CN105624249A - Preparation method of aquatic protein and plant protein compound active peptide - Google Patents
Preparation method of aquatic protein and plant protein compound active peptide Download PDFInfo
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- CN105624249A CN105624249A CN201610129680.6A CN201610129680A CN105624249A CN 105624249 A CN105624249 A CN 105624249A CN 201610129680 A CN201610129680 A CN 201610129680A CN 105624249 A CN105624249 A CN 105624249A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
Abstract
The invention discloses a preparation method of aquatic protein and plant protein compound active peptide. The method comprises the following steps that the fresh aquatic protein is ground for use, and the plant protein is smashed and sieved through a 60-mesh sieve for use; the ground aquatic protein and the mashed plant feed protein are mixed, and nutrients and water are added and mixed to be uniform to serve as a fermentation medium; Bacillus subtilis HL-2 which can produce high-temperature-resistant protease is inoculated to the fermentation medium; the medium inoculated with the strain is subjected to solid aerobic fermentation for 38 h-48 h at the temperature of 50 DEG C-55 DEG C; water is added to a solid fermentation product, enzymolysis is conducted for 2 h at 55 DEG C; a liquid enzymolysis product is subjected to filtration, ultrafiltration and nanofiltration in sequence to obtain a concentrated solution, freezing and drying are conducted on the concentrated solution, and an active peptide product is obtained. According to the preparation method of the aquatic protein and plant protein compound active peptide, the action condition is mild, the biological activity of other functional ingredients contained in the prepared functional peptide and the raw materials themselves is more preserved, the preparation process is simplified, the production cost of the active peptide is reduced, and the good application prospect is achieved.
Description
Technical field
The present invention relates to the preparation method of a kind of compound bioactive peptide of aquatic product protein-vegetable-protein, belong to processing of aquatic products and utilize field.
Background technology
The little peptide of product after protein digestion not only readily digested, absorb, but also there is hypertension, anti-cholesterol, antithrombus formation, improve lipid metabolism, strengthen human body physical agility, help to recover tired, promote the absorption of calcium phosphorus and other trace element, promote brain development, improve memory, strengthen scavenger cell and B cell vigor, strengthen immunologic function, protect epidermic cell, prevent melanin deposition, eliminate the effects such as interior free yl.
About the preparation of aquatic product protein bioactive peptide, domestic carry out big quantifier elimination. At present, the preparation method of aquatic product protein bioactive peptide mainly contains the method these four kinds that self-dissolving enzymolysis, enzymolysis process, liquid fermentation method and solid state fermentation combine with liquid enzymolysis. A kind of method that patent application 200810195646.4 technique of bioactive micro peptide " endogenous enzyme prepare " discloses endogenous enzyme self-dissolving enzymolysis utilized in marine low-value fish body prepares the method for aquatic product protein bioactive micro peptide. Patent application 201410145196.3 " a kind of method preparing scallop body bioactive peptide " discloses a kind of method that enzymolysis process prepares aquatic product protein bioactive peptide. Patent application CN102028091A " a kind of method of preparing low molecular weight fish peptide through Bacillus natto fermentation method " discloses a kind of method that solution fermentation prepares fish protein peptide, and patent application 201210539941.3 " a kind of by fish protein peptide and the production method of protein peptide that forms with soybean peptides " discloses a kind of combining with liquid enzymolysis by solid state fermentation and prepares the method for aquatic product protein bioactive peptide.
There is following defect in existing aquatic product protein bioactive peptide technology of preparing. First, existing aquatic product protein bioactive peptide technology of preparing has the process of pyroprocessing. Above technology of preparing there is high temperature go out this operation of enzyme; The method raw material of fermentable needs sterilizing. These pyroprocessing above, the biologic activity of meeting in functional peptides or aquatic product protein itself other functional ingredient contained to a certain degree weakening preparation; High-temperature process in addition, can strengthen Mei Lade so that enzymolysis solution color becomes dark, for the finished product course of processing adds burden. Secondly, the method for additional enzyme enzymolysis needs added proteins enzyme, adds cost; Self-dissolving enzymolysis, due to the deficiency of endogenous enzyme, causes enzymolysis not thorough. In addition, although microbe fermentation method is without added proteins enzyme, but raw material needs sterilizing to cause endogenous enzyme inactivation, can not fully effectively utilize endogenous enzyme.
