CN103948137A - Complex dietary fiber solid beverage and preparation method thereof - Google Patents
Complex dietary fiber solid beverage and preparation method thereof Download PDFInfo
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- CN103948137A CN103948137A CN201410197567.2A CN201410197567A CN103948137A CN 103948137 A CN103948137 A CN 103948137A CN 201410197567 A CN201410197567 A CN 201410197567A CN 103948137 A CN103948137 A CN 103948137A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
- A23L33/22—Comminuted fibrous parts of plants, e.g. bagasse or pulp
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/152—Cereal germ products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a complex dietary fiber solid beverage and a preparation method thereof. The complex dietary fiber solid beverage is prepared by the following steps: grinding wheat bran and filtering through a 40-60-mesh sieve, adding water at a material-liquid ratio of 1:(6-12)g/ml and mixing, preserving heat at 50-60 DEG C for 4-6 hours under the condition that pH value is 4-6 and removing phytic acid; hydrolyzing through amylase and alkaline protease, centrifuging and drying precipitate at 60-80 DEG C for 6-10 hours, grinding and filtering through a 60-100-mesh sieve to obtain bran dietary fiber; grinding wheat germs and filtering through a 40-80-mesh sieve, adding water at a material-liquid ratio of 1:(6-12)g/ml and mixing, soaking for 1-2 hours, cooking for 10-30min, grinding into pulp, and hydrolyzing the wheat germs through amylase and papain to obtain wheat germ enzymatic hydrolysate; processing and homogenizing the enzymatic hydrolysate through a colloid mill, and spray-drying to obtain wheat germ powder; and uniformly mixing the wheat germ powder with wheat bran dietary fiber at a ratio of 1:(1-3) to obtain the complex dietary fiber solid beverage. The complex dietary fiber solid beverage disclosed by the invention is significant in blood glucose lowering function and high in application value.
Description
Technical field
The technical field that the present invention relates to food processing agricultural byproducts exploitation preparation, specifically, the present invention relates to technical field prepared by a kind of composite dietary solid beverage.
Background technology
Wheat is one of staple food crop of China and even extensive cultivation in the world, and the whole world has 43 countries taking wheat as staple food, accounts for 35%～40% of world's total population.Approximately 6.2 hundred million tons of world wheat annual productions, are only second to paddy, come second.Wheat is as one of three large staple food grains of China, and its cultivated area, total output and consumption figure all occupy first place in the world.
Wheat bran is as the accessory substance of wheat processing, and world's year processing capacity is 200,000,000 tons of left and right, approximately 7000～8,000 ten thousand tons of China's wheat bran annual productions, and these wheat brans are directly used as feed substantially, are rarely used in deep processing and comprehensive recycling, and economic worth is not high.Modern scientific research discovery, wheat bran, except containing the nutriments such as starch, protein, fat, vitamin, mineral matter, also contains abundant dietary fiber, is one of important sources of the active dietary fiber of high-quality.At present common wheat processing corporate boss will, by wheat bran as feed processing, cause the significant wastage of resource.And wheat embryo itself is nutritious, in wheat bran, dietary fiber content is high, as realizes the wheat bran of the comprehensive utilization of wheat processing byproduct, turns waste into wealth, significant.
Wheat embryo is the highest part of nutritive value in wheat, account for 2.5%～3.0% of whole wheat, the protein, fat, starch, crude fibre, multivitamin, mineral matter, trace element and some physiological activators that contain abundant and high-quality, be described as " the nutrition treasure-house that the mankind are natural " by nutritionists.The average recovery rate of China flour industry wheat embryo is 0.3%, the total resources of calculating accordingly China wheat embryo can reach 280～4,200,000 tons, but the existence of wheat embryo can have a strong impact on processing quality, baking quality and the storage life of flour, in Flour production process, the producer is mostly devoted to it farthest to remove, and be used as animal feed and some wine manufacturing industries together with wheat bran, thereby cause the significant wastage of resource.At present more visible reports that wheat embryo is utilized through enzymolysis, but the wheat germ powder of considerable upper preparation exists two problems, the one, instant capacity is poor, and the 2nd, exist to allow the offending wheat fishy smell of people, need badly and solve.If can make full use of this precious resources of wheat embryo, turn waste into wealth, set it as the requirement that nutraceutical or a kind of nourishing additive agent adapt to each age group people, its exploitation will have good economic benefit and wide market potential.
