-off, first cross the PES film of MW10000, after the PES of MW300 film, after dialysis desalination, the dry wheat biologically active peptides of making of spraying.
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| CN201310624196.7A CN103642885A (en) | 2013-11-26 | 2013-11-26 | Method for producing wheat biological active peptide by fermentation |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103948137A (en) * | 2014-05-12 | 2014-07-30 | 新疆农业大学 | Complex dietary fiber solid beverage and preparation method thereof |
| CN104523532A (en) * | 2015-01-16 | 2015-04-22 | 北京七秀时代科技有限公司 | Method for extracting micromolecule active component of wheat germ, extract and application of extract |
| CN104745650A (en) * | 2015-04-13 | 2015-07-01 | 郑州新威营养技术有限公司 | Preparation method of combined-state glutamine used for improving nutrients of piglet intestines |
| CN104996715A (en) * | 2015-07-08 | 2015-10-28 | 青岛嘉瑞生物技术有限公司 | A processing method for preparing wheat germ polypeptide by a compound fermentation method |
| CN105543317A (en) * | 2016-02-17 | 2016-05-04 | 成都美溢德生物技术有限公司 | Method for liquid synchronous fermentation and enzymolysis of plant protein and prepared protein hydrolysate |
-
2013
- 2013-11-26 CN CN201310624196.7A patent/CN103642885A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103948137A (en) * | 2014-05-12 | 2014-07-30 | 新疆农业大学 | Complex dietary fiber solid beverage and preparation method thereof |
| CN104523532A (en) * | 2015-01-16 | 2015-04-22 | 北京七秀时代科技有限公司 | Method for extracting micromolecule active component of wheat germ, extract and application of extract |
| CN104523532B (en) * | 2015-01-16 | 2017-05-10 | 北京七巧时代科技有限公司 | Method for extracting micromolecule active component of wheat germ, extract and application of extract |
| CN104745650A (en) * | 2015-04-13 | 2015-07-01 | 郑州新威营养技术有限公司 | Preparation method of combined-state glutamine used for improving nutrients of piglet intestines |
| CN104745650B (en) * | 2015-04-13 | 2018-02-02 | 郑州新威营养技术有限公司 | For the preparation method for the reference state glutamine for improving nutrients in intestine of young pigs |
| CN104996715A (en) * | 2015-07-08 | 2015-10-28 | 青岛嘉瑞生物技术有限公司 | A processing method for preparing wheat germ polypeptide by a compound fermentation method |
| CN105543317A (en) * | 2016-02-17 | 2016-05-04 | 成都美溢德生物技术有限公司 | Method for liquid synchronous fermentation and enzymolysis of plant protein and prepared protein hydrolysate |
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Application publication date: 20140319 |