Summary of the invention
For existing aquatic product protein bioactive peptide technology of preparing Problems existing. The main purpose of the present invention is to provide a kind of method preparing aquatic product protein bioactive peptide of low temperature, to retain the functional peptides of preparation and the biologic activity of the contained functional ingredient of aquatic product protein itself better. It is a further object of the present invention to provide a kind of method that fermentation method prepares bioactive peptide so that fermentable and endogenous enzyme enzymolysis process can organically combine. The present invention also has an object to be the preparation section simplifying bioactive peptide, reduces its production cost.
In order to solve the problem, the technical solution adopted in the present invention is as follows: the preparation method of a kind of compound bioactive peptide of aquatic product protein-vegetable-protein, comprises the steps:
(1) in the LB liquid nutrient medium cooled after subtilis (Bacillussubtilis) HL-2 that can produce thermostable proteinase of slant preservation is linked into sterilizing, in 55 DEG C, 150r/min cultivate 12h, be prepared into subtilis (Bacillussubtilis) HL-2 seed liquor for subsequent use;
(2) by for subsequent use after the rubbing of fresh aquatic product protein, 60 orders sieves are crossed after being pulverized by vegetable-protein for subsequent use;
(3) ratio that the vegetable-protein of rubbing aquatic product protein obtained for step (2) and pulverizing is 0.5 ~ 1.5:1 in mass ratio is mixed, and Ensure Liquid thing and water mix even, obtain final moisture content and control the fermention medium in 40%��80% scope;
(4) on the step fermention medium that so (3) are obtained, subtilis (Bacillussubtilis) the HL-2 seed liquor prepared by step (1) is accessed, and mixed even;
(5) solid state fermentation: the fermention medium adding bacterium that step (4) obtains is fermented according to following method: fermention medium is divided in the fermentation tray sterilized, charging thickness is 4cm��8cm, the wet gauze of fermention medium surface coverage sterilizing in tray, again tray is placed in and puts frame top fermentation in the solid fermentation room of sterilizing, control envrionment temperature is at 50 DEG C ~ 55 DEG C, and fermentation time is 38h��48h;
(6) liquid enzymolysis: step (5) obtaining solid state fermentation product with water in mass ratio for the ratio of 1:3 mixes, and join in enzymatic vessel after stirring evenly, controlled enzymatic hydrolysis temperature is 55 DEG C, enzymolysis 2h;
(7) processing of finished product: the liquid enzyme hydrolysis products that step (6) obtains is carried out filters removal precipitation and obtains enzymolysis solution; The ultrafiltration removal macro-molecular protein that enzymolysis solution is the ultra-filtration membrane of 5000 dalton by molecular weight cut-off obtains permeate; The nanofiltration removal amino acid that then permeate is the nanofiltration membrane of 200 dalton by molecular weight cut-off obtains concentrated solution; Concentrated solution obtains bioactive peptide finished product through lyophilize.
Subtilis (Bacillussubtilis) HL-2 that can produce thermostable proteinase used in described step (1) is preserved in Guangdong Province's Culture Collection on January 18th, 2016, deposit number is GDMCCNo:60001, and preservation address is Guangdong Microbes Inst, compound 5th floors, No. 59 building, No. 100, Xianlie Middle Road, Guangzhou City.
Aquatic product protein in described step (2) is the one in leftovers of tilapia, krill, shrimp head, oyster, scallop body, cod tankage, squid tankage, Harengulazunasi (Bleeker) tankage, black carp tankage, silver carp tankage, grass carp tankage and stripped tuna tankage. Vegetable-protein in described step (2) is a kind of or wherein two or more mixture in dregs of beans, peanut meal, cottonseed meal and the dish dregs of rice.
Nutrition in described step (3) is the mixture of Semen Maydis powder, food-grade calcium chloride, food grade iron protochloride and food grade phosphoric acid hydrogen two potassium composition. Nutraceutical add-on in described step (3) accounts for fermention medium total mass per-cent in the nutraceutical quality added, it is respectively: Semen Maydis powder 0.01%��5%, food-grade calcium chloride 0.01%��5%, food grade iron protochloride 0.01%��5%, food grade phosphoric acid hydrogen two potassium 0.01%��5%.
In described step (4), the inoculum size of subtilis (Bacillussubtilis) HL-2 seed liquor accounts for fermention medium total mass per-cent to access the quality of seed liquor, is 1%��15%.