Common solid beverage raw material, auxiliary material through allotment, concentrated, dry and obtain, or by various drying raw materials through pulverizing, by certain Powdered, granular product after mixing than row.Solid beverage has the advantages such as the space of saving, easy to carry, easy storage, convenient transport, is more and more subject to liking of people.
Dietary fiber is called as the seventh-largest nutrient of human body, there is prevent colon cancer and constipation, reduction blood cholesterol levels, prevention and treat the physiological functions such as atherosclerotic and coronary heart disease, and to preventing the modern times " ciril disease " to have positive meaning as obesity, hyperlipemia, diabetes etc.Have no at present the new wheat-bran dietary fiber product of Development & Multipurpose use wheat bran and wheat embryo comprehensive exploitation, develop wheat bran and wheat embryo and prepare dietary fiber solid beverage, thereby increase the added value of wheat products, enrich diet fiber product market, can realize the comprehensive utilization to wheat resource, there is wide development space and meaning.
Summary of the invention
For the state of the art that has no at present Development & Multipurpose use wheat bran and wheat embryo comprehensive exploitation composite dietary solid beverage, the object of the invention is intended to the accessory substance with wheat processing, by utilizing wheat bran and wheat embryo, utilize wheat-bran dietary fiber and the wheat germ powder of preparation, determine both rational proportions by scientific experimentation, prepare composite dietary solid beverage according to the definite technique of experiment, the composite dietary solid beverage of preparation has obvious effect of lowering blood sugar, is with a wide range of applications.
The present invention specifically provides a kind of method of preparing composite dietary solid beverage, and concrete preparation method's step is as follows:
(1) will after wheat bran pulverizing, sieve through 40 order-60 orders, the ratio that is 1:6 to 1:12 with water weight ratio according to wheat bran adds water and mixes, and adjusting pH of mixed value with hydrochloric acid is 4-6, is incubated 4h-6h and removes phytic acid under 50 DEG C of-60 DEG C of conditions.
(2) adopt α-amylasehydrolysis to remove starch the enzyme that goes out in wheat bran in the wheat bran after above-mentioned removal phytic acid, diastatic action condition is: temperature 50 C-60 DEG C, time 0.5h-1.5h, solid-liquid ratio 1:8g/mL to 1:12g/mL, enzyme addition 20U/g-30U/g; Adopt hydrolysis by novo to remove protein the enzyme that goes out in wheat bran, the action condition of alkali protease is: 55 DEG C-65 DEG C of temperature, time 1h-3h, solid-liquid ratio 1:6g/mL to 1:10g/mL, pH value 9-10, enzyme addition 1200U/g-1500U/g.
(3) by the enzymolysis liquid centrifugal filtration in above-mentioned steps (2), to get precipitation and dry, temperature is 60 DEG C-80 DEG C, the time is 6h-10h.
(4) dried wheat-bran dietary fiber coarse crushing is crossed to 60 order-100 eye mesh screens, recycling micronizer is processed 2-3min, and its further dispersion and fining to granularity is reached to 250 order-300 orders, improves soluble dietary fibre content, obtains wheat-bran dietary fiber.
(5) by wheat germ grinding and sieving 40 order-80 orders, the ratio that is 1:6 to 1:12 with water weight ratio according to wheat germ adds water and mixes, and soaks after 1h-2h boiling 10min-30min deactivation.
(6) 2min that polishes for the first time of the wheat germ liquid after to above-mentioned steps (5) deactivation with beater, adopts respectively AMS and papain hydrolysis wheat embryo to obtain wheat embryo enzymolysis liquid; Diastatic action condition is: 55 DEG C-70 DEG C of temperature, time 0.5h-2.5h, solid-liquid ratio 1:8g/mL to 1:12g/mL, enzyme addition 20U/g-30U/g; The action condition of papain is: 55 DEG C-65 DEG C of temperature, time 1h-3h, solid-liquid ratio 1:10g/mL to 1:14g/mL, pH value 5-7, enzyme addition 3000U/g-4000U/g.