Compared with prior art, the present invention has the following advantages.
(1) whole preparation process does not have pyroprocessing, remains the biologic activity in the functional peptides of preparation and aquatic product protein other functional ingredient contained itself better. The present patent application adopts high temperature resistant fermentation of bacillus subtilis, can suppress the growth of pathogenic bacterium and spoilage organism under the high temperature conditions, therefore in this technology substratum without the need to sterilizing. In addition, this technology adopts ultra-filtration membrane to remove the proteolytic enzyme in enzymolysis solution, it is not necessary to high temperature goes out enzyme.
(2) simplify operation, reduce cost. Owing to the present patent application does not have high temperature to go out the process of enzyme, this not only reduces energy consumption, but also can greatly reduce the Maillard reaction of enzymolysis solution, so the color of enzymolysis solution is more shallow. And follow-up membrane sepn has certain decolorizing effect, therefore this technology without the need to this program of decolouring, can simplify operation, reduce cost.
(3) perfect adaptation of endogenous enzyme enzymolysis and fermentable. In existing fermentation technique, raw material all needs sterilizing, and this not only adds energy consumption, and makes again the endogenous enzyme inactivation of aquatic product protein. In this technology, raw material is without the need to sterilizing, remains the activity of endogenous enzyme in aquatic product protein. Further, the temperature of fermentable is consistent with the peak enzymolysis-ability temperature of endogenous enzyme so that endogenous enzyme enzymolysis is together with fermentable energy perfect adaptation. Just owing to the present invention making full use of the enzymolysis of endogenous enzyme so that the present invention is without the need to separately adding proteolytic enzyme.
(4) preparation cost not only to be combined liquid zymolysis technique lower than existing solid state fermentation, and ferment effect is also better than the liquid zymolysis technique of existing solid state fermentation associating. Compared with the technology that existing employing fermentation method prepares bioactive peptide, the present patent application raw material without the need to extinction, capable of reducing energy consumption and cost; The technical scheme provided due to the present patent application is become extinct without the need to high temperature, the enzyme remaining aquatic product protein endogenous proteinase is lived, this is that existing employing normal temperature fermentation bacterial classification is prepared bioactive peptide and cannot be accomplished, under the assistance of aquatic product protein endogenous proteinase, the present patent application can without added proteins enzyme. At high temperature the activity of proteolytic enzyme enzyme is stronger, and therefore, this technology adopts high temperature resistant bacterial classification to ferment, and the degree of hydrolysis of aquatic product protein is higher than existing normal temperature strain fermentation technology.
The present invention provides the preparation method of a kind of compound bioactive peptide of aquatic product protein-vegetable-protein. The technology of the present invention action condition is gentle, remains the biologic activity in the functional peptides of preparation and aquatic product protein other functional ingredient contained itself more, and simplifies preparation section and reduce the production cost of bioactive peptide, has good application prospect.
Embodiment
In order to understand the present invention better, with regard to the selection of the kind of the kind of aquatic product protein, vegetable-protein, fermentation parameter and fermentation mode, the invention will be further described in conjunction with the embodiments below, but protection scope of the present invention is not limited to this.
Embodiment 1: in the LB liquid nutrient medium cooled after subtilis (Bacillussubtilis) HL-2 that can produce thermostable proteinase of slant preservation is linked into sterilizing, in 55 DEG C, 150r/min cultivate 12h, be prepared into subtilis (Bacillussubtilis) HL-2 seed liquor for subsequent use. Fresh krill is rubbed rear for subsequent use; 60 order sieves are crossed for subsequent use after being pulverized by dregs of beans. 100 kilograms of krills rubbed and 100 kilograms are pulverized and crosses the dregs of beans mixing that 60 orders sieve, and add 5 kilograms of Semen Maydis powder, 400 grams of food-grade calcium chlorides, 300 grams of food grade iron protochlorides, 600 grams of food grade phosphoric acid hydrogen two potassium mix even together with 10 kg of water, obtain mixed even after fermention medium; Fermentation culture after mixed even adds 9 kilograms of subtilis (Bacillussubtilis) HL-2 seed liquor, stirs and mixes; Fermention medium after inoculation is divided in in the fermentation tray of the alcohol surface sterilization of 75%, thickness of feeding in tray is 8 centimetres, the wet gauze of fermention medium surface coverage sterilising treatment in tray, again tray is placed in and puts frame top fermentation in the solid fermentation room of sterilizing, control envrionment temperature is 50 DEG C, and fermentation time is 48h; After fermentation ends, the ratio that solid state fermentation product and water are 1:3 in mass ratio being mixed and stir evenly, join in enzymatic vessel, controlled enzymatic hydrolysis temperature is 55 DEG C, enzymolysis 2h; Liquid enzyme hydrolysis products obtained above carrying out filter removal precipitation and obtains enzymolysis solution, the ultrafiltration removal macro-molecular protein that enzymolysis solution is the ultra-filtration membrane of 5000 dalton by molecular weight cut-off obtains permeate; The nanofiltration removal amino acid that then permeate is the nanofiltration membrane of 200 dalton by molecular weight cut-off obtains concentrated solution; Concentrated solution obtains bioactive peptide finished product through lyophilize.
Embodiment 2: in the LB liquid nutrient medium cooled after subtilis (Bacillussubtilis) HL-2 that can produce thermostable proteinase of slant preservation is linked into sterilizing, in 55 DEG C, 150r/min cultivate 12h, be prepared into subtilis (Bacillussubtilis) HL-2 seed liquor for subsequent use. The shrimp head of fresh Penaeus vannamei is rubbed rear for subsequent use; 60 order sieves are crossed for subsequent use after being pulverized by peanut meal. 100 kilograms of shrimp heads rubbed and 100 kilograms are pulverized and crosses the peanut meal mixing that 60 orders sieve, and add 3 kilograms of Semen Maydis powder, 500 grams of food-grade calcium chlorides, 400 grams of food grade food grade iron protochlorides, 500 grams of food grade phosphoric acid hydrogen two potassium mix even together with 8 kg of water, obtain mixed even after fermention medium; Fermentation culture after mixed even adds 8 kilograms of subtilis (Bacillussubtilis) HL-2 seed liquor, stirs and mixes; Fermention medium after inoculation is divided in in the fermentation tray of the alcohol surface sterilization of 75%, thickness of feeding in tray is 4 centimetres, the wet gauze of fermention medium surface coverage sterilising treatment in tray, again tray is placed in and puts frame top fermentation in the solid fermentation room of sterilizing, control envrionment temperature is 55 DEG C, and fermentation time is 42h; After fermentation ends, the ratio that solid state fermentation product and water are 1:3 in mass ratio being mixed and stir evenly, join in enzymatic vessel, controlled enzymatic hydrolysis temperature is 55 DEG C, enzymolysis 2h; Liquid enzyme hydrolysis products obtained above carrying out filter removal precipitation and obtains enzymolysis solution, the ultrafiltration removal macro-molecular protein that enzymolysis solution is the ultra-filtration membrane of 5000 dalton by molecular weight cut-off obtains permeate; The nanofiltration removal amino acid that then permeate is the nanofiltration membrane of 200 dalton by molecular weight cut-off obtains concentrated solution; Concentrated solution obtains bioactive peptide finished product through lyophilize.
Embodiment 3: in the LB liquid nutrient medium cooled after subtilis (Bacillussubtilis) HL-2 that can produce thermostable proteinase of slant preservation is linked into sterilizing, in 55 DEG C, 150r/min cultivate 12h, be prepared into subtilis (Bacillussubtilis) HL-2 seed liquor for subsequent use. After oyster after fresh shelling is rubbed for subsequent use; 60 order sieves are crossed for subsequent use after being pulverized by dregs of beans. 100 kilograms of oysters rubbed and 100 kilograms are pulverized and crosses the dregs of beans mixing that 60 orders sieve, and add 2 kilograms of Semen Maydis powder, 550 grams of food-grade calcium chlorides, 450 grams of food grade food grade iron protochlorides, 550 grams of food grade phosphoric acid hydrogen two potassium mix even together with 6 kg of water, obtain mixed even after fermention medium; Fermentation culture after mixed even adds 9 kilograms of subtilis (Bacillussubtilis) HL-2 seed liquor, stirs and mixes; Fermention medium after inoculation is divided in in the fermentation tray of the alcohol surface sterilization of 75%, thickness of feeding in tray is 6 centimetres, the wet gauze of fermention medium surface coverage sterilising treatment in tray, again tray is placed in and puts frame top fermentation in the solid fermentation room of sterilizing, control envrionment temperature is 53 DEG C, and fermentation time is 45h; After fermentation ends, the ratio that solid state fermentation product and water are 1:3 in mass ratio being mixed and stir evenly, join in enzymatic vessel, controlled enzymatic hydrolysis temperature is 55 DEG C, enzymolysis 2h; Liquid enzyme hydrolysis products obtained above carrying out filter removal precipitation and obtains enzymolysis solution, the ultrafiltration removal macro-molecular protein that enzymolysis solution is the ultra-filtration membrane of 5000 dalton by molecular weight cut-off obtains permeate; The nanofiltration removal amino acid that then permeate is the nanofiltration membrane of 200 dalton by molecular weight cut-off obtains concentrated solution; Concentrated solution obtains bioactive peptide finished product through lyophilize.