(7) enzymolysis liquid of being prepared by step (6) goes out after enzyme with the milling treatment of colloid 3min-5min of 50 DEG C-60 DEG C, then carries out second homogenate with high pressure homogenizer, and pressure is 20MPa-30MPa.
(8) the wheat germ enzymolysis liquid after step (7) homogeneous is sprayed and be dried, controlling EAT is 160 DEG C-180 DEG C, and leaving air temp is 70 DEG C-90 DEG C, and atomisation pressure is 0.5MPa-0.8MPa, obtains wheat germ powder.
(9) after mixing, the ratio that the wheat-bran dietary fiber of being prepared by step (4) is 1:1 to 1:3 with wheat germ powder prepared by step (8) according to weight ratio obtains composite dietary solid beverage.
By implementing, the present invention is above-mentioned provides concrete summary of the invention, can reach following technique effect.
Using the present invention is the accessory substance of selecting wheat processing, adopting wheat bran and wheat embryo is raw material, utilize modern high technology to prepare wheat-bran dietary fiber and wheat germ powder, through ultramicro grinding, processing can realize the modification to insoluble diedairy fiber, increase the content of soluble dietary fiber, wheat germ powder is after enzymolysis and spray drying treatment, and its nutritional labeling is comprehensive and be easier to absorption of human body, and both are prepared to composite dietary solid beverage after rational allocation by a certain percentage.Therefore develop composite dietary product, will realize the comprehensive utilization of wheat processing accessory substance, expand wheat processing industry, increase wheat products added value.
Brief description of the drawings
Fig. 1 is wheat-bran dietary fiber and wheat germ powder ratio chart.
Fig. 2 is each group rat body weight variation diagram during the composite dietary solid beverage of test preparation.
Fig. 3 is each group rat fasting blood-glucose value variation diagram during the composite dietary solid beverage of test preparation.
Fig. 4 is that the composite dietary solid beverage of test preparation finishes the glycogen content figure of rear each group of rat.
Fig. 5 is the affect figure of composite dietary on diabetes rat serum I nS of preparation.
Fig. 6 is the affect figure of composite dietary on diabetes rat change of serum C-P of preparation.
Fig. 7 is the affect figure of composite dietary on diabetes rat serum GSP of preparation.
Fig. 8 is the affect figure of composite dietary on diabetes rat serum T-AOC of preparation.
Detailed description of the invention
For embodiment, the present invention is described below, but the present invention is not limited to following embodiment.
embodiment mono-: the preparation of composite dietary solid beverage
The concrete preparation process of method of composite dietary solid beverage is as follows:
(1) will after wheat bran pulverizing, sieve through 40 order-60 orders, the ratio that is 1:6 to 1:12 with water weight ratio according to wheat bran adds water and mixes, and adjusting pH of mixed value with hydrochloric acid is 4-6, is incubated 4h-6h and removes phytic acid under 50 DEG C of-60 DEG C of conditions.
(2) adopt α-amylasehydrolysis to remove starch the enzyme that goes out in wheat bran in the wheat bran after above-mentioned removal phytic acid, diastatic action condition is: temperature 50 C-60 DEG C, time 0.5h-1.5h, solid-liquid ratio 1:8g/mL to 1:12g/mL, enzyme addition 20U/g-30U/g; Adopt hydrolysis by novo to remove protein the enzyme that goes out in wheat bran, the action condition of alkali protease is: 55 DEG C-65 DEG C of temperature, time 1h-3h, solid-liquid ratio 1:6g/mL to 1:10g/mL, pH value 9-10, enzyme addition 1200U/g-1500U/g.
(3) by the enzymolysis liquid centrifugal filtration in above-mentioned steps (2), to get precipitation and dry, temperature is 60 DEG C-80 DEG C, the time is 6h-10h.
(4) dried wheat-bran dietary fiber coarse crushing is crossed to 60 order-100 eye mesh screens, recycling micronizer is processed 2-3min, and its further dispersion and fining to granularity is reached to 250 order-300 orders, improves soluble dietary fibre content, obtains wheat-bran dietary fiber.