Claims (7)
1. the present invention discloses the preparation method of a kind of compound bioactive peptide of aquatic product protein-vegetable-protein, it is characterised in that: the method comprises the steps:
(1) in the LB liquid nutrient medium cooled after subtilis (Bacillussubtilis) HL-2 that can produce thermostable proteinase of slant preservation is linked into sterilizing, in 55 DEG C, 150r/min cultivate 12h, be prepared into subtilis (Bacillussubtilis) HL-2 seed liquor for subsequent use;
(2) by for subsequent use after the rubbing of fresh aquatic product protein, 60 orders sieves are crossed after being pulverized by vegetable-protein for subsequent use;
(3) ratio that the vegetable-protein of rubbing aquatic product protein obtained for step (2) and pulverizing is 0.5 ~ 1.5:1 in mass ratio is mixed, and Ensure Liquid thing and water mix even, obtain final moisture content and control the fermention medium in 40%��80% scope;
(4) on the step fermention medium that so (3) are obtained, subtilis (Bacillussubtilis) the HL-2 seed liquor prepared by step (1) is accessed, and mixed even;
(5) solid state fermentation: the fermention medium adding bacterium that step (4) obtains is fermented according to following method: fermention medium is divided in the fermentation tray sterilized, charging thickness is 4cm��8cm, the wet gauze of fermention medium surface coverage sterilizing in tray, again tray is placed in and puts frame top fermentation in the solid fermentation room of sterilizing, control envrionment temperature is at 50 DEG C ~ 55 DEG C, and fermentation time is 38h��48h;
(6) liquid enzymolysis: step (5) obtaining solid state fermentation product with water in mass ratio for the ratio of 1:3 mixes, and join in enzymatic vessel after stirring evenly, controlled enzymatic hydrolysis temperature is 55 DEG C, enzymolysis 2h;
(7) processing of finished product: the liquid enzyme hydrolysis products that step (6) obtains is carried out filters removal precipitation and obtains enzymolysis solution; The ultrafiltration removal macro-molecular protein that enzymolysis solution is the ultra-filtration membrane of 5000 dalton by molecular weight cut-off obtains permeate; The nanofiltration removal amino acid that then permeate is the nanofiltration membrane of 200 dalton by molecular weight cut-off obtains concentrated solution; Concentrated solution obtains bioactive peptide finished product through lyophilize.
2. the preparation method of a kind of compound bioactive peptide of aquatic product protein-vegetable-protein according to claim 1, it is characterised in that:
Subtilis (Bacillussubtilis) HL-2 used in described step (1) is GDMCCNo:60001 at the deposit number of Guangdong Province's Culture Collection.
3. the preparation method of a kind of compound bioactive peptide of aquatic product protein-vegetable-protein according to claim 1, it is characterised in that:
Aquatic product protein in described step (2) is the one in leftovers of tilapia, krill, shrimp head, oyster, scallop body, cod tankage, squid tankage, Harengulazunasi (Bleeker) tankage, black carp tankage, silver carp tankage, grass carp tankage and stripped tuna tankage.
4. the preparation method of a kind of compound bioactive peptide of aquatic product protein-vegetable-protein according to claim 1, it is characterised in that:
Vegetable-protein in described step (2) is a kind of or wherein two or more mixture in dregs of beans, peanut meal, cottonseed meal and the dish dregs of rice.
5. the preparation method of a kind of compound bioactive peptide of aquatic product protein-vegetable-protein according to claim 1, it is characterised in that:
Nutrition in described step (3) is the mixture of Semen Maydis powder, food-grade calcium chloride, food grade iron protochloride and food grade phosphoric acid hydrogen two potassium composition.