(5) by wheat germ grinding and sieving 40 order-80 orders, the ratio that is 1:6 to 1:12 with water weight ratio according to wheat germ adds water and mixes, and soaks after 1h-2h boiling 10min-30min deactivation.
(6) 2min that polishes for the first time of the wheat germ liquid after to above-mentioned steps (5) deactivation with beater, adopts respectively AMS and papain hydrolysis wheat embryo to obtain wheat embryo enzymolysis liquid; Diastatic action condition is: 55 DEG C-70 DEG C of temperature, time 0.5h-2.5h, solid-liquid ratio 1:8g/mL to 1:12g/mL, enzyme addition 20U/g-30U/g; The action condition of papain is: 55 DEG C-65 DEG C of temperature, time 1h-3h, solid-liquid ratio 1:10g/mL to 1:14g/mL, pH value 5-7, enzyme addition 3000U/g-4000U/g.
(7) enzymolysis liquid of being prepared by step (6) goes out after enzyme with the milling treatment of colloid 3min-5min of 50 DEG C-60 DEG C, then carries out second homogenate with high pressure homogenizer, and pressure is 20MPa-30MPa.
(8) the wheat germ enzymolysis liquid after step (7) homogeneous is sprayed and be dried, controlling EAT is 160 DEG C-180 DEG C, and leaving air temp is 70 DEG C-90 DEG C, and atomisation pressure is 0.5MPa-0.8MPa, obtains wheat germ powder.
(9) after mixing, the ratio that the wheat-bran dietary fiber of being prepared by step (4) is 1:1 to 1:3 with wheat germ powder prepared by step (8) according to weight ratio obtains composite dietary solid beverage.
The composite dietary solid beverage of preparing through above-mentioned technique has following attribute and good characteristic.
Balanced in nutrition and abundant, wherein protein content reaches 23.40%, and dietary fiber content reaches 29.52%.In supplementing the nutrition such as albumen for human body, regulate human metabolism function with dietary fiber, and there is effect of lowering blood sugar.In addition, this composite dietary puts goods on the market with the form of solid beverage, reconstitutes i.e. drink, convenient and swift.
embodiment bis-: the preparation of composite dietary solid beverage
(1) by wheat bran grinding and sieving 40 orders, the ratio that is 1:6 with water weight ratio according to wheat bran adds water and mixes, and adjusting pH of mixed value with hydrochloric acid is 4.5, under 50 DEG C of conditions, is incubated 6h.
(2) adopt α-amylasehydrolysis to remove starch the enzyme that goes out in wheat bran, diastatic action condition is: temperature is 50 DEG C, and the time is 1h, and solid-liquid ratio is 1:10g/mL, and enzyme addition is 20U/g; Adopt hydrolysis by novo to remove protein the enzyme that goes out in wheat bran, the action condition of alkali protease is: 55 DEG C of temperature, time 1.5h, solid-liquid ratio 1:6, pH value 9.5, enzyme addition 1200U/g.
(3) by enzymolysis liquid centrifugal filtration, to get precipitation and dry, temperature is 60 DEG C, the time is 10h.
(4) dried wheat-bran dietary fiber coarse crushing is crossed to 60 eye mesh screens, recycling micronizer is processed 2min, and its further dispersion and fining to granularity is reached to 250 orders, improves soluble dietary fibre content, obtains wheat-bran dietary fiber.
(5) wheat germ grinding and sieving 40 orders, the ratio that is 1:6 with water weight ratio according to wheat germ adds water and mixes, and soaks after 1h boiling 10min deactivation.
(6) with beater, step (5) is prepared to wheat germ liquid after the deactivation 2min that polishes for the first time, adopt respectively AMS and papain hydrolysis wheat embryo to obtain wheat embryo enzymolysis liquid; Diastatic action condition is: 55 DEG C of temperature, time 0.5h, solid-liquid ratio 1:12g/mL, enzyme addition 30U/g; The action condition of papain is: 65 DEG C of temperature, time 1h, solid-liquid ratio 1:14 g/mL, pH value 5, enzyme addition 3000U/g.
(7) enzymolysis liquid is gone out after enzyme with the milling treatment of colloid 5min of 50 DEG C, then carry out second homogenate with high pressure homogenizer, pressure is 20MPa.
(8) the wheat germ enzymolysis liquid after homogeneous is sprayed and be dried, controlling EAT is 160 DEG C, and leaving air temp is 90 DEG C, and atomisation pressure is 0.5MPa, obtains wheat germ powder.
(9) the ratio allotment that wheat germ powder prepared by the wheat-bran dietary fiber of being prepared by step (4) and step (8) is 1:1 according to weight ratio, mixes, and obtains composite dietary solid beverage.
embodiment tri-: the preparation of composite dietary solid beverage
(1) wheat bran grinding and sieving 60 orders, the ratio that is 1:10 with water weight ratio according to wheat bran adds water and mixes, adjusting pH of mixed value with hydrochloric acid is 5.5, under 55 DEG C of conditions, be incubated 5h, adopt α-amylasehydrolysis to remove starch the enzyme that goes out in wheat bran, diastatic action condition is: 55 DEG C of temperature, time 0.5h, solid-liquid ratio 1:8 g/mL, enzyme addition 25U/g; Adopt hydrolysis by novo to remove protein the enzyme that goes out in wheat bran, the action condition of alkali protease is: temperature 60 C, time 2.5h, solid-liquid ratio 1:8 g/mL, pH value 9, enzyme addition 1400U/g, by enzymolysis liquid centrifugal filtration, to get precipitation and dry, temperature is 70 DEG C, time is 8h, dried wheat-bran dietary fiber coarse crushing is crossed to 80 eye mesh screens, and recycling micronizer is processed 3min, and its further dispersion and fining to granularity is reached to 280 orders, improve soluble dietary fibre content, obtain wheat-bran dietary fiber.
(2) by wheat germ grinding and sieving 60 orders, the ratio that is 1:12 with water weight ratio according to wheat germ adds water and mixes, soak after 1.5h, boiling 30min deactivation, wheat germ liquid with beater after to the deactivation 2min that polishes for the first time, adopts respectively AMS and papain hydrolysis wheat embryo to obtain wheat embryo enzymolysis liquid; Diastatic action condition is: 65 DEG C of temperature, time 1h, solid-liquid ratio 1:8g/mL, enzyme addition 25U/g; The action condition of papain is: temperature 60 C, and time 2h, solid-liquid ratio 1:12 g/mL, pH value 5.5, enzyme addition 3500U/g, goes out after enzyme enzymolysis liquid with the milling treatment of colloid 4min of 60 DEG C, then carries out second homogenate with high pressure homogenizer, and pressure is 30Mpa; Wheat germ enzymolysis liquid after homogeneous is sprayed dry, controlling EAT is 180 DEG C, and leaving air temp is 70 DEG C, and atomisation pressure is 0.6MPa, obtains wheat germ powder.
(3) the ratio allotment that wheat germ powder prepared by the wheat-bran dietary fiber of being prepared by step (1) and step (2) is 1:2 according to weight ratio, mixes, and obtains composite dietary solid beverage.
embodiment tetra-: the preparation of composite dietary solid beverage
(1) preparation of wheat-bran dietary fiber: wheat bran grinding and sieving 60 orders, the ratio that is 1:12 with water weight ratio according to wheat bran adds water and mixes, adjusting pH of mixed value with hydrochloric acid is 6, under 60 DEG C of conditions, be incubated 4h, adopt α-amylasehydrolysis to remove starch the enzyme that goes out in wheat bran, diastatic action condition is: temperature 60 C, time 1.5h, solid-liquid ratio 1:12 g/mL, enzyme addition 30U/g; Adopt hydrolysis by novo to remove protein the enzyme that goes out in wheat bran, the action condition of alkali protease is: 65 DEG C of temperature, time 3h, solid-liquid ratio 1:10 g/mL, pH value 10, enzyme addition 1500U/g, by enzymolysis liquid centrifugal filtration, to get precipitation and dry, temperature is 80 DEG C, time is 6h, dried wheat-bran dietary fiber coarse crushing is crossed to 100 eye mesh screens, and recycling micronizer is processed 2min, and its further dispersion and fining to granularity is reached to 300 orders, improve soluble dietary fibre content, obtain wheat-bran dietary fiber.