6. the preparation method of a kind of compound bioactive peptide of aquatic product protein-vegetable-protein according to claim 1, it is characterised in that:
Nutraceutical add-on in described step (3) accounts for fermention medium total mass per-cent in the nutraceutical quality added, it is respectively: Semen Maydis powder 0.01%��5%, food-grade calcium chloride 0.01%��5%, food grade iron protochloride 0.01%��5%, food grade phosphoric acid hydrogen two potassium 0.01%��5%.
7. the preparation method of a kind of compound bioactive peptide of aquatic product protein-vegetable-protein according to claim 1, it is characterized in that: in described step (4), the inoculum size of subtilis (Bacillussubtilis) HL-2 seed liquor accounts for fermention medium total mass per-cent to access the quality of seed liquor, is 1%��15%.
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Cited By (9)
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| CN105961842A (en) * | 2016-07-13 | 2016-09-28 | 陈雨 | Compound shrimp peptide protein for feed and preparation process thereof |
| CN105996021A (en) * | 2016-06-29 | 2016-10-12 | 青岛浩大海洋保健食品有限公司 | Anti-aging marine protein healthcare product rich in amino acid and preparing method thereof |
| CN106036890A (en) * | 2016-06-29 | 2016-10-26 | 青岛浩大海洋保健食品有限公司 | Special female marine protein health product containing rich amino acids and preparation method thereof |
| CN106035984A (en) * | 2016-06-29 | 2016-10-26 | 青岛浩大海洋保健食品有限公司 | Ocean protein healthcare product rich in amino acid for teenagers and preparation method thereof |
| CN106072548A (en) * | 2016-06-29 | 2016-11-09 | 青岛浩大海洋保健食品有限公司 | A kind of rich in amino acid whose infant marine protein health product and preparation method thereof |
| CN106072665A (en) * | 2016-06-29 | 2016-11-09 | 青岛浩大海洋保健食品有限公司 | A kind of person in middle and old age are with aminoacid calcium-replenishing marine protein health product and preparation method thereof |
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| CN107467674A (en) * | 2017-06-22 | 2017-12-15 | 乐清瑞耀食品技术有限公司 | Compound based on duckbilled fish active peptides |
| CN112573961A (en) * | 2020-12-26 | 2021-03-30 | 南宁东恒华道生物科技有限责任公司 | Liquid micro-peptide fertilizer produced by composite enzymolysis of urban fresh garbage, production process and application thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105996021A (en) * | 2016-06-29 | 2016-10-12 | 青岛浩大海洋保健食品有限公司 | Anti-aging marine protein healthcare product rich in amino acid and preparing method thereof |
| CN106036890A (en) * | 2016-06-29 | 2016-10-26 | 青岛浩大海洋保健食品有限公司 | Special female marine protein health product containing rich amino acids and preparation method thereof |
| CN106035984A (en) * | 2016-06-29 | 2016-10-26 | 青岛浩大海洋保健食品有限公司 | Ocean protein healthcare product rich in amino acid for teenagers and preparation method thereof |
| CN106072548A (en) * | 2016-06-29 | 2016-11-09 | 青岛浩大海洋保健食品有限公司 | A kind of rich in amino acid whose infant marine protein health product and preparation method thereof |
| CN106072665A (en) * | 2016-06-29 | 2016-11-09 | 青岛浩大海洋保健食品有限公司 | A kind of person in middle and old age are with aminoacid calcium-replenishing marine protein health product and preparation method thereof |
| CN105961842A (en) * | 2016-07-13 | 2016-09-28 | 陈雨 | Compound shrimp peptide protein for feed and preparation process thereof |
| CN106173259A (en) * | 2016-07-13 | 2016-12-07 | 陈雨 | A kind of feedstuff compound fish peptide albumen and preparation technology thereof |
| CN107467674A (en) * | 2017-06-22 | 2017-12-15 | 乐清瑞耀食品技术有限公司 | Compound based on duckbilled fish active peptides |
| CN112573961A (en) * | 2020-12-26 | 2021-03-30 | 南宁东恒华道生物科技有限责任公司 | Liquid micro-peptide fertilizer produced by composite enzymolysis of urban fresh garbage, production process and application thereof |
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