(2) preparation of wheat germ powder: by wheat germ grinding and sieving 80 orders, the ratio that is 1:10 with water weight ratio according to wheat germ adds water and mixes, soak after 2h, boiling 20min deactivation, wheat germ liquid with beater after to the deactivation 2min that polishes for the first time, adopts respectively AMS and papain hydrolysis wheat embryo to obtain wheat embryo enzymolysis liquid; Diastatic action condition is: temperature 60 C, time 2.5h, solid-liquid ratio 1:10 g/mL, enzyme addition 20U/g; The action condition of papain is: 55 DEG C of temperature, time 3h, solid-liquid ratio 1:10 g/mL, pH value 6.5, enzyme addition 4000U/g, goes out after enzyme enzymolysis liquid with the milling treatment of colloid 3min of 55 DEG C, then carries out second homogenate with high pressure homogenizer, pressure is 25Mpa, wheat germ enzymolysis liquid after homogeneous is sprayed dry, controlling EAT is 170 DEG C, and leaving air temp is 80 DEG C, atomisation pressure is 0.7MPa, obtains wheat germ powder.
(3) the ratio allotment that wheat germ powder prepared by the wheat-bran dietary fiber of being prepared by step (1) and step (2) is 1:3 according to weight ratio, mixes, and obtains composite dietary solid beverage.
embodiment five: the selection of composite dietary solid beverage primary raw material
About the ratio-dependent of wheat germ powder and wheat bran dietary fiber powder, select the two ratio to be respectively 3:1,2:1,1:1,1:2,1:3 as 5 samples, choose 5 subjective appreciation personnel, by sensory evaluation method, determine optimal proportion with final score.Sensory evaluation table is as following table 1:
Table 1: the composite dietary solid beverage sensory evaluation table of preparation
5 samples respectively in triplicate, are averaged as final score.Results of sensory evaluation is table 1 as above, known referring to accompanying drawing 1, and the 3rd sample is wheat-bran dietary fiber and wheat germ powder ratio while being 1:1, and subjective appreciation score is the highest, so the optimal proportion of the two is decided to be 1:1.
embodiment six: composite dietary solid beverage effect experiment
Object: carry out the research to regulating blood glucose levels of composite dietary solid beverage product that above-described embodiment provides with the rat experiment of feeding, thereby checking obtains through the above-mentioned composite dietary solid beverage preparation technology who provides corresponding effect that composite dietary solid beverage possesses.
Experimental design: to supplying examination rat oral gavage composite dietary solid beverage, by measuring the indexs such as rat body weight, fasting blood sugar, TAC, insulin, taking two groups, normal, model as contrast, observe composite dietary to the impact for examination rat blood sugar level.
1, experiment material.
1.1 animals used as test selection SPF (
specific pathogen Free) level SD (
sprague Dswley) 100 of rats, male and female half and half, body weight 190 ± 20g, is purchased from Xinjiang Medicine University's animal center.
1.2 basal feeds are purchased from Xinjiang Medicine University's animal center, configure with reference to GB14924.3-2001.
The gavage dosage of 1.3 dietary fibers and mode specify with reference to intake according to China's dietary nutrition of urban residents element, the average daily intake of dietary fiber is 30g, gavage metering is according to 2 times of calculating of the average daily DF recommended intake (30g) of adult (60g), and calculate with rat average weight 200g, the composite dietary that accurately takes 0.2g is suspended in the stable fiber suspension of formation in the sodium alginate soln of 2mL (SAS).
2 experimental techniques.
The foundation of 2.1 diabetes model rats
Choose at random 50 of rats, male and female half and half are taked fasting to can't help water and are raised in 12h.Use citric acid-citrated milk cushioning liquid (0.1mmol/L, Ph4.2) configuration 2mg/Mlde Streptozotocin (STZ) solution (matching while using) simultaneously.Then, every rat is according to the dosage of the 55mg/kg STZ fresh solution that injection prepares on an empty stomach, after injection, all animals are taked free diet, after the dead sample 6d of stability, get blood in afterbody, separation of serum is measured its fasting blood-glucose, the rat of blood glucose value >=11.1mmol/L is defined as to diabetes rat, and below standard person appends a STZ of injection.
The grouping of 2.2 rats and processing
The design of diabetes rat according to the form below is divided into 2 processing (15/group) at random, i.e. model control group (MC) and dietary fiber group (DF), establishes 15 healthy rats in addition as Normal group (NC).Duration of test is respectively organized rat and is all taked freely ingest and drink water every day, and gives different gavage processing simultaneously.
Table 2: experiment grouping and the mode of feeding
According to upper table 2 experiment grouping and the carrying out of mode of feeding, respectively organize rat and carry out the gavage raising of 3 weeks continuously once a day.Experimental session will note observing the growing states such as each group of rats eating, drinking-water, urine amount and the state of an illness, and respectively at 0,1,2,3 weekends by rat fasting 12h, after weighing in by cut tail blood collection method measure fasting blood sugar.All rat fasting 12h in the time of experiment 21d, get the about 5mL of blood by extracing eyeball, and blood normal temperature leaves standstill after 2.5h, and 2000g refrigerated centrifuge 15min gets upper strata liquid and obtains serum, for the mensuration of the indexs such as serum insulin.Then dissect and put to death rat, win respectively liver and the musculature mensuration for hepatic glycogen and muscle glycogen content.
The mensuration of 2.3 rat physical signs.
2.3.1 the mensuration of rat body weight: the rat after fasting 12h is placed on balance, records rat body weight.
2.3.2 the mensuration of fasting blood sugar: carry out weekly rat tails blood sampling, utilize blood glucose meter to carry out the mensuration of fasting blood sugar.
2.3.3 the mensuration of hepatic glycogen and muscle glycogen content: utilize anthrone colorimetric method to measure.
2.3.4 four indexs of the mensuration of insulin (InS), C-peptide (C-P), glycated serum protein (GSP) and TAC (T-AOC) are all with reference to the explanation of kit of buying.
3, results and analysis
3.1 impacts of composite dietary solid beverage on diabetes rat body weight:
From accompanying drawing 2, along with the prolongation of feeding time, NC group body weight increases gradually, and the body weight of MC group rat reduces gradually, and this is because the diabetes rat state of an illness constantly increases the weight of, and causes Body weight loss and no longer increases.DF group rat feed 1 week afterwards body weight do not increase, along with the increase of feeding time, body weight starts remarkable increase, substantially can reach the body weight level same with normal rat by the 3rd week, illustrate that the composite dietary solid beverage of feeding can suppress the rat body weight decline that diabetes cause, to a certain extent can diabetes-alleviating symptom.
3.2 impacts of composite dietary solid beverage on diabetes rat fasting blood sugar:
As shown in Figure 3, between whole feed period, the fasting blood sugar kept stable of rats in normal control group.MC group fasting blood sugar in the time of first week is in a slight decrease, sustainable growths always in three weeks afterwards, and dietary fiber group rat blood glucose value after feeding 1 week significantly reduces, substantially can reach normal level, the composite dietary solid beverage of feeding as seen has hypoglycemic activity to diabetes rat.
The impact of 3.3 composite dietary solid beverages on diabetes rat hepatic glycogen and muscle glycogen content:
From accompanying drawing 4, the hepatic glycogen content of rats in normal control group and muscle glycogen content are always lower, and in diabetes rat, the dry glycogen content of rat of model control group and muscle glycogen content are all in highest level, this is that glycogen content increases suddenly because diabetes cause that in rat body, glycogen metabolism gets muddled.The fed diabetes rat of composite dietary, DF group rats'liver glycogen content significantly reduces, and muscle glycogen content is also reduced to normal level substantially.This just illustrates that this composite dietary solid beverage has the glycogen metabolism function of improving diabetes rat, further makes glycogen content return to normal level.
The impact of 3.4 composite dietary solid beverages on diabetes rat serum I nS, C-P, GSP and T-AOC:
Accompanying drawing 5 to accompanying drawing 8 has shown respectively raises InS, the C-P, GSP and the T-AOC level that finish rear 3 groups of rats, and wherein insulin (InS), C-peptide (C-P), glycated serum protein (GSP) and TAC (T-AOC) are all common detection indexs; From accompanying drawing 5, to accompanying drawing 8, the rat of model control group is because of the aggravation of the diabetes state of an illness, and its front 3 indexs are the highest level of 3 groups; And front 3 indexs of dietary fiber group rat all decline to some extent, and be more or less the same with normal level.From accompanying drawing 8, show that the TAC of dietary fiber group rat is all higher than the level of normal group and model group rat.Visible, the certain adjustable insulin of composite dietary solid beverage, C-P and the GSP level of preparation.
conclusion:by experimental rat being carried out to the detection of a series of indexs such as body weight, fasting blood sugar, fully show to adopt the composite dietary solid beverage of the above-mentioned preparation of the present invention really to there is effect of lowering blood sugar preferably.
More than setting forth the embodiment in the present invention, is not that the present invention is done to other formal restriction, and those skilled in the art person can change equivalent embodiment to the technology contents of above-mentioned elaboration.But as long as not departing from technical solution of the present invention content, according to technical spirit of the present invention, just above example is carried out to simple modification, equivalent variations and remodeling, still belong to the protection domain of the technology of the present invention.
Claims (1)
1. a composite dietary solid beverage, after mixing, the ratio that composite dietary solid beverage is 1:1 to 1:3 by wheat-bran dietary fiber and wheat germ powder according to weight ratio prepares, it is characterized in that, composite dietary solid beverage obtains by following concrete preparation method:
(1) will after wheat bran pulverizing, sieve through 40 order-60 orders, the ratio that is 1:6 to 1:12 with water weight ratio according to wheat bran adds water and mixes, and adjusting pH of mixed value with hydrochloric acid is 4-6, is incubated 4h-6h and removes phytic acid under 50 DEG C of-60 DEG C of conditions;
(2) adopt α-amylasehydrolysis to remove starch the enzyme that goes out in wheat bran in the wheat bran after above-mentioned removal phytic acid, diastatic action condition is: temperature 50 C-60 DEG C, time 0.5h-1.5h, solid-liquid ratio 1:8g/mL to 1:12g/mL, enzyme addition 20U/g-30U/g; Adopt hydrolysis by novo to remove protein the enzyme that goes out in wheat bran, the action condition of alkali protease is: 55 DEG C-65 DEG C of temperature, time 1h-3h, solid-liquid ratio 1:6g/mL to 1:10g/mL, pH value 9-10, enzyme addition 1200U/g-1500U/g;
(3) by the enzymolysis liquid centrifugal filtration in above-mentioned steps (2), to get precipitation and dry, temperature is 60 DEG C-80 DEG C, the time is 6h-10h;
(4) dried wheat-bran dietary fiber coarse crushing is crossed to 60 order-100 eye mesh screens, recycling micronizer is processed 2-3min, and its further dispersion and fining to granularity is reached to 250 order-300 orders, improves soluble dietary fibre content, obtains wheat-bran dietary fiber;
(5) by wheat germ grinding and sieving 40 order-80 orders, the ratio that is 1:6 to 1:12 with water weight ratio according to wheat germ adds water and mixes, and soaks after 1h-2h boiling 10min-30min deactivation;
(6) 2min that polishes for the first time of the wheat germ liquid after to above-mentioned steps (5) deactivation with beater, adopts respectively AMS and papain hydrolysis wheat embryo to obtain wheat embryo enzymolysis liquid; Diastatic action condition is: 55 DEG C-70 DEG C of temperature, time 0.5h-2.5h, solid-liquid ratio 1:8g/mL to 1:12g/mL, enzyme addition 20U/g-30U/g; The action condition of papain is: 55 DEG C-65 DEG C of temperature, time 1h-3h, solid-liquid ratio 1:10g/mL to 1:14g/mL, pH value 5-7, enzyme addition 3000U/g-4000U/g;
(7) enzymolysis liquid of being prepared by step (6) goes out after enzyme with the milling treatment of colloid 3min-5min of 50 DEG C-60 DEG C, then carries out second homogenate with high pressure homogenizer, and pressure is 20MPa-30Mpa;
(8) the wheat germ enzymolysis liquid after step (7) homogeneous is sprayed and be dried, controlling EAT is 160 DEG C-180 DEG C, and leaving air temp is 70 DEG C-90 DEG C, and atomisation pressure is 0.5MPa-0.8MPa, obtains wheat germ powder.